Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e9/1508dddb8e3f5c1d59fe836e121723/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/97/7870e9779bacdde37039d765313caa/T18-D0003255/0002.T18-D0003255_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/97/7870e9779bacdde37039d765313caa/T18-D0003255/0002.T18-D0003255_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e9/1508dddb8e3f5c1d59fe836e121723/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2605.060 CPU sec, 101.970 real sec
[M::process] read 673488 sequences (100000176 bp) in 0.945 CPU sec, 0.952 real sec...
[M::process] read 673386 sequences (100000163 bp) in 1.279 CPU sec, 1.284 real sec...
[M::process] read 673320 sequences (100000147 bp) in 1.149 CPU sec, 1.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286172, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (260.60, 129.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 3, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256)
[M::mem_pestat] mean and std.dev: (19.09, 32.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 341)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673334 sequences (100000048 bp) in 1.080 CPU sec, 1.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286094, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.78, 133.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286363, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (266.26, 133.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 71, 122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324)
[M::mem_pestat] mean and std.dev: (65.00, 45.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 425)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673488 reads in 298.431 CPU sec, 9.698 real sec
[M::mem_process_seqs] Processed 673386 reads in 205.630 CPU sec, 6.801 real sec
[M::mem_process_seqs] Processed 673320 reads in 219.447 CPU sec, 7.291 real sec
sorting block 0, #reads = 1080262 mem = 536880186 peak = 583774208
[M::process] read 673074 sequences (100000027 bp) in 1.412 CPU sec, 1.507 real sec...
[M::process] read 673230 sequences (100000003 bp) in 1.246 CPU sec, 1.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285681, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (266.62, 133.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673408 sequences (100000061 bp) in 1.049 CPU sec, 1.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285459, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.69, 136.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284803, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.96, 138.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 158, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116)
[M::mem_pestat] mean and std.dev: (206.55, 205.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1482)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673334 reads in 288.396 CPU sec, 9.287 real sec
sorting block 1, #reads = 1079404 mem = 536879860 peak = 838295552
[M::mem_process_seqs] Processed 673074 reads in 210.575 CPU sec, 6.836 real sec
[M::mem_process_seqs] Processed 673230 reads in 225.412 CPU sec, 7.370 real sec
sorting block 2, #reads = 1079468 mem = 536877473 peak = 838295552
[M::process] read 673390 sequences (100000176 bp) in 1.806 CPU sec, 1.932 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284776, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 673120 sequences (100000160 bp) in 1.383 CPU sec, 1.444 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.04, 135.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673234 sequences (100000200 bp) in 1.030 CPU sec, 1.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284474, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.45, 135.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 36, 117)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329)
[M::mem_pestat] mean and std.dev: (53.33, 44.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 435)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673408 reads in 204.565 CPU sec, 6.570 real sec
[M::mem_process_seqs] Processed 673390 reads in 114.205 CPU sec, 3.694 real sec
sorting block 3, #reads = 1079075 mem = 536881184 peak = 838295552
sorting block 4, #reads = 1079009 mem = 536879453 peak = 838295552
[M::process] read 673164 sequences (100000064 bp) in 1.653 CPU sec, 1.825 real sec...
[M::process] read 673058 sequences (100000055 bp) in 0.990 CPU sec, 0.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284395, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.05, 137.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (49, 86, 553)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561)
[M::mem_pestat] mean and std.dev: (213.75, 329.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2065)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285179, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.28, 139.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284309, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.77, 140.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673120 reads in 296.261 CPU sec, 9.548 real sec
[M::mem_process_seqs] Processed 673234 reads in 203.839 CPU sec, 6.633 real sec
[M::mem_process_seqs] Processed 673164 reads in 211.588 CPU sec, 6.909 real sec
sorting block 5, #reads = 1079731 mem = 536878870 peak = 838295552
[M::process] read 672920 sequences (100000079 bp) in 1.539 CPU sec, 1.680 real sec...
[M::process] read 672896 sequences (100000129 bp) in 1.363 CPU sec, 1.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284240, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.77, 143.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672764 sequences (100000145 bp) in 0.990 CPU sec, 0.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284368, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.83, 145.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 188, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1629)
[M::mem_pestat] mean and std.dev: (298.77, 281.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2166)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284850, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (274.94, 144.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673058 reads in 298.977 CPU sec, 9.629 real sec
sorting block 6, #reads = 1078740 mem = 536879153 peak = 843161600
[M::mem_process_seqs] Processed 672896 reads in 205.520 CPU sec, 6.663 real sec
[M::mem_process_seqs] Processed 672920 reads in 212.731 CPU sec, 6.920 real sec
sorting block 7, #reads = 1078680 mem = 536879238 peak = 959344640
[M::process] read 672586 sequences (100000200 bp) in 1.723 CPU sec, 1.918 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284076, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.93, 147.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673356 sequences (100000064 bp) in 1.385 CPU sec, 1.467 real sec...
