Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/45ef45b79bfc6d039515cb48e10c85/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/94/29a8cbe3c3c4d2b262932cb234c4b5/T18-D0003255/0002.T18-D0003255_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/94/29a8cbe3c3c4d2b262932cb234c4b5/T18-D0003255/0002.T18-D0003255_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/45ef45b79bfc6d039515cb48e10c85/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
No more license available for sentieon:klib
No more license available for sentieon:klib
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 2959.628 CPU sec, 221.514 real sec
[M::process] read 673614 sequences (100000152 bp) in 1.053 CPU sec, 1.062 real sec...
[M::process] read 673490 sequences (100000150 bp) in 1.540 CPU sec, 1.531 real sec...
[M::process] read 673514 sequences (100000239 bp) in 1.548 CPU sec, 1.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286129, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (261.90, 130.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673520 sequences (100000266 bp) in 1.551 CPU sec, 1.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286026, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.78, 131.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285662, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (260.41, 129.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673614 reads in 373.097 CPU sec, 12.009 real sec
[M::mem_process_seqs] Processed 673490 reads in 254.230 CPU sec, 8.284 real sec
[M::mem_process_seqs] Processed 673514 reads in 263.502 CPU sec, 8.614 real sec
sorting block 0, #reads = 1079873 mem = 536877433 peak = 590643200
[M::process] read 673516 sequences (100000189 bp) in 2.357 CPU sec, 2.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285442, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (260.51, 129.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673350 sequences (100000216 bp) in 1.907 CPU sec, 2.002 real sec...
[M::process] read 673328 sequences (100000135 bp) in 1.469 CPU sec, 1.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285301, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.41, 132.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673520 reads in 247.956 CPU sec, 7.911 real sec
sorting block 1, #reads = 1080151 mem = 536879025 peak = 804552704
[M::mem_process_seqs] Processed 673516 reads in 135.495 CPU sec, 4.337 real sec
sorting block 2, #reads = 1080275 mem = 536880695 peak = 902098944
[M::process] read 673270 sequences (100000289 bp) in 3.326 CPU sec, 3.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285329, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.81, 134.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673372 sequences (100000018 bp) in 1.980 CPU sec, 1.978 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285017, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.95, 135.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285564, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.44, 136.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673350 reads in 358.094 CPU sec, 11.443 real sec
[M::mem_process_seqs] Processed 673328 reads in 246.100 CPU sec, 7.892 real sec
[M::mem_process_seqs] Processed 673270 reads in 255.257 CPU sec, 8.212 real sec
sorting block 3, #reads = 1079362 mem = 536876748 peak = 902098944
[M::process] read 673312 sequences (100000106 bp) in 3.512 CPU sec, 3.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285147, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.58, 134.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 18, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193)
[M::mem_pestat] mean and std.dev: (31.45, 31.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 253)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673372 reads in 130.159 CPU sec, 4.255 real sec
sorting block 4, #reads = 1080226 mem = 536881033 peak = 902098944
[M::process] read 673188 sequences (100000232 bp) in 3.178 CPU sec, 3.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285149, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.42, 136.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673096 sequences (100000281 bp) in 1.960 CPU sec, 1.953 real sec...
[M::process] read 672922 sequences (100000151 bp) in 2.110 CPU sec, 2.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284653, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.79, 138.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 185, 1192)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3524)
[M::mem_pestat] mean and std.dev: (397.58, 496.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4690)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673312 reads in 252.743 CPU sec, 8.017 real sec
[M::mem_process_seqs] Processed 673188 reads in 142.431 CPU sec, 4.537 real sec
sorting block 5, #reads = 1079447 mem = 536880333 peak = 902098944
[M::process] read 672940 sequences (100000276 bp) in 2.549 CPU sec, 2.801 real sec...
[M::process] read 672720 sequences (100000104 bp) in 1.380 CPU sec, 1.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284412, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.36, 142.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672922 reads in 127.361 CPU sec, 4.075 real sec
sorting block 6, #reads = 1079542 mem = 536879667 peak = 902098944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284139, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.82, 140.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672700 sequences (100000280 bp) in 1.656 CPU sec, 1.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284489, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.96, 144.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673096 reads in 370.800 CPU sec, 11.859 real sec
[M::mem_process_seqs] Processed 672940 reads in 255.988 CPU sec, 8.190 real sec
sorting block 7, #reads = 1079359 mem = 536878367 peak = 902098944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284226, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.27, 145.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672918 sequences (100000164 bp) in 3.507 CPU sec, 3.774 real sec...
