.command.log - Workdir - DAQ:SENTIEON_BWAMEM (T21-D0003376;L002;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/34/8127bbe29c975e53d43a121f9dffd2/.command.log
Size: 356.9 KB
Task: DAQ:SENTIEON_BWAMEM (T21-D0003376;L002;0001)
Attempt
Content

  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fa/a72f559d1a83254ddc2bf9e7b581bc/T21-D0003376/T21-D0003376_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/34/8127bbe29c975e53d43a121f9dffd2/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fa/a72f559d1a83254ddc2bf9e7b581bc/T21-D0003376/T21-D0003376_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/34/8127bbe29c975e53d43a121f9dffd2/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2300.813 CPU sec, 80.676 real sec
[M::process] read 676988 sequences (100000018 bp) in 1.839 CPU sec, 1.848 real sec...
[M::process] read 676870 sequences (100000129 bp) in 2.126 CPU sec, 2.199 real sec...
[M::process] read 676960 sequences (100000242 bp) in 2.038 CPU sec, 2.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288455, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (227.08, 106.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676636 sequences (100000277 bp) in 1.986 CPU sec, 1.990 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288405, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (229.00, 108.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 13, 45)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 131)
[M::mem_pestat] mean and std.dev: (20.00, 31.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 174)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287960, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.57, 108.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676988 reads in 358.015 CPU sec, 11.620 real sec
[M::mem_process_seqs] Processed 676870 reads in 236.219 CPU sec, 7.739 real sec
[M::mem_process_seqs] Processed 676960 reads in 247.730 CPU sec, 8.140 real sec
sorting block 0, #reads = 1082262 mem = 536881157 peak = 592322560
[M::process] read 676678 sequences (100000268 bp) in 2.578 CPU sec, 2.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287995, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.38, 109.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 190, 2896)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8620)
[M::mem_pestat] mean and std.dev: (905.07, 1261.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11482)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676642 sequences (100000023 bp) in 2.221 CPU sec, 2.314 real sec...
[M::process] read 676572 sequences (100000295 bp) in 1.943 CPU sec, 1.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288255, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.57, 109.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676636 reads in 230.970 CPU sec, 7.449 real sec
sorting block 1, #reads = 1082237 mem = 536877391 peak = 903438336
[M::mem_process_seqs] Processed 676678 reads in 133.729 CPU sec, 4.281 real sec
[M::process] read 676506 sequences (100000071 bp) in 2.178 CPU sec, 2.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288231, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (232.08, 111.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1081902 mem = 536879495 peak = 975323136
[M::process] read 676638 sequences (100000273 bp) in 1.863 CPU sec, 1.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288597, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.92, 112.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 417, 740)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2214)
[M::mem_pestat] mean and std.dev: (352.14, 338.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2951)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676642 reads in 228.593 CPU sec, 7.546 real sec
[M::mem_process_seqs] Processed 676572 reads in 131.139 CPU sec, 4.296 real sec
sorting block 3, #reads = 1082194 mem = 536879736 peak = 975323136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287913, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.44, 113.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676590 sequences (100000159 bp) in 2.428 CPU sec, 2.811 real sec...
[M::process] read 676490 sequences (100000209 bp) in 1.876 CPU sec, 1.895 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288155, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.05, 113.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676506 reads in 235.535 CPU sec, 7.700 real sec
[M::mem_process_seqs] Processed 676638 reads in 132.422 CPU sec, 4.273 real sec
sorting block 4, #reads = 1082266 mem = 536878442 peak = 975323136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287431, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.06, 113.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676468 sequences (100000112 bp) in 2.540 CPU sec, 2.842 real sec...
[M::process] read 676458 sequences (100000052 bp) in 1.907 CPU sec, 1.914 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288274, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.86, 114.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676590 reads in 232.927 CPU sec, 7.511 real sec
[M::mem_process_seqs] Processed 676490 reads in 131.623 CPU sec, 4.222 real sec
sorting block 5, #reads = 1081852 mem = 536879396 peak = 1018327040
[M::process] read 676464 sequences (100000071 bp) in 2.223 CPU sec, 2.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287716, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (235.07, 114.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676576 sequences (100000162 bp) in 1.888 CPU sec, 1.897 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288138, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (236.18, 116.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676468 reads in 231.782 CPU sec, 7.518 real sec
sorting block 6, #reads = 1081829 mem = 536880309 peak = 1028874240
[M::mem_process_seqs] Processed 676458 reads in 131.959 CPU sec, 4.273 real sec
sorting block 7, #reads = 1081766 mem = 536879072 peak = 1037828096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287911, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.20, 115.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 71, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (132.75, 139.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 709)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676446 sequences (100000036 bp) in 2.115 CPU sec, 2.233 real sec...
[M::process] read 676262 sequences (100000241 bp) in 1.930 CPU sec, 1.958 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288111, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.61, 114.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676464 reads in 233.407 CPU sec, 7.665 real sec
[M::mem_process_seqs] Processed 676576 reads in 133.159 CPU sec, 4.358 real sec
sorting block 8, #reads = 1082433 mem = 536877832 peak = 1037828096
[M::process] read 676314 sequences (100000037 bp) in 2.350 CPU sec, 2.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287868, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.79, 116.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676322 sequences (100000198 bp) in 1.910 CPU sec, 1.911 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287467, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.21, 117.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676446 reads in 232.606 CPU sec, 7.497 real sec
[M::mem_process_seqs] Processed 676262 reads in 128.354 CPU sec, 4.105 real sec
sorting block 9, #reads = 1081862 mem = 536880189 peak = 1037828096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287808, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (236.01, 116.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676216 sequences (100000235 bp) in 2.554 CPU sec, 2.843 real sec...
[M::process] read 676190 sequences (100000106 bp) in 1.947 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287954, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.24, 117.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676314 reads in 229.682 CPU sec, 7.496 real sec
[M::mem_process_seqs] Processed 676322 reads in 132.234 CPU sec, 4.261 real sec
sorting block 10, #reads = 1081983 mem = 536878343 peak = 1052971008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286984, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.63, 117.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676126 sequences (100000216 bp) in 2.311 CPU sec, 2.600 real sec...
[M::process] read 676028 sequences (100000287 bp) in 1.796 CPU sec, 1.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287247, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.22, 119.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676216 reads in 233.209 CPU sec, 7.509 real sec
sorting block 11, #reads = 1081997 mem = 536877458 peak = 1060802560
[M::mem_process_seqs] Processed 676190 reads in 132.075 CPU sec, 4.220 real sec
sorting block 12, #reads = 1081741 mem = 536880462 peak = 1072238592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287446, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (240.50, 119.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 187, 3195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9211)
[M::mem_pestat] mean and std.dev: (1497.30, 1694.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12219)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675968 sequences (100000198 bp) in 2.616 CPU sec, 2.805 real sec...
[M::process] read 676082 sequences (100000183 bp) in 1.966 CPU sec, 2.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287270, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.72, 121.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676126 reads in 229.734 CPU sec, 7.439 real sec
[M::mem_process_seqs] Processed 676028 reads in 132.482 CPU sec, 4.266 real sec
sorting block 13, #reads = 1081810 mem = 536878908 peak = 1072238592
[M::process] read 676176 sequences (100000288 bp) in 2.371 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287410, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.50, 120.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676030 sequences (100000015 bp) in 1.909 CPU sec, 1.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286756, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.98, 120.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675968 reads in 231.234 CPU sec, 7.428 real sec
[M::mem_process_seqs] Processed 676082 reads in 133.520 CPU sec, 4.267 real sec
sorting block 14, #reads = 1081689 mem = 536879672 peak = 1072238592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287542, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.81, 120.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676094 sequences (100000225 bp) in 2.291 CPU sec, 2.517 real sec...
[M::process] read 675996 sequences (100000001 bp) in 1.888 CPU sec, 1.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287574, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.55, 120.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676176 reads in 232.976 CPU sec, 7.511 real sec
[M::mem_process_seqs] Processed 676030 reads in 132.091 CPU sec, 4.228 real sec
sorting block 15, #reads = 1081032 mem = 536876789 peak = 1092014080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287575, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.72, 121.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675956 sequences (100000104 bp) in 2.383 CPU sec, 2.695 real sec...
sorting block 16, #reads = 1081774 mem = 536878581 peak = 1092227072
[M::process] read 675930 sequences (100000019 bp) in 1.914 CPU sec, 1.918 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287830, 14, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.09, 121.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 256, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (154.33, 112.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676094 reads in 234.303 CPU sec, 7.605 real sec
[M::mem_process_seqs] Processed 675996 reads in 129.709 CPU sec, 4.166 real sec
sorting block 17, #reads = 1081858 mem = 536879635 peak = 1092227072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287077, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (243.03, 122.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675898 sequences (100000261 bp) in 2.478 CPU sec, 2.833 real sec...
[M::process] read 676044 sequences (100000136 bp) in 1.974 CPU sec, 1.990 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287398, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.57, 123.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675956 reads in 233.321 CPU sec, 7.513 real sec
[M::mem_process_seqs] Processed 675930 reads in 133.394 CPU sec, 4.262 real sec
sorting block 18, #reads = 1081760 mem = 536879112 peak = 1092227072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287549, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.37, 122.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 28, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156)
[M::mem_pestat] mean and std.dev: (126.45, 170.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1532)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676000 sequences (100000212 bp) in 2.569 CPU sec, 2.818 real sec...
[M::process] read 676074 sequences (100000173 bp) in 1.885 CPU sec, 1.900 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287116, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.64, 123.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 78, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (128.60, 113.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675898 reads in 233.362 CPU sec, 7.504 real sec
[M::mem_process_seqs] Processed 676044 reads in 135.991 CPU sec, 4.349 real sec
sorting block 19, #reads = 1081666 mem = 536879371 peak = 1092227072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287458, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (244.38, 124.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676486 sequences (100000100 bp) in 2.721 CPU sec, 2.977 real sec...
[M::process] read 676404 sequences (100000053 bp) in 1.968 CPU sec, 1.972 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287645, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.36, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676000 reads in 232.981 CPU sec, 7.509 real sec
[M::mem_process_seqs] Processed 676074 reads in 131.548 CPU sec, 4.190 real sec
sorting block 20, #reads = 1081325 mem = 536878046 peak = 1099108352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288208, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.23, 115.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1081545 mem = 536876760 peak = 1101049856
[M::process] read 676546 sequences (100000292 bp) in 2.574 CPU sec, 3.026 real sec...
[M::process] read 676316 sequences (100000241 bp) in 1.955 CPU sec, 1.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287391, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.93, 115.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 940, 2350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7022)
[M::mem_pestat] mean and std.dev: (1205.94, 1423.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9358)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676486 reads in 230.207 CPU sec, 7.456 real sec
[M::mem_process_seqs] Processed 676404 reads in 132.311 CPU sec, 4.243 real sec
sorting block 22, #reads = 1082160 mem = 536878194 peak = 1101049856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288337, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.54, 115.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 12, 89)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255)
[M::mem_pestat] mean and std.dev: (29.08, 34.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 338)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676508 sequences (100000087 bp) in 2.347 CPU sec, 2.483 real sec...
[M::process] read 676352 sequences (100000297 bp) in 1.997 CPU sec, 2.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287733, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (235.07, 116.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676546 reads in 228.345 CPU sec, 7.433 real sec
[M::mem_process_seqs] Processed 676316 reads in 131.133 CPU sec, 4.289 real sec
sorting block 23, #reads = 1082574 mem = 536879649 peak = 1101049856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287812, 15, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.89, 116.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 506, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1368)
[M::mem_pestat] mean and std.dev: (337.73, 214.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1799)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676470 sequences (100000277 bp) in 2.530 CPU sec, 2.883 real sec...
[M::process] read 676464 sequences (100000037 bp) in 2.012 CPU sec, 2.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288086, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.59, 116.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676508 reads in 229.646 CPU sec, 7.442 real sec
[M::mem_process_seqs] Processed 676352 reads in 131.077 CPU sec, 4.211 real sec
sorting block 24, #reads = 1081765 mem = 536878535 peak = 1101049856
[M::process] read 676534 sequences (100000065 bp) in 2.512 CPU sec, 3.107 real sec...
[M::process] read 676482 sequences (100000144 bp) in 1.525 CPU sec, 1.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287633, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.14, 115.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 2170, 2170)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6504)
[M::mem_pestat] mean and std.dev: (1303.20, 1061.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8671)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676464 reads in 120.925 CPU sec, 3.862 real sec
sorting block 25, #reads = 1082743 mem = 536881762 peak = 1119641600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287537, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.74, 116.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287767, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.86, 115.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676318 sequences (100000283 bp) in 1.873 CPU sec, 1.929 real sec...
[M::mem_process_seqs] Processed 676470 reads in 346.654 CPU sec, 11.229 real sec
[M::mem_process_seqs] Processed 676534 reads in 139.407 CPU sec, 4.503 real sec
sorting block 26, #reads = 1081523 mem = 536878113 peak = 1119641600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287693, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (235.01, 116.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676072 sequences (100000040 bp) in 2.731 CPU sec, 3.258 real sec...
[M::process] read 676272 sequences (100000120 bp) in 1.919 CPU sec, 1.938 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287516, 22, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.20, 117.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 473, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (272.58, 183.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1331)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676482 reads in 225.311 CPU sec, 7.253 real sec
[M::mem_process_seqs] Processed 676318 reads in 130.232 CPU sec, 4.136 real sec
sorting block 27, #reads = 1082116 mem = 536876895 peak = 1119641600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288025, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.65, 118.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 86, 176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (83.90, 73.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676120 sequences (100000294 bp) in 2.526 CPU sec, 2.681 real sec...
[M::process] read 676148 sequences (100000032 bp) in 1.912 CPU sec, 1.919 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287958, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.44, 119.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 34, 1073)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3187)
[M::mem_pestat] mean and std.dev: (410.60, 486.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4244)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676072 reads in 221.928 CPU sec, 7.137 real sec
sorting block 28, #reads = 1082037 mem = 536879160 peak = 1145757696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288016, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.32, 118.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676308 sequences (100000023 bp) in 2.124 CPU sec, 2.132 real sec...
