Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/42/4d5e554c2e25374f01128eb892af84/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/04/de7278d15d6d8ce661e5e0f6f63913/T21-D0003376/0004.T21-D0003376_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/04/de7278d15d6d8ce661e5e0f6f63913/T21-D0003376/0004.T21-D0003376_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/42/4d5e554c2e25374f01128eb892af84/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2805.609 CPU sec, 113.077 real sec
[M::process] read 677706 sequences (100000178 bp) in 1.366 CPU sec, 1.369 real sec...
[M::process] read 677614 sequences (100000165 bp) in 1.798 CPU sec, 1.834 real sec...
[M::process] read 677202 sequences (100000250 bp) in 1.550 CPU sec, 1.568 real sec...
[M::process] read 677166 sequences (100000120 bp) in 1.558 CPU sec, 1.600 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289298, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (214.49, 92.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289092, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (218.20, 97.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288401, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.68, 101.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 87, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064)
[M::mem_pestat] mean and std.dev: (234.08, 301.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1439)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677706 reads in 371.664 CPU sec, 12.272 real sec
[M::mem_process_seqs] Processed 677614 reads in 228.987 CPU sec, 7.717 real sec
[M::mem_process_seqs] Processed 677202 reads in 243.273 CPU sec, 8.233 real sec
sorting block 0, #reads = 1083282 mem = 536877208 peak = 594743296
[M::process] read 677134 sequences (100000064 bp) in 2.323 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288392, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.18, 104.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677006 sequences (100000152 bp) in 1.979 CPU sec, 2.201 real sec...
[M::process] read 676892 sequences (100000221 bp) in 1.323 CPU sec, 1.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288387, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.76, 106.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677166 reads in 224.220 CPU sec, 7.225 real sec
sorting block 1, #reads = 1082820 mem = 536878735 peak = 802361344
[M::mem_process_seqs] Processed 677134 reads in 132.622 CPU sec, 4.276 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288453, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.72, 109.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1082306 mem = 536878456 peak = 873914368
[M::process] read 676680 sequences (100000265 bp) in 2.283 CPU sec, 2.460 real sec...
[M::process] read 676586 sequences (100000151 bp) in 1.585 CPU sec, 1.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288105, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.40, 109.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677006 reads in 221.176 CPU sec, 7.158 real sec
[M::mem_process_seqs] Processed 676892 reads in 128.703 CPU sec, 4.176 real sec
sorting block 3, #reads = 1082075 mem = 536879496 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288411, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.87, 111.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676556 sequences (100000259 bp) in 2.329 CPU sec, 2.674 real sec...
[M::process] read 676458 sequences (100000181 bp) in 1.270 CPU sec, 1.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288186, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.84, 111.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 67, 800)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2344)
[M::mem_pestat] mean and std.dev: (305.42, 480.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3116)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676680 reads in 221.033 CPU sec, 7.179 real sec
[M::mem_process_seqs] Processed 676586 reads in 128.495 CPU sec, 4.201 real sec
sorting block 4, #reads = 1082718 mem = 536878631 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288159, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.14, 114.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676306 sequences (100000054 bp) in 2.218 CPU sec, 2.530 real sec...
[M::process] read 676794 sequences (100000174 bp) in 1.772 CPU sec, 1.782 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287871, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.44, 116.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676556 reads in 218.928 CPU sec, 7.156 real sec
[M::mem_process_seqs] Processed 676458 reads in 127.605 CPU sec, 4.216 real sec
sorting block 5, #reads = 1082465 mem = 536879108 peak = 873914368
[M::process] read 677550 sequences (100000265 bp) in 1.946 CPU sec, 2.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288102, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.75, 117.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677450 sequences (100000148 bp) in 1.315 CPU sec, 1.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288242, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.16, 112.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676306 reads in 217.003 CPU sec, 7.031 real sec
sorting block 6, #reads = 1081722 mem = 536877720 peak = 873914368
[M::mem_process_seqs] Processed 676794 reads in 127.160 CPU sec, 4.133 real sec
sorting block 7, #reads = 1081734 mem = 536878961 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288927, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (219.12, 98.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677220 sequences (100000064 bp) in 2.541 CPU sec, 2.871 real sec...
