Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/c2f7d9701f4e3120d706aa711c6696/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/55/5fb6125a0fac831f87822a1d36f460/T21-D0003376/0002.T21-D0003376_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/c2f7d9701f4e3120d706aa711c6696/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/55/5fb6125a0fac831f87822a1d36f460/T21-D0003376/0002.T21-D0003376_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
Received only 8 licenses out of 31 requested for sentieon:klib
[M::main_mem] bwa index loaded in 2469.588 CPU sec, 556.592 real sec
[M::process] read 676946 sequences (100000114 bp) in 1.324 CPU sec, 1.332 real sec...
[M::process] read 676780 sequences (100000055 bp) in 1.510 CPU sec, 1.509 real sec...
[M::process] read 676738 sequences (100000203 bp) in 1.377 CPU sec, 1.378 real sec...
[M::process] read 677062 sequences (100000202 bp) in 1.340 CPU sec, 1.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287927, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.24, 108.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288230, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.40, 112.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288087, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.93, 112.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676946 reads in 372.926 CPU sec, 11.974 real sec
[M::mem_process_seqs] Processed 676738 reads in 222.928 CPU sec, 7.246 real sec
[M::mem_process_seqs] Processed 676780 reads in 232.366 CPU sec, 7.583 real sec
sorting block 0, #reads = 1082115 mem = 536878594 peak = 635629568
[M::process] read 676956 sequences (100000134 bp) in 2.734 CPU sec, 3.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287852, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.35, 108.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 13, 38)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 94)
[M::mem_pestat] mean and std.dev: (15.33, 9.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 122)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677008 sequences (100000281 bp) in 1.956 CPU sec, 2.147 real sec...
[M::process] read 676866 sequences (100000206 bp) in 1.323 CPU sec, 1.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288282, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.19, 109.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 80, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (89.75, 94.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677062 reads in 221.286 CPU sec, 7.196 real sec
sorting block 1, #reads = 1082239 mem = 536878826 peak = 783478784
[M::mem_process_seqs] Processed 676956 reads in 127.156 CPU sec, 4.181 real sec
sorting block 2, #reads = 1082493 mem = 536878883 peak = 866631680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288395, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.85, 109.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 340, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317)
[M::mem_pestat] mean and std.dev: (198.00, 220.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1755)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676882 sequences (100000264 bp) in 3.003 CPU sec, 3.274 real sec...
[M::process] read 676792 sequences (100000066 bp) in 1.770 CPU sec, 1.772 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288095, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.64, 109.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 71, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (109.08, 115.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1336)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677008 reads in 221.773 CPU sec, 7.080 real sec
[M::mem_process_seqs] Processed 676866 reads in 122.805 CPU sec, 3.924 real sec
sorting block 3, #reads = 1082732 mem = 536880741 peak = 866631680
[M::process] read 676048 sequences (100000201 bp) in 2.248 CPU sec, 2.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288346, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.43, 110.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676290 sequences (100000003 bp) in 1.130 CPU sec, 1.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288398, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.68, 111.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287517, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.19, 124.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676882 reads in 326.916 CPU sec, 10.548 real sec
[M::mem_process_seqs] Processed 676792 reads in 227.208 CPU sec, 7.375 real sec
[M::mem_process_seqs] Processed 676048 reads in 235.275 CPU sec, 7.668 real sec
sorting block 4, #reads = 1082182 mem = 536879158 peak = 866631680
[M::process] read 676114 sequences (100000193 bp) in 2.882 CPU sec, 3.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287601, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.61, 119.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 5, #reads = 1082289 mem = 536877411 peak = 866631680
[M::process] read 676562 sequences (100000180 bp) in 2.526 CPU sec, 2.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287118, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (241.90, 122.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676290 reads in 243.410 CPU sec, 7.796 real sec
[M::process] read 676836 sequences (100000176 bp) in 1.739 CPU sec, 1.757 real sec...
[M::mem_process_seqs] Processed 676114 reads in 137.731 CPU sec, 4.385 real sec
sorting block 6, #reads = 1082066 mem = 536878781 peak = 866631680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287727, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.62, 114.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676686 sequences (100000230 bp) in 3.536 CPU sec, 4.009 real sec...