[M::mem_process_seqs] Processed 676120 reads in 117.978 CPU sec, 3.766 real sec
sorting block 29, #reads = 1082229 mem = 536879971 peak = 1145757696
[M::mem_process_seqs] Processed 676272 reads in 291.656 CPU sec, 9.346 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287571, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.82, 119.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676864 sequences (100000249 bp) in 2.702 CPU sec, 2.865 real sec...
[M::process] read 676694 sequences (100000212 bp) in 1.945 CPU sec, 1.953 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287489, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.80, 117.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 8, 51)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 137)
[M::mem_pestat] mean and std.dev: (14.31, 15.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 180)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676148 reads in 228.484 CPU sec, 7.304 real sec
[M::mem_process_seqs] Processed 676308 reads in 121.406 CPU sec, 3.856 real sec
sorting block 30, #reads = 1081843 mem = 536878078 peak = 1147731968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288517, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.06, 109.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1081474 mem = 536879371 peak = 1147731968
[M::process] read 676772 sequences (100000063 bp) in 2.782 CPU sec, 3.182 real sec...
[M::process] read 676682 sequences (100000280 bp) in 1.883 CPU sec, 1.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288505, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.36, 109.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676864 reads in 220.972 CPU sec, 7.152 real sec
[M::mem_process_seqs] Processed 676694 reads in 126.916 CPU sec, 4.061 real sec
sorting block 32, #reads = 1082248 mem = 536881282 peak = 1147731968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288104, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.81, 111.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676724 sequences (100000087 bp) in 2.523 CPU sec, 2.637 real sec...
[M::process] read 676568 sequences (100000081 bp) in 1.976 CPU sec, 2.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288194, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.41, 110.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 30, 75)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 165)
[M::mem_pestat] mean and std.dev: (51.00, 35.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 210)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676772 reads in 218.356 CPU sec, 7.062 real sec
[M::mem_process_seqs] Processed 676682 reads in 125.947 CPU sec, 4.032 real sec
sorting block 33, #reads = 1082328 mem = 536879599 peak = 1147731968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288374, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.34, 113.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676456 sequences (100000283 bp) in 2.883 CPU sec, 3.321 real sec...
[M::process] read 676526 sequences (100000187 bp) in 2.141 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288403, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.94, 112.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676724 reads in 218.073 CPU sec, 7.014 real sec
[M::mem_process_seqs] Processed 676568 reads in 124.998 CPU sec, 3.951 real sec
sorting block 34, #reads = 1082136 mem = 536879030 peak = 1153605632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288456, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.44, 113.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676480 sequences (100000250 bp) in 2.740 CPU sec, 3.252 real sec...
[M::process] read 676292 sequences (100000180 bp) in 2.007 CPU sec, 2.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288026, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.79, 115.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676456 reads in 221.603 CPU sec, 7.162 real sec
sorting block 35, #reads = 1082440 mem = 536877987 peak = 1188909056
[M::mem_process_seqs] Processed 676526 reads in 127.445 CPU sec, 4.045 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288135, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.30, 116.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1082114 mem = 536879631 peak = 1188909056
[M::process] read 676458 sequences (100000022 bp) in 2.814 CPU sec, 3.150 real sec...
[M::process] read 676388 sequences (100000059 bp) in 2.001 CPU sec, 2.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288162, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.98, 116.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 51, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (102.50, 98.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676480 reads in 225.745 CPU sec, 7.325 real sec
[M::mem_process_seqs] Processed 676292 reads in 129.439 CPU sec, 4.142 real sec
sorting block 37, #reads = 1082258 mem = 536880551 peak = 1188909056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287918, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.76, 115.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676432 sequences (100000233 bp) in 2.901 CPU sec, 3.197 real sec...
[M::process] read 676522 sequences (100000207 bp) in 1.880 CPU sec, 1.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288135, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.96, 117.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676458 reads in 217.286 CPU sec, 6.978 real sec
[M::mem_process_seqs] Processed 676388 reads in 120.388 CPU sec, 3.823 real sec
sorting block 38, #reads = 1081782 mem = 536877829 peak = 1188909056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288128, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.23, 116.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 125, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (184.10, 217.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676526 sequences (100000235 bp) in 2.940 CPU sec, 3.247 real sec...
[M::process] read 676354 sequences (100000137 bp) in 2.079 CPU sec, 2.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288525, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.56, 116.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676432 reads in 219.940 CPU sec, 7.104 real sec
sorting block 39, #reads = 1082106 mem = 536878225 peak = 1188909056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287851, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.15, 116.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676394 sequences (100000159 bp) in 2.330 CPU sec, 2.429 real sec...
[M::mem_process_seqs] Processed 676526 reads in 120.518 CPU sec, 3.905 real sec
[M::mem_process_seqs] Processed 676522 reads in 298.426 CPU sec, 9.620 real sec
sorting block 40, #reads = 1081971 mem = 536877549 peak = 1188909056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287996, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.85, 117.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676360 sequences (100000143 bp) in 2.701 CPU sec, 3.102 real sec...
[M::process] read 676296 sequences (100000196 bp) in 2.056 CPU sec, 2.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287553, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.34, 118.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676354 reads in 238.666 CPU sec, 7.698 real sec
[M::mem_process_seqs] Processed 676394 reads in 129.468 CPU sec, 4.145 real sec
sorting block 41, #reads = 1082449 mem = 536879100 peak = 1188909056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287925, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.66, 118.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676118 sequences (100000271 bp) in 2.690 CPU sec, 2.936 real sec...
[M::process] read 676110 sequences (100000090 bp) in 2.062 CPU sec, 2.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287348, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.91, 119.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676360 reads in 228.030 CPU sec, 7.360 real sec
[M::mem_process_seqs] Processed 676296 reads in 132.940 CPU sec, 4.306 real sec
sorting block 42, #reads = 1082512 mem = 536878648 peak = 1202655232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287517, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.59, 121.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 43, #reads = 1081701 mem = 536877707 peak = 1204453376
[M::process] read 676074 sequences (100000175 bp) in 2.935 CPU sec, 3.151 real sec...
[M::process] read 676010 sequences (100000145 bp) in 1.975 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287635, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.24, 122.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 9, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1255)
[M::mem_pestat] mean and std.dev: (155.00, 199.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1673)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676118 reads in 230.163 CPU sec, 7.467 real sec
[M::mem_process_seqs] Processed 676110 reads in 132.563 CPU sec, 4.273 real sec
sorting block 44, #reads = 1081817 mem = 536878395 peak = 1204453376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287306, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.95, 121.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 91, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (80.73, 78.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676082 sequences (100000168 bp) in 2.588 CPU sec, 2.773 real sec...
[M::process] read 676044 sequences (100000038 bp) in 2.054 CPU sec, 2.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287848, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.28, 123.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676074 reads in 222.642 CPU sec, 7.134 real sec
[M::mem_process_seqs] Processed 676010 reads in 124.613 CPU sec, 3.946 real sec
sorting block 45, #reads = 1081955 mem = 536879442 peak = 1204453376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287504, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.14, 122.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676012 sequences (100000139 bp) in 2.763 CPU sec, 3.206 real sec...
[M::process] read 675972 sequences (100000216 bp) in 2.051 CPU sec, 2.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287548, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.48, 122.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676082 reads in 228.869 CPU sec, 7.463 real sec
[M::mem_process_seqs] Processed 676044 reads in 130.729 CPU sec, 4.244 real sec
sorting block 46, #reads = 1081463 mem = 536878784 peak = 1204453376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287668, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.69, 122.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676098 sequences (100000207 bp) in 2.675 CPU sec, 3.059 real sec...
[M::process] read 676092 sequences (100000000 bp) in 2.119 CPU sec, 2.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288134, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.47, 122.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 90, 981)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2841)
[M::mem_pestat] mean and std.dev: (381.39, 470.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3771)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676012 reads in 226.130 CPU sec, 7.289 real sec
[M::mem_process_seqs] Processed 675972 reads in 130.994 CPU sec, 4.176 real sec
sorting block 47, #reads = 1082380 mem = 536880485 peak = 1204453376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287309, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.69, 121.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 178, 965)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2793)
[M::mem_pestat] mean and std.dev: (431.73, 390.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3707)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 48, #reads = 1081794 mem = 536877998 peak = 1204453376
[M::process] read 675958 sequences (100000143 bp) in 2.815 CPU sec, 2.961 real sec...
[M::process] read 675862 sequences (100000153 bp) in 1.918 CPU sec, 1.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287987, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (244.37, 124.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676098 reads in 228.234 CPU sec, 7.347 real sec
[M::mem_process_seqs] Processed 676092 reads in 129.374 CPU sec, 4.134 real sec
sorting block 49, #reads = 1081906 mem = 536880114 peak = 1204453376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287287, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (244.85, 124.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675884 sequences (100000170 bp) in 2.871 CPU sec, 3.194 real sec...
[M::process] read 676202 sequences (100000129 bp) in 2.144 CPU sec, 2.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287801, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (246.11, 125.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675958 reads in 231.883 CPU sec, 7.488 real sec
[M::mem_process_seqs] Processed 675862 reads in 132.906 CPU sec, 4.242 real sec
sorting block 50, #reads = 1081812 mem = 536883044 peak = 1204453376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287134, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (245.42, 125.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 20, 20)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 54)
[M::mem_pestat] mean and std.dev: (12.71, 8.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 71)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676092 sequences (100000010 bp) in 2.816 CPU sec, 3.123 real sec...
[M::process] read 676320 sequences (100000226 bp) in 2.041 CPU sec, 2.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287505, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (245.09, 125.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675884 reads in 231.889 CPU sec, 7.461 real sec
[M::mem_process_seqs] Processed 676202 reads in 133.559 CPU sec, 4.270 real sec
sorting block 51, #reads = 1081832 mem = 536878082 peak = 1215643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287284, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.88, 121.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676418 sequences (100000124 bp) in 2.659 CPU sec, 3.000 real sec...
[M::process] read 676418 sequences (100000144 bp) in 2.155 CPU sec, 2.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287520, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.51, 116.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676092 reads in 230.375 CPU sec, 7.441 real sec
sorting block 52, #reads = 1081448 mem = 536878298 peak = 1225564160
[M::mem_process_seqs] Processed 676320 reads in 131.226 CPU sec, 4.171 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287822, 24, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 53, #reads = 1081815 mem = 536878038 peak = 1225564160
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.22, 117.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 71, 107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261)
[M::mem_pestat] mean and std.dev: (67.35, 42.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 338)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676454 sequences (100000064 bp) in 3.033 CPU sec, 3.218 real sec...
[M::process] read 676552 sequences (100000054 bp) in 2.084 CPU sec, 2.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287874, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.17, 117.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676418 reads in 226.558 CPU sec, 7.249 real sec
[M::mem_process_seqs] Processed 676418 reads in 129.922 CPU sec, 4.117 real sec
sorting block 54, #reads = 1081750 mem = 536878740 peak = 1225564160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287786, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.81, 116.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676280 sequences (100000031 bp) in 2.832 CPU sec, 2.999 real sec...
[M::process] read 676370 sequences (100000257 bp) in 2.161 CPU sec, 2.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287779, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.38, 117.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676454 reads in 231.343 CPU sec, 7.419 real sec
[M::mem_process_seqs] Processed 676552 reads in 130.873 CPU sec, 4.153 real sec
sorting block 55, #reads = 1082521 mem = 536878268 peak = 1225564160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288427, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.38, 119.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676146 sequences (100000160 bp) in 3.005 CPU sec, 3.315 real sec...
[M::process] read 676370 sequences (100000156 bp) in 2.031 CPU sec, 2.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287762, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.48, 118.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676280 reads in 226.898 CPU sec, 7.300 real sec
[M::mem_process_seqs] Processed 676370 reads in 131.158 CPU sec, 4.153 real sec
sorting block 56, #reads = 1082350 mem = 536877721 peak = 1225564160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288000, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.83, 119.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676486 sequences (100000031 bp) in 2.990 CPU sec, 3.229 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288185, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.02, 117.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676416 sequences (100000036 bp) in 2.308 CPU sec, 2.324 real sec...
[M::mem_process_seqs] Processed 676146 reads in 225.448 CPU sec, 7.211 real sec
sorting block 57, #reads = 1082580 mem = 536880736 peak = 1241497600
[M::mem_process_seqs] Processed 676370 reads in 130.101 CPU sec, 4.108 real sec
sorting block 58, #reads = 1082384 mem = 536879427 peak = 1241497600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288416, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.29, 118.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676328 sequences (100000158 bp) in 3.048 CPU sec, 3.287 real sec...
[M::process] read 676280 sequences (100000011 bp) in 2.119 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288152, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.77, 119.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 323, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207)
[M::mem_pestat] mean and std.dev: (226.67, 180.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1600)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676486 reads in 217.492 CPU sec, 6.966 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287863, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (239.99, 121.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (110, 212, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1205)
[M::mem_pestat] mean and std.dev: (269.71, 222.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1570)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676328 reads in 121.015 CPU sec, 3.861 real sec
[M::process] read 676138 sequences (100000065 bp) in 2.436 CPU sec, 2.457 real sec...
sorting block 59, #reads = 1082510 mem = 536880393 peak = 1241497600
[M::mem_process_seqs] Processed 676416 reads in 302.888 CPU sec, 9.680 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288268, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (240.26, 121.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 16, 7491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22453)
[M::mem_pestat] mean and std.dev: (2082.45, 3313.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 29934)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 60, #reads = 1082428 mem = 536881843 peak = 1241497600
[M::process] read 676004 sequences (100000216 bp) in 3.040 CPU sec, 3.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287485, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (240.94, 121.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 426, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2140)
[M::mem_pestat] mean and std.dev: (395.00, 368.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2844)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676280 reads in 235.836 CPU sec, 7.575 real sec
[M::mem_process_seqs] Processed 676138 reads in 131.352 CPU sec, 4.214 real sec
[M::process] read 675852 sequences (100000080 bp) in 2.374 CPU sec, 2.558 real sec...
sorting block 61, #reads = 1081947 mem = 536877161 peak = 1241497600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287690, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.29, 121.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676520 sequences (100000143 bp) in 3.006 CPU sec, 3.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288082, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (243.51, 123.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 116, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1910)
[M::mem_pestat] mean and std.dev: (224.83, 268.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2523)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676004 reads in 222.701 CPU sec, 7.137 real sec
[M::process] read 676926 sequences (100000254 bp) in 2.114 CPU sec, 2.118 real sec...
