This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1850.765 CPU sec, 64.117 real sec
[M::process] read 677092 sequences (100000173 bp) in 1.060 CPU sec, 1.063 real sec...
[M::process] read 676712 sequences (100000228 bp) in 1.780 CPU sec, 1.842 real sec...
[M::process] read 676634 sequences (100000183 bp) in 1.647 CPU sec, 1.705 real sec...
[M::process] read 676826 sequences (100000140 bp) in 1.578 CPU sec, 1.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288098, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.89, 110.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 10, 175)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (71.50, 124.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 697)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676712 reads in 101.653 CPU sec, 3.340 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288745, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.27, 107.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 133, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362)
[M::mem_pestat] mean and std.dev: (193.56, 218.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1801)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676946 sequences (100000145 bp) in 1.568 CPU sec, 1.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288199, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.56, 112.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 61, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 148)
[M::mem_pestat] mean and std.dev: (71.57, 12.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 177)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677092 reads in 306.458 CPU sec, 9.970 real sec
[M::mem_process_seqs] Processed 676634 reads in 118.967 CPU sec, 3.884 real sec
sorting block 0, #reads = 1081921 mem = 536879051 peak = 604184576
[M::process] read 676832 sequences (100000224 bp) in 1.767 CPU sec, 1.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288050, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.95, 113.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676614 sequences (100000030 bp) in 1.541 CPU sec, 1.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287766, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.19, 110.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288036, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.82, 109.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676826 reads in 292.444 CPU sec, 9.568 real sec
sorting block 1, #reads = 1081801 mem = 536878610 peak = 959938560
[M::mem_process_seqs] Processed 676832 reads in 205.709 CPU sec, 6.796 real sec
[M::mem_process_seqs] Processed 676946 reads in 214.693 CPU sec, 7.128 real sec
[M::process] read 676726 sequences (100000150 bp) in 1.753 CPU sec, 1.969 real sec...
sorting block 2, #reads = 1081972 mem = 536880818 peak = 962318336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288011, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.56, 112.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 114, 628)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1854)
[M::mem_pestat] mean and std.dev: (105.50, 179.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2467)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676740 sequences (100000186 bp) in 1.718 CPU sec, 1.960 real sec...
[M::process] read 676676 sequences (100000165 bp) in 1.618 CPU sec, 1.640 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288045, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (232.19, 113.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669)
[M::mem_pestat] mean and std.dev: (125.85, 198.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2216)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676614 reads in 204.472 CPU sec, 6.676 real sec
[M::mem_process_seqs] Processed 676726 reads in 115.723 CPU sec, 3.694 real sec
sorting block 3, #reads = 1081936 mem = 536880809 peak = 975446016
[M::process] read 676332 sequences (100000139 bp) in 1.885 CPU sec, 2.000 real sec...
sorting block 4, #reads = 1080990 mem = 536880715 peak = 980709376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288011, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.13, 115.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676116 sequences (100000083 bp) in 1.490 CPU sec, 1.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288133, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.85, 114.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 4, 695)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2077)
[M::mem_pestat] mean and std.dev: (78.64, 196.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2768)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287810, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.37, 117.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676740 reads in 295.846 CPU sec, 9.555 real sec
[M::mem_process_seqs] Processed 676676 reads in 206.944 CPU sec, 6.669 real sec
[M::mem_process_seqs] Processed 676332 reads in 215.880 CPU sec, 6.998 real sec
sorting block 5, #reads = 1082106 mem = 536879829 peak = 980709376
[M::process] read 676582 sequences (100000248 bp) in 2.019 CPU sec, 2.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287646, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.76, 120.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676810 sequences (100000074 bp) in 1.921 CPU sec, 1.988 real sec...