[M::process] read 676732 sequences (100000060 bp) in 1.740 CPU sec, 1.743 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288271, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.02, 112.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 479, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1387)
[M::mem_pestat] mean and std.dev: (360.31, 369.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1841)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676562 reads in 238.858 CPU sec, 7.650 real sec
[M::mem_process_seqs] Processed 676836 reads in 134.957 CPU sec, 4.326 real sec
sorting block 7, #reads = 1081075 mem = 536880183 peak = 866631680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288078, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.92, 113.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 684, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1902)
[M::mem_pestat] mean and std.dev: (412.56, 303.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2511)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677036 sequences (100000092 bp) in 2.775 CPU sec, 3.102 real sec...
[M::process] read 677018 sequences (100000159 bp) in 1.960 CPU sec, 1.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288448, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.82, 111.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676686 reads in 233.680 CPU sec, 7.493 real sec
[M::mem_process_seqs] Processed 676732 reads in 128.840 CPU sec, 4.127 real sec
sorting block 8, #reads = 1082210 mem = 536878890 peak = 866631680
sorting block 9, #reads = 1081968 mem = 536880367 peak = 876363776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287905, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.21, 109.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676976 sequences (100000177 bp) in 2.901 CPU sec, 3.086 real sec...
[M::process] read 677004 sequences (100000051 bp) in 1.450 CPU sec, 1.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288248, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.59, 110.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677036 reads in 232.801 CPU sec, 7.471 real sec
[M::mem_process_seqs] Processed 677018 reads in 128.545 CPU sec, 4.134 real sec
sorting block 10, #reads = 1082816 mem = 536879725 peak = 876363776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288301, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.30, 110.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676782 sequences (100000297 bp) in 2.753 CPU sec, 2.986 real sec...
[M::process] read 676260 sequences (100000290 bp) in 1.540 CPU sec, 1.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287648, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.26, 110.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676976 reads in 227.181 CPU sec, 7.277 real sec
[M::mem_process_seqs] Processed 677004 reads in 121.552 CPU sec, 3.892 real sec
sorting block 11, #reads = 1082321 mem = 536880217 peak = 876363776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288151, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.51, 112.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676376 sequences (100000117 bp) in 2.810 CPU sec, 3.057 real sec...
[M::process] read 676016 sequences (100000126 bp) in 1.664 CPU sec, 1.669 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287317, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.32, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 218, 628)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1670)
[M::mem_pestat] mean and std.dev: (278.00, 223.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2191)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676782 reads in 226.055 CPU sec, 7.248 real sec
[M::mem_process_seqs] Processed 676260 reads in 123.269 CPU sec, 3.943 real sec
sorting block 12, #reads = 1082293 mem = 536880053 peak = 876363776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287751, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.71, 119.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676360 sequences (100000074 bp) in 3.016 CPU sec, 3.450 real sec...
[M::process] read 677018 sequences (100000295 bp) in 1.440 CPU sec, 1.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287405, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (242.87, 123.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676376 reads in 230.064 CPU sec, 7.399 real sec
[M::mem_process_seqs] Processed 676016 reads in 130.664 CPU sec, 4.224 real sec
sorting block 13, #reads = 1082316 mem = 536878854 peak = 876363776
sorting block 14, #reads = 1082136 mem = 536877763 peak = 904847360
[M::process] read 676628 sequences (100000246 bp) in 2.619 CPU sec, 2.832 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288058, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.59, 120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676588 sequences (100000176 bp) in 1.880 CPU sec, 1.886 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288629, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.96, 110.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 20, 75)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 221)
[M::mem_pestat] mean and std.dev: (29.78, 35.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 294)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288107, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.78, 115.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676360 reads in 333.381 CPU sec, 10.713 real sec
[M::mem_process_seqs] Processed 677018 reads in 228.791 CPU sec, 7.375 real sec
[M::mem_process_seqs] Processed 676628 reads in 236.966 CPU sec, 7.660 real sec
sorting block 15, #reads = 1081902 mem = 536878426 peak = 904847360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288340, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.19, 115.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676788 sequences (100000277 bp) in 3.222 CPU sec, 3.642 real sec...
