.command.err - Workdir - DAQ:SENTIEON_BWAMEM (T21-D0003376;L007;0002)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cd/5dc7e625fad27edf715d66df5ed0cd/.command.err
Size: 87.2 KB
Task: DAQ:SENTIEON_BWAMEM (T21-D0003376;L007;0002)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2841.720 CPU sec, 111.277 real sec
[M::process] read 677654 sequences (100000189 bp) in 1.317 CPU sec, 1.317 real sec...
[M::process] read 677482 sequences (100000015 bp) in 1.574 CPU sec, 1.616 real sec...
[M::process] read 677128 sequences (100000125 bp) in 1.565 CPU sec, 1.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289081, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (216.19, 94.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 678)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677134 sequences (100000241 bp) in 1.599 CPU sec, 1.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288531, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.67, 99.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288851, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (223.15, 103.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677654 reads in 337.108 CPU sec, 11.180 real sec
[M::mem_process_seqs] Processed 677482 reads in 240.712 CPU sec, 8.130 real sec
[M::mem_process_seqs] Processed 677128 reads in 255.216 CPU sec, 8.681 real sec
sorting block 0, #reads = 1082873 mem = 536878324 peak = 572030976
[M::process] read 676988 sequences (100000198 bp) in 2.939 CPU sec, 3.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288315, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.65, 104.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676774 sequences (100000053 bp) in 1.675 CPU sec, 1.772 real sec...
[M::process] read 676726 sequences (100000050 bp) in 1.146 CPU sec, 1.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288115, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (225.25, 105.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677134 reads in 222.172 CPU sec, 7.177 real sec
sorting block 1, #reads = 1082491 mem = 536877407 peak = 697860096
[M::mem_process_seqs] Processed 676988 reads in 131.748 CPU sec, 4.283 real sec
sorting block 2, #reads = 1082843 mem = 536877376 peak = 817537024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288083, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.82, 109.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676664 sequences (100000230 bp) in 3.278 CPU sec, 3.607 real sec...
[M::process] read 676660 sequences (100000193 bp) in 1.480 CPU sec, 1.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287828, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.94, 109.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676774 reads in 246.449 CPU sec, 7.963 real sec
[M::process] read 676512 sequences (100000068 bp) in 1.670 CPU sec, 1.670 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288099, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.98, 111.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676664 reads in 131.646 CPU sec, 4.272 real sec
sorting block 3, #reads = 1082340 mem = 536880846 peak = 817537024
[M::mem_process_seqs] Processed 676726 reads in 322.855 CPU sec, 10.503 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288053, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (232.38, 112.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 24, 826)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2472)
[M::mem_pestat] mean and std.dev: (281.40, 396.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3295)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 4, #reads = 1082482 mem = 536881004 peak = 817537024
[M::process] read 676470 sequences (100000105 bp) in 2.892 CPU sec, 3.264 real sec...
[M::process] read 676230 sequences (100000242 bp) in 1.370 CPU sec, 1.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288182, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.32, 114.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676660 reads in 260.481 CPU sec, 8.459 real sec
[M::mem_process_seqs] Processed 676512 reads in 135.706 CPU sec, 4.444 real sec
sorting block 5, #reads = 1082508 mem = 536880773 peak = 817537024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287856, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.09, 115.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676622 sequences (100000040 bp) in 3.520 CPU sec, 3.900 real sec...
[M::process] read 677530 sequences (100000257 bp) in 1.669 CPU sec, 1.671 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287738, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.52, 119.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676470 reads in 263.401 CPU sec, 8.541 real sec
[M::process] read 677390 sequences (100000179 bp) in 1.506 CPU sec, 1.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288006, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.93, 113.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676622 reads in 127.437 CPU sec, 4.142 real sec
sorting block 6, #reads = 1082160 mem = 536879542 peak = 817537024
[M::mem_process_seqs] Processed 676230 reads in 328.218 CPU sec, 10.695 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289153, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.52, 98.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1082390 mem = 536879133 peak = 817537024
[M::process] read 677180 sequences (100000064 bp) in 2.623 CPU sec, 3.080 real sec...
