Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/51/9e963ecd4bcd25f7f3bd46532a624a/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a4/fd50031d3e07d87b6b8b7fd3253b66/T18-D0003255/0004.T18-D0003255_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a4/fd50031d3e07d87b6b8b7fd3253b66/T18-D0003255/0004.T18-D0003255_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/51/9e963ecd4bcd25f7f3bd46532a624a/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
No more license available for sentieon:klib
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1603.578 CPU sec, 532.054 real sec
[M::process] read 673952 sequences (100000128 bp) in 1.252 CPU sec, 1.256 real sec...
[M::process] read 673722 sequences (100000144 bp) in 1.970 CPU sec, 1.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286928, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (251.91, 123.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 43, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (168.15, 210.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1371)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673876 sequences (100000248 bp) in 2.000 CPU sec, 2.000 real sec...
[M::process] read 673644 sequences (100000036 bp) in 2.010 CPU sec, 2.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285963, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (254.74, 126.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 157, 689)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2009)
[M::mem_pestat] mean and std.dev: (270.09, 290.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2669)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673952 reads in 210.101 CPU sec, 6.588 real sec
[M::mem_process_seqs] Processed 673722 reads in 120.793 CPU sec, 3.808 real sec
sorting block 0, #reads = 1080319 mem = 536880190 peak = 660987904
[M::process] read 673584 sequences (100000116 bp) in 1.606 CPU sec, 1.620 real sec...
[M::process] read 673390 sequences (100000258 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286127, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.67, 127.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286097, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.46, 129.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285981, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.19, 132.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673876 reads in 314.669 CPU sec, 10.070 real sec
[M::mem_process_seqs] Processed 673644 reads in 217.385 CPU sec, 7.016 real sec
[M::mem_process_seqs] Processed 673584 reads in 227.040 CPU sec, 7.340 real sec
sorting block 1, #reads = 1080307 mem = 536876420 peak = 781705216
[M::process] read 673430 sequences (100000189 bp) in 2.093 CPU sec, 2.107 real sec...
sorting block 2, #reads = 1080474 mem = 536879621 peak = 960856064
[M::process] read 673240 sequences (100000170 bp) in 1.537 CPU sec, 1.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285304, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.44, 134.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 85, 98)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244)
[M::mem_pestat] mean and std.dev: (51.44, 36.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 317)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673198 sequences (100000252 bp) in 1.370 CPU sec, 1.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285221, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.70, 136.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 160, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367)
[M::mem_pestat] mean and std.dev: (283.43, 255.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1794)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673390 reads in 230.935 CPU sec, 7.349 real sec
[M::mem_process_seqs] Processed 673430 reads in 127.086 CPU sec, 4.058 real sec
sorting block 3, #reads = 1080070 mem = 536880745 peak = 960856064
[M::process] read 673062 sequences (100000292 bp) in 1.909 CPU sec, 1.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285626, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.37, 135.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672800 sequences (100000056 bp) in 1.360 CPU sec, 1.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285188, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.70, 139.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 23, 595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739)
[M::mem_pestat] mean and std.dev: (179.73, 254.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2311)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285037, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.45, 141.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 169, 1614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4784)
[M::mem_pestat] mean and std.dev: (570.19, 796.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6369)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673240 reads in 330.621 CPU sec, 10.588 real sec
[M::mem_process_seqs] Processed 673198 reads in 227.208 CPU sec, 7.330 real sec
[M::mem_process_seqs] Processed 673062 reads in 237.324 CPU sec, 7.672 real sec
sorting block 4, #reads = 1080327 mem = 536879017 peak = 960856064
[M::process] read 673002 sequences (100000211 bp) in 2.036 CPU sec, 2.064 real sec...
sorting block 5, #reads = 1080100 mem = 536879008 peak = 988831744
[M::process] read 672810 sequences (100000139 bp) in 1.648 CPU sec, 1.673 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284870, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.16, 144.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672728 sequences (100000112 bp) in 1.380 CPU sec, 1.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284681, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.50, 143.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 23, 105)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273)
[M::mem_pestat] mean and std.dev: (34.85, 37.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 357)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672800 reads in 234.125 CPU sec, 7.447 real sec
[M::mem_process_seqs] Processed 673002 reads in 127.128 CPU sec, 4.064 real sec
sorting block 6, #reads = 1079432 mem = 536878331 peak = 988831744
[M::process] read 672638 sequences (100000067 bp) in 2.355 CPU sec, 2.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284609, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.01, 144.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 178, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (227.00, 221.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1314)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672700 sequences (100000286 bp) in 1.930 CPU sec, 1.932 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284731, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.27, 147.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 128, 618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1812)
[M::mem_pestat] mean and std.dev: (250.58, 276.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2409)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284961, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.08, 147.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672810 reads in 333.354 CPU sec, 10.639 real sec
[M::mem_process_seqs] Processed 672638 reads in 230.136 CPU sec, 7.394 real sec
sorting block 7, #reads = 1079491 mem = 536880651 peak = 988831744
[M::mem_process_seqs] Processed 672728 reads in 240.159 CPU sec, 7.730 real sec
sorting block 8, #reads = 1079382 mem = 536877067 peak = 988831744
[M::process] read 673528 sequences (100000022 bp) in 2.935 CPU sec, 3.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284824, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 204, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (283.80, 149.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 98, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2150)
[M::mem_pestat] mean and std.dev: (324.92, 393.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2838)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673938 sequences (100000282 bp) in 1.960 CPU sec, 1.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286089, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.55, 133.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673740 sequences (100000039 bp) in 1.930 CPU sec, 1.936 real sec...
