This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 2100.995 CPU sec, 501.383 real sec
[M::process] read 673452 sequences (100000007 bp) in 1.159 CPU sec, 1.163 real sec...
[M::process] read 673040 sequences (100000221 bp) in 1.740 CPU sec, 1.745 real sec...
[M::process] read 673010 sequences (100000088 bp) in 1.740 CPU sec, 1.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286144, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.77, 132.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673012 sequences (100000221 bp) in 1.548 CPU sec, 1.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285440, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.68, 138.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 43, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (88.27, 111.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286005, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (269.44, 136.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673452 reads in 306.066 CPU sec, 9.775 real sec
[M::mem_process_seqs] Processed 673010 reads in 193.978 CPU sec, 6.264 real sec
[M::mem_process_seqs] Processed 673040 reads in 202.060 CPU sec, 6.553 real sec
sorting block 0, #reads = 1079697 mem = 536878733 peak = 604033024
[M::process] read 672766 sequences (100000044 bp) in 2.315 CPU sec, 2.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285570, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (272.88, 139.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672734 sequences (100000024 bp) in 1.577 CPU sec, 1.627 real sec...
[M::process] read 672572 sequences (100000036 bp) in 1.448 CPU sec, 1.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285548, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.19, 143.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673012 reads in 196.639 CPU sec, 6.271 real sec
sorting block 1, #reads = 1079660 mem = 536879141 peak = 758173696
[M::mem_process_seqs] Processed 672766 reads in 109.440 CPU sec, 3.471 real sec
sorting block 2, #reads = 1079718 mem = 536880535 peak = 866217984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285391, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (283.44, 147.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672540 sequences (100000227 bp) in 2.599 CPU sec, 2.920 real sec...
[M::process] read 672758 sequences (100000276 bp) in 1.450 CPU sec, 1.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284833, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (286.76, 150.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672734 reads in 200.968 CPU sec, 6.415 real sec
[M::mem_process_seqs] Processed 672572 reads in 111.503 CPU sec, 3.570 real sec
sorting block 3, #reads = 1079904 mem = 536879591 peak = 866217984
[M::process] read 672796 sequences (100000028 bp) in 2.143 CPU sec, 2.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285167, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (287.88, 152.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673198 sequences (100000241 bp) in 1.430 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284831, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.92, 148.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285343, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.23, 146.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672540 reads in 291.606 CPU sec, 9.366 real sec
[M::mem_process_seqs] Processed 672758 reads in 202.844 CPU sec, 6.550 real sec
[M::mem_process_seqs] Processed 672796 reads in 210.719 CPU sec, 6.826 real sec
sorting block 4, #reads = 1079610 mem = 536878653 peak = 866217984
[M::process] read 673128 sequences (100000168 bp) in 2.832 CPU sec, 3.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285690, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.34, 136.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 5, #reads = 1079440 mem = 536880986 peak = 866217984
[M::process] read 673098 sequences (100000025 bp) in 2.071 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285643, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.62, 138.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 380, 675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1999)
[M::mem_pestat] mean and std.dev: (380.83, 397.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2661)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673198 reads in 209.390 CPU sec, 6.706 real sec
[M::mem_process_seqs] Processed 673128 reads in 117.403 CPU sec, 3.735 real sec
[M::process] read 672930 sequences (100000258 bp) in 1.873 CPU sec, 2.003 real sec...
sorting block 6, #reads = 1080149 mem = 536877844 peak = 866217984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285698, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.58, 140.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672936 sequences (100000027 bp) in 2.675 CPU sec, 3.040 real sec...
[M::process] read 672798 sequences (100000174 bp) in 1.520 CPU sec, 1.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285450, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.85, 142.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (241, 264, 664)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1510)
[M::mem_pestat] mean and std.dev: (291.10, 222.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1933)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673098 reads in 205.941 CPU sec, 6.596 real sec
[M::mem_process_seqs] Processed 672930 reads in 116.762 CPU sec, 3.736 real sec
sorting block 7, #reads = 1079840 mem = 536878181 peak = 866217984
[M::process] read 672936 sequences (100000297 bp) in 2.035 CPU sec, 2.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285199, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (275.80, 143.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 65, 1087)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3203)
[M::mem_pestat] mean and std.dev: (518.75, 718.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4261)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673198 sequences (100000090 bp) in 1.410 CPU sec, 1.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284932, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.92, 145.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (72, 233, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (216.55, 173.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1356)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284701, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.88, 143.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672936 reads in 296.039 CPU sec, 9.527 real sec
[M::mem_process_seqs] Processed 672798 reads in 204.369 CPU sec, 6.603 real sec
sorting block 8, #reads = 1080018 mem = 536881991 peak = 866217984
[M::mem_process_seqs] Processed 672936 reads in 212.661 CPU sec, 6.896 real sec
sorting block 9, #reads = 1079333 mem = 536880175 peak = 866217984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286206, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (266.73, 133.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 65, 731)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2143)
[M::mem_pestat] mean and std.dev: (396.55, 584.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2849)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673198 reads in 103.151 CPU sec, 3.417 real sec
[M::process] read 673052 sequences (100000136 bp) in 3.252 CPU sec, 3.744 real sec...
sorting block 10, #reads = 1079247 mem = 536878152 peak = 866217984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285760, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (271.87, 138.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673052 reads in 96.496 CPU sec, 3.155 real sec
[M::process] read 673036 sequences (100000238 bp) in 2.788 CPU sec, 3.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285442, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.95, 139.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673014 sequences (100000227 bp) in 2.528 CPU sec, 3.000 real sec...