[M::process] read 676960 sequences (100000184 bp) in 1.607 CPU sec, 1.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288517, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.29, 101.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 113, 924)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2546)
[M::mem_pestat] mean and std.dev: (552.55, 803.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3768)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677530 reads in 238.910 CPU sec, 7.826 real sec
[M::mem_process_seqs] Processed 677390 reads in 128.450 CPU sec, 4.269 real sec
sorting block 8, #reads = 1082611 mem = 536879185 peak = 817537024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288538, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.96, 104.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676946 sequences (100000173 bp) in 3.055 CPU sec, 3.399 real sec...
[M::process] read 676942 sequences (100000134 bp) in 1.469 CPU sec, 1.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288055, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.47, 106.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677180 reads in 221.300 CPU sec, 7.097 real sec
[M::mem_process_seqs] Processed 676960 reads in 124.134 CPU sec, 3.993 real sec
sorting block 9, #reads = 1082361 mem = 536879164 peak = 817537024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288499, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.64, 109.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676820 sequences (100000047 bp) in 3.075 CPU sec, 3.406 real sec...
[M::process] read 676614 sequences (100000088 bp) in 1.820 CPU sec, 1.824 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287957, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.99, 111.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676946 reads in 222.070 CPU sec, 7.130 real sec
[M::mem_process_seqs] Processed 676942 reads in 126.328 CPU sec, 4.051 real sec
sorting block 10, #reads = 1082248 mem = 536878416 peak = 817537024
[M::process] read 676434 sequences (100000082 bp) in 1.790 CPU sec, 1.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287947, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.66, 110.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676542 sequences (100000033 bp) in 1.622 CPU sec, 1.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288277, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.82, 112.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287783, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.13, 112.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676820 reads in 322.872 CPU sec, 10.419 real sec
sorting block 11, #reads = 1082399 mem = 536875846 peak = 817537024
[M::mem_process_seqs] Processed 676614 reads in 224.581 CPU sec, 7.287 real sec
[M::mem_process_seqs] Processed 676434 reads in 234.456 CPU sec, 7.649 real sec
sorting block 12, #reads = 1082152 mem = 536879983 peak = 817537024
[M::process] read 676502 sequences (100000200 bp) in 2.890 CPU sec, 3.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287870, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.42, 115.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676148 sequences (100000022 bp) in 1.723 CPU sec, 1.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288047, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.55, 116.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677272 sequences (100000186 bp) in 1.740 CPU sec, 1.744 real sec...
[M::mem_process_seqs] Processed 676542 reads in 221.453 CPU sec, 7.108 real sec
sorting block 13, #reads = 1082097 mem = 536879953 peak = 817537024
[M::mem_process_seqs] Processed 676502 reads in 123.900 CPU sec, 3.958 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287866, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.13, 118.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1082424 mem = 536878597 peak = 857964544
[M::process] read 677622 sequences (100000041 bp) in 3.013 CPU sec, 3.439 real sec...
[M::process] read 677346 sequences (100000138 bp) in 1.729 CPU sec, 1.728 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288420, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.01, 107.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676148 reads in 218.707 CPU sec, 7.005 real sec
[M::mem_process_seqs] Processed 677272 reads in 124.860 CPU sec, 4.018 real sec
sorting block 15, #reads = 1081951 mem = 536877951 peak = 857964544
[M::process] read 677160 sequences (100000277 bp) in 2.261 CPU sec, 2.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288884, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.50, 100.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677120 sequences (100000148 bp) in 1.531 CPU sec, 1.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288679, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.72, 105.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 92, 662)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1854)
[M::mem_pestat] mean and std.dev: (161.75, 194.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2450)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288765, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.35, 106.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677622 reads in 309.459 CPU sec, 9.987 real sec
[M::mem_process_seqs] Processed 677346 reads in 215.053 CPU sec, 7.106 real sec
[M::mem_process_seqs] Processed 677160 reads in 224.622 CPU sec, 7.480 real sec
sorting block 16, #reads = 1083080 mem = 536880603 peak = 857964544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288330, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.32, 106.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676830 sequences (100000295 bp) in 3.398 CPU sec, 3.794 real sec...
