This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 3294.350 CPU sec, 133.879 real sec
[M::process] read 677730 sequences (100000025 bp) in 1.330 CPU sec, 1.338 real sec...
[M::process] read 677472 sequences (100000286 bp) in 1.779 CPU sec, 1.812 real sec...
[M::process] read 677272 sequences (100000027 bp) in 1.909 CPU sec, 1.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289233, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (214.87, 93.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677086 sequences (100000006 bp) in 1.774 CPU sec, 1.865 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288540, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (218.01, 97.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289285, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (222.02, 102.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677730 reads in 400.040 CPU sec, 13.615 real sec
[M::mem_process_seqs] Processed 677472 reads in 252.315 CPU sec, 8.882 real sec
[M::mem_process_seqs] Processed 677272 reads in 261.069 CPU sec, 9.222 real sec
sorting block 0, #reads = 1083048 mem = 536879677 peak = 574382080
[M::process] read 676994 sequences (100000118 bp) in 2.057 CPU sec, 2.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288203, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.64, 103.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676854 sequences (100000211 bp) in 1.913 CPU sec, 2.050 real sec...
[M::process] read 676716 sequences (100000197 bp) in 1.586 CPU sec, 1.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288526, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.42, 107.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677086 reads in 243.565 CPU sec, 7.847 real sec
[M::mem_process_seqs] Processed 676994 reads in 133.988 CPU sec, 4.324 real sec
sorting block 1, #reads = 1082895 mem = 536879505 peak = 826040320
sorting block 2, #reads = 1082651 mem = 536877130 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288387, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.09, 108.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676916 sequences (100000266 bp) in 2.619 CPU sec, 2.907 real sec...
[M::process] read 676668 sequences (100000045 bp) in 1.499 CPU sec, 1.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288428, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.83, 109.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 71, 79)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187)
[M::mem_pestat] mean and std.dev: (45.36, 28.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 241)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676854 reads in 245.808 CPU sec, 8.001 real sec
[M::mem_process_seqs] Processed 676716 reads in 135.317 CPU sec, 4.438 real sec
sorting block 3, #reads = 1083026 mem = 536878907 peak = 882315264
[M::process] read 676454 sequences (100000270 bp) in 2.278 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288973, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.82, 111.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 79, 628)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870)
[M::mem_pestat] mean and std.dev: (267.92, 287.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2491)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676356 sequences (100000259 bp) in 1.447 CPU sec, 1.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287933, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.00, 113.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287797, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.37, 112.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676916 reads in 361.296 CPU sec, 11.748 real sec
[M::mem_process_seqs] Processed 676454 reads in 250.089 CPU sec, 8.191 real sec
[M::mem_process_seqs] Processed 676668 reads in 258.817 CPU sec, 8.556 real sec
sorting block 4, #reads = 1082599 mem = 536881472 peak = 882315264
[M::process] read 676286 sequences (100000097 bp) in 2.163 CPU sec, 2.429 real sec...
sorting block 5, #reads = 1082088 mem = 536878854 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288225, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.09, 116.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 167, 1378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3956)
[M::mem_pestat] mean and std.dev: (602.92, 642.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5245)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676714 sequences (100000155 bp) in 2.168 CPU sec, 2.363 real sec...
[M::process] read 677560 sequences (100000080 bp) in 1.530 CPU sec, 1.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288257, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.22, 118.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676356 reads in 251.765 CPU sec, 8.276 real sec
[M::mem_process_seqs] Processed 676286 reads in 139.884 CPU sec, 4.592 real sec
sorting block 6, #reads = 1081955 mem = 536879366 peak = 882315264
[M::process] read 677470 sequences (100000182 bp) in 2.248 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287960, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.65, 112.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677350 sequences (100000054 bp) in 1.429 CPU sec, 1.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288977, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (217.94, 96.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288902, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.92, 101.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 54, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (143.40, 181.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676714 reads in 343.561 CPU sec, 11.475 real sec
[M::mem_process_seqs] Processed 677560 reads in 228.073 CPU sec, 7.799 real sec
[M::mem_process_seqs] Processed 677470 reads in 237.201 CPU sec, 8.192 real sec
sorting block 7, #reads = 1081808 mem = 536880853 peak = 882315264
[M::process] read 677170 sequences (100000029 bp) in 1.938 CPU sec, 2.242 real sec...
sorting block 8, #reads = 1082686 mem = 536879974 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288540, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (223.02, 103.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676916 sequences (100000189 bp) in 2.703 CPU sec, 2.997 real sec...
