Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b5/bb77183bdb7f9026c299633d9ba972/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0f/3075612d2ed405a2b0aedbd22c6861/T21-D0003376/0002.T21-D0003376_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b5/bb77183bdb7f9026c299633d9ba972/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0f/3075612d2ed405a2b0aedbd22c6861/T21-D0003376/0002.T21-D0003376_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1889.264 CPU sec, 65.434 real sec
[M::process] read 676746 sequences (100000264 bp) in 0.918 CPU sec, 0.921 real sec...
[M::process] read 676466 sequences (100000014 bp) in 1.635 CPU sec, 1.665 real sec...
[M::process] read 676414 sequences (100000141 bp) in 1.450 CPU sec, 1.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288241, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.39, 109.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676348 sequences (100000129 bp) in 1.438 CPU sec, 1.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288043, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.79, 115.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288472, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.25, 113.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676746 reads in 272.027 CPU sec, 8.958 real sec
[M::mem_process_seqs] Processed 676414 reads in 173.852 CPU sec, 5.850 real sec
[M::mem_process_seqs] Processed 676466 reads in 181.270 CPU sec, 6.137 real sec
sorting block 0, #reads = 1081887 mem = 536879114 peak = 630091776
[M::process] read 676034 sequences (100000025 bp) in 1.780 CPU sec, 1.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287771, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.63, 119.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 19, 49)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 129)
[M::mem_pestat] mean and std.dev: (24.17, 17.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 169)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676096 sequences (100000102 bp) in 1.385 CPU sec, 1.409 real sec...
[M::process] read 676306 sequences (100000182 bp) in 1.391 CPU sec, 1.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288240, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.46, 122.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (662, 1725, 2317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5627)
[M::mem_pestat] mean and std.dev: (1059.64, 898.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7282)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676348 reads in 183.627 CPU sec, 5.856 real sec
sorting block 1, #reads = 1082056 mem = 536877886 peak = 842428416
[M::mem_process_seqs] Processed 676034 reads in 103.844 CPU sec, 3.309 real sec
sorting block 2, #reads = 1081709 mem = 536879170 peak = 952352768
[M::process] read 676478 sequences (100000297 bp) in 1.894 CPU sec, 1.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287465, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (242.97, 122.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676698 sequences (100000052 bp) in 1.416 CPU sec, 1.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288261, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.90, 120.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 38, 89)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 201)
[M::mem_pestat] mean and std.dev: (48.62, 33.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 257)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288385, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.32, 112.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676096 reads in 272.323 CPU sec, 8.782 real sec
[M::mem_process_seqs] Processed 676306 reads in 189.808 CPU sec, 6.183 real sec
[M::mem_process_seqs] Processed 676478 reads in 197.355 CPU sec, 6.480 real sec
sorting block 3, #reads = 1081638 mem = 536879845 peak = 952352768
[M::process] read 676546 sequences (100000279 bp) in 1.667 CPU sec, 1.928 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288459, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.08, 111.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 91, 125)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327)
[M::mem_pestat] mean and std.dev: (79.50, 62.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 428)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676478 sequences (100000058 bp) in 1.784 CPU sec, 2.071 real sec...