[M::mem_process_seqs] Processed 677120 reads in 115.052 CPU sec, 3.813 real sec
sorting block 17, #reads = 1082746 mem = 536880098 peak = 857964544
[M::process] read 676718 sequences (100000234 bp) in 2.606 CPU sec, 2.890 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288253, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.46, 109.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 26, 606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1782)
[M::mem_pestat] mean and std.dev: (220.18, 331.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2370)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676754 sequences (100000063 bp) in 2.034 CPU sec, 2.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288622, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.08, 111.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 41, 76)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184)
[M::mem_pestat] mean and std.dev: (43.08, 41.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 238)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676502 sequences (100000094 bp) in 1.660 CPU sec, 1.667 real sec...
[M::mem_process_seqs] Processed 676830 reads in 217.525 CPU sec, 6.937 real sec
sorting block 18, #reads = 1082379 mem = 536877572 peak = 857964544
[M::mem_process_seqs] Processed 676718 reads in 122.166 CPU sec, 3.891 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288082, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.61, 112.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1082441 mem = 536881340 peak = 894963712
[M::process] read 676516 sequences (100000054 bp) in 4.015 CPU sec, 4.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287819, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.97, 114.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676420 sequences (100000088 bp) in 1.861 CPU sec, 1.864 real sec...
[M::mem_process_seqs] Processed 676754 reads in 221.553 CPU sec, 7.077 real sec
[M::mem_process_seqs] Processed 676502 reads in 127.822 CPU sec, 4.093 real sec
sorting block 20, #reads = 1082069 mem = 536877957 peak = 894963712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287932, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.42, 115.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676176 sequences (100000252 bp) in 2.602 CPU sec, 2.878 real sec...
[M::process] read 676206 sequences (100000276 bp) in 1.902 CPU sec, 1.904 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287635, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.84, 115.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (49, 1075, 1660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4882)
[M::mem_pestat] mean and std.dev: (930.91, 761.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6493)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676516 reads in 227.520 CPU sec, 7.313 real sec
[M::mem_process_seqs] Processed 676420 reads in 124.588 CPU sec, 3.986 real sec
sorting block 21, #reads = 1081974 mem = 536877712 peak = 894963712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288100, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.52, 119.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677522 sequences (100000070 bp) in 2.774 CPU sec, 2.988 real sec...
[M::process] read 677334 sequences (100000257 bp) in 1.523 CPU sec, 1.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287747, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.02, 118.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676176 reads in 218.886 CPU sec, 7.018 real sec
[M::mem_process_seqs] Processed 676206 reads in 118.816 CPU sec, 3.803 real sec
sorting block 22, #reads = 1082053 mem = 536879508 peak = 894963712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288522, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.98, 98.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677252 sequences (100000267 bp) in 2.528 CPU sec, 2.789 real sec...
[M::process] read 677046 sequences (100000265 bp) in 1.666 CPU sec, 1.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288189, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.86, 101.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677522 reads in 219.863 CPU sec, 7.052 real sec
sorting block 23, #reads = 1081365 mem = 536879721 peak = 894963712
[M::mem_process_seqs] Processed 677334 reads in 118.762 CPU sec, 3.802 real sec
sorting block 24, #reads = 1082639 mem = 536879964 peak = 903782400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287985, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.94, 102.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676928 sequences (100000040 bp) in 3.042 CPU sec, 3.299 real sec...
[M::process] read 676838 sequences (100000205 bp) in 1.940 CPU sec, 1.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288709, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.95, 105.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 73, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 201)
[M::mem_pestat] mean and std.dev: (40.12, 32.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 265)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677252 reads in 227.222 CPU sec, 7.284 real sec
[M::mem_process_seqs] Processed 677046 reads in 128.105 CPU sec, 4.092 real sec
sorting block 25, #reads = 1082676 mem = 536882199 peak = 903782400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288202, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.50, 108.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676840 sequences (100000125 bp) in 2.649 CPU sec, 2.890 real sec...
