cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2989.758 CPU sec, 118.361 real sec
[M::process] read 676920 sequences (100000040 bp) in 1.270 CPU sec, 1.277 real sec...
[M::process] read 677118 sequences (100000066 bp) in 1.639 CPU sec, 1.638 real sec...
[M::process] read 677080 sequences (100000246 bp) in 1.589 CPU sec, 1.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288417, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (227.23, 107.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 9, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1170)
[M::mem_pestat] mean and std.dev: (66.82, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1559)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676930 sequences (100000177 bp) in 1.554 CPU sec, 1.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288458, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (226.14, 106.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288227, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.36, 106.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676920 reads in 332.822 CPU sec, 10.764 real sec
[M::mem_process_seqs] Processed 677080 reads in 223.859 CPU sec, 7.395 real sec
[M::mem_process_seqs] Processed 677118 reads in 235.803 CPU sec, 7.828 real sec
sorting block 0, #reads = 1082508 mem = 536878099 peak = 580878336
[M::process] read 676870 sequences (100000188 bp) in 1.968 CPU sec, 2.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287903, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.84, 108.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 176, 613)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1821)
[M::mem_pestat] mean and std.dev: (328.21, 351.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2425)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676872 sequences (100000295 bp) in 1.837 CPU sec, 2.024 real sec...
[M::process] read 676752 sequences (100000192 bp) in 1.363 CPU sec, 1.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288521, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (229.19, 109.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676930 reads in 222.399 CPU sec, 7.207 real sec
sorting block 1, #reads = 1082406 mem = 536880430 peak = 845119488
[M::mem_process_seqs] Processed 676870 reads in 128.472 CPU sec, 4.185 real sec
sorting block 2, #reads = 1082364 mem = 536880256 peak = 846868480
[M::process] read 676808 sequences (100000036 bp) in 2.104 CPU sec, 2.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287767, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.75, 109.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676626 sequences (100000043 bp) in 1.376 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288343, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.33, 108.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255)
[M::mem_pestat] mean and std.dev: (13.50, 27.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 339)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287964, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (229.42, 110.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 574, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1957)
[M::mem_pestat] mean and std.dev: (337.44, 316.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2608)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676872 reads in 328.094 CPU sec, 10.636 real sec
[M::mem_process_seqs] Processed 676752 reads in 233.894 CPU sec, 7.614 real sec
[M::mem_process_seqs] Processed 676808 reads in 245.968 CPU sec, 8.102 real sec
sorting block 3, #reads = 1082393 mem = 536880389 peak = 846868480
[M::process] read 676532 sequences (100000078 bp) in 2.093 CPU sec, 2.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287922, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.88, 113.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 63, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 149)
[M::mem_pestat] mean and std.dev: (47.55, 22.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 187)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676506 sequences (100000130 bp) in 2.372 CPU sec, 2.665 real sec...
sorting block 4, #reads = 1082365 mem = 536879746 peak = 846868480
[M::process] read 676288 sequences (100000072 bp) in 1.476 CPU sec, 1.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287749, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.48, 113.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 316, 1193)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3559)
[M::mem_pestat] mean and std.dev: (330.62, 423.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4742)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676626 reads in 233.062 CPU sec, 7.575 real sec
[M::mem_process_seqs] Processed 676532 reads in 135.136 CPU sec, 4.389 real sec
sorting block 5, #reads = 1082425 mem = 536878747 peak = 846868480
[M::process] read 676344 sequences (100000029 bp) in 2.588 CPU sec, 2.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287898, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.72, 116.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676942 sequences (100000011 bp) in 1.457 CPU sec, 1.469 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288084, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.73, 118.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287772, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.09, 119.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676506 reads in 333.545 CPU sec, 10.800 real sec
[M::mem_process_seqs] Processed 676344 reads in 234.823 CPU sec, 7.659 real sec
[M::mem_process_seqs] Processed 676288 reads in 247.673 CPU sec, 8.135 real sec
sorting block 6, #reads = 1082413 mem = 536877706 peak = 846868480
[M::process] read 677124 sequences (100000236 bp) in 2.557 CPU sec, 2.832 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288306, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.01, 110.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1081901 mem = 536880933 peak = 846868480
[M::process] read 677122 sequences (100000092 bp) in 2.110 CPU sec, 2.339 real sec...
