This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2424.960 CPU sec, 97.741 real sec
[M::process] read 677680 sequences (100000145 bp) in 1.304 CPU sec, 1.305 real sec...
[M::process] read 677452 sequences (100000001 bp) in 1.541 CPU sec, 1.550 real sec...
[M::process] read 677288 sequences (100000132 bp) in 1.490 CPU sec, 1.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289026, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (215.04, 93.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677164 sequences (100000168 bp) in 1.295 CPU sec, 1.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288754, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.66, 100.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288729, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.71, 102.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 213, 2350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6642)
[M::mem_pestat] mean and std.dev: (961.45, 1051.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8788)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677680 reads in 276.456 CPU sec, 8.956 real sec
[M::mem_process_seqs] Processed 677452 reads in 178.206 CPU sec, 5.839 real sec
[M::mem_process_seqs] Processed 677288 reads in 185.614 CPU sec, 6.099 real sec
sorting block 0, #reads = 1082807 mem = 536879938 peak = 588541952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288828, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.64, 105.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676936 sequences (100000037 bp) in 2.611 CPU sec, 2.947 real sec...
[M::mem_process_seqs] Processed 677164 reads in 93.576 CPU sec, 3.072 real sec
sorting block 1, #reads = 1082850 mem = 536878683 peak = 774139904
[M::process] read 676850 sequences (100000179 bp) in 2.577 CPU sec, 2.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288575, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.99, 106.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 91, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1238)
[M::mem_pestat] mean and std.dev: (147.00, 158.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1638)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676736 sequences (100000039 bp) in 1.380 CPU sec, 1.379 real sec...
[M::process] read 676656 sequences (100000201 bp) in 1.299 CPU sec, 1.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288233, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.39, 108.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676936 reads in 184.332 CPU sec, 5.837 real sec
[M::mem_process_seqs] Processed 676850 reads in 103.933 CPU sec, 3.288 real sec
sorting block 2, #reads = 1082783 mem = 536878726 peak = 774139904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288002, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.75, 109.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676566 sequences (100000136 bp) in 2.937 CPU sec, 3.283 real sec...
[M::process] read 676488 sequences (100000209 bp) in 1.267 CPU sec, 1.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288249, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.23, 111.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676736 reads in 187.360 CPU sec, 5.975 real sec
[M::mem_process_seqs] Processed 676656 reads in 105.356 CPU sec, 3.360 real sec
sorting block 3, #reads = 1082468 mem = 536878153 peak = 774139904
sorting block 4, #reads = 1082347 mem = 536879584 peak = 785850368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288028, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.86, 113.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 315, 1320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3522)
[M::mem_pestat] mean and std.dev: (641.73, 466.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4623)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676370 sequences (100000216 bp) in 2.384 CPU sec, 2.647 real sec...
[M::process] read 676206 sequences (100000026 bp) in 1.660 CPU sec, 1.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287905, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.66, 114.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676566 reads in 190.833 CPU sec, 6.091 real sec
[M::mem_process_seqs] Processed 676488 reads in 107.603 CPU sec, 3.422 real sec
sorting block 5, #reads = 1082162 mem = 536880476 peak = 785850368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288032, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.19, 115.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676676 sequences (100000140 bp) in 2.878 CPU sec, 3.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287320, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.35, 118.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 87, 100)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286)
[M::mem_pestat] mean and std.dev: (43.62, 39.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 379)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677538 sequences (100000270 bp) in 1.560 CPU sec, 1.560 real sec...
[M::mem_process_seqs] Processed 676370 reads in 192.002 CPU sec, 6.133 real sec
[M::mem_process_seqs] Processed 676206 reads in 109.480 CPU sec, 3.466 real sec
sorting block 6, #reads = 1082103 mem = 536877791 peak = 785850368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288382, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.28, 112.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 91, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (205.20, 161.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677482 sequences (100000053 bp) in 2.890 CPU sec, 3.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288951, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.42, 98.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677142 sequences (100000174 bp) in 1.590 CPU sec, 1.596 real sec...