[M::process] read 676592 sequences (100000209 bp) in 1.983 CPU sec, 1.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288501, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.62, 109.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676928 reads in 230.387 CPU sec, 7.398 real sec
[M::mem_process_seqs] Processed 676838 reads in 127.200 CPU sec, 4.054 real sec
sorting block 26, #reads = 1082537 mem = 536878935 peak = 903782400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288282, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.03, 111.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676532 sequences (100000111 bp) in 3.013 CPU sec, 3.274 real sec...
[M::process] read 676388 sequences (100000058 bp) in 1.700 CPU sec, 1.705 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288417, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.73, 112.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676840 reads in 228.944 CPU sec, 7.345 real sec
[M::mem_process_seqs] Processed 676592 reads in 126.647 CPU sec, 4.041 real sec
sorting block 27, #reads = 1082396 mem = 536881564 peak = 903782400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287898, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.63, 114.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676316 sequences (100000060 bp) in 2.723 CPU sec, 2.996 real sec...
[M::process] read 676184 sequences (100000064 bp) in 1.752 CPU sec, 1.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287699, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.59, 115.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676532 reads in 230.775 CPU sec, 7.407 real sec
sorting block 28, #reads = 1082614 mem = 536878658 peak = 903782400
[M::mem_process_seqs] Processed 676388 reads in 126.654 CPU sec, 4.047 real sec
sorting block 29, #reads = 1082439 mem = 536878055 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287658, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.17, 117.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676496 sequences (100000018 bp) in 3.076 CPU sec, 3.351 real sec...
[M::process] read 677756 sequences (100000175 bp) in 1.792 CPU sec, 1.794 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287879, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.99, 119.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676316 reads in 226.449 CPU sec, 7.266 real sec
[M::mem_process_seqs] Processed 676184 reads in 125.762 CPU sec, 4.014 real sec
sorting block 30, #reads = 1082039 mem = 536881345 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288120, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.11, 114.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677598 sequences (100000075 bp) in 2.897 CPU sec, 3.179 real sec...
[M::process] read 677608 sequences (100000104 bp) in 1.846 CPU sec, 1.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288579, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515)
[M::mem_pestat] mean and std.dev: (210.24, 87.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 633)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 107, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1415)
[M::mem_pestat] mean and std.dev: (293.70, 416.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1959)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676496 reads in 221.671 CPU sec, 7.089 real sec
[M::mem_process_seqs] Processed 677756 reads in 125.760 CPU sec, 4.014 real sec
sorting block 31, #reads = 1081759 mem = 536877662 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288177, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (213.23, 91.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 658)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 44, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (148.57, 153.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677410 sequences (100000108 bp) in 3.163 CPU sec, 3.434 real sec...
[M::process] read 677206 sequences (100000278 bp) in 1.901 CPU sec, 1.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288273, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (218.91, 99.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677598 reads in 231.526 CPU sec, 7.423 real sec
[M::mem_process_seqs] Processed 677608 reads in 127.861 CPU sec, 4.084 real sec
sorting block 32, #reads = 1083064 mem = 536880176 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288318, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.52, 99.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1082197 mem = 536878822 peak = 944050176
[M::process] read 677308 sequences (100000075 bp) in 2.692 CPU sec, 2.963 real sec...
[M::process] read 677056 sequences (100000143 bp) in 1.728 CPU sec, 1.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288243, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.50, 104.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677410 reads in 232.729 CPU sec, 7.474 real sec
[M::mem_process_seqs] Processed 677206 reads in 128.993 CPU sec, 4.133 real sec
sorting block 34, #reads = 1082939 mem = 536881015 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288409, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.78, 108.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 1, 38)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 112)
[M::mem_pestat] mean and std.dev: (7.33, 12.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 149)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676818 sequences (100000119 bp) in 3.304 CPU sec, 3.613 real sec...
[M::process] read 676760 sequences (100000248 bp) in 1.925 CPU sec, 1.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287986, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.80, 109.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677308 reads in 235.262 CPU sec, 7.524 real sec
[M::mem_process_seqs] Processed 677056 reads in 129.113 CPU sec, 4.109 real sec
sorting block 35, #reads = 1083251 mem = 536881491 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288146, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.71, 110.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676762 sequences (100000100 bp) in 3.000 CPU sec, 3.278 real sec...