[M::process] read 676892 sequences (100000079 bp) in 1.431 CPU sec, 1.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288262, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.44, 107.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676942 reads in 230.677 CPU sec, 7.556 real sec
[M::mem_process_seqs] Processed 677124 reads in 135.725 CPU sec, 4.403 real sec
sorting block 8, #reads = 1082052 mem = 536878394 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288384, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.91, 107.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676954 sequences (100000263 bp) in 2.495 CPU sec, 2.741 real sec...
[M::process] read 676872 sequences (100000198 bp) in 1.613 CPU sec, 1.705 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287684, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.62, 110.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677122 reads in 231.242 CPU sec, 7.855 real sec
[M::mem_process_seqs] Processed 676892 reads in 130.421 CPU sec, 4.842 real sec
sorting block 9, #reads = 1082669 mem = 536878975 peak = 846868480
[M::process] read 677004 sequences (100000085 bp) in 2.238 CPU sec, 2.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288184, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.88, 111.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 79, 125)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321)
[M::mem_pestat] mean and std.dev: (56.91, 36.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 419)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676686 sequences (100000266 bp) in 1.489 CPU sec, 1.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287940, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.35, 109.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288079, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.88, 109.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676954 reads in 333.373 CPU sec, 10.969 real sec
[M::mem_process_seqs] Processed 676872 reads in 232.860 CPU sec, 7.633 real sec
[M::mem_process_seqs] Processed 677004 reads in 242.202 CPU sec, 7.979 real sec
sorting block 10, #reads = 1082027 mem = 536879366 peak = 846868480
[M::process] read 676648 sequences (100000273 bp) in 2.219 CPU sec, 2.433 real sec...
sorting block 11, #reads = 1082246 mem = 536880440 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287535, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.98, 112.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676642 sequences (100000075 bp) in 2.562 CPU sec, 2.859 real sec...
[M::process] read 676318 sequences (100000113 bp) in 1.468 CPU sec, 1.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287679, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.15, 113.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676686 reads in 234.293 CPU sec, 7.558 real sec
[M::mem_process_seqs] Processed 676648 reads in 131.404 CPU sec, 4.199 real sec
sorting block 12, #reads = 1082042 mem = 536877490 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287897, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.54, 114.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676218 sequences (100000068 bp) in 2.524 CPU sec, 2.897 real sec...
[M::process] read 676666 sequences (100000053 bp) in 1.614 CPU sec, 1.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287834, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.99, 117.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676642 reads in 227.139 CPU sec, 7.311 real sec
[M::mem_process_seqs] Processed 676318 reads in 125.695 CPU sec, 4.058 real sec
sorting block 13, #reads = 1082278 mem = 536879807 peak = 846868480
sorting block 14, #reads = 1082156 mem = 536879185 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287846, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.78, 119.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (63, 388, 1252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3630)
[M::mem_pestat] mean and std.dev: (579.17, 583.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4819)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676876 sequences (100000169 bp) in 2.598 CPU sec, 2.912 real sec...
[M::process] read 676968 sequences (100000267 bp) in 1.674 CPU sec, 1.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288095, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.85, 114.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676218 reads in 221.008 CPU sec, 7.123 real sec
[M::mem_process_seqs] Processed 676666 reads in 121.220 CPU sec, 3.893 real sec
sorting block 15, #reads = 1081948 mem = 536881628 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288526, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.30, 112.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677002 sequences (100000130 bp) in 2.470 CPU sec, 2.707 real sec...
[M::process] read 676798 sequences (100000214 bp) in 1.756 CPU sec, 1.760 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288208, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.76, 108.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676876 reads in 220.767 CPU sec, 7.103 real sec
[M::mem_process_seqs] Processed 676968 reads in 122.614 CPU sec, 3.946 real sec
sorting block 16, #reads = 1082312 mem = 536877721 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288508, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.93, 108.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676654 sequences (100000068 bp) in 2.525 CPU sec, 2.808 real sec...