[M::mem_process_seqs] Processed 676676 reads in 186.708 CPU sec, 5.971 real sec
[M::mem_process_seqs] Processed 677538 reads in 107.223 CPU sec, 3.409 real sec
sorting block 7, #reads = 1081843 mem = 536879020 peak = 785850368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289102, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.66, 102.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677016 sequences (100000032 bp) in 2.783 CPU sec, 3.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288705, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.52, 105.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 364, 1772)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5248)
[M::mem_pestat] mean and std.dev: (652.83, 785.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6986)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677070 sequences (100000206 bp) in 1.690 CPU sec, 1.692 real sec...
[M::mem_process_seqs] Processed 677482 reads in 188.627 CPU sec, 6.023 real sec
[M::mem_process_seqs] Processed 677142 reads in 105.251 CPU sec, 3.339 real sec
sorting block 8, #reads = 1083164 mem = 536878505 peak = 785850368
sorting block 9, #reads = 1082656 mem = 536879048 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288056, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.51, 105.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676780 sequences (100000120 bp) in 2.392 CPU sec, 2.720 real sec...
[M::process] read 676690 sequences (100000129 bp) in 1.240 CPU sec, 1.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288088, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.60, 109.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677016 reads in 190.257 CPU sec, 6.095 real sec
[M::mem_process_seqs] Processed 677070 reads in 106.986 CPU sec, 3.416 real sec
sorting block 10, #reads = 1082582 mem = 536879473 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288538, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.16, 110.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676692 sequences (100000078 bp) in 2.868 CPU sec, 3.212 real sec...
[M::process] read 676462 sequences (100000095 bp) in 1.359 CPU sec, 1.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288016, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.22, 110.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676780 reads in 190.457 CPU sec, 6.078 real sec
[M::mem_process_seqs] Processed 676690 reads in 105.895 CPU sec, 3.367 real sec
sorting block 11, #reads = 1082627 mem = 536877966 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287600, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.85, 111.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (67, 185, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (260.91, 298.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1453)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676478 sequences (100000199 bp) in 2.460 CPU sec, 2.748 real sec...
[M::process] read 676486 sequences (100000027 bp) in 1.639 CPU sec, 1.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288161, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.95, 114.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676692 reads in 190.450 CPU sec, 6.093 real sec
[M::mem_process_seqs] Processed 676462 reads in 106.465 CPU sec, 3.382 real sec
sorting block 12, #reads = 1082510 mem = 536879018 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287932, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.64, 115.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676246 sequences (100000238 bp) in 2.866 CPU sec, 3.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287990, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.23, 116.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676478 reads in 186.300 CPU sec, 5.949 real sec
[M::mem_process_seqs] Processed 676486 reads in 106.336 CPU sec, 3.356 real sec
[M::process] read 677240 sequences (100000298 bp) in 1.982 CPU sec, 2.033 real sec...
sorting block 13, #reads = 1081805 mem = 536880175 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288067, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.51, 119.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 372, 558)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640)
[M::mem_pestat] mean and std.dev: (273.42, 256.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2181)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 14, #reads = 1082228 mem = 536878487 peak = 871129088
[M::process] read 677566 sequences (100000153 bp) in 2.642 CPU sec, 2.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288348, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.94, 107.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677256 sequences (100000131 bp) in 1.870 CPU sec, 1.873 real sec...