[M::process] read 676882 sequences (100000234 bp) in 1.507 CPU sec, 1.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288385, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.46, 106.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677350 reads in 228.314 CPU sec, 7.652 real sec
[M::mem_process_seqs] Processed 677170 reads in 127.973 CPU sec, 4.195 real sec
sorting block 9, #reads = 1082659 mem = 536878646 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287933, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.14, 108.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 49, 584)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1682)
[M::mem_pestat] mean and std.dev: (171.40, 236.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2231)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676848 sequences (100000183 bp) in 2.626 CPU sec, 3.067 real sec...
[M::process] read 676710 sequences (100000232 bp) in 1.437 CPU sec, 1.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288653, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.08, 108.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676916 reads in 228.400 CPU sec, 7.414 real sec
[M::mem_process_seqs] Processed 676882 reads in 125.349 CPU sec, 4.093 real sec
sorting block 10, #reads = 1082619 mem = 536880071 peak = 882315264
[M::process] read 676584 sequences (100000152 bp) in 2.253 CPU sec, 2.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287931, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.24, 110.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676304 sequences (100000108 bp) in 1.570 CPU sec, 1.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288060, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.23, 113.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 417, 666)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852)
[M::mem_pestat] mean and std.dev: (510.86, 482.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2445)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676848 reads in 227.138 CPU sec, 7.379 real sec
sorting block 11, #reads = 1082573 mem = 536880950 peak = 882315264
[M::mem_process_seqs] Processed 676710 reads in 125.305 CPU sec, 4.104 real sec
sorting block 12, #reads = 1082270 mem = 536878633 peak = 882315264
[M::process] read 676486 sequences (100000024 bp) in 2.147 CPU sec, 2.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287947, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.92, 114.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (720, 720, 1738)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3774)
[M::mem_pestat] mean and std.dev: (1076.18, 1005.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5098)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676240 sequences (100000158 bp) in 1.524 CPU sec, 1.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287646, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.89, 116.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676584 reads in 223.078 CPU sec, 7.218 real sec
[M::mem_process_seqs] Processed 676304 reads in 124.602 CPU sec, 4.038 real sec
sorting block 13, #reads = 1082222 mem = 536878866 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288139, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.39, 118.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677228 sequences (100000058 bp) in 2.433 CPU sec, 2.724 real sec...
[M::process] read 677376 sequences (100000012 bp) in 1.593 CPU sec, 1.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287925, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.95, 120.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 145, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059)
[M::mem_pestat] mean and std.dev: (241.50, 242.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1399)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676486 reads in 223.310 CPU sec, 7.221 real sec
[M::mem_process_seqs] Processed 676240 reads in 123.048 CPU sec, 3.972 real sec
sorting block 14, #reads = 1082372 mem = 536879340 peak = 882315264
[M::process] read 677366 sequences (100000231 bp) in 1.963 CPU sec, 2.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288603, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.71, 104.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677346 sequences (100000161 bp) in 1.494 CPU sec, 1.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288544, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.43, 98.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677228 reads in 223.363 CPU sec, 7.225 real sec
[M::mem_process_seqs] Processed 677376 reads in 126.146 CPU sec, 4.097 real sec
sorting block 15, #reads = 1081486 mem = 536879154 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288302, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (222.04, 102.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1082670 mem = 536879379 peak = 882315264
[M::process] read 677076 sequences (100000103 bp) in 2.643 CPU sec, 2.980 real sec...
[M::process] read 676998 sequences (100000250 bp) in 1.637 CPU sec, 1.648 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288326, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.35, 104.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 444, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1252)
[M::mem_pestat] mean and std.dev: (225.62, 192.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1656)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677366 reads in 227.253 CPU sec, 7.345 real sec
[M::mem_process_seqs] Processed 677346 reads in 127.887 CPU sec, 4.121 real sec
sorting block 17, #reads = 1082560 mem = 536879438 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288247, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.66, 107.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 27, 34)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 92)
[M::mem_pestat] mean and std.dev: (17.67, 14.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 121)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676932 sequences (100000290 bp) in 2.455 CPU sec, 2.842 real sec...
