.command.err - Workdir - DAQ:SENTIEON_BWAMEM (T21-D0003376;L004;0004)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/91/62eca24b91f9f54afad94bd4dc7699/.command.err
Size: 90.7 KB
Task: DAQ:SENTIEON_BWAMEM (T21-D0003376;L004;0004)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2044.663 CPU sec, 70.972 real sec
[M::process] read 676844 sequences (100000072 bp) in 0.909 CPU sec, 0.913 real sec...
[M::process] read 676636 sequences (100000206 bp) in 1.220 CPU sec, 1.271 real sec...
[M::process] read 676508 sequences (100000165 bp) in 1.351 CPU sec, 1.359 real sec...
[M::process] read 676438 sequences (100000178 bp) in 1.207 CPU sec, 1.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288343, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.51, 113.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 91, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1662)
[M::mem_pestat] mean and std.dev: (257.30, 298.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2191)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676636 reads in 92.304 CPU sec, 2.964 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288769, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.22, 108.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676110 sequences (100000048 bp) in 1.384 CPU sec, 1.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288597, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.39, 115.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 79, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 180)
[M::mem_pestat] mean and std.dev: (58.17, 29.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 227)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676844 reads in 281.492 CPU sec, 9.032 real sec
[M::mem_process_seqs] Processed 676508 reads in 191.408 CPU sec, 6.173 real sec
sorting block 0, #reads = 1081969 mem = 536879131 peak = 617590784
[M::process] read 675880 sequences (100000028 bp) in 1.632 CPU sec, 1.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287679, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.39, 119.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676284 sequences (100000234 bp) in 1.330 CPU sec, 1.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288165, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.01, 122.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 614, 614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1660)
[M::mem_pestat] mean and std.dev: (377.09, 305.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2183)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287552, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.49, 123.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676438 reads in 262.905 CPU sec, 8.522 real sec
sorting block 1, #reads = 1081870 mem = 536880166 peak = 969125888
[M::mem_process_seqs] Processed 676110 reads in 184.816 CPU sec, 6.043 real sec
[M::mem_process_seqs] Processed 675880 reads in 192.697 CPU sec, 6.329 real sec
[M::process] read 676580 sequences (100000182 bp) in 1.552 CPU sec, 1.609 real sec...
sorting block 2, #reads = 1081902 mem = 536882195 peak = 975704064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288346, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.14, 119.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676676 sequences (100000254 bp) in 1.826 CPU sec, 2.016 real sec...
[M::process] read 676620 sequences (100000221 bp) in 1.374 CPU sec, 1.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288265, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.06, 112.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676284 reads in 182.055 CPU sec, 5.903 real sec
[M::mem_process_seqs] Processed 676580 reads in 103.202 CPU sec, 3.287 real sec
sorting block 3, #reads = 1081391 mem = 536880382 peak = 977195008
[M::process] read 676482 sequences (100000135 bp) in 1.478 CPU sec, 1.583 real sec...
sorting block 4, #reads = 1082125 mem = 536879412 peak = 984838144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288347, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.61, 111.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676354 sequences (100000266 bp) in 1.254 CPU sec, 1.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287996, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.63, 113.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288233, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.05, 113.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676676 reads in 263.774 CPU sec, 8.501 real sec
[M::mem_process_seqs] Processed 676620 reads in 185.955 CPU sec, 6.010 real sec
[M::mem_process_seqs] Processed 676482 reads in 194.106 CPU sec, 6.307 real sec
sorting block 5, #reads = 1081957 mem = 536877509 peak = 984838144
[M::process] read 676212 sequences (100000093 bp) in 1.745 CPU sec, 1.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288147, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.70, 117.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 28, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1796)
[M::mem_pestat] mean and std.dev: (253.40, 395.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2389)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676666 sequences (100000224 bp) in 1.707 CPU sec, 1.826 real sec...