[M::mem_process_seqs] Processed 673036 reads in 94.612 CPU sec, 3.058 real sec
sorting block 11, #reads = 1079978 mem = 536879748 peak = 866217984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285091, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (276.20, 142.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 33, 180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520)
[M::mem_pestat] mean and std.dev: (44.91, 50.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672908 sequences (100000039 bp) in 2.729 CPU sec, 3.050 real sec...
[M::mem_process_seqs] Processed 673014 reads in 95.281 CPU sec, 3.085 real sec
sorting block 12, #reads = 1079497 mem = 536880200 peak = 866217984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284851, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.03, 145.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672908 reads in 105.639 CPU sec, 3.426 real sec
[M::process] read 672582 sequences (100000029 bp) in 3.523 CPU sec, 4.083 real sec...
[M::process] read 672480 sequences (100000170 bp) in 2.385 CPU sec, 2.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284841, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (286.10, 149.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672470 sequences (100000221 bp) in 1.270 CPU sec, 1.266 real sec...
[M::process] read 672774 sequences (100000041 bp) in 1.170 CPU sec, 1.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283939, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 211, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (288.87, 151.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672582 reads in 207.383 CPU sec, 6.599 real sec
[M::mem_process_seqs] Processed 672480 reads in 116.679 CPU sec, 3.743 real sec
sorting block 13, #reads = 1080066 mem = 536879448 peak = 866217984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284415, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (287.70, 152.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1079513 mem = 536878948 peak = 866217984
[M::process] read 672912 sequences (100000058 bp) in 3.067 CPU sec, 3.529 real sec...
[M::process] read 673392 sequences (100000044 bp) in 1.549 CPU sec, 1.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284180, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (283.66, 149.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 62, 188)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (62.50, 63.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 692)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672470 reads in 205.707 CPU sec, 6.590 real sec
[M::mem_process_seqs] Processed 672774 reads in 113.469 CPU sec, 3.617 real sec
sorting block 15, #reads = 1079068 mem = 536880706 peak = 866217984
[M::process] read 673116 sequences (100000247 bp) in 2.405 CPU sec, 2.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284570, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.62, 143.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673006 sequences (100000152 bp) in 1.210 CPU sec, 1.212 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286004, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.82, 133.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 86, 113)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291)
[M::mem_pestat] mean and std.dev: (73.09, 47.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 380)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284967, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.28, 141.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 180, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2164)
[M::mem_pestat] mean and std.dev: (300.50, 392.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2880)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672912 reads in 276.574 CPU sec, 8.882 real sec
[M::mem_process_seqs] Processed 673392 reads in 185.151 CPU sec, 5.988 real sec
[M::mem_process_seqs] Processed 673116 reads in 192.927 CPU sec, 6.264 real sec
sorting block 16, #reads = 1079320 mem = 536877229 peak = 866217984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284887, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.96, 143.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1079763 mem = 536877838 peak = 866217984
[M::mem_process_seqs] Processed 673006 reads in 99.483 CPU sec, 3.279 real sec
[M::process] read 672950 sequences (100000241 bp) in 3.412 CPU sec, 3.968 real sec...
[M::process] read 672898 sequences (100000201 bp) in 1.591 CPU sec, 1.726 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284540, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.46, 144.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 41, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1234)
[M::mem_pestat] mean and std.dev: (125.85, 164.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1641)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672726 sequences (100000250 bp) in 1.140 CPU sec, 1.142 real sec...
[M::process] read 672990 sequences (100000182 bp) in 1.120 CPU sec, 1.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285058, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.59, 146.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 69, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (81.22, 71.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 727)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672950 reads in 177.104 CPU sec, 5.625 real sec
[M::mem_process_seqs] Processed 672898 reads in 98.674 CPU sec, 3.172 real sec
sorting block 18, #reads = 1079828 mem = 536877580 peak = 866217984
sorting block 19, #reads = 1079284 mem = 536877321 peak = 929705984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284286, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (283.14, 149.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673132 sequences (100000106 bp) in 2.498 CPU sec, 2.734 real sec...