[M::process] read 676814 sequences (100000111 bp) in 1.688 CPU sec, 1.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288258, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.81, 108.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677076 reads in 227.111 CPU sec, 7.397 real sec
[M::mem_process_seqs] Processed 676998 reads in 128.060 CPU sec, 4.215 real sec
sorting block 18, #reads = 1082465 mem = 536880696 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288537, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.89, 109.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 389, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155)
[M::mem_pestat] mean and std.dev: (329.38, 368.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1804)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676656 sequences (100000087 bp) in 2.812 CPU sec, 3.210 real sec...
[M::process] read 676590 sequences (100000172 bp) in 1.669 CPU sec, 1.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288039, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.54, 112.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676932 reads in 229.492 CPU sec, 7.474 real sec
[M::mem_process_seqs] Processed 676814 reads in 129.539 CPU sec, 4.208 real sec
sorting block 19, #reads = 1082445 mem = 536881932 peak = 882315264
[M::process] read 676310 sequences (100000188 bp) in 2.020 CPU sec, 2.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288466, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.67, 113.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 11, 34)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 100)
[M::mem_pestat] mean and std.dev: (11.00, 12.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 133)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676328 sequences (100000166 bp) in 1.370 CPU sec, 1.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287827, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.97, 114.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676656 reads in 230.832 CPU sec, 7.488 real sec
[M::mem_process_seqs] Processed 676590 reads in 130.551 CPU sec, 4.240 real sec
sorting block 20, #reads = 1082492 mem = 536880390 peak = 882315264
[M::process] read 676516 sequences (100000152 bp) in 2.269 CPU sec, 2.550 real sec...
sorting block 21, #reads = 1082650 mem = 536877390 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287790, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.53, 117.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677634 sequences (100000145 bp) in 1.631 CPU sec, 1.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288161, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.30, 119.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676310 reads in 230.076 CPU sec, 7.467 real sec
[M::mem_process_seqs] Processed 676328 reads in 127.176 CPU sec, 4.150 real sec
sorting block 22, #reads = 1082384 mem = 536878086 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287899, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.31, 118.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677370 sequences (100000157 bp) in 2.218 CPU sec, 2.517 real sec...
[M::process] read 677188 sequences (100000130 bp) in 1.458 CPU sec, 1.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288434, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (216.91, 95.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676516 reads in 232.956 CPU sec, 7.559 real sec
[M::mem_process_seqs] Processed 677634 reads in 130.203 CPU sec, 4.215 real sec
sorting block 23, #reads = 1081766 mem = 536876535 peak = 882315264
[M::process] read 676746 sequences (100000201 bp) in 2.484 CPU sec, 2.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288418, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (220.02, 99.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 63, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112)
[M::mem_pestat] mean and std.dev: (134.54, 152.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1480)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676940 sequences (100000078 bp) in 1.497 CPU sec, 1.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288556, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.99, 102.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677370 reads in 227.788 CPU sec, 7.397 real sec
[M::mem_process_seqs] Processed 677188 reads in 128.019 CPU sec, 4.203 real sec
sorting block 24, #reads = 1082592 mem = 536878412 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288270, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.45, 110.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676896 sequences (100000166 bp) in 2.141 CPU sec, 2.762 real sec...
[M::process] read 676552 sequences (100000230 bp) in 1.541 CPU sec, 1.730 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288428, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.17, 107.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676746 reads in 233.130 CPU sec, 7.648 real sec
[M::mem_process_seqs] Processed 676940 reads in 128.388 CPU sec, 4.188 real sec
sorting block 25, #reads = 1082616 mem = 536880423 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287927, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.72, 107.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1082274 mem = 536879259 peak = 882315264
[M::process] read 676704 sequences (100000102 bp) in 2.732 CPU sec, 3.192 real sec...
