Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b4/413f83ebaf1f1a900418e254902c5e/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0f/3075612d2ed405a2b0aedbd22c6861/T21-D0003376/0003.T21-D0003376_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0f/3075612d2ed405a2b0aedbd22c6861/T21-D0003376/0003.T21-D0003376_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b4/413f83ebaf1f1a900418e254902c5e/.command.run
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2072.281 CPU sec, 72.267 real sec
[M::process] read 676794 sequences (100000024 bp) in 0.986 CPU sec, 0.987 real sec...
[M::process] read 676514 sequences (100000098 bp) in 1.497 CPU sec, 1.546 real sec...
[M::process] read 676376 sequences (100000046 bp) in 1.500 CPU sec, 1.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288408, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.90, 109.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 224, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1765)
[M::mem_pestat] mean and std.dev: (180.00, 190.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2329)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676236 sequences (100000155 bp) in 1.434 CPU sec, 1.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288622, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (235.15, 114.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 100, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1970)
[M::mem_pestat] mean and std.dev: (148.25, 205.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2613)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288145, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.13, 112.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 91, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1656)
[M::mem_pestat] mean and std.dev: (292.57, 262.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2182)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676794 reads in 288.842 CPU sec, 9.376 real sec
[M::mem_process_seqs] Processed 676376 reads in 184.457 CPU sec, 6.093 real sec
[M::mem_process_seqs] Processed 676514 reads in 192.686 CPU sec, 6.397 real sec
sorting block 0, #reads = 1081992 mem = 536879851 peak = 623513600
[M::process] read 675944 sequences (100000003 bp) in 1.829 CPU sec, 2.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287615, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.64, 119.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 28, 51)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 115)
[M::mem_pestat] mean and std.dev: (24.45, 17.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 147)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675948 sequences (100000078 bp) in 1.461 CPU sec, 1.517 real sec...
[M::process] read 676144 sequences (100000125 bp) in 1.384 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288223, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.28, 122.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676236 reads in 183.927 CPU sec, 5.909 real sec
sorting block 1, #reads = 1081715 mem = 536877221 peak = 944173056
[M::mem_process_seqs] Processed 675944 reads in 101.608 CPU sec, 3.239 real sec
sorting block 2, #reads = 1081822 mem = 536879111 peak = 944173056
[M::process] read 676554 sequences (100000237 bp) in 1.953 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287342, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.27, 123.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 419, 8118)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24274)
[M::mem_pestat] mean and std.dev: (2154.17, 3447.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 32352)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676776 sequences (100000109 bp) in 1.391 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287847, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.09, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288125, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.15, 112.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675948 reads in 267.128 CPU sec, 8.639 real sec
[M::mem_process_seqs] Processed 676144 reads in 185.912 CPU sec, 6.070 real sec
[M::mem_process_seqs] Processed 676554 reads in 193.669 CPU sec, 6.352 real sec
sorting block 3, #reads = 1082259 mem = 536881035 peak = 944173056
[M::process] read 676618 sequences (100000075 bp) in 1.675 CPU sec, 1.856 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288443, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.94, 111.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 45, 45)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 125)
[M::mem_pestat] mean and std.dev: (22.62, 18.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 165)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 4, #reads = 1082390 mem = 536878291 peak = 944173056
[M::process] read 676450 sequences (100000221 bp) in 1.849 CPU sec, 2.015 real sec...