[M::process] read 673522 sequences (100000054 bp) in 1.890 CPU sec, 1.890 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284836, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (283.12, 149.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 331, 4885)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14509)
[M::mem_pestat] mean and std.dev: (2648.86, 3544.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19321)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672720 reads in 242.189 CPU sec, 7.707 real sec
[M::mem_process_seqs] Processed 672700 reads in 129.422 CPU sec, 4.122 real sec
sorting block 8, #reads = 1079703 mem = 536879140 peak = 902098944
sorting block 9, #reads = 1078936 mem = 536880086 peak = 1004433408
[M::process] read 673342 sequences (100000112 bp) in 2.024 CPU sec, 2.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285300, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.25, 143.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 130, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1885)
[M::mem_pestat] mean and std.dev: (299.36, 309.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2503)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673462 sequences (100000000 bp) in 2.060 CPU sec, 2.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285648, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.92, 134.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 757, 773)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1949)
[M::mem_pestat] mean and std.dev: (800.00, 632.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3328)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286289, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.40, 135.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 90, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1458)
[M::mem_pestat] mean and std.dev: (259.38, 378.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1938)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672918 reads in 336.272 CPU sec, 10.783 real sec
[M::mem_process_seqs] Processed 673342 reads in 231.553 CPU sec, 7.447 real sec
[M::mem_process_seqs] Processed 673522 reads in 239.738 CPU sec, 7.732 real sec
sorting block 10, #reads = 1079675 mem = 536878846 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285935, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (261.55, 131.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673462 reads in 131.176 CPU sec, 4.323 real sec
[M::process] read 673518 sequences (100000134 bp) in 4.357 CPU sec, 4.775 real sec...
sorting block 11, #reads = 1080444 mem = 536879359 peak = 1004433408
[M::process] read 673346 sequences (100000144 bp) in 2.786 CPU sec, 3.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285280, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.73, 133.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673366 sequences (100000172 bp) in 1.920 CPU sec, 1.918 real sec...
[M::process] read 673326 sequences (100000249 bp) in 1.300 CPU sec, 1.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285425, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.31, 135.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673518 reads in 240.886 CPU sec, 7.630 real sec
[M::mem_process_seqs] Processed 673346 reads in 131.623 CPU sec, 4.203 real sec
sorting block 12, #reads = 1080368 mem = 536878838 peak = 1004433408
[M::process] read 673318 sequences (100000215 bp) in 2.875 CPU sec, 3.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285369, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.22, 136.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673350 sequences (100000274 bp) in 1.880 CPU sec, 1.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285494, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.15, 135.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 224, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1853)
[M::mem_pestat] mean and std.dev: (328.75, 399.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2455)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285298, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.37, 138.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 17, 721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2155)
[M::mem_pestat] mean and std.dev: (220.07, 317.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2872)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673366 reads in 350.031 CPU sec, 11.207 real sec
[M::mem_process_seqs] Processed 673318 reads in 241.728 CPU sec, 7.765 real sec
sorting block 13, #reads = 1080230 mem = 536877338 peak = 1004433408
[M::mem_process_seqs] Processed 673326 reads in 249.855 CPU sec, 8.044 real sec
sorting block 14, #reads = 1080039 mem = 536879113 peak = 1004433408
[M::process] read 673332 sequences (100000086 bp) in 2.752 CPU sec, 2.959 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285083, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.19, 135.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 46, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1714)
[M::mem_pestat] mean and std.dev: (366.00, 506.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2392)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673148 sequences (100000164 bp) in 1.978 CPU sec, 1.994 real sec...
[M::process] read 672988 sequences (100000241 bp) in 1.690 CPU sec, 1.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284842, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.56, 138.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673350 reads in 243.884 CPU sec, 7.795 real sec
[M::mem_process_seqs] Processed 673332 reads in 131.129 CPU sec, 4.163 real sec
sorting block 15, #reads = 1080410 mem = 536878626 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285366, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.04, 141.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 69, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1959)
[M::mem_pestat] mean and std.dev: (288.60, 321.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2608)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672980 sequences (100000196 bp) in 4.120 CPU sec, 4.502 real sec...
[M::process] read 672828 sequences (100000225 bp) in 1.500 CPU sec, 1.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284459, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.60, 142.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 224, 1841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5263)
[M::mem_pestat] mean and std.dev: (590.11, 689.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6974)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673148 reads in 244.864 CPU sec, 7.815 real sec
[M::mem_process_seqs] Processed 672988 reads in 135.605 CPU sec, 4.312 real sec
sorting block 16, #reads = 1079964 mem = 536878223 peak = 1004433408
sorting block 17, #reads = 1080019 mem = 536878923 peak = 1004433408
[M::process] read 672830 sequences (100000277 bp) in 2.422 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284831, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (274.87, 143.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672676 sequences (100000142 bp) in 2.080 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285093, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.51, 146.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 40, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (195.36, 264.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1377)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284926, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.01, 148.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672980 reads in 360.937 CPU sec, 11.570 real sec
[M::mem_process_seqs] Processed 672828 reads in 247.243 CPU sec, 7.953 real sec
[M::mem_process_seqs] Processed 672830 reads in 255.762 CPU sec, 8.245 real sec
sorting block 18, #reads = 1080027 mem = 536879705 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285099, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.19, 149.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672676 reads in 130.088 CPU sec, 4.280 real sec
[M::process] read 673338 sequences (100000209 bp) in 4.109 CPU sec, 4.512 real sec...
sorting block 19, #reads = 1079620 mem = 536879155 peak = 1004433408
[M::process] read 673078 sequences (100000064 bp) in 2.587 CPU sec, 2.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286223, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.76, 135.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673322 sequences (100000050 bp) in 1.800 CPU sec, 1.807 real sec...
