.command.log - Workdir - DAQ:SENTIEON_BWAMEM (T21-D0003376;L008;0002)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a4/cb51497c311f66d24a1cf38090eb9e/.command.log
Size: 92.5 KB
Task: DAQ:SENTIEON_BWAMEM (T21-D0003376;L008;0002)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a4/cb51497c311f66d24a1cf38090eb9e/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e8/b8145cebcda724984d019f97962525/T21-D0003376/0002.T21-D0003376_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a4/cb51497c311f66d24a1cf38090eb9e/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e8/b8145cebcda724984d019f97962525/T21-D0003376/0002.T21-D0003376_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2605.550 CPU sec, 89.380 real sec
[M::process] read 677566 sequences (100000286 bp) in 1.394 CPU sec, 1.404 real sec...
[M::process] read 677520 sequences (100000057 bp) in 1.700 CPU sec, 1.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288878, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.29, 98.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677246 sequences (100000269 bp) in 1.770 CPU sec, 1.800 real sec...
[M::process] read 677106 sequences (100000158 bp) in 1.834 CPU sec, 1.855 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288360, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.39, 100.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 16, 16)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 26)
[M::mem_pestat] mean and std.dev: (11.88, 4.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 31)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677566 reads in 212.006 CPU sec, 6.813 real sec
[M::mem_process_seqs] Processed 677520 reads in 122.757 CPU sec, 4.083 real sec
sorting block 0, #reads = 1082468 mem = 536878204 peak = 525119488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288756, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (222.07, 102.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676946 sequences (100000097 bp) in 2.527 CPU sec, 2.819 real sec...
[M::process] read 676800 sequences (100000222 bp) in 1.521 CPU sec, 1.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288213, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (225.09, 106.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677246 reads in 211.464 CPU sec, 6.913 real sec
[M::mem_process_seqs] Processed 677106 reads in 120.392 CPU sec, 3.949 real sec
sorting block 1, #reads = 1082523 mem = 536879662 peak = 650948608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287996, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (229.03, 110.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676780 sequences (100000295 bp) in 3.060 CPU sec, 3.385 real sec...
[M::process] read 676450 sequences (100000160 bp) in 1.785 CPU sec, 1.795 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288372, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.57, 110.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 34, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1926)
[M::mem_pestat] mean and std.dev: (223.42, 356.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2561)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676946 reads in 212.672 CPU sec, 6.796 real sec
[M::mem_process_seqs] Processed 676800 reads in 119.905 CPU sec, 3.833 real sec
sorting block 2, #reads = 1082025 mem = 536879092 peak = 668090368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288135, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.95, 113.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676420 sequences (100000044 bp) in 3.159 CPU sec, 3.558 real sec...
[M::process] read 676160 sequences (100000159 bp) in 1.727 CPU sec, 1.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287517, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.06, 117.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676780 reads in 216.465 CPU sec, 6.941 real sec
sorting block 3, #reads = 1082378 mem = 536878890 peak = 687030272
[M::process] read 676200 sequences (100000240 bp) in 1.791 CPU sec, 1.793 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287578, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.83, 116.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676420 reads in 120.837 CPU sec, 3.884 real sec
sorting block 4, #reads = 1082287 mem = 536877359 peak = 688312320
[M::mem_process_seqs] Processed 676450 reads in 294.094 CPU sec, 9.467 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287856, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.65, 117.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676090 sequences (100000144 bp) in 2.445 CPU sec, 2.726 real sec...
[M::process] read 676628 sequences (100000184 bp) in 1.766 CPU sec, 1.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287748, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.33, 118.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 462, 658)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1906)
[M::mem_pestat] mean and std.dev: (454.36, 486.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2530)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676160 reads in 247.578 CPU sec, 7.974 real sec
[M::mem_process_seqs] Processed 676200 reads in 140.201 CPU sec, 4.550 real sec
sorting block 5, #reads = 1081783 mem = 536877896 peak = 688312320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288025, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (240.08, 120.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677586 sequences (100000001 bp) in 2.578 CPU sec, 2.864 real sec...