sorting block 4, #reads = 1082165 mem = 536880234 peak = 963485696
[M::process] read 676496 sequences (100000097 bp) in 1.314 CPU sec, 1.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288469, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.06, 113.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 266, 828)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2328)
[M::mem_pestat] mean and std.dev: (343.50, 331.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3078)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676698 reads in 187.633 CPU sec, 6.100 real sec
[M::mem_process_seqs] Processed 676546 reads in 105.338 CPU sec, 3.375 real sec
sorting block 5, #reads = 1082159 mem = 536877630 peak = 963485696
[M::process] read 676254 sequences (100000072 bp) in 1.674 CPU sec, 1.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288274, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.58, 114.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676770 sequences (100000266 bp) in 1.402 CPU sec, 1.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288185, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.28, 117.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 113, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1703)
[M::mem_pestat] mean and std.dev: (231.82, 231.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2262)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288270, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.14, 116.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 354, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1376)
[M::mem_pestat] mean and std.dev: (241.77, 272.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1822)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676478 reads in 269.613 CPU sec, 8.666 real sec
[M::mem_process_seqs] Processed 676496 reads in 189.361 CPU sec, 6.106 real sec
[M::mem_process_seqs] Processed 676254 reads in 197.509 CPU sec, 6.389 real sec
sorting block 6, #reads = 1082003 mem = 536878311 peak = 963485696
[M::process] read 676456 sequences (100000088 bp) in 2.003 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288065, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.73, 110.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1081961 mem = 536878234 peak = 963485696
[M::process] read 676408 sequences (100000021 bp) in 1.905 CPU sec, 2.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288353, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.95, 114.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676198 sequences (100000120 bp) in 1.550 CPU sec, 1.545 real sec...
[M::mem_process_seqs] Processed 676770 reads in 184.311 CPU sec, 5.945 real sec
[M::mem_process_seqs] Processed 676456 reads in 103.866 CPU sec, 3.294 real sec
sorting block 8, #reads = 1082379 mem = 536878304 peak = 963485696
[M::process] read 675944 sequences (100000229 bp) in 2.014 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288265, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.08, 116.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (362, 448, 2061)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5459)
[M::mem_pestat] mean and std.dev: (694.70, 736.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7158)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675964 sequences (100000169 bp) in 1.420 CPU sec, 1.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287700, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (240.54, 120.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 10, 28)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 80)
[M::mem_pestat] mean and std.dev: (11.78, 10.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 106)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287363, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (244.73, 124.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676408 reads in 269.253 CPU sec, 8.651 real sec
[M::mem_process_seqs] Processed 676198 reads in 190.285 CPU sec, 6.132 real sec
[M::mem_process_seqs] Processed 675944 reads in 198.269 CPU sec, 6.417 real sec
sorting block 9, #reads = 1082697 mem = 536879702 peak = 963485696
[M::process] read 676162 sequences (100000119 bp) in 1.916 CPU sec, 2.148 real sec...
sorting block 10, #reads = 1081894 mem = 536879454 peak = 967061504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287414, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.34, 123.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 20, 600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1782)
[M::mem_pestat] mean and std.dev: (89.12, 193.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2373)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677024 sequences (100000279 bp) in 1.835 CPU sec, 2.059 real sec...
[M::process] read 676664 sequences (100000253 bp) in 1.441 CPU sec, 1.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287645, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.37, 120.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 584, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1941)
[M::mem_pestat] mean and std.dev: (478.43, 353.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2571)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675964 reads in 192.506 CPU sec, 6.224 real sec
[M::mem_process_seqs] Processed 676162 reads in 108.534 CPU sec, 3.467 real sec
sorting block 11, #reads = 1081915 mem = 536877069 peak = 967061504
[M::process] read 676516 sequences (100000233 bp) in 1.878 CPU sec, 2.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288465, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.40, 107.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676446 sequences (100000050 bp) in 1.422 CPU sec, 1.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287770, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.43, 113.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 9, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244)
[M::mem_pestat] mean and std.dev: (53.00, 77.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 362)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288303, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.87, 115.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677024 reads in 273.614 CPU sec, 8.791 real sec
[M::mem_process_seqs] Processed 676664 reads in 191.914 CPU sec, 6.157 real sec
[M::mem_process_seqs] Processed 676516 reads in 199.460 CPU sec, 6.428 real sec
sorting block 12, #reads = 1082336 mem = 536879070 peak = 967061504
sorting block 13, #reads = 1082627 mem = 536878858 peak = 967061504
[M::process] read 676188 sequences (100000061 bp) in 2.077 CPU sec, 2.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287899, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.34, 117.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 113, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1443)
[M::mem_pestat] mean and std.dev: (241.56, 251.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1899)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676502 sequences (100000121 bp) in 1.840 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287557, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.12, 119.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676652 sequences (100000038 bp) in 1.432 CPU sec, 1.460 real sec...