[M::process] read 676430 sequences (100000227 bp) in 1.443 CPU sec, 1.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288074, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.59, 112.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 107, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (199.00, 202.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1362)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676776 reads in 183.147 CPU sec, 5.886 real sec
[M::mem_process_seqs] Processed 676618 reads in 102.711 CPU sec, 3.279 real sec
sorting block 5, #reads = 1082876 mem = 536878084 peak = 944173056
[M::process] read 676230 sequences (100000171 bp) in 1.923 CPU sec, 2.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288355, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.90, 114.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676718 sequences (100000055 bp) in 1.405 CPU sec, 1.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288051, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.95, 118.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287620, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (236.19, 116.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676450 reads in 262.578 CPU sec, 8.492 real sec
[M::mem_process_seqs] Processed 676430 reads in 184.328 CPU sec, 5.956 real sec
[M::mem_process_seqs] Processed 676230 reads in 192.737 CPU sec, 6.264 real sec
sorting block 6, #reads = 1082465 mem = 536878960 peak = 944173056
[M::process] read 676558 sequences (100000151 bp) in 2.057 CPU sec, 2.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288275, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.14, 111.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1081748 mem = 536878060 peak = 944173056
[M::process] read 676356 sequences (100000100 bp) in 1.955 CPU sec, 2.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287802, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676186 sequences (100000095 bp) in 1.435 CPU sec, 1.442 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.95, 114.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676718 reads in 184.880 CPU sec, 5.943 real sec
[M::mem_process_seqs] Processed 676558 reads in 104.342 CPU sec, 3.321 real sec
sorting block 8, #reads = 1082138 mem = 536879768 peak = 944173056
[M::process] read 675942 sequences (100000029 bp) in 1.982 CPU sec, 2.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287767, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.65, 116.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 211, 1093)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3217)
[M::mem_pestat] mean and std.dev: (578.64, 763.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4279)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675912 sequences (100000256 bp) in 1.451 CPU sec, 1.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286951, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (240.69, 121.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287339, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (244.45, 123.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676356 reads in 272.482 CPU sec, 8.792 real sec
[M::mem_process_seqs] Processed 676186 reads in 193.083 CPU sec, 6.250 real sec
[M::mem_process_seqs] Processed 675942 reads in 200.752 CPU sec, 6.518 real sec
sorting block 9, #reads = 1082025 mem = 536876625 peak = 944173056
[M::process] read 676222 sequences (100000068 bp) in 1.912 CPU sec, 2.086 real sec...
sorting block 10, #reads = 1081540 mem = 536876806 peak = 944173056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287245, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (244.07, 124.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676982 sequences (100000244 bp) in 2.009 CPU sec, 2.145 real sec...
[M::process] read 676736 sequences (100000057 bp) in 1.439 CPU sec, 1.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287550, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.89, 120.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675912 reads in 194.831 CPU sec, 6.265 real sec
[M::mem_process_seqs] Processed 676222 reads in 107.008 CPU sec, 3.416 real sec
sorting block 11, #reads = 1081684 mem = 536877271 peak = 944173056
[M::process] read 676638 sequences (100000122 bp) in 1.949 CPU sec, 2.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288395, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.89, 108.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676420 sequences (100000063 bp) in 1.468 CPU sec, 1.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287822, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.12, 115.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288280, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.22, 112.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676982 reads in 270.121 CPU sec, 8.705 real sec
[M::mem_process_seqs] Processed 676638 reads in 189.197 CPU sec, 6.103 real sec
[M::mem_process_seqs] Processed 676736 reads in 197.071 CPU sec, 6.405 real sec
sorting block 12, #reads = 1082017 mem = 536877657 peak = 944173056
[M::process] read 676144 sequences (100000092 bp) in 2.210 CPU sec, 2.512 real sec...
sorting block 13, #reads = 1082067 mem = 536880207 peak = 944173056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287673, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.66, 116.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676494 sequences (100000015 bp) in 1.824 CPU sec, 1.941 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287685, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.91, 119.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676634 sequences (100000137 bp) in 1.455 CPU sec, 1.458 real sec...
[M::mem_process_seqs] Processed 676420 reads in 187.726 CPU sec, 6.053 real sec
[M::mem_process_seqs] Processed 676144 reads in 106.000 CPU sec, 3.382 real sec
sorting block 14, #reads = 1082256 mem = 536878171 peak = 944173056
[M::process] read 676746 sequences (100000022 bp) in 1.996 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287703, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.80, 114.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676646 sequences (100000145 bp) in 1.447 CPU sec, 1.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288406, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.85, 112.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676494 reads in 182.205 CPU sec, 5.863 real sec
[M::mem_process_seqs] Processed 676634 reads in 104.212 CPU sec, 3.345 real sec
sorting block 15, #reads = 1081794 mem = 536878551 peak = 944173056
sorting block 16, #reads = 1081720 mem = 536879279 peak = 944173056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288358, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.24, 110.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 39, 107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253)
[M::mem_pestat] mean and std.dev: (42.27, 25.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 326)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676492 sequences (100000221 bp) in 1.840 CPU sec, 2.035 real sec...