[M::process] read 673392 sequences (100000159 bp) in 1.810 CPU sec, 1.807 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285709, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.98, 136.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673338 reads in 235.989 CPU sec, 7.495 real sec
[M::mem_process_seqs] Processed 673078 reads in 129.261 CPU sec, 4.121 real sec
sorting block 20, #reads = 1079193 mem = 536878958 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285749, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.06, 134.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 86, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (69.67, 76.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673396 sequences (100000023 bp) in 3.653 CPU sec, 3.941 real sec...
[M::process] read 673162 sequences (100000078 bp) in 1.890 CPU sec, 1.894 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286350, 14, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.54, 133.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 84, 698)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2004)
[M::mem_pestat] mean and std.dev: (294.00, 355.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2657)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673322 reads in 238.701 CPU sec, 7.615 real sec
sorting block 21, #reads = 1079728 mem = 536881939 peak = 1004433408
[M::mem_process_seqs] Processed 673392 reads in 129.431 CPU sec, 4.103 real sec
sorting block 22, #reads = 1080367 mem = 536877882 peak = 1004433408
[M::process] read 673292 sequences (100000274 bp) in 2.367 CPU sec, 2.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285940, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.23, 135.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 41, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (86.00, 110.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673314 sequences (100000162 bp) in 2.030 CPU sec, 2.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285058, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.30, 137.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 191, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (122.08, 88.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285387, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.33, 138.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673396 reads in 352.720 CPU sec, 11.308 real sec
[M::mem_process_seqs] Processed 673162 reads in 245.336 CPU sec, 7.881 real sec
[M::mem_process_seqs] Processed 673292 reads in 253.742 CPU sec, 8.171 real sec
sorting block 23, #reads = 1080117 mem = 536876846 peak = 1004433408
[M::process] read 673494 sequences (100000094 bp) in 2.798 CPU sec, 3.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285165, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.47, 138.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 106, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (145.10, 159.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 24, #reads = 1080445 mem = 536877564 peak = 1004433408
[M::process] read 673298 sequences (100000224 bp) in 2.736 CPU sec, 2.827 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285545, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.34, 134.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673314 reads in 243.745 CPU sec, 7.763 real sec
[M::process] read 673180 sequences (100000039 bp) in 1.950 CPU sec, 1.953 real sec...
[M::mem_process_seqs] Processed 673494 reads in 133.486 CPU sec, 4.208 real sec
sorting block 25, #reads = 1079974 mem = 536879367 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284799, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.26, 138.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 37, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (79.25, 107.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673078 sequences (100000061 bp) in 3.500 CPU sec, 3.801 real sec...
[M::process] read 672996 sequences (100000009 bp) in 2.000 CPU sec, 1.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284597, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.01, 139.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 41, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103)
[M::mem_pestat] mean and std.dev: (103.18, 138.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1461)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673298 reads in 247.798 CPU sec, 7.901 real sec
[M::mem_process_seqs] Processed 673180 reads in 135.866 CPU sec, 4.319 real sec
sorting block 26, #reads = 1079849 mem = 536879831 peak = 1004433408
[M::process] read 672828 sequences (100000263 bp) in 2.291 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284666, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (273.07, 141.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 181, 1164)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3296)
[M::mem_pestat] mean and std.dev: (438.10, 476.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4362)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672718 sequences (100000148 bp) in 1.960 CPU sec, 1.959 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284991, 16, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.81, 141.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 43, 985)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2923)
[M::mem_pestat] mean and std.dev: (627.94, 1079.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4947)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284973, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.94, 145.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673078 reads in 361.652 CPU sec, 11.605 real sec
[M::mem_process_seqs] Processed 672996 reads in 251.335 CPU sec, 8.092 real sec
[M::mem_process_seqs] Processed 672828 reads in 260.279 CPU sec, 8.401 real sec
sorting block 27, #reads = 1079270 mem = 536879180 peak = 1004433408
sorting block 28, #reads = 1079659 mem = 536878574 peak = 1004433408
[M::process] read 672678 sequences (100000013 bp) in 3.352 CPU sec, 3.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285167, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.13, 146.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 18, 65)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 159)
[M::mem_pestat] mean and std.dev: (37.89, 45.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 219)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673016 sequences (100000044 bp) in 2.210 CPU sec, 2.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285060, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.37, 149.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673232 sequences (100000011 bp) in 2.000 CPU sec, 2.000 real sec...