[M::process] read 676458 sequences (100000294 bp) in 1.757 CPU sec, 1.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288251, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.02, 111.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676896 reads in 222.474 CPU sec, 7.187 real sec
[M::mem_process_seqs] Processed 676552 reads in 125.484 CPU sec, 4.055 real sec
sorting block 27, #reads = 1082458 mem = 536878197 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288225, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.86, 111.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 549, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1584)
[M::mem_pestat] mean and std.dev: (350.54, 273.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2078)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676484 sequences (100000211 bp) in 2.319 CPU sec, 2.654 real sec...
[M::process] read 676330 sequences (100000146 bp) in 1.538 CPU sec, 1.695 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287770, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (235.06, 115.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 549, 1123)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3025)
[M::mem_pestat] mean and std.dev: (547.64, 468.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3976)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676704 reads in 220.358 CPU sec, 7.197 real sec
[M::mem_process_seqs] Processed 676458 reads in 124.004 CPU sec, 4.094 real sec
sorting block 28, #reads = 1082594 mem = 536879081 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288092, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.61, 115.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 45, 2605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7755)
[M::mem_pestat] mean and std.dev: (710.21, 1042.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10330)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676174 sequences (100000073 bp) in 2.574 CPU sec, 3.047 real sec...
[M::process] read 677022 sequences (100000174 bp) in 1.951 CPU sec, 1.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288382, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.08, 119.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676484 reads in 218.683 CPU sec, 7.069 real sec
[M::mem_process_seqs] Processed 676330 reads in 123.976 CPU sec, 3.990 real sec
sorting block 29, #reads = 1081903 mem = 536877231 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287952, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (240.06, 119.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677688 sequences (100000235 bp) in 2.106 CPU sec, 2.634 real sec...
[M::process] read 677652 sequences (100000296 bp) in 1.461 CPU sec, 1.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288634, 14, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.10, 112.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 90, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102)
[M::mem_pestat] mean and std.dev: (214.43, 264.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1467)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676174 reads in 219.337 CPU sec, 7.149 real sec
[M::mem_process_seqs] Processed 677022 reads in 123.018 CPU sec, 3.995 real sec
sorting block 30, #reads = 1082488 mem = 536880085 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288827, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (212.47, 91.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1081796 mem = 536877570 peak = 882315264
[M::process] read 677696 sequences (100000191 bp) in 2.619 CPU sec, 3.112 real sec...
[M::process] read 677328 sequences (100000102 bp) in 1.736 CPU sec, 1.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288664, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (215.63, 95.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 678)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677688 reads in 218.906 CPU sec, 7.101 real sec
[M::mem_process_seqs] Processed 677652 reads in 123.643 CPU sec, 3.992 real sec
sorting block 32, #reads = 1082639 mem = 536879900 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288404, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (217.41, 98.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677224 sequences (100000107 bp) in 2.529 CPU sec, 2.884 real sec...
[M::process] read 677094 sequences (100000071 bp) in 1.676 CPU sec, 1.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288227, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.60, 103.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677696 reads in 225.252 CPU sec, 7.282 real sec
[M::mem_process_seqs] Processed 677328 reads in 128.375 CPU sec, 4.146 real sec
sorting block 33, #reads = 1082824 mem = 536879255 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288118, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.47, 105.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676952 sequences (100000122 bp) in 2.269 CPU sec, 2.673 real sec...
[M::process] read 677008 sequences (100000132 bp) in 1.501 CPU sec, 1.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288470, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.55, 107.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 429, 812)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2384)
[M::mem_pestat] mean and std.dev: (340.50, 348.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3170)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677224 reads in 226.461 CPU sec, 7.348 real sec
[M::mem_process_seqs] Processed 677094 reads in 128.021 CPU sec, 4.144 real sec
sorting block 34, #reads = 1082517 mem = 536878645 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288202, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.79, 108.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676646 sequences (100000099 bp) in 2.162 CPU sec, 2.506 real sec...
[M::process] read 676808 sequences (100000132 bp) in 1.518 CPU sec, 1.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288304, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.55, 110.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676952 reads in 223.510 CPU sec, 7.268 real sec
[M::mem_process_seqs] Processed 677008 reads in 123.836 CPU sec, 4.028 real sec
sorting block 35, #reads = 1083010 mem = 536879505 peak = 882315264
sorting block 36, #reads = 1082184 mem = 536879571 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288352, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.64, 111.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676688 sequences (100000070 bp) in 2.579 CPU sec, 2.787 real sec...