[M::process] read 673014 sequences (100000216 bp) in 1.390 CPU sec, 1.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285629, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.30, 139.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672726 reads in 174.036 CPU sec, 5.560 real sec
[M::mem_process_seqs] Processed 672990 reads in 95.927 CPU sec, 3.069 real sec
sorting block 20, #reads = 1078422 mem = 536877882 peak = 929705984
[M::process] read 673252 sequences (100000227 bp) in 1.773 CPU sec, 1.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285653, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (268.45, 135.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673164 sequences (100000260 bp) in 1.430 CPU sec, 1.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285837, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.97, 134.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285506, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (269.14, 135.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673132 reads in 250.428 CPU sec, 8.036 real sec
[M::mem_process_seqs] Processed 673252 reads in 175.831 CPU sec, 5.662 real sec
[M::mem_process_seqs] Processed 673014 reads in 183.216 CPU sec, 5.925 real sec
sorting block 21, #reads = 1080092 mem = 536877672 peak = 929705984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285931, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.29, 136.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 65, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1622)
[M::mem_pestat] mean and std.dev: (102.62, 172.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2159)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673002 sequences (100000140 bp) in 2.541 CPU sec, 2.862 real sec...
[M::mem_process_seqs] Processed 673164 reads in 92.068 CPU sec, 3.054 real sec
sorting block 22, #reads = 1080389 mem = 536877038 peak = 929705984
[M::process] read 672788 sequences (100000276 bp) in 2.220 CPU sec, 2.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285090, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.70, 141.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (83, 91, 1814)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5276)
[M::mem_pestat] mean and std.dev: (788.85, 833.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7007)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672716 sequences (100000138 bp) in 1.460 CPU sec, 1.458 real sec...
[M::process] read 672626 sequences (100000053 bp) in 1.140 CPU sec, 1.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285112, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.14, 145.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 225, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (135.80, 133.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673002 reads in 177.220 CPU sec, 5.630 real sec
[M::mem_process_seqs] Processed 672788 reads in 99.477 CPU sec, 3.170 real sec
sorting block 23, #reads = 1079836 mem = 536883386 peak = 929705984
sorting block 24, #reads = 1079759 mem = 536877976 peak = 946229248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284722, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 205, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (282.15, 146.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672710 sequences (100000126 bp) in 2.720 CPU sec, 2.988 real sec...
[M::process] read 673304 sequences (100000013 bp) in 1.230 CPU sec, 1.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284875, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (282.93, 147.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 85, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (109.27, 100.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672716 reads in 180.619 CPU sec, 5.762 real sec
[M::mem_process_seqs] Processed 672626 reads in 99.422 CPU sec, 3.182 real sec
sorting block 25, #reads = 1079357 mem = 536877657 peak = 946229248
[M::process] read 673250 sequences (100000024 bp) in 1.829 CPU sec, 2.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284959, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.46, 148.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 33, 37)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 89)
[M::mem_pestat] mean and std.dev: (28.55, 17.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 115)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673030 sequences (100000081 bp) in 1.270 CPU sec, 1.264 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285018, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.40, 136.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 86, 98)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218)
[M::mem_pestat] mean and std.dev: (62.17, 27.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 278)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285368, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.42, 136.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 239, 866)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2426)
[M::mem_pestat] mean and std.dev: (449.62, 543.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3206)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672710 reads in 258.162 CPU sec, 8.285 real sec
[M::mem_process_seqs] Processed 673304 reads in 178.025 CPU sec, 5.736 real sec
[M::mem_process_seqs] Processed 673250 reads in 185.251 CPU sec, 5.992 real sec
sorting block 26, #reads = 1079411 mem = 536879462 peak = 946229248
[M::process] read 673004 sequences (100000143 bp) in 2.513 CPU sec, 2.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284494, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.22, 141.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 292, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (268.06, 193.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 27, #reads = 1079947 mem = 536879831 peak = 946229248
[M::process] read 672786 sequences (100000298 bp) in 1.962 CPU sec, 2.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284584, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.15, 143.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (81, 204, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (149.45, 102.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 709)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673030 reads in 186.268 CPU sec, 5.964 real sec
[M::process] read 672806 sequences (100000130 bp) in 1.161 CPU sec, 1.158 real sec...
[M::mem_process_seqs] Processed 673004 reads in 103.274 CPU sec, 3.278 real sec
sorting block 28, #reads = 1079546 mem = 536878012 peak = 946229248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284651, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.87, 147.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672704 sequences (100000122 bp) in 2.354 CPU sec, 2.688 real sec...
[M::process] read 673328 sequences (100000039 bp) in 1.300 CPU sec, 1.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284698, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.59, 146.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672786 reads in 179.951 CPU sec, 5.745 real sec
[M::mem_process_seqs] Processed 672806 reads in 99.111 CPU sec, 3.179 real sec
sorting block 29, #reads = 1079630 mem = 536879464 peak = 946229248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284681, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.94, 148.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673466 sequences (100000272 bp) in 1.934 CPU sec, 2.128 real sec...
[M::process] read 673500 sequences (100000242 bp) in 1.500 CPU sec, 1.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285418, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.69, 133.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672704 reads in 175.104 CPU sec, 5.606 real sec
[M::mem_process_seqs] Processed 673328 reads in 96.310 CPU sec, 3.084 real sec
sorting block 30, #reads = 1079512 mem = 536879147 peak = 946229248
sorting block 31, #reads = 1079134 mem = 536877326 peak = 956571648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285615, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.26, 132.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673406 sequences (100000030 bp) in 2.510 CPU sec, 2.738 real sec...