[M::process] read 676532 sequences (100000283 bp) in 1.350 CPU sec, 1.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287849, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.68, 115.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676354 reads in 181.967 CPU sec, 5.917 real sec
[M::mem_process_seqs] Processed 676212 reads in 101.231 CPU sec, 3.239 real sec
sorting block 6, #reads = 1082298 mem = 536878256 peak = 984838144
sorting block 7, #reads = 1081984 mem = 536881251 peak = 998842368
[M::process] read 676214 sequences (100000021 bp) in 1.690 CPU sec, 1.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287851, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.80, 111.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 26, 93)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257)
[M::mem_pestat] mean and std.dev: (31.67, 34.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 339)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676176 sequences (100000025 bp) in 1.370 CPU sec, 1.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287777, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.76, 116.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287869, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.93, 114.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (103, 229, 619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1651)
[M::mem_pestat] mean and std.dev: (332.38, 314.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2167)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676666 reads in 267.161 CPU sec, 8.654 real sec
[M::mem_process_seqs] Processed 676214 reads in 188.153 CPU sec, 6.111 real sec
[M::mem_process_seqs] Processed 676532 reads in 196.246 CPU sec, 6.393 real sec
sorting block 8, #reads = 1081886 mem = 536880917 peak = 998842368
[M::process] read 675874 sequences (100000064 bp) in 2.072 CPU sec, 2.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287401, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (240.41, 121.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1081804 mem = 536878977 peak = 1000341504
[M::process] read 676026 sequences (100000100 bp) in 1.694 CPU sec, 1.794 real sec...
[M::process] read 676128 sequences (100000088 bp) in 1.377 CPU sec, 1.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287313, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (245.32, 124.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 41, 134)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (44.09, 44.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676176 reads in 190.425 CPU sec, 6.200 real sec
[M::mem_process_seqs] Processed 675874 reads in 107.580 CPU sec, 3.461 real sec
sorting block 10, #reads = 1081674 mem = 536878355 peak = 1000341504
[M::process] read 676876 sequences (100000014 bp) in 1.896 CPU sec, 2.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287827, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.45, 123.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676730 sequences (100000253 bp) in 1.418 CPU sec, 1.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287172, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.36, 121.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287914, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.74, 107.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676026 reads in 273.371 CPU sec, 8.807 real sec
[M::mem_process_seqs] Processed 676128 reads in 191.338 CPU sec, 6.161 real sec
[M::mem_process_seqs] Processed 676876 reads in 198.809 CPU sec, 6.423 real sec
sorting block 11, #reads = 1082120 mem = 536879399 peak = 1000341504
[M::process] read 676462 sequences (100000222 bp) in 1.768 CPU sec, 1.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288230, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.27, 112.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 12, #reads = 1082383 mem = 536878782 peak = 1022844928
[M::process] read 676412 sequences (100000273 bp) in 2.013 CPU sec, 2.206 real sec...
[M::process] read 676352 sequences (100000210 bp) in 1.431 CPU sec, 1.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288275, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.73, 115.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676730 reads in 186.449 CPU sec, 6.001 real sec
[M::mem_process_seqs] Processed 676462 reads in 104.000 CPU sec, 3.328 real sec
sorting block 13, #reads = 1082185 mem = 536879272 peak = 1022844928
[M::process] read 676574 sequences (100000097 bp) in 1.819 CPU sec, 1.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287852, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.56, 117.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676628 sequences (100000036 bp) in 1.397 CPU sec, 1.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287325, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.99, 119.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288098, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.96, 115.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676412 reads in 264.586 CPU sec, 8.528 real sec
[M::mem_process_seqs] Processed 676352 reads in 186.223 CPU sec, 6.001 real sec
[M::mem_process_seqs] Processed 676574 reads in 194.151 CPU sec, 6.288 real sec
sorting block 14, #reads = 1082269 mem = 536880658 peak = 1022844928
[M::process] read 676844 sequences (100000277 bp) in 1.778 CPU sec, 1.946 real sec...
sorting block 15, #reads = 1081556 mem = 536877465 peak = 1023627264
[M::process] read 676574 sequences (100000058 bp) in 1.933 CPU sec, 2.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288529, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.01, 110.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676384 sequences (100000198 bp) in 1.502 CPU sec, 1.510 real sec...