[M::process] read 676118 sequences (100000000 bp) in 1.452 CPU sec, 1.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288565, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.33, 112.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676746 reads in 182.297 CPU sec, 5.869 real sec
[M::mem_process_seqs] Processed 676646 reads in 102.711 CPU sec, 3.296 real sec
sorting block 17, #reads = 1082482 mem = 536877133 peak = 944173056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288366, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.44, 116.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676048 sequences (100000264 bp) in 1.765 CPU sec, 1.981 real sec...
[M::process] read 676418 sequences (100000033 bp) in 1.469 CPU sec, 1.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287914, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (241.02, 120.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 40, 159)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (53.92, 52.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 534)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676492 reads in 186.671 CPU sec, 6.000 real sec
[M::mem_process_seqs] Processed 676118 reads in 105.785 CPU sec, 3.420 real sec
sorting block 18, #reads = 1082687 mem = 536878094 peak = 944173056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287512, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.61, 121.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 63, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169)
[M::mem_pestat] mean and std.dev: (147.00, 168.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1552)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676794 sequences (100000135 bp) in 1.903 CPU sec, 2.157 real sec...
[M::process] read 676590 sequences (100000011 bp) in 1.422 CPU sec, 1.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287805, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.37, 115.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676048 reads in 187.796 CPU sec, 6.069 real sec
[M::mem_process_seqs] Processed 676418 reads in 104.613 CPU sec, 3.358 real sec
sorting block 19, #reads = 1081965 mem = 536880833 peak = 944173056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288174, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.41, 109.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676372 sequences (100000130 bp) in 1.842 CPU sec, 2.197 real sec...
[M::process] read 676316 sequences (100000224 bp) in 1.460 CPU sec, 1.469 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287479, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.64, 114.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 28, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623)
[M::mem_pestat] mean and std.dev: (352.00, 460.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2193)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676794 reads in 188.736 CPU sec, 6.115 real sec
[M::mem_process_seqs] Processed 676590 reads in 107.963 CPU sec, 3.485 real sec
sorting block 20, #reads = 1081966 mem = 536878185 peak = 957247488
sorting block 21, #reads = 1082158 mem = 536879273 peak = 1031094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287898, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.40, 117.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 17, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509)
[M::mem_pestat] mean and std.dev: (207.07, 263.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2007)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676270 sequences (100000037 bp) in 1.855 CPU sec, 2.128 real sec...
[M::process] read 676908 sequences (100000122 bp) in 1.419 CPU sec, 1.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287758, 18, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.75, 117.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 578, 8118)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24338)
[M::mem_pestat] mean and std.dev: (2830.28, 3747.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 32448)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676372 reads in 189.032 CPU sec, 6.116 real sec
[M::mem_process_seqs] Processed 676316 reads in 106.370 CPU sec, 3.414 real sec
sorting block 22, #reads = 1082100 mem = 536878986 peak = 1031094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287807, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.70, 119.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677110 sequences (100000198 bp) in 1.914 CPU sec, 2.173 real sec...
[M::process] read 676956 sequences (100000288 bp) in 1.512 CPU sec, 1.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288057, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.94, 108.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 12, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 241)
[M::mem_pestat] mean and std.dev: (39.89, 65.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 318)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676270 reads in 188.074 CPU sec, 6.057 real sec
[M::mem_process_seqs] Processed 676908 reads in 103.974 CPU sec, 3.332 real sec
sorting block 23, #reads = 1081386 mem = 536878345 peak = 1031094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288404, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.39, 106.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (346, 1818, 1920)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5068)
[M::mem_pestat] mean and std.dev: (1422.15, 899.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6642)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676504 sequences (100000183 bp) in 2.034 CPU sec, 2.332 real sec...
[M::process] read 676490 sequences (100000218 bp) in 1.468 CPU sec, 1.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288077, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.36, 105.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677110 reads in 186.018 CPU sec, 6.044 real sec
[M::mem_process_seqs] Processed 676956 reads in 103.766 CPU sec, 3.402 real sec
sorting block 24, #reads = 1082688 mem = 536879155 peak = 1031094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288171, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.03, 110.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676478 sequences (100000130 bp) in 2.162 CPU sec, 2.562 real sec...
[M::process] read 676436 sequences (100000162 bp) in 1.488 CPU sec, 1.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288023, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.44, 113.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676504 reads in 188.794 CPU sec, 6.108 real sec
[M::mem_process_seqs] Processed 676490 reads in 107.912 CPU sec, 3.444 real sec
sorting block 25, #reads = 1082669 mem = 536881786 peak = 1031094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287879, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
sorting block 26, #reads = 1082428 mem = 536881767 peak = 1031094272
[M::mem_pestat] mean and std.dev: (234.59, 114.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676752 sequences (100000239 bp) in 1.899 CPU sec, 2.370 real sec...