[M::process] read 673344 sequences (100000041 bp) in 1.060 CPU sec, 1.059 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284977, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (285.33, 149.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 238, 860)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2534)
[M::mem_pestat] mean and std.dev: (523.44, 642.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3371)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672764 reads in 204.532 CPU sec, 6.579 real sec
[M::mem_process_seqs] Processed 672586 reads in 109.251 CPU sec, 3.534 real sec
sorting block 8, #reads = 1078996 mem = 536879287 peak = 959344640
sorting block 9, #reads = 1079498 mem = 536879447 peak = 959344640
[M::process] read 673218 sequences (100000117 bp) in 1.660 CPU sec, 1.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285769, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.94, 133.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673256 sequences (100000066 bp) in 1.030 CPU sec, 1.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285746, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.30, 135.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 75, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (208.82, 225.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1346)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285747, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.42, 138.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (92, 228, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1562)
[M::mem_pestat] mean and std.dev: (257.91, 209.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2052)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673356 reads in 276.241 CPU sec, 8.906 real sec
[M::mem_process_seqs] Processed 673344 reads in 192.117 CPU sec, 6.237 real sec
[M::mem_process_seqs] Processed 673218 reads in 199.473 CPU sec, 6.500 real sec
sorting block 10, #reads = 1079104 mem = 536879753 peak = 959344640
[M::process] read 673004 sequences (100000233 bp) in 1.665 CPU sec, 1.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285294, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.42, 137.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672988 sequences (100000012 bp) in 1.437 CPU sec, 1.540 real sec...
[M::process] read 673204 sequences (100000043 bp) in 1.050 CPU sec, 1.056 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285539, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.26, 140.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 122, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (110.50, 90.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673256 reads in 197.340 CPU sec, 6.350 real sec
sorting block 11, #reads = 1080001 mem = 536878604 peak = 959344640
[M::mem_process_seqs] Processed 673004 reads in 108.517 CPU sec, 3.511 real sec
sorting block 12, #reads = 1078995 mem = 536880415 peak = 959344640
[M::process] read 673228 sequences (100000115 bp) in 1.720 CPU sec, 1.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285309, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.59, 144.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673078 sequences (100000232 bp) in 1.030 CPU sec, 1.037 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285825, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.50, 138.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 271, 1090)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3186)
[M::mem_pestat] mean and std.dev: (483.76, 647.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4234)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285215, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.18, 138.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672988 reads in 297.283 CPU sec, 9.581 real sec
[M::mem_process_seqs] Processed 673204 reads in 208.621 CPU sec, 6.761 real sec
[M::mem_process_seqs] Processed 673228 reads in 217.604 CPU sec, 7.086 real sec
sorting block 13, #reads = 1079726 mem = 536879179 peak = 959344640
[M::process] read 673194 sequences (100000076 bp) in 2.767 CPU sec, 3.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285293, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.62, 140.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 73, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (149.62, 196.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 14, #reads = 1079911 mem = 536879767 peak = 959344640
[M::process] read 673078 sequences (100000067 bp) in 1.525 CPU sec, 1.643 real sec...
[M::process] read 673118 sequences (100000227 bp) in 1.080 CPU sec, 1.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285264, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.82, 142.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 20, 651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1927)
[M::mem_pestat] mean and std.dev: (236.55, 358.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2565)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673078 reads in 210.314 CPU sec, 6.775 real sec
[M::mem_process_seqs] Processed 673194 reads in 120.419 CPU sec, 3.876 real sec
sorting block 15, #reads = 1079689 mem = 536880269 peak = 959344640
[M::process] read 673022 sequences (100000227 bp) in 2.012 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285658, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.20, 142.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 8, 11)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25)
[M::mem_pestat] mean and std.dev: (6.62, 2.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 32)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672912 sequences (100000022 bp) in 1.019 CPU sec, 1.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285350, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (275.96, 144.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 79, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (147.50, 224.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673078 reads in 203.615 CPU sec, 6.556 real sec
[M::process] read 672754 sequences (100000280 bp) in 1.159 CPU sec, 1.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285012, 22, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.29, 144.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 71, 698)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2088)
[M::mem_pestat] mean and std.dev: (268.32, 308.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2783)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673022 reads in 114.172 CPU sec, 3.707 real sec
sorting block 16, #reads = 1079760 mem = 536877914 peak = 959344640
[M::mem_process_seqs] Processed 673118 reads in 273.190 CPU sec, 8.862 real sec
sorting block 17, #reads = 1079409 mem = 536877901 peak = 959344640
[M::process] read 672692 sequences (100000288 bp) in 2.134 CPU sec, 2.369 real sec...