[M::process] read 676728 sequences (100000003 bp) in 1.984 CPU sec, 1.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287427, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.42, 111.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 76, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (124.50, 130.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676818 reads in 236.062 CPU sec, 7.575 real sec
[M::mem_process_seqs] Processed 676760 reads in 132.476 CPU sec, 4.233 real sec
sorting block 36, #reads = 1082876 mem = 536878211 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287939, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.09, 112.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676596 sequences (100000233 bp) in 3.283 CPU sec, 3.575 real sec...
[M::process] read 677694 sequences (100000138 bp) in 2.072 CPU sec, 2.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287937, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.89, 114.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676762 reads in 239.067 CPU sec, 7.653 real sec
[M::mem_process_seqs] Processed 676728 reads in 132.732 CPU sec, 4.234 real sec
sorting block 37, #reads = 1082552 mem = 536880362 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287815, 14, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.76, 116.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 182, 743)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2127)
[M::mem_pestat] mean and std.dev: (440.57, 433.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2819)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 38, #reads = 1082334 mem = 536876381 peak = 944050176
[M::process] read 677844 sequences (100000168 bp) in 3.088 CPU sec, 3.361 real sec...
[M::process] read 677574 sequences (100000286 bp) in 2.115 CPU sec, 2.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288947, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (209.58, 87.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676596 reads in 239.051 CPU sec, 7.671 real sec
[M::mem_process_seqs] Processed 677694 reads in 131.545 CPU sec, 4.196 real sec
sorting block 39, #reads = 1082654 mem = 536878421 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289013, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (210.68, 88.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677582 sequences (100000108 bp) in 3.427 CPU sec, 3.729 real sec...
[M::process] read 677262 sequences (100000115 bp) in 2.164 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288296, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558)
[M::mem_pestat] mean and std.dev: (216.92, 97.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677844 reads in 236.441 CPU sec, 7.583 real sec
[M::mem_process_seqs] Processed 677574 reads in 131.566 CPU sec, 4.192 real sec
sorting block 40, #reads = 1082978 mem = 536881591 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288327, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.41, 99.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 9, 66)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 192)
[M::mem_pestat] mean and std.dev: (33.86, 38.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 255)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677204 sequences (100000196 bp) in 2.717 CPU sec, 2.979 real sec...
[M::process] read 677006 sequences (100000181 bp) in 1.790 CPU sec, 1.791 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288148, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.74, 103.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677582 reads in 239.588 CPU sec, 7.714 real sec
[M::mem_process_seqs] Processed 677262 reads in 131.985 CPU sec, 4.225 real sec
sorting block 41, #reads = 1082699 mem = 536878820 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288228, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.42, 106.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677074 sequences (100000054 bp) in 3.321 CPU sec, 3.621 real sec...
[M::process] read 676800 sequences (100000071 bp) in 2.093 CPU sec, 2.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287766, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.08, 108.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677204 reads in 241.391 CPU sec, 7.736 real sec
[M::mem_process_seqs] Processed 677006 reads in 132.143 CPU sec, 4.209 real sec
sorting block 42, #reads = 1082082 mem = 536879001 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288502, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.51, 110.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 43, #reads = 1082532 mem = 536883167 peak = 944050176
[M::process] read 676766 sequences (100000173 bp) in 2.903 CPU sec, 3.216 real sec...
[M::process] read 676800 sequences (100000294 bp) in 2.018 CPU sec, 2.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287720, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.95, 111.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677074 reads in 233.449 CPU sec, 7.486 real sec
[M::mem_process_seqs] Processed 676800 reads in 130.368 CPU sec, 4.167 real sec
sorting block 44, #reads = 1082393 mem = 536879367 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287581, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.21, 112.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 92, 713)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1997)
[M::mem_pestat] mean and std.dev: (232.22, 294.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2639)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676736 sequences (100000268 bp) in 3.812 CPU sec, 4.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288139, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (227.88, 111.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676766 reads in 236.480 CPU sec, 7.588 real sec
[M::process] read 677606 sequences (100000160 bp) in 2.243 CPU sec, 2.264 real sec...