[M::process] read 677118 sequences (100000204 bp) in 1.633 CPU sec, 1.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288812, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (221.01, 101.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677550 reads in 221.779 CPU sec, 7.138 real sec
[M::mem_process_seqs] Processed 677450 reads in 130.078 CPU sec, 4.199 real sec
sorting block 8, #reads = 1082786 mem = 536878151 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288626, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (223.27, 103.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676952 sequences (100000079 bp) in 2.070 CPU sec, 2.409 real sec...
[M::process] read 677018 sequences (100000224 bp) in 1.441 CPU sec, 1.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288667, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.64, 106.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677220 reads in 221.000 CPU sec, 7.361 real sec
[M::mem_process_seqs] Processed 677118 reads in 127.268 CPU sec, 4.390 real sec
sorting block 9, #reads = 1082258 mem = 536877705 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288325, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.32, 108.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676870 sequences (100000124 bp) in 2.549 CPU sec, 2.914 real sec...
[M::process] read 676632 sequences (100000283 bp) in 1.876 CPU sec, 1.905 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288869, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.02, 108.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676952 reads in 221.272 CPU sec, 7.329 real sec
[M::mem_process_seqs] Processed 677018 reads in 129.816 CPU sec, 4.285 real sec
sorting block 10, #reads = 1082595 mem = 536878377 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288715, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.79, 111.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676576 sequences (100000033 bp) in 2.239 CPU sec, 2.570 real sec...
[M::process] read 676322 sequences (100000192 bp) in 1.555 CPU sec, 1.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288400, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.38, 112.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676870 reads in 220.982 CPU sec, 7.150 real sec
sorting block 11, #reads = 1082968 mem = 536878748 peak = 873914368
[M::mem_process_seqs] Processed 676632 reads in 129.377 CPU sec, 4.178 real sec
sorting block 12, #reads = 1082663 mem = 536883792 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288291, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.88, 114.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 214, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136)
[M::mem_pestat] mean and std.dev: (248.86, 294.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1502)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676334 sequences (100000148 bp) in 2.651 CPU sec, 3.112 real sec...
[M::process] read 676168 sequences (100000033 bp) in 1.729 CPU sec, 1.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288131, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (236.14, 116.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676576 reads in 225.905 CPU sec, 7.289 real sec
[M::mem_process_seqs] Processed 676322 reads in 133.106 CPU sec, 4.281 real sec
sorting block 13, #reads = 1082400 mem = 536881356 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287974, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.65, 118.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677364 sequences (100000128 bp) in 2.475 CPU sec, 2.901 real sec...
[M::process] read 677498 sequences (100000169 bp) in 1.612 CPU sec, 1.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288145, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.29, 120.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676334 reads in 223.589 CPU sec, 7.246 real sec
[M::mem_process_seqs] Processed 676168 reads in 133.555 CPU sec, 4.366 real sec
sorting block 14, #reads = 1082197 mem = 536879637 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288756, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.36, 105.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677338 sequences (100000053 bp) in 2.730 CPU sec, 3.292 real sec...
[M::process] read 677194 sequences (100000146 bp) in 1.687 CPU sec, 1.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288396, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (219.83, 99.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677364 reads in 226.655 CPU sec, 7.344 real sec
[M::mem_process_seqs] Processed 677498 reads in 136.394 CPU sec, 4.430 real sec
sorting block 15, #reads = 1081880 mem = 536878692 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288275, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.38, 102.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677076 sequences (100000184 bp) in 2.799 CPU sec, 3.286 real sec...
sorting block 16, #reads = 1082701 mem = 536878735 peak = 873914368
[M::process] read 676852 sequences (100000059 bp) in 1.502 CPU sec, 1.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288146, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (225.20, 105.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677338 reads in 222.569 CPU sec, 7.176 real sec
[M::mem_process_seqs] Processed 677194 reads in 129.353 CPU sec, 4.187 real sec
sorting block 17, #reads = 1082432 mem = 536880356 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288176, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.71, 107.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 20, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1595)
[M::mem_pestat] mean and std.dev: (155.50, 238.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2123)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676822 sequences (100000123 bp) in 2.430 CPU sec, 2.757 real sec...