[M::process] read 672960 sequences (100000169 bp) in 1.260 CPU sec, 1.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284898, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.73, 148.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 86, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278)
[M::mem_pestat] mean and std.dev: (153.38, 212.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1697)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672754 reads in 110.437 CPU sec, 3.540 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285470, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.02, 147.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 24, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284)
[M::mem_pestat] mean and std.dev: (52.20, 68.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 375)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673236 sequences (100000180 bp) in 1.163 CPU sec, 1.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285148, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (286.21, 150.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672912 reads in 327.786 CPU sec, 10.596 real sec
[M::mem_process_seqs] Processed 672692 reads in 129.961 CPU sec, 4.288 real sec
sorting block 18, #reads = 1079851 mem = 536878882 peak = 959344640
sorting block 19, #reads = 1079297 mem = 536880124 peak = 959344640
[M::process] read 673094 sequences (100000035 bp) in 2.099 CPU sec, 2.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286073, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.18, 143.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673036 sequences (100000276 bp) in 1.170 CPU sec, 1.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286701, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.72, 134.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 171, 572)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1690)
[M::mem_pestat] mean and std.dev: (213.81, 251.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2249)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672960 reads in 193.616 CPU sec, 6.226 real sec
[M::mem_process_seqs] Processed 673236 reads in 109.191 CPU sec, 3.534 real sec
sorting block 20, #reads = 1079865 mem = 536880343 peak = 959344640
[M::process] read 672940 sequences (100000177 bp) in 1.728 CPU sec, 1.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286278, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.08, 137.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 346, 962)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2714)
[M::mem_pestat] mean and std.dev: (426.67, 372.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3590)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672924 sequences (100000082 bp) in 1.080 CPU sec, 1.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285727, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.72, 140.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286120, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (272.80, 139.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 198, 675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1953)
[M::mem_pestat] mean and std.dev: (269.92, 280.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2592)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673094 reads in 281.587 CPU sec, 9.067 real sec
[M::mem_process_seqs] Processed 672940 reads in 198.760 CPU sec, 6.452 real sec
[M::mem_process_seqs] Processed 673036 reads in 208.009 CPU sec, 6.783 real sec
sorting block 21, #reads = 1079732 mem = 536878943 peak = 959344640
[M::process] read 673190 sequences (100000054 bp) in 2.149 CPU sec, 2.422 real sec...
sorting block 22, #reads = 1079513 mem = 536878281 peak = 959344640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285136, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.15, 144.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 165, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2256)
[M::mem_pestat] mean and std.dev: (397.19, 492.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2997)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673274 sequences (100000085 bp) in 1.412 CPU sec, 1.465 real sec...
[M::process] read 673092 sequences (100000014 bp) in 1.100 CPU sec, 1.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285129, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (274.39, 142.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (67, 86, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (137.33, 99.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672924 reads in 202.878 CPU sec, 6.535 real sec
[M::mem_process_seqs] Processed 673190 reads in 112.095 CPU sec, 3.606 real sec
sorting block 23, #reads = 1079595 mem = 536877780 peak = 959344640
[M::process] read 672984 sequences (100000117 bp) in 2.026 CPU sec, 2.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286155, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.19, 136.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (67, 369, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (170.00, 160.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672990 sequences (100000236 bp) in 1.110 CPU sec, 1.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285545, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.07, 139.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (82, 668, 990)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2806)
[M::mem_pestat] mean and std.dev: (534.14, 418.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3714)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285139, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.23, 142.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673274 reads in 289.394 CPU sec, 9.304 real sec
[M::mem_process_seqs] Processed 673092 reads in 200.183 CPU sec, 6.479 real sec
[M::mem_process_seqs] Processed 672984 reads in 207.682 CPU sec, 6.745 real sec
sorting block 24, #reads = 1079895 mem = 536879115 peak = 959344640
[M::process] read 673080 sequences (100000153 bp) in 1.732 CPU sec, 1.919 real sec...
sorting block 25, #reads = 1079744 mem = 536877419 peak = 959344640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285501, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.18, 143.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (386, 386, 866)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1826)
[M::mem_pestat] mean and std.dev: (445.77, 276.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2306)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673014 sequences (100000290 bp) in 2.036 CPU sec, 2.302 real sec...
[M::process] read 672840 sequences (100000232 bp) in 1.500 CPU sec, 1.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285488, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.24, 144.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 43, 1112)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3316)
[M::mem_pestat] mean and std.dev: (281.69, 455.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4418)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672990 reads in 204.530 CPU sec, 6.548 real sec
[M::mem_process_seqs] Processed 673080 reads in 111.070 CPU sec, 3.554 real sec
sorting block 26, #reads = 1079785 mem = 536879716 peak = 959344640
[M::process] read 672664 sequences (100000208 bp) in 2.027 CPU sec, 2.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283073, 21, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (275.69, 145.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 130, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1188)
[M::mem_pestat] mean and std.dev: (182.11, 251.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1582)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672636 sequences (100000242 bp) in 1.076 CPU sec, 1.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284937, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (281.92, 149.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 17, 94)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276)
[M::mem_pestat] mean and std.dev: (45.67, 73.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 367)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284896, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.41, 148.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 174, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2186)
[M::mem_pestat] mean and std.dev: (242.00, 286.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2913)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673014 reads in 292.077 CPU sec, 9.397 real sec
[M::mem_process_seqs] Processed 672840 reads in 203.028 CPU sec, 6.556 real sec
[M::mem_process_seqs] Processed 672664 reads in 210.330 CPU sec, 6.816 real sec
sorting block 27, #reads = 1076253 mem = 536877955 peak = 959344640
sorting block 28, #reads = 1079539 mem = 536881314 peak = 959344640
[M::process] read 672650 sequences (100000115 bp) in 1.811 CPU sec, 2.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285224, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.70, 149.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 234, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160)
[M::mem_pestat] mean and std.dev: (194.93, 135.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1518)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673346 sequences (100000186 bp) in 1.664 CPU sec, 1.766 real sec...