[M::mem_process_seqs] Processed 676844 reads in 97.534 CPU sec, 3.102 real sec
sorting block 16, #reads = 1082701 mem = 536880118 peak = 1023627264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288330, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (232.27, 111.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 101, 2158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6444)
[M::mem_pestat] mean and std.dev: (897.36, 1045.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8587)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676096 sequences (100000044 bp) in 1.457 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287874, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.34, 112.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676628 reads in 279.964 CPU sec, 9.130 real sec
[M::mem_process_seqs] Processed 676574 reads in 112.549 CPU sec, 3.770 real sec
sorting block 17, #reads = 1081896 mem = 536879002 peak = 1023627264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288205, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.93, 117.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676202 sequences (100000251 bp) in 2.029 CPU sec, 2.260 real sec...
[M::process] read 676626 sequences (100000003 bp) in 1.412 CPU sec, 1.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287871, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.91, 120.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676384 reads in 186.157 CPU sec, 6.016 real sec
[M::mem_process_seqs] Processed 676096 reads in 105.065 CPU sec, 3.389 real sec
sorting block 18, #reads = 1082889 mem = 536878667 peak = 1023627264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287441, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (240.71, 120.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676832 sequences (100000031 bp) in 2.166 CPU sec, 2.397 real sec...
[M::process] read 676604 sequences (100000052 bp) in 1.455 CPU sec, 1.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288137, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.12, 115.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676202 reads in 187.557 CPU sec, 6.025 real sec
[M::mem_process_seqs] Processed 676626 reads in 104.081 CPU sec, 3.335 real sec
sorting block 19, #reads = 1081914 mem = 536878574 peak = 1023627264
[M::process] read 676478 sequences (100000159 bp) in 1.784 CPU sec, 1.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288203, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.81, 109.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676296 sequences (100000126 bp) in 1.633 CPU sec, 1.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287838, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.79, 114.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 76, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1798)
[M::mem_pestat] mean and std.dev: (246.35, 362.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2392)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676832 reads in 188.001 CPU sec, 6.041 real sec
[M::mem_process_seqs] Processed 676604 reads in 105.997 CPU sec, 3.389 real sec
sorting block 20, #reads = 1082439 mem = 536881837 peak = 1023627264
sorting block 21, #reads = 1082253 mem = 536877361 peak = 1048195072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287490, 13, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.43, 116.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 70, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1503)
[M::mem_pestat] mean and std.dev: (203.92, 242.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1998)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676206 sequences (100000214 bp) in 2.149 CPU sec, 2.336 real sec...
[M::process] read 676986 sequences (100000065 bp) in 1.407 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287756, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.58, 118.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676478 reads in 188.937 CPU sec, 6.099 real sec
[M::mem_process_seqs] Processed 676296 reads in 106.565 CPU sec, 3.442 real sec
sorting block 22, #reads = 1082313 mem = 536879258 peak = 1048195072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287812, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.41, 118.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677156 sequences (100000214 bp) in 1.998 CPU sec, 2.154 real sec...
[M::process] read 676888 sequences (100000274 bp) in 1.475 CPU sec, 1.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288277, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.69, 108.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676206 reads in 185.325 CPU sec, 5.970 real sec
[M::mem_process_seqs] Processed 676986 reads in 103.940 CPU sec, 3.369 real sec
sorting block 23, #reads = 1081480 mem = 536879146 peak = 1048195072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288229, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (226.10, 105.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676636 sequences (100000205 bp) in 2.164 CPU sec, 2.351 real sec...
[M::process] read 676362 sequences (100000048 bp) in 1.481 CPU sec, 1.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288511, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.38, 105.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677156 reads in 186.639 CPU sec, 6.014 real sec
[M::mem_process_seqs] Processed 676888 reads in 104.884 CPU sec, 3.386 real sec
sorting block 24, #reads = 1082621 mem = 536876587 peak = 1048195072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288023, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.44, 110.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676302 sequences (100000206 bp) in 1.830 CPU sec, 2.242 real sec...
[M::process] read 676522 sequences (100000188 bp) in 1.469 CPU sec, 1.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288224, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.99, 114.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676636 reads in 186.247 CPU sec, 6.045 real sec
[M::mem_process_seqs] Processed 676362 reads in 105.271 CPU sec, 3.385 real sec
sorting block 25, #reads = 1082781 mem = 536879098 peak = 1048195072
sorting block 26, #reads = 1082322 mem = 536877685 peak = 1048195072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287686, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (235.58, 115.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 89, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (165.70, 136.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1333)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676774 sequences (100000149 bp) in 2.253 CPU sec, 2.439 real sec...