[M::process] read 676558 sequences (100000263 bp) in 1.721 CPU sec, 1.730 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288005, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.06, 113.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (262, 1147, 1147)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2917)
[M::mem_pestat] mean and std.dev: (941.31, 781.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4068)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676646 reads in 223.336 CPU sec, 7.203 real sec
[M::mem_process_seqs] Processed 676808 reads in 123.766 CPU sec, 3.987 real sec
sorting block 37, #reads = 1082911 mem = 536878019 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288210, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.11, 114.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 33, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1674)
[M::mem_pestat] mean and std.dev: (239.76, 261.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2231)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677966 sequences (100000268 bp) in 2.269 CPU sec, 2.562 real sec...
[M::process] read 677674 sequences (100000275 bp) in 1.917 CPU sec, 1.938 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288055, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.39, 116.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676688 reads in 221.600 CPU sec, 7.145 real sec
[M::mem_process_seqs] Processed 676558 reads in 124.809 CPU sec, 4.034 real sec
sorting block 38, #reads = 1082301 mem = 536881028 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288836, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (207.99, 84.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 617)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677514 sequences (100000039 bp) in 2.961 CPU sec, 3.259 real sec...
[M::process] read 677398 sequences (100000009 bp) in 1.728 CPU sec, 1.776 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288929, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (215.61, 95.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 681)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677966 reads in 221.601 CPU sec, 7.207 real sec
[M::mem_process_seqs] Processed 677674 reads in 123.745 CPU sec, 4.038 real sec
sorting block 39, #reads = 1082192 mem = 536879517 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288726, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.21, 100.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677316 sequences (100000273 bp) in 2.455 CPU sec, 2.728 real sec...
[M::process] read 677252 sequences (100000141 bp) in 1.419 CPU sec, 1.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288400, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.37, 101.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677514 reads in 222.895 CPU sec, 7.214 real sec
sorting block 40, #reads = 1082668 mem = 536880199 peak = 882315264
[M::mem_process_seqs] Processed 677398 reads in 125.348 CPU sec, 4.048 real sec
sorting block 41, #reads = 1082606 mem = 536882175 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288384, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677192 sequences (100000112 bp) in 2.434 CPU sec, 2.604 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.31, 104.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 87, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2055)
[M::mem_pestat] mean and std.dev: (372.36, 460.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2733)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677114 sequences (100000173 bp) in 1.802 CPU sec, 1.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287786, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.22, 104.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677316 reads in 224.642 CPU sec, 7.271 real sec
[M::mem_process_seqs] Processed 677252 reads in 126.832 CPU sec, 4.132 real sec
sorting block 42, #reads = 1082561 mem = 536877608 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288358, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (223.98, 106.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676826 sequences (100000176 bp) in 2.704 CPU sec, 3.268 real sec...
[M::process] read 676686 sequences (100000251 bp) in 1.509 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288319, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.69, 109.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677192 reads in 224.662 CPU sec, 7.271 real sec
[M::mem_process_seqs] Processed 677114 reads in 127.236 CPU sec, 4.095 real sec
sorting block 43, #reads = 1082265 mem = 536880208 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288066, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.12, 111.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 145, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (282.82, 313.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1537)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676696 sequences (100000187 bp) in 2.429 CPU sec, 2.806 real sec...
[M::process] read 676648 sequences (100000172 bp) in 1.495 CPU sec, 1.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287656, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.75, 112.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676826 reads in 225.860 CPU sec, 7.327 real sec
[M::mem_process_seqs] Processed 676686 reads in 128.854 CPU sec, 4.158 real sec
sorting block 44, #reads = 1082351 mem = 536880189 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288029, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.78, 114.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 167, 3273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9779)
[M::mem_pestat] mean and std.dev: (988.00, 1356.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13032)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677756 sequences (100000082 bp) in 2.445 CPU sec, 2.857 real sec...
sorting block 45, #reads = 1082359 mem = 536878934 peak = 882315264
[M::process] read 677740 sequences (100000133 bp) in 1.436 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287729, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.53, 114.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676696 reads in 222.241 CPU sec, 7.218 real sec
[M::mem_process_seqs] Processed 676648 reads in 127.067 CPU sec, 4.095 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288740, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (217.13, 98.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1082592 mem = 536877230 peak = 882315264
[M::process] read 677600 sequences (100000128 bp) in 2.595 CPU sec, 2.831 real sec...