[M::process] read 676808 sequences (100000116 bp) in 1.826 CPU sec, 1.832 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288233, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.60, 109.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677076 reads in 227.127 CPU sec, 7.328 real sec
[M::mem_process_seqs] Processed 676852 reads in 132.035 CPU sec, 4.293 real sec
sorting block 18, #reads = 1082489 mem = 536880864 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288211, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.16, 109.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676666 sequences (100000061 bp) in 2.300 CPU sec, 2.694 real sec...
[M::process] read 676524 sequences (100000122 bp) in 1.585 CPU sec, 1.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288021, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.03, 112.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676822 reads in 225.641 CPU sec, 7.353 real sec
[M::mem_process_seqs] Processed 676808 reads in 134.058 CPU sec, 4.362 real sec
sorting block 19, #reads = 1082698 mem = 536881192 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288569, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.28, 113.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676402 sequences (100000199 bp) in 2.421 CPU sec, 2.710 real sec...
[M::process] read 676154 sequences (100000232 bp) in 1.599 CPU sec, 1.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287969, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.69, 115.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676666 reads in 228.315 CPU sec, 7.375 real sec
[M::mem_process_seqs] Processed 676524 reads in 133.856 CPU sec, 4.329 real sec
sorting block 20, #reads = 1082163 mem = 536878718 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288057, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.73, 116.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676578 sequences (100000196 bp) in 2.644 CPU sec, 3.005 real sec...
sorting block 21, #reads = 1082904 mem = 536879985 peak = 873914368
[M::process] read 677654 sequences (100000197 bp) in 1.644 CPU sec, 1.721 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287905, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.53, 119.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676402 reads in 228.998 CPU sec, 7.409 real sec
[M::mem_process_seqs] Processed 676154 reads in 134.810 CPU sec, 4.390 real sec
sorting block 22, #reads = 1082241 mem = 536877896 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287762, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.28, 117.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 34, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (117.80, 219.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677518 sequences (100000167 bp) in 2.632 CPU sec, 3.020 real sec...
[M::process] read 677236 sequences (100000033 bp) in 1.821 CPU sec, 1.821 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288979, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (217.05, 95.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676578 reads in 225.733 CPU sec, 7.318 real sec
[M::mem_process_seqs] Processed 677654 reads in 131.419 CPU sec, 4.280 real sec
sorting block 23, #reads = 1081522 mem = 536878248 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288301, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (220.12, 99.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676746 sequences (100000265 bp) in 2.438 CPU sec, 2.781 real sec...
[M::process] read 677034 sequences (100000081 bp) in 1.704 CPU sec, 1.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288225, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (223.41, 103.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677518 reads in 232.332 CPU sec, 7.510 real sec
[M::mem_process_seqs] Processed 677236 reads in 139.698 CPU sec, 4.564 real sec
sorting block 24, #reads = 1082290 mem = 536882132 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288373, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.59, 110.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676778 sequences (100000067 bp) in 2.882 CPU sec, 3.316 real sec...
[M::process] read 676552 sequences (100000150 bp) in 1.562 CPU sec, 1.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288265, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.68, 107.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676746 reads in 230.022 CPU sec, 7.453 real sec
[M::mem_process_seqs] Processed 677034 reads in 137.669 CPU sec, 4.473 real sec
sorting block 25, #reads = 1082555 mem = 536877578 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287830, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.12, 108.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 23, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1235)
[M::mem_pestat] mean and std.dev: (203.50, 332.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1643)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 26, #reads = 1082797 mem = 536879767 peak = 873914368
[M::process] read 676666 sequences (100000235 bp) in 2.652 CPU sec, 3.063 real sec...
[M::process] read 676458 sequences (100000142 bp) in 1.904 CPU sec, 1.946 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288262, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.85, 112.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676778 reads in 223.408 CPU sec, 7.311 real sec
[M::mem_process_seqs] Processed 676552 reads in 125.828 CPU sec, 4.143 real sec
sorting block 27, #reads = 1082212 mem = 536879388 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288793, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.00, 111.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676560 sequences (100000044 bp) in 2.383 CPU sec, 2.830 real sec...