[M::mem_process_seqs] Processed 676800 reads in 133.891 CPU sec, 4.268 real sec
sorting block 45, #reads = 1082223 mem = 536878701 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287919, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.49, 113.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677760 sequences (100000290 bp) in 2.924 CPU sec, 3.323 real sec...
[M::process] read 677616 sequences (100000166 bp) in 2.031 CPU sec, 2.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288938, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.10, 99.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676736 reads in 228.378 CPU sec, 7.346 real sec
[M::mem_process_seqs] Processed 677606 reads in 127.441 CPU sec, 4.059 real sec
sorting block 46, #reads = 1082646 mem = 536879404 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288472, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (212.51, 91.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 658)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 274, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (187.00, 100.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677576 sequences (100000163 bp) in 3.537 CPU sec, 3.924 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288480, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.27, 99.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677322 sequences (100000244 bp) in 1.887 CPU sec, 1.895 real sec...
[M::mem_process_seqs] Processed 677760 reads in 230.024 CPU sec, 7.394 real sec
[M::mem_process_seqs] Processed 677616 reads in 131.009 CPU sec, 4.182 real sec
sorting block 47, #reads = 1082648 mem = 536877491 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288144, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.77, 102.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1082569 mem = 536878878 peak = 944050176
[M::process] read 677244 sequences (100000006 bp) in 3.036 CPU sec, 3.398 real sec...
[M::process] read 677020 sequences (100000218 bp) in 1.800 CPU sec, 1.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288568, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.17, 102.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677576 reads in 223.598 CPU sec, 7.187 real sec
[M::mem_process_seqs] Processed 677322 reads in 122.431 CPU sec, 3.887 real sec
sorting block 49, #reads = 1082743 mem = 536879361 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287760, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.16, 107.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676952 sequences (100000089 bp) in 3.317 CPU sec, 3.610 real sec...
[M::process] read 676982 sequences (100000295 bp) in 2.091 CPU sec, 2.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288296, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.27, 109.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677244 reads in 215.148 CPU sec, 6.855 real sec
[M::mem_process_seqs] Processed 677020 reads in 119.723 CPU sec, 3.795 real sec
sorting block 50, #reads = 1082703 mem = 536879745 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288241, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.76, 110.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676822 sequences (100000038 bp) in 3.011 CPU sec, 3.331 real sec...
[M::process] read 676848 sequences (100000236 bp) in 2.059 CPU sec, 2.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288130, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.63, 111.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 39, 1974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5898)
[M::mem_pestat] mean and std.dev: (557.42, 829.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7860)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676952 reads in 226.074 CPU sec, 7.247 real sec
[M::mem_process_seqs] Processed 676982 reads in 127.931 CPU sec, 4.077 real sec
sorting block 51, #reads = 1082935 mem = 536878707 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288296, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.77, 112.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676702 sequences (100000050 bp) in 3.380 CPU sec, 3.713 real sec...
[M::process] read 677248 sequences (100000087 bp) in 1.996 CPU sec, 2.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288297, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.95, 113.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676822 reads in 226.921 CPU sec, 7.269 real sec
sorting block 52, #reads = 1082998 mem = 536880104 peak = 944050176
[M::mem_process_seqs] Processed 676848 reads in 128.403 CPU sec, 4.084 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287943, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (231.82, 115.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1082817 mem = 536877458 peak = 944050176
[M::process] read 677600 sequences (100000020 bp) in 2.900 CPU sec, 3.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288189, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.07, 107.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676702 reads in 226.332 CPU sec, 7.275 real sec
[M::process] read 677564 sequences (100000175 bp) in 2.281 CPU sec, 2.304 real sec...
[M::mem_process_seqs] Processed 677248 reads in 127.033 CPU sec, 4.030 real sec
sorting block 54, #reads = 1082372 mem = 536879690 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288720, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (211.14, 88.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 645)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677408 sequences (100000221 bp) in 3.188 CPU sec, 3.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288495, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (216.25, 95.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677300 sequences (100000257 bp) in 2.240 CPU sec, 2.238 real sec...