[M::process] read 673166 sequences (100000001 bp) in 1.120 CPU sec, 1.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285216, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 204, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (281.07, 145.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672636 reads in 199.048 CPU sec, 6.396 real sec
[M::mem_process_seqs] Processed 672650 reads in 108.640 CPU sec, 3.504 real sec
sorting block 29, #reads = 1079530 mem = 536881826 peak = 959344640
[M::process] read 673124 sequences (100000250 bp) in 2.236 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286223, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.83, 133.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 54, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 211)
[M::mem_pestat] mean and std.dev: (50.67, 57.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 280)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672974 sequences (100000296 bp) in 1.180 CPU sec, 1.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285483, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (268.01, 135.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285639, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.38, 137.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 74, 79)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 137)
[M::mem_pestat] mean and std.dev: (62.36, 12.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 166)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673346 reads in 285.918 CPU sec, 9.200 real sec
[M::mem_process_seqs] Processed 673166 reads in 199.919 CPU sec, 6.463 real sec
sorting block 30, #reads = 1079251 mem = 536881488 peak = 959344640
[M::mem_process_seqs] Processed 673124 reads in 208.557 CPU sec, 6.773 real sec
sorting block 31, #reads = 1079304 mem = 536879183 peak = 959344640
[M::process] read 672922 sequences (100000171 bp) in 1.992 CPU sec, 2.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285628, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.31, 138.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672852 sequences (100000054 bp) in 1.742 CPU sec, 1.879 real sec...
[M::process] read 673414 sequences (100000226 bp) in 1.190 CPU sec, 1.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284901, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.83, 141.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672974 reads in 203.370 CPU sec, 6.530 real sec
[M::mem_process_seqs] Processed 672922 reads in 112.698 CPU sec, 3.613 real sec
sorting block 32, #reads = 1079738 mem = 536878819 peak = 959344640
[M::process] read 673194 sequences (100000273 bp) in 1.690 CPU sec, 1.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284567, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.76, 143.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672920 sequences (100000132 bp) in 1.090 CPU sec, 1.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285667, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.84, 134.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285062, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.73, 138.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 92, 1419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4191)
[M::mem_pestat] mean and std.dev: (464.79, 610.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5577)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672852 reads in 291.569 CPU sec, 9.382 real sec
sorting block 33, #reads = 1079579 mem = 536879700 peak = 959344640
[M::mem_process_seqs] Processed 673414 reads in 202.100 CPU sec, 6.545 real sec
[M::mem_process_seqs] Processed 673194 reads in 209.465 CPU sec, 6.808 real sec
sorting block 34, #reads = 1079473 mem = 536876685 peak = 959344640
[M::process] read 673016 sequences (100000158 bp) in 2.773 CPU sec, 3.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284580, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.67, 139.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672974 sequences (100000213 bp) in 1.515 CPU sec, 1.602 real sec...
[M::process] read 672954 sequences (100000139 bp) in 1.370 CPU sec, 1.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285386, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.11, 140.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 655, 9729)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28891)
[M::mem_pestat] mean and std.dev: (3016.91, 4131.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38472)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672920 reads in 210.466 CPU sec, 6.726 real sec
[M::mem_process_seqs] Processed 673016 reads in 115.050 CPU sec, 3.657 real sec
sorting block 35, #reads = 1079868 mem = 536880862 peak = 959344640
sorting block 36, #reads = 1079443 mem = 536878739 peak = 959344640
[M::process] read 673006 sequences (100000084 bp) in 2.122 CPU sec, 2.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285254, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.97, 141.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672750 sequences (100000202 bp) in 1.130 CPU sec, 1.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285029, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.58, 145.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284697, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (275.66, 143.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 129, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1392)
[M::mem_pestat] mean and std.dev: (150.14, 180.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1854)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672974 reads in 299.453 CPU sec, 9.622 real sec
[M::mem_process_seqs] Processed 673006 reads in 207.165 CPU sec, 6.702 real sec
[M::mem_process_seqs] Processed 672954 reads in 215.540 CPU sec, 7.002 real sec
sorting block 37, #reads = 1079919 mem = 536880977 peak = 959344640
[M::process] read 672724 sequences (100000094 bp) in 2.254 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285017, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.45, 147.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672642 sequences (100000017 bp) in 1.706 CPU sec, 1.738 real sec...