[M::process] read 676296 sequences (100000101 bp) in 1.904 CPU sec, 1.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287873, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.80, 115.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676666 reads in 223.052 CPU sec, 7.265 real sec
[M::mem_process_seqs] Processed 676458 reads in 126.305 CPU sec, 4.083 real sec
sorting block 28, #reads = 1082386 mem = 536876542 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288277, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.41, 115.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676130 sequences (100000238 bp) in 2.586 CPU sec, 3.101 real sec...
[M::process] read 676852 sequences (100000003 bp) in 1.986 CPU sec, 2.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287798, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.56, 119.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676560 reads in 223.059 CPU sec, 7.231 real sec
[M::mem_process_seqs] Processed 676296 reads in 126.940 CPU sec, 4.066 real sec
sorting block 29, #reads = 1081733 mem = 536878334 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287842, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (240.06, 119.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 40, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1197)
[M::mem_pestat] mean and std.dev: (85.12, 127.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1585)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677812 sequences (100000212 bp) in 2.455 CPU sec, 3.035 real sec...
[M::process] read 677652 sequences (100000230 bp) in 1.640 CPU sec, 1.646 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288212, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.98, 111.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676130 reads in 223.075 CPU sec, 7.237 real sec
[M::mem_process_seqs] Processed 676852 reads in 126.272 CPU sec, 4.061 real sec
sorting block 30, #reads = 1082007 mem = 536880110 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288758, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (214.70, 94.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677472 sequences (100000202 bp) in 2.742 CPU sec, 3.281 real sec...
sorting block 31, #reads = 1081752 mem = 536877364 peak = 873914368
[M::process] read 677418 sequences (100000096 bp) in 1.659 CPU sec, 1.778 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288631, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (215.91, 96.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677812 reads in 223.328 CPU sec, 7.235 real sec
[M::mem_process_seqs] Processed 677652 reads in 127.427 CPU sec, 4.089 real sec
sorting block 32, #reads = 1082767 mem = 536877475 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288772, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (218.20, 98.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677364 sequences (100000257 bp) in 2.387 CPU sec, 2.846 real sec...
[M::process] read 677152 sequences (100000111 bp) in 1.813 CPU sec, 1.824 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288453, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.32, 101.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677472 reads in 225.859 CPU sec, 7.312 real sec
[M::mem_process_seqs] Processed 677418 reads in 126.991 CPU sec, 4.062 real sec
sorting block 33, #reads = 1083191 mem = 536877630 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288198, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (222.96, 104.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677006 sequences (100000056 bp) in 2.286 CPU sec, 2.922 real sec...
[M::process] read 676902 sequences (100000022 bp) in 1.835 CPU sec, 1.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288097, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.32, 106.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 887, 9544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28482)
[M::mem_pestat] mean and std.dev: (3045.41, 3797.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 37951)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677364 reads in 224.004 CPU sec, 7.257 real sec
[M::mem_process_seqs] Processed 677152 reads in 127.377 CPU sec, 4.095 real sec
sorting block 34, #reads = 1082521 mem = 536879544 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288717, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.61, 108.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676836 sequences (100000137 bp) in 2.319 CPU sec, 2.771 real sec...
[M::process] read 676808 sequences (100000177 bp) in 1.808 CPU sec, 1.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288063, 20, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.33, 111.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (59, 83, 167)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383)
[M::mem_pestat] mean and std.dev: (68.69, 46.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 491)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677006 reads in 226.163 CPU sec, 7.337 real sec
[M::mem_process_seqs] Processed 676902 reads in 126.261 CPU sec, 4.059 real sec
sorting block 35, #reads = 1083090 mem = 536878408 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288062, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.80, 111.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1082442 mem = 536879628 peak = 873914368
[M::process] read 676626 sequences (100000160 bp) in 2.742 CPU sec, 3.234 real sec...