[M::mem_process_seqs] Processed 672700 reads in 226.135 CPU sec, 7.148 real sec
[M::mem_process_seqs] Processed 673528 reads in 123.299 CPU sec, 3.869 real sec
sorting block 9, #reads = 1079111 mem = 536879120 peak = 988831744
[M::process] read 673808 sequences (100000095 bp) in 2.545 CPU sec, 2.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287039, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (253.73, 125.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673660 sequences (100000156 bp) in 1.940 CPU sec, 1.942 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286544, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.56, 126.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286243, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.21, 129.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673938 reads in 309.605 CPU sec, 9.874 real sec
[M::mem_process_seqs] Processed 673740 reads in 216.044 CPU sec, 6.919 real sec
sorting block 10, #reads = 1079666 mem = 536878628 peak = 988831744
[M::mem_process_seqs] Processed 673808 reads in 225.559 CPU sec, 7.242 real sec
sorting block 11, #reads = 1080420 mem = 536877666 peak = 1043046400
[M::process] read 673486 sequences (100000268 bp) in 2.419 CPU sec, 2.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285825, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.37, 132.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673322 sequences (100000192 bp) in 1.665 CPU sec, 1.676 real sec...
[M::process] read 673216 sequences (100000064 bp) in 1.440 CPU sec, 1.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285738, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.43, 132.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 323, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024)
[M::mem_pestat] mean and std.dev: (353.00, 281.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2670)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673660 reads in 223.363 CPU sec, 7.092 real sec
[M::mem_process_seqs] Processed 673486 reads in 123.091 CPU sec, 3.906 real sec
sorting block 12, #reads = 1080647 mem = 536879439 peak = 1043046400
[M::process] read 673016 sequences (100000072 bp) in 2.443 CPU sec, 2.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285347, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.05, 136.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673024 sequences (100000166 bp) in 1.950 CPU sec, 1.949 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285687, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.09, 136.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 91, 3846)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11488)
[M::mem_pestat] mean and std.dev: (2441.64, 3926.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18148)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285662, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.80, 140.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673322 reads in 322.594 CPU sec, 10.293 real sec
[M::mem_process_seqs] Processed 673216 reads in 223.083 CPU sec, 7.147 real sec
sorting block 13, #reads = 1079822 mem = 536880398 peak = 1043046400
[M::mem_process_seqs] Processed 673016 reads in 233.012 CPU sec, 7.628 real sec
sorting block 14, #reads = 1080036 mem = 536878959 peak = 1043046400
[M::process] read 673008 sequences (100000270 bp) in 2.712 CPU sec, 2.772 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285129, 13, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.94, 142.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 621, 2178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6446)
[M::mem_pestat] mean and std.dev: (748.31, 831.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8580)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673020 sequences (100000016 bp) in 2.137 CPU sec, 2.143 real sec...
[M::process] read 672884 sequences (100000235 bp) in 1.990 CPU sec, 1.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285255, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.52, 142.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673024 reads in 228.509 CPU sec, 7.221 real sec
[M::mem_process_seqs] Processed 673008 reads in 126.504 CPU sec, 3.977 real sec
sorting block 15, #reads = 1079877 mem = 536877256 peak = 1043046400
[M::process] read 672796 sequences (100000239 bp) in 2.154 CPU sec, 2.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285312, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.54, 143.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672828 sequences (100000290 bp) in 1.410 CPU sec, 1.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284971, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.67, 148.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285459, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.59, 146.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 774, 3210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9546)
[M::mem_pestat] mean and std.dev: (1542.60, 1667.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12714)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673020 reads in 324.102 CPU sec, 10.367 real sec
sorting block 16, #reads = 1079570 mem = 536880386 peak = 1063727104
[M::mem_process_seqs] Processed 672796 reads in 225.437 CPU sec, 7.254 real sec
[M::mem_process_seqs] Processed 672884 reads in 235.324 CPU sec, 7.591 real sec
sorting block 17, #reads = 1079467 mem = 536878165 peak = 1093263360
[M::process] read 672736 sequences (100000082 bp) in 2.639 CPU sec, 2.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285410, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.21, 147.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 46, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 221)
[M::mem_pestat] mean and std.dev: (44.42, 27.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 288)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673210 sequences (100000109 bp) in 1.638 CPU sec, 1.644 real sec...