[M::mem_process_seqs] Processed 675852 reads in 127.236 CPU sec, 4.014 real sec
sorting block 62, #reads = 1081860 mem = 536882505 peak = 1241497600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287772, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.02, 113.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676770 sequences (100000079 bp) in 3.118 CPU sec, 3.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288580, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.67, 110.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 18, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298)
[M::mem_pestat] mean and std.dev: (49.00, 66.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 396)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676734 sequences (100000004 bp) in 2.186 CPU sec, 2.189 real sec...
[M::mem_process_seqs] Processed 676520 reads in 221.009 CPU sec, 7.092 real sec
sorting block 63, #reads = 1081078 mem = 536880453 peak = 1241497600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288104, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.20, 111.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676770 reads in 114.433 CPU sec, 3.650 real sec
[M::process] read 676668 sequences (100000085 bp) in 2.431 CPU sec, 2.480 real sec...
[M::mem_process_seqs] Processed 676926 reads in 295.536 CPU sec, 9.419 real sec
sorting block 64, #reads = 1081705 mem = 536880377 peak = 1241497600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288201, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.11, 110.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676636 sequences (100000025 bp) in 2.997 CPU sec, 3.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288006, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (232.09, 111.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676734 reads in 228.483 CPU sec, 7.288 real sec
[M::mem_process_seqs] Processed 676668 reads in 127.747 CPU sec, 4.077 real sec
[M::process] read 676530 sequences (100000074 bp) in 2.094 CPU sec, 2.197 real sec...
sorting block 65, #reads = 1082425 mem = 536879262 peak = 1241497600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287891, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.55, 113.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 54, 176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (74.38, 63.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 656)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676658 sequences (100000262 bp) in 2.753 CPU sec, 2.958 real sec...
[M::process] read 676476 sequences (100000078 bp) in 2.018 CPU sec, 2.047 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287755, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.15, 114.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676636 reads in 223.717 CPU sec, 7.207 real sec
[M::mem_process_seqs] Processed 676530 reads in 129.794 CPU sec, 4.149 real sec
sorting block 66, #reads = 1082142 mem = 536878464 peak = 1241497600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287482, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.30, 113.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1081515 mem = 536881763 peak = 1241497600
[M::process] read 676498 sequences (100000040 bp) in 3.099 CPU sec, 3.448 real sec...
[M::process] read 676440 sequences (100000000 bp) in 2.138 CPU sec, 2.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287844, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.81, 114.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 21, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175)
[M::mem_pestat] mean and std.dev: (123.47, 177.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1563)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676658 reads in 224.712 CPU sec, 7.247 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287804, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.80, 115.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676368 sequences (100000100 bp) in 2.321 CPU sec, 2.357 real sec...
[M::mem_process_seqs] Processed 676498 reads in 119.723 CPU sec, 3.817 real sec
sorting block 68, #reads = 1081820 mem = 536877970 peak = 1241497600
[M::mem_process_seqs] Processed 676476 reads in 292.686 CPU sec, 9.390 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287988, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.41, 117.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676426 sequences (100000009 bp) in 3.345 CPU sec, 3.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288154, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.00, 117.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676440 reads in 234.372 CPU sec, 7.486 real sec
[M::process] read 676494 sequences (100000102 bp) in 2.115 CPU sec, 2.118 real sec...
sorting block 69, #reads = 1082149 mem = 536880497 peak = 1241497600
[M::mem_process_seqs] Processed 676368 reads in 127.857 CPU sec, 4.048 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287954, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.29, 118.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 89, 89)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253)
[M::mem_pestat] mean and std.dev: (50.00, 36.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 335)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 70, #reads = 1082348 mem = 536878924 peak = 1249443840
[M::process] read 676436 sequences (100000243 bp) in 2.985 CPU sec, 3.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287733, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.86, 115.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676456 sequences (100000031 bp) in 2.161 CPU sec, 2.204 real sec...
[M::mem_process_seqs] Processed 676426 reads in 225.071 CPU sec, 7.289 real sec
[M::mem_process_seqs] Processed 676494 reads in 130.823 CPU sec, 4.206 real sec
sorting block 71, #reads = 1082139 mem = 536880426 peak = 1249443840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287707, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.82, 117.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676600 sequences (100000213 bp) in 3.141 CPU sec, 3.458 real sec...
[M::process] read 676234 sequences (100000083 bp) in 2.120 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287915, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.29, 116.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 453, 576)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1714)
[M::mem_pestat] mean and std.dev: (297.30, 269.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2283)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676436 reads in 222.549 CPU sec, 7.120 real sec
[M::mem_process_seqs] Processed 676456 reads in 127.447 CPU sec, 4.040 real sec
sorting block 72, #reads = 1081955 mem = 536878584 peak = 1249443840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287917, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.48, 117.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676234 sequences (100000299 bp) in 2.876 CPU sec, 3.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287343, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.56, 119.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676200 sequences (100000123 bp) in 2.317 CPU sec, 2.359 real sec...
[M::mem_process_seqs] Processed 676600 reads in 227.946 CPU sec, 7.355 real sec
[M::mem_process_seqs] Processed 676234 reads in 131.799 CPU sec, 4.210 real sec
sorting block 73, #reads = 1082030 mem = 536878728 peak = 1249443840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287779, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.88, 120.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676204 sequences (100000077 bp) in 3.250 CPU sec, 3.914 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287046, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.17, 120.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676260 sequences (100000251 bp) in 2.207 CPU sec, 2.215 real sec...
[M::mem_process_seqs] Processed 676234 reads in 231.636 CPU sec, 7.471 real sec
sorting block 74, #reads = 1082000 mem = 536878996 peak = 1251667968
[M::mem_process_seqs] Processed 676200 reads in 134.316 CPU sec, 4.277 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287902, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.55, 121.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 75, #reads = 1081724 mem = 536881441 peak = 1251667968
[M::process] read 676124 sequences (100000062 bp) in 3.121 CPU sec, 3.400 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287705, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.05, 122.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 23, 23)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 63)
[M::mem_pestat] mean and std.dev: (15.73, 9.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 83)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676116 sequences (100000143 bp) in 2.138 CPU sec, 2.182 real sec...
[M::mem_process_seqs] Processed 676204 reads in 230.530 CPU sec, 7.419 real sec
[M::mem_process_seqs] Processed 676260 reads in 131.795 CPU sec, 4.197 real sec
sorting block 76, #reads = 1082123 mem = 536878680 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287217, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (242.93, 122.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 8, 24)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 56)
[M::mem_pestat] mean and std.dev: (13.00, 7.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 72)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676076 sequences (100000136 bp) in 3.170 CPU sec, 3.423 real sec...
[M::process] read 676078 sequences (100000225 bp) in 2.124 CPU sec, 2.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287263, 18, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.88, 121.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 31, 51)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 117)
[M::mem_pestat] mean and std.dev: (23.87, 16.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 150)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676124 reads in 233.273 CPU sec, 7.501 real sec
[M::mem_process_seqs] Processed 676116 reads in 132.010 CPU sec, 4.230 real sec
sorting block 77, #reads = 1081621 mem = 536878423 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287169, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.46, 122.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676016 sequences (100000146 bp) in 3.082 CPU sec, 3.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287391, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.60, 121.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676146 sequences (100000299 bp) in 2.202 CPU sec, 2.210 real sec...
[M::mem_process_seqs] Processed 676076 reads in 232.472 CPU sec, 7.459 real sec
[M::mem_process_seqs] Processed 676078 reads in 134.117 CPU sec, 4.251 real sec
sorting block 78, #reads = 1080960 mem = 536879235 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287201, 15, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (244.07, 123.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 232, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1688)
[M::mem_pestat] mean and std.dev: (354.27, 275.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2227)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676134 sequences (100000128 bp) in 2.955 CPU sec, 3.500 real sec...
sorting block 79, #reads = 1081567 mem = 536881440 peak = 1251667968
[M::process] read 676080 sequences (100000106 bp) in 2.199 CPU sec, 2.204 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287154, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.41, 123.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (57, 131, 738)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2100)
[M::mem_pestat] mean and std.dev: (359.07, 318.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2781)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676016 reads in 230.090 CPU sec, 7.419 real sec
[M::mem_process_seqs] Processed 676146 reads in 132.544 CPU sec, 4.231 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287041, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.26, 122.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 80, #reads = 1081093 mem = 536878458 peak = 1251667968
[M::process] read 675840 sequences (100000259 bp) in 3.022 CPU sec, 3.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286807, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676146 sequences (100000251 bp) in 2.189 CPU sec, 2.209 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.49, 123.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 7, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095)
[M::mem_pestat] mean and std.dev: (103.92, 162.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1459)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676134 reads in 236.622 CPU sec, 7.640 real sec
[M::mem_process_seqs] Processed 676080 reads in 136.610 CPU sec, 4.331 real sec
sorting block 81, #reads = 1081537 mem = 536879724 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286974, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (243.97, 123.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 86, 3582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10680)
[M::mem_pestat] mean and std.dev: (1557.79, 1753.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14229)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676042 sequences (100000103 bp) in 3.293 CPU sec, 3.511 real sec...
[M::process] read 676380 sequences (100000005 bp) in 2.140 CPU sec, 2.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287024, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.60, 123.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 218, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (152.60, 150.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675840 reads in 231.442 CPU sec, 7.383 real sec
[M::mem_process_seqs] Processed 676146 reads in 134.118 CPU sec, 4.244 real sec
sorting block 82, #reads = 1081781 mem = 536879339 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287593, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.33, 123.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676516 sequences (100000223 bp) in 2.837 CPU sec, 3.276 real sec...
[M::process] read 676360 sequences (100000162 bp) in 2.204 CPU sec, 2.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287863, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (240.12, 121.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 1176, 1347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3951)
[M::mem_pestat] mean and std.dev: (1114.38, 906.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5253)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676042 reads in 231.107 CPU sec, 7.432 real sec
[M::mem_process_seqs] Processed 676380 reads in 132.579 CPU sec, 4.196 real sec
sorting block 83, #reads = 1081778 mem = 536877484 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288028, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.77, 117.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 84, #reads = 1082123 mem = 536878226 peak = 1251667968
[M::process] read 676426 sequences (100000065 bp) in 3.319 CPU sec, 3.892 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287676, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.49, 116.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676516 reads in 226.817 CPU sec, 7.317 real sec
[M::mem_process_seqs] Processed 676360 reads in 132.869 CPU sec, 4.190 real sec
[M::process] read 676372 sequences (100000195 bp) in 2.533 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288255, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.40, 117.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 85, #reads = 1082504 mem = 536877566 peak = 1251667968
[M::process] read 676460 sequences (100000149 bp) in 2.867 CPU sec, 3.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287752, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.53, 117.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676426 reads in 227.329 CPU sec, 7.373 real sec
[M::process] read 676328 sequences (100000108 bp) in 2.193 CPU sec, 2.228 real sec...
[M::mem_process_seqs] Processed 676372 reads in 131.457 CPU sec, 4.254 real sec
sorting block 86, #reads = 1081565 mem = 536882242 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287427, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.58, 117.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 993, 2368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7022)
[M::mem_pestat] mean and std.dev: (991.92, 1027.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9349)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676374 sequences (100000111 bp) in 3.306 CPU sec, 3.638 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287944, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.93, 117.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 97, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1935)
[M::mem_pestat] mean and std.dev: (160.20, 236.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2575)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676228 sequences (100000131 bp) in 2.147 CPU sec, 2.226 real sec...
[M::mem_process_seqs] Processed 676460 reads in 231.821 CPU sec, 7.431 real sec
[M::mem_process_seqs] Processed 676328 reads in 132.456 CPU sec, 4.201 real sec
sorting block 87, #reads = 1081857 mem = 536878102 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287699, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.46, 117.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 39, 63)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 165)
[M::mem_pestat] mean and std.dev: (31.83, 21.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 216)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676420 sequences (100000213 bp) in 3.204 CPU sec, 3.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287388, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.64, 119.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 40, 1288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3816)
[M::mem_pestat] mean and std.dev: (331.15, 526.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5080)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676322 sequences (100000106 bp) in 2.225 CPU sec, 2.236 real sec...
[M::mem_process_seqs] Processed 676374 reads in 230.409 CPU sec, 7.376 real sec
[M::mem_process_seqs] Processed 676228 reads in 132.207 CPU sec, 4.175 real sec
sorting block 88, #reads = 1082423 mem = 536878847 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287931, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.89, 119.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 89, #reads = 1082139 mem = 536878664 peak = 1251667968
[M::process] read 676252 sequences (100000271 bp) in 3.204 CPU sec, 3.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287223, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.51, 119.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676294 sequences (100000219 bp) in 2.200 CPU sec, 2.208 real sec...
[M::mem_process_seqs] Processed 676420 reads in 230.420 CPU sec, 7.419 real sec
[M::mem_process_seqs] Processed 676322 reads in 133.324 CPU sec, 4.245 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287417, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.96, 119.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 90, #reads = 1082312 mem = 536879693 peak = 1251667968
[M::process] read 676498 sequences (100000214 bp) in 2.861 CPU sec, 3.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287928, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676172 sequences (100000030 bp) in 2.237 CPU sec, 2.288 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.59, 119.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676252 reads in 232.042 CPU sec, 7.485 real sec
[M::mem_process_seqs] Processed 676294 reads in 132.940 CPU sec, 4.221 real sec
sorting block 91, #reads = 1081841 mem = 536878684 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288302, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.09, 120.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675998 sequences (100000177 bp) in 3.136 CPU sec, 3.682 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287489, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.14, 121.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 71, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (89.75, 84.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675928 sequences (100000069 bp) in 2.237 CPU sec, 2.245 real sec...