[M::process] read 677374 sequences (100000247 bp) in 1.672 CPU sec, 1.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288447, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.84, 113.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676090 reads in 239.319 CPU sec, 7.774 real sec
sorting block 6, #reads = 1081845 mem = 536878820 peak = 750030848
[M::process] read 677176 sequences (100000262 bp) in 1.671 CPU sec, 1.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289137, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (219.00, 98.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677586 reads in 132.055 CPU sec, 4.278 real sec
sorting block 7, #reads = 1082289 mem = 536875518 peak = 783687680
[M::mem_process_seqs] Processed 676628 reads in 319.337 CPU sec, 10.417 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288865, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.44, 101.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677076 sequences (100000200 bp) in 2.506 CPU sec, 2.946 real sec...
[M::process] read 676942 sequences (100000257 bp) in 1.727 CPU sec, 1.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288595, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (223.98, 104.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 58, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1900)
[M::mem_pestat] mean and std.dev: (224.79, 271.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2522)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677374 reads in 256.139 CPU sec, 8.322 real sec
sorting block 8, #reads = 1082390 mem = 536879346 peak = 783687680
[M::process] read 676820 sequences (100000154 bp) in 1.572 CPU sec, 1.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288716, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.56, 107.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677076 reads in 128.330 CPU sec, 4.157 real sec
sorting block 9, #reads = 1083119 mem = 536878470 peak = 783687680
[M::mem_process_seqs] Processed 677176 reads in 320.530 CPU sec, 10.391 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288663, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.83, 110.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 12, 57)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 149)
[M::mem_pestat] mean and std.dev: (23.54, 32.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 195)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676644 sequences (100000078 bp) in 2.638 CPU sec, 2.971 real sec...
[M::process] read 676584 sequences (100000151 bp) in 1.419 CPU sec, 1.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288531, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.64, 111.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676942 reads in 256.161 CPU sec, 8.316 real sec
[M::mem_process_seqs] Processed 676820 reads in 140.731 CPU sec, 4.593 real sec
sorting block 10, #reads = 1082756 mem = 536877976 peak = 783687680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287994, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.35, 114.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676562 sequences (100000279 bp) in 2.416 CPU sec, 2.813 real sec...
[M::process] read 676476 sequences (100000243 bp) in 1.540 CPU sec, 1.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288385, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.04, 116.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676644 reads in 243.819 CPU sec, 7.971 real sec
sorting block 11, #reads = 1082613 mem = 536878605 peak = 783687680
[M::mem_process_seqs] Processed 676584 reads in 139.135 CPU sec, 4.514 real sec
sorting block 12, #reads = 1082409 mem = 536879492 peak = 783687680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288345, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.86, 116.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 51, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (139.70, 129.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676218 sequences (100000037 bp) in 2.752 CPU sec, 3.086 real sec...
[M::process] read 676362 sequences (100000270 bp) in 1.720 CPU sec, 1.726 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288387, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.32, 118.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 63, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (139.33, 203.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676562 reads in 239.517 CPU sec, 7.775 real sec
[M::mem_process_seqs] Processed 676476 reads in 135.891 CPU sec, 4.434 real sec
sorting block 13, #reads = 1082446 mem = 536879092 peak = 783687680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288192, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.78, 120.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677290 sequences (100000175 bp) in 2.783 CPU sec, 3.097 real sec...
[M::process] read 677462 sequences (100000096 bp) in 1.514 CPU sec, 1.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288450, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.41, 120.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 116, 740)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2138)
[M::mem_pestat] mean and std.dev: (274.90, 307.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2837)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676218 reads in 240.156 CPU sec, 7.822 real sec
[M::mem_process_seqs] Processed 676362 reads in 138.847 CPU sec, 4.563 real sec
sorting block 14, #reads = 1082005 mem = 536878827 peak = 783687680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288636, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.52, 105.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677098 sequences (100000206 bp) in 3.317 CPU sec, 3.748 real sec...