[M::process] read 673198 sequences (100000239 bp) in 1.470 CPU sec, 1.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285274, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (258.76, 127.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673466 reads in 173.250 CPU sec, 5.523 real sec
[M::mem_process_seqs] Processed 673500 reads in 96.765 CPU sec, 3.088 real sec
sorting block 32, #reads = 1079955 mem = 536878823 peak = 956571648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286165, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (260.38, 128.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 259, 653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1705)
[M::mem_pestat] mean and std.dev: (336.81, 282.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2231)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673042 sequences (100000084 bp) in 2.364 CPU sec, 2.553 real sec...
[M::process] read 673000 sequences (100000127 bp) in 1.400 CPU sec, 1.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285222, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (266.44, 134.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 754)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2256)
[M::mem_pestat] mean and std.dev: (262.33, 370.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3007)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673406 reads in 173.990 CPU sec, 5.557 real sec
[M::mem_process_seqs] Processed 673198 reads in 97.613 CPU sec, 3.116 real sec
sorting block 33, #reads = 1079946 mem = 536879851 peak = 956571648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284701, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.71, 138.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672888 sequences (100000293 bp) in 2.513 CPU sec, 2.700 real sec...
[M::process] read 672928 sequences (100000228 bp) in 1.390 CPU sec, 1.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284615, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.59, 140.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673042 reads in 179.054 CPU sec, 5.710 real sec
[M::mem_process_seqs] Processed 673000 reads in 99.098 CPU sec, 3.176 real sec
sorting block 34, #reads = 1079696 mem = 536878523 peak = 956571648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284925, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (274.27, 140.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 45, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233)
[M::mem_pestat] mean and std.dev: (45.67, 39.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 306)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673020 sequences (100000188 bp) in 2.052 CPU sec, 2.272 real sec...
[M::process] read 673234 sequences (100000172 bp) in 1.290 CPU sec, 1.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284424, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (271.76, 138.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 231, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296)
[M::mem_pestat] mean and std.dev: (272.50, 229.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1716)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672888 reads in 177.454 CPU sec, 5.679 real sec
[M::mem_process_seqs] Processed 672928 reads in 98.699 CPU sec, 3.166 real sec
sorting block 35, #reads = 1080033 mem = 536880157 peak = 956571648
sorting block 36, #reads = 1079298 mem = 536879372 peak = 956571648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284995, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.81, 137.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673082 sequences (100000159 bp) in 2.360 CPU sec, 2.613 real sec...
[M::process] read 673056 sequences (100000149 bp) in 1.280 CPU sec, 1.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285729, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.29, 132.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 400, 1598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4728)
[M::mem_pestat] mean and std.dev: (550.13, 650.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6293)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673020 reads in 174.439 CPU sec, 5.571 real sec
[M::mem_process_seqs] Processed 673234 reads in 96.857 CPU sec, 3.103 real sec
sorting block 37, #reads = 1079831 mem = 536879013 peak = 956571648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285146, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.85, 136.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672946 sequences (100000103 bp) in 2.208 CPU sec, 2.433 real sec...
[M::process] read 672788 sequences (100000262 bp) in 1.360 CPU sec, 1.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284711, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.74, 139.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (379, 1208, 9375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 27367)
[M::mem_pestat] mean and std.dev: (3072.73, 3891.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 36363)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673082 reads in 175.770 CPU sec, 5.619 real sec
[M::mem_process_seqs] Processed 673056 reads in 98.597 CPU sec, 3.153 real sec
sorting block 38, #reads = 1080300 mem = 536877718 peak = 956571648
[M::process] read 672712 sequences (100000255 bp) in 2.017 CPU sec, 2.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284310, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.99, 141.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672578 sequences (100000287 bp) in 1.190 CPU sec, 1.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284523, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.20, 145.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284788, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.54, 148.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672946 reads in 259.529 CPU sec, 8.326 real sec
[M::mem_process_seqs] Processed 672788 reads in 180.677 CPU sec, 5.826 real sec
[M::mem_process_seqs] Processed 672712 reads in 188.180 CPU sec, 6.092 real sec
sorting block 39, #reads = 1079579 mem = 536880910 peak = 956571648
sorting block 40, #reads = 1079632 mem = 536882525 peak = 956571648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284806, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.68, 146.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673366 sequences (100000277 bp) in 2.489 CPU sec, 2.907 real sec...
[M::mem_process_seqs] Processed 672578 reads in 94.154 CPU sec, 3.099 real sec
sorting block 41, #reads = 1078908 mem = 536879495 peak = 956571648
[M::process] read 673164 sequences (100000191 bp) in 2.451 CPU sec, 2.895 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285346, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.75, 139.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 653, 653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1957)
[M::mem_pestat] mean and std.dev: (291.00, 323.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2609)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673170 sequences (100000237 bp) in 1.085 CPU sec, 1.190 real sec...