[M::process] read 676878 sequences (100000105 bp) in 1.491 CPU sec, 1.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287939, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (235.24, 115.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676302 reads in 186.375 CPU sec, 5.983 real sec
[M::mem_process_seqs] Processed 676522 reads in 104.396 CPU sec, 3.335 real sec
sorting block 27, #reads = 1081674 mem = 536878586 peak = 1048195072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288079, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.84, 108.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676522 sequences (100000033 bp) in 2.036 CPU sec, 2.376 real sec...
[M::process] read 676340 sequences (100000016 bp) in 1.474 CPU sec, 1.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288574, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.30, 108.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676774 reads in 188.266 CPU sec, 6.101 real sec
[M::mem_process_seqs] Processed 676878 reads in 106.249 CPU sec, 3.449 real sec
sorting block 28, #reads = 1082772 mem = 536878656 peak = 1048195072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288288, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (232.03, 112.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676168 sequences (100000262 bp) in 2.204 CPU sec, 2.380 real sec...
[M::process] read 676162 sequences (100000143 bp) in 1.504 CPU sec, 1.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287783, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.77, 116.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 89, 1660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4900)
[M::mem_pestat] mean and std.dev: (597.59, 714.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6520)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676522 reads in 187.673 CPU sec, 6.031 real sec
[M::mem_process_seqs] Processed 676340 reads in 105.303 CPU sec, 3.369 real sec
sorting block 29, #reads = 1082878 mem = 536880278 peak = 1048195072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287229, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (239.05, 119.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 190, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580)
[M::mem_pestat] mean and std.dev: (108.44, 111.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676922 sequences (100000238 bp) in 1.985 CPU sec, 2.255 real sec...
[M::process] read 676796 sequences (100000093 bp) in 1.481 CPU sec, 1.486 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287725, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.10, 118.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676168 reads in 186.025 CPU sec, 5.983 real sec
[M::mem_process_seqs] Processed 676162 reads in 104.117 CPU sec, 3.324 real sec
sorting block 30, #reads = 1082000 mem = 536879953 peak = 1072185344
sorting block 31, #reads = 1081856 mem = 536876835 peak = 1074872320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287980, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.49, 112.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 187, 945)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2795)
[M::mem_pestat] mean and std.dev: (363.91, 383.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3720)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676620 sequences (100000192 bp) in 2.211 CPU sec, 2.446 real sec...
[M::process] read 676506 sequences (100000003 bp) in 1.459 CPU sec, 1.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287646, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.11, 115.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676922 reads in 187.700 CPU sec, 6.056 real sec
[M::mem_process_seqs] Processed 676796 reads in 107.310 CPU sec, 3.443 real sec
sorting block 32, #reads = 1082191 mem = 536876958 peak = 1074872320
[M::process] read 676328 sequences (100000118 bp) in 2.057 CPU sec, 2.282 real sec...
[M::process] read 676170 sequences (100000007 bp) in 1.480 CPU sec, 1.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286812, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.91, 117.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 161, 161)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (90.00, 103.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676506 reads in 101.303 CPU sec, 3.238 real sec
sorting block 33, #reads = 1081439 mem = 536876717 peak = 1074872320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287923, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.07, 116.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676386 sequences (100000118 bp) in 1.422 CPU sec, 1.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287218, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (242.94, 123.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676620 reads in 289.854 CPU sec, 9.351 real sec
[M::mem_process_seqs] Processed 676328 reads in 114.956 CPU sec, 3.738 real sec
sorting block 34, #reads = 1081956 mem = 536877928 peak = 1074872320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287447, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.33, 122.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676846 sequences (100000009 bp) in 2.445 CPU sec, 2.902 real sec...
[M::process] read 676796 sequences (100000200 bp) in 1.497 CPU sec, 1.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287456, 15, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.13, 120.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 28, 125)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319)
[M::mem_pestat] mean and std.dev: (38.42, 39.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 416)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676170 reads in 190.249 CPU sec, 6.134 real sec
[M::mem_process_seqs] Processed 676386 reads in 107.335 CPU sec, 3.426 real sec
sorting block 35, #reads = 1081615 mem = 536881779 peak = 1074872320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288096, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.45, 110.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 35, 188)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (110.54, 146.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676842 sequences (100000298 bp) in 1.962 CPU sec, 2.201 real sec...