[M::process] read 677074 sequences (100000212 bp) in 1.479 CPU sec, 1.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288326, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.17, 111.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 211, 727)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2175)
[M::mem_pestat] mean and std.dev: (304.00, 331.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2899)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677002 reads in 223.369 CPU sec, 7.218 real sec
[M::mem_process_seqs] Processed 676798 reads in 126.459 CPU sec, 4.094 real sec
sorting block 17, #reads = 1082328 mem = 536880388 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288574, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.90, 112.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 167, 1088)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2930)
[M::mem_pestat] mean and std.dev: (747.55, 926.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4455)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676640 sequences (100000130 bp) in 2.679 CPU sec, 3.021 real sec...
[M::process] read 676646 sequences (100000132 bp) in 1.599 CPU sec, 1.608 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288369, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.63, 107.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676654 reads in 222.569 CPU sec, 7.185 real sec
[M::mem_process_seqs] Processed 677074 reads in 123.513 CPU sec, 3.986 real sec
sorting block 18, #reads = 1082489 mem = 536878021 peak = 846868480
sorting block 19, #reads = 1082757 mem = 536880787 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288296, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.11, 111.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676610 sequences (100000245 bp) in 2.315 CPU sec, 2.574 real sec...
[M::process] read 676394 sequences (100000190 bp) in 1.588 CPU sec, 1.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287870, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.48, 113.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 158, 158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464)
[M::mem_pestat] mean and std.dev: (85.22, 88.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 617)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676640 reads in 224.984 CPU sec, 7.295 real sec
[M::mem_process_seqs] Processed 676646 reads in 120.274 CPU sec, 3.874 real sec
sorting block 20, #reads = 1082309 mem = 536877183 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287789, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.85, 114.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676422 sequences (100000241 bp) in 2.600 CPU sec, 2.890 real sec...
[M::process] read 676222 sequences (100000141 bp) in 1.745 CPU sec, 1.754 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288115, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.15, 117.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676610 reads in 215.541 CPU sec, 6.942 real sec
[M::mem_process_seqs] Processed 676394 reads in 120.058 CPU sec, 3.853 real sec
sorting block 21, #reads = 1082054 mem = 536880535 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287427, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.14, 117.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676814 sequences (100000113 bp) in 2.399 CPU sec, 2.814 real sec...
[M::process] read 676700 sequences (100000254 bp) in 1.508 CPU sec, 1.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288147, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (240.13, 120.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676422 reads in 213.776 CPU sec, 6.906 real sec
sorting block 22, #reads = 1082214 mem = 536879358 peak = 846868480
[M::process] read 676972 sequences (100000237 bp) in 2.078 CPU sec, 2.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288559, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.40, 109.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676814 reads in 108.542 CPU sec, 3.514 real sec
[M::mem_process_seqs] Processed 676222 reads in 278.435 CPU sec, 8.994 real sec
sorting block 23, #reads = 1082051 mem = 536880395 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288169, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.29, 111.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 34, 89)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235)
[M::mem_pestat] mean and std.dev: (39.09, 31.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 308)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676882 sequences (100000281 bp) in 2.025 CPU sec, 2.221 real sec...
[M::process] read 676822 sequences (100000140 bp) in 1.661 CPU sec, 1.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288831, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.71, 107.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676700 reads in 220.218 CPU sec, 7.115 real sec
[M::mem_process_seqs] Processed 676972 reads in 118.475 CPU sec, 3.822 real sec
sorting block 24, #reads = 1081903 mem = 536879153 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288809, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.67, 109.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676694 sequences (100000103 bp) in 2.130 CPU sec, 2.541 real sec...
[M::process] read 676854 sequences (100000201 bp) in 1.471 CPU sec, 1.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288735, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.65, 110.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676882 reads in 209.015 CPU sec, 6.751 real sec
[M::mem_process_seqs] Processed 676822 reads in 117.651 CPU sec, 3.786 real sec
sorting block 25, #reads = 1082737 mem = 536879445 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287825, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.25, 110.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1082651 mem = 536879501 peak = 846868480
[M::process] read 676762 sequences (100000021 bp) in 2.428 CPU sec, 2.673 real sec...