[M::process] read 676610 sequences (100000238 bp) in 1.674 CPU sec, 1.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288313, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.72, 113.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676836 reads in 224.356 CPU sec, 7.247 real sec
[M::mem_process_seqs] Processed 676808 reads in 124.307 CPU sec, 3.979 real sec
sorting block 37, #reads = 1082623 mem = 536876699 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287836, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.88, 115.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677872 sequences (100000097 bp) in 2.457 CPU sec, 2.807 real sec...
[M::process] read 677608 sequences (100000084 bp) in 2.121 CPU sec, 2.234 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287661, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (232.80, 115.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 62, 1110)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3248)
[M::mem_pestat] mean and std.dev: (232.80, 346.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4317)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676626 reads in 225.179 CPU sec, 7.253 real sec
[M::mem_process_seqs] Processed 676610 reads in 129.293 CPU sec, 4.145 real sec
sorting block 38, #reads = 1082271 mem = 536879423 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288647, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (208.30, 85.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 621)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677514 sequences (100000008 bp) in 2.516 CPU sec, 3.067 real sec...
[M::process] read 677468 sequences (100000204 bp) in 1.683 CPU sec, 1.728 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288654, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (215.45, 94.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 40, 173)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449)
[M::mem_pestat] mean and std.dev: (83.00, 106.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 587)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677872 reads in 220.241 CPU sec, 7.161 real sec
[M::mem_process_seqs] Processed 677608 reads in 125.744 CPU sec, 4.088 real sec
sorting block 39, #reads = 1081910 mem = 536880039 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288697, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.33, 100.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677310 sequences (100000246 bp) in 2.635 CPU sec, 2.983 real sec...
[M::process] read 677246 sequences (100000146 bp) in 1.665 CPU sec, 1.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288216, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.32, 101.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677514 reads in 224.313 CPU sec, 7.241 real sec
sorting block 40, #reads = 1082602 mem = 536878766 peak = 873914368
[M::mem_process_seqs] Processed 677468 reads in 125.964 CPU sec, 4.023 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288289, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (222.14, 104.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1082770 mem = 536878703 peak = 873914368
[M::process] read 677174 sequences (100000223 bp) in 3.153 CPU sec, 3.700 real sec...
[M::process] read 676880 sequences (100000225 bp) in 1.799 CPU sec, 1.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288324, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.66, 103.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677310 reads in 226.882 CPU sec, 7.307 real sec
[M::mem_process_seqs] Processed 677246 reads in 127.792 CPU sec, 4.091 real sec
sorting block 42, #reads = 1082245 mem = 536880494 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288108, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.29, 107.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676964 sequences (100000215 bp) in 2.489 CPU sec, 2.928 real sec...
[M::process] read 676698 sequences (100000291 bp) in 1.901 CPU sec, 1.965 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287912, 22, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.53, 110.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 330, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1731)
[M::mem_pestat] mean and std.dev: (345.77, 347.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2304)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677174 reads in 233.518 CPU sec, 7.553 real sec
[M::mem_process_seqs] Processed 676880 reads in 133.646 CPU sec, 4.319 real sec
sorting block 43, #reads = 1082166 mem = 536879872 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287798, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.06, 111.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676698 sequences (100000271 bp) in 2.746 CPU sec, 3.191 real sec...
[M::process] read 676714 sequences (100000206 bp) in 1.653 CPU sec, 1.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287863, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.34, 113.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676964 reads in 227.298 CPU sec, 7.340 real sec
[M::mem_process_seqs] Processed 676698 reads in 128.203 CPU sec, 4.104 real sec
sorting block 44, #reads = 1081674 mem = 536879291 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288134, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.32, 113.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677648 sequences (100000240 bp) in 2.810 CPU sec, 3.260 real sec...
sorting block 45, #reads = 1082512 mem = 536880310 peak = 873914368
[M::process] read 677634 sequences (100000202 bp) in 1.659 CPU sec, 1.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287949, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.19, 116.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676698 reads in 227.013 CPU sec, 7.324 real sec
[M::mem_process_seqs] Processed 676714 reads in 126.881 CPU sec, 4.055 real sec
sorting block 46, #reads = 1082507 mem = 536881701 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288380, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (217.20, 98.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677558 sequences (100000272 bp) in 2.704 CPU sec, 3.258 real sec...