[M::mem_process_seqs] Processed 676446 reads in 187.845 CPU sec, 6.068 real sec
[M::mem_process_seqs] Processed 676188 reads in 106.763 CPU sec, 3.421 real sec
sorting block 14, #reads = 1082680 mem = 536878119 peak = 967061504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287891, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.28, 114.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676736 sequences (100000028 bp) in 2.195 CPU sec, 2.366 real sec...
[M::process] read 676682 sequences (100000167 bp) in 1.479 CPU sec, 1.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288366, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (232.46, 111.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (63, 813, 813)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2313)
[M::mem_pestat] mean and std.dev: (488.30, 444.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3063)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676502 reads in 184.959 CPU sec, 5.920 real sec
[M::mem_process_seqs] Processed 676652 reads in 104.801 CPU sec, 3.355 real sec
sorting block 15, #reads = 1081423 mem = 536877708 peak = 967061504
sorting block 16, #reads = 1082378 mem = 536879077 peak = 967061504
[M::process] read 676292 sequences (100000171 bp) in 1.837 CPU sec, 1.936 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288136, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.35, 110.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676114 sequences (100000024 bp) in 1.472 CPU sec, 1.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288186, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.04, 113.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676736 reads in 186.778 CPU sec, 6.007 real sec
[M::mem_process_seqs] Processed 676682 reads in 105.258 CPU sec, 3.387 real sec
sorting block 17, #reads = 1082129 mem = 536878468 peak = 967061504
[M::process] read 676252 sequences (100000064 bp) in 1.815 CPU sec, 1.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288104, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.11, 117.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676388 sequences (100000247 bp) in 1.536 CPU sec, 1.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287551, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (241.18, 121.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287947, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.90, 120.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676292 reads in 280.091 CPU sec, 9.009 real sec
[M::mem_process_seqs] Processed 676252 reads in 196.649 CPU sec, 6.324 real sec
[M::mem_process_seqs] Processed 676114 reads in 204.849 CPU sec, 6.622 real sec
sorting block 18, #reads = 1082624 mem = 536879269 peak = 967061504
[M::process] read 676840 sequences (100000176 bp) in 2.246 CPU sec, 2.506 real sec...
sorting block 19, #reads = 1082227 mem = 536878488 peak = 968216576
[M::process] read 676516 sequences (100000169 bp) in 1.845 CPU sec, 2.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288397, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.30, 109.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676378 sequences (100000071 bp) in 1.506 CPU sec, 1.654 real sec...
[M::mem_process_seqs] Processed 676840 reads in 104.061 CPU sec, 3.318 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287815, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.16, 115.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676364 sequences (100000061 bp) in 1.569 CPU sec, 1.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287871, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.83, 114.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676388 reads in 290.889 CPU sec, 9.397 real sec
[M::mem_process_seqs] Processed 676516 reads in 116.282 CPU sec, 3.755 real sec
sorting block 20, #reads = 1082509 mem = 536879602 peak = 968216576
sorting block 21, #reads = 1082039 mem = 536878577 peak = 968216576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287833, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.41, 117.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 11, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (90.64, 100.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676188 sequences (100000095 bp) in 2.242 CPU sec, 2.501 real sec...
[M::process] read 676940 sequences (100000051 bp) in 1.432 CPU sec, 1.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287859, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.81, 117.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 252, 940)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2818)
[M::mem_pestat] mean and std.dev: (228.62, 303.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3757)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676378 reads in 191.028 CPU sec, 6.144 real sec
[M::mem_process_seqs] Processed 676364 reads in 106.648 CPU sec, 3.432 real sec
sorting block 22, #reads = 1082655 mem = 536877520 peak = 968216576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287776, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (238.87, 119.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676972 sequences (100000211 bp) in 1.998 CPU sec, 2.533 real sec...