[M::process] read 673092 sequences (100000131 bp) in 1.090 CPU sec, 1.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285114, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.43, 141.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673366 reads in 176.683 CPU sec, 5.644 real sec
[M::mem_process_seqs] Processed 673164 reads in 97.722 CPU sec, 3.118 real sec
sorting block 42, #reads = 1079764 mem = 536879698 peak = 956571648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284994, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.73, 141.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673006 sequences (100000247 bp) in 2.561 CPU sec, 2.919 real sec...
[M::process] read 672722 sequences (100000192 bp) in 1.160 CPU sec, 1.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284604, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.17, 142.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 86, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (178.60, 235.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673170 reads in 175.819 CPU sec, 5.628 real sec
[M::mem_process_seqs] Processed 673092 reads in 98.956 CPU sec, 3.170 real sec
sorting block 43, #reads = 1079553 mem = 536881180 peak = 956571648
[M::process] read 672624 sequences (100000148 bp) in 1.975 CPU sec, 2.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284456, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.06, 145.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672682 sequences (100000243 bp) in 1.170 CPU sec, 1.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283652, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.19, 150.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283657, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (288.22, 153.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 691, 722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1698)
[M::mem_pestat] mean and std.dev: (463.73, 305.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2186)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673006 reads in 262.994 CPU sec, 8.438 real sec
[M::mem_process_seqs] Processed 672722 reads in 184.147 CPU sec, 5.941 real sec
[M::mem_process_seqs] Processed 672624 reads in 191.767 CPU sec, 6.212 real sec
sorting block 44, #reads = 1079590 mem = 536878449 peak = 956571648
sorting block 45, #reads = 1078610 mem = 536882524 peak = 956571648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283703, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (286.49, 152.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 203, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (154.42, 105.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 906)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672876 sequences (100000031 bp) in 2.520 CPU sec, 2.924 real sec...
[M::mem_process_seqs] Processed 672682 reads in 96.983 CPU sec, 3.193 real sec
sorting block 46, #reads = 1078992 mem = 536879836 peak = 956571648
[M::process] read 673000 sequences (100000236 bp) in 2.442 CPU sec, 2.886 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284663, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.13, 150.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673422 sequences (100000232 bp) in 1.159 CPU sec, 1.162 real sec...
[M::process] read 673384 sequences (100000175 bp) in 1.129 CPU sec, 1.126 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283882, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.43, 144.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (81, 81, 188)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402)
[M::mem_pestat] mean and std.dev: (115.43, 100.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 516)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672876 reads in 179.414 CPU sec, 5.714 real sec
[M::mem_process_seqs] Processed 673000 reads in 102.291 CPU sec, 3.272 real sec
sorting block 47, #reads = 1079813 mem = 536879076 peak = 956571648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284871, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.22, 136.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673230 sequences (100000174 bp) in 2.418 CPU sec, 2.820 real sec...
[M::process] read 673142 sequences (100000269 bp) in 1.159 CPU sec, 1.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285155, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.34, 139.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 114, 653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1911)
[M::mem_pestat] mean and std.dev: (258.83, 282.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2540)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673422 reads in 178.425 CPU sec, 5.713 real sec
[M::mem_process_seqs] Processed 673384 reads in 99.959 CPU sec, 3.204 real sec
sorting block 48, #reads = 1080091 mem = 536878530 peak = 956571648
[M::process] read 673056 sequences (100000082 bp) in 1.906 CPU sec, 2.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285034, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.54, 141.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 96, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1673)
[M::mem_pestat] mean and std.dev: (167.89, 219.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2230)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673066 sequences (100000100 bp) in 1.200 CPU sec, 1.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284310, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.65, 146.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285011, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.76, 144.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (252, 392, 800)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1896)
[M::mem_pestat] mean and std.dev: (412.08, 304.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2444)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673230 reads in 257.877 CPU sec, 8.277 real sec
[M::mem_process_seqs] Processed 673056 reads in 181.402 CPU sec, 5.858 real sec
[M::mem_process_seqs] Processed 673142 reads in 189.181 CPU sec, 6.136 real sec
sorting block 49, #reads = 1079841 mem = 536879559 peak = 956571648
[M::process] read 673406 sequences (100000219 bp) in 1.916 CPU sec, 2.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284446, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (276.30, 148.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 50, #reads = 1079508 mem = 536879599 peak = 956571648
[M::process] read 673160 sequences (100000283 bp) in 2.106 CPU sec, 2.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284846, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.07, 141.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673102 sequences (100000210 bp) in 1.170 CPU sec, 1.169 real sec...
[M::mem_process_seqs] Processed 673066 reads in 181.581 CPU sec, 5.832 real sec
[M::mem_process_seqs] Processed 673406 reads in 103.186 CPU sec, 3.301 real sec
sorting block 51, #reads = 1079534 mem = 536878936 peak = 956571648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285104, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.14, 141.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673098 sequences (100000253 bp) in 2.457 CPU sec, 2.877 real sec...