[M::mem_process_seqs] Processed 676246 reads in 191.333 CPU sec, 6.276 real sec
[M::mem_process_seqs] Processed 677240 reads in 110.090 CPU sec, 3.687 real sec
sorting block 15, #reads = 1081703 mem = 536878557 peak = 871129088
[M::process] read 677228 sequences (100000022 bp) in 3.521 CPU sec, 3.858 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288523, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.11, 104.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677256 reads in 98.977 CPU sec, 3.130 real sec
[M::process] read 677020 sequences (100000004 bp) in 3.038 CPU sec, 3.296 real sec...
sorting block 16, #reads = 1082462 mem = 536878057 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288825, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.88, 100.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288270, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (224.97, 105.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 24, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (95.73, 132.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677566 reads in 283.981 CPU sec, 9.079 real sec
[M::mem_process_seqs] Processed 677228 reads in 115.866 CPU sec, 3.699 real sec
[M::process] read 676908 sequences (100000020 bp) in 2.593 CPU sec, 2.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288377, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.92, 106.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1082810 mem = 536879305 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287854, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.43, 109.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 42, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (88.33, 85.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677020 reads in 186.555 CPU sec, 5.991 real sec
[M::process] read 676758 sequences (100000260 bp) in 3.614 CPU sec, 4.022 real sec...
[M::mem_process_seqs] Processed 676908 reads in 107.436 CPU sec, 3.427 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287850, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.83, 111.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 78, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (165.40, 193.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 18, #reads = 1082923 mem = 536880153 peak = 871129088
[M::mem_process_seqs] Processed 676758 reads in 98.561 CPU sec, 3.264 real sec
[M::process] read 676670 sequences (100000088 bp) in 3.159 CPU sec, 3.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288102, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.51, 113.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676670 reads in 97.603 CPU sec, 3.244 real sec
[M::process] read 676660 sequences (100000128 bp) in 3.751 CPU sec, 4.251 real sec...
sorting block 19, #reads = 1082582 mem = 536878022 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287853, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.96, 114.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676660 reads in 102.187 CPU sec, 3.388 real sec
[M::process] read 676502 sequences (100000012 bp) in 4.096 CPU sec, 4.624 real sec...
sorting block 20, #reads = 1082012 mem = 536878779 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288281, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.82, 115.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676502 reads in 101.238 CPU sec, 3.302 real sec
[M::process] read 676520 sequences (100000005 bp) in 4.593 CPU sec, 5.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288377, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.93, 115.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676520 reads in 98.346 CPU sec, 3.192 real sec
sorting block 21, #reads = 1082281 mem = 536879029 peak = 871129088
[M::process] read 676280 sequences (100000084 bp) in 4.947 CPU sec, 5.610 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288173, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.54, 119.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676280 reads in 98.506 CPU sec, 3.170 real sec
[M::process] read 676148 sequences (100000014 bp) in 4.129 CPU sec, 4.577 real sec...
sorting block 22, #reads = 1082494 mem = 536879518 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288078, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.59, 119.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676148 reads in 98.665 CPU sec, 3.185 real sec
[M::process] read 677516 sequences (100000114 bp) in 3.122 CPU sec, 3.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288800, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (218.52, 97.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677516 reads in 102.164 CPU sec, 3.315 real sec
sorting block 23, #reads = 1081869 mem = 536880229 peak = 871129088
[M::process] read 677284 sequences (100000155 bp) in 4.698 CPU sec, 5.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288239, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.65, 101.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677284 reads in 100.338 CPU sec, 3.249 real sec
[M::process] read 677272 sequences (100000239 bp) in 3.977 CPU sec, 4.457 real sec...
sorting block 24, #reads = 1082621 mem = 536879487 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288484, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (222.32, 102.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677272 reads in 101.820 CPU sec, 3.298 real sec
[M::process] read 677016 sequences (100000296 bp) in 4.088 CPU sec, 4.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288443, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (225.02, 105.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677016 reads in 106.640 CPU sec, 3.595 real sec
[M::process] read 676916 sequences (100000205 bp) in 4.077 CPU sec, 4.555 real sec...