[M::process] read 677022 sequences (100000156 bp) in 1.352 CPU sec, 1.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288149, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.90, 108.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676188 reads in 189.096 CPU sec, 6.110 real sec
[M::mem_process_seqs] Processed 676940 reads in 105.051 CPU sec, 3.374 real sec
sorting block 23, #reads = 1081723 mem = 536879772 peak = 968216576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288098, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.68, 105.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676642 sequences (100000255 bp) in 2.315 CPU sec, 2.485 real sec...
[M::process] read 676480 sequences (100000025 bp) in 1.538 CPU sec, 1.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288738, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.85, 105.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676972 reads in 188.197 CPU sec, 6.032 real sec
[M::mem_process_seqs] Processed 677022 reads in 103.832 CPU sec, 3.338 real sec
sorting block 24, #reads = 1082241 mem = 536883127 peak = 968216576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288451, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.22, 110.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676474 sequences (100000117 bp) in 1.725 CPU sec, 2.114 real sec...
[M::process] read 676496 sequences (100000079 bp) in 1.450 CPU sec, 1.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287841, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.55, 113.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676642 reads in 190.187 CPU sec, 6.172 real sec
[M::mem_process_seqs] Processed 676480 reads in 106.576 CPU sec, 3.430 real sec
sorting block 25, #reads = 1082982 mem = 536877417 peak = 981667840
sorting block 26, #reads = 1082594 mem = 536880455 peak = 1016619008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287483, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.85, 114.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (103, 103, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1762)
[M::mem_pestat] mean and std.dev: (225.21, 248.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2315)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676680 sequences (100000008 bp) in 2.230 CPU sec, 2.511 real sec...
[M::process] read 676674 sequences (100000061 bp) in 1.520 CPU sec, 1.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287617, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.19, 114.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676474 reads in 191.669 CPU sec, 6.166 real sec
[M::process] read 676588 sequences (100000181 bp) in 1.479 CPU sec, 1.486 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288330, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.92, 109.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676680 reads in 101.702 CPU sec, 3.384 real sec
sorting block 27, #reads = 1082091 mem = 536881032 peak = 1016619008
[M::mem_process_seqs] Processed 676496 reads in 241.892 CPU sec, 7.887 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288315, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.41, 108.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 15, 30)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 84)
[M::mem_pestat] mean and std.dev: (17.20, 18.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 111)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676408 sequences (100000279 bp) in 1.894 CPU sec, 2.301 real sec...
[M::process] read 676178 sequences (100000004 bp) in 1.471 CPU sec, 1.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287988, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.29, 112.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676674 reads in 197.601 CPU sec, 6.420 real sec
sorting block 28, #reads = 1082487 mem = 536877792 peak = 1016619008
[M::mem_process_seqs] Processed 676588 reads in 106.605 CPU sec, 3.497 real sec
sorting block 29, #reads = 1082471 mem = 536881619 peak = 1019686912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287690, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.93, 115.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676224 sequences (100000215 bp) in 1.967 CPU sec, 2.256 real sec...
[M::process] read 676980 sequences (100000237 bp) in 1.497 CPU sec, 1.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287785, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (238.77, 119.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676408 reads in 189.836 CPU sec, 6.194 real sec
[M::mem_process_seqs] Processed 676178 reads in 107.044 CPU sec, 3.416 real sec
sorting block 30, #reads = 1082058 mem = 536879644 peak = 1019686912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288168, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (239.04, 119.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676806 sequences (100000069 bp) in 2.249 CPU sec, 2.508 real sec...