[M::process] read 673156 sequences (100000053 bp) in 1.140 CPU sec, 1.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284824, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.73, 139.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673160 reads in 176.954 CPU sec, 5.667 real sec
[M::mem_process_seqs] Processed 673102 reads in 100.764 CPU sec, 3.242 real sec
sorting block 52, #reads = 1079556 mem = 536879326 peak = 956571648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284310, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.49, 139.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 92, 92)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240)
[M::mem_pestat] mean and std.dev: (72.10, 54.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 314)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672862 sequences (100000000 bp) in 2.223 CPU sec, 2.516 real sec...
[M::process] read 672738 sequences (100000103 bp) in 1.140 CPU sec, 1.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284766, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (274.84, 143.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 6, 39)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 105)
[M::mem_pestat] mean and std.dev: (19.40, 26.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 138)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673098 reads in 179.426 CPU sec, 5.747 real sec
sorting block 53, #reads = 1079424 mem = 536879978 peak = 958894080
[M::mem_process_seqs] Processed 673156 reads in 101.825 CPU sec, 3.279 real sec
sorting block 54, #reads = 1079794 mem = 536878839 peak = 1045504000
[M::process] read 672666 sequences (100000295 bp) in 2.020 CPU sec, 2.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283795, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.45, 148.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672904 sequences (100000244 bp) in 1.120 CPU sec, 1.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283171, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (288.36, 154.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283677, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (286.87, 152.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 130, 618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1804)
[M::mem_pestat] mean and std.dev: (258.83, 278.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2397)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672862 reads in 274.167 CPU sec, 8.810 real sec
[M::mem_process_seqs] Processed 672738 reads in 193.104 CPU sec, 6.246 real sec
[M::mem_process_seqs] Processed 672666 reads in 201.485 CPU sec, 6.544 real sec
sorting block 55, #reads = 1079225 mem = 536878976 peak = 1045504000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283475, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (280.41, 149.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673238 sequences (100000192 bp) in 2.747 CPU sec, 3.204 real sec...
[M::mem_process_seqs] Processed 672904 reads in 104.358 CPU sec, 3.447 real sec
sorting block 56, #reads = 1079035 mem = 536876462 peak = 1045504000
[M::process] read 673656 sequences (100000041 bp) in 2.573 CPU sec, 3.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284537, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.91, 142.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (99, 212, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1749)
[M::mem_pestat] mean and std.dev: (271.92, 259.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2299)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673356 sequences (100000253 bp) in 1.213 CPU sec, 1.230 real sec...
[M::process] read 673162 sequences (100000067 bp) in 1.140 CPU sec, 1.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284645, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.38, 130.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673238 reads in 184.567 CPU sec, 5.895 real sec
[M::mem_process_seqs] Processed 673656 reads in 101.521 CPU sec, 3.242 real sec
sorting block 57, #reads = 1079096 mem = 536880003 peak = 1045504000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284465, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (267.90, 140.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1080237 mem = 536878649 peak = 1045504000
[M::process] read 673140 sequences (100000207 bp) in 2.553 CPU sec, 2.894 real sec...
[M::process] read 673034 sequences (100000240 bp) in 1.290 CPU sec, 1.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283932, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.52, 142.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 11, 25)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 69)
[M::mem_pestat] mean and std.dev: (13.00, 11.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 91)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673356 reads in 182.880 CPU sec, 5.845 real sec
[M::mem_process_seqs] Processed 673162 reads in 102.196 CPU sec, 3.272 real sec
sorting block 59, #reads = 1079922 mem = 536879245 peak = 1045504000
[M::process] read 673186 sequences (100000114 bp) in 1.772 CPU sec, 1.981 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284099, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.64, 144.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 40, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252)
[M::mem_pestat] mean and std.dev: (44.50, 37.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 335)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673292 sequences (100000034 bp) in 1.190 CPU sec, 1.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284556, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.63, 148.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 86, 122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354)
[M::mem_pestat] mean and std.dev: (57.55, 48.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 470)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284098, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (273.85, 147.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 130, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1959)
[M::mem_pestat] mean and std.dev: (274.00, 337.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2608)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673140 reads in 261.726 CPU sec, 8.413 real sec
[M::mem_process_seqs] Processed 673034 reads in 183.148 CPU sec, 5.923 real sec
[M::mem_process_seqs] Processed 673186 reads in 191.640 CPU sec, 6.226 real sec
sorting block 60, #reads = 1079262 mem = 536878200 peak = 1045504000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284932, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.64, 138.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673292 reads in 97.450 CPU sec, 3.220 real sec
[M::process] read 673274 sequences (100000132 bp) in 2.795 CPU sec, 3.224 real sec...
sorting block 61, #reads = 1079357 mem = 536876061 peak = 1045504000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285026, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.58, 139.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 87, 2365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7083)
[M::mem_pestat] mean and std.dev: (1084.00, 1626.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9442)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673288 sequences (100000278 bp) in 2.406 CPU sec, 2.855 real sec...