[M::process] read 673914 sequences (100000294 bp) in 1.470 CPU sec, 1.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285053, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (283.78, 150.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672828 reads in 222.261 CPU sec, 7.075 real sec
sorting block 18, #reads = 1079633 mem = 536879105 peak = 1101848576
[M::mem_process_seqs] Processed 672736 reads in 123.174 CPU sec, 3.903 real sec
sorting block 19, #reads = 1079047 mem = 536878300 peak = 1109192704
[M::process] read 673956 sequences (100000227 bp) in 2.544 CPU sec, 2.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285784, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.90, 141.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673746 sequences (100000020 bp) in 1.970 CPU sec, 1.964 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286077, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (252.95, 124.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286027, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (255.07, 126.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673210 reads in 314.598 CPU sec, 10.029 real sec
[M::mem_process_seqs] Processed 673914 reads in 217.707 CPU sec, 6.976 real sec
[M::mem_process_seqs] Processed 673956 reads in 227.193 CPU sec, 7.296 real sec
sorting block 20, #reads = 1079598 mem = 536880663 peak = 1109192704
[M::process] read 673640 sequences (100000259 bp) in 2.964 CPU sec, 3.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285786, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (256.64, 127.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673654 sequences (100000084 bp) in 1.993 CPU sec, 1.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286073, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.17, 129.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673746 reads in 222.115 CPU sec, 7.008 real sec
[M::process] read 673430 sequences (100000010 bp) in 1.990 CPU sec, 1.990 real sec...
sorting block 21, #reads = 1080296 mem = 536878282 peak = 1109192704
[M::process] read 673266 sequences (100000046 bp) in 1.890 CPU sec, 1.896 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285891, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.39, 132.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673654 reads in 119.500 CPU sec, 3.808 real sec
sorting block 22, #reads = 1079895 mem = 536880014 peak = 1109192704
[M::mem_process_seqs] Processed 673640 reads in 269.716 CPU sec, 8.541 real sec
[M::process] read 673024 sequences (100000257 bp) in 2.635 CPU sec, 2.689 real sec...
[M::process] read 673054 sequences (100000274 bp) in 1.970 CPU sec, 1.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285191, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.92, 136.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673266 reads in 115.216 CPU sec, 3.694 real sec
sorting block 23, #reads = 1079981 mem = 536878317 peak = 1121705984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284931, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.41, 134.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 198, 875)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2365)
[M::mem_pestat] mean and std.dev: (499.00, 504.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3110)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673092 sequences (100000241 bp) in 1.922 CPU sec, 1.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285366, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.56, 138.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 62, 962)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2870)
[M::mem_pestat] mean and std.dev: (371.91, 474.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3824)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673430 reads in 352.233 CPU sec, 11.214 real sec
[M::mem_process_seqs] Processed 673024 reads in 134.933 CPU sec, 4.440 real sec
sorting block 24, #reads = 1080108 mem = 536878440 peak = 1121705984
[M::process] read 672974 sequences (100000188 bp) in 2.382 CPU sec, 2.469 real sec...
[M::process] read 672786 sequences (100000156 bp) in 1.430 CPU sec, 1.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285084, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.01, 143.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673092 reads in 119.263 CPU sec, 3.835 real sec
sorting block 25, #reads = 1079635 mem = 536879314 peak = 1121705984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285008, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (273.32, 141.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672708 sequences (100000003 bp) in 1.921 CPU sec, 1.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284751, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.81, 144.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673054 reads in 350.041 CPU sec, 11.239 real sec
[M::mem_process_seqs] Processed 672974 reads in 244.085 CPU sec, 7.802 real sec
sorting block 26, #reads = 1079941 mem = 536880130 peak = 1121705984
[M::process] read 672742 sequences (100000227 bp) in 2.690 CPU sec, 2.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284677, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.07, 143.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 183, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221)
[M::mem_pestat] mean and std.dev: (251.80, 247.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1617)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672802 sequences (100000260 bp) in 2.000 CPU sec, 1.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284643, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.36, 145.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284658, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.10, 148.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 200, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739)
[M::mem_pestat] mean and std.dev: (260.80, 233.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2278)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672786 reads in 331.909 CPU sec, 10.588 real sec
[M::mem_process_seqs] Processed 672708 reads in 230.460 CPU sec, 7.379 real sec
sorting block 27, #reads = 1079555 mem = 536880337 peak = 1121705984
[M::mem_process_seqs] Processed 672742 reads in 240.370 CPU sec, 7.716 real sec
sorting block 28, #reads = 1079000 mem = 536880646 peak = 1121705984
[M::process] read 672786 sequences (100000006 bp) in 2.871 CPU sec, 2.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284697, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (283.64, 150.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 87, 117)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 177)
[M::mem_pestat] mean and std.dev: (83.88, 27.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 207)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673974 sequences (100000164 bp) in 2.110 CPU sec, 2.112 real sec...