[M::process] read 677082 sequences (100000214 bp) in 1.614 CPU sec, 1.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288839, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.97, 100.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677290 reads in 243.029 CPU sec, 7.871 real sec
[M::mem_process_seqs] Processed 677462 reads in 141.761 CPU sec, 4.611 real sec
sorting block 15, #reads = 1082201 mem = 536878540 peak = 783687680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288640, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.75, 102.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676954 sequences (100000022 bp) in 2.688 CPU sec, 2.997 real sec...
sorting block 16, #reads = 1083166 mem = 536881180 peak = 783687680
[M::process] read 676726 sequences (100000217 bp) in 1.636 CPU sec, 1.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288575, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (225.21, 105.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 79, 628)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1856)
[M::mem_pestat] mean and std.dev: (260.17, 294.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2470)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677098 reads in 242.481 CPU sec, 7.890 real sec
[M::mem_process_seqs] Processed 677082 reads in 139.161 CPU sec, 4.598 real sec
sorting block 17, #reads = 1082409 mem = 536881653 peak = 783687680
[M::process] read 676664 sequences (100000111 bp) in 2.349 CPU sec, 2.610 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288407, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.45, 109.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676628 sequences (100000165 bp) in 1.706 CPU sec, 1.721 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288437, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.10, 110.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 10, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268)
[M::mem_pestat] mean and std.dev: (18.55, 26.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 357)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676954 reads in 243.791 CPU sec, 7.963 real sec
[M::mem_process_seqs] Processed 676726 reads in 137.765 CPU sec, 4.550 real sec
sorting block 18, #reads = 1082488 mem = 536878833 peak = 783687680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288390, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.98, 113.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676472 sequences (100000142 bp) in 3.213 CPU sec, 3.632 real sec...
[M::process] read 676396 sequences (100000039 bp) in 1.466 CPU sec, 1.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288226, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.19, 115.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676664 reads in 245.807 CPU sec, 8.069 real sec
[M::mem_process_seqs] Processed 676628 reads in 139.365 CPU sec, 4.617 real sec
sorting block 19, #reads = 1082488 mem = 536879162 peak = 783687680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288147, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.48, 117.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 18, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1945)
[M::mem_pestat] mean and std.dev: (86.18, 181.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2590)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676286 sequences (100000174 bp) in 2.705 CPU sec, 3.165 real sec...
[M::process] read 676252 sequences (100000028 bp) in 1.609 CPU sec, 1.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287589, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.03, 117.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676472 reads in 247.035 CPU sec, 8.091 real sec
[M::mem_process_seqs] Processed 676396 reads in 142.200 CPU sec, 4.663 real sec
sorting block 20, #reads = 1082503 mem = 536879293 peak = 783687680
[M::process] read 676186 sequences (100000232 bp) in 2.390 CPU sec, 2.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287720, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.15, 118.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1082102 mem = 536877941 peak = 811999232
[M::process] read 677576 sequences (100000210 bp) in 1.598 CPU sec, 1.663 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288131, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.04, 119.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676286 reads in 241.671 CPU sec, 7.982 real sec
[M::mem_process_seqs] Processed 676252 reads in 136.670 CPU sec, 4.575 real sec
sorting block 22, #reads = 1081975 mem = 536879712 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287568, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.65, 119.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (49, 107, 188)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (102.10, 64.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 605)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677302 sequences (100000072 bp) in 2.823 CPU sec, 3.152 real sec...
[M::process] read 677304 sequences (100000001 bp) in 1.621 CPU sec, 1.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288646, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (217.05, 95.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676186 reads in 242.164 CPU sec, 7.887 real sec
[M::mem_process_seqs] Processed 677576 reads in 138.056 CPU sec, 4.532 real sec
sorting block 23, #reads = 1081715 mem = 536878036 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288553, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.79, 98.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677134 sequences (100000239 bp) in 2.634 CPU sec, 2.979 real sec...
[M::process] read 676890 sequences (100000024 bp) in 1.671 CPU sec, 1.706 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287921, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (222.30, 102.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677302 reads in 242.963 CPU sec, 7.929 real sec
[M::mem_process_seqs] Processed 677304 reads in 139.284 CPU sec, 4.572 real sec
sorting block 24, #reads = 1083120 mem = 536878525 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288444, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.75, 104.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676696 sequences (100000234 bp) in 2.476 CPU sec, 2.845 real sec...