[M::mem_process_seqs] Processed 676588 reads in 109.948 CPU sec, 3.645 real sec
sorting block 16, #reads = 1082284 mem = 536878326 peak = 904847360
[M::process] read 677034 sequences (100000252 bp) in 2.739 CPU sec, 3.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287841, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.64, 112.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676852 sequences (100000125 bp) in 1.578 CPU sec, 1.627 real sec...
[M::process] read 676854 sequences (100000292 bp) in 1.460 CPU sec, 1.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288306, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.36, 110.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676788 reads in 215.909 CPU sec, 6.910 real sec
[M::mem_process_seqs] Processed 677034 reads in 119.104 CPU sec, 3.794 real sec
sorting block 17, #reads = 1081969 mem = 536879640 peak = 904847360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288404, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.71, 111.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676786 sequences (100000215 bp) in 3.674 CPU sec, 3.977 real sec...
[M::process] read 676628 sequences (100000140 bp) in 1.829 CPU sec, 1.833 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288432, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.65, 112.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676852 reads in 228.220 CPU sec, 7.302 real sec
sorting block 18, #reads = 1082447 mem = 536878789 peak = 904847360
[M::mem_process_seqs] Processed 676854 reads in 125.784 CPU sec, 4.008 real sec
sorting block 19, #reads = 1082800 mem = 536878324 peak = 920121344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288087, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.07, 112.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676556 sequences (100000055 bp) in 2.419 CPU sec, 2.701 real sec...
[M::process] read 676372 sequences (100000204 bp) in 1.640 CPU sec, 1.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287978, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.93, 115.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676786 reads in 222.444 CPU sec, 7.133 real sec
[M::mem_process_seqs] Processed 676628 reads in 121.830 CPU sec, 3.909 real sec
sorting block 20, #reads = 1082703 mem = 536879201 peak = 920121344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287959, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.97, 117.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676296 sequences (100000147 bp) in 3.028 CPU sec, 3.308 real sec...
[M::process] read 676482 sequences (100000007 bp) in 2.250 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288139, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.87, 117.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676556 reads in 226.867 CPU sec, 7.256 real sec
[M::mem_process_seqs] Processed 676372 reads in 125.374 CPU sec, 4.030 real sec
sorting block 21, #reads = 1081902 mem = 536881531 peak = 920121344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288019, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.84, 118.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676744 sequences (100000077 bp) in 2.528 CPU sec, 2.772 real sec...
[M::process] read 676522 sequences (100000264 bp) in 1.680 CPU sec, 1.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288435, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.54, 114.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 91, 182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532)
[M::mem_pestat] mean and std.dev: (93.00, 78.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 707)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676296 reads in 201.584 CPU sec, 6.458 real sec
[M::mem_process_seqs] Processed 676482 reads in 110.232 CPU sec, 3.513 real sec
sorting block 22, #reads = 1082207 mem = 536878349 peak = 920121344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287935, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.75, 112.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 35, 832)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2440)
[M::mem_pestat] mean and std.dev: (326.55, 540.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3244)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676318 sequences (100000071 bp) in 3.233 CPU sec, 3.466 real sec...
[M::process] read 676876 sequences (100000207 bp) in 2.060 CPU sec, 2.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288276, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.07, 114.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676744 reads in 207.216 CPU sec, 6.618 real sec
sorting block 23, #reads = 1082178 mem = 536885855 peak = 920121344
[M::mem_process_seqs] Processed 676522 reads in 115.519 CPU sec, 3.681 real sec
sorting block 24, #reads = 1082094 mem = 536879564 peak = 959561728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287847, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.18, 116.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 179, 179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (89.00, 74.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 596)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676946 sequences (100000154 bp) in 2.525 CPU sec, 2.739 real sec...
[M::process] read 676754 sequences (100000170 bp) in 1.750 CPU sec, 1.750 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288332, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (229.04, 109.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 58, 58)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (27.75, 23.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 202)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676318 reads in 209.910 CPU sec, 6.715 real sec
[M::mem_process_seqs] Processed 676876 reads in 117.419 CPU sec, 3.746 real sec
sorting block 25, #reads = 1082657 mem = 536879520 peak = 959561728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288237, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.53, 110.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676640 sequences (100000285 bp) in 2.965 CPU sec, 3.216 real sec...