[M::mem_process_seqs] Processed 672718 reads in 251.634 CPU sec, 8.024 real sec
[M::mem_process_seqs] Processed 672678 reads in 137.311 CPU sec, 4.330 real sec
sorting block 29, #reads = 1079990 mem = 536880672 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285594, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (272.87, 140.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (55, 91, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 196)
[M::mem_pestat] mean and std.dev: (65.83, 29.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 243)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673116 sequences (100000254 bp) in 3.596 CPU sec, 3.941 real sec...
[M::process] read 673374 sequences (100000009 bp) in 2.200 CPU sec, 2.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286587, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.30, 134.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 81, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142)
[M::mem_pestat] mean and std.dev: (219.15, 260.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1506)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673016 reads in 238.282 CPU sec, 7.588 real sec
[M::mem_process_seqs] Processed 673232 reads in 130.986 CPU sec, 4.151 real sec
sorting block 30, #reads = 1079515 mem = 536876877 peak = 1004433408
sorting block 31, #reads = 1080026 mem = 536880865 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285766, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (268.01, 135.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673390 sequences (100000017 bp) in 2.932 CPU sec, 3.150 real sec...
[M::process] read 673302 sequences (100000116 bp) in 2.230 CPU sec, 2.229 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286184, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.93, 134.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673116 reads in 237.708 CPU sec, 7.578 real sec
[M::mem_process_seqs] Processed 673374 reads in 129.750 CPU sec, 4.123 real sec
sorting block 32, #reads = 1080235 mem = 536876279 peak = 1004433408
[M::process] read 673248 sequences (100000106 bp) in 2.978 CPU sec, 3.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285798, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.96, 133.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673110 sequences (100000108 bp) in 1.890 CPU sec, 1.885 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286121, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.15, 136.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673390 reads in 237.887 CPU sec, 7.597 real sec
[M::mem_process_seqs] Processed 673302 reads in 129.922 CPU sec, 4.146 real sec
sorting block 33, #reads = 1080229 mem = 536877189 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285592, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (267.04, 135.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673242 sequences (100000283 bp) in 2.955 CPU sec, 3.166 real sec...
[M::process] read 673218 sequences (100000171 bp) in 2.270 CPU sec, 2.268 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284907, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.00, 138.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 86, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248)
[M::mem_pestat] mean and std.dev: (139.21, 180.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1641)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673248 reads in 242.570 CPU sec, 7.737 real sec
[M::mem_process_seqs] Processed 673110 reads in 134.489 CPU sec, 4.286 real sec
sorting block 34, #reads = 1080101 mem = 536879853 peak = 1004433408
[M::process] read 673250 sequences (100000002 bp) in 2.956 CPU sec, 3.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285749, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.82, 136.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 196, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1847)
[M::mem_pestat] mean and std.dev: (188.36, 207.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2449)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673188 sequences (100000294 bp) in 2.250 CPU sec, 2.254 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285569, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.90, 135.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285176, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.29, 136.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673242 reads in 359.079 CPU sec, 11.492 real sec
[M::mem_process_seqs] Processed 673218 reads in 249.261 CPU sec, 8.005 real sec
sorting block 35, #reads = 1079235 mem = 536879961 peak = 1004433408
[M::mem_process_seqs] Processed 673250 reads in 257.609 CPU sec, 8.295 real sec
sorting block 36, #reads = 1080117 mem = 536882481 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285258, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.55, 138.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 367, 1051)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2925)
[M::mem_pestat] mean and std.dev: (369.64, 345.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3862)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673188 reads in 130.580 CPU sec, 4.303 real sec
[M::process] read 672912 sequences (100000060 bp) in 4.133 CPU sec, 4.555 real sec...
sorting block 37, #reads = 1079769 mem = 536879057 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284523, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.20, 139.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672912 reads in 130.708 CPU sec, 4.274 real sec
[M::process] read 672862 sequences (100000034 bp) in 4.200 CPU sec, 4.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284759, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.30, 142.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 67, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (72.11, 54.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672834 sequences (100000214 bp) in 3.827 CPU sec, 4.149 real sec...
[M::mem_process_seqs] Processed 672862 reads in 129.929 CPU sec, 4.174 real sec
sorting block 38, #reads = 1079518 mem = 536881743 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285031, 19, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.24, 143.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 185, 783)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2175)
[M::mem_pestat] mean and std.dev: (374.84, 503.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2871)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672834 reads in 128.589 CPU sec, 4.131 real sec
[M::process] read 672794 sequences (100000045 bp) in 4.016 CPU sec, 4.393 real sec...
sorting block 39, #reads = 1079709 mem = 536878833 peak = 1004433408
[M::process] read 672716 sequences (100000075 bp) in 3.149 CPU sec, 3.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285302, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.69, 143.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673610 sequences (100000238 bp) in 2.060 CPU sec, 2.059 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285544, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 204, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (281.51, 146.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673558 sequences (100000262 bp) in 1.890 CPU sec, 1.896 real sec...