[M::process] read 676494 sequences (100000221 bp) in 1.591 CPU sec, 1.593 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288154, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.39, 108.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 73, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (165.36, 204.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677134 reads in 244.673 CPU sec, 7.968 real sec
[M::mem_process_seqs] Processed 676890 reads in 140.732 CPU sec, 4.583 real sec
sorting block 25, #reads = 1082364 mem = 536877511 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288096, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.35, 111.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (57, 100, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146)
[M::mem_pestat] mean and std.dev: (303.73, 335.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1644)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 26, #reads = 1082498 mem = 536879022 peak = 811999232
[M::process] read 676418 sequences (100000233 bp) in 2.847 CPU sec, 3.202 real sec...
[M::process] read 676388 sequences (100000222 bp) in 1.560 CPU sec, 1.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287667, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.28, 112.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676696 reads in 245.588 CPU sec, 7.972 real sec
[M::mem_process_seqs] Processed 676494 reads in 140.254 CPU sec, 4.567 real sec
sorting block 27, #reads = 1081783 mem = 536881206 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287676, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (235.24, 115.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676272 sequences (100000071 bp) in 2.954 CPU sec, 3.419 real sec...
[M::process] read 676356 sequences (100000268 bp) in 1.481 CPU sec, 1.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287844, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.25, 116.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 418, 660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1934)
[M::mem_pestat] mean and std.dev: (259.70, 278.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2571)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676418 reads in 247.306 CPU sec, 8.138 real sec
[M::mem_process_seqs] Processed 676388 reads in 138.961 CPU sec, 4.634 real sec
sorting block 28, #reads = 1081821 mem = 536880419 peak = 811999232
[M::process] read 676148 sequences (100000154 bp) in 2.645 CPU sec, 3.006 real sec...
[M::process] read 676212 sequences (100000192 bp) in 1.557 CPU sec, 1.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287800, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.63, 118.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676356 reads in 128.084 CPU sec, 4.167 real sec
sorting block 29, #reads = 1081961 mem = 536879596 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287455, 19, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (237.47, 117.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 7, 45)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 129)
[M::mem_pestat] mean and std.dev: (17.88, 20.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 171)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677688 sequences (100000030 bp) in 1.601 CPU sec, 1.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287534, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (239.02, 118.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 187, 191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (101.00, 89.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676272 reads in 375.773 CPU sec, 12.262 real sec
[M::mem_process_seqs] Processed 676148 reads in 153.607 CPU sec, 5.074 real sec
sorting block 30, #reads = 1081784 mem = 536878256 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288281, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.38, 121.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677664 sequences (100000082 bp) in 2.898 CPU sec, 3.403 real sec...
[M::process] read 677318 sequences (100000195 bp) in 1.880 CPU sec, 1.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288791, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (211.42, 89.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 645)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676212 reads in 250.244 CPU sec, 8.229 real sec
[M::mem_process_seqs] Processed 677688 reads in 144.359 CPU sec, 4.722 real sec
sorting block 31, #reads = 1082315 mem = 536877954 peak = 811999232
[M::process] read 677236 sequences (100000018 bp) in 2.152 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288643, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546)
[M::mem_pestat] mean and std.dev: (214.97, 93.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 674)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677192 sequences (100000235 bp) in 1.596 CPU sec, 1.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288212, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.28, 101.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677664 reads in 249.390 CPU sec, 8.143 real sec
[M::mem_process_seqs] Processed 677318 reads in 142.741 CPU sec, 4.702 real sec
sorting block 32, #reads = 1082647 mem = 536877000 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288623, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (223.70, 104.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677094 sequences (100000114 bp) in 2.682 CPU sec, 2.972 real sec...
sorting block 33, #reads = 1082683 mem = 536877364 peak = 811999232
[M::process] read 676916 sequences (100000148 bp) in 1.799 CPU sec, 1.849 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287743, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.41, 107.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677236 reads in 251.719 CPU sec, 8.261 real sec
[M::mem_process_seqs] Processed 677192 reads in 142.982 CPU sec, 4.701 real sec
sorting block 34, #reads = 1082406 mem = 536879837 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288026, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.65, 106.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676754 sequences (100000255 bp) in 2.784 CPU sec, 3.238 real sec...