[M::process] read 676642 sequences (100000132 bp) in 1.940 CPU sec, 1.946 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288307, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.98, 111.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676946 reads in 210.383 CPU sec, 6.721 real sec
[M::mem_process_seqs] Processed 676754 reads in 115.745 CPU sec, 3.680 real sec
sorting block 26, #reads = 1082148 mem = 536879581 peak = 959561728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288210, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.64, 112.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676508 sequences (100000000 bp) in 2.535 CPU sec, 2.767 real sec...
[M::process] read 676372 sequences (100000044 bp) in 2.010 CPU sec, 2.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287939, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.41, 113.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676640 reads in 210.995 CPU sec, 6.752 real sec
[M::mem_process_seqs] Processed 676642 reads in 115.609 CPU sec, 3.684 real sec
sorting block 27, #reads = 1082573 mem = 536879448 peak = 959561728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288385, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.21, 114.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676282 sequences (100000099 bp) in 3.225 CPU sec, 3.473 real sec...
[M::process] read 676042 sequences (100000256 bp) in 1.659 CPU sec, 1.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288329, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.67, 117.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (343, 998, 2658)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7288)
[M::mem_pestat] mean and std.dev: (1549.40, 1439.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9603)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676508 reads in 202.577 CPU sec, 6.472 real sec
sorting block 28, #reads = 1081900 mem = 536878051 peak = 959561728
[M::mem_process_seqs] Processed 676372 reads in 114.186 CPU sec, 3.639 real sec
sorting block 29, #reads = 1082621 mem = 536879059 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288187, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.29, 117.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677054 sequences (100000274 bp) in 2.975 CPU sec, 3.235 real sec...
[M::process] read 676828 sequences (100000117 bp) in 2.099 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287949, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (240.02, 119.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676282 reads in 216.701 CPU sec, 6.929 real sec
[M::mem_process_seqs] Processed 676042 reads in 120.032 CPU sec, 3.810 real sec
sorting block 30, #reads = 1081693 mem = 536877399 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287598, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.46, 110.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676950 sequences (100000074 bp) in 2.930 CPU sec, 3.171 real sec...
[M::process] read 677428 sequences (100000211 bp) in 2.010 CPU sec, 2.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287517, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.49, 112.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677054 reads in 230.646 CPU sec, 7.384 real sec
[M::mem_process_seqs] Processed 676828 reads in 128.913 CPU sec, 4.124 real sec
sorting block 31, #reads = 1081971 mem = 536878922 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287534, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.18, 112.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677110 sequences (100000022 bp) in 3.173 CPU sec, 3.464 real sec...
[M::process] read 677136 sequences (100000011 bp) in 2.300 CPU sec, 2.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288244, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.12, 106.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676950 reads in 237.343 CPU sec, 7.611 real sec
[M::mem_process_seqs] Processed 677428 reads in 131.905 CPU sec, 4.201 real sec
sorting block 32, #reads = 1082143 mem = 536879948 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287772, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.50, 110.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1082555 mem = 536878747 peak = 981065728
[M::process] read 677040 sequences (100000001 bp) in 3.581 CPU sec, 3.971 real sec...
[M::process] read 676946 sequences (100000262 bp) in 2.250 CPU sec, 2.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287786, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.27, 107.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677110 reads in 236.308 CPU sec, 7.536 real sec
[M::mem_process_seqs] Processed 677136 reads in 131.493 CPU sec, 4.178 real sec
sorting block 34, #reads = 1082600 mem = 536881170 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287620, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (225.05, 107.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676832 sequences (100000004 bp) in 3.258 CPU sec, 3.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287978, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.50, 108.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (63, 100, 760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2154)
[M::mem_pestat] mean and std.dev: (290.36, 345.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2851)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676160 sequences (100000074 bp) in 2.210 CPU sec, 2.216 real sec...
[M::mem_process_seqs] Processed 677040 reads in 225.318 CPU sec, 7.192 real sec
[M::mem_process_seqs] Processed 676946 reads in 124.561 CPU sec, 3.945 real sec
sorting block 35, #reads = 1082402 mem = 536879655 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288067, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.24, 110.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676410 sequences (100000193 bp) in 3.386 CPU sec, 3.678 real sec...