[M::process] read 676692 sequences (100000153 bp) in 1.576 CPU sec, 1.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288193, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.65, 114.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676116 reads in 202.144 CPU sec, 6.454 real sec
sorting block 6, #reads = 1081710 mem = 536876644 peak = 992120832
[M::mem_process_seqs] Processed 676582 reads in 112.582 CPU sec, 3.573 real sec
sorting block 7, #reads = 1081844 mem = 536880077 peak = 997662720
[M::process] read 676546 sequences (100000269 bp) in 1.821 CPU sec, 1.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288429, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.95, 111.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676712 sequences (100000139 bp) in 1.602 CPU sec, 1.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287999, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.63, 115.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288442, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.32, 113.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676810 reads in 286.532 CPU sec, 9.239 real sec
[M::mem_process_seqs] Processed 676546 reads in 201.333 CPU sec, 6.459 real sec
[M::mem_process_seqs] Processed 676692 reads in 210.670 CPU sec, 6.779 real sec
sorting block 8, #reads = 1082053 mem = 536878911 peak = 997662720
[M::process] read 676804 sequences (100000274 bp) in 2.045 CPU sec, 2.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287943, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.46, 115.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 28, 106)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298)
[M::mem_pestat] mean and std.dev: (42.62, 38.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 394)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 9, #reads = 1082280 mem = 536879450 peak = 1008017408
[M::process] read 676850 sequences (100000043 bp) in 1.762 CPU sec, 2.018 real sec...
[M::process] read 676882 sequences (100000193 bp) in 1.658 CPU sec, 1.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288053, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.13, 112.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 100, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (166.33, 200.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676712 reads in 201.479 CPU sec, 6.583 real sec
[M::mem_process_seqs] Processed 676804 reads in 114.661 CPU sec, 3.643 real sec
sorting block 10, #reads = 1082161 mem = 536880680 peak = 1008017408
[M::process] read 676628 sequences (100000191 bp) in 2.007 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287705, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.51, 113.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676618 sequences (100000018 bp) in 1.624 CPU sec, 1.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288122, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.86, 114.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676850 reads in 201.481 CPU sec, 6.507 real sec
[M::mem_process_seqs] Processed 676882 reads in 113.142 CPU sec, 3.652 real sec
sorting block 11, #reads = 1082407 mem = 536878202 peak = 1008017408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288184, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.76, 114.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676450 sequences (100000251 bp) in 1.823 CPU sec, 2.305 real sec...
[M::process] read 676270 sequences (100000142 bp) in 1.652 CPU sec, 1.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287747, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.65, 115.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676628 reads in 200.392 CPU sec, 6.489 real sec
[M::mem_process_seqs] Processed 676618 reads in 113.911 CPU sec, 3.651 real sec
sorting block 12, #reads = 1082309 mem = 536880785 peak = 1008017408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288158, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.60, 118.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 51, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1785)
[M::mem_pestat] mean and std.dev: (166.00, 210.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2367)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675948 sequences (100000159 bp) in 2.033 CPU sec, 2.583 real sec...
[M::process] read 676680 sequences (100000227 bp) in 1.699 CPU sec, 1.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288089, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.68, 120.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676450 reads in 198.693 CPU sec, 6.420 real sec
[M::mem_process_seqs] Processed 676270 reads in 111.804 CPU sec, 3.564 real sec
sorting block 13, #reads = 1082198 mem = 536877410 peak = 1025970176
sorting block 14, #reads = 1082104 mem = 536879251 peak = 1029918720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288203, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (243.61, 123.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676648 sequences (100000239 bp) in 2.075 CPU sec, 2.313 real sec...
[M::process] read 676568 sequences (100000180 bp) in 1.670 CPU sec, 1.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288039, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.84, 111.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675948 reads in 195.373 CPU sec, 6.320 real sec
[M::mem_process_seqs] Processed 676680 reads in 109.619 CPU sec, 3.504 real sec
sorting block 15, #reads = 1081411 mem = 536879666 peak = 1029918720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288611, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.80, 113.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676342 sequences (100000050 bp) in 2.148 CPU sec, 2.558 real sec...
[M::process] read 676754 sequences (100000155 bp) in 1.634 CPU sec, 1.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288572, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (236.01, 115.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676648 reads in 192.700 CPU sec, 6.213 real sec
[M::mem_process_seqs] Processed 676568 reads in 108.858 CPU sec, 3.486 real sec
sorting block 16, #reads = 1081754 mem = 536877602 peak = 1029918720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288111, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.16, 117.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676678 sequences (100000298 bp) in 2.071 CPU sec, 2.485 real sec...