[M::process] read 676496 sequences (100000270 bp) in 1.633 CPU sec, 1.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288203, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.31, 108.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676694 reads in 213.015 CPU sec, 6.878 real sec
[M::mem_process_seqs] Processed 676854 reads in 120.145 CPU sec, 3.878 real sec
sorting block 27, #reads = 1082696 mem = 536878160 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288378, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.35, 110.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 351, 566)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1616)
[M::mem_pestat] mean and std.dev: (285.38, 259.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2141)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676476 sequences (100000201 bp) in 2.291 CPU sec, 2.505 real sec...
[M::process] read 676298 sequences (100000077 bp) in 1.651 CPU sec, 1.664 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287963, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.64, 112.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676762 reads in 212.852 CPU sec, 6.842 real sec
[M::mem_process_seqs] Processed 676496 reads in 116.898 CPU sec, 3.732 real sec
sorting block 28, #reads = 1082440 mem = 536878161 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287943, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.33, 113.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 1684, 1684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4996)
[M::mem_pestat] mean and std.dev: (961.40, 882.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6652)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676292 sequences (100000028 bp) in 2.468 CPU sec, 2.932 real sec...
[M::process] read 676384 sequences (100000247 bp) in 1.590 CPU sec, 1.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287655, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.98, 116.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676476 reads in 213.345 CPU sec, 6.889 real sec
[M::mem_process_seqs] Processed 676298 reads in 121.055 CPU sec, 3.882 real sec
sorting block 29, #reads = 1081950 mem = 536879003 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287993, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.34, 117.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 94, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (128.73, 137.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1347)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677444 sequences (100000053 bp) in 2.380 CPU sec, 2.680 real sec...
[M::process] read 677424 sequences (100000211 bp) in 1.559 CPU sec, 1.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287887, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.57, 118.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (37, 474, 825)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2401)
[M::mem_pestat] mean and std.dev: (381.27, 350.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3189)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676292 reads in 213.327 CPU sec, 6.902 real sec
[M::mem_process_seqs] Processed 676384 reads in 117.745 CPU sec, 3.809 real sec
sorting block 30, #reads = 1081780 mem = 536881033 peak = 846868480
sorting block 31, #reads = 1082579 mem = 536878459 peak = 846868480
[M::process] read 677258 sequences (100000181 bp) in 2.440 CPU sec, 2.807 real sec...
[M::process] read 677208 sequences (100000071 bp) in 1.509 CPU sec, 1.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288260, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (223.92, 106.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677424 reads in 116.348 CPU sec, 3.727 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288139, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.42, 103.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677082 sequences (100000125 bp) in 1.474 CPU sec, 1.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287561, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.57, 107.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677444 reads in 330.625 CPU sec, 10.678 real sec
[M::mem_process_seqs] Processed 677258 reads in 131.158 CPU sec, 4.260 real sec
sorting block 32, #reads = 1081990 mem = 536878551 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288206, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.78, 109.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676836 sequences (100000159 bp) in 2.637 CPU sec, 3.064 real sec...
sorting block 33, #reads = 1081692 mem = 536880285 peak = 846868480
[M::process] read 676794 sequences (100000137 bp) in 1.471 CPU sec, 1.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288417, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.45, 109.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677208 reads in 220.787 CPU sec, 7.141 real sec
[M::mem_process_seqs] Processed 677082 reads in 124.672 CPU sec, 4.013 real sec
sorting block 34, #reads = 1079906 mem = 536879288 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288397, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.82, 107.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676870 sequences (100000250 bp) in 2.652 CPU sec, 3.013 real sec...
[M::process] read 676806 sequences (100000037 bp) in 1.641 CPU sec, 1.644 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287539, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (227.21, 108.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676836 reads in 222.718 CPU sec, 7.189 real sec
[M::mem_process_seqs] Processed 676794 reads in 127.543 CPU sec, 4.118 real sec
sorting block 35, #reads = 1081254 mem = 536879824 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287635, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.90, 110.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676890 sequences (100000076 bp) in 2.459 CPU sec, 2.756 real sec...