[M::mem_process_seqs] Processed 673274 reads in 96.109 CPU sec, 3.138 real sec
sorting block 62, #reads = 1078955 mem = 536880743 peak = 1045504000
[M::process] read 673426 sequences (100000132 bp) in 2.400 CPU sec, 2.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284563, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.67, 137.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 4462, 4462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13304)
[M::mem_pestat] mean and std.dev: (2269.50, 2193.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17725)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673340 sequences (100000241 bp) in 1.120 CPU sec, 1.127 real sec...
[M::process] read 673068 sequences (100000109 bp) in 1.130 CPU sec, 1.126 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284589, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.74, 135.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673288 reads in 181.875 CPU sec, 5.777 real sec
[M::mem_process_seqs] Processed 673426 reads in 104.189 CPU sec, 3.343 real sec
sorting block 63, #reads = 1079464 mem = 536879394 peak = 1045504000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284282, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.57, 137.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672850 sequences (100000106 bp) in 2.065 CPU sec, 2.312 real sec...
[M::process] read 672936 sequences (100000126 bp) in 1.076 CPU sec, 1.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283848, 24, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (273.41, 142.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 217, 2729)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8137)
[M::mem_pestat] mean and std.dev: (1187.92, 1966.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10841)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673340 reads in 183.893 CPU sec, 5.904 real sec
[M::mem_process_seqs] Processed 673068 reads in 100.209 CPU sec, 3.232 real sec
sorting block 64, #reads = 1079765 mem = 536878413 peak = 1045504000
[M::process] read 673000 sequences (100000066 bp) in 1.777 CPU sec, 1.981 real sec...
[M::process] read 673156 sequences (100000139 bp) in 1.120 CPU sec, 1.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283604, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.41, 149.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 6, 98)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286)
[M::mem_pestat] mean and std.dev: (16.47, 29.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 380)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672936 reads in 102.230 CPU sec, 3.295 real sec
sorting block 65, #reads = 1079019 mem = 536878127 peak = 1045504000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283924, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.95, 147.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673556 sequences (100000070 bp) in 1.448 CPU sec, 1.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284392, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (279.83, 148.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 25, 44)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 92)
[M::mem_pestat] mean and std.dev: (28.60, 13.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 116)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672850 reads in 284.270 CPU sec, 9.109 real sec
[M::mem_process_seqs] Processed 673000 reads in 202.235 CPU sec, 6.514 real sec
sorting block 66, #reads = 1079594 mem = 536878028 peak = 1064460288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284197, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.39, 139.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673306 sequences (100000065 bp) in 2.458 CPU sec, 2.725 real sec...
[M::process] read 673268 sequences (100000203 bp) in 1.670 CPU sec, 1.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284833, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.87, 132.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (92, 619, 8060)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23996)
[M::mem_pestat] mean and std.dev: (2683.40, 3534.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 31964)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673156 reads in 185.264 CPU sec, 5.925 real sec
sorting block 67, #reads = 1079555 mem = 536878754 peak = 1064460288
[M::mem_process_seqs] Processed 673556 reads in 102.114 CPU sec, 3.269 real sec
sorting block 68, #reads = 1079783 mem = 536880454 peak = 1064460288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284692, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.74, 141.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 31, 50)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 144)
[M::mem_pestat] mean and std.dev: (30.64, 31.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 191)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673082 sequences (100000238 bp) in 2.722 CPU sec, 3.034 real sec...
[M::process] read 673052 sequences (100000079 bp) in 1.580 CPU sec, 1.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284566, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.37, 142.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (49, 224, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (162.60, 89.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 749)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673306 reads in 183.423 CPU sec, 5.852 real sec
[M::mem_process_seqs] Processed 673268 reads in 102.809 CPU sec, 3.293 real sec
sorting block 69, #reads = 1079932 mem = 536878051 peak = 1064460288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285280, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.49, 145.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673040 sequences (100000158 bp) in 2.165 CPU sec, 2.401 real sec...
[M::process] read 673588 sequences (100000128 bp) in 1.360 CPU sec, 1.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284872, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (275.84, 148.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673082 reads in 177.998 CPU sec, 5.695 real sec
[M::mem_process_seqs] Processed 673052 reads in 99.391 CPU sec, 3.191 real sec
sorting block 70, #reads = 1079819 mem = 536876904 peak = 1064460288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285152, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (277.87, 150.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673566 sequences (100000110 bp) in 1.931 CPU sec, 2.103 real sec...
[M::process] read 673522 sequences (100000207 bp) in 1.250 CPU sec, 1.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285172, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.53, 137.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673040 reads in 168.318 CPU sec, 5.386 real sec
[M::mem_process_seqs] Processed 673588 reads in 96.717 CPU sec, 3.104 real sec
sorting block 71, #reads = 1078861 mem = 536881029 peak = 1064460288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285405, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (263.97, 135.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673484 sequences (100000198 bp) in 2.056 CPU sec, 2.339 real sec...