[M::process] read 676504 sequences (100000250 bp) in 2.070 CPU sec, 2.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287517, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.02, 121.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 218, 618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1802)
[M::mem_pestat] mean and std.dev: (306.64, 289.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2394)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676832 reads in 223.693 CPU sec, 7.136 real sec
[M::mem_process_seqs] Processed 676160 reads in 123.579 CPU sec, 3.934 real sec
sorting block 36, #reads = 1082431 mem = 536877650 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287588, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.43, 116.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 21, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 189)
[M::mem_pestat] mean and std.dev: (39.00, 37.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 247)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676872 sequences (100000133 bp) in 3.128 CPU sec, 3.410 real sec...
[M::process] read 676974 sequences (100000145 bp) in 2.079 CPU sec, 2.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287248, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.75, 119.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676410 reads in 229.759 CPU sec, 7.334 real sec
[M::mem_process_seqs] Processed 676504 reads in 130.449 CPU sec, 4.155 real sec
sorting block 37, #reads = 1082500 mem = 536879953 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287344, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.09, 110.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1080870 mem = 536876446 peak = 981065728
[M::process] read 676990 sequences (100000271 bp) in 3.302 CPU sec, 3.580 real sec...
[M::process] read 676978 sequences (100000266 bp) in 2.180 CPU sec, 2.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287608, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.41, 110.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676872 reads in 225.802 CPU sec, 7.201 real sec
[M::mem_process_seqs] Processed 676974 reads in 124.001 CPU sec, 3.937 real sec
sorting block 39, #reads = 1081841 mem = 536882454 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287650, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.25, 109.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 48, 89)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259)
[M::mem_pestat] mean and std.dev: (39.25, 37.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 344)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677008 sequences (100000264 bp) in 3.117 CPU sec, 3.384 real sec...
[M::process] read 676948 sequences (100000034 bp) in 1.540 CPU sec, 1.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288182, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.89, 107.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (65, 403, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1571)
[M::mem_pestat] mean and std.dev: (255.82, 216.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2073)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676990 reads in 214.073 CPU sec, 6.848 real sec
[M::mem_process_seqs] Processed 676978 reads in 113.468 CPU sec, 3.611 real sec
sorting block 40, #reads = 1081893 mem = 536881147 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288196, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.90, 106.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677100 sequences (100000024 bp) in 3.566 CPU sec, 3.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288138, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.66, 107.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677012 sequences (100000030 bp) in 2.170 CPU sec, 2.174 real sec...
[M::mem_process_seqs] Processed 677008 reads in 210.659 CPU sec, 6.700 real sec
[M::mem_process_seqs] Processed 676948 reads in 117.149 CPU sec, 3.711 real sec
sorting block 41, #reads = 1082200 mem = 536879803 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287976, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.33, 107.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677016 sequences (100000081 bp) in 2.842 CPU sec, 3.171 real sec...
[M::process] read 676544 sequences (100000296 bp) in 2.119 CPU sec, 2.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287841, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.79, 109.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677100 reads in 209.341 CPU sec, 6.691 real sec
[M::mem_process_seqs] Processed 677012 reads in 118.746 CPU sec, 3.775 real sec
sorting block 42, #reads = 1082380 mem = 536879049 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287742, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.31, 110.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 43, #reads = 1082119 mem = 536878712 peak = 981065728
[M::process] read 676792 sequences (100000232 bp) in 3.367 CPU sec, 3.719 real sec...
[M::process] read 676444 sequences (100000127 bp) in 1.490 CPU sec, 1.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287254, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.71, 118.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677016 reads in 211.952 CPU sec, 6.769 real sec
[M::mem_process_seqs] Processed 676544 reads in 116.490 CPU sec, 3.714 real sec
sorting block 44, #reads = 1082251 mem = 536878545 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287294, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.41, 112.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 448, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1264)
[M::mem_pestat] mean and std.dev: (284.10, 232.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1672)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676876 sequences (100000034 bp) in 2.984 CPU sec, 3.192 real sec...