[M::mem_process_seqs] Processed 676498 reads in 224.705 CPU sec, 7.240 real sec
[M::mem_process_seqs] Processed 676172 reads in 130.814 CPU sec, 4.164 real sec
sorting block 92, #reads = 1082335 mem = 536879326 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287414, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.63, 122.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676120 sequences (100000147 bp) in 2.867 CPU sec, 3.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287722, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.61, 121.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 102, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (65.70, 74.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676970 sequences (100000195 bp) in 2.316 CPU sec, 2.321 real sec...
[M::mem_process_seqs] Processed 675998 reads in 224.579 CPU sec, 7.222 real sec
[M::mem_process_seqs] Processed 675928 reads in 127.522 CPU sec, 4.032 real sec
sorting block 93, #reads = 1081547 mem = 536877220 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288071, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (239.13, 118.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 75, 668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1974)
[M::mem_pestat] mean and std.dev: (446.70, 685.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3190)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 94, #reads = 1081331 mem = 536877594 peak = 1251667968
[M::process] read 676762 sequences (100000032 bp) in 3.625 CPU sec, 3.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288177, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.77, 108.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676120 reads in 221.015 CPU sec, 7.090 real sec
[M::process] read 676822 sequences (100000155 bp) in 2.271 CPU sec, 2.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288033, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.35, 109.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676762 reads in 117.779 CPU sec, 3.790 real sec
[M::process] read 676746 sequences (100000097 bp) in 2.773 CPU sec, 2.869 real sec...
sorting block 95, #reads = 1081900 mem = 536876629 peak = 1251667968
[M::mem_process_seqs] Processed 676970 reads in 318.430 CPU sec, 10.230 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287976, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.22, 110.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 610, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2075)
[M::mem_pestat] mean and std.dev: (359.73, 324.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2763)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676712 sequences (100000129 bp) in 2.842 CPU sec, 3.495 real sec...
sorting block 96, #reads = 1082413 mem = 536880213 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287355, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.19, 110.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 547, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1597)
[M::mem_pestat] mean and std.dev: (488.60, 403.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2103)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676822 reads in 241.320 CPU sec, 7.775 real sec
[M::mem_process_seqs] Processed 676746 reads in 133.975 CPU sec, 4.303 real sec
[M::process] read 676474 sequences (100000128 bp) in 2.638 CPU sec, 2.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287812, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (232.27, 111.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 33, 33)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 91)
[M::mem_pestat] mean and std.dev: (25.80, 19.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 120)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 97, #reads = 1082062 mem = 536880165 peak = 1251667968
[M::process] read 676602 sequences (100000115 bp) in 3.037 CPU sec, 3.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287918, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (232.14, 111.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676712 reads in 227.140 CPU sec, 7.323 real sec
[M::process] read 676682 sequences (100000176 bp) in 2.117 CPU sec, 2.262 real sec...
[M::mem_process_seqs] Processed 676474 reads in 130.521 CPU sec, 4.186 real sec
sorting block 98, #reads = 1082144 mem = 536879081 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287633, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (233.28, 112.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 89, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575)
[M::mem_pestat] mean and std.dev: (111.54, 81.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 737)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676616 sequences (100000115 bp) in 3.259 CPU sec, 3.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287886, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676472 sequences (100000144 bp) in 2.206 CPU sec, 2.216 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.94, 113.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676602 reads in 227.486 CPU sec, 7.350 real sec
[M::mem_process_seqs] Processed 676682 reads in 130.314 CPU sec, 4.225 real sec
sorting block 99, #reads = 1082029 mem = 536877059 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287746, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.23, 113.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676382 sequences (100000036 bp) in 2.983 CPU sec, 3.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287514, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.52, 112.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676450 sequences (100000290 bp) in 2.181 CPU sec, 2.184 real sec...
[M::mem_process_seqs] Processed 676616 reads in 228.480 CPU sec, 7.326 real sec
[M::mem_process_seqs] Processed 676472 reads in 132.868 CPU sec, 4.204 real sec
sorting block 100, #reads = 1082386 mem = 536878618 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287345, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.80, 113.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 101, #reads = 1081842 mem = 536879650 peak = 1251667968
[M::process] read 676314 sequences (100000204 bp) in 3.436 CPU sec, 3.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287961, 14, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (236.26, 115.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 176, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (194.07, 170.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676504 sequences (100000252 bp) in 2.131 CPU sec, 2.168 real sec...
[M::mem_process_seqs] Processed 676382 reads in 233.919 CPU sec, 7.569 real sec
[M::mem_process_seqs] Processed 676450 reads in 134.094 CPU sec, 4.247 real sec
sorting block 102, #reads = 1081630 mem = 536875142 peak = 1251667968
[M::process] read 676320 sequences (100000280 bp) in 3.086 CPU sec, 3.386 real sec...
[M::process] read 676484 sequences (100000267 bp) in 2.124 CPU sec, 2.132 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287768, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.93, 115.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 9, 58)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 160)
[M::mem_pestat] mean and std.dev: (19.53, 21.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 211)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676504 reads in 123.728 CPU sec, 3.895 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287568, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.89, 117.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 423, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (312.36, 171.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288284, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.24, 116.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676314 reads in 351.711 CPU sec, 11.268 real sec
[M::mem_process_seqs] Processed 676320 reads in 142.303 CPU sec, 4.554 real sec
[M::process] read 676232 sequences (100000088 bp) in 2.337 CPU sec, 2.364 real sec...
sorting block 103, #reads = 1082562 mem = 536879461 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287705, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (236.19, 116.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 104, #reads = 1082210 mem = 536876811 peak = 1251667968
[M::process] read 676460 sequences (100000283 bp) in 3.008 CPU sec, 3.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287459, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.25, 117.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 45, 79)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177)
[M::mem_pestat] mean and std.dev: (35.44, 23.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 226)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676484 reads in 226.221 CPU sec, 7.329 real sec
[M::process] read 676240 sequences (100000091 bp) in 2.275 CPU sec, 2.380 real sec...
[M::mem_process_seqs] Processed 676232 reads in 132.925 CPU sec, 4.217 real sec
sorting block 105, #reads = 1081958 mem = 536878187 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287583, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.46, 119.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676096 sequences (100000086 bp) in 2.868 CPU sec, 3.321 real sec...
[M::process] read 676150 sequences (100000279 bp) in 2.136 CPU sec, 2.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287307, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.87, 118.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676460 reads in 231.234 CPU sec, 7.446 real sec
[M::mem_process_seqs] Processed 676240 reads in 131.321 CPU sec, 4.190 real sec
sorting block 106, #reads = 1082062 mem = 536879542 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287522, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.81, 119.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676038 sequences (100000292 bp) in 3.121 CPU sec, 3.668 real sec...
[M::process] read 676110 sequences (100000089 bp) in 2.262 CPU sec, 2.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287382, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.98, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676096 reads in 234.273 CPU sec, 7.537 real sec
[M::mem_process_seqs] Processed 676150 reads in 133.976 CPU sec, 4.258 real sec
sorting block 107, #reads = 1081643 mem = 536878265 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287009, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (240.46, 119.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 119, 867)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2559)
[M::mem_pestat] mean and std.dev: (312.80, 373.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3405)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676170 sequences (100000162 bp) in 2.623 CPU sec, 2.854 real sec...
[M::process] read 676098 sequences (100000189 bp) in 2.015 CPU sec, 2.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286821, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.83, 120.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676038 reads in 237.908 CPU sec, 7.674 real sec
sorting block 108, #reads = 1081977 mem = 536878248 peak = 1251667968
[M::mem_process_seqs] Processed 676110 reads in 136.403 CPU sec, 4.358 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287155, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (241.34, 120.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 109, #reads = 1081567 mem = 536881232 peak = 1251667968
[M::process] read 676088 sequences (100000094 bp) in 3.349 CPU sec, 3.587 real sec...
[M::process] read 676040 sequences (100000278 bp) in 2.150 CPU sec, 2.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287162, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.60, 120.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676170 reads in 233.544 CPU sec, 7.492 real sec
[M::mem_process_seqs] Processed 676098 reads in 135.574 CPU sec, 4.326 real sec
sorting block 110, #reads = 1081384 mem = 536880348 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287342, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.58, 120.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676078 sequences (100000286 bp) in 2.994 CPU sec, 3.385 real sec...
[M::process] read 675936 sequences (100000172 bp) in 2.135 CPU sec, 2.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287341, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (240.74, 119.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676088 reads in 233.321 CPU sec, 7.524 real sec
[M::mem_process_seqs] Processed 676040 reads in 133.807 CPU sec, 4.261 real sec
sorting block 111, #reads = 1082086 mem = 536878581 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287512, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.59, 120.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676028 sequences (100000259 bp) in 2.881 CPU sec, 3.182 real sec...
[M::process] read 676080 sequences (100000071 bp) in 2.216 CPU sec, 2.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286956, 20, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.55, 121.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 196, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1649)
[M::mem_pestat] mean and std.dev: (276.45, 251.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2186)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676078 reads in 234.568 CPU sec, 7.522 real sec
[M::mem_process_seqs] Processed 675936 reads in 134.287 CPU sec, 4.255 real sec
sorting block 112, #reads = 1081649 mem = 536878583 peak = 1251667968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287315, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.83, 122.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 113, #reads = 1081384 mem = 536877489 peak = 1260101632
[M::process] read 676024 sequences (100000259 bp) in 2.904 CPU sec, 3.175 real sec...
[M::process] read 676090 sequences (100000106 bp) in 2.230 CPU sec, 2.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287362, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.15, 120.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676028 reads in 235.018 CPU sec, 7.600 real sec
[M::mem_process_seqs] Processed 676080 reads in 134.546 CPU sec, 4.326 real sec
sorting block 114, #reads = 1081753 mem = 536879460 peak = 1260101632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287446, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.56, 120.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 10, 1187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3551)
[M::mem_pestat] mean and std.dev: (353.55, 516.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4733)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676400 sequences (100000060 bp) in 3.099 CPU sec, 3.609 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287153, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676444 sequences (100000163 bp) in 2.183 CPU sec, 2.190 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (241.08, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676024 reads in 231.692 CPU sec, 7.456 real sec
[M::mem_process_seqs] Processed 676090 reads in 131.674 CPU sec, 4.181 real sec
sorting block 115, #reads = 1081450 mem = 536878450 peak = 1260101632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287637, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (236.42, 116.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676522 sequences (100000053 bp) in 2.569 CPU sec, 2.858 real sec...
[M::process] read 676420 sequences (100000195 bp) in 1.963 CPU sec, 1.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288141, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.95, 115.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 39, 826)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2410)
[M::mem_pestat] mean and std.dev: (258.78, 336.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3202)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676400 reads in 230.561 CPU sec, 7.465 real sec
[M::mem_process_seqs] Processed 676444 reads in 130.399 CPU sec, 4.172 real sec
sorting block 116, #reads = 1082227 mem = 536880263 peak = 1260101632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287624, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.52, 115.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676382 sequences (100000264 bp) in 3.347 CPU sec, 3.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287423, 24, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.67, 114.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (262, 269, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 637)
[M::mem_pestat] mean and std.dev: (259.90, 83.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676352 sequences (100000160 bp) in 2.151 CPU sec, 2.189 real sec...
[M::mem_process_seqs] Processed 676522 reads in 228.190 CPU sec, 7.309 real sec
[M::mem_process_seqs] Processed 676420 reads in 129.808 CPU sec, 4.093 real sec
sorting block 117, #reads = 1082514 mem = 536878693 peak = 1260101632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287562, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.90, 115.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 34, 75)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 189)
[M::mem_pestat] mean and std.dev: (39.11, 26.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 246)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 118, #reads = 1082083 mem = 536877475 peak = 1269542912
[M::process] read 676360 sequences (100000248 bp) in 2.894 CPU sec, 3.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288306, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.96, 116.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676424 sequences (100000157 bp) in 2.218 CPU sec, 2.229 real sec...
[M::mem_process_seqs] Processed 676382 reads in 228.288 CPU sec, 7.378 real sec
[M::mem_process_seqs] Processed 676352 reads in 131.534 CPU sec, 4.179 real sec
sorting block 119, #reads = 1082148 mem = 536880406 peak = 1269542912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287900, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (235.15, 115.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 71, 793)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2303)
[M::mem_pestat] mean and std.dev: (374.23, 372.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3058)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676354 sequences (100000186 bp) in 3.184 CPU sec, 3.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287910, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.87, 115.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 880, 880)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2598)
[M::mem_pestat] mean and std.dev: (679.67, 771.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3764)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676360 reads in 225.703 CPU sec, 7.259 real sec
[M::process] read 676230 sequences (100000173 bp) in 2.278 CPU sec, 2.283 real sec...
[M::mem_process_seqs] Processed 676424 reads in 128.778 CPU sec, 4.090 real sec
sorting block 120, #reads = 1082221 mem = 536880730 peak = 1269542912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287932, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.64, 117.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676240 sequences (100000151 bp) in 2.718 CPU sec, 3.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287836, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.23, 118.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (100, 658, 734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2002)
[M::mem_pestat] mean and std.dev: (402.92, 320.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2636)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676200 sequences (100000147 bp) in 2.113 CPU sec, 2.126 real sec...
[M::mem_process_seqs] Processed 676354 reads in 227.264 CPU sec, 7.372 real sec
[M::mem_process_seqs] Processed 676230 reads in 130.072 CPU sec, 4.166 real sec
sorting block 121, #reads = 1082205 mem = 536879664 peak = 1269542912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287984, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.50, 118.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676206 sequences (100000162 bp) in 2.917 CPU sec, 3.084 real sec...