sorting block 25, #reads = 1082315 mem = 536881999 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288364, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (226.82, 107.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676916 reads in 108.439 CPU sec, 3.519 real sec
[M::process] read 676812 sequences (100000279 bp) in 4.445 CPU sec, 5.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288090, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.85, 109.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 479, 754)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2104)
[M::mem_pestat] mean and std.dev: (380.17, 311.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2779)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676812 reads in 103.234 CPU sec, 3.343 real sec
sorting block 26, #reads = 1082454 mem = 536877418 peak = 871129088
[M::process] read 676772 sequences (100000192 bp) in 4.178 CPU sec, 4.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288079, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.36, 110.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676772 reads in 104.167 CPU sec, 3.366 real sec
[M::process] read 676688 sequences (100000237 bp) in 3.639 CPU sec, 4.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287718, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.62, 112.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (68, 379, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (242.30, 156.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676688 reads in 108.072 CPU sec, 3.563 real sec
sorting block 27, #reads = 1082281 mem = 536880419 peak = 871129088
[M::process] read 676684 sequences (100000038 bp) in 4.079 CPU sec, 4.612 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287850, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.23, 114.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (81, 91, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (92.78, 70.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 693)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676684 reads in 107.282 CPU sec, 3.533 real sec
[M::process] read 676464 sequences (100000243 bp) in 4.645 CPU sec, 5.219 real sec...
sorting block 28, #reads = 1082329 mem = 536877064 peak = 871129088
[M::process] read 676350 sequences (100000097 bp) in 2.762 CPU sec, 3.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287924, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.61, 115.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676330 sequences (100000107 bp) in 1.958 CPU sec, 2.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288232, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.62, 117.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676464 reads in 203.522 CPU sec, 6.512 real sec
[M::mem_process_seqs] Processed 676350 reads in 116.968 CPU sec, 3.719 real sec
[M::process] read 676640 sequences (100000188 bp) in 2.383 CPU sec, 2.494 real sec...
sorting block 29, #reads = 1082293 mem = 536880517 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287872, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.98, 117.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677748 sequences (100000019 bp) in 3.263 CPU sec, 3.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288082, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.47, 115.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676330 reads in 200.319 CPU sec, 6.470 real sec
[M::mem_process_seqs] Processed 676640 reads in 115.567 CPU sec, 3.694 real sec
sorting block 30, #reads = 1082207 mem = 536879387 peak = 871129088
[M::process] read 677578 sequences (100000190 bp) in 2.366 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288261, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (208.97, 85.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 621)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1081755 mem = 536876638 peak = 871129088
[M::process] read 677558 sequences (100000194 bp) in 2.204 CPU sec, 2.550 real sec...
[M::process] read 677388 sequences (100000296 bp) in 1.939 CPU sec, 1.941 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288216, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (214.53, 93.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677748 reads in 204.017 CPU sec, 6.524 real sec
[M::mem_process_seqs] Processed 677578 reads in 116.906 CPU sec, 3.727 real sec
sorting block 32, #reads = 1082827 mem = 536879608 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288510, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (217.96, 98.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677302 sequences (100000108 bp) in 3.567 CPU sec, 3.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288097, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.81, 99.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677558 reads in 208.170 CPU sec, 6.669 real sec
[M::mem_process_seqs] Processed 677388 reads in 118.847 CPU sec, 3.776 real sec
[M::process] read 677010 sequences (100000089 bp) in 2.144 CPU sec, 2.179 real sec...
sorting block 33, #reads = 1082367 mem = 536878237 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287999, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (222.19, 104.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 63, 1395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4143)
[M::mem_pestat] mean and std.dev: (608.91, 926.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5517)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677096 sequences (100000125 bp) in 2.921 CPU sec, 3.126 real sec...
[M::process] read 676878 sequences (100000108 bp) in 1.840 CPU sec, 1.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288241, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.89, 107.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677302 reads in 211.807 CPU sec, 6.792 real sec
[M::mem_process_seqs] Processed 677010 reads in 122.343 CPU sec, 3.920 real sec
sorting block 34, #reads = 1082946 mem = 536879357 peak = 871129088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287801, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.79, 109.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676780 sequences (100000102 bp) in 3.410 CPU sec, 3.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287868, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (229.08, 111.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676774 sequences (100000088 bp) in 1.916 CPU sec, 1.924 real sec...