[M::process] read 676734 sequences (100000288 bp) in 1.529 CPU sec, 1.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287755, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.65, 112.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 208, 1052)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3088)
[M::mem_pestat] mean and std.dev: (416.75, 452.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4106)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676224 reads in 191.546 CPU sec, 6.198 real sec
[M::mem_process_seqs] Processed 676980 reads in 108.512 CPU sec, 3.505 real sec
sorting block 31, #reads = 1081787 mem = 536879848 peak = 1019686912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288061, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.95, 115.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676426 sequences (100000267 bp) in 1.925 CPU sec, 2.223 real sec...
[M::process] read 676214 sequences (100000234 bp) in 1.386 CPU sec, 1.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287885, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.65, 115.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 195, 195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525)
[M::mem_pestat] mean and std.dev: (116.89, 76.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676806 reads in 192.901 CPU sec, 6.280 real sec
[M::mem_process_seqs] Processed 676734 reads in 106.704 CPU sec, 3.436 real sec
sorting block 32, #reads = 1082269 mem = 536879894 peak = 1019686912
[M::process] read 676182 sequences (100000027 bp) in 2.062 CPU sec, 2.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287563, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.58, 118.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1082384 mem = 536878066 peak = 1034162176
[M::process] read 676440 sequences (100000036 bp) in 1.397 CPU sec, 1.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287192, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (242.56, 123.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 960, 960)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2368)
[M::mem_pestat] mean and std.dev: (544.00, 417.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3072)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676426 reads in 190.103 CPU sec, 6.136 real sec
[M::mem_process_seqs] Processed 676214 reads in 105.751 CPU sec, 3.400 real sec
sorting block 34, #reads = 1081642 mem = 536878373 peak = 1034162176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287293, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (242.44, 123.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676850 sequences (100000100 bp) in 2.049 CPU sec, 2.310 real sec...
[M::process] read 676924 sequences (100000006 bp) in 1.499 CPU sec, 1.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287788, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.98, 119.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676182 reads in 191.691 CPU sec, 6.165 real sec
[M::mem_process_seqs] Processed 676440 reads in 106.081 CPU sec, 3.397 real sec
sorting block 35, #reads = 1081542 mem = 536878007 peak = 1034162176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287829, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.89, 110.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676822 sequences (100000215 bp) in 2.367 CPU sec, 2.631 real sec...
[M::process] read 676800 sequences (100000068 bp) in 1.602 CPU sec, 1.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287830, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.54, 109.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676850 reads in 190.006 CPU sec, 6.114 real sec
[M::mem_process_seqs] Processed 676924 reads in 108.270 CPU sec, 3.472 real sec
sorting block 36, #reads = 1081844 mem = 536878051 peak = 1034162176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287919, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.96, 110.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676582 sequences (100000164 bp) in 1.990 CPU sec, 2.327 real sec...
[M::process] read 676770 sequences (100000085 bp) in 1.518 CPU sec, 1.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287950, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.90, 113.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 286, 2189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6485)
[M::mem_pestat] mean and std.dev: (903.88, 1007.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8633)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676822 reads in 188.951 CPU sec, 6.091 real sec
[M::mem_process_seqs] Processed 676800 reads in 106.849 CPU sec, 3.430 real sec
sorting block 37, #reads = 1082516 mem = 536880028 peak = 1034162176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288141, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 38, #reads = 1082721 mem = 536879356 peak = 1034162176
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.60, 115.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 89, 3636)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10898)
[M::mem_pestat] mean and std.dev: (1224.40, 1730.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14529)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676866 sequences (100000095 bp) in 2.230 CPU sec, 2.447 real sec...
[M::process] read 676700 sequences (100000004 bp) in 1.500 CPU sec, 1.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287975, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.53, 113.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 29, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (98.20, 147.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676582 reads in 182.916 CPU sec, 5.933 real sec
[M::mem_process_seqs] Processed 676770 reads in 102.203 CPU sec, 3.358 real sec
sorting block 39, #reads = 1082315 mem = 536879004 peak = 1034162176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287818, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.71, 115.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 73, 91)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239)
[M::mem_pestat] mean and std.dev: (54.18, 41.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 313)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676670 sequences (100000150 bp) in 2.027 CPU sec, 2.261 real sec...