[M::process] read 676222 sequences (100000177 bp) in 2.249 CPU sec, 2.268 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287812, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (238.17, 118.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676240 reads in 224.669 CPU sec, 7.188 real sec
[M::mem_process_seqs] Processed 676200 reads in 129.019 CPU sec, 4.082 real sec
sorting block 122, #reads = 1082302 mem = 536878496 peak = 1269542912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287865, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.09, 119.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 123, #reads = 1082682 mem = 536879693 peak = 1269542912
[M::process] read 676076 sequences (100000109 bp) in 3.037 CPU sec, 3.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287705, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (238.64, 118.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676206 reads in 223.792 CPU sec, 7.256 real sec
[M::process] read 675892 sequences (100000201 bp) in 2.240 CPU sec, 2.459 real sec...
[M::mem_process_seqs] Processed 676222 reads in 128.743 CPU sec, 4.191 real sec
sorting block 124, #reads = 1081839 mem = 536877775 peak = 1269542912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287187, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.42, 121.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 89, 1519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4379)
[M::mem_pestat] mean and std.dev: (538.64, 639.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5809)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675998 sequences (100000268 bp) in 3.324 CPU sec, 3.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287668, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.50, 122.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676076 reads in 222.226 CPU sec, 7.141 real sec
[M::process] read 676302 sequences (100000032 bp) in 2.085 CPU sec, 2.095 real sec...
[M::mem_process_seqs] Processed 675892 reads in 127.568 CPU sec, 4.039 real sec
sorting block 125, #reads = 1081598 mem = 536880075 peak = 1269542912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287929, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.56, 120.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677366 sequences (100000165 bp) in 2.817 CPU sec, 3.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287739, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.21, 119.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 86, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (79.45, 64.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677068 sequences (100000166 bp) in 2.214 CPU sec, 2.217 real sec...
[M::mem_process_seqs] Processed 675998 reads in 219.614 CPU sec, 7.070 real sec
[M::mem_process_seqs] Processed 676302 reads in 129.127 CPU sec, 4.102 real sec
sorting block 126, #reads = 1081744 mem = 536879098 peak = 1269542912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287647, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (224.95, 107.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 72, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1849)
[M::mem_pestat] mean and std.dev: (299.10, 419.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2455)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677000 sequences (100000071 bp) in 3.279 CPU sec, 3.760 real sec...
[M::process] read 676984 sequences (100000114 bp) in 2.240 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287834, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.22, 110.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 10, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (90.27, 164.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677366 reads in 237.044 CPU sec, 7.648 real sec
[M::mem_process_seqs] Processed 677068 reads in 134.608 CPU sec, 4.274 real sec
sorting block 127, #reads = 1081447 mem = 536878137 peak = 1269542912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287612, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.08, 110.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 128, #reads = 1082059 mem = 536879861 peak = 1279000576
[M::process] read 676846 sequences (100000242 bp) in 2.736 CPU sec, 3.074 real sec...
[M::process] read 676818 sequences (100000083 bp) in 2.266 CPU sec, 2.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287459, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.17, 112.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677000 reads in 236.261 CPU sec, 7.630 real sec
[M::mem_process_seqs] Processed 676984 reads in 136.642 CPU sec, 4.370 real sec
sorting block 129, #reads = 1082437 mem = 536881339 peak = 1279000576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287192, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.78, 112.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676852 sequences (100000143 bp) in 3.707 CPU sec, 3.950 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287486, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.76, 114.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676760 sequences (100000271 bp) in 2.246 CPU sec, 2.269 real sec...
[M::mem_process_seqs] Processed 676846 reads in 238.439 CPU sec, 7.611 real sec
[M::mem_process_seqs] Processed 676818 reads in 137.773 CPU sec, 4.358 real sec
sorting block 130, #reads = 1082144 mem = 536878679 peak = 1279000576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288103, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.01, 113.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 195, 574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1668)
[M::mem_pestat] mean and std.dev: (247.33, 238.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2215)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676770 sequences (100000121 bp) in 3.261 CPU sec, 3.545 real sec...
[M::process] read 676736 sequences (100000233 bp) in 2.234 CPU sec, 2.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287464, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.93, 113.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 181, 190)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (107.50, 113.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676852 reads in 235.598 CPU sec, 7.556 real sec
[M::mem_process_seqs] Processed 676760 reads in 135.403 CPU sec, 4.294 real sec
sorting block 131, #reads = 1082339 mem = 536879613 peak = 1279000576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287961, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.47, 113.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676692 sequences (100000208 bp) in 3.395 CPU sec, 3.641 real sec...
[M::process] read 676668 sequences (100000129 bp) in 2.240 CPU sec, 2.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287513, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.73, 113.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676770 reads in 237.846 CPU sec, 7.615 real sec
sorting block 132, #reads = 1081977 mem = 536877459 peak = 1279000576
[M::mem_process_seqs] Processed 676736 reads in 136.488 CPU sec, 4.333 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287663, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.06, 114.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 133, #reads = 1082017 mem = 536879421 peak = 1279000576
[M::process] read 676760 sequences (100000229 bp) in 2.843 CPU sec, 3.129 real sec...
[M::process] read 676904 sequences (100000029 bp) in 2.249 CPU sec, 2.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287152, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.15, 114.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676692 reads in 238.444 CPU sec, 7.736 real sec
[M::mem_process_seqs] Processed 676668 reads in 135.720 CPU sec, 4.334 real sec
sorting block 134, #reads = 1082480 mem = 536878150 peak = 1279000576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287681, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.42, 112.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676790 sequences (100000294 bp) in 3.199 CPU sec, 3.594 real sec...
[M::process] read 676718 sequences (100000259 bp) in 2.250 CPU sec, 2.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287794, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.86, 112.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (247, 339, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 592)
[M::mem_pestat] mean and std.dev: (284.20, 98.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 707)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676760 reads in 235.710 CPU sec, 7.619 real sec
[M::mem_process_seqs] Processed 676904 reads in 132.736 CPU sec, 4.241 real sec
sorting block 135, #reads = 1081683 mem = 536879081 peak = 1279000576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287737, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.19, 113.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676610 sequences (100000223 bp) in 2.754 CPU sec, 3.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287375, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676486 sequences (100000099 bp) in 2.223 CPU sec, 2.323 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.89, 114.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 89, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (193.18, 223.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676790 reads in 233.965 CPU sec, 7.590 real sec
[M::mem_process_seqs] Processed 676718 reads in 135.097 CPU sec, 4.321 real sec
sorting block 136, #reads = 1082486 mem = 536878710 peak = 1279000576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287317, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.43, 115.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 76, 76)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184)
[M::mem_pestat] mean and std.dev: (42.00, 31.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 238)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676376 sequences (100000297 bp) in 3.230 CPU sec, 3.789 real sec...
[M::process] read 676300 sequences (100000187 bp) in 2.232 CPU sec, 2.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287678, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.55, 115.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676610 reads in 239.979 CPU sec, 7.714 real sec
sorting block 137, #reads = 1081642 mem = 536878085 peak = 1279000576
[M::mem_process_seqs] Processed 676486 reads in 146.474 CPU sec, 4.636 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287247, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (236.11, 117.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 138, #reads = 1079942 mem = 536880353 peak = 1279000576
[M::process] read 676384 sequences (100000033 bp) in 3.066 CPU sec, 3.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286563, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.31, 118.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676376 reads in 225.879 CPU sec, 7.272 real sec
[M::process] read 676118 sequences (100000000 bp) in 2.362 CPU sec, 2.368 real sec...
[M::mem_process_seqs] Processed 676300 reads in 137.225 CPU sec, 4.373 real sec
sorting block 139, #reads = 1081494 mem = 536878366 peak = 1279000576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287656, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.39, 119.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 107, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1667)
[M::mem_pestat] mean and std.dev: (188.64, 229.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2187)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676306 sequences (100000226 bp) in 3.436 CPU sec, 3.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287732, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.71, 119.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676168 sequences (100000232 bp) in 2.247 CPU sec, 2.250 real sec...
[M::mem_process_seqs] Processed 676384 reads in 238.096 CPU sec, 7.649 real sec
[M::mem_process_seqs] Processed 676118 reads in 137.679 CPU sec, 4.375 real sec
sorting block 140, #reads = 1081473 mem = 536881985 peak = 1279000576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287580, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.72, 119.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676222 sequences (100000252 bp) in 2.779 CPU sec, 2.981 real sec...
[M::process] read 676242 sequences (100000075 bp) in 2.217 CPU sec, 2.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286388, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.33, 120.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676306 reads in 235.772 CPU sec, 7.577 real sec
[M::mem_process_seqs] Processed 676168 reads in 131.045 CPU sec, 4.158 real sec
sorting block 141, #reads = 1082011 mem = 536878826 peak = 1279000576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286998, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.66, 121.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676262 sequences (100000227 bp) in 3.490 CPU sec, 3.824 real sec...
sorting block 142, #reads = 1080863 mem = 536877981 peak = 1279000576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287125, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (240.26, 121.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676408 sequences (100000066 bp) in 2.211 CPU sec, 2.218 real sec...
[M::mem_process_seqs] Processed 676222 reads in 225.552 CPU sec, 7.265 real sec
[M::mem_process_seqs] Processed 676242 reads in 131.972 CPU sec, 4.212 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287140, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.75, 120.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 143, #reads = 1080052 mem = 536881732 peak = 1279000576
[M::process] read 676226 sequences (100000125 bp) in 3.029 CPU sec, 3.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286949, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.63, 121.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676186 sequences (100000179 bp) in 2.316 CPU sec, 2.322 real sec...
[M::mem_process_seqs] Processed 676262 reads in 237.479 CPU sec, 7.699 real sec
[M::mem_process_seqs] Processed 676408 reads in 140.514 CPU sec, 4.512 real sec
sorting block 144, #reads = 1081141 mem = 536880479 peak = 1279000576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287029, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.62, 120.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 18, 140)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414)
[M::mem_pestat] mean and std.dev: (26.33, 41.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 551)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676254 sequences (100000200 bp) in 3.129 CPU sec, 3.528 real sec...
[M::process] read 676178 sequences (100000283 bp) in 1.962 CPU sec, 1.966 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286542, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (240.16, 122.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676226 reads in 255.007 CPU sec, 8.204 real sec
[M::mem_process_seqs] Processed 676186 reads in 143.126 CPU sec, 4.567 real sec
sorting block 145, #reads = 1080862 mem = 536880027 peak = 1279000576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287331, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (241.26, 123.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676152 sequences (100000215 bp) in 2.882 CPU sec, 3.398 real sec...
[M::process] read 676230 sequences (100000158 bp) in 2.175 CPU sec, 2.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286788, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (241.86, 124.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676254 reads in 239.912 CPU sec, 7.771 real sec
[M::mem_process_seqs] Processed 676178 reads in 138.013 CPU sec, 4.460 real sec
sorting block 146, #reads = 1081683 mem = 536877762 peak = 1279000576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287243, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (241.70, 123.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 147, #reads = 1081704 mem = 536880253 peak = 1281236992
[M::process] read 676408 sequences (100000118 bp) in 3.314 CPU sec, 3.593 real sec...
[M::process] read 676524 sequences (100000109 bp) in 2.258 CPU sec, 2.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286758, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.14, 121.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676152 reads in 253.333 CPU sec, 8.213 real sec
[M::mem_process_seqs] Processed 676230 reads in 137.258 CPU sec, 4.440 real sec
sorting block 148, #reads = 1080216 mem = 536877956 peak = 1281236992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287689, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.32, 114.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 209, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1855)
[M::mem_pestat] mean and std.dev: (275.74, 269.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2464)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676658 sequences (100000081 bp) in 2.976 CPU sec, 3.114 real sec...
[M::process] read 676664 sequences (100000124 bp) in 2.343 CPU sec, 2.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287539, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.70, 114.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 28, 28)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 64)
[M::mem_pestat] mean and std.dev: (17.44, 9.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 82)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676408 reads in 237.032 CPU sec, 7.631 real sec
[M::mem_process_seqs] Processed 676524 reads in 135.707 CPU sec, 4.308 real sec
sorting block 149, #reads = 1082194 mem = 536879360 peak = 1281236992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287355, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.15, 114.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676532 sequences (100000054 bp) in 2.995 CPU sec, 3.502 real sec...
[M::process] read 676578 sequences (100000196 bp) in 2.274 CPU sec, 2.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287743, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.93, 115.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676658 reads in 237.442 CPU sec, 7.668 real sec
[M::mem_process_seqs] Processed 676664 reads in 134.897 CPU sec, 4.310 real sec
sorting block 150, #reads = 1082376 mem = 536880829 peak = 1281236992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287015, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.09, 113.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676640 sequences (100000030 bp) in 3.130 CPU sec, 3.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287497, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.44, 114.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676532 reads in 237.745 CPU sec, 7.707 real sec
[M::process] read 676788 sequences (100000013 bp) in 2.382 CPU sec, 2.460 real sec...
[M::mem_process_seqs] Processed 676578 reads in 136.204 CPU sec, 4.343 real sec
sorting block 151, #reads = 1082034 mem = 536881154 peak = 1281236992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287707, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.96, 115.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 152, #reads = 1081497 mem = 536884717 peak = 1281236992
[M::process] read 676794 sequences (100000056 bp) in 3.292 CPU sec, 3.583 real sec...
[M::process] read 676844 sequences (100000263 bp) in 2.059 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287570, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.15, 112.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676640 reads in 235.644 CPU sec, 7.568 real sec
[M::mem_process_seqs] Processed 676788 reads in 133.146 CPU sec, 4.237 real sec
sorting block 153, #reads = 1082430 mem = 536880755 peak = 1281236992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288211, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.67, 112.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676588 sequences (100000071 bp) in 3.221 CPU sec, 3.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287825, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.84, 113.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676666 sequences (100000281 bp) in 2.390 CPU sec, 2.427 real sec...
[M::mem_process_seqs] Processed 676794 reads in 233.817 CPU sec, 7.475 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287685, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.24, 113.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 169, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1508)
[M::mem_pestat] mean and std.dev: (254.90, 235.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1998)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676588 reads in 119.647 CPU sec, 3.850 real sec
sorting block 154, #reads = 1082742 mem = 536880377 peak = 1281236992
[M::process] read 676582 sequences (100000263 bp) in 3.088 CPU sec, 3.151 real sec...