[M::process] read 673516 sequences (100000050 bp) in 1.230 CPU sec, 1.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285429, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.53, 147.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672750 reads in 208.472 CPU sec, 6.689 real sec
sorting block 38, #reads = 1079886 mem = 536879698 peak = 959344640
[M::mem_process_seqs] Processed 672724 reads in 118.363 CPU sec, 3.798 real sec
[M::process] read 673580 sequences (100000157 bp) in 2.483 CPU sec, 2.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285334, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (285.21, 148.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 86, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125)
[M::mem_pestat] mean and std.dev: (178.50, 222.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1494)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 39, #reads = 1079377 mem = 536877767 peak = 959344640
[M::process] read 673324 sequences (100000166 bp) in 1.406 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284397, 3, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 228, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212)
[M::mem_pestat] mean and std.dev: (339.91, 163.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1540)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.53, 140.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 672642 reads in 210.367 CPU sec, 6.743 real sec
[M::mem_process_seqs] Processed 673516 reads in 120.874 CPU sec, 3.904 real sec
sorting block 40, #reads = 1079579 mem = 536877149 peak = 959344640
[M::process] read 673370 sequences (100000214 bp) in 2.050 CPU sec, 2.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285142, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.43, 139.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673260 sequences (100000075 bp) in 1.500 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284902, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.03, 141.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284477, 14, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.06, 139.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 91, 173)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (102.92, 108.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 635)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673580 reads in 304.542 CPU sec, 9.775 real sec
[M::mem_process_seqs] Processed 673370 reads in 209.801 CPU sec, 6.775 real sec
[M::mem_process_seqs] Processed 673324 reads in 217.357 CPU sec, 7.042 real sec
sorting block 41, #reads = 1079546 mem = 536878596 peak = 959344640
[M::process] read 673366 sequences (100000073 bp) in 1.997 CPU sec, 2.201 real sec...
sorting block 42, #reads = 1078978 mem = 536879752 peak = 959344640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284431, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.71, 141.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673486 sequences (100000227 bp) in 1.424 CPU sec, 1.492 real sec...
[M::process] read 673364 sequences (100000074 bp) in 1.120 CPU sec, 1.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284414, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.82, 136.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673260 reads in 208.883 CPU sec, 6.714 real sec
[M::mem_process_seqs] Processed 673366 reads in 112.598 CPU sec, 3.620 real sec
sorting block 43, #reads = 1079302 mem = 536880317 peak = 959344640
[M::process] read 673414 sequences (100000101 bp) in 1.934 CPU sec, 2.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284529, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.70, 134.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673412 sequences (100000201 bp) in 1.190 CPU sec, 1.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284374, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.68, 135.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284702, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.68, 137.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 73, 561)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1647)
[M::mem_pestat] mean and std.dev: (260.07, 273.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2190)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673486 reads in 298.442 CPU sec, 9.593 real sec
[M::mem_process_seqs] Processed 673364 reads in 207.434 CPU sec, 6.701 real sec
sorting block 44, #reads = 1079970 mem = 536878313 peak = 959344640
[M::mem_process_seqs] Processed 673414 reads in 215.898 CPU sec, 7.003 real sec
sorting block 45, #reads = 1079862 mem = 536877106 peak = 959344640
[M::process] read 673466 sequences (100000050 bp) in 1.953 CPU sec, 2.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284217, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.79, 138.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 54, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (88.60, 84.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 745)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673278 sequences (100000179 bp) in 1.551 CPU sec, 1.666 real sec...
[M::process] read 673162 sequences (100000042 bp) in 1.110 CPU sec, 1.114 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284466, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.53, 134.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673412 reads in 208.008 CPU sec, 6.662 real sec
[M::mem_process_seqs] Processed 673466 reads in 114.472 CPU sec, 3.679 real sec
sorting block 46, #reads = 1080043 mem = 536878428 peak = 959344640
[M::process] read 673128 sequences (100000251 bp) in 2.180 CPU sec, 2.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284563, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.42, 141.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 292, 603)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1757)
[M::mem_pestat] mean and std.dev: (309.00, 282.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2334)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672800 sequences (100000102 bp) in 1.540 CPU sec, 1.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283904, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.15, 143.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 165, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098)
[M::mem_pestat] mean and std.dev: (200.00, 199.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1453)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284258, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.86, 144.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673278 reads in 294.101 CPU sec, 9.428 real sec
[M::mem_process_seqs] Processed 673162 reads in 204.197 CPU sec, 6.580 real sec
sorting block 47, #reads = 1079640 mem = 536880143 peak = 959344640
[M::mem_process_seqs] Processed 673128 reads in 212.384 CPU sec, 6.873 real sec
sorting block 48, #reads = 1079648 mem = 536878787 peak = 959344640
[M::process] read 672970 sequences (100000296 bp) in 2.114 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284128, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (277.66, 147.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 12, 42)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 120)
[M::mem_pestat] mean and std.dev: (9.83, 11.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 159)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673634 sequences (100000241 bp) in 1.340 CPU sec, 1.376 real sec...