[M::process] read 676884 sequences (100000179 bp) in 2.089 CPU sec, 2.088 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286998, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.01, 116.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 40, 1174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3492)
[M::mem_pestat] mean and std.dev: (565.92, 584.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4651)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676792 reads in 211.791 CPU sec, 6.754 real sec
[M::mem_process_seqs] Processed 676444 reads in 116.451 CPU sec, 3.690 real sec
sorting block 45, #reads = 1082193 mem = 536878921 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287828, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.24, 109.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 79, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1326)
[M::mem_pestat] mean and std.dev: (165.89, 200.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1765)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676756 sequences (100000124 bp) in 3.385 CPU sec, 3.647 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287628, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.21, 111.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676824 sequences (100000081 bp) in 2.140 CPU sec, 2.143 real sec...
[M::mem_process_seqs] Processed 676876 reads in 200.638 CPU sec, 6.373 real sec
[M::mem_process_seqs] Processed 676884 reads in 111.829 CPU sec, 3.541 real sec
sorting block 46, #reads = 1082062 mem = 536878157 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287582, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.47, 113.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677044 sequences (100000002 bp) in 2.929 CPU sec, 3.167 real sec...
[M::process] read 676996 sequences (100000206 bp) in 2.070 CPU sec, 2.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287790, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.37, 111.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676756 reads in 199.671 CPU sec, 6.357 real sec
[M::mem_process_seqs] Processed 676824 reads in 111.637 CPU sec, 3.539 real sec
sorting block 47, #reads = 1082064 mem = 536875957 peak = 981065728
sorting block 48, #reads = 1082076 mem = 536879278 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288210, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.14, 105.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 100, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (157.22, 193.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677146 sequences (100000262 bp) in 3.281 CPU sec, 3.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288067, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.54, 107.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677076 sequences (100000174 bp) in 2.050 CPU sec, 2.052 real sec...
[M::mem_process_seqs] Processed 677044 reads in 203.929 CPU sec, 6.494 real sec
[M::mem_process_seqs] Processed 676996 reads in 116.586 CPU sec, 3.700 real sec
sorting block 49, #reads = 1082618 mem = 536880828 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288123, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.90, 108.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676850 sequences (100000003 bp) in 2.642 CPU sec, 2.930 real sec...
[M::process] read 676830 sequences (100000139 bp) in 2.070 CPU sec, 2.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287968, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.53, 110.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677146 reads in 202.260 CPU sec, 6.458 real sec
[M::mem_process_seqs] Processed 677076 reads in 113.417 CPU sec, 3.603 real sec
sorting block 50, #reads = 1082421 mem = 536881686 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287856, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.98, 110.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676800 sequences (100000296 bp) in 3.370 CPU sec, 3.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287854, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676750 sequences (100000225 bp) in 2.110 CPU sec, 2.108 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.83, 112.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676850 reads in 203.414 CPU sec, 6.464 real sec
[M::mem_process_seqs] Processed 676830 reads in 113.537 CPU sec, 3.600 real sec
sorting block 51, #reads = 1082501 mem = 536878901 peak = 981065728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287525, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.92, 113.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676770 sequences (100000055 bp) in 3.575 CPU sec, 3.972 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288420, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.39, 113.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676800 reads in 199.817 CPU sec, 6.360 real sec
[M::mem_process_seqs] Processed 676750 reads in 110.415 CPU sec, 3.493 real sec
sorting block 52, #reads = 1082120 mem = 536877349 peak = 981065728
[M::process] read 677428 sequences (100000190 bp) in 2.376 CPU sec, 2.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288335, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.34, 114.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1082263 mem = 536881080 peak = 993878016
[M::process] read 677086 sequences (100000009 bp) in 3.350 CPU sec, 3.662 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288231, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.41, 106.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676770 reads in 198.369 CPU sec, 6.320 real sec
[M::mem_process_seqs] Processed 677428 reads in 113.002 CPU sec, 3.569 real sec
[M::process] read 676770 sequences (100000096 bp) in 2.336 CPU sec, 2.418 real sec...
sorting block 54, #reads = 1082377 mem = 536878988 peak = 993878016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288036, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.53, 113.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676936 sequences (100000056 bp) in 2.994 CPU sec, 3.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287521, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.87, 114.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677086 reads in 199.336 CPU sec, 6.369 real sec
[M::mem_process_seqs] Processed 676770 reads in 112.833 CPU sec, 3.575 real sec
[M::process] read 677268 sequences (100000166 bp) in 2.279 CPU sec, 2.367 real sec...
sorting block 55, #reads = 1082374 mem = 536878567 peak = 993878016
[M::process] read 677172 sequences (100000239 bp) in 3.168 CPU sec, 3.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288098, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.75, 103.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 26, 54)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 158)
[M::mem_pestat] mean and std.dev: (16.62, 17.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 210)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676948 sequences (100000080 bp) in 1.400 CPU sec, 1.399 real sec...