[M::process] read 676644 sequences (100000143 bp) in 1.678 CPU sec, 1.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288422, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.07, 114.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 198, 4120)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12134)
[M::mem_pestat] mean and std.dev: (1830.64, 1983.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16141)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676342 reads in 195.710 CPU sec, 6.314 real sec
[M::mem_process_seqs] Processed 676754 reads in 111.893 CPU sec, 3.567 real sec
sorting block 17, #reads = 1082045 mem = 536879458 peak = 1029918720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288294, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676548 sequences (100000278 bp) in 2.041 CPU sec, 2.414 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.32, 110.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676380 sequences (100000186 bp) in 1.771 CPU sec, 1.776 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287991, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.49, 114.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676678 reads in 199.054 CPU sec, 6.439 real sec
sorting block 18, #reads = 1082438 mem = 536876965 peak = 1037279232
[M::mem_process_seqs] Processed 676644 reads in 111.949 CPU sec, 3.593 real sec
sorting block 19, #reads = 1082063 mem = 536879907 peak = 1044848640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288450, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.46, 116.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 91, 611)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1781)
[M::mem_pestat] mean and std.dev: (316.50, 488.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2366)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676556 sequences (100000091 bp) in 1.998 CPU sec, 2.380 real sec...
[M::process] read 676186 sequences (100000040 bp) in 1.828 CPU sec, 1.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285692, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.55, 116.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 13, 13)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 27)
[M::mem_pestat] mean and std.dev: (8.64, 3.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 34)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676548 reads in 197.776 CPU sec, 6.414 real sec
[M::mem_process_seqs] Processed 676380 reads in 115.100 CPU sec, 3.681 real sec
sorting block 20, #reads = 1081984 mem = 536878174 peak = 1044848640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288394, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.97, 115.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676184 sequences (100000203 bp) in 2.139 CPU sec, 2.558 real sec...
[M::process] read 676400 sequences (100000197 bp) in 1.642 CPU sec, 1.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287685, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.82, 119.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 370, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174)
[M::mem_pestat] mean and std.dev: (308.00, 308.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1550)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676556 reads in 200.188 CPU sec, 6.457 real sec
[M::mem_process_seqs] Processed 676186 reads in 113.854 CPU sec, 3.631 real sec
sorting block 21, #reads = 1079786 mem = 536882501 peak = 1044848640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288128, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.49, 120.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676796 sequences (100000238 bp) in 1.871 CPU sec, 2.453 real sec...
[M::process] read 676560 sequences (100000167 bp) in 1.696 CPU sec, 1.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287938, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.70, 117.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 184, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1935)
[M::mem_pestat] mean and std.dev: (304.40, 279.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2575)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676184 reads in 196.737 CPU sec, 6.381 real sec
[M::mem_process_seqs] Processed 676400 reads in 110.378 CPU sec, 3.519 real sec
sorting block 22, #reads = 1081356 mem = 536878817 peak = 1056497664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288389, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.05, 111.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 93, 1349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4015)
[M::mem_pestat] mean and std.dev: (392.31, 542.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5348)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676402 sequences (100000201 bp) in 2.151 CPU sec, 2.455 real sec...
[M::process] read 676190 sequences (100000120 bp) in 1.665 CPU sec, 1.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288183, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.62, 113.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676796 reads in 196.707 CPU sec, 6.337 real sec
sorting block 23, #reads = 1081253 mem = 536881616 peak = 1059737600
[M::mem_process_seqs] Processed 676560 reads in 111.006 CPU sec, 3.533 real sec
sorting block 24, #reads = 1082260 mem = 536878709 peak = 1069043712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288050, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.98, 115.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676894 sequences (100000102 bp) in 2.099 CPU sec, 2.395 real sec...
[M::process] read 676522 sequences (100000253 bp) in 1.545 CPU sec, 1.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288161, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 181, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.16, 118.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 621, 1288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3796)
[M::mem_pestat] mean and std.dev: (791.50, 840.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5050)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676402 reads in 199.944 CPU sec, 6.484 real sec
[M::mem_process_seqs] Processed 676190 reads in 113.334 CPU sec, 3.704 real sec
sorting block 25, #reads = 1082308 mem = 536878500 peak = 1069043712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288527, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.03, 109.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676586 sequences (100000175 bp) in 2.046 CPU sec, 2.355 real sec...