[M::mem_process_seqs] Processed 676844 reads in 327.559 CPU sec, 10.480 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287436, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.20, 115.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 155, #reads = 1081552 mem = 536879267 peak = 1286389760
[M::process] read 676480 sequences (100000244 bp) in 3.147 CPU sec, 3.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287282, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.75, 117.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676666 reads in 247.006 CPU sec, 7.918 real sec
[M::mem_process_seqs] Processed 676582 reads in 137.126 CPU sec, 4.398 real sec
[M::process] read 676572 sequences (100000209 bp) in 2.802 CPU sec, 2.966 real sec...
sorting block 156, #reads = 1082209 mem = 536880907 peak = 1286389760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288071, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.66, 118.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676954 sequences (100000251 bp) in 3.320 CPU sec, 3.721 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287413, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.26, 117.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676480 reads in 232.478 CPU sec, 7.481 real sec
[M::mem_process_seqs] Processed 676572 reads in 135.198 CPU sec, 4.315 real sec
[M::process] read 677238 sequences (100000105 bp) in 2.637 CPU sec, 2.684 real sec...
sorting block 157, #reads = 1082039 mem = 536880318 peak = 1286389760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287920, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.36, 114.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677172 sequences (100000086 bp) in 3.100 CPU sec, 3.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287394, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.25, 108.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676954 reads in 233.250 CPU sec, 7.561 real sec
[M::mem_process_seqs] Processed 677238 reads in 134.081 CPU sec, 4.288 real sec
[M::process] read 677084 sequences (100000173 bp) in 2.491 CPU sec, 2.571 real sec...
sorting block 158, #reads = 1081376 mem = 536877385 peak = 1286389760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288152, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (228.70, 111.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 159, #reads = 1082079 mem = 536877998 peak = 1286389760
[M::process] read 676640 sequences (100000008 bp) in 3.098 CPU sec, 3.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287925, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.26, 112.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677172 reads in 229.106 CPU sec, 7.381 real sec
[M::process] read 676772 sequences (100000176 bp) in 2.242 CPU sec, 2.244 real sec...
[M::mem_process_seqs] Processed 677084 reads in 134.136 CPU sec, 4.263 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287512, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.49, 112.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 160, #reads = 1082021 mem = 536879062 peak = 1286389760
[M::process] read 676900 sequences (100000025 bp) in 3.082 CPU sec, 3.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288103, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.35, 113.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 377, 2612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7756)
[M::mem_pestat] mean and std.dev: (870.18, 1088.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10328)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676640 reads in 207.126 CPU sec, 6.734 real sec
[M::mem_process_seqs] Processed 676772 reads in 110.536 CPU sec, 3.570 real sec
[M::process] read 676728 sequences (100000216 bp) in 2.545 CPU sec, 2.646 real sec...
sorting block 161, #reads = 1080784 mem = 536878330 peak = 1286389760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287709, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.88, 113.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676874 sequences (100000179 bp) in 3.499 CPU sec, 3.726 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287551, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.45, 113.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 79, 571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1643)
[M::mem_pestat] mean and std.dev: (255.36, 254.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2179)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676900 reads in 231.773 CPU sec, 7.434 real sec
[M::process] read 676684 sequences (100000285 bp) in 1.964 CPU sec, 1.986 real sec...
[M::mem_process_seqs] Processed 676728 reads in 130.740 CPU sec, 4.130 real sec
sorting block 162, #reads = 1080561 mem = 536881988 peak = 1286389760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287925, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.65, 114.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676632 sequences (100000298 bp) in 2.887 CPU sec, 3.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287615, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.45, 114.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676694 sequences (100000016 bp) in 2.177 CPU sec, 2.185 real sec...
[M::mem_process_seqs] Processed 676874 reads in 230.886 CPU sec, 7.439 real sec
[M::mem_process_seqs] Processed 676684 reads in 133.632 CPU sec, 4.260 real sec
sorting block 163, #reads = 1081895 mem = 536879525 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287497, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.09, 115.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 164, #reads = 1081630 mem = 536881051 peak = 1287417856
[M::process] read 676810 sequences (100000024 bp) in 3.246 CPU sec, 3.596 real sec...
[M::process] read 676668 sequences (100000209 bp) in 2.002 CPU sec, 2.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287436, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.35, 115.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676632 reads in 227.006 CPU sec, 7.351 real sec
[M::mem_process_seqs] Processed 676694 reads in 129.773 CPU sec, 4.175 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286978, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.43, 116.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 165, #reads = 1079586 mem = 536880262 peak = 1287417856
[M::process] read 676794 sequences (100000185 bp) in 3.091 CPU sec, 3.263 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287316, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.07, 113.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676810 reads in 223.249 CPU sec, 7.153 real sec
[M::mem_process_seqs] Processed 676668 reads in 140.237 CPU sec, 4.440 real sec
[M::process] read 676828 sequences (100000277 bp) in 2.555 CPU sec, 2.660 real sec...
sorting block 166, #reads = 1078632 mem = 536878451 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287835, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.84, 113.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (59, 448, 632)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1778)
[M::mem_pestat] mean and std.dev: (363.67, 258.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2351)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676670 sequences (100000169 bp) in 2.888 CPU sec, 3.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287598, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.69, 114.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676794 reads in 212.319 CPU sec, 6.880 real sec
[M::mem_process_seqs] Processed 676828 reads in 128.631 CPU sec, 4.058 real sec
[M::process] read 676524 sequences (100000118 bp) in 2.240 CPU sec, 2.263 real sec...
sorting block 167, #reads = 1080087 mem = 536880073 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287509, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.26, 114.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676642 sequences (100000102 bp) in 2.810 CPU sec, 3.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287640, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.66, 113.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 880, 980)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2490)
[M::mem_pestat] mean and std.dev: (624.60, 420.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3245)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676404 sequences (100000022 bp) in 2.236 CPU sec, 2.244 real sec...
[M::mem_process_seqs] Processed 676670 reads in 234.454 CPU sec, 7.567 real sec
[M::mem_process_seqs] Processed 676524 reads in 132.950 CPU sec, 4.233 real sec
sorting block 168, #reads = 1077733 mem = 536879786 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287954, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.47, 115.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 169, #reads = 1081647 mem = 536879832 peak = 1287417856
[M::process] read 676366 sequences (100000194 bp) in 3.271 CPU sec, 3.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287946, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.62, 117.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676642 reads in 224.482 CPU sec, 7.263 real sec
[M::process] read 676238 sequences (100000095 bp) in 2.369 CPU sec, 2.406 real sec...
[M::mem_process_seqs] Processed 676404 reads in 128.255 CPU sec, 4.050 real sec
sorting block 170, #reads = 1077685 mem = 536878382 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287781, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.80, 120.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676354 sequences (100000055 bp) in 2.889 CPU sec, 3.294 real sec...
[M::process] read 676388 sequences (100000255 bp) in 2.249 CPU sec, 2.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287489, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (240.25, 121.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676366 reads in 235.290 CPU sec, 7.542 real sec
[M::mem_process_seqs] Processed 676238 reads in 133.130 CPU sec, 4.234 real sec
sorting block 171, #reads = 1078931 mem = 536880124 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287775, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.78, 120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676394 sequences (100000096 bp) in 3.234 CPU sec, 3.475 real sec...
[M::process] read 676216 sequences (100000171 bp) in 2.196 CPU sec, 2.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287287, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.35, 120.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676354 reads in 232.005 CPU sec, 7.429 real sec
[M::mem_process_seqs] Processed 676388 reads in 134.398 CPU sec, 4.286 real sec
sorting block 172, #reads = 1082076 mem = 536878960 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287178, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.21, 121.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676296 sequences (100000210 bp) in 2.890 CPU sec, 3.270 real sec...
[M::process] read 676344 sequences (100000273 bp) in 2.431 CPU sec, 2.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287254, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.88, 122.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676394 reads in 237.819 CPU sec, 7.635 real sec
sorting block 173, #reads = 1082282 mem = 536879616 peak = 1287417856
[M::mem_process_seqs] Processed 676216 reads in 135.642 CPU sec, 4.296 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287627, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (240.30, 122.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 174, #reads = 1081762 mem = 536879691 peak = 1287417856
[M::process] read 676270 sequences (100000065 bp) in 3.156 CPU sec, 3.428 real sec...
[M::process] read 676264 sequences (100000015 bp) in 2.218 CPU sec, 2.268 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287463, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (240.61, 122.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 4364, 4364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13058)
[M::mem_pestat] mean and std.dev: (2192.30, 2171.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17405)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676296 reads in 253.446 CPU sec, 8.133 real sec
[M::mem_process_seqs] Processed 676344 reads in 149.704 CPU sec, 4.773 real sec
sorting block 175, #reads = 1079413 mem = 536879850 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287133, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (239.97, 122.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676280 sequences (100000053 bp) in 2.973 CPU sec, 3.283 real sec...
[M::process] read 676294 sequences (100000264 bp) in 2.243 CPU sec, 2.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287424, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (241.39, 124.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676270 reads in 239.513 CPU sec, 7.695 real sec
[M::mem_process_seqs] Processed 676264 reads in 135.877 CPU sec, 4.322 real sec
sorting block 176, #reads = 1081044 mem = 536879351 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286874, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (241.17, 123.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 35, 51)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 139)
[M::mem_pestat] mean and std.dev: (24.78, 19.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 183)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676270 sequences (100000057 bp) in 3.073 CPU sec, 3.781 real sec...
[M::process] read 676438 sequences (100000215 bp) in 2.016 CPU sec, 2.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287242, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (240.66, 123.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676280 reads in 240.318 CPU sec, 7.722 real sec
[M::mem_process_seqs] Processed 676294 reads in 138.168 CPU sec, 4.387 real sec
sorting block 177, #reads = 1081719 mem = 536878755 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287494, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (240.99, 123.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676562 sequences (100000187 bp) in 2.896 CPU sec, 3.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287263, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.26, 121.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676838 sequences (100000218 bp) in 2.039 CPU sec, 2.108 real sec...
[M::mem_process_seqs] Processed 676270 reads in 234.000 CPU sec, 7.581 real sec
sorting block 178, #reads = 1081931 mem = 536881644 peak = 1287417856
[M::mem_process_seqs] Processed 676438 reads in 133.427 CPU sec, 4.251 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287512, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.03, 114.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 179, #reads = 1081880 mem = 536877530 peak = 1287417856
[M::process] read 676716 sequences (100000158 bp) in 3.016 CPU sec, 3.425 real sec...
[M::process] read 676668 sequences (100000143 bp) in 2.214 CPU sec, 2.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287730, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.72, 113.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676562 reads in 231.475 CPU sec, 7.489 real sec
[M::mem_process_seqs] Processed 676838 reads in 132.079 CPU sec, 4.215 real sec
sorting block 180, #reads = 1081881 mem = 536878985 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287781, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.91, 114.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676734 sequences (100000138 bp) in 2.967 CPU sec, 3.469 real sec...
[M::process] read 676614 sequences (100000084 bp) in 2.363 CPU sec, 2.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287127, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.23, 115.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676716 reads in 238.309 CPU sec, 7.672 real sec
[M::mem_process_seqs] Processed 676668 reads in 137.506 CPU sec, 4.370 real sec
sorting block 181, #reads = 1082148 mem = 536877995 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287752, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.21, 114.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676604 sequences (100000105 bp) in 3.037 CPU sec, 3.860 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287188, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.91, 114.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 38, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (108.75, 161.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676698 sequences (100000106 bp) in 2.248 CPU sec, 2.253 real sec...
[M::mem_process_seqs] Processed 676734 reads in 241.347 CPU sec, 7.861 real sec
[M::mem_process_seqs] Processed 676614 reads in 135.536 CPU sec, 4.339 real sec
sorting block 182, #reads = 1081085 mem = 536878513 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287749, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.76, 116.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 35, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1295)
[M::mem_pestat] mean and std.dev: (130.43, 206.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1726)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676838 sequences (100000058 bp) in 3.030 CPU sec, 3.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287804, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.86, 113.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676604 reads in 224.197 CPU sec, 7.246 real sec
sorting block 183, #reads = 1082496 mem = 536877497 peak = 1287417856
[M::process] read 676728 sequences (100000294 bp) in 2.223 CPU sec, 2.294 real sec...
[M::mem_process_seqs] Processed 676698 reads in 122.914 CPU sec, 3.917 real sec
sorting block 184, #reads = 1081661 mem = 536881131 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288435, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.51, 110.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676882 sequences (100000277 bp) in 3.309 CPU sec, 3.504 real sec...
[M::process] read 676802 sequences (100000133 bp) in 2.226 CPU sec, 2.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287543, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.11, 111.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676838 reads in 239.971 CPU sec, 7.681 real sec
[M::mem_process_seqs] Processed 676728 reads in 140.824 CPU sec, 4.468 real sec
sorting block 185, #reads = 1080303 mem = 536880304 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287502, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.65, 112.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676810 sequences (100000029 bp) in 3.159 CPU sec, 3.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287577, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.90, 112.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676660 sequences (100000067 bp) in 2.333 CPU sec, 2.360 real sec...
[M::mem_process_seqs] Processed 676882 reads in 234.181 CPU sec, 7.502 real sec
[M::mem_process_seqs] Processed 676802 reads in 137.506 CPU sec, 4.342 real sec
sorting block 186, #reads = 1080573 mem = 536878768 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287261, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.45, 113.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676780 sequences (100000282 bp) in 2.951 CPU sec, 3.246 real sec...
[M::process] read 676912 sequences (100000204 bp) in 2.284 CPU sec, 2.293 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287786, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (230.85, 115.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676810 reads in 230.180 CPU sec, 7.356 real sec
[M::mem_process_seqs] Processed 676660 reads in 129.195 CPU sec, 4.083 real sec
sorting block 187, #reads = 1081626 mem = 536882561 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287499, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.81, 114.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 188, #reads = 1081547 mem = 536876584 peak = 1287417856
[M::process] read 677346 sequences (100000252 bp) in 3.127 CPU sec, 3.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288231, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.46, 111.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676780 reads in 222.587 CPU sec, 7.182 real sec
[M::process] read 677056 sequences (100000200 bp) in 2.318 CPU sec, 2.399 real sec...