[M::process] read 677354 sequences (100000038 bp) in 1.901 CPU sec, 1.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288576, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558)
[M::mem_pestat] mean and std.dev: (217.10, 97.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677648 reads in 221.791 CPU sec, 7.158 real sec
[M::mem_process_seqs] Processed 677634 reads in 125.116 CPU sec, 3.997 real sec
sorting block 47, #reads = 1082086 mem = 536877114 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288362, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.29, 100.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677334 sequences (100000030 bp) in 2.456 CPU sec, 2.860 real sec...
[M::process] read 677098 sequences (100000079 bp) in 1.728 CPU sec, 1.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288455, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.49, 104.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677558 reads in 224.900 CPU sec, 7.260 real sec
[M::mem_process_seqs] Processed 677354 reads in 125.896 CPU sec, 4.044 real sec
sorting block 48, #reads = 1082862 mem = 536878876 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288531, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.65, 105.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677182 sequences (100000204 bp) in 2.828 CPU sec, 3.246 real sec...
[M::process] read 676994 sequences (100000150 bp) in 1.793 CPU sec, 1.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288064, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.15, 107.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 39, 79)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 209)
[M::mem_pestat] mean and std.dev: (48.40, 58.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 284)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677334 reads in 224.605 CPU sec, 7.228 real sec
[M::mem_process_seqs] Processed 677098 reads in 126.055 CPU sec, 4.028 real sec
sorting block 49, #reads = 1082782 mem = 536880467 peak = 873914368
sorting block 50, #reads = 1082242 mem = 536879805 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288172, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.38, 106.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 19, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205)
[M::mem_pestat] mean and std.dev: (33.31, 37.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 272)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676878 sequences (100000263 bp) in 2.343 CPU sec, 2.526 real sec...
[M::process] read 676816 sequences (100000065 bp) in 1.418 CPU sec, 1.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288168, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.36, 109.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (100, 684, 2982)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8746)
[M::mem_pestat] mean and std.dev: (1239.10, 1415.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11628)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677182 reads in 201.654 CPU sec, 6.476 real sec
[M::mem_process_seqs] Processed 676994 reads in 107.612 CPU sec, 3.429 real sec
sorting block 51, #reads = 1082441 mem = 536877849 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288194, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.93, 111.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 58, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 143)
[M::mem_pestat] mean and std.dev: (43.38, 20.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 179)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676658 sequences (100000239 bp) in 2.269 CPU sec, 2.502 real sec...
[M::process] read 676566 sequences (100000090 bp) in 1.409 CPU sec, 1.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288145, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.09, 112.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676878 reads in 195.622 CPU sec, 6.262 real sec
[M::mem_process_seqs] Processed 676816 reads in 109.172 CPU sec, 3.494 real sec
sorting block 52, #reads = 1082645 mem = 536877063 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288009, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.21, 115.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677320 sequences (100000129 bp) in 2.384 CPU sec, 2.578 real sec...
[M::process] read 677914 sequences (100000094 bp) in 1.640 CPU sec, 1.641 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287878, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (232.95, 116.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676658 reads in 204.817 CPU sec, 6.566 real sec
[M::mem_process_seqs] Processed 676566 reads in 114.571 CPU sec, 3.655 real sec
sorting block 53, #reads = 1082635 mem = 536879491 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288491, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (222.96, 105.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677510 sequences (100000248 bp) in 2.310 CPU sec, 2.510 real sec...
[M::process] read 677196 sequences (100000035 bp) in 1.510 CPU sec, 1.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288932, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (214.90, 94.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 29, 34)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 58)
[M::mem_pestat] mean and std.dev: (25.44, 9.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 70)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677320 reads in 192.831 CPU sec, 6.171 real sec
[M::mem_process_seqs] Processed 677914 reads in 108.317 CPU sec, 3.454 real sec
sorting block 54, #reads = 1082452 mem = 536879267 peak = 873914368
sorting block 55, #reads = 1082759 mem = 536878968 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288495, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (217.89, 98.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (403, 1121, 1588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3958)
[M::mem_pestat] mean and std.dev: (880.00, 725.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5143)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677210 sequences (100000159 bp) in 2.386 CPU sec, 2.567 real sec...