[M::process] read 673518 sequences (100000209 bp) in 1.140 CPU sec, 1.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284026, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (280.42, 150.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 1841, 9729)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28909)
[M::mem_pestat] mean and std.dev: (3223.88, 3858.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38499)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672800 reads in 204.750 CPU sec, 6.551 real sec
[M::mem_process_seqs] Processed 672970 reads in 112.148 CPU sec, 3.604 real sec
sorting block 49, #reads = 1079557 mem = 536876493 peak = 959344640
[M::process] read 673388 sequences (100000050 bp) in 2.126 CPU sec, 2.255 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285531, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.82, 138.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 130, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (163.31, 122.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673298 sequences (100000133 bp) in 1.140 CPU sec, 1.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284605, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (266.67, 139.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 15, 88)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238)
[M::mem_pestat] mean and std.dev: (33.00, 36.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 313)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285084, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.76, 141.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673634 reads in 286.306 CPU sec, 9.189 real sec
sorting block 50, #reads = 1078863 mem = 536878886 peak = 959344640
[M::mem_process_seqs] Processed 673518 reads in 198.087 CPU sec, 6.395 real sec
[M::mem_process_seqs] Processed 673388 reads in 205.770 CPU sec, 6.668 real sec
sorting block 51, #reads = 1079770 mem = 536877694 peak = 959344640
[M::process] read 673256 sequences (100000109 bp) in 1.845 CPU sec, 2.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284935, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.59, 143.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673344 sequences (100000034 bp) in 1.882 CPU sec, 2.031 real sec...
[M::process] read 673448 sequences (100000019 bp) in 1.520 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284533, 15, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.94, 145.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 124, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (128.33, 59.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673298 reads in 200.524 CPU sec, 6.401 real sec
[M::mem_process_seqs] Processed 673256 reads in 111.519 CPU sec, 3.555 real sec
sorting block 52, #reads = 1079660 mem = 536879456 peak = 959344640
sorting block 53, #reads = 1079593 mem = 536879719 peak = 959344640
[M::process] read 673462 sequences (100000151 bp) in 2.144 CPU sec, 2.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284934, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.77, 141.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (65, 98, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1634)
[M::mem_pestat] mean and std.dev: (155.27, 173.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2157)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673502 sequences (100000228 bp) in 1.490 CPU sec, 1.486 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285468, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.61, 135.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284818, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.23, 138.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673344 reads in 285.558 CPU sec, 9.153 real sec
[M::mem_process_seqs] Processed 673448 reads in 199.106 CPU sec, 6.418 real sec
[M::mem_process_seqs] Processed 673462 reads in 207.791 CPU sec, 6.728 real sec
sorting block 54, #reads = 1080154 mem = 536878461 peak = 959344640
[M::process] read 673418 sequences (100000142 bp) in 2.186 CPU sec, 2.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284891, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.03, 139.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673242 sequences (100000001 bp) in 1.596 CPU sec, 1.703 real sec...
[M::process] read 673224 sequences (100000130 bp) in 1.140 CPU sec, 1.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283798, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.80, 139.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673502 reads in 202.213 CPU sec, 6.486 real sec
sorting block 55, #reads = 1080162 mem = 536882625 peak = 959344640
[M::mem_process_seqs] Processed 673418 reads in 110.480 CPU sec, 3.536 real sec
sorting block 56, #reads = 1080036 mem = 536877024 peak = 959344640
[M::process] read 673238 sequences (100000234 bp) in 1.901 CPU sec, 2.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281147, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.07, 141.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 47, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364)
[M::mem_pestat] mean and std.dev: (76.70, 100.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 481)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672984 sequences (100000287 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284213, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.45, 141.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284654, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.46, 146.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673242 reads in 308.021 CPU sec, 9.905 real sec
[M::mem_process_seqs] Processed 673224 reads in 223.969 CPU sec, 7.256 real sec
[M::mem_process_seqs] Processed 673238 reads in 233.041 CPU sec, 7.587 real sec
sorting block 57, #reads = 1074142 mem = 536877845 peak = 959344640
[M::process] read 673028 sequences (100000085 bp) in 2.476 CPU sec, 2.761 real sec...
sorting block 58, #reads = 1079862 mem = 536876720 peak = 959344640
[M::process] read 673460 sequences (100000165 bp) in 2.029 CPU sec, 2.138 real sec...