[M::process] read 673840 sequences (100000096 bp) in 1.920 CPU sec, 1.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285366, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (282.93, 150.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 512, 4529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13503)
[M::mem_pestat] mean and std.dev: (1796.00, 2792.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17990)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672802 reads in 230.087 CPU sec, 7.269 real sec
[M::mem_process_seqs] Processed 672786 reads in 125.231 CPU sec, 3.936 real sec
sorting block 29, #reads = 1079812 mem = 536877991 peak = 1121705984
[M::process] read 673798 sequences (100000009 bp) in 2.738 CPU sec, 2.783 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286549, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (252.11, 123.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 92, 92)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 188)
[M::mem_pestat] mean and std.dev: (56.11, 34.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 236)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673682 sequences (100000228 bp) in 1.980 CPU sec, 1.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285541, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (255.12, 126.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286272, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (253.17, 124.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673974 reads in 316.602 CPU sec, 10.095 real sec
[M::mem_process_seqs] Processed 673798 reads in 222.622 CPU sec, 7.122 real sec
sorting block 30, #reads = 1079362 mem = 536878442 peak = 1121705984
[M::mem_process_seqs] Processed 673840 reads in 232.417 CPU sec, 7.561 real sec
sorting block 31, #reads = 1080319 mem = 536876294 peak = 1136922624
[M::process] read 673558 sequences (100000284 bp) in 2.376 CPU sec, 2.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285566, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (256.55, 126.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673484 sequences (100000134 bp) in 1.730 CPU sec, 1.732 real sec...
[M::process] read 673398 sequences (100000077 bp) in 1.440 CPU sec, 1.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285675, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (259.02, 129.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673682 reads in 225.808 CPU sec, 7.194 real sec
[M::mem_process_seqs] Processed 673558 reads in 123.456 CPU sec, 3.919 real sec
sorting block 32, #reads = 1080202 mem = 536879525 peak = 1169301504
[M::process] read 673186 sequences (100000083 bp) in 2.808 CPU sec, 2.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285245, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (261.22, 130.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673072 sequences (100000271 bp) in 1.990 CPU sec, 1.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284828, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.73, 134.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284918, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (268.33, 136.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 30, 164)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464)
[M::mem_pestat] mean and std.dev: (82.60, 96.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 614)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673484 reads in 333.823 CPU sec, 10.650 real sec
sorting block 33, #reads = 1080328 mem = 536880347 peak = 1169301504
[M::mem_process_seqs] Processed 673398 reads in 231.672 CPU sec, 7.424 real sec
[M::mem_process_seqs] Processed 673186 reads in 241.797 CPU sec, 7.773 real sec
sorting block 34, #reads = 1079360 mem = 536878831 peak = 1185927168
[M::process] read 673118 sequences (100000013 bp) in 2.951 CPU sec, 3.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284690, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.33, 138.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672958 sequences (100000071 bp) in 2.150 CPU sec, 2.143 real sec...
[M::process] read 672920 sequences (100000162 bp) in 1.990 CPU sec, 1.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284265, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.84, 139.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673072 reads in 241.428 CPU sec, 7.642 real sec
sorting block 35, #reads = 1079623 mem = 536879976 peak = 1185927168
[M::mem_process_seqs] Processed 673118 reads in 134.978 CPU sec, 4.254 real sec
sorting block 36, #reads = 1079708 mem = 536880142 peak = 1197264896
[M::process] read 672766 sequences (100000044 bp) in 2.092 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284664, 13, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.83, 142.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 8, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254)
[M::mem_pestat] mean and std.dev: (20.92, 31.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 336)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672806 sequences (100000066 bp) in 1.510 CPU sec, 1.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284531, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.95, 143.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 463, 620)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1818)
[M::mem_pestat] mean and std.dev: (295.92, 282.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2417)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284156, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.23, 143.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 92, 138)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (71.80, 54.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 498)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672958 reads in 342.722 CPU sec, 10.968 real sec
[M::mem_process_seqs] Processed 672920 reads in 235.679 CPU sec, 7.596 real sec
[M::mem_process_seqs] Processed 672766 reads in 245.523 CPU sec, 7.927 real sec
sorting block 37, #reads = 1079605 mem = 536880618 peak = 1197264896
[M::process] read 672688 sequences (100000043 bp) in 3.028 CPU sec, 3.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284624, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.25, 146.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672644 sequences (100000260 bp) in 1.997 CPU sec, 2.066 real sec...