[M::process] read 676990 sequences (100000234 bp) in 1.720 CPU sec, 1.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288117, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.12, 109.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677510 reads in 199.958 CPU sec, 6.386 real sec
[M::mem_process_seqs] Processed 677196 reads in 114.298 CPU sec, 3.652 real sec
sorting block 56, #reads = 1082986 mem = 536878832 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288285, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (223.89, 105.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676934 sequences (100000190 bp) in 2.594 CPU sec, 2.806 real sec...
[M::process] read 676948 sequences (100000163 bp) in 1.749 CPU sec, 1.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288095, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.97, 107.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677210 reads in 205.894 CPU sec, 6.578 real sec
[M::mem_process_seqs] Processed 676990 reads in 114.557 CPU sec, 3.649 real sec
sorting block 57, #reads = 1082575 mem = 536881794 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287591, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (227.91, 109.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676904 sequences (100000235 bp) in 2.391 CPU sec, 2.587 real sec...
[M::process] read 676700 sequences (100000164 bp) in 1.499 CPU sec, 1.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287642, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.27, 109.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676934 reads in 208.329 CPU sec, 6.664 real sec
[M::mem_process_seqs] Processed 676948 reads in 112.753 CPU sec, 3.599 real sec
sorting block 58, #reads = 1082449 mem = 536879094 peak = 873914368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287749, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.74, 113.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 91, 1110)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3148)
[M::mem_pestat] mean and std.dev: (324.42, 449.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4167)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676574 sequences (100000036 bp) in 2.228 CPU sec, 2.411 real sec...
[M::process] read 676360 sequences (100000152 bp) in 1.320 CPU sec, 1.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287604, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.45, 114.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2542, 3290, 3290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1046, 4786)
[M::mem_pestat] mean and std.dev: (3165.33, 278.76)
[M::mem_pestat] low and high boundaries for proper pairs: (298, 5534)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676904 reads in 192.456 CPU sec, 6.151 real sec
[M::mem_process_seqs] Processed 676700 reads in 106.464 CPU sec, 3.401 real sec
sorting block 59, #reads = 1082243 mem = 536878024 peak = 873914368
sorting block 60, #reads = 1082146 mem = 536881456 peak = 873914368
[M::process] read 563004 sequences (83219762 bp) in 1.968 CPU sec, 2.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287825, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (234.95, 117.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 72, 119)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275)
[M::mem_pestat] mean and std.dev: (56.88, 32.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 353)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287551, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.37, 120.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 239127, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.98, 117.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676574 reads in 242.173 CPU sec, 7.803 real sec
[M::mem_process_seqs] Processed 676360 reads in 155.824 CPU sec, 5.038 real sec
[M::mem_process_seqs] Processed 563004 reads in 161.790 CPU sec, 5.245 real sec
sorting block 61, #reads = 1081634 mem = 536882251 peak = 873914368
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67585154
overall: 69165887488 mem 13190.989 user 1164.037 sys 497.477 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L006\tPU:L006\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.T21-D0003376_R1.fastq.gz 0004.T21-D0003376_R2.fastq.gz
[main] Real time: 497.507 sec; CPU: 14355.030 sec
sorting block 62, #reads = 1000268 mem = 496273653 peak = 873914368
uniq header count 1
merging 63 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2650538443
output reads: 68110247
bam_mem_sort: 63 calls 60.570 user 1.908 sys 63.332 real
bam_write: 63 calls 42.757 user 4.135 sys 74.144 real
execute: 1 calls 14.642 user 0.647 sys 514.353 real
merge_files: 1 calls 14.632 user 0.607 sys 15.842 real
parse_chunk: 1007 calls 48.648 user 14.902 sys 73.525 real
read_chunk: 2712 calls 31.925 user 16.284 sys 379.143 real
sort_block: 1 calls 28.043 user 15.713 sys 371.758 real
write_chunk: 1703 calls 14.296 user 0.354 sys 14.982 real
overall: 2023387136 mem 700.464 user 56.203 sys 514.370 real
ls: cannot access '*.cram': No such file or directory