[M::mem_process_seqs] Processed 677096 reads in 213.800 CPU sec, 6.842 real sec
sorting block 35, #reads = 1083205 mem = 536879830 peak = 871129088
[M::mem_process_seqs] Processed 676878 reads in 123.011 CPU sec, 3.918 real sec
sorting block 36, #reads = 1082660 mem = 536879202 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287356, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.80, 111.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676742 sequences (100000093 bp) in 3.109 CPU sec, 3.361 real sec...
[M::process] read 676554 sequences (100000212 bp) in 1.938 CPU sec, 1.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287992, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.00, 112.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676780 reads in 210.695 CPU sec, 6.736 real sec
[M::mem_process_seqs] Processed 676774 reads in 119.024 CPU sec, 3.799 real sec
sorting block 37, #reads = 1082531 mem = 536879266 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287895, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.61, 114.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677882 sequences (100000144 bp) in 2.591 CPU sec, 2.794 real sec...
[M::process] read 677780 sequences (100000279 bp) in 1.968 CPU sec, 1.972 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287889, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.45, 116.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676742 reads in 211.414 CPU sec, 6.776 real sec
[M::mem_process_seqs] Processed 676554 reads in 120.605 CPU sec, 3.868 real sec
sorting block 38, #reads = 1082182 mem = 536879164 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288594, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (210.03, 88.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677566 sequences (100000215 bp) in 2.818 CPU sec, 3.057 real sec...
[M::process] read 677472 sequences (100000181 bp) in 1.795 CPU sec, 1.801 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288835, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (210.24, 87.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677882 reads in 204.052 CPU sec, 6.545 real sec
[M::mem_process_seqs] Processed 677780 reads in 116.444 CPU sec, 3.732 real sec
sorting block 39, #reads = 1082269 mem = 536879770 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288728, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.52, 99.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677198 sequences (100000098 bp) in 3.424 CPU sec, 3.773 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288588, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.05, 99.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 51, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 228)
[M::mem_pestat] mean and std.dev: (42.50, 25.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 297)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677566 reads in 209.291 CPU sec, 6.701 real sec
[M::process] read 677078 sequences (100000025 bp) in 2.168 CPU sec, 2.197 real sec...
sorting block 40, #reads = 1083340 mem = 536880124 peak = 883490816
[M::mem_process_seqs] Processed 677472 reads in 123.860 CPU sec, 3.942 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288387, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.86, 103.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 92, 172)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (84.11, 77.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 41, #reads = 1082326 mem = 536880165 peak = 883490816
[M::process] read 677036 sequences (100000085 bp) in 3.038 CPU sec, 3.326 real sec...
[M::process] read 676860 sequences (100000231 bp) in 1.760 CPU sec, 1.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287932, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.59, 106.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677198 reads in 210.889 CPU sec, 6.786 real sec
[M::mem_process_seqs] Processed 677078 reads in 124.023 CPU sec, 3.973 real sec
sorting block 42, #reads = 1082110 mem = 536877496 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287379, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.82, 108.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676956 sequences (100000116 bp) in 3.583 CPU sec, 3.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287743, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.56, 110.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677036 reads in 214.882 CPU sec, 6.873 real sec
[M::process] read 676836 sequences (100000209 bp) in 1.980 CPU sec, 1.980 real sec...
[M::mem_process_seqs] Processed 676860 reads in 121.911 CPU sec, 3.881 real sec
sorting block 43, #reads = 1082549 mem = 536878181 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287994, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.83, 111.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 58, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (179.20, 251.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676794 sequences (100000044 bp) in 3.010 CPU sec, 3.290 real sec...