[M::process] read 676686 sequences (100000231 bp) in 1.654 CPU sec, 1.673 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287989, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.36, 109.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 28, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (76.85, 103.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677094 reads in 250.262 CPU sec, 8.159 real sec
[M::mem_process_seqs] Processed 676916 reads in 144.804 CPU sec, 4.751 real sec
sorting block 35, #reads = 1082609 mem = 536878885 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288166, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.88, 113.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676714 sequences (100000173 bp) in 2.648 CPU sec, 3.016 real sec...
[M::process] read 676718 sequences (100000020 bp) in 1.781 CPU sec, 1.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288138, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.96, 112.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676754 reads in 248.330 CPU sec, 8.139 real sec
[M::mem_process_seqs] Processed 676686 reads in 143.184 CPU sec, 4.744 real sec
sorting block 36, #reads = 1082195 mem = 536879003 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288183, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.39, 114.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676720 sequences (100000141 bp) in 2.565 CPU sec, 3.087 real sec...
[M::process] read 677290 sequences (100000067 bp) in 1.483 CPU sec, 1.568 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287895, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.20, 114.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676714 reads in 253.855 CPU sec, 8.284 real sec
[M::mem_process_seqs] Processed 676718 reads in 144.536 CPU sec, 4.726 real sec
sorting block 37, #reads = 1082671 mem = 536879257 peak = 811999232
[M::process] read 677882 sequences (100000203 bp) in 2.639 CPU sec, 2.898 real sec...
sorting block 38, #reads = 1082180 mem = 536879731 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287706, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.02, 115.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 38, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1613)
[M::mem_pestat] mean and std.dev: (265.00, 384.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2145)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677654 sequences (100000174 bp) in 1.749 CPU sec, 1.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288415, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (221.59, 103.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 18, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 159)
[M::mem_pestat] mean and std.dev: (22.50, 15.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 208)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676720 reads in 247.552 CPU sec, 8.086 real sec
[M::mem_process_seqs] Processed 677290 reads in 134.005 CPU sec, 4.503 real sec
sorting block 39, #reads = 1081727 mem = 536880435 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289135, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (206.99, 83.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 609)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677356 sequences (100000048 bp) in 2.628 CPU sec, 3.007 real sec...
[M::process] read 677160 sequences (100000213 bp) in 1.736 CPU sec, 1.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288985, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (213.57, 92.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677882 reads in 242.864 CPU sec, 7.927 real sec
[M::mem_process_seqs] Processed 677654 reads in 140.101 CPU sec, 4.598 real sec
sorting block 40, #reads = 1082994 mem = 536878195 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288681, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.48, 104.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677172 sequences (100000044 bp) in 2.849 CPU sec, 3.130 real sec...
[M::process] read 677152 sequences (100000192 bp) in 1.829 CPU sec, 1.843 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288510, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.42, 105.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677356 reads in 247.253 CPU sec, 8.033 real sec
[M::mem_process_seqs] Processed 677160 reads in 143.774 CPU sec, 4.690 real sec
sorting block 41, #reads = 1082654 mem = 536878281 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288485, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (224.98, 106.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676872 sequences (100000141 bp) in 2.535 CPU sec, 2.977 real sec...
[M::process] read 676832 sequences (100000228 bp) in 1.489 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288035, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.72, 108.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677172 reads in 248.414 CPU sec, 8.119 real sec
[M::mem_process_seqs] Processed 677152 reads in 142.264 CPU sec, 4.664 real sec
sorting block 42, #reads = 1082751 mem = 536878932 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288082, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.85, 109.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 18, 1136)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3374)
[M::mem_pestat] mean and std.dev: (348.33, 479.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4493)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 43, #reads = 1082702 mem = 536881657 peak = 811999232
[M::process] read 676918 sequences (100000001 bp) in 2.832 CPU sec, 3.178 real sec...