[M::process] read 673136 sequences (100000053 bp) in 2.030 CPU sec, 2.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284371, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.53, 147.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 168, 600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1480)
[M::mem_pestat] mean and std.dev: (323.20, 279.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1920)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672806 reads in 238.990 CPU sec, 7.549 real sec
sorting block 38, #reads = 1078887 mem = 536881110 peak = 1197264896
[M::mem_process_seqs] Processed 672688 reads in 133.262 CPU sec, 4.198 real sec
sorting block 39, #reads = 1078989 mem = 536878140 peak = 1202569216
[M::process] read 674018 sequences (100000095 bp) in 2.604 CPU sec, 2.664 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284716, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.66, 149.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (90, 501, 1132)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3216)
[M::mem_pestat] mean and std.dev: (356.88, 390.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4258)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673992 sequences (100000293 bp) in 2.030 CPU sec, 2.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286212, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.24, 123.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285455, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.92, 142.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672644 reads in 325.569 CPU sec, 10.386 real sec
[M::mem_process_seqs] Processed 674018 reads in 223.173 CPU sec, 7.145 real sec
[M::mem_process_seqs] Processed 673136 reads in 232.950 CPU sec, 7.524 real sec
sorting block 40, #reads = 1079104 mem = 536880105 peak = 1202569216
sorting block 41, #reads = 1080039 mem = 536877581 peak = 1202569216
[M::process] read 673824 sequences (100000101 bp) in 3.058 CPU sec, 3.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286386, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.11, 124.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673664 sequences (100000278 bp) in 2.090 CPU sec, 2.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286218, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (254.91, 126.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 148, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175)
[M::mem_pestat] mean and std.dev: (299.27, 392.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1869)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673992 reads in 224.061 CPU sec, 7.084 real sec
[M::process] read 673520 sequences (100000269 bp) in 2.040 CPU sec, 2.032 real sec...
[M::mem_process_seqs] Processed 673824 reads in 125.454 CPU sec, 3.927 real sec
sorting block 42, #reads = 1080387 mem = 536882183 peak = 1202569216
[M::process] read 673410 sequences (100000098 bp) in 2.326 CPU sec, 2.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285802, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.22, 129.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 33, 187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (43.12, 60.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 724)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673320 sequences (100000172 bp) in 1.500 CPU sec, 1.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285392, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.53, 133.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 160, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1802)
[M::mem_pestat] mean and std.dev: (243.30, 249.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2399)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286072, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.95, 131.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673664 reads in 324.833 CPU sec, 10.390 real sec
[M::mem_process_seqs] Processed 673410 reads in 225.364 CPU sec, 7.248 real sec
[M::mem_process_seqs] Processed 673520 reads in 234.825 CPU sec, 7.566 real sec
sorting block 43, #reads = 1080331 mem = 536882420 peak = 1202569216
sorting block 44, #reads = 1080449 mem = 536879340 peak = 1202569216
[M::process] read 673408 sequences (100000263 bp) in 2.908 CPU sec, 3.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285160, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (263.51, 133.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673090 sequences (100000221 bp) in 2.107 CPU sec, 2.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285444, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.11, 135.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673148 sequences (100000099 bp) in 2.020 CPU sec, 2.017 real sec...
[M::mem_process_seqs] Processed 673320 reads in 233.852 CPU sec, 7.406 real sec
[M::mem_process_seqs] Processed 673408 reads in 132.076 CPU sec, 4.149 real sec
sorting block 45, #reads = 1080072 mem = 536880440 peak = 1202569216
[M::process] read 673114 sequences (100000288 bp) in 2.642 CPU sec, 2.703 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284984, 25, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.98, 139.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (56, 263, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1853)
[M::mem_pestat] mean and std.dev: (368.21, 373.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2452)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673062 sequences (100000116 bp) in 2.000 CPU sec, 2.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285097, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.62, 142.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284712, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.71, 142.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673090 reads in 340.409 CPU sec, 10.864 real sec
[M::mem_process_seqs] Processed 673148 reads in 235.848 CPU sec, 7.558 real sec
sorting block 46, #reads = 1080278 mem = 536878823 peak = 1202569216
[M::mem_process_seqs] Processed 673114 reads in 246.001 CPU sec, 8.066 real sec
sorting block 47, #reads = 1079462 mem = 536880592 peak = 1202569216
[M::process] read 673188 sequences (100000244 bp) in 2.938 CPU sec, 3.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284299, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.58, 144.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673090 sequences (100000071 bp) in 2.126 CPU sec, 2.128 real sec...
[M::process] read 673006 sequences (100000095 bp) in 2.020 CPU sec, 2.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284777, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.83, 147.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 199, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (206.33, 185.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673062 reads in 240.673 CPU sec, 7.608 real sec
[M::mem_process_seqs] Processed 673188 reads in 132.506 CPU sec, 4.168 real sec
sorting block 48, #reads = 1079189 mem = 536878516 peak = 1202569216
[M::process] read 673744 sequences (100000220 bp) in 2.360 CPU sec, 2.417 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284439, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.35, 147.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 55, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235)
[M::mem_pestat] mean and std.dev: (43.09, 35.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 309)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674182 sequences (100000226 bp) in 2.050 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284393, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.78, 147.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286116, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (257.87, 132.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 130, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (121.18, 116.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673090 reads in 337.110 CPU sec, 10.772 real sec
[M::mem_process_seqs] Processed 673006 reads in 228.708 CPU sec, 7.347 real sec
sorting block 49, #reads = 1079812 mem = 536878364 peak = 1202569216
[M::mem_process_seqs] Processed 673744 reads in 238.575 CPU sec, 7.680 real sec
sorting block 50, #reads = 1079642 mem = 536880078 peak = 1202569216
[M::process] read 674120 sequences (100000235 bp) in 3.001 CPU sec, 3.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286410, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.56, 124.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673926 sequences (100000076 bp) in 2.130 CPU sec, 2.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286467, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.63, 127.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674182 reads in 215.306 CPU sec, 6.793 real sec
[M::mem_process_seqs] Processed 674120 reads in 120.011 CPU sec, 3.756 real sec
[M::process] read 673682 sequences (100000136 bp) in 2.277 CPU sec, 2.279 real sec...