[M::process] read 676778 sequences (100000242 bp) in 1.438 CPU sec, 1.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288225, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.65, 114.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676478 reads in 185.784 CPU sec, 6.053 real sec
[M::mem_process_seqs] Processed 676436 reads in 103.363 CPU sec, 3.381 real sec
sorting block 27, #reads = 1082239 mem = 536880156 peak = 1031094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288402, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.96, 109.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 69, 3714)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11130)
[M::mem_pestat] mean and std.dev: (1370.55, 1771.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14838)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676556 sequences (100000011 bp) in 1.914 CPU sec, 2.104 real sec...
[M::process] read 676418 sequences (100000228 bp) in 1.577 CPU sec, 1.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288445, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (229.02, 109.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 92, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (131.56, 191.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676752 reads in 185.651 CPU sec, 6.007 real sec
[M::mem_process_seqs] Processed 676778 reads in 104.859 CPU sec, 3.391 real sec
sorting block 28, #reads = 1082667 mem = 536878730 peak = 1031094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288135, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.52, 113.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676072 sequences (100000241 bp) in 2.106 CPU sec, 2.374 real sec...
[M::process] read 676270 sequences (100000136 bp) in 1.508 CPU sec, 1.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287803, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.84, 115.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676556 reads in 187.887 CPU sec, 6.061 real sec
[M::mem_process_seqs] Processed 676418 reads in 105.514 CPU sec, 3.395 real sec
sorting block 29, #reads = 1082706 mem = 536880725 peak = 1031094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287753, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (239.04, 119.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 28, 92)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 228)
[M::mem_pestat] mean and std.dev: (52.91, 54.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 296)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676938 sequences (100000016 bp) in 2.006 CPU sec, 2.358 real sec...
[M::process] read 676884 sequences (100000043 bp) in 1.415 CPU sec, 1.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287671, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.77, 119.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676072 reads in 188.017 CPU sec, 6.108 real sec
[M::mem_process_seqs] Processed 676270 reads in 105.555 CPU sec, 3.418 real sec
sorting block 30, #reads = 1081862 mem = 536880007 peak = 1031094272
sorting block 31, #reads = 1081731 mem = 536879758 peak = 1031094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287975, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.13, 112.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676598 sequences (100000087 bp) in 1.898 CPU sec, 2.312 real sec...
[M::process] read 676404 sequences (100000262 bp) in 1.453 CPU sec, 1.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287905, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.85, 114.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 116, 990)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2888)
[M::mem_pestat] mean and std.dev: (441.36, 535.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3837)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676938 reads in 186.351 CPU sec, 6.037 real sec
[M::mem_process_seqs] Processed 676884 reads in 104.482 CPU sec, 3.384 real sec
sorting block 32, #reads = 1082348 mem = 536878365 peak = 1031094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287745, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.03, 115.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676220 sequences (100000018 bp) in 1.886 CPU sec, 2.142 real sec...
[M::process] read 676190 sequences (100000055 bp) in 1.577 CPU sec, 1.588 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287852, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.51, 118.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676598 reads in 190.504 CPU sec, 6.150 real sec
[M::mem_process_seqs] Processed 676404 reads in 107.844 CPU sec, 3.471 real sec
sorting block 33, #reads = 1082384 mem = 536878530 peak = 1031094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287260, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (242.95, 123.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676334 sequences (100000272 bp) in 2.059 CPU sec, 2.353 real sec...
[M::process] read 676906 sequences (100000152 bp) in 1.507 CPU sec, 1.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287249, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.21, 123.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676220 reads in 190.493 CPU sec, 6.124 real sec
[M::mem_process_seqs] Processed 676190 reads in 106.090 CPU sec, 3.382 real sec
sorting block 34, #reads = 1082137 mem = 536877223 peak = 1031094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288006, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.41, 120.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677060 sequences (100000157 bp) in 2.330 CPU sec, 2.593 real sec...