[M::process] read 676756 sequences (100000004 bp) in 1.840 CPU sec, 1.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287743, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.86, 112.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676956 reads in 213.187 CPU sec, 6.836 real sec
[M::mem_process_seqs] Processed 676836 reads in 125.217 CPU sec, 4.033 real sec
sorting block 44, #reads = 1082644 mem = 536879922 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288102, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.90, 112.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1082872 mem = 536878577 peak = 883490816
[M::process] read 677544 sequences (100000115 bp) in 3.899 CPU sec, 4.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287472, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.61, 114.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676794 reads in 219.128 CPU sec, 7.047 real sec
[M::mem_process_seqs] Processed 676756 reads in 126.372 CPU sec, 4.055 real sec
[M::process] read 677740 sequences (100000276 bp) in 2.153 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288269, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (217.38, 98.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1082291 mem = 536879201 peak = 883490816
[M::process] read 677534 sequences (100000214 bp) in 3.300 CPU sec, 3.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289379, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (212.01, 90.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 653)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677544 reads in 210.661 CPU sec, 6.771 real sec
[M::mem_process_seqs] Processed 677740 reads in 121.850 CPU sec, 3.885 real sec
[M::process] read 677512 sequences (100000225 bp) in 1.993 CPU sec, 2.032 real sec...
sorting block 47, #reads = 1082228 mem = 536881645 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288287, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (218.83, 99.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 41, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199)
[M::mem_pestat] mean and std.dev: (39.00, 32.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 263)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677316 sequences (100000164 bp) in 3.624 CPU sec, 3.981 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288365, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.04, 101.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677534 reads in 212.395 CPU sec, 6.849 real sec
[M::mem_process_seqs] Processed 677512 reads in 124.223 CPU sec, 3.988 real sec
[M::process] read 677278 sequences (100000032 bp) in 2.461 CPU sec, 2.617 real sec...
sorting block 48, #reads = 1082502 mem = 536879139 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288648, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (220.92, 102.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676992 sequences (100000228 bp) in 2.910 CPU sec, 3.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287922, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (224.91, 107.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677316 reads in 212.730 CPU sec, 6.874 real sec
[M::mem_process_seqs] Processed 677278 reads in 124.355 CPU sec, 4.027 real sec
[M::process] read 676992 sequences (100000189 bp) in 1.961 CPU sec, 2.041 real sec...
sorting block 49, #reads = 1083030 mem = 536878586 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288067, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.72, 108.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676782 sequences (100000270 bp) in 3.034 CPU sec, 3.645 real sec...
sorting block 50, #reads = 1082822 mem = 536879393 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288375, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.69, 111.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676992 reads in 214.142 CPU sec, 6.998 real sec
[M::mem_process_seqs] Processed 676992 reads in 122.065 CPU sec, 3.953 real sec
[M::process] read 676840 sequences (100000189 bp) in 2.413 CPU sec, 2.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288014, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.40, 112.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 39, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (139.44, 183.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 872)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 51, #reads = 1082658 mem = 536878437 peak = 883490816
[M::process] read 676724 sequences (100000032 bp) in 2.573 CPU sec, 2.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288555, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676624 sequences (100000045 bp) in 1.514 CPU sec, 1.516 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.19, 113.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676782 reads in 209.975 CPU sec, 6.779 real sec
[M::mem_process_seqs] Processed 676840 reads in 117.858 CPU sec, 3.775 real sec
sorting block 52, #reads = 1082306 mem = 536879492 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287647, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.82, 112.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677098 sequences (100000034 bp) in 2.992 CPU sec, 3.404 real sec...
[M::process] read 677758 sequences (100000073 bp) in 1.701 CPU sec, 1.707 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287778, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (231.95, 115.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676724 reads in 210.963 CPU sec, 6.787 real sec
[M::mem_process_seqs] Processed 676624 reads in 118.542 CPU sec, 3.785 real sec
sorting block 53, #reads = 1082337 mem = 536879971 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288116, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (225.95, 109.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677610 sequences (100000150 bp) in 2.708 CPU sec, 3.050 real sec...