sorting block 51, #reads = 1080129 mem = 536877245 peak = 1202569216
[M::process] read 673640 sequences (100000130 bp) in 2.638 CPU sec, 2.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285973, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (255.48, 130.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 47, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181)
[M::mem_pestat] mean and std.dev: (40.73, 28.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 235)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673544 sequences (100000112 bp) in 2.020 CPU sec, 2.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286118, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (258.79, 132.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285828, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (260.99, 134.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 114, 1034)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3020)
[M::mem_pestat] mean and std.dev: (375.64, 444.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4013)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673926 reads in 317.507 CPU sec, 10.121 real sec
[M::mem_process_seqs] Processed 673682 reads in 220.947 CPU sec, 7.066 real sec
sorting block 52, #reads = 1080278 mem = 536877630 peak = 1202569216
[M::mem_process_seqs] Processed 673640 reads in 230.481 CPU sec, 7.387 real sec
sorting block 53, #reads = 1080218 mem = 536877838 peak = 1242349568
[M::process] read 673566 sequences (100000250 bp) in 3.080 CPU sec, 3.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285555, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.64, 136.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673418 sequences (100000156 bp) in 2.159 CPU sec, 2.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285441, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.58, 135.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673544 reads in 228.218 CPU sec, 7.211 real sec
[M::process] read 673452 sequences (100000057 bp) in 2.079 CPU sec, 2.084 real sec...
[M::mem_process_seqs] Processed 673566 reads in 126.688 CPU sec, 3.968 real sec
sorting block 54, #reads = 1080010 mem = 536879886 peak = 1242349568
[M::process] read 673258 sequences (100000269 bp) in 2.265 CPU sec, 2.308 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285142, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (265.30, 138.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 155, 664)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1922)
[M::mem_pestat] mean and std.dev: (420.36, 436.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2551)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673180 sequences (100000032 bp) in 1.530 CPU sec, 1.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284904, 21, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (268.51, 141.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 73, 169)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491)
[M::mem_pestat] mean and std.dev: (61.32, 58.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 652)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285364, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (267.49, 139.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673418 reads in 335.882 CPU sec, 10.745 real sec
sorting block 55, #reads = 1080032 mem = 536879384 peak = 1242349568
[M::mem_process_seqs] Processed 673258 reads in 232.742 CPU sec, 7.488 real sec
[M::mem_process_seqs] Processed 673452 reads in 242.979 CPU sec, 7.832 real sec
sorting block 56, #reads = 1079709 mem = 536880717 peak = 1246052352
[M::process] read 673098 sequences (100000071 bp) in 3.141 CPU sec, 3.266 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284921, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.42, 143.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673118 sequences (100000152 bp) in 2.100 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284927, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.12, 145.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 98, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226)
[M::mem_pestat] mean and std.dev: (212.90, 230.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1628)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672870 sequences (100000175 bp) in 2.040 CPU sec, 2.039 real sec...
[M::mem_process_seqs] Processed 673180 reads in 238.800 CPU sec, 7.556 real sec
sorting block 57, #reads = 1079641 mem = 536878742 peak = 1246052352
[M::mem_process_seqs] Processed 673098 reads in 132.885 CPU sec, 4.187 real sec
sorting block 58, #reads = 1079203 mem = 536880210 peak = 1246052352
[M::process] read 673188 sequences (100000269 bp) in 2.698 CPU sec, 2.766 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284402, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (273.92, 145.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 71, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222)
[M::mem_pestat] mean and std.dev: (50.27, 33.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 290)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674408 sequences (100000017 bp) in 2.000 CPU sec, 2.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285653, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (267.55, 141.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284534, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (274.93, 147.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673118 reads in 335.684 CPU sec, 10.708 real sec
[M::mem_process_seqs] Processed 673188 reads in 229.278 CPU sec, 7.354 real sec
[M::mem_process_seqs] Processed 672870 reads in 239.180 CPU sec, 7.853 real sec
sorting block 59, #reads = 1079495 mem = 536879568 peak = 1246052352
[M::process] read 674204 sequences (100000026 bp) in 3.132 CPU sec, 3.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286602, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (246.59, 123.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673984 sequences (100000176 bp) in 2.070 CPU sec, 2.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285882, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.43, 125.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (57, 674, 867)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2487)
[M::mem_pestat] mean and std.dev: (498.50, 490.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3297)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674408 reads in 223.251 CPU sec, 7.065 real sec
sorting block 60, #reads = 1079587 mem = 536878471 peak = 1246052352
[M::mem_process_seqs] Processed 674204 reads in 125.238 CPU sec, 3.916 real sec
[M::process] read 673874 sequences (100000136 bp) in 2.129 CPU sec, 2.131 real sec...