[M::mem_process_seqs] Processed 672794 reads in 239.038 CPU sec, 7.542 real sec
[M::mem_process_seqs] Processed 672716 reads in 130.645 CPU sec, 4.134 real sec
sorting block 40, #reads = 1079930 mem = 536879655 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285319, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.19, 137.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (103, 167, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (234.70, 204.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673762 sequences (100000163 bp) in 2.838 CPU sec, 3.053 real sec...
[M::process] read 673584 sequences (100000116 bp) in 1.920 CPU sec, 1.915 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285705, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.57, 135.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 239, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208)
[M::mem_pestat] mean and std.dev: (333.73, 294.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1549)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673610 reads in 235.752 CPU sec, 7.522 real sec
[M::mem_process_seqs] Processed 673558 reads in 129.782 CPU sec, 4.143 real sec
sorting block 41, #reads = 1079715 mem = 536879280 peak = 1004433408
[M::process] read 673598 sequences (100000032 bp) in 3.163 CPU sec, 3.349 real sec...
[M::process] read 673608 sequences (100000106 bp) in 2.258 CPU sec, 2.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284942, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (266.02, 137.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673598 reads in 138.276 CPU sec, 4.413 real sec
sorting block 42, #reads = 1078855 mem = 536878737 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285069, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.37, 133.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673472 sequences (100000195 bp) in 1.913 CPU sec, 1.916 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285007, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.44, 136.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (81, 238, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (276.53, 186.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673762 reads in 376.256 CPU sec, 11.994 real sec
[M::mem_process_seqs] Processed 673584 reads in 262.996 CPU sec, 8.394 real sec
sorting block 43, #reads = 1079256 mem = 536879864 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285581, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.64, 136.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 29, 81)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229)
[M::mem_pestat] mean and std.dev: (30.78, 30.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 303)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673404 sequences (100000022 bp) in 3.082 CPU sec, 3.324 real sec...
[M::process] read 673384 sequences (100000035 bp) in 1.960 CPU sec, 1.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285333, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.52, 136.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 569, 569)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1525)
[M::mem_pestat] mean and std.dev: (372.64, 260.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2003)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673608 reads in 238.043 CPU sec, 7.612 real sec
[M::mem_process_seqs] Processed 673472 reads in 134.734 CPU sec, 4.281 real sec
sorting block 44, #reads = 1078017 mem = 536877790 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285440, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.02, 135.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 122, 122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340)
[M::mem_pestat] mean and std.dev: (70.70, 51.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 449)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673334 sequences (100000060 bp) in 2.948 CPU sec, 3.165 real sec...
[M::process] read 673176 sequences (100000162 bp) in 1.930 CPU sec, 1.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285378, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.95, 132.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673404 reads in 240.407 CPU sec, 7.675 real sec
[M::mem_process_seqs] Processed 673384 reads in 133.312 CPU sec, 4.257 real sec
sorting block 45, #reads = 1077353 mem = 536882570 peak = 1004433408
sorting block 46, #reads = 1078948 mem = 536879059 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284103, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.51, 138.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673178 sequences (100000266 bp) in 3.714 CPU sec, 3.960 real sec...
[M::process] read 673172 sequences (100000016 bp) in 2.190 CPU sec, 2.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283961, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.55, 139.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673334 reads in 252.671 CPU sec, 8.048 real sec
[M::mem_process_seqs] Processed 673176 reads in 138.674 CPU sec, 4.397 real sec
sorting block 47, #reads = 1079005 mem = 536880607 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284280, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.93, 141.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 56, 985)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2941)
[M::mem_pestat] mean and std.dev: (498.40, 830.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3919)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673116 sequences (100000281 bp) in 3.112 CPU sec, 3.332 real sec...
[M::process] read 673032 sequences (100000142 bp) in 2.140 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284237, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.31, 141.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673178 reads in 253.407 CPU sec, 8.083 real sec
[M::mem_process_seqs] Processed 673172 reads in 136.953 CPU sec, 4.364 real sec
sorting block 48, #reads = 1080142 mem = 536878639 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284378, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.62, 146.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 25, 703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2097)
[M::mem_pestat] mean and std.dev: (223.25, 324.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2794)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672962 sequences (100000220 bp) in 3.582 CPU sec, 3.824 real sec...