[M::process] read 676658 sequences (100000089 bp) in 1.902 CPU sec, 1.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288109, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.35, 111.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 136, 673)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1941)
[M::mem_pestat] mean and std.dev: (220.00, 248.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2575)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676872 reads in 252.488 CPU sec, 8.215 real sec
[M::mem_process_seqs] Processed 676832 reads in 146.957 CPU sec, 4.833 real sec
sorting block 44, #reads = 1081774 mem = 536879259 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288213, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.19, 112.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 28, 1004)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2956)
[M::mem_pestat] mean and std.dev: (604.73, 958.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4438)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676780 sequences (100000110 bp) in 2.530 CPU sec, 2.951 real sec...
[M::process] read 677172 sequences (100000263 bp) in 1.787 CPU sec, 1.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287689, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.55, 113.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676918 reads in 256.618 CPU sec, 8.381 real sec
[M::mem_process_seqs] Processed 676658 reads in 146.964 CPU sec, 4.825 real sec
sorting block 45, #reads = 1081716 mem = 536882142 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288049, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.76, 114.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678098 sequences (100000128 bp) in 2.619 CPU sec, 2.987 real sec...
[M::process] read 677876 sequences (100000134 bp) in 1.679 CPU sec, 1.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288203, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (222.95, 105.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676780 reads in 249.725 CPU sec, 8.170 real sec
[M::mem_process_seqs] Processed 677172 reads in 143.104 CPU sec, 4.753 real sec
sorting block 46, #reads = 1082128 mem = 536881094 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288800, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 175, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440)
[M::mem_pestat] mean and std.dev: (199.57, 72.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 533)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677520 sequences (100000294 bp) in 2.501 CPU sec, 2.893 real sec...
[M::process] read 677482 sequences (100000146 bp) in 1.583 CPU sec, 1.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288619, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (208.47, 85.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 625)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678098 reads in 245.425 CPU sec, 7.969 real sec
sorting block 47, #reads = 1082479 mem = 536879497 peak = 811999232
[M::mem_process_seqs] Processed 677876 reads in 138.873 CPU sec, 4.490 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288734, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (217.75, 98.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1082866 mem = 536881416 peak = 811999232
[M::process] read 677188 sequences (100000227 bp) in 2.908 CPU sec, 3.198 real sec...
[M::process] read 677126 sequences (100000252 bp) in 1.688 CPU sec, 1.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288521, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.31, 102.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677520 reads in 251.104 CPU sec, 8.193 real sec
[M::mem_process_seqs] Processed 677482 reads in 143.017 CPU sec, 4.710 real sec
sorting block 49, #reads = 1083164 mem = 536880625 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288109, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (223.75, 105.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677084 sequences (100000272 bp) in 2.741 CPU sec, 3.081 real sec...
[M::process] read 676948 sequences (100000273 bp) in 1.761 CPU sec, 1.769 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288305, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.67, 108.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677188 reads in 247.780 CPU sec, 8.005 real sec
[M::mem_process_seqs] Processed 677126 reads in 140.675 CPU sec, 4.532 real sec
sorting block 50, #reads = 1082784 mem = 536877849 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287484, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.64, 108.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 50, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (70.58, 79.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676924 sequences (100000080 bp) in 3.469 CPU sec, 3.791 real sec...
[M::process] read 676912 sequences (100000115 bp) in 1.840 CPU sec, 1.843 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287966, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.17, 109.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677084 reads in 257.551 CPU sec, 8.282 real sec
[M::mem_process_seqs] Processed 676948 reads in 153.612 CPU sec, 4.991 real sec
sorting block 51, #reads = 1082352 mem = 536879164 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287888, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.79, 111.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676844 sequences (100000254 bp) in 2.970 CPU sec, 3.328 real sec...
[M::process] read 676828 sequences (100000023 bp) in 1.750 CPU sec, 1.753 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287850, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.87, 112.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676924 reads in 241.999 CPU sec, 7.801 real sec
sorting block 52, #reads = 1082731 mem = 536880025 peak = 811999232
[M::mem_process_seqs] Processed 676912 reads in 133.952 CPU sec, 4.298 real sec
sorting block 53, #reads = 1082259 mem = 536878666 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288040, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.47, 112.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677942 sequences (100000208 bp) in 2.747 CPU sec, 2.955 real sec...