[M::process] read 676372 sequences (100000121 bp) in 1.456 CPU sec, 1.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287961, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.23, 115.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676866 reads in 188.227 CPU sec, 6.068 real sec
[M::mem_process_seqs] Processed 676700 reads in 106.441 CPU sec, 3.447 real sec
sorting block 40, #reads = 1081840 mem = 536878075 peak = 1034162176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288036, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.05, 114.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (684, 3636, 4258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11406)
[M::mem_pestat] mean and std.dev: (2569.50, 1725.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14980)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676170 sequences (100000005 bp) in 2.280 CPU sec, 2.567 real sec...
[M::process] read 676336 sequences (100000006 bp) in 1.562 CPU sec, 1.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286890, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.41, 119.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676670 reads in 191.215 CPU sec, 6.195 real sec
[M::mem_process_seqs] Processed 676372 reads in 108.449 CPU sec, 3.482 real sec
sorting block 41, #reads = 1081736 mem = 536878800 peak = 1034162176
[M::process] read 676820 sequences (100000046 bp) in 1.819 CPU sec, 1.954 real sec...
[M::process] read 677176 sequences (100000167 bp) in 1.561 CPU sec, 1.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287000, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.00, 120.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676336 reads in 103.851 CPU sec, 3.337 real sec
sorting block 42, #reads = 1081846 mem = 536877906 peak = 1034162176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286548, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (242.83, 124.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 89, 1537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4559)
[M::mem_pestat] mean and std.dev: (346.82, 457.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6070)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676976 sequences (100000091 bp) in 1.552 CPU sec, 1.704 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287482, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.79, 112.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676170 reads in 297.149 CPU sec, 9.572 real sec
[M::mem_process_seqs] Processed 676820 reads in 210.601 CPU sec, 6.775 real sec
sorting block 43, #reads = 1081533 mem = 536878981 peak = 1034162176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288198, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.97, 102.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 203, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (141.50, 124.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1329)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676756 sequences (100000183 bp) in 2.317 CPU sec, 2.731 real sec...
[M::process] read 676782 sequences (100000106 bp) in 1.318 CPU sec, 1.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287191, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.17, 110.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 91, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144)
[M::mem_pestat] mean and std.dev: (203.10, 209.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1523)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677176 reads in 193.767 CPU sec, 6.280 real sec
[M::mem_process_seqs] Processed 676976 reads in 108.304 CPU sec, 3.480 real sec
sorting block 44, #reads = 1082223 mem = 536879714 peak = 1034162176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287942, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.85, 112.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676936 sequences (100000001 bp) in 2.112 CPU sec, 2.432 real sec...
[M::process] read 676910 sequences (100000141 bp) in 1.474 CPU sec, 1.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287573, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.55, 113.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676756 reads in 192.371 CPU sec, 6.199 real sec
sorting block 45, #reads = 1082717 mem = 536878126 peak = 1037991936
[M::mem_process_seqs] Processed 676782 reads in 107.264 CPU sec, 3.446 real sec
sorting block 46, #reads = 1082411 mem = 536880228 peak = 1060446208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287742, 14, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (227.93, 111.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 125, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1899)
[M::mem_pestat] mean and std.dev: (290.46, 372.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2528)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677030 sequences (100000217 bp) in 2.360 CPU sec, 2.614 real sec...
[M::process] read 677036 sequences (100000044 bp) in 1.581 CPU sec, 1.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287664, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.32, 110.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676936 reads in 188.177 CPU sec, 6.062 real sec
[M::mem_process_seqs] Processed 676910 reads in 106.918 CPU sec, 3.425 real sec
sorting block 47, #reads = 1081615 mem = 536878850 peak = 1060446208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287484, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.53, 110.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 337, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1665)
[M::mem_pestat] mean and std.dev: (305.70, 243.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2207)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676826 sequences (100000033 bp) in 1.819 CPU sec, 2.100 real sec...