[M::process] read 673612 sequences (100000151 bp) in 2.100 CPU sec, 2.094 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283746, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (276.45, 147.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673116 reads in 242.031 CPU sec, 7.697 real sec
[M::mem_process_seqs] Processed 673032 reads in 132.074 CPU sec, 4.203 real sec
sorting block 49, #reads = 1080008 mem = 536879472 peak = 1004433408
[M::process] read 673602 sequences (100000003 bp) in 2.478 CPU sec, 2.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284192, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (278.82, 150.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673670 sequences (100000073 bp) in 2.039 CPU sec, 2.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285230, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.50, 137.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285567, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.01, 134.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 56, 864)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2582)
[M::mem_pestat] mean and std.dev: (556.07, 792.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3726)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672962 reads in 322.141 CPU sec, 10.306 real sec
[M::mem_process_seqs] Processed 673612 reads in 217.893 CPU sec, 6.978 real sec
sorting block 50, #reads = 1079298 mem = 536879672 peak = 1004433408
[M::mem_process_seqs] Processed 673602 reads in 226.160 CPU sec, 7.262 real sec
sorting block 51, #reads = 1079184 mem = 536877604 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285992, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.86, 130.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 345, 1876)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5454)
[M::mem_pestat] mean and std.dev: (951.00, 1300.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7243)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673670 reads in 115.997 CPU sec, 3.840 real sec
[M::process] read 673568 sequences (100000203 bp) in 4.324 CPU sec, 4.721 real sec...
sorting block 52, #reads = 1079565 mem = 536879874 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285801, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.84, 132.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673568 reads in 114.847 CPU sec, 3.750 real sec
[M::process] read 673440 sequences (100000244 bp) in 3.922 CPU sec, 4.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285648, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.19, 135.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1080542 mem = 536877273 peak = 1004433408
[M::mem_process_seqs] Processed 673440 reads in 117.176 CPU sec, 3.801 real sec
[M::process] read 673486 sequences (100000080 bp) in 3.968 CPU sec, 4.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285506, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.48, 137.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 78, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077)
[M::mem_pestat] mean and std.dev: (239.11, 350.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1642)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673428 sequences (100000189 bp) in 3.286 CPU sec, 3.484 real sec...
[M::mem_process_seqs] Processed 673486 reads in 112.436 CPU sec, 3.656 real sec
sorting block 54, #reads = 1075042 mem = 536875969 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285938, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (266.94, 137.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 343, 595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1743)
[M::mem_pestat] mean and std.dev: (410.70, 412.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2317)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673428 reads in 115.520 CPU sec, 3.718 real sec
[M::process] read 673408 sequences (100000052 bp) in 4.536 CPU sec, 4.959 real sec...
[M::process] read 673456 sequences (100000292 bp) in 3.019 CPU sec, 3.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285775, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.57, 136.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673402 sequences (100000197 bp) in 2.010 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285520, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.51, 132.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673408 reads in 213.665 CPU sec, 6.727 real sec
[M::mem_process_seqs] Processed 673456 reads in 120.139 CPU sec, 3.797 real sec
sorting block 55, #reads = 1074708 mem = 536881752 peak = 1004433408
[M::process] read 673368 sequences (100000115 bp) in 2.896 CPU sec, 3.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284654, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.19, 137.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 85, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (91.38, 81.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 56, #reads = 1078473 mem = 536877276 peak = 1004433408
[M::process] read 673204 sequences (100000029 bp) in 2.998 CPU sec, 3.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284572, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (265.78, 138.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673208 sequences (100000078 bp) in 1.787 CPU sec, 1.794 real sec...
[M::mem_process_seqs] Processed 673402 reads in 228.735 CPU sec, 7.314 real sec
[M::mem_process_seqs] Processed 673368 reads in 125.881 CPU sec, 3.999 real sec
sorting block 57, #reads = 1079138 mem = 536876908 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284177, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (268.92, 141.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673118 sequences (100000133 bp) in 3.261 CPU sec, 3.460 real sec...
[M::process] read 673202 sequences (100000095 bp) in 2.020 CPU sec, 2.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284367, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.08, 140.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 2, 29)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 83)
[M::mem_pestat] mean and std.dev: (10.56, 12.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 110)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673204 reads in 233.199 CPU sec, 7.430 real sec
[M::mem_process_seqs] Processed 673208 reads in 128.202 CPU sec, 4.078 real sec
sorting block 58, #reads = 1079084 mem = 536879297 peak = 1004433408
[M::process] read 673226 sequences (100000131 bp) in 2.237 CPU sec, 2.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284080, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (272.94, 145.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673532 sequences (100000137 bp) in 1.490 CPU sec, 1.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284246, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (273.73, 146.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 81, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085)
[M::mem_pestat] mean and std.dev: (165.21, 185.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1443)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284592, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (271.61, 144.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673118 reads in 332.334 CPU sec, 10.664 real sec
[M::mem_process_seqs] Processed 673202 reads in 227.742 CPU sec, 7.331 real sec
sorting block 59, #reads = 1077929 mem = 536880318 peak = 1004433408
[M::mem_process_seqs] Processed 673226 reads in 237.585 CPU sec, 7.672 real sec
sorting block 60, #reads = 1077546 mem = 536879517 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284760, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.45, 136.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 87, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226)
[M::mem_pestat] mean and std.dev: (72.58, 43.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 287)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673532 reads in 123.006 CPU sec, 4.042 real sec
[M::process] read 673328 sequences (100000095 bp) in 3.947 CPU sec, 4.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284689, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.68, 136.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 61, #reads = 1077439 mem = 536878062 peak = 1004433408
[M::process] read 673466 sequences (100000095 bp) in 3.321 CPU sec, 3.601 real sec...