[M::process] read 676808 sequences (100000052 bp) in 1.458 CPU sec, 1.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288292, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.51, 110.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676334 reads in 188.708 CPU sec, 6.058 real sec
[M::mem_process_seqs] Processed 676906 reads in 104.851 CPU sec, 3.344 real sec
sorting block 35, #reads = 1081918 mem = 536879581 peak = 1031094272
sorting block 36, #reads = 1082598 mem = 536877370 peak = 1055744000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287494, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.21, 109.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 92, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (158.67, 181.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1360)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676652 sequences (100000155 bp) in 2.032 CPU sec, 2.265 real sec...
[M::process] read 676624 sequences (100000222 bp) in 1.513 CPU sec, 1.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287834, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.42, 111.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677060 reads in 191.387 CPU sec, 6.155 real sec
[M::mem_process_seqs] Processed 676808 reads in 106.944 CPU sec, 3.409 real sec
sorting block 37, #reads = 1082252 mem = 536878847 peak = 1055744000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288104, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.68, 113.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676734 sequences (100000096 bp) in 1.917 CPU sec, 2.197 real sec...
[M::process] read 676696 sequences (100000033 bp) in 1.562 CPU sec, 1.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287580, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.14, 114.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 610, 1974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5764)
[M::mem_pestat] mean and std.dev: (1068.91, 1285.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7659)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676652 reads in 187.679 CPU sec, 6.040 real sec
sorting block 38, #reads = 1082327 mem = 536878035 peak = 1055744000
[M::process] read 676588 sequences (100000015 bp) in 1.505 CPU sec, 1.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287903, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.56, 113.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 656, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1753)
[M::mem_pestat] mean and std.dev: (374.00, 295.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2289)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676734 reads in 98.962 CPU sec, 3.181 real sec
[M::mem_process_seqs] Processed 676624 reads in 242.388 CPU sec, 7.809 real sec
sorting block 39, #reads = 1081631 mem = 536877968 peak = 1055744000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287234, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.55, 115.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676624 sequences (100000178 bp) in 2.009 CPU sec, 2.324 real sec...
[M::process] read 676466 sequences (100000163 bp) in 1.521 CPU sec, 1.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287869, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.01, 114.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676696 reads in 196.019 CPU sec, 6.312 real sec
[M::mem_process_seqs] Processed 676588 reads in 106.372 CPU sec, 3.412 real sec
sorting block 40, #reads = 1081506 mem = 536881924 peak = 1055744000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287761, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.33, 114.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 600, 3636)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10844)
[M::mem_pestat] mean and std.dev: (1135.00, 1481.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14448)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676164 sequences (100000170 bp) in 2.411 CPU sec, 2.632 real sec...
[M::process] read 676444 sequences (100000106 bp) in 1.514 CPU sec, 1.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286859, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.84, 120.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676624 reads in 192.224 CPU sec, 6.177 real sec
[M::mem_process_seqs] Processed 676466 reads in 108.251 CPU sec, 3.481 real sec
sorting block 41, #reads = 1081889 mem = 536878902 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286625, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (242.99, 124.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 75, 89)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187)
[M::mem_pestat] mean and std.dev: (64.79, 27.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 236)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676756 sequences (100000232 bp) in 1.988 CPU sec, 2.345 real sec...
[M::process] read 677242 sequences (100000193 bp) in 1.471 CPU sec, 1.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286836, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.59, 120.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (523, 558, 976)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882)
[M::mem_pestat] mean and std.dev: (714.67, 554.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2931)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676164 reads in 195.815 CPU sec, 6.336 real sec
[M::mem_process_seqs] Processed 676444 reads in 109.655 CPU sec, 3.579 real sec
sorting block 42, #reads = 1081335 mem = 536881355 peak = 1068646400
sorting block 43, #reads = 1081455 mem = 536877280 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287298, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.28, 111.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 210, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1658)
[M::mem_pestat] mean and std.dev: (244.78, 231.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2182)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676876 sequences (100000270 bp) in 2.239 CPU sec, 2.532 real sec...
[M::process] read 676734 sequences (100000018 bp) in 1.537 CPU sec, 1.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288401, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.74, 103.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 100, 2335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6827)
[M::mem_pestat] mean and std.dev: (749.00, 1038.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9073)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676756 reads in 193.360 CPU sec, 6.243 real sec
[M::mem_process_seqs] Processed 677242 reads in 106.511 CPU sec, 3.423 real sec
sorting block 44, #reads = 1083087 mem = 536879698 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287788, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.30, 109.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676832 sequences (100000221 bp) in 2.126 CPU sec, 2.365 real sec...