[M::process] read 676434 sequences (100000122 bp) in 1.559 CPU sec, 1.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287684, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.16, 109.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677030 reads in 189.644 CPU sec, 6.125 real sec
[M::mem_process_seqs] Processed 677036 reads in 105.682 CPU sec, 3.399 real sec
sorting block 48, #reads = 1082069 mem = 536878866 peak = 1060446208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287511, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.15, 111.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676390 sequences (100000203 bp) in 2.057 CPU sec, 2.552 real sec...
[M::process] read 676440 sequences (100000087 bp) in 1.398 CPU sec, 1.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287327, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.05, 117.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 581, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1711)
[M::mem_pestat] mean and std.dev: (386.50, 394.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2276)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676826 reads in 190.673 CPU sec, 6.212 real sec
[M::mem_process_seqs] Processed 676434 reads in 110.154 CPU sec, 3.567 real sec
sorting block 49, #reads = 1082334 mem = 536878477 peak = 1060446208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286612, 20, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 50, #reads = 1081507 mem = 536880146 peak = 1060446208
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.55, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 39, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139)
[M::mem_pestat] mean and std.dev: (217.90, 297.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1517)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677196 sequences (100000256 bp) in 2.122 CPU sec, 2.420 real sec...
[M::process] read 676890 sequences (100000195 bp) in 1.489 CPU sec, 1.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287084, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.68, 117.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676390 reads in 199.639 CPU sec, 6.439 real sec
[M::mem_process_seqs] Processed 676440 reads in 110.933 CPU sec, 3.569 real sec
sorting block 51, #reads = 1081709 mem = 536876188 peak = 1060446208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287558, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.19, 102.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676694 sequences (100000139 bp) in 2.239 CPU sec, 2.516 real sec...
[M::process] read 676762 sequences (100000266 bp) in 1.529 CPU sec, 1.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287775, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.64, 110.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677196 reads in 192.289 CPU sec, 6.203 real sec
[M::mem_process_seqs] Processed 676890 reads in 107.228 CPU sec, 3.440 real sec
sorting block 52, #reads = 1082530 mem = 536879808 peak = 1060446208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287368, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.71, 111.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676714 sequences (100000150 bp) in 1.976 CPU sec, 2.310 real sec...
[M::process] read 677280 sequences (100000028 bp) in 1.634 CPU sec, 1.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287711, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.63, 114.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (424, 428, 735)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1357)
[M::mem_pestat] mean and std.dev: (480.58, 218.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1668)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676694 reads in 191.892 CPU sec, 6.192 real sec
[M::mem_process_seqs] Processed 676762 reads in 108.354 CPU sec, 3.466 real sec
sorting block 53, #reads = 1082548 mem = 536879745 peak = 1060446208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288041, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.91, 115.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 208, 3108)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8908)
[M::mem_pestat] mean and std.dev: (1120.62, 1331.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11808)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677238 sequences (100000006 bp) in 2.467 CPU sec, 2.690 real sec...
[M::process] read 677146 sequences (100000030 bp) in 1.647 CPU sec, 1.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287921, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.41, 108.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676714 reads in 182.114 CPU sec, 5.878 real sec
[M::mem_process_seqs] Processed 677280 reads in 102.658 CPU sec, 3.330 real sec
sorting block 54, #reads = 1082376 mem = 536878255 peak = 1060446208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288283, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.21, 106.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1081162 mem = 536878368 peak = 1060610048
[M::process] read 677078 sequences (100000104 bp) in 2.110 CPU sec, 2.487 real sec...