[M::process] read 677448 sequences (100000122 bp) in 1.732 CPU sec, 1.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288913, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (211.76, 89.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 649)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677098 reads in 207.057 CPU sec, 6.688 real sec
[M::mem_process_seqs] Processed 677758 reads in 117.906 CPU sec, 3.783 real sec
sorting block 54, #reads = 1081525 mem = 536879703 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288076, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (216.57, 96.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1082377 mem = 536881174 peak = 883490816
[M::process] read 677292 sequences (100000114 bp) in 3.140 CPU sec, 3.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288376, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.23, 100.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677118 sequences (100000202 bp) in 1.796 CPU sec, 1.804 real sec...
[M::mem_process_seqs] Processed 677610 reads in 213.202 CPU sec, 6.840 real sec
[M::mem_process_seqs] Processed 677448 reads in 120.354 CPU sec, 3.825 real sec
sorting block 56, #reads = 1082539 mem = 536878944 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288149, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.41, 103.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677056 sequences (100000056 bp) in 2.822 CPU sec, 3.009 real sec...
[M::process] read 676818 sequences (100000062 bp) in 1.718 CPU sec, 1.726 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288214, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.52, 107.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677292 reads in 206.649 CPU sec, 6.617 real sec
[M::mem_process_seqs] Processed 677118 reads in 116.949 CPU sec, 3.733 real sec
sorting block 57, #reads = 1082676 mem = 536880921 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288289, 14, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.62, 110.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 81, 89)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199)
[M::mem_pestat] mean and std.dev: (50.64, 30.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 254)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676854 sequences (100000063 bp) in 3.153 CPU sec, 3.443 real sec...
[M::process] read 676834 sequences (100000243 bp) in 1.830 CPU sec, 1.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288263, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (228.91, 111.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677056 reads in 212.276 CPU sec, 6.808 real sec
[M::mem_process_seqs] Processed 676818 reads in 121.757 CPU sec, 3.881 real sec
sorting block 58, #reads = 1082997 mem = 536878060 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288070, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.39, 113.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676648 sequences (100000213 bp) in 2.765 CPU sec, 2.989 real sec...
[M::process] read 676490 sequences (100000159 bp) in 1.740 CPU sec, 1.750 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288148, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.52, 113.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676854 reads in 216.673 CPU sec, 6.955 real sec
[M::mem_process_seqs] Processed 676834 reads in 121.625 CPU sec, 3.873 real sec
sorting block 59, #reads = 1082698 mem = 536879927 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287679, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.02, 115.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1082576 mem = 536881297 peak = 883490816
[M::process] read 657018 sequences (97106839 bp) in 3.278 CPU sec, 3.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287941, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.23, 117.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676648 reads in 214.547 CPU sec, 6.937 real sec
[M::mem_process_seqs] Processed 676490 reads in 120.518 CPU sec, 3.900 real sec
sorting block 61, #reads = 1082384 mem = 536878708 peak = 883490816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279503, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.84, 117.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 657018 reads in 101.424 CPU sec, 3.381 real sec
sorting block 62, #reads = 1081902 mem = 536879152 peak = 883490816
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67678192
overall: 69438189568 mem 12196.065 user 797.519 sys 470.007 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L007\tPU:L007\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.T21-D0003376_R1.fastq.gz 0001.T21-D0003376_R2.fastq.gz
[main] Real time: 470.038 sec; CPU: 12993.589 sec
sorting block 63, #reads = 9778 mem = 4859198 peak = 883490816
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2645576877
output reads: 68204780
bam_mem_sort: 64 calls 51.968 user 1.425 sys 53.550 real
bam_write: 64 calls 34.211 user 2.962 sys 44.333 real
execute: 1 calls 26.082 user 1.761 sys 498.472 real
merge_files: 1 calls 26.071 user 1.721 sys 28.433 real
parse_chunk: 1009 calls 47.211 user 13.390 sys 63.098 real
read_chunk: 2716 calls 32.607 user 17.924 sys 375.000 real
sort_block: 1 calls 24.119 user 16.691 sys 362.005 real
write_chunk: 1705 calls 25.564 user 1.441 sys 27.489 real
overall: 2132119552 mem 799.189 user 61.027 sys 498.487 real
ls: cannot access '*.cram': No such file or directory