[M::mem_process_seqs] Processed 673328 reads in 114.302 CPU sec, 3.729 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284837, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.61, 134.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673466 reads in 115.126 CPU sec, 3.726 real sec
[M::process] read 673454 sequences (100000184 bp) in 3.890 CPU sec, 4.211 real sec...
sorting block 62, #reads = 1079452 mem = 536880055 peak = 1004433408
[M::process] read 673428 sequences (100000162 bp) in 2.856 CPU sec, 3.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285101, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.27, 132.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673266 sequences (100000237 bp) in 1.830 CPU sec, 1.828 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285344, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.67, 135.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673260 sequences (100000025 bp) in 1.910 CPU sec, 1.911 real sec...
[M::mem_process_seqs] Processed 673454 reads in 216.456 CPU sec, 6.811 real sec
[M::mem_process_seqs] Processed 673428 reads in 121.823 CPU sec, 3.827 real sec
sorting block 63, #reads = 1079712 mem = 536880656 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284408, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.65, 138.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673410 sequences (100000226 bp) in 3.356 CPU sec, 3.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285043, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.16, 138.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673266 reads in 208.060 CPU sec, 6.617 real sec
[M::process] read 673458 sequences (100000082 bp) in 2.224 CPU sec, 2.231 real sec...
[M::mem_process_seqs] Processed 673260 reads in 120.395 CPU sec, 3.796 real sec
sorting block 64, #reads = 1077502 mem = 536881074 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284281, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.55, 135.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 98, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (106.89, 85.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 643)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673338 sequences (100000043 bp) in 2.443 CPU sec, 2.581 real sec...
[M::process] read 673292 sequences (100000298 bp) in 2.000 CPU sec, 1.997 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285135, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.91, 135.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 52, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (133.62, 174.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673410 reads in 210.535 CPU sec, 6.710 real sec
sorting block 65, #reads = 1074728 mem = 536878670 peak = 1004433408
[M::mem_process_seqs] Processed 673458 reads in 121.649 CPU sec, 3.869 real sec
sorting block 66, #reads = 1077838 mem = 536880897 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283955, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.55, 137.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673292 sequences (100000083 bp) in 3.149 CPU sec, 3.412 real sec...
[M::process] read 673182 sequences (100000061 bp) in 1.970 CPU sec, 1.968 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284509, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.44, 139.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673338 reads in 224.649 CPU sec, 7.159 real sec
[M::mem_process_seqs] Processed 673292 reads in 123.978 CPU sec, 3.927 real sec
sorting block 67, #reads = 1079485 mem = 536879251 peak = 1004433408
[M::process] read 673192 sequences (100000165 bp) in 2.439 CPU sec, 2.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284078, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.71, 141.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673240 sequences (100000218 bp) in 1.980 CPU sec, 1.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284340, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.49, 146.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284105, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.10, 142.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673292 reads in 327.557 CPU sec, 10.481 real sec
[M::mem_process_seqs] Processed 673192 reads in 225.622 CPU sec, 7.235 real sec
[M::mem_process_seqs] Processed 673182 reads in 233.977 CPU sec, 7.524 real sec
sorting block 68, #reads = 1079583 mem = 536878556 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285195, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (271.28, 145.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673240 reads in 112.138 CPU sec, 3.720 real sec
sorting block 69, #reads = 1079250 mem = 536882735 peak = 1004433408
[M::process] read 673192 sequences (100000117 bp) in 3.644 CPU sec, 4.050 real sec...
[M::process] read 673420 sequences (100000009 bp) in 2.350 CPU sec, 2.547 real sec...
sorting block 70, #reads = 1076677 mem = 536876616 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285165, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (272.73, 145.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673234 sequences (100000141 bp) in 1.490 CPU sec, 1.638 real sec...
[M::process] read 673316 sequences (100000267 bp) in 1.430 CPU sec, 1.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284804, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.17, 141.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 94, 3796)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11320)
[M::mem_pestat] mean and std.dev: (1076.19, 1590.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15082)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673192 reads in 214.296 CPU sec, 6.811 real sec
[M::mem_process_seqs] Processed 673420 reads in 122.573 CPU sec, 3.894 real sec
sorting block 71, #reads = 1078830 mem = 536882921 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284623, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.65, 142.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673386 sequences (100000135 bp) in 3.228 CPU sec, 3.404 real sec...
[M::process] read 673288 sequences (100000042 bp) in 1.650 CPU sec, 1.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285009, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.59, 137.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 90, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (165.09, 139.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1311)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673234 reads in 215.112 CPU sec, 6.859 real sec
[M::mem_process_seqs] Processed 673316 reads in 116.649 CPU sec, 3.711 real sec
sorting block 72, #reads = 1079332 mem = 536878551 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285448, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.70, 135.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673212 sequences (100000203 bp) in 2.445 CPU sec, 2.647 real sec...