[M::process] read 676852 sequences (100000257 bp) in 1.582 CPU sec, 1.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287542, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.04, 112.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676876 reads in 190.537 CPU sec, 6.155 real sec
[M::mem_process_seqs] Processed 676734 reads in 104.919 CPU sec, 3.432 real sec
sorting block 45, #reads = 1082563 mem = 536878789 peak = 1068646400
[M::process] read 676810 sequences (100000012 bp) in 2.179 CPU sec, 2.490 real sec...
[M::process] read 677106 sequences (100000215 bp) in 1.402 CPU sec, 1.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287642, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (227.95, 111.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676852 reads in 97.710 CPU sec, 3.155 real sec
sorting block 46, #reads = 1082119 mem = 536879110 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287615, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.06, 113.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676980 sequences (100000116 bp) in 1.468 CPU sec, 1.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288009, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.69, 110.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676832 reads in 289.082 CPU sec, 9.354 real sec
[M::mem_process_seqs] Processed 676810 reads in 115.343 CPU sec, 3.764 real sec
sorting block 47, #reads = 1081768 mem = 536877483 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287709, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (229.67, 111.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676864 sequences (100000291 bp) in 2.167 CPU sec, 2.471 real sec...
[M::process] read 676468 sequences (100000030 bp) in 1.505 CPU sec, 1.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288132, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.57, 108.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677106 reads in 191.578 CPU sec, 6.181 real sec
[M::mem_process_seqs] Processed 676980 reads in 108.051 CPU sec, 3.472 real sec
sorting block 48, #reads = 1082783 mem = 536878377 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287778, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.47, 112.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676296 sequences (100000069 bp) in 1.965 CPU sec, 2.224 real sec...
[M::process] read 676470 sequences (100000189 bp) in 1.490 CPU sec, 1.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287456, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.96, 117.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 73, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (100.00, 130.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676864 reads in 190.172 CPU sec, 6.135 real sec
[M::mem_process_seqs] Processed 676468 reads in 107.619 CPU sec, 3.467 real sec
sorting block 49, #reads = 1082301 mem = 536880125 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286897, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.15, 120.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 71, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (214.53, 269.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 50, #reads = 1082119 mem = 536879156 peak = 1068646400
[M::process] read 677100 sequences (100000194 bp) in 1.975 CPU sec, 2.317 real sec...
[M::process] read 676954 sequences (100000163 bp) in 1.541 CPU sec, 1.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287621, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.36, 117.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676296 reads in 196.297 CPU sec, 6.361 real sec
[M::mem_process_seqs] Processed 676470 reads in 109.592 CPU sec, 3.530 real sec
sorting block 51, #reads = 1081969 mem = 536878991 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288226, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.32, 101.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676778 sequences (100000144 bp) in 1.848 CPU sec, 2.254 real sec...
[M::process] read 676822 sequences (100000294 bp) in 1.360 CPU sec, 1.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287745, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.96, 111.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677100 reads in 189.431 CPU sec, 6.136 real sec
[M::mem_process_seqs] Processed 676954 reads in 107.199 CPU sec, 3.442 real sec
sorting block 52, #reads = 1082934 mem = 536880576 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287794, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.16, 110.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (448, 726, 2322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6070)
[M::mem_pestat] mean and std.dev: (1002.64, 847.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7944)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676704 sequences (100000293 bp) in 1.851 CPU sec, 2.298 real sec...
[M::process] read 677288 sequences (100000132 bp) in 1.541 CPU sec, 1.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287678, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.02, 113.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676778 reads in 193.049 CPU sec, 6.247 real sec
[M::mem_process_seqs] Processed 676822 reads in 108.185 CPU sec, 3.470 real sec
sorting block 53, #reads = 1082586 mem = 536876253 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287842, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (231.99, 115.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (286, 797, 3108)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8752)
[M::mem_pestat] mean and std.dev: (1167.27, 1220.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11574)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677172 sequences (100000202 bp) in 1.850 CPU sec, 2.226 real sec...