[M::process] read 676882 sequences (100000004 bp) in 1.556 CPU sec, 1.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287946, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.59, 105.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677238 reads in 185.868 CPU sec, 6.000 real sec
[M::mem_process_seqs] Processed 677146 reads in 105.659 CPU sec, 3.372 real sec
sorting block 56, #reads = 1082072 mem = 536880289 peak = 1060610048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287446, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.57, 108.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676702 sequences (100000091 bp) in 2.284 CPU sec, 2.498 real sec...
[M::process] read 676802 sequences (100000275 bp) in 1.695 CPU sec, 1.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287684, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.44, 111.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677078 reads in 191.054 CPU sec, 6.145 real sec
[M::mem_process_seqs] Processed 676882 reads in 107.870 CPU sec, 3.458 real sec
sorting block 57, #reads = 1081351 mem = 536877084 peak = 1060610048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287478, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.67, 114.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676940 sequences (100000041 bp) in 1.858 CPU sec, 2.352 real sec...
[M::process] read 677024 sequences (100000169 bp) in 1.423 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287545, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.88, 114.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 119, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (139.25, 116.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676702 reads in 194.409 CPU sec, 6.310 real sec
[M::mem_process_seqs] Processed 676802 reads in 110.076 CPU sec, 3.544 real sec
sorting block 58, #reads = 1081864 mem = 536880351 peak = 1060610048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288100, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.67, 107.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676742 sequences (100000076 bp) in 1.956 CPU sec, 2.259 real sec...
[M::process] read 676582 sequences (100000186 bp) in 1.374 CPU sec, 1.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288210, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.74, 107.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676940 reads in 187.580 CPU sec, 6.079 real sec
[M::mem_process_seqs] Processed 677024 reads in 105.681 CPU sec, 3.388 real sec
sorting block 59, #reads = 1081938 mem = 536881596 peak = 1060610048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287974, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.77, 113.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1081907 mem = 536878752 peak = 1065902080
[M::process] read 676426 sequences (100000003 bp) in 2.136 CPU sec, 2.420 real sec...
[M::process] read 556448 sequences (82234712 bp) in 1.364 CPU sec, 1.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287723, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.85, 115.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 355, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1807)
[M::mem_pestat] mean and std.dev: (334.91, 305.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2383)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676742 reads in 188.663 CPU sec, 6.075 real sec
[M::mem_process_seqs] Processed 676582 reads in 106.658 CPU sec, 3.414 real sec
sorting block 61, #reads = 1082128 mem = 536877568 peak = 1065902080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 236235, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.19, 117.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 556448 reads in 80.234 CPU sec, 2.634 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287482, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.02, 117.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676426 reads in 181.183 CPU sec, 6.526 real sec
sorting block 62, #reads = 1082116 mem = 536879582 peak = 1065902080
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 68217484
overall: 69783760896 mem 11444.515 user 388.290 sys 396.872 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L004\tPU:L004\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.T21-D0003376_R1.fastq.gz 0002.T21-D0003376_R2.fastq.gz
[main] Real time: 396.907 sec; CPU: 11832.807 sec
sorting block 63, #reads = 568602 mem = 282093107 peak = 1065902080
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2648752480
output reads: 68741891
bam_mem_sort: 64 calls 40.637 user 0.687 sys 42.371 real
bam_write: 64 calls 34.190 user 2.545 sys 68.003 real
execute: 1 calls 18.149 user 1.537 sys 418.367 real
merge_files: 1 calls 18.103 user 1.537 sys 20.957 real
parse_chunk: 1017 calls 46.009 user 12.257 sys 70.526 real
read_chunk: 2738 calls 21.646 user 12.821 sys 330.620 real
sort_block: 1 calls 15.802 user 12.155 sys 322.073 real
write_chunk: 1719 calls 17.672 user 1.396 sys 20.045 real
overall: 2481963008 mem 680.707 user 45.620 sys 418.391 real
ls: cannot access '*.cram': No such file or directory