[M::mem_process_seqs] Processed 677268 reads in 94.935 CPU sec, 2.987 real sec
sorting block 56, #reads = 1082216 mem = 536877792 peak = 993878016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287725, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.28, 109.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288560, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.46, 104.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676740 sequences (100000112 bp) in 2.221 CPU sec, 2.224 real sec...
[M::mem_process_seqs] Processed 676936 reads in 281.446 CPU sec, 8.950 real sec
[M::mem_process_seqs] Processed 677172 reads in 107.508 CPU sec, 3.417 real sec
sorting block 57, #reads = 1082282 mem = 536881101 peak = 993878016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287913, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.43, 109.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 102, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1951)
[M::mem_pestat] mean and std.dev: (335.00, 375.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2586)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676848 sequences (100000189 bp) in 3.192 CPU sec, 3.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287903, 16, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.07, 111.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 89, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1976)
[M::mem_pestat] mean and std.dev: (172.38, 230.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2622)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676948 reads in 185.728 CPU sec, 5.909 real sec
[M::mem_process_seqs] Processed 676740 reads in 100.650 CPU sec, 3.176 real sec
[M::process] read 676730 sequences (100000293 bp) in 2.120 CPU sec, 2.162 real sec...
sorting block 58, #reads = 1082806 mem = 536877266 peak = 993878016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288155, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.44, 113.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676642 sequences (100000286 bp) in 2.714 CPU sec, 2.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287797, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.01, 114.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676622 sequences (100000247 bp) in 1.990 CPU sec, 1.996 real sec...
[M::mem_process_seqs] Processed 676848 reads in 187.916 CPU sec, 5.989 real sec
[M::mem_process_seqs] Processed 676730 reads in 108.033 CPU sec, 3.425 real sec
sorting block 59, #reads = 1082118 mem = 536881662 peak = 993878016
[M::process] read 182444 sequences (26969681 bp) in 1.166 CPU sec, 1.279 real sec...
sorting block 60, #reads = 1082301 mem = 536880006 peak = 993878016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287496, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.35, 115.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287631, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.26, 117.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676642 reads in 191.840 CPU sec, 6.229 real sec
[M::mem_process_seqs] Processed 676622 reads in 103.318 CPU sec, 3.353 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 77675, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.98, 116.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 182444 reads in 25.741 CPU sec, 0.880 real sec
sorting block 61, #reads = 1082102 mem = 536879973 peak = 993878016
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67182966
overall: 69543333888 mem 12999.664 user 635.408 sys 923.384 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L005\tPU:L005\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.T21-D0003376_R1.fastq.gz 0002.T21-D0003376_R2.fastq.gz
[main] Real time: 923.413 sec; CPU: 13635.076 sec
sorting block 62, #reads = 605176 mem = 300339475 peak = 993878016
uniq header count 1
merging 63 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2621404183
output reads: 67703555
bam_mem_sort: 63 calls 41.380 user 0.780 sys 42.251 real
bam_write: 63 calls 19.937 user 0.948 sys 20.838 real
execute: 1 calls 13.017 user 0.554 sys 938.102 real
merge_files: 1 calls 12.996 user 0.527 sys 14.148 real
parse_chunk: 1001 calls 44.589 user 12.045 sys 57.348 real
read_chunk: 2696 calls 24.528 user 22.154 sys 362.467 real
sort_block: 1 calls 21.121 user 21.666 sys 354.029 real
write_chunk: 1693 calls 12.686 user 0.354 sys 13.395 real
overall: 2336382976 mem 623.300 user 44.755 sys 938.119 real
ls: cannot access '*.cram': No such file or directory