[M::mem_process_seqs] Processed 676912 reads in 132.611 CPU sec, 4.257 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288148, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.28, 109.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 38, 828)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2464)
[M::mem_pestat] mean and std.dev: (382.54, 398.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3282)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 189, #reads = 1079873 mem = 536879323 peak = 1287417856
[M::process] read 677068 sequences (100000130 bp) in 3.273 CPU sec, 3.810 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288480, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (229.94, 112.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 89, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1917)
[M::mem_pestat] mean and std.dev: (216.44, 274.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2555)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677346 reads in 228.269 CPU sec, 7.352 real sec
[M::mem_process_seqs] Processed 677056 reads in 131.013 CPU sec, 4.126 real sec
[M::process] read 677002 sequences (100000072 bp) in 2.453 CPU sec, 2.486 real sec...
sorting block 190, #reads = 1081661 mem = 536878786 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288284, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.45, 111.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676840 sequences (100000252 bp) in 2.659 CPU sec, 3.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288319, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.97, 114.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 9, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (54.92, 93.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677068 reads in 227.942 CPU sec, 7.416 real sec
[M::process] read 676670 sequences (100000243 bp) in 2.252 CPU sec, 2.273 real sec...
[M::mem_process_seqs] Processed 677002 reads in 131.237 CPU sec, 4.204 real sec
sorting block 191, #reads = 1082888 mem = 536882842 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287596, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.51, 114.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 12, 35)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 87)
[M::mem_pestat] mean and std.dev: (14.25, 10.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 113)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676814 sequences (100000114 bp) in 3.156 CPU sec, 3.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288271, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.86, 114.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676840 reads in 226.090 CPU sec, 7.289 real sec
[M::process] read 676716 sequences (100000117 bp) in 2.275 CPU sec, 2.284 real sec...
[M::mem_process_seqs] Processed 676670 reads in 129.313 CPU sec, 4.111 real sec
sorting block 192, #reads = 1082087 mem = 536877900 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287841, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.63, 113.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 28, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282)
[M::mem_pestat] mean and std.dev: (31.85, 28.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 372)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 193, #reads = 1080712 mem = 536880353 peak = 1287417856
[M::process] read 676558 sequences (100000162 bp) in 3.232 CPU sec, 3.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288407, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.53, 114.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676814 reads in 224.102 CPU sec, 7.209 real sec
[M::mem_process_seqs] Processed 676716 reads in 130.096 CPU sec, 4.122 real sec
[M::process] read 676748 sequences (100000244 bp) in 2.322 CPU sec, 2.364 real sec...
sorting block 194, #reads = 1082275 mem = 536877709 peak = 1287417856
[M::process] read 676740 sequences (100000192 bp) in 3.310 CPU sec, 3.860 real sec...
[M::process] read 676492 sequences (100000127 bp) in 2.206 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287547, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (232.74, 114.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676748 reads in 144.074 CPU sec, 4.630 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287911, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.46, 114.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 245, 4564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13602)
[M::mem_pestat] mean and std.dev: (1836.77, 2157.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18121)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287149, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.46, 115.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676558 reads in 366.927 CPU sec, 11.829 real sec
sorting block 195, #reads = 1080321 mem = 536879063 peak = 1287417856
[M::process] read 676664 sequences (100000102 bp) in 2.320 CPU sec, 2.335 real sec...
[M::mem_process_seqs] Processed 676740 reads in 145.947 CPU sec, 4.665 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287677, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.64, 116.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 196, #reads = 1080436 mem = 536878953 peak = 1287417856
[M::process] read 676738 sequences (100000197 bp) in 2.701 CPU sec, 3.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287810, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.28, 113.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676492 reads in 232.997 CPU sec, 7.573 real sec
[M::process] read 676624 sequences (100000035 bp) in 2.349 CPU sec, 2.406 real sec...
[M::mem_process_seqs] Processed 676664 reads in 134.373 CPU sec, 4.331 real sec
sorting block 197, #reads = 1081848 mem = 536875782 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288067, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.27, 114.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676612 sequences (100000189 bp) in 2.608 CPU sec, 3.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288007, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.66, 114.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 189, 4230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12588)
[M::mem_pestat] mean and std.dev: (1317.92, 1797.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16767)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676566 sequences (100000042 bp) in 2.164 CPU sec, 2.170 real sec...
[M::mem_process_seqs] Processed 676738 reads in 223.605 CPU sec, 7.236 real sec
[M::mem_process_seqs] Processed 676624 reads in 131.321 CPU sec, 4.185 real sec
sorting block 198, #reads = 1080732 mem = 536876733 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287986, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.25, 114.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676402 sequences (100000113 bp) in 2.780 CPU sec, 3.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287813, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.80, 117.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676612 reads in 218.968 CPU sec, 7.057 real sec
[M::process] read 676560 sequences (100000231 bp) in 2.461 CPU sec, 2.475 real sec...
[M::mem_process_seqs] Processed 676566 reads in 124.659 CPU sec, 3.928 real sec
sorting block 199, #reads = 1079570 mem = 536879306 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287424, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.08, 119.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676452 sequences (100000262 bp) in 2.876 CPU sec, 3.180 real sec...
sorting block 200, #reads = 1079055 mem = 536878276 peak = 1287417856
[M::process] read 676490 sequences (100000231 bp) in 2.080 CPU sec, 2.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287875, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.62, 119.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676402 reads in 255.087 CPU sec, 8.249 real sec
[M::mem_process_seqs] Processed 676560 reads in 160.195 CPU sec, 5.135 real sec
sorting block 201, #reads = 1075446 mem = 536879346 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287380, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.47, 120.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676614 sequences (100000295 bp) in 3.124 CPU sec, 3.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287061, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.58, 121.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676538 sequences (100000084 bp) in 2.273 CPU sec, 2.348 real sec...
[M::mem_process_seqs] Processed 676452 reads in 238.777 CPU sec, 7.732 real sec
[M::mem_process_seqs] Processed 676490 reads in 139.984 CPU sec, 4.499 real sec
sorting block 202, #reads = 1072373 mem = 536877420 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287097, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.02, 120.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676314 sequences (100000150 bp) in 3.047 CPU sec, 3.582 real sec...
[M::process] read 676454 sequences (100000216 bp) in 2.128 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287406, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.59, 121.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676614 reads in 240.725 CPU sec, 7.760 real sec
[M::mem_process_seqs] Processed 676538 reads in 137.116 CPU sec, 4.383 real sec
sorting block 203, #reads = 1081822 mem = 536880741 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287216, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.50, 121.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676440 sequences (100000083 bp) in 2.908 CPU sec, 3.566 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288026, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.00, 122.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676314 reads in 229.120 CPU sec, 7.474 real sec
[M::process] read 676534 sequences (100000225 bp) in 2.456 CPU sec, 2.477 real sec...
[M::mem_process_seqs] Processed 676454 reads in 130.845 CPU sec, 4.208 real sec
sorting block 204, #reads = 1081903 mem = 536879576 peak = 1287417856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287994, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.54, 121.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 205, #reads = 1080040 mem = 536878194 peak = 1288577024
[M::process] read 676490 sequences (100000051 bp) in 3.253 CPU sec, 3.599 real sec...
[M::process] read 676464 sequences (100000171 bp) in 2.149 CPU sec, 2.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287431, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.16, 121.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 16, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (199.92, 299.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1399)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676440 reads in 243.906 CPU sec, 7.808 real sec
[M::mem_process_seqs] Processed 676534 reads in 141.062 CPU sec, 4.482 real sec
sorting block 206, #reads = 1077806 mem = 536879004 peak = 1288577024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286763, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.29, 122.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676568 sequences (100000226 bp) in 3.087 CPU sec, 3.337 real sec...
[M::process] read 676574 sequences (100000070 bp) in 2.203 CPU sec, 2.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286742, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.16, 121.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676490 reads in 244.439 CPU sec, 7.841 real sec
[M::mem_process_seqs] Processed 676464 reads in 138.603 CPU sec, 4.414 real sec
sorting block 207, #reads = 1081958 mem = 536877762 peak = 1288577024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287216, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.17, 121.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 387, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1546)
[M::mem_pestat] mean and std.dev: (355.16, 225.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1991)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676602 sequences (100000143 bp) in 2.826 CPU sec, 3.184 real sec...
[M::process] read 676774 sequences (100000065 bp) in 2.168 CPU sec, 2.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287039, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.22, 119.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676568 reads in 243.451 CPU sec, 7.839 real sec
sorting block 208, #reads = 1081610 mem = 536879277 peak = 1288577024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287340, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.33, 115.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676602 reads in 127.206 CPU sec, 4.061 real sec
[M::process] read 676826 sequences (100000239 bp) in 2.405 CPU sec, 2.494 real sec...
[M::mem_process_seqs] Processed 676574 reads in 314.757 CPU sec, 10.043 real sec
sorting block 209, #reads = 1082330 mem = 536878974 peak = 1288577024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287770, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.82, 113.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676896 sequences (100000162 bp) in 3.063 CPU sec, 3.269 real sec...
[M::process] read 676808 sequences (100000121 bp) in 2.191 CPU sec, 2.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288264, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.51, 113.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 31, 147)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435)
[M::mem_pestat] mean and std.dev: (54.17, 68.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 579)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676774 reads in 247.788 CPU sec, 7.924 real sec
[M::mem_process_seqs] Processed 676826 reads in 135.057 CPU sec, 4.316 real sec
sorting block 210, #reads = 1082583 mem = 536878802 peak = 1288577024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287667, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.06, 112.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676496 sequences (100000187 bp) in 2.875 CPU sec, 3.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287697, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.12, 114.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676896 reads in 235.847 CPU sec, 7.659 real sec
[M::process] read 676620 sequences (100000130 bp) in 2.093 CPU sec, 2.256 real sec...
[M::mem_process_seqs] Processed 676808 reads in 133.803 CPU sec, 4.317 real sec
sorting block 211, #reads = 1082708 mem = 536879835 peak = 1288577024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287244, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.65, 114.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 212, #reads = 1082315 mem = 536876805 peak = 1288577024
[M::process] read 676756 sequences (100000152 bp) in 3.175 CPU sec, 3.579 real sec...
[M::process] read 676642 sequences (100000197 bp) in 2.110 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287504, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.11, 114.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676496 reads in 233.043 CPU sec, 7.482 real sec
[M::mem_process_seqs] Processed 676620 reads in 139.047 CPU sec, 4.415 real sec
sorting block 213, #reads = 1081976 mem = 536881491 peak = 1288577024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287643, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.25, 114.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676756 sequences (100000140 bp) in 3.067 CPU sec, 3.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287796, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.62, 116.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676756 reads in 220.511 CPU sec, 7.073 real sec
[M::process] read 676742 sequences (100000260 bp) in 2.159 CPU sec, 2.172 real sec...
[M::mem_process_seqs] Processed 676642 reads in 129.362 CPU sec, 4.097 real sec
sorting block 214, #reads = 1080712 mem = 536880125 peak = 1288577024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288345, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.31, 113.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676868 sequences (100000221 bp) in 3.160 CPU sec, 3.667 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288011, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.51, 113.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676756 reads in 230.487 CPU sec, 7.449 real sec
[M::process] read 676720 sequences (100000199 bp) in 2.298 CPU sec, 2.306 real sec...
[M::mem_process_seqs] Processed 676742 reads in 134.247 CPU sec, 4.272 real sec
sorting block 215, #reads = 1081327 mem = 536879263 peak = 1288577024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287184, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.05, 113.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676794 sequences (100000031 bp) in 2.938 CPU sec, 3.495 real sec...
[M::process] read 676604 sequences (100000151 bp) in 2.195 CPU sec, 2.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287758, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.59, 112.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676868 reads in 231.344 CPU sec, 7.424 real sec
[M::mem_process_seqs] Processed 676720 reads in 133.143 CPU sec, 4.204 real sec
sorting block 216, #reads = 1082236 mem = 536879475 peak = 1288577024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287367, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.86, 113.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 217, #reads = 1080951 mem = 536879121 peak = 1290719232
[M::process] read 676782 sequences (100000207 bp) in 2.816 CPU sec, 3.178 real sec...
[M::process] read 676940 sequences (100000043 bp) in 2.301 CPU sec, 2.353 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288140, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (232.69, 116.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676794 reads in 236.161 CPU sec, 7.652 real sec
[M::mem_process_seqs] Processed 676604 reads in 146.637 CPU sec, 4.695 real sec
sorting block 218, #reads = 1077677 mem = 536878661 peak = 1290719232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287526, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.62, 113.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677122 sequences (100000142 bp) in 2.844 CPU sec, 3.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288061, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (228.57, 112.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676782 reads in 216.983 CPU sec, 7.059 real sec
[M::process] read 677176 sequences (100000111 bp) in 2.300 CPU sec, 2.305 real sec...
[M::mem_process_seqs] Processed 676940 reads in 129.174 CPU sec, 4.172 real sec
sorting block 219, #reads = 1075725 mem = 536880094 peak = 1290719232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287720, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.98, 109.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 409, 676)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1790)
[M::mem_pestat] mean and std.dev: (413.77, 331.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2347)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676896 sequences (100000071 bp) in 2.989 CPU sec, 3.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288054, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (229.67, 111.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676976 sequences (100000027 bp) in 2.248 CPU sec, 2.266 real sec...
[M::mem_process_seqs] Processed 677122 reads in 230.258 CPU sec, 7.415 real sec
[M::mem_process_seqs] Processed 677176 reads in 131.698 CPU sec, 4.212 real sec
sorting block 220, #reads = 1081174 mem = 536879008 peak = 1290719232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288062, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.12, 112.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676770 sequences (100000187 bp) in 3.188 CPU sec, 3.472 real sec...