[M::process] read 676654 sequences (100000034 bp) in 1.606 CPU sec, 1.614 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287584, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.50, 110.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676870 reads in 223.185 CPU sec, 7.174 real sec
[M::mem_process_seqs] Processed 676806 reads in 123.657 CPU sec, 3.973 real sec
sorting block 36, #reads = 1082375 mem = 536876674 peak = 846868480
[M::process] read 676504 sequences (100000099 bp) in 2.291 CPU sec, 2.769 real sec...
[M::process] read 677094 sequences (100000017 bp) in 1.709 CPU sec, 1.719 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287620, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.61, 115.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676654 reads in 118.228 CPU sec, 3.773 real sec
sorting block 37, #reads = 1082147 mem = 536878460 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287779, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.03, 112.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287486, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.19, 116.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677328 sequences (100000122 bp) in 1.650 CPU sec, 1.655 real sec...
[M::mem_process_seqs] Processed 676890 reads in 341.376 CPU sec, 11.010 real sec
[M::mem_process_seqs] Processed 676504 reads in 134.582 CPU sec, 4.376 real sec
sorting block 38, #reads = 1082331 mem = 536878840 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288056, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.30, 111.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677254 sequences (100000218 bp) in 2.079 CPU sec, 2.368 real sec...
[M::process] read 677264 sequences (100000266 bp) in 1.513 CPU sec, 1.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288416, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.43, 106.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 92, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (124.06, 109.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677094 reads in 214.714 CPU sec, 6.948 real sec
[M::mem_process_seqs] Processed 677328 reads in 120.722 CPU sec, 3.902 real sec
sorting block 39, #reads = 1081961 mem = 536878675 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288091, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.49, 104.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 695, 1974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5904)
[M::mem_pestat] mean and std.dev: (926.86, 883.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7869)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676974 sequences (100000019 bp) in 2.430 CPU sec, 2.868 real sec...
[M::process] read 677006 sequences (100000040 bp) in 1.445 CPU sec, 1.469 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288716, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.62, 108.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677254 reads in 213.428 CPU sec, 6.900 real sec
sorting block 40, #reads = 1082114 mem = 536879236 peak = 846868480
[M::mem_process_seqs] Processed 677264 reads in 121.383 CPU sec, 3.938 real sec
sorting block 41, #reads = 1081276 mem = 536878081 peak = 846868480
[M::process] read 677114 sequences (100000234 bp) in 1.913 CPU sec, 2.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288347, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.66, 110.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676974 sequences (100000016 bp) in 1.459 CPU sec, 1.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288649, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.47, 105.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288150, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.65, 109.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676974 reads in 315.669 CPU sec, 10.207 real sec
[M::mem_process_seqs] Processed 677114 reads in 220.756 CPU sec, 7.138 real sec
[M::mem_process_seqs] Processed 677006 reads in 230.316 CPU sec, 7.475 real sec
sorting block 42, #reads = 1077704 mem = 536877812 peak = 846868480
[M::process] read 676848 sequences (100000105 bp) in 2.797 CPU sec, 3.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287911, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.72, 108.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 43, #reads = 1079955 mem = 536880554 peak = 846868480
[M::process] read 676956 sequences (100000202 bp) in 2.403 CPU sec, 2.652 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287823, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.74, 109.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 25, 91)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 225)
[M::mem_pestat] mean and std.dev: (40.75, 33.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 292)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676974 reads in 224.108 CPU sec, 7.323 real sec
[M::mem_process_seqs] Processed 676848 reads in 126.040 CPU sec, 4.061 real sec
[M::process] read 676720 sequences (100000015 bp) in 1.755 CPU sec, 1.831 real sec...
sorting block 44, #reads = 1081714 mem = 536877622 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287968, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.68, 109.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676724 sequences (100000214 bp) in 2.191 CPU sec, 2.836 real sec...
[M::process] read 676888 sequences (100000187 bp) in 1.834 CPU sec, 1.894 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287897, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.55, 112.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676956 reads in 217.000 CPU sec, 7.039 real sec
[M::mem_process_seqs] Processed 676720 reads in 120.504 CPU sec, 3.881 real sec
sorting block 45, #reads = 1080740 mem = 536879481 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288181, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.49, 113.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676876 sequences (100000156 bp) in 2.497 CPU sec, 2.812 real sec...