[M::process] read 673312 sequences (100000215 bp) in 1.309 CPU sec, 1.310 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285341, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.03, 132.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673566 reads in 174.541 CPU sec, 5.588 real sec
[M::mem_process_seqs] Processed 673522 reads in 98.875 CPU sec, 3.169 real sec
sorting block 72, #reads = 1078428 mem = 536878486 peak = 1064460288
sorting block 73, #reads = 1079589 mem = 536880569 peak = 1081335808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285037, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.11, 134.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673264 sequences (100000168 bp) in 1.909 CPU sec, 2.207 real sec...
[M::process] read 673006 sequences (100000268 bp) in 1.070 CPU sec, 1.068 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284402, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.11, 136.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 15, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156)
[M::mem_pestat] mean and std.dev: (132.36, 169.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1538)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673484 reads in 178.025 CPU sec, 5.712 real sec
[M::mem_process_seqs] Processed 673312 reads in 101.943 CPU sec, 3.293 real sec
sorting block 74, #reads = 1079115 mem = 536883135 peak = 1081335808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284310, 13, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.06, 138.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (67, 199, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (238.00, 187.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1347)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673092 sequences (100000012 bp) in 2.046 CPU sec, 2.319 real sec...
[M::process] read 673082 sequences (100000262 bp) in 1.130 CPU sec, 1.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283572, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (274.65, 143.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673264 reads in 186.677 CPU sec, 5.990 real sec
[M::mem_process_seqs] Processed 673006 reads in 106.161 CPU sec, 3.428 real sec
sorting block 75, #reads = 1079584 mem = 536877847 peak = 1081335808
[M::process] read 673392 sequences (100000232 bp) in 1.898 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284307, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (274.01, 143.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673536 sequences (100000002 bp) in 1.090 CPU sec, 1.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283957, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.70, 144.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284776, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.38, 137.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673092 reads in 268.866 CPU sec, 8.645 real sec
[M::mem_process_seqs] Processed 673082 reads in 187.767 CPU sec, 6.070 real sec
[M::mem_process_seqs] Processed 673392 reads in 196.725 CPU sec, 6.391 real sec
sorting block 76, #reads = 1079382 mem = 536877443 peak = 1081335808
sorting block 77, #reads = 1079419 mem = 536879755 peak = 1081335808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284974, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.14, 134.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673262 sequences (100000151 bp) in 2.671 CPU sec, 3.154 real sec...
[M::mem_process_seqs] Processed 673536 reads in 97.470 CPU sec, 3.216 real sec
sorting block 78, #reads = 1079741 mem = 536877044 peak = 1081335808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284613, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 673040 sequences (100000173 bp) in 2.453 CPU sec, 2.929 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.81, 140.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 122, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (217.24, 221.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673008 sequences (100000219 bp) in 1.100 CPU sec, 1.100 real sec...
[M::process] read 673020 sequences (100000061 bp) in 1.080 CPU sec, 1.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284314, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.36, 142.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673262 reads in 182.163 CPU sec, 5.859 real sec
[M::mem_process_seqs] Processed 673040 reads in 102.543 CPU sec, 3.278 real sec
sorting block 79, #reads = 1079441 mem = 536878084 peak = 1081335808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284252, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (276.21, 146.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673030 sequences (100000260 bp) in 2.635 CPU sec, 2.916 real sec...
[M::process] read 95646 sequences (14211070 bp) in 0.480 CPU sec, 0.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284305, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (280.72, 151.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673008 reads in 185.100 CPU sec, 5.947 real sec
[M::mem_process_seqs] Processed 673020 reads in 102.882 CPU sec, 3.328 real sec
sorting block 80, #reads = 1079837 mem = 536880345 peak = 1081335808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284124, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (279.54, 152.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 86, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077)
[M::mem_pestat] mean and std.dev: (223.90, 252.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1413)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 40493, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (277.60, 151.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673030 reads in 107.485 CPU sec, 3.509 real sec
[M::mem_process_seqs] Processed 95646 reads in 21.528 CPU sec, 0.709 real sec
sorting block 81, #reads = 1079056 mem = 536880075 peak = 1081335808
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 88266318
overall: 69632278528 mem 13969.557 user 726.485 sys 921.432 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L004\tPU:L004\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.T18-D0003255_R1.fastq.gz 0004.T18-D0003255_R2.fastq.gz
[main] Real time: 921.457 sec; CPU: 14696.045 sec
sorting block 82, #reads = 375949 mem = 186924401 peak = 1081335808
uniq header count 1
merging 83 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 3370213721
output reads: 88899638
bam_mem_sort: 83 calls 35.286 user 0.560 sys 36.005 real
bam_write: 83 calls 22.161 user 1.034 sys 23.406 real
execute: 1 calls 16.951 user 0.942 sys 941.128 real
merge_files: 1 calls 16.926 user 0.910 sys 19.252 real
parse_chunk: 1318 calls 48.859 user 13.132 sys 63.202 real
read_chunk: 3547 calls 33.504 user 29.004 sys 421.145 real
sort_block: 1 calls 26.080 user 28.422 sys 409.533 real
write_chunk: 2227 calls 16.609 user 0.671 sys 18.003 real
overall: 2471833600 mem 756.387 user 58.808 sys 941.147 real
ls: cannot access '*.cram': No such file or directory