[M::process] read 676474 sequences (100000101 bp) in 1.689 CPU sec, 1.692 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288230, 22, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.18, 113.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 29, 125)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349)
[M::mem_pestat] mean and std.dev: (35.67, 36.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 461)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676894 reads in 200.095 CPU sec, 6.485 real sec
sorting block 26, #reads = 1082252 mem = 536881091 peak = 1069043712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288412, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.84, 115.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676522 sequences (100000274 bp) in 1.740 CPU sec, 1.871 real sec...
[M::mem_process_seqs] Processed 676586 reads in 105.670 CPU sec, 3.463 real sec
[M::mem_process_seqs] Processed 676522 reads in 252.444 CPU sec, 8.195 real sec
sorting block 27, #reads = 1082640 mem = 536878722 peak = 1069043712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287593, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.14, 116.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676424 sequences (100000241 bp) in 2.010 CPU sec, 2.219 real sec...
[M::process] read 676336 sequences (100000091 bp) in 1.710 CPU sec, 1.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287598, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.51, 116.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676474 reads in 210.938 CPU sec, 6.768 real sec
[M::mem_process_seqs] Processed 676522 reads in 112.848 CPU sec, 3.612 real sec
sorting block 28, #reads = 1082204 mem = 536879774 peak = 1069043712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287465, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.67, 118.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676116 sequences (100000250 bp) in 2.511 CPU sec, 2.920 real sec...
[M::process] read 677028 sequences (100000199 bp) in 1.716 CPU sec, 1.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287689, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.22, 119.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676424 reads in 204.584 CPU sec, 6.609 real sec
[M::mem_process_seqs] Processed 676336 reads in 114.434 CPU sec, 3.682 real sec
sorting block 29, #reads = 1082144 mem = 536880888 peak = 1070776320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288083, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677094 sequences (100000032 bp) in 2.069 CPU sec, 2.459 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.02, 120.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676922 sequences (100000203 bp) in 1.690 CPU sec, 1.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288173, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.69, 113.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676116 reads in 201.548 CPU sec, 6.531 real sec
[M::mem_process_seqs] Processed 677028 reads in 114.954 CPU sec, 3.670 real sec
sorting block 30, #reads = 1081855 mem = 536879828 peak = 1098358784
sorting block 31, #reads = 1081936 mem = 536876430 peak = 1104502784
[M::process] read 676868 sequences (100000050 bp) in 2.079 CPU sec, 2.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287654, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.36, 110.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677072 sequences (100000004 bp) in 1.809 CPU sec, 1.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287625, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.82, 112.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677094 reads in 208.579 CPU sec, 6.729 real sec
[M::mem_process_seqs] Processed 676922 reads in 117.683 CPU sec, 3.758 real sec
sorting block 32, #reads = 1081839 mem = 536879541 peak = 1104502784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287688, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::process] read 677258 sequences (100000052 bp) in 2.120 CPU sec, 2.520 real sec...
[M::mem_pestat] mean and std.dev: (231.93, 113.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 998, 9428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28138)
[M::mem_pestat] mean and std.dev: (3651.00, 4334.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 37493)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676938 sequences (100000110 bp) in 1.732 CPU sec, 1.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287887, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.81, 108.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676868 reads in 212.617 CPU sec, 6.874 real sec
[M::mem_process_seqs] Processed 677072 reads in 120.266 CPU sec, 3.838 real sec
sorting block 33, #reads = 1081746 mem = 536878967 peak = 1104502784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288025, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.60, 107.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677002 sequences (100000183 bp) in 2.227 CPU sec, 2.688 real sec...
[M::process] read 676954 sequences (100000242 bp) in 1.718 CPU sec, 1.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287750, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.62, 108.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677258 reads in 211.762 CPU sec, 6.871 real sec
[M::mem_process_seqs] Processed 676938 reads in 119.353 CPU sec, 3.827 real sec
sorting block 34, #reads = 1082634 mem = 536878378 peak = 1104502784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288138, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.86, 109.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676884 sequences (100000245 bp) in 2.394 CPU sec, 2.523 real sec...