[M::process] read 677228 sequences (100000109 bp) in 1.530 CPU sec, 1.534 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288049, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.40, 109.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 176, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1292)
[M::mem_pestat] mean and std.dev: (216.67, 230.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1710)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676704 reads in 187.664 CPU sec, 6.061 real sec
[M::mem_process_seqs] Processed 677288 reads in 105.368 CPU sec, 3.393 real sec
sorting block 54, #reads = 1082165 mem = 536878096 peak = 1068646400
sorting block 55, #reads = 1081118 mem = 536877768 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288241, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.40, 105.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676962 sequences (100000025 bp) in 1.951 CPU sec, 2.198 real sec...
[M::process] read 676950 sequences (100000051 bp) in 1.558 CPU sec, 1.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288153, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.63, 105.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677172 reads in 186.583 CPU sec, 6.014 real sec
[M::mem_process_seqs] Processed 677228 reads in 106.354 CPU sec, 3.409 real sec
sorting block 56, #reads = 1081904 mem = 536879477 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287390, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676586 sequences (100000021 bp) in 1.965 CPU sec, 2.155 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.29, 108.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676770 sequences (100000059 bp) in 1.516 CPU sec, 1.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287625, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.48, 110.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676962 reads in 193.346 CPU sec, 6.246 real sec
[M::mem_process_seqs] Processed 676950 reads in 108.722 CPU sec, 3.536 real sec
sorting block 57, #reads = 1081172 mem = 536878502 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287059, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.97, 114.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677048 sequences (100000265 bp) in 1.969 CPU sec, 2.279 real sec...
[M::process] read 676988 sequences (100000124 bp) in 1.391 CPU sec, 1.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287705, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.92, 114.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676586 reads in 196.413 CPU sec, 6.340 real sec
[M::mem_process_seqs] Processed 676770 reads in 109.432 CPU sec, 3.514 real sec
sorting block 58, #reads = 1082304 mem = 536879405 peak = 1068646400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288003, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.03, 108.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676786 sequences (100000055 bp) in 1.886 CPU sec, 2.211 real sec...
[M::process] read 676634 sequences (100000270 bp) in 1.511 CPU sec, 1.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288302, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.73, 107.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677048 reads in 185.038 CPU sec, 5.962 real sec
[M::mem_process_seqs] Processed 676988 reads in 103.684 CPU sec, 3.317 real sec
sorting block 59, #reads = 1081908 mem = 536879035 peak = 1068646400
sorting block 60, #reads = 1082457 mem = 536877812 peak = 1071558656
[M::process] read 676554 sequences (100000231 bp) in 1.792 CPU sec, 2.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287819, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.42, 112.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 556422 sequences (82247563 bp) in 1.354 CPU sec, 1.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287741, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.33, 115.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676786 reads in 188.202 CPU sec, 6.056 real sec
[M::mem_process_seqs] Processed 676634 reads in 106.467 CPU sec, 3.414 real sec
sorting block 61, #reads = 1082210 mem = 536880940 peak = 1071558656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287429, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.58, 118.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 89, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (57, 112)
[M::mem_pestat] mean and std.dev: (86.56, 4.17)
[M::mem_pestat] low and high boundaries for proper pairs: (46, 123)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 236534, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (232.84, 116.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676554 reads in 146.645 CPU sec, 4.776 real sec
[M::mem_process_seqs] Processed 556422 reads in 66.496 CPU sec, 2.163 real sec
sorting block 62, #reads = 1082198 mem = 536883766 peak = 1071558656
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 68216860
overall: 69736857600 mem 11531.630 user 424.163 sys 403.129 real
sorting block 63, #reads = 564981 mem = 280417744 peak = 1071558656
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L004\tPU:L004\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.T21-D0003376_R1.fastq.gz 0003.T21-D0003376_R2.fastq.gz
[main] Real time: 403.192 sec; CPU: 11955.823 sec
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2648051761
output reads: 68738668
bam_mem_sort: 64 calls 43.442 user 1.141 sys 46.020 real
bam_write: 64 calls 36.825 user 2.801 sys 73.800 real
execute: 1 calls 17.619 user 1.039 sys 422.951 real
merge_files: 1 calls 17.607 user 1.001 sys 19.333 real
parse_chunk: 1017 calls 46.021 user 12.453 sys 69.592 real
read_chunk: 2738 calls 22.099 user 12.845 sys 330.176 real
sort_block: 1 calls 15.663 user 12.144 sys 320.948 real
write_chunk: 1719 calls 17.302 user 0.826 sys 18.589 real
overall: 2402344960 mem 684.612 user 44.212 sys 422.972 real
ls: cannot access '*.cram': No such file or directory