[M::process] read 673418 sequences (100000107 bp) in 1.570 CPU sec, 1.568 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284251, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (279.09, 151.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 86, 689)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1895)
[M::mem_pestat] mean and std.dev: (239.27, 273.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2498)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673028 reads in 128.739 CPU sec, 4.094 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283658, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (277.36, 149.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673364 sequences (100000105 bp) in 1.653 CPU sec, 1.658 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285126, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.20, 144.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672984 reads in 366.612 CPU sec, 11.720 real sec
[M::mem_process_seqs] Processed 673460 reads in 135.675 CPU sec, 4.377 real sec
sorting block 59, #reads = 1079405 mem = 536879325 peak = 959344640
[M::process] read 673202 sequences (100000062 bp) in 2.282 CPU sec, 2.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285630, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (269.63, 141.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 30, 188)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558)
[M::mem_pestat] mean and std.dev: (44.00, 60.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673194 sequences (100000258 bp) in 1.520 CPU sec, 1.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285498, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (273.98, 144.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285430, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.27, 143.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673418 reads in 335.026 CPU sec, 10.747 real sec
sorting block 60, #reads = 1078807 mem = 536880084 peak = 959344640
[M::mem_process_seqs] Processed 673202 reads in 231.826 CPU sec, 7.483 real sec
[M::mem_process_seqs] Processed 673364 reads in 240.358 CPU sec, 7.783 real sec
sorting block 61, #reads = 1079744 mem = 536880212 peak = 959344640
[M::process] read 673026 sequences (100000276 bp) in 2.393 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285110, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.61, 146.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 30, 68)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 168)
[M::mem_pestat] mean and std.dev: (26.62, 16.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 218)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673370 sequences (100000007 bp) in 2.250 CPU sec, 2.407 real sec...
[M::process] read 673386 sequences (100000225 bp) in 1.590 CPU sec, 1.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284414, 27, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (277.89, 148.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 23, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252)
[M::mem_pestat] mean and std.dev: (44.00, 56.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 335)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673194 reads in 239.001 CPU sec, 7.649 real sec
[M::mem_process_seqs] Processed 673026 reads in 130.655 CPU sec, 4.159 real sec
sorting block 62, #reads = 1079761 mem = 536882240 peak = 959344640
sorting block 63, #reads = 1079888 mem = 536878600 peak = 959344640
[M::process] read 673320 sequences (100000062 bp) in 2.540 CPU sec, 2.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285046, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.95, 136.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 94, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (83.00, 84.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673386 sequences (100000231 bp) in 1.580 CPU sec, 1.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285581, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.07, 134.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284503, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.02, 138.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673370 reads in 333.028 CPU sec, 10.682 real sec
[M::mem_process_seqs] Processed 673386 reads in 232.073 CPU sec, 7.467 real sec
[M::mem_process_seqs] Processed 673320 reads in 240.538 CPU sec, 7.764 real sec
sorting block 64, #reads = 1079470 mem = 536880407 peak = 959344640
[M::process] read 673160 sequences (100000076 bp) in 2.550 CPU sec, 2.806 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284827, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (268.10, 140.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673196 sequences (100000105 bp) in 2.124 CPU sec, 2.246 real sec...
[M::process] read 673160 sequences (100000128 bp) in 1.580 CPU sec, 1.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284894, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (270.45, 143.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673386 reads in 234.620 CPU sec, 7.491 real sec
sorting block 65, #reads = 1079772 mem = 536878238 peak = 959344640
[M::mem_process_seqs] Processed 673160 reads in 125.756 CPU sec, 4.000 real sec
sorting block 66, #reads = 1078906 mem = 536879157 peak = 959344640
[M::process] read 673030 sequences (100000198 bp) in 2.518 CPU sec, 2.721 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284984, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.14, 143.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 102, 2342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7006)
[M::mem_pestat] mean and std.dev: (842.08, 1032.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9338)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673066 sequences (100000075 bp) in 1.590 CPU sec, 1.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284369, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.48, 144.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284771, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (274.94, 146.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (93, 241, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1761)
[M::mem_pestat] mean and std.dev: (438.91, 407.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2317)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673196 reads in 348.023 CPU sec, 11.163 real sec
[M::mem_process_seqs] Processed 673160 reads in 239.213 CPU sec, 7.710 real sec
[M::mem_process_seqs] Processed 673030 reads in 248.203 CPU sec, 8.027 real sec
sorting block 67, #reads = 1079274 mem = 536878747 peak = 959344640
[M::process] read 672908 sequences (100000126 bp) in 1.966 CPU sec, 2.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284804, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (277.33, 148.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1079715 mem = 536877374 peak = 959344640
[M::process] read 673502 sequences (100000016 bp) in 2.353 CPU sec, 2.567 real sec...
[M::process] read 673370 sequences (100000167 bp) in 1.580 CPU sec, 1.577 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284780, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (279.82, 151.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (65, 86, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (121.64, 110.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673066 reads in 238.964 CPU sec, 7.640 real sec
[M::mem_process_seqs] Processed 672908 reads in 128.901 CPU sec, 4.109 real sec
sorting block 69, #reads = 1079329 mem = 536878944 peak = 959344640
[M::process] read 673294 sequences (100000104 bp) in 2.167 CPU sec, 2.299 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285597, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (269.62, 143.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673186 sequences (100000161 bp) in 1.550 CPU sec, 1.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285311, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (273.97, 145.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284373, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.45, 146.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673502 reads in 327.151 CPU sec, 10.501 real sec
[M::mem_process_seqs] Processed 673370 reads in 236.569 CPU sec, 7.647 real sec
[M::mem_process_seqs] Processed 673294 reads in 247.187 CPU sec, 8.025 real sec
sorting block 70, #reads = 1078952 mem = 536878153 peak = 959344640
[M::process] read 672996 sequences (100000173 bp) in 2.430 CPU sec, 2.675 real sec...
sorting block 71, #reads = 1079598 mem = 536879785 peak = 959344640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284577, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.40, 146.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673096 sequences (100000130 bp) in 2.120 CPU sec, 2.247 real sec...