[M::process] read 676614 sequences (100000198 bp) in 1.690 CPU sec, 1.701 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287799, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.38, 107.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 90, 100)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280)
[M::mem_pestat] mean and std.dev: (50.44, 41.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 370)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677002 reads in 204.485 CPU sec, 6.601 real sec
sorting block 35, #reads = 1082620 mem = 536881338 peak = 1104502784
[M::mem_process_seqs] Processed 676954 reads in 112.298 CPU sec, 3.607 real sec
sorting block 36, #reads = 1082551 mem = 536878596 peak = 1123733504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287803, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.34, 112.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676770 sequences (100000151 bp) in 2.247 CPU sec, 2.506 real sec...
[M::process] read 676554 sequences (100000017 bp) in 1.757 CPU sec, 1.763 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287852, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.09, 114.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 97, 170)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486)
[M::mem_pestat] mean and std.dev: (56.12, 55.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 644)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676884 reads in 201.745 CPU sec, 6.545 real sec
[M::mem_process_seqs] Processed 676614 reads in 114.210 CPU sec, 3.665 real sec
sorting block 37, #reads = 1081781 mem = 536877846 peak = 1123733504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287963, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.45, 116.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677308 sequences (100000062 bp) in 2.317 CPU sec, 2.599 real sec...
[M::process] read 677048 sequences (100000011 bp) in 1.911 CPU sec, 1.921 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287847, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.18, 116.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676770 reads in 199.173 CPU sec, 6.399 real sec
[M::mem_process_seqs] Processed 676554 reads in 112.395 CPU sec, 3.581 real sec
sorting block 38, #reads = 1082518 mem = 536879980 peak = 1123733504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288336, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.68, 108.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676882 sequences (100000175 bp) in 2.156 CPU sec, 2.474 real sec...
[M::process] read 676800 sequences (100000246 bp) in 1.744 CPU sec, 1.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288060, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (229.20, 111.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 79, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2016)
[M::mem_pestat] mean and std.dev: (257.29, 463.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2682)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677308 reads in 192.368 CPU sec, 6.185 real sec
sorting block 39, #reads = 1081937 mem = 536878849 peak = 1123733504
[M::process] read 677156 sequences (100000115 bp) in 1.945 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287708, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.60, 113.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 266, 2468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7302)
[M::mem_pestat] mean and std.dev: (735.69, 969.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9719)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676882 reads in 107.213 CPU sec, 3.435 real sec
[M::mem_process_seqs] Processed 677048 reads in 249.411 CPU sec, 7.986 real sec
sorting block 40, #reads = 1082049 mem = 536881116 peak = 1123733504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287660, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.35, 116.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677122 sequences (100000295 bp) in 2.319 CPU sec, 2.643 real sec...
[M::process] read 677100 sequences (100000099 bp) in 1.718 CPU sec, 1.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288259, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.25, 107.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676800 reads in 207.201 CPU sec, 6.666 real sec
[M::mem_process_seqs] Processed 677156 reads in 111.816 CPU sec, 3.560 real sec
sorting block 41, #reads = 1082323 mem = 536882831 peak = 1123733504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288357, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.30, 107.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676744 sequences (100000191 bp) in 2.124 CPU sec, 2.392 real sec...
[M::process] read 676872 sequences (100000089 bp) in 1.767 CPU sec, 1.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287852, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.32, 109.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677122 reads in 198.930 CPU sec, 6.401 real sec
[M::mem_process_seqs] Processed 677100 reads in 113.131 CPU sec, 3.625 real sec
sorting block 42, #reads = 1082571 mem = 536877563 peak = 1123733504
sorting block 43, #reads = 1082682 mem = 536878105 peak = 1123733504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284032, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (225.89, 109.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676690 sequences (100000227 bp) in 2.483 CPU sec, 2.665 real sec...
[M::process] read 676712 sequences (100000091 bp) in 1.767 CPU sec, 1.784 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288047, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.25, 111.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676744 reads in 199.684 CPU sec, 6.412 real sec
[M::mem_process_seqs] Processed 676872 reads in 114.881 CPU sec, 3.694 real sec
sorting block 44, #reads = 1077808 mem = 536879799 peak = 1123733504
[M::process] read 676558 sequences (100000035 bp) in 2.038 CPU sec, 2.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287483, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.77, 113.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677130 sequences (100000208 bp) in 1.710 CPU sec, 1.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287921, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.21, 115.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676690 reads in 201.723 CPU sec, 6.511 real sec
[M::mem_process_seqs] Processed 676712 reads in 112.848 CPU sec, 3.632 real sec
sorting block 45, #reads = 1082275 mem = 536879585 peak = 1123733504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287436, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.68, 117.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676796 sequences (100000003 bp) in 2.433 CPU sec, 2.686 real sec...