sorting block 61, #reads = 1080555 mem = 536877878 peak = 1253904384
[M::process] read 673746 sequences (100000136 bp) in 2.662 CPU sec, 2.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285318, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.94, 128.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 78, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1937)
[M::mem_pestat] mean and std.dev: (149.12, 221.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2575)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673618 sequences (100000065 bp) in 2.020 CPU sec, 2.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285518, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.17, 131.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 219, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (112.09, 92.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285480, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (255.91, 130.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (65, 184, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (125.38, 77.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 701)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673984 reads in 330.821 CPU sec, 10.554 real sec
[M::mem_process_seqs] Processed 673746 reads in 226.524 CPU sec, 7.256 real sec
[M::mem_process_seqs] Processed 673874 reads in 235.978 CPU sec, 7.573 real sec
sorting block 62, #reads = 1079904 mem = 536880931 peak = 1253904384
[M::process] read 673470 sequences (100000141 bp) in 2.405 CPU sec, 2.533 real sec...
sorting block 63, #reads = 1080442 mem = 536880697 peak = 1253904384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285179, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.74, 135.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673482 sequences (100000238 bp) in 1.771 CPU sec, 1.774 real sec...
[M::process] read 673460 sequences (100000164 bp) in 1.550 CPU sec, 1.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285262, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.97, 135.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673618 reads in 234.431 CPU sec, 7.467 real sec
[M::mem_process_seqs] Processed 673470 reads in 129.634 CPU sec, 4.111 real sec
sorting block 64, #reads = 1080157 mem = 536877839 peak = 1253904384
[M::process] read 673344 sequences (100000130 bp) in 2.648 CPU sec, 2.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284970, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.70, 139.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673220 sequences (100000201 bp) in 2.000 CPU sec, 1.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284800, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.55, 139.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284737, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (266.90, 140.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673482 reads in 345.118 CPU sec, 11.014 real sec
[M::mem_process_seqs] Processed 673344 reads in 238.076 CPU sec, 7.638 real sec
[M::mem_process_seqs] Processed 673460 reads in 247.992 CPU sec, 8.117 real sec
sorting block 65, #reads = 1079842 mem = 536878787 peak = 1253904384
[M::process] read 673208 sequences (100000226 bp) in 2.787 CPU sec, 2.898 real sec...
sorting block 66, #reads = 1079994 mem = 536880683 peak = 1253904384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284636, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (268.87, 141.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673226 sequences (100000027 bp) in 1.713 CPU sec, 1.719 real sec...
[M::process] read 673080 sequences (100000269 bp) in 1.490 CPU sec, 1.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284577, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.07, 144.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 98, 4335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12965)
[M::mem_pestat] mean and std.dev: (1485.40, 1926.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17280)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673220 reads in 242.628 CPU sec, 7.725 real sec
[M::mem_process_seqs] Processed 673208 reads in 133.556 CPU sec, 4.237 real sec
sorting block 67, #reads = 1079459 mem = 536878086 peak = 1253904384
[M::process] read 673218 sequences (100000010 bp) in 2.886 CPU sec, 2.942 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284859, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.18, 145.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674140 sequences (100000222 bp) in 2.040 CPU sec, 2.047 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285059, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (272.45, 146.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (53, 342, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1937)
[M::mem_pestat] mean and std.dev: (321.00, 283.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2565)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284641, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.19, 147.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 157, 668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1984)
[M::mem_pestat] mean and std.dev: (472.67, 636.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3020)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673226 reads in 340.863 CPU sec, 10.869 real sec
[M::mem_process_seqs] Processed 673218 reads in 235.456 CPU sec, 7.544 real sec
[M::mem_process_seqs] Processed 673080 reads in 245.165 CPU sec, 7.876 real sec
sorting block 68, #reads = 1079431 mem = 536878372 peak = 1253904384
sorting block 69, #reads = 1079609 mem = 536879738 peak = 1253904384
[M::process] read 674158 sequences (100000123 bp) in 3.161 CPU sec, 3.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286196, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.75, 124.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 65, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086)
[M::mem_pestat] mean and std.dev: (139.07, 152.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1444)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674110 sequences (100000116 bp) in 2.060 CPU sec, 2.059 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286382, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.60, 125.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674140 reads in 226.479 CPU sec, 7.166 real sec
[M::mem_process_seqs] Processed 674158 reads in 126.519 CPU sec, 3.957 real sec
[M::process] read 673980 sequences (100000183 bp) in 2.108 CPU sec, 2.108 real sec...