[M::process] read 676792 sequences (100000204 bp) in 2.178 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287884, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.32, 113.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676896 reads in 230.834 CPU sec, 7.445 real sec
[M::mem_process_seqs] Processed 676976 reads in 133.168 CPU sec, 4.270 real sec
sorting block 221, #reads = 1082341 mem = 536876990 peak = 1290719232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287896, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.72, 114.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 222, #reads = 1082080 mem = 536879256 peak = 1290719232
[M::process] read 676764 sequences (100000120 bp) in 2.806 CPU sec, 2.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287567, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.47, 114.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676608 sequences (100000132 bp) in 2.202 CPU sec, 2.211 real sec...
[M::mem_process_seqs] Processed 676770 reads in 233.457 CPU sec, 7.561 real sec
[M::mem_process_seqs] Processed 676792 reads in 134.859 CPU sec, 4.252 real sec
sorting block 223, #reads = 1082146 mem = 536879418 peak = 1290719232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287349, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.87, 115.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 176, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (124.33, 124.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1402)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676720 sequences (100000128 bp) in 2.725 CPU sec, 2.887 real sec...
[M::process] read 676830 sequences (100000291 bp) in 2.063 CPU sec, 2.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287199, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.07, 115.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676764 reads in 235.606 CPU sec, 7.547 real sec
[M::mem_process_seqs] Processed 676608 reads in 132.958 CPU sec, 4.221 real sec
sorting block 224, #reads = 1081971 mem = 536878223 peak = 1290719232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286971, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.08, 115.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 198, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1356)
[M::mem_pestat] mean and std.dev: (241.00, 187.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1783)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676550 sequences (100000294 bp) in 2.753 CPU sec, 3.234 real sec...
[M::process] read 676672 sequences (100000278 bp) in 2.157 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287751, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.64, 113.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 385, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131)
[M::mem_pestat] mean and std.dev: (299.64, 306.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1524)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676720 reads in 235.236 CPU sec, 7.617 real sec
[M::mem_process_seqs] Processed 676830 reads in 134.947 CPU sec, 4.304 real sec
sorting block 225, #reads = 1081881 mem = 536879401 peak = 1290719232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287354, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.70, 114.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676578 sequences (100000128 bp) in 2.964 CPU sec, 3.295 real sec...
[M::process] read 676558 sequences (100000111 bp) in 1.877 CPU sec, 1.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287843, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.07, 115.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676550 reads in 235.627 CPU sec, 7.582 real sec
[M::mem_process_seqs] Processed 676672 reads in 132.768 CPU sec, 4.220 real sec
sorting block 226, #reads = 1082088 mem = 536881771 peak = 1290719232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286963, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.75, 114.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 227, #reads = 1081960 mem = 536879771 peak = 1299714048
[M::process] read 676480 sequences (100000148 bp) in 2.821 CPU sec, 3.254 real sec...
[M::process] read 676500 sequences (100000014 bp) in 2.215 CPU sec, 2.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287117, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.00, 115.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676578 reads in 240.882 CPU sec, 7.790 real sec
[M::mem_process_seqs] Processed 676558 reads in 137.025 CPU sec, 4.398 real sec
sorting block 228, #reads = 1081996 mem = 536877017 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287337, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.40, 115.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676548 sequences (100000276 bp) in 3.021 CPU sec, 3.625 real sec...
[M::process] read 676352 sequences (100000285 bp) in 2.181 CPU sec, 2.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287650, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.34, 116.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 32, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353)
[M::mem_pestat] mean and std.dev: (175.76, 252.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1803)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676480 reads in 233.750 CPU sec, 7.561 real sec
[M::mem_process_seqs] Processed 676500 reads in 134.306 CPU sec, 4.298 real sec
sorting block 229, #reads = 1081226 mem = 536879303 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286954, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.71, 118.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 100, 722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2114)
[M::mem_pestat] mean and std.dev: (253.31, 285.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2810)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676268 sequences (100000210 bp) in 2.670 CPU sec, 2.904 real sec...
[M::process] read 676408 sequences (100000079 bp) in 2.214 CPU sec, 2.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287276, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.88, 119.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676548 reads in 228.185 CPU sec, 7.347 real sec
[M::mem_process_seqs] Processed 676352 reads in 137.736 CPU sec, 4.369 real sec
sorting block 230, #reads = 1079362 mem = 536879686 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287217, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.43, 120.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676324 sequences (100000049 bp) in 2.992 CPU sec, 3.192 real sec...
[M::process] read 676218 sequences (100000017 bp) in 2.103 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287141, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.75, 119.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676268 reads in 238.244 CPU sec, 7.721 real sec
sorting block 231, #reads = 1080900 mem = 536879165 peak = 1299714048
[M::mem_process_seqs] Processed 676408 reads in 137.808 CPU sec, 4.440 real sec
sorting block 232, #reads = 1076464 mem = 536879075 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287606, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.16, 120.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 159, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143)
[M::mem_pestat] mean and std.dev: (169.89, 132.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1471)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676360 sequences (100000161 bp) in 2.871 CPU sec, 3.370 real sec...
[M::process] read 676208 sequences (100000258 bp) in 2.244 CPU sec, 2.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286654, 21, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.46, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 640, 1147)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3371)
[M::mem_pestat] mean and std.dev: (534.95, 420.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4483)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676324 reads in 253.773 CPU sec, 8.228 real sec
[M::mem_process_seqs] Processed 676218 reads in 135.146 CPU sec, 4.288 real sec
sorting block 233, #reads = 1073302 mem = 536877686 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286744, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.89, 120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676350 sequences (100000095 bp) in 2.941 CPU sec, 3.115 real sec...
[M::process] read 676334 sequences (100000198 bp) in 2.188 CPU sec, 2.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287272, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (240.31, 121.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676360 reads in 241.264 CPU sec, 7.741 real sec
[M::mem_process_seqs] Processed 676208 reads in 133.241 CPU sec, 4.222 real sec
sorting block 234, #reads = 1081216 mem = 536879825 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286918, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.04, 121.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 210, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1573)
[M::mem_pestat] mean and std.dev: (284.30, 229.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2086)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676278 sequences (100000111 bp) in 2.759 CPU sec, 2.939 real sec...
[M::process] read 676356 sequences (100000232 bp) in 2.200 CPU sec, 2.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287115, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (240.48, 122.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 351, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1913)
[M::mem_pestat] mean and std.dev: (367.40, 430.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2549)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676350 reads in 262.625 CPU sec, 8.487 real sec
[M::mem_process_seqs] Processed 676334 reads in 155.173 CPU sec, 4.948 real sec
sorting block 235, #reads = 1078980 mem = 536877709 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286545, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (240.02, 122.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (90, 925, 925)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2595)
[M::mem_pestat] mean and std.dev: (546.57, 437.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3430)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 236, #reads = 1076728 mem = 536882893 peak = 1299714048
[M::process] read 676198 sequences (100000295 bp) in 2.983 CPU sec, 3.257 real sec...
[M::process] read 676242 sequences (100000028 bp) in 2.138 CPU sec, 2.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287236, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (240.53, 122.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676278 reads in 241.814 CPU sec, 7.855 real sec
[M::mem_process_seqs] Processed 676356 reads in 145.997 CPU sec, 4.624 real sec
sorting block 237, #reads = 1078560 mem = 536878866 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287013, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (241.11, 122.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676358 sequences (100000151 bp) in 2.998 CPU sec, 3.341 real sec...
[M::process] read 676344 sequences (100000230 bp) in 2.288 CPU sec, 2.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286991, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.93, 121.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 61, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123)
[M::mem_pestat] mean and std.dev: (159.00, 239.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1494)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676198 reads in 248.423 CPU sec, 7.967 real sec
[M::mem_process_seqs] Processed 676242 reads in 140.209 CPU sec, 4.448 real sec
sorting block 238, #reads = 1082339 mem = 536879450 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286749, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.49, 121.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676842 sequences (100000263 bp) in 3.477 CPU sec, 3.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287304, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.65, 120.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676358 reads in 239.002 CPU sec, 7.803 real sec
[M::process] read 676644 sequences (100000032 bp) in 2.365 CPU sec, 2.425 real sec...
[M::mem_process_seqs] Processed 676344 reads in 132.711 CPU sec, 4.311 real sec
sorting block 239, #reads = 1080396 mem = 536879985 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287602, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.29, 113.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676694 sequences (100000260 bp) in 2.907 CPU sec, 3.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287867, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.70, 113.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (55, 537, 727)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2071)
[M::mem_pestat] mean and std.dev: (373.92, 310.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2743)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676512 sequences (100000215 bp) in 2.155 CPU sec, 2.190 real sec...
[M::mem_process_seqs] Processed 676842 reads in 230.652 CPU sec, 7.423 real sec
[M::mem_process_seqs] Processed 676644 reads in 132.761 CPU sec, 4.216 real sec
sorting block 240, #reads = 1079982 mem = 536877310 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287531, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.26, 115.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 241, #reads = 1080385 mem = 536878573 peak = 1299714048
[M::process] read 676598 sequences (100000147 bp) in 3.149 CPU sec, 3.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287527, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.84, 115.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676754 sequences (100000247 bp) in 2.263 CPU sec, 2.268 real sec...
[M::mem_process_seqs] Processed 676694 reads in 239.311 CPU sec, 7.756 real sec
[M::mem_process_seqs] Processed 676512 reads in 135.335 CPU sec, 4.323 real sec
sorting block 242, #reads = 1081319 mem = 536878140 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287688, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.84, 114.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676696 sequences (100000214 bp) in 2.721 CPU sec, 3.013 real sec...
[M::process] read 676512 sequences (100000276 bp) in 2.263 CPU sec, 2.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287330, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.20, 114.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 230, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (183.38, 171.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 869)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676598 reads in 238.998 CPU sec, 7.680 real sec
[M::mem_process_seqs] Processed 676754 reads in 136.255 CPU sec, 4.310 real sec
sorting block 243, #reads = 1081840 mem = 536878263 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287740, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.05, 115.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 60, 757)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2267)
[M::mem_pestat] mean and std.dev: (265.58, 348.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3022)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676472 sequences (100000054 bp) in 3.006 CPU sec, 3.248 real sec...
[M::process] read 676682 sequences (100000276 bp) in 2.047 CPU sec, 2.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287590, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.59, 117.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676696 reads in 236.272 CPU sec, 7.596 real sec
[M::mem_process_seqs] Processed 676512 reads in 134.003 CPU sec, 4.267 real sec
sorting block 244, #reads = 1082583 mem = 536880346 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287605, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.62, 116.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676442 sequences (100000216 bp) in 2.958 CPU sec, 3.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287610, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::process] read 676368 sequences (100000165 bp) in 2.223 CPU sec, 2.260 real sec...
[M::mem_pestat] mean and std.dev: (234.51, 117.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676472 reads in 238.657 CPU sec, 7.800 real sec
[M::mem_process_seqs] Processed 676682 reads in 138.605 CPU sec, 4.480 real sec
sorting block 245, #reads = 1082449 mem = 536878301 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287538, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.30, 113.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 246, #reads = 1081488 mem = 536876067 peak = 1299714048
[M::process] read 676522 sequences (100000055 bp) in 3.150 CPU sec, 3.325 real sec...
[M::process] read 676522 sequences (100000201 bp) in 2.236 CPU sec, 2.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287156, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.50, 115.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676442 reads in 238.227 CPU sec, 7.685 real sec
[M::mem_process_seqs] Processed 676368 reads in 137.437 CPU sec, 4.376 real sec
sorting block 247, #reads = 1081939 mem = 536879956 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288042, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.06, 116.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 892, 892)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2192)
[M::mem_pestat] mean and std.dev: (625.07, 343.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2842)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676304 sequences (100000237 bp) in 3.190 CPU sec, 3.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287545, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.28, 116.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676400 sequences (100000052 bp) in 2.321 CPU sec, 2.392 real sec...
[M::mem_process_seqs] Processed 676522 reads in 237.863 CPU sec, 7.643 real sec
[M::mem_process_seqs] Processed 676522 reads in 137.072 CPU sec, 4.367 real sec
sorting block 248, #reads = 1081982 mem = 536877164 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287049, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.52, 120.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676544 sequences (100000118 bp) in 3.375 CPU sec, 3.712 real sec...
[M::process] read 8098 sequences (1196653 bp) in 0.111 CPU sec, 0.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287525, 20, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.23, 119.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 29, 45)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 117)
[M::mem_pestat] mean and std.dev: (17.44, 14.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 153)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676304 reads in 235.391 CPU sec, 7.701 real sec
[M::mem_process_seqs] Processed 676400 reads in 133.088 CPU sec, 4.332 real sec
sorting block 249, #reads = 1082077 mem = 536880040 peak = 1299714048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 3408, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 179, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (235.36, 121.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 8098 reads in 1.693 CPU sec, 0.061 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287361, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.45, 119.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676544 reads in 122.792 CPU sec, 4.079 real sec
sorting block 250, #reads = 1081868 mem = 536879499 peak = 1299714048
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 269917672
overall: 69821247488 mem 50016.358 user 753.528 sys 1661.616 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L002\tPU:L002\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 T21-D0003376_R1.fastq.gz T21-D0003376_R2.fastq.gz
[main] Real time: 1661.653 sec; CPU: 50769.890 sec
sorting block 251, #reads = 564369 mem = 280276258 peak = 1299714048
uniq header count 1
merging 252 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31 max 96
algo: util-sort
license: sentieon:util=1
output file size: 9309505557
output reads: 272016611
bam_mem_sort: 252 calls 301.714 user 5.753 sys 314.832 real
bam_write: 252 calls 197.229 user 14.809 sys 414.835 real
execute: 1 calls 104.005 user 4.935 sys 1792.157 real
merge_files: 1 calls 103.975 user 4.897 sys 129.565 real
parse_chunk: 4025 calls 365.606 user 68.270 sys 517.436 real
read_chunk: 10833 calls 162.185 user 75.190 sys 1637.659 real
sort_block: 1 calls 109.242 user 74.411 sys 1568.384 real
write_chunk: 6806 calls 102.957 user 3.738 sys 125.149 real
overall: 2986565632 mem 4329.290 user 213.497 sys 1792.179 real
ls: cannot access '*.cram': No such file or directory