[M::process] read 676700 sequences (100000176 bp) in 1.405 CPU sec, 1.486 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287877, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.81, 110.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676846 reads in 189.398 CPU sec, 6.087 real sec
[M::mem_process_seqs] Processed 676796 reads in 105.731 CPU sec, 3.397 real sec
sorting block 36, #reads = 1082829 mem = 536879741 peak = 1074872320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287666, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.33, 111.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676656 sequences (100000269 bp) in 2.166 CPU sec, 2.553 real sec...
[M::process] read 676818 sequences (100000135 bp) in 1.511 CPU sec, 1.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287666, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.51, 113.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676842 reads in 189.096 CPU sec, 6.081 real sec
[M::mem_process_seqs] Processed 676700 reads in 105.493 CPU sec, 3.361 real sec
sorting block 37, #reads = 1082062 mem = 536878970 peak = 1074872320
sorting block 38, #reads = 1082160 mem = 536877987 peak = 1086054400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288110, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.97, 114.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676804 sequences (100000272 bp) in 1.844 CPU sec, 2.160 real sec...
[M::process] read 676664 sequences (100000267 bp) in 1.394 CPU sec, 1.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287838, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.90, 113.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 58, 169)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (95.30, 97.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 502)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676656 reads in 187.107 CPU sec, 6.045 real sec
[M::mem_process_seqs] Processed 676818 reads in 106.404 CPU sec, 3.438 real sec
sorting block 39, #reads = 1082184 mem = 536880287 peak = 1086054400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287449, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.81, 115.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 107, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (152.20, 150.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1380)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676766 sequences (100000031 bp) in 2.257 CPU sec, 2.593 real sec...
[M::process] read 676450 sequences (100000127 bp) in 1.533 CPU sec, 1.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287849, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.30, 114.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676804 reads in 188.010 CPU sec, 6.093 real sec
[M::mem_process_seqs] Processed 676664 reads in 105.518 CPU sec, 3.409 real sec
sorting block 40, #reads = 1081583 mem = 536880130 peak = 1086054400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287924, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.08, 115.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 32, 626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868)
[M::mem_pestat] mean and std.dev: (236.80, 341.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2489)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676146 sequences (100000035 bp) in 1.844 CPU sec, 2.149 real sec...
[M::process] read 676434 sequences (100000029 bp) in 1.495 CPU sec, 1.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287310, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.42, 120.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 493, 783)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2247)
[M::mem_pestat] mean and std.dev: (359.25, 279.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2979)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676766 reads in 191.688 CPU sec, 6.185 real sec
[M::mem_process_seqs] Processed 676450 reads in 108.826 CPU sec, 3.474 real sec
sorting block 41, #reads = 1082201 mem = 536877857 peak = 1086054400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287792, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.08, 124.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676746 sequences (100000235 bp) in 2.120 CPU sec, 2.322 real sec...
[M::process] read 677186 sequences (100000218 bp) in 1.527 CPU sec, 1.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287118, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.52, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676146 reads in 194.768 CPU sec, 6.262 real sec
[M::mem_process_seqs] Processed 676434 reads in 109.374 CPU sec, 3.511 real sec
sorting block 42, #reads = 1082119 mem = 536877272 peak = 1098051584
sorting block 43, #reads = 1082184 mem = 536878159 peak = 1098051584
[M::process] read 676954 sequences (100000171 bp) in 1.852 CPU sec, 2.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287605, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.20, 111.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676838 sequences (100000263 bp) in 1.450 CPU sec, 1.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288332, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.27, 103.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676746 reads in 191.921 CPU sec, 6.203 real sec
[M::mem_process_seqs] Processed 677186 reads in 106.968 CPU sec, 3.440 real sec
sorting block 44, #reads = 1082732 mem = 536878152 peak = 1098051584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287605, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.23, 109.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 51, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163)
[M::mem_pestat] mean and std.dev: (34.50, 21.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 214)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676710 sequences (100000299 bp) in 2.293 CPU sec, 2.531 real sec...