[M::process] read 673418 sequences (100000233 bp) in 1.439 CPU sec, 1.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284939, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (277.30, 146.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 62, 1091)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3235)
[M::mem_pestat] mean and std.dev: (423.25, 491.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4307)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673186 reads in 242.957 CPU sec, 7.772 real sec
[M::mem_process_seqs] Processed 672996 reads in 135.880 CPU sec, 4.349 real sec
sorting block 72, #reads = 1078818 mem = 536876377 peak = 959344640
[M::process] read 673222 sequences (100000092 bp) in 2.489 CPU sec, 2.679 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284264, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (276.97, 146.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673252 sequences (100000004 bp) in 1.580 CPU sec, 1.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285421, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.23, 133.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285014, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.57, 138.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673096 reads in 334.594 CPU sec, 10.744 real sec
[M::mem_process_seqs] Processed 673418 reads in 239.781 CPU sec, 7.769 real sec
[M::mem_process_seqs] Processed 673222 reads in 255.099 CPU sec, 8.319 real sec
sorting block 73, #reads = 1078699 mem = 536879346 peak = 959344640
[M::process] read 673220 sequences (100000127 bp) in 2.836 CPU sec, 3.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284925, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.60, 140.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 74, #reads = 1079827 mem = 536877274 peak = 959344640
[M::process] read 673156 sequences (100000254 bp) in 3.078 CPU sec, 3.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284352, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.61, 140.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 48, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (170.92, 231.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1342)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673050 sequences (100000287 bp) in 1.450 CPU sec, 1.451 real sec...
[M::mem_process_seqs] Processed 673252 reads in 254.044 CPU sec, 8.167 real sec
[M::mem_process_seqs] Processed 673220 reads in 153.451 CPU sec, 4.930 real sec
sorting block 75, #reads = 1079947 mem = 536876405 peak = 959344640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284235, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.38, 143.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673098 sequences (100000246 bp) in 3.495 CPU sec, 3.914 real sec...
[M::process] read 672896 sequences (100000156 bp) in 1.246 CPU sec, 1.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284304, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.63, 144.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673156 reads in 251.344 CPU sec, 8.072 real sec
[M::mem_process_seqs] Processed 673050 reads in 148.035 CPU sec, 4.798 real sec
sorting block 76, #reads = 1079758 mem = 536879720 peak = 959344640
[M::process] read 672910 sequences (100000064 bp) in 2.528 CPU sec, 2.779 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283811, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (276.40, 146.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 555312 sequences (82538038 bp) in 1.390 CPU sec, 1.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284133, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (277.75, 148.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 350, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1758)
[M::mem_pestat] mean and std.dev: (279.00, 231.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2271)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673098 reads in 250.484 CPU sec, 8.058 real sec
sorting block 77, #reads = 1079268 mem = 536880534 peak = 959344640
[M::mem_process_seqs] Processed 672896 reads in 146.902 CPU sec, 4.768 real sec
sorting block 78, #reads = 1079566 mem = 536880236 peak = 959344640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284622, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (278.93, 148.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 234300, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (284.20, 154.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672910 reads in 216.073 CPU sec, 7.049 real sec
[M::mem_process_seqs] Processed 555312 reads in 116.270 CPU sec, 3.810 real sec
sorting block 79, #reads = 1079342 mem = 536880564 peak = 959344640
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 86045904
overall: 69569699840 mem 15502.965 user 1172.947 sys 572.272 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L001\tPU:L001\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.T18-D0003255_R1.fastq.gz 0002.T18-D0003255_R2.fastq.gz
[main] Real time: 572.302 sec; CPU: 16675.915 sec
sorting block 80, #reads = 327997 mem = 163336881 peak = 959344640
uniq header count 1
merging 81 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 3324796853
output reads: 86682105
bam_mem_sort: 81 calls 48.553 user 0.970 sys 49.647 real
bam_write: 81 calls 26.079 user 1.183 sys 27.328 real
execute: 1 calls 25.450 user 1.497 sys 600.472 real
merge_files: 1 calls 25.434 user 1.466 sys 27.899 real
parse_chunk: 1285 calls 53.897 user 13.551 sys 68.365 real
read_chunk: 3459 calls 40.428 user 26.744 sys 476.332 real
sort_block: 1 calls 30.252 user 25.897 sys 459.932 real
write_chunk: 2172 calls 24.962 user 1.218 sys 26.735 real
overall: 2335367168 mem 921.841 user 60.808 sys 600.488 real
ls: cannot access '*.cram': No such file or directory