sorting block 70, #reads = 1080028 mem = 536878462 peak = 1253904384
[M::process] read 673918 sequences (100000173 bp) in 2.710 CPU sec, 2.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286323, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.79, 127.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 132, 158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (80.89, 72.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 602)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673754 sequences (100000109 bp) in 2.010 CPU sec, 2.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285452, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (254.90, 129.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 25, 43)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 79)
[M::mem_pestat] mean and std.dev: (29.80, 14.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 97)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285041, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.32, 130.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 73, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152)
[M::mem_pestat] mean and std.dev: (179.42, 206.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1528)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674110 reads in 330.787 CPU sec, 10.554 real sec
[M::mem_process_seqs] Processed 673980 reads in 231.157 CPU sec, 7.398 real sec
[M::mem_process_seqs] Processed 673918 reads in 240.919 CPU sec, 7.730 real sec
sorting block 71, #reads = 1080305 mem = 536877615 peak = 1253904384
sorting block 72, #reads = 1080450 mem = 536879018 peak = 1269854208
[M::process] read 673538 sequences (100000153 bp) in 2.892 CPU sec, 3.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285076, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.65, 133.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673356 sequences (100000106 bp) in 2.047 CPU sec, 2.083 real sec...
[M::process] read 673468 sequences (100000293 bp) in 2.040 CPU sec, 2.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284625, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.69, 136.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673754 reads in 242.220 CPU sec, 7.656 real sec
[M::mem_process_seqs] Processed 673538 reads in 132.747 CPU sec, 4.177 real sec
sorting block 73, #reads = 1080161 mem = 536877399 peak = 1269854208
[M::process] read 673442 sequences (100000148 bp) in 2.637 CPU sec, 2.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284533, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.44, 139.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673256 sequences (100000063 bp) in 1.980 CPU sec, 1.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284904, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.10, 138.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284627, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.71, 140.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 86, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2138)
[M::mem_pestat] mean and std.dev: (297.25, 451.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2841)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673356 reads in 349.394 CPU sec, 11.157 real sec
[M::mem_process_seqs] Processed 673468 reads in 241.071 CPU sec, 7.735 real sec
sorting block 74, #reads = 1080132 mem = 536877804 peak = 1269854208
[M::mem_process_seqs] Processed 673442 reads in 250.694 CPU sec, 8.061 real sec
sorting block 75, #reads = 1079651 mem = 536880049 peak = 1286160384
[M::process] read 673134 sequences (100000022 bp) in 2.626 CPU sec, 2.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283695, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.37, 143.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673104 sequences (100000093 bp) in 2.094 CPU sec, 2.150 real sec...
[M::process] read 673106 sequences (100000127 bp) in 2.040 CPU sec, 2.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284183, 15, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.03, 144.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (104, 519, 1138)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3206)
[M::mem_pestat] mean and std.dev: (546.00, 459.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4240)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673256 reads in 253.130 CPU sec, 8.030 real sec
[M::mem_process_seqs] Processed 673134 reads in 136.436 CPU sec, 4.305 real sec
sorting block 76, #reads = 1079935 mem = 536881093 peak = 1286160384
[M::process] read 672922 sequences (100000011 bp) in 2.274 CPU sec, 2.348 real sec...
[M::process] read 327222 sequences (48625733 bp) in 0.800 CPU sec, 0.794 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283848, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (275.46, 147.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283988, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (276.33, 148.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283977, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (279.43, 151.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673104 reads in 354.226 CPU sec, 11.360 real sec
sorting block 77, #reads = 1079534 mem = 536878434 peak = 1295802368
[M::mem_process_seqs] Processed 673106 reads in 242.642 CPU sec, 7.829 real sec
[M::mem_process_seqs] Processed 672922 reads in 252.831 CPU sec, 8.173 real sec
sorting block 78, #reads = 1079604 mem = 536877991 peak = 1305460736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 138004, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (278.08, 150.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 327222 reads in 63.626 CPU sec, 2.133 real sec
sorting block 79, #reads = 1079260 mem = 536884554 peak = 1305460736
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: 1f8bfbff fffa320b
  extended: d19f47ab 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 85844588
overall: 70018199552 mem 16538.425 user 154.098 sys 1025.722 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L008\tPU:L008\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.T18-D0003255_R1.fastq.gz 0004.T18-D0003255_R2.fastq.gz
[main] Real time: 1025.758 sec; CPU: 16692.532 sec
sorting block 80, #reads = 85629 mem = 42609192 peak = 1305460736
uniq header count 1
merging 81 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: 1f8bfbff fffa320b
  extended: d19f47ab 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31 max 48
algo: util-sort
license: sentieon:util=1
output file size: 3304791606
output reads: 86472551
bam_mem_sort: 81 calls 79.091 user 0.097 sys 79.340 real
bam_write: 81 calls 22.634 user 1.517 sys 24.551 real
execute: 1 calls 26.521 user 0.800 sys 1054.976 real
merge_files: 1 calls 26.491 user 0.769 sys 29.184 real
parse_chunk: 1282 calls 113.866 user 20.912 sys 136.010 real
read_chunk: 3450 calls 43.591 user 27.500 sys 503.496 real
sort_block: 1 calls 31.082 user 26.490 sys 485.874 real
write_chunk: 2166 calls 25.832 user 0.559 sys 27.804 real
overall: 2724904960 mem 1250.706 user 61.099 sys 1054.996 real
ls: cannot access '*.cram': No such file or directory