[M::process] read 677494 sequences (100000112 bp) in 1.210 CPU sec, 1.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288309, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558)
[M::mem_pestat] mean and std.dev: (216.79, 97.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677756 reads in 205.446 CPU sec, 6.617 real sec
[M::mem_process_seqs] Processed 677740 reads in 110.826 CPU sec, 3.542 real sec
sorting block 47, #reads = 1082538 mem = 536880763 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288466, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.06, 100.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 73, 3555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10563)
[M::mem_pestat] mean and std.dev: (1288.43, 1646.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14067)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677254 sequences (100000123 bp) in 2.360 CPU sec, 2.549 real sec...
[M::process] read 677122 sequences (100000148 bp) in 1.690 CPU sec, 1.687 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288279, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.21, 102.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677600 reads in 201.434 CPU sec, 6.454 real sec
[M::mem_process_seqs] Processed 677494 reads in 113.585 CPU sec, 3.632 real sec
sorting block 48, #reads = 1083073 mem = 536878372 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288149, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.57, 105.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 207, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (171.33, 91.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 727)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677034 sequences (100000156 bp) in 2.424 CPU sec, 2.646 real sec...
[M::process] read 676952 sequences (100000246 bp) in 1.470 CPU sec, 1.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288061, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.57, 106.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677254 reads in 202.512 CPU sec, 6.494 real sec
[M::mem_process_seqs] Processed 677122 reads in 113.855 CPU sec, 3.641 real sec
sorting block 49, #reads = 1082777 mem = 536880405 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288279, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (224.92, 107.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 39, 119)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339)
[M::mem_pestat] mean and std.dev: (34.50, 35.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 449)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 50, #reads = 1082571 mem = 536879696 peak = 882315264
[M::process] read 676918 sequences (100000114 bp) in 2.521 CPU sec, 2.854 real sec...
[M::process] read 676652 sequences (100000041 bp) in 1.770 CPU sec, 1.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288104, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.95, 109.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 186, 186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504)
[M::mem_pestat] mean and std.dev: (135.67, 111.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 663)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677034 reads in 203.698 CPU sec, 6.513 real sec
[M::mem_process_seqs] Processed 676952 reads in 114.563 CPU sec, 3.648 real sec
sorting block 51, #reads = 1082506 mem = 536880376 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287642, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.03, 110.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676660 sequences (100000296 bp) in 2.636 CPU sec, 2.806 real sec...
[M::process] read 676628 sequences (100000158 bp) in 1.685 CPU sec, 1.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287060, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.63, 113.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676918 reads in 211.589 CPU sec, 6.765 real sec
[M::mem_process_seqs] Processed 676652 reads in 119.774 CPU sec, 3.833 real sec
sorting block 52, #reads = 1082762 mem = 536877010 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287805, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (232.73, 115.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677168 sequences (100000212 bp) in 2.553 CPU sec, 2.783 real sec...
[M::process] read 677726 sequences (100000030 bp) in 1.651 CPU sec, 1.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287608, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.23, 116.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676660 reads in 210.777 CPU sec, 6.765 real sec
[M::mem_process_seqs] Processed 676628 reads in 117.702 CPU sec, 3.769 real sec
sorting block 53, #reads = 1082287 mem = 536879666 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288100, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.03, 105.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677610 sequences (100000099 bp) in 2.776 CPU sec, 2.968 real sec...
[M::process] read 677104 sequences (100000085 bp) in 1.640 CPU sec, 1.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288739, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (214.04, 93.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 669)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677168 reads in 205.420 CPU sec, 6.570 real sec
[M::mem_process_seqs] Processed 677726 reads in 113.774 CPU sec, 3.639 real sec
sorting block 54, #reads = 1081544 mem = 536879392 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288638, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (217.47, 97.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1082628 mem = 536880348 peak = 882315264
[M::process] read 677184 sequences (100000172 bp) in 2.594 CPU sec, 2.858 real sec...