[M::process] read 676822 sequences (100000145 bp) in 1.579 CPU sec, 1.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287960, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.85, 112.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676954 reads in 192.535 CPU sec, 6.188 real sec
[M::mem_process_seqs] Processed 676838 reads in 106.797 CPU sec, 3.423 real sec
sorting block 45, #reads = 1082707 mem = 536879567 peak = 1098051584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287444, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.29, 113.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 45, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144)
[M::mem_pestat] mean and std.dev: (193.30, 210.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1520)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676848 sequences (100000139 bp) in 2.001 CPU sec, 2.299 real sec...
[M::process] read 676968 sequences (100000221 bp) in 1.552 CPU sec, 1.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287419, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (227.86, 111.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676710 reads in 190.301 CPU sec, 6.142 real sec
[M::mem_process_seqs] Processed 676822 reads in 106.198 CPU sec, 3.399 real sec
sorting block 46, #reads = 1082524 mem = 536878154 peak = 1098051584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288113, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.32, 110.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677044 sequences (100000006 bp) in 2.206 CPU sec, 2.598 real sec...
[M::process] read 676880 sequences (100000250 bp) in 1.419 CPU sec, 1.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287785, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.14, 110.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676848 reads in 191.037 CPU sec, 6.162 real sec
[M::mem_process_seqs] Processed 676968 reads in 108.329 CPU sec, 3.469 real sec
sorting block 47, #reads = 1081243 mem = 536878772 peak = 1098051584
sorting block 48, #reads = 1082396 mem = 536878189 peak = 1106075648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287582, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.43, 108.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676536 sequences (100000091 bp) in 1.965 CPU sec, 2.375 real sec...
[M::process] read 676362 sequences (100000191 bp) in 1.462 CPU sec, 1.502 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287128, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.57, 110.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677044 reads in 191.340 CPU sec, 6.194 real sec
[M::mem_process_seqs] Processed 676880 reads in 106.211 CPU sec, 3.405 real sec
sorting block 49, #reads = 1082467 mem = 536876934 peak = 1106075648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287739, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.21, 117.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676634 sequences (100000294 bp) in 2.247 CPU sec, 2.674 real sec...
[M::process] read 677070 sequences (100000231 bp) in 1.570 CPU sec, 1.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286917, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.22, 120.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676536 reads in 198.414 CPU sec, 6.401 real sec
[M::mem_process_seqs] Processed 676362 reads in 111.983 CPU sec, 3.589 real sec
sorting block 50, #reads = 1081716 mem = 536877827 peak = 1106075648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287475, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.97, 117.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676860 sequences (100000184 bp) in 1.982 CPU sec, 2.344 real sec...
[M::process] read 676858 sequences (100000282 bp) in 1.542 CPU sec, 1.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287954, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.72, 102.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676634 reads in 191.760 CPU sec, 6.167 real sec
[M::mem_process_seqs] Processed 677070 reads in 106.062 CPU sec, 3.387 real sec
sorting block 51, #reads = 1082312 mem = 536879992 peak = 1106075648
[M::process] read 676754 sequences (100000242 bp) in 2.268 CPU sec, 2.641 real sec...
[M::process] read 676698 sequences (100000110 bp) in 1.589 CPU sec, 1.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287764, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.57, 111.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 78, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462)
[M::mem_pestat] mean and std.dev: (89.70, 54.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 590)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676858 reads in 105.027 CPU sec, 3.356 real sec
sorting block 52, #reads = 1082475 mem = 536876564 peak = 1116721152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287908, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.47, 110.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677194 sequences (100000286 bp) in 1.507 CPU sec, 1.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287941, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.09, 113.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676860 reads in 291.861 CPU sec, 9.443 real sec
[M::mem_process_seqs] Processed 676754 reads in 117.088 CPU sec, 3.826 real sec
sorting block 53, #reads = 1082487 mem = 536877942 peak = 1116721152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287774, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (231.93, 115.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677306 sequences (100000151 bp) in 2.216 CPU sec, 2.454 real sec...
[M::process] read 677194 sequences (100000118 bp) in 1.591 CPU sec, 1.670 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287770, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.62, 108.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676698 reads in 184.638 CPU sec, 5.932 real sec
[M::mem_process_seqs] Processed 677194 reads in 104.006 CPU sec, 3.319 real sec
sorting block 54, #reads = 1082157 mem = 536879797 peak = 1116721152
sorting block 55, #reads = 1080767 mem = 536879354 peak = 1127780352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288409, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.12, 106.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 41, 176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476)
[M::mem_pestat] mean and std.dev: (43.00, 51.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 626)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677006 sequences (100000075 bp) in 1.969 CPU sec, 2.188 real sec...