[M::process] read 676652 sequences (100000271 bp) in 1.846 CPU sec, 1.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288453, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (229.34, 111.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676558 reads in 195.026 CPU sec, 6.278 real sec
[M::mem_process_seqs] Processed 677130 reads in 108.955 CPU sec, 3.454 real sec
sorting block 46, #reads = 1082413 mem = 536877747 peak = 1123733504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287937, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.72, 113.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676674 sequences (100000230 bp) in 2.258 CPU sec, 2.440 real sec...
[M::process] read 676856 sequences (100000068 bp) in 1.853 CPU sec, 1.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288112, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.48, 115.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 28, 36)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 80)
[M::mem_pestat] mean and std.dev: (18.30, 12.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 102)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676796 reads in 193.973 CPU sec, 6.214 real sec
sorting block 47, #reads = 1081859 mem = 536879206 peak = 1123733504
[M::mem_process_seqs] Processed 676652 reads in 110.718 CPU sec, 3.527 real sec
sorting block 48, #reads = 1081892 mem = 536877247 peak = 1126592512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288098, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.03, 116.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676970 sequences (100000106 bp) in 2.435 CPU sec, 2.783 real sec...
[M::process] read 676864 sequences (100000237 bp) in 1.775 CPU sec, 1.784 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288096, 13, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.94, 110.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 85, 182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (110.00, 61.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 554)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676674 reads in 195.748 CPU sec, 6.294 real sec
[M::mem_process_seqs] Processed 676856 reads in 109.549 CPU sec, 3.469 real sec
sorting block 49, #reads = 1082178 mem = 536881604 peak = 1126592512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288560, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.01, 107.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676864 sequences (100000188 bp) in 2.203 CPU sec, 2.782 real sec...
[M::process] read 676730 sequences (100000167 bp) in 1.802 CPU sec, 1.840 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288185, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.42, 110.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 9, 75)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223)
[M::mem_pestat] mean and std.dev: (24.82, 33.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 297)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676970 reads in 197.595 CPU sec, 6.410 real sec
[M::mem_process_seqs] Processed 676864 reads in 114.406 CPU sec, 3.656 real sec
sorting block 50, #reads = 1082467 mem = 536878530 peak = 1126592512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288043, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.37, 109.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676748 sequences (100000267 bp) in 2.476 CPU sec, 2.909 real sec...
[M::process] read 676732 sequences (100000051 bp) in 1.856 CPU sec, 1.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288215, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.73, 112.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676864 reads in 194.240 CPU sec, 6.258 real sec
[M::mem_process_seqs] Processed 676730 reads in 111.958 CPU sec, 3.561 real sec
sorting block 51, #reads = 1082165 mem = 536881790 peak = 1126592512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287251, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.12, 113.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676538 sequences (100000213 bp) in 2.137 CPU sec, 2.681 real sec...
[M::process] read 677034 sequences (100000194 bp) in 1.910 CPU sec, 1.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287421, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.40, 115.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676748 reads in 200.352 CPU sec, 6.498 real sec
sorting block 52, #reads = 1081934 mem = 536880134 peak = 1130385408
[M::mem_process_seqs] Processed 676732 reads in 113.345 CPU sec, 3.647 real sec
sorting block 53, #reads = 1081965 mem = 536878595 peak = 1130393600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287781, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.52, 116.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676968 sequences (100000077 bp) in 2.604 CPU sec, 3.009 real sec...
[M::process] read 676810 sequences (100000064 bp) in 1.809 CPU sec, 1.819 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287985, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (230.43, 114.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676538 reads in 193.458 CPU sec, 6.269 real sec
[M::mem_process_seqs] Processed 677034 reads in 110.539 CPU sec, 3.519 real sec
sorting block 54, #reads = 1081237 mem = 536878700 peak = 1130393600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287792, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.58, 115.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 441, 1172)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3104)
[M::mem_pestat] mean and std.dev: (701.95, 681.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4070)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676582 sequences (100000271 bp) in 2.391 CPU sec, 2.655 real sec...