[M::process] read 677040 sequences (100000164 bp) in 1.580 CPU sec, 1.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287858, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.06, 108.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677610 reads in 195.580 CPU sec, 6.250 real sec
[M::process] read 676896 sequences (100000189 bp) in 1.781 CPU sec, 1.791 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288566, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.09, 106.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677184 reads in 92.556 CPU sec, 2.942 real sec
sorting block 56, #reads = 1082887 mem = 536878204 peak = 882315264
[M::mem_process_seqs] Processed 677104 reads in 229.788 CPU sec, 7.326 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288042, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.93, 107.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 38, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2028)
[M::mem_pestat] mean and std.dev: (283.69, 382.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2700)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676866 sequences (100000115 bp) in 2.039 CPU sec, 2.137 real sec...
[M::process] read 676748 sequences (100000244 bp) in 1.440 CPU sec, 1.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287971, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.89, 109.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677040 reads in 178.117 CPU sec, 5.672 real sec
sorting block 57, #reads = 1082562 mem = 536879069 peak = 882315264
[M::mem_process_seqs] Processed 676896 reads in 93.972 CPU sec, 2.991 real sec
sorting block 58, #reads = 1082382 mem = 536877358 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288483, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.09, 110.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676660 sequences (100000095 bp) in 2.581 CPU sec, 2.790 real sec...
[M::process] read 676528 sequences (100000017 bp) in 1.530 CPU sec, 1.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288087, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.87, 113.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 1766, 1766)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5262)
[M::mem_pestat] mean and std.dev: (978.36, 812.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7010)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676866 reads in 175.053 CPU sec, 5.575 real sec
[M::mem_process_seqs] Processed 676748 reads in 98.960 CPU sec, 3.166 real sec
sorting block 59, #reads = 1082960 mem = 536878076 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287890, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.84, 113.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 107, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465)
[M::mem_pestat] mean and std.dev: (91.82, 64.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 603)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676416 sequences (100000255 bp) in 2.200 CPU sec, 2.306 real sec...
[M::process] read 565488 sequences (83597307 bp) in 1.430 CPU sec, 1.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287438, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.75, 117.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676660 reads in 184.993 CPU sec, 5.913 real sec
[M::mem_process_seqs] Processed 676528 reads in 106.507 CPU sec, 3.408 real sec
sorting block 60, #reads = 1082526 mem = 536879785 peak = 882315264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287322, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.46, 119.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 240490, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.84, 119.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676416 reads in 163.355 CPU sec, 5.317 real sec
[M::mem_process_seqs] Processed 565488 reads in 83.782 CPU sec, 2.729 real sec
sorting block 61, #reads = 1081306 mem = 536880559 peak = 882315264
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67586940
overall: 69316112384 mem 13753.491 user 1045.780 sys 518.208 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L006\tPU:L006\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.T21-D0003376_R1.fastq.gz 0002.T21-D0003376_R2.fastq.gz
[main] Real time: 518.245 sec; CPU: 14799.282 sec
sorting block 62, #reads = 1000108 mem = 496265394 peak = 882315264
uniq header count 1
merging 63 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2650486216
output reads: 68112507
bam_mem_sort: 63 calls 61.940 user 0.888 sys 63.588 real
bam_write: 63 calls 42.581 user 3.658 sys 74.243 real
execute: 1 calls 13.445 user 0.747 sys 534.053 real
merge_files: 1 calls 13.406 user 0.728 sys 15.123 real
parse_chunk: 1007 calls 47.492 user 15.424 sys 72.725 real
read_chunk: 2712 calls 33.014 user 16.248 sys 377.372 real
sort_block: 1 calls 28.000 user 15.658 sys 370.047 real
write_chunk: 1703 calls 13.080 user 0.497 sys 14.413 real
overall: 2083557376 mem 673.033 user 54.384 sys 534.070 real
ls: cannot access '*.cram': No such file or directory