[M::process] read 676840 sequences (100000174 bp) in 1.515 CPU sec, 1.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288184, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.34, 106.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677306 reads in 183.931 CPU sec, 5.930 real sec
[M::mem_process_seqs] Processed 677194 reads in 102.928 CPU sec, 3.302 real sec
sorting block 56, #reads = 1082312 mem = 536880572 peak = 1127780352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287804, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.87, 107.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 102, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1866)
[M::mem_pestat] mean and std.dev: (281.00, 303.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2471)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676676 sequences (100000159 bp) in 2.123 CPU sec, 2.389 real sec...
[M::process] read 676676 sequences (100000249 bp) in 1.576 CPU sec, 1.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287773, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.64, 112.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677006 reads in 191.507 CPU sec, 6.165 real sec
[M::mem_process_seqs] Processed 676840 reads in 107.383 CPU sec, 3.442 real sec
sorting block 57, #reads = 1081984 mem = 536881008 peak = 1127780352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287305, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.34, 114.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677000 sequences (100000113 bp) in 2.118 CPU sec, 2.315 real sec...
[M::process] read 676962 sequences (100000004 bp) in 1.580 CPU sec, 1.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287157, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.91, 114.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676676 reads in 196.685 CPU sec, 6.334 real sec
[M::mem_process_seqs] Processed 676676 reads in 109.920 CPU sec, 3.525 real sec
sorting block 58, #reads = 1081982 mem = 536878434 peak = 1127780352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288054, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.73, 107.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676812 sequences (100000043 bp) in 2.362 CPU sec, 2.634 real sec...
[M::process] read 676556 sequences (100000152 bp) in 1.567 CPU sec, 1.598 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288261, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.08, 108.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677000 reads in 188.470 CPU sec, 6.058 real sec
[M::mem_process_seqs] Processed 676962 reads in 105.162 CPU sec, 3.352 real sec
sorting block 59, #reads = 1081718 mem = 536879191 peak = 1127780352
sorting block 60, #reads = 1082356 mem = 536877796 peak = 1127780352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287759, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.09, 112.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676472 sequences (100000219 bp) in 1.977 CPU sec, 2.167 real sec...
[M::process] read 552014 sequences (81593087 bp) in 1.343 CPU sec, 1.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287505, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.02, 115.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 81, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124)
[M::mem_pestat] mean and std.dev: (145.33, 224.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1498)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676812 reads in 192.283 CPU sec, 6.201 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287834, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.53, 117.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676472 reads in 98.273 CPU sec, 3.194 real sec
sorting block 61, #reads = 1082896 mem = 536878662 peak = 1127780352
[M::mem_process_seqs] Processed 676556 reads in 241.280 CPU sec, 7.811 real sec
sorting block 62, #reads = 1082220 mem = 536880802 peak = 1127780352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 234785, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.55, 116.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 552014 reads in 85.139 CPU sec, 2.794 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 68213238
overall: 69781729280 mem 11481.546 user 427.366 sys 398.154 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L004\tPU:L004\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.T21-D0003376_R1.fastq.gz 0004.T21-D0003376_R2.fastq.gz
[main] Real time: 398.187 sec; CPU: 11908.914 sec
sorting block 63, #reads = 562609 mem = 279244363 peak = 1127780352
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2648618046
output reads: 68736745
bam_mem_sort: 64 calls 43.588 user 1.255 sys 46.018 real
bam_write: 64 calls 35.229 user 2.930 sys 71.656 real
execute: 1 calls 19.691 user 0.938 sys 419.858 real
merge_files: 1 calls 19.650 user 0.934 sys 21.226 real
parse_chunk: 1017 calls 47.169 user 11.366 sys 70.858 real
read_chunk: 2738 calls 21.989 user 12.644 sys 328.669 real
sort_block: 1 calls 15.412 user 11.998 sys 320.611 real
write_chunk: 1719 calls 19.273 user 0.751 sys 20.467 real
overall: 2522951680 mem 706.623 user 43.311 sys 419.877 real
ls: cannot access '*.cram': No such file or directory