[M::mem_process_seqs] Processed 677600 reads in 227.853 CPU sec, 7.313 real sec
[M::mem_process_seqs] Processed 677564 reads in 128.274 CPU sec, 4.084 real sec
sorting block 55, #reads = 1082275 mem = 536878617 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287962, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (218.12, 98.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677110 sequences (100000070 bp) in 3.051 CPU sec, 3.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288103, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.35, 103.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677408 reads in 218.456 CPU sec, 6.999 real sec
[M::process] read 677016 sequences (100000273 bp) in 2.010 CPU sec, 2.011 real sec...
[M::mem_process_seqs] Processed 677300 reads in 121.210 CPU sec, 3.838 real sec
sorting block 56, #reads = 1082669 mem = 536878758 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287968, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.49, 107.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 427, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1773)
[M::mem_pestat] mean and std.dev: (248.12, 217.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2339)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676878 sequences (100000124 bp) in 2.572 CPU sec, 2.816 real sec...
[M::process] read 676922 sequences (100000233 bp) in 1.504 CPU sec, 1.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287684, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.27, 109.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677110 reads in 210.151 CPU sec, 6.732 real sec
sorting block 57, #reads = 1082538 mem = 536880415 peak = 944050176
[M::mem_process_seqs] Processed 677016 reads in 118.840 CPU sec, 3.810 real sec
sorting block 58, #reads = 1082548 mem = 536881241 peak = 944050176
[M::process] read 676806 sequences (100000245 bp) in 2.145 CPU sec, 2.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288214, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.76, 110.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676668 sequences (100000186 bp) in 1.320 CPU sec, 1.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287797, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.79, 113.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287751, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.33, 113.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676878 reads in 306.974 CPU sec, 9.871 real sec
[M::mem_process_seqs] Processed 676922 reads in 211.183 CPU sec, 6.811 real sec
[M::mem_process_seqs] Processed 676806 reads in 219.050 CPU sec, 7.092 real sec
sorting block 59, #reads = 1082552 mem = 536880826 peak = 944050176
[M::process] read 676464 sequences (100000050 bp) in 2.423 CPU sec, 2.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287412, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.39, 115.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1082428 mem = 536876388 peak = 944050176
[M::process] read 655674 sequences (96915554 bp) in 2.530 CPU sec, 2.776 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287385, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.17, 118.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676668 reads in 222.939 CPU sec, 7.200 real sec
[M::mem_process_seqs] Processed 676464 reads in 123.246 CPU sec, 3.971 real sec
sorting block 61, #reads = 1081818 mem = 536877986 peak = 944050176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278975, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.50, 118.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 71, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 113)
[M::mem_pestat] mean and std.dev: (62.22, 16.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 134)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 655674 reads in 102.874 CPU sec, 3.421 real sec
sorting block 62, #reads = 1081952 mem = 536878813 peak = 944050176
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67677018
overall: 69337333760 mem 13799.233 user 905.703 sys 504.271 real
sorting block 63, #reads = 6512 mem = 3231365 peak = 944050176
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L007\tPU:L007\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.T21-D0003376_R1.fastq.gz 0002.T21-D0003376_R2.fastq.gz
[main] Real time: 504.320 sec; CPU: 14704.958 sec
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2645480407
output reads: 68202033
bam_mem_sort: 64 calls 42.149 user 1.469 sys 43.821 real
bam_write: 64 calls 30.604 user 2.221 sys 37.770 real
execute: 1 calls 18.493 user 1.421 sys 524.873 real
merge_files: 1 calls 18.479 user 1.377 sys 20.572 real
parse_chunk: 1009 calls 46.791 user 12.631 sys 61.097 real
read_chunk: 2716 calls 30.733 user 17.320 sys 392.387 real
sort_block: 1 calls 23.707 user 16.416 sys 380.499 real
write_chunk: 1705 calls 18.102 user 1.040 sys 19.693 real
overall: 2246172672 mem 702.872 user 53.332 sys 524.894 real
ls: cannot access '*.cram': No such file or directory