[M::process] read 673226 sequences (100000269 bp) in 2.040 CPU sec, 2.041 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285105, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.42, 135.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673386 reads in 208.048 CPU sec, 6.631 real sec
[M::mem_process_seqs] Processed 673288 reads in 115.412 CPU sec, 3.667 real sec
sorting block 73, #reads = 1079974 mem = 536878249 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284590, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.13, 140.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673298 sequences (100000040 bp) in 3.347 CPU sec, 3.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284638, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.33, 138.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (70, 86, 691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1933)
[M::mem_pestat] mean and std.dev: (338.31, 362.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2554)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673410 sequences (100000049 bp) in 2.070 CPU sec, 2.074 real sec...
[M::mem_process_seqs] Processed 673212 reads in 206.259 CPU sec, 6.551 real sec
[M::mem_process_seqs] Processed 673226 reads in 114.684 CPU sec, 3.626 real sec
sorting block 74, #reads = 1079225 mem = 536878721 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285069, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.76, 134.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 75, #reads = 1076979 mem = 536878529 peak = 1004433408
[M::process] read 673350 sequences (100000189 bp) in 2.835 CPU sec, 3.084 real sec...
[M::process] read 673234 sequences (100000196 bp) in 1.820 CPU sec, 1.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284822, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.22, 136.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 16, 829)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2473)
[M::mem_pestat] mean and std.dev: (154.83, 302.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3295)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673298 reads in 214.263 CPU sec, 6.831 real sec
[M::mem_process_seqs] Processed 673410 reads in 119.247 CPU sec, 3.786 real sec
sorting block 76, #reads = 1080249 mem = 536878868 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285122, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.11, 139.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 118, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (149.89, 145.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673260 sequences (100000255 bp) in 2.834 CPU sec, 3.009 real sec...
[M::process] read 673084 sequences (100000091 bp) in 1.580 CPU sec, 1.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284148, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.26, 140.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673350 reads in 214.019 CPU sec, 6.828 real sec
[M::mem_process_seqs] Processed 673234 reads in 117.758 CPU sec, 3.756 real sec
sorting block 77, #reads = 1079766 mem = 536879877 peak = 1004433408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284054, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.70, 140.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 263, 764)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2146)
[M::mem_pestat] mean and std.dev: (380.91, 398.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2837)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673188 sequences (100000151 bp) in 2.957 CPU sec, 3.177 real sec...
[M::process] read 673022 sequences (100000141 bp) in 1.580 CPU sec, 1.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284297, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.85, 144.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 94, 691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1987)
[M::mem_pestat] mean and std.dev: (201.50, 259.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2635)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673260 reads in 216.204 CPU sec, 6.898 real sec
[M::mem_process_seqs] Processed 673084 reads in 119.224 CPU sec, 3.799 real sec
sorting block 78, #reads = 1079382 mem = 536879423 peak = 1004433408
[M::process] read 376352 sequences (55915801 bp) in 2.399 CPU sec, 2.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284948, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.42, 146.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284217, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (276.38, 149.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 158973, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (279.47, 152.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673188 reads in 264.640 CPU sec, 8.525 real sec
[M::mem_process_seqs] Processed 673022 reads in 168.915 CPU sec, 5.466 real sec
sorting block 79, #reads = 1079608 mem = 536878972 peak = 1004433408
[M::mem_process_seqs] Processed 376352 reads in 173.614 CPU sec, 5.630 real sec
sorting block 80, #reads = 1079529 mem = 536879656 peak = 1073541120
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 87226428
overall: 69568958464 mem 17912.519 user 779.318 sys 743.452 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L003\tPU:L003\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.T18-D0003255_R1.fastq.gz 0002.T18-D0003255_R2.fastq.gz
[main] Real time: 743.482 sec; CPU: 18691.841 sec
sorting block 81, #reads = 438001 mem = 217881940 peak = 1073541120
uniq header count 1
merging 82 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 3444577094
output reads: 87858995
bam_mem_sort: 82 calls 65.840 user 2.071 sys 68.006 real
bam_write: 82 calls 30.279 user 1.884 sys 32.183 real
execute: 1 calls 32.184 user 1.916 sys 780.575 real
merge_files: 1 calls 32.152 user 1.894 sys 36.658 real
parse_chunk: 1303 calls 64.146 user 15.490 sys 80.932 real
read_chunk: 3507 calls 38.571 user 28.193 sys 527.048 real
sort_block: 1 calls 25.628 user 27.442 sys 509.302 real
write_chunk: 2202 calls 31.612 user 1.594 sys 35.552 real
overall: 2285191168 mem 1077.417 user 70.557 sys 780.596 real
ls: cannot access '*.cram': No such file or directory