[M::process] read 677094 sequences (100000145 bp) in 1.422 CPU sec, 1.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288254, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.05, 111.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676724 reads in 214.551 CPU sec, 6.966 real sec
[M::mem_process_seqs] Processed 676888 reads in 119.376 CPU sec, 3.873 real sec
sorting block 46, #reads = 1082006 mem = 536881080 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287561, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.48, 108.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677126 sequences (100000036 bp) in 2.061 CPU sec, 2.510 real sec...
[M::process] read 676942 sequences (100000119 bp) in 1.619 CPU sec, 1.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288458, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.13, 108.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (632, 793, 3803)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10145)
[M::mem_pestat] mean and std.dev: (1999.29, 1859.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13316)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676876 reads in 213.395 CPU sec, 6.922 real sec
sorting block 47, #reads = 1079180 mem = 536878842 peak = 846868480
[M::mem_process_seqs] Processed 677094 reads in 120.405 CPU sec, 3.864 real sec
sorting block 48, #reads = 1082275 mem = 536877739 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288096, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.42, 108.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676888 sequences (100000176 bp) in 2.193 CPU sec, 2.554 real sec...
[M::process] read 677028 sequences (100000182 bp) in 1.691 CPU sec, 1.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288141, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (229.17, 110.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677126 reads in 205.520 CPU sec, 6.668 real sec
[M::mem_process_seqs] Processed 676942 reads in 117.279 CPU sec, 3.777 real sec
sorting block 49, #reads = 1082826 mem = 536879519 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287707, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.66, 110.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 58, 1150)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3382)
[M::mem_pestat] mean and std.dev: (306.23, 463.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4498)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677054 sequences (100000238 bp) in 2.401 CPU sec, 2.637 real sec...
[M::process] read 676880 sequences (100000087 bp) in 1.599 CPU sec, 1.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287902, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.77, 106.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676888 reads in 208.418 CPU sec, 6.702 real sec
[M::mem_process_seqs] Processed 677028 reads in 114.732 CPU sec, 3.668 real sec
sorting block 50, #reads = 1077526 mem = 536880911 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288122, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.02, 105.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676856 sequences (100000053 bp) in 2.246 CPU sec, 2.462 real sec...
[M::process] read 676840 sequences (100000151 bp) in 1.417 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287703, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.61, 108.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677054 reads in 215.697 CPU sec, 6.959 real sec
[M::mem_process_seqs] Processed 676880 reads in 122.642 CPU sec, 3.960 real sec
sorting block 51, #reads = 1080632 mem = 536879946 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287721, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.61, 109.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676958 sequences (100000224 bp) in 2.272 CPU sec, 2.644 real sec...
[M::process] read 676668 sequences (100000134 bp) in 1.653 CPU sec, 1.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287653, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.78, 110.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676856 reads in 219.997 CPU sec, 7.100 real sec
sorting block 52, #reads = 1082008 mem = 536877941 peak = 846868480
[M::mem_process_seqs] Processed 676840 reads in 123.932 CPU sec, 3.980 real sec
sorting block 53, #reads = 1082101 mem = 536882051 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287761, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (228.46, 112.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677162 sequences (100000237 bp) in 2.395 CPU sec, 2.754 real sec...
[M::process] read 676968 sequences (100000073 bp) in 1.543 CPU sec, 1.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288095, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.94, 113.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 34, 178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (63.22, 55.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 628)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676958 reads in 214.984 CPU sec, 6.942 real sec
[M::mem_process_seqs] Processed 676668 reads in 117.435 CPU sec, 3.773 real sec
sorting block 54, #reads = 1080229 mem = 536884956 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287717, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (228.77, 111.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677194 sequences (100000137 bp) in 2.168 CPU sec, 2.579 real sec...