[M::process] read 676518 sequences (100000134 bp) in 1.789 CPU sec, 1.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287698, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.35, 116.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 44, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1593)
[M::mem_pestat] mean and std.dev: (205.58, 335.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2123)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676968 reads in 201.024 CPU sec, 6.479 real sec
[M::mem_process_seqs] Processed 676810 reads in 114.500 CPU sec, 3.690 real sec
sorting block 55, #reads = 1082245 mem = 536877561 peak = 1130393600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288114, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.95, 117.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 75, 92)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 182)
[M::mem_pestat] mean and std.dev: (54.88, 33.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 227)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676844 sequences (100000299 bp) in 2.522 CPU sec, 2.873 real sec...
[M::process] read 676766 sequences (100000100 bp) in 1.857 CPU sec, 1.858 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287836, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.02, 114.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676582 reads in 198.765 CPU sec, 6.438 real sec
[M::mem_process_seqs] Processed 676518 reads in 111.603 CPU sec, 3.595 real sec
sorting block 56, #reads = 1082132 mem = 536881492 peak = 1130393600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288260, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.77, 108.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (85, 211, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (142.78, 73.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 593)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676868 sequences (100000050 bp) in 2.374 CPU sec, 2.864 real sec...
[M::process] read 676842 sequences (100000243 bp) in 2.016 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288016, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.43, 110.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676844 reads in 197.457 CPU sec, 6.402 real sec
sorting block 57, #reads = 1081849 mem = 536879604 peak = 1131552768
[M::mem_process_seqs] Processed 676766 reads in 112.305 CPU sec, 3.561 real sec
sorting block 58, #reads = 1082595 mem = 536878500 peak = 1131552768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287681, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.01, 112.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676654 sequences (100000105 bp) in 2.591 CPU sec, 2.919 real sec...
[M::process] read 676628 sequences (100000087 bp) in 1.852 CPU sec, 1.860 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287997, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.85, 114.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676868 reads in 201.222 CPU sec, 6.473 real sec
[M::mem_process_seqs] Processed 676842 reads in 113.746 CPU sec, 3.611 real sec
sorting block 59, #reads = 1082685 mem = 536880034 peak = 1131552768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287882, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.92, 114.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676412 sequences (100000115 bp) in 2.369 CPU sec, 2.812 real sec...
[M::process] read 445552 sequences (65874949 bp) in 1.285 CPU sec, 1.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287621, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.82, 116.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676654 reads in 204.397 CPU sec, 6.633 real sec
[M::mem_process_seqs] Processed 676628 reads in 115.078 CPU sec, 3.686 real sec
sorting block 60, #reads = 1082579 mem = 536879044 peak = 1131552768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287628, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.62, 117.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 189099, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.16, 119.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 9, 27)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 67)
[M::mem_pestat] mean and std.dev: (10.90, 8.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 87)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676412 reads in 166.453 CPU sec, 5.464 real sec
[M::mem_process_seqs] Processed 445552 reads in 78.021 CPU sec, 2.537 real sec
sorting block 61, #reads = 1082173 mem = 536879857 peak = 1131552768
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67439410
overall: 69648977920 mem 11962.610 user 307.777 sys 410.543 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L001\tPU:L001\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.T21-D0003376_R1.fastq.gz 0004.T21-D0003376_R2.fastq.gz
[main] Real time: 410.582 sec; CPU: 12270.391 sec
sorting block 62, #reads = 886937 mem = 440068647 peak = 1131552768
uniq header count 1
merging 63 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2649302868
output reads: 67970120
bam_mem_sort: 63 calls 43.209 user 1.387 sys 46.351 real
bam_write: 63 calls 41.393 user 3.178 sys 90.361 real
execute: 1 calls 18.232 user 0.614 sys 431.712 real
merge_files: 1 calls 18.196 user 0.597 sys 20.329 real
parse_chunk: 1006 calls 61.387 user 14.549 sys 98.638 real
read_chunk: 2708 calls 28.472 user 13.425 sys 343.540 real
sort_block: 1 calls 23.417 user 13.128 sys 339.390 real
write_chunk: 1700 calls 17.718 user 0.440 sys 19.412 real
overall: 2354573312 mem 820.747 user 43.881 sys 431.734 real
ls: cannot access '*.cram': No such file or directory