[M::process] read 676932 sequences (100000281 bp) in 1.597 CPU sec, 1.614 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287440, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.55, 114.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677162 reads in 217.275 CPU sec, 7.051 real sec
[M::mem_process_seqs] Processed 676968 reads in 121.784 CPU sec, 3.921 real sec
sorting block 55, #reads = 1080667 mem = 536879873 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288212, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.35, 108.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676942 sequences (100000020 bp) in 2.445 CPU sec, 2.723 real sec...
[M::process] read 676832 sequences (100000116 bp) in 1.626 CPU sec, 1.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288342, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.19, 108.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677194 reads in 207.956 CPU sec, 6.705 real sec
[M::mem_process_seqs] Processed 676932 reads in 113.849 CPU sec, 3.666 real sec
sorting block 56, #reads = 1081866 mem = 536878296 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288198, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.89, 111.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677040 sequences (100000135 bp) in 2.328 CPU sec, 2.798 real sec...
sorting block 57, #reads = 1082183 mem = 536882136 peak = 846868480
[M::process] read 676978 sequences (100000059 bp) in 1.490 CPU sec, 1.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287604, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.93, 108.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676942 reads in 212.736 CPU sec, 6.880 real sec
[M::mem_process_seqs] Processed 676832 reads in 120.948 CPU sec, 3.876 real sec
sorting block 58, #reads = 1080586 mem = 536879046 peak = 846868480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287980, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.27, 106.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676708 sequences (100000152 bp) in 2.344 CPU sec, 2.573 real sec...
[M::process] read 676846 sequences (100000085 bp) in 1.560 CPU sec, 1.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287904, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.34, 109.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677040 reads in 207.895 CPU sec, 6.672 real sec
[M::mem_process_seqs] Processed 676978 reads in 111.989 CPU sec, 3.567 real sec
sorting block 59, #reads = 1082676 mem = 536878002 peak = 846868480
[M::process] read 676706 sequences (100000064 bp) in 2.108 CPU sec, 2.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287826, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.89, 110.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676672 sequences (100000204 bp) in 1.220 CPU sec, 1.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287691, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.84, 111.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 32, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1924)
[M::mem_pestat] mean and std.dev: (258.73, 394.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2558)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676708 reads in 200.176 CPU sec, 6.423 real sec
[M::mem_process_seqs] Processed 676846 reads in 110.485 CPU sec, 3.556 real sec
sorting block 60, #reads = 1082825 mem = 536878758 peak = 846868480
[M::process] read 313930 sequences (46404761 bp) in 1.067 CPU sec, 1.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287846, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.69, 113.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 58, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488)
[M::mem_pestat] mean and std.dev: (138.22, 208.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1979)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287645, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.72, 115.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 133590, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.83, 115.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676706 reads in 245.588 CPU sec, 7.963 real sec
[M::mem_process_seqs] Processed 676672 reads in 155.694 CPU sec, 5.045 real sec
[M::mem_process_seqs] Processed 313930 reads in 159.782 CPU sec, 5.190 real sec
sorting block 61, #reads = 1082031 mem = 536878350 peak = 846868480
sorting block 62, #reads = 1082289 mem = 536880292 peak = 846868480
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67997520
overall: 69398876160 mem 13376.734 user 1073.714 sys 505.359 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L003\tPU:L003\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.T21-D0003376_R1.fastq.gz 0003.T21-D0003376_R2.fastq.gz
[main] Real time: 505.396 sec; CPU: 14450.452 sec
sorting block 63, #reads = 367469 mem = 182422876 peak = 846868480
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2729496006
output reads: 68521977
bam_mem_sort: 64 calls 62.026 user 1.169 sys 64.105 real
bam_write: 64 calls 44.331 user 3.778 sys 76.687 real
execute: 1 calls 20.503 user 2.093 sys 529.028 real
merge_files: 1 calls 20.481 user 2.061 sys 23.213 real
parse_chunk: 1014 calls 49.235 user 15.040 sys 73.984 real
read_chunk: 2730 calls 34.376 user 15.435 sys 386.389 real
sort_block: 1 calls 27.042 user 14.378 sys 375.354 real
write_chunk: 1714 calls 20.145 user 1.677 sys 22.241 real
overall: 2050220032 mem 789.677 user 64.176 sys 529.048 real
ls: cannot access '*.cram': No such file or directory