[M::process] read 677858 sequences (100000087 bp) in 1.848 CPU sec, 1.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288237, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (223.82, 106.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676844 reads in 227.958 CPU sec, 7.301 real sec
[M::mem_process_seqs] Processed 676828 reads in 127.088 CPU sec, 4.086 real sec
sorting block 54, #reads = 1082199 mem = 536878853 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288807, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 175, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449)
[M::mem_pestat] mean and std.dev: (200.55, 73.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 545)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677578 sequences (100000016 bp) in 2.823 CPU sec, 3.128 real sec...
[M::process] read 677512 sequences (100000089 bp) in 1.851 CPU sec, 1.858 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288784, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522)
[M::mem_pestat] mean and std.dev: (210.98, 89.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677942 reads in 220.574 CPU sec, 7.066 real sec
[M::mem_process_seqs] Processed 677858 reads in 126.919 CPU sec, 4.085 real sec
sorting block 55, #reads = 1082624 mem = 536878849 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288269, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546)
[M::mem_pestat] mean and std.dev: (214.98, 94.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 674)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 49, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1897)
[M::mem_pestat] mean and std.dev: (352.10, 476.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2518)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677306 sequences (100000134 bp) in 3.086 CPU sec, 3.365 real sec...
[M::process] read 677052 sequences (100000125 bp) in 1.810 CPU sec, 1.807 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288171, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.70, 100.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677578 reads in 228.044 CPU sec, 7.311 real sec
[M::mem_process_seqs] Processed 677512 reads in 127.981 CPU sec, 4.102 real sec
sorting block 56, #reads = 1082533 mem = 536881331 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287925, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.06, 103.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 91, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091)
[M::mem_pestat] mean and std.dev: (226.82, 230.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1431)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676954 sequences (100000196 bp) in 2.706 CPU sec, 2.951 real sec...
[M::process] read 676968 sequences (100000274 bp) in 1.560 CPU sec, 1.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288068, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.79, 108.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677306 reads in 213.955 CPU sec, 6.855 real sec
sorting block 57, #reads = 1082838 mem = 536879068 peak = 811999232
[M::mem_process_seqs] Processed 677052 reads in 119.912 CPU sec, 3.847 real sec
sorting block 58, #reads = 1082795 mem = 536879031 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287611, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.73, 108.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 49, 116)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320)
[M::mem_pestat] mean and std.dev: (47.27, 40.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 422)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676820 sequences (100000260 bp) in 2.804 CPU sec, 3.019 real sec...
[M::process] read 676738 sequences (100000110 bp) in 1.560 CPU sec, 1.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288006, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.98, 110.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676954 reads in 227.037 CPU sec, 7.281 real sec
[M::mem_process_seqs] Processed 676968 reads in 129.788 CPU sec, 4.206 real sec
sorting block 59, #reads = 1082274 mem = 536876612 peak = 811999232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287879, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.19, 112.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 17, 34)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 88)
[M::mem_pestat] mean and std.dev: (14.88, 9.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 115)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676648 sequences (100000137 bp) in 2.554 CPU sec, 2.879 real sec...
[M::process] read 676640 sequences (100000157 bp) in 1.216 CPU sec, 1.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287539, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.14, 114.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 51, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2079)
[M::mem_pestat] mean and std.dev: (292.60, 423.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2769)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676820 reads in 226.689 CPU sec, 7.296 real sec
[M::mem_process_seqs] Processed 676738 reads in 132.359 CPU sec, 4.288 real sec
sorting block 60, #reads = 1082587 mem = 536878901 peak = 811999232
[M::process] read 34272 sequences (5067522 bp) in 0.476 CPU sec, 0.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288457, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.80, 115.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 26, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213)
[M::mem_pestat] mean and std.dev: (25.56, 28.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 283)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287944, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.47, 115.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 14611, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.53, 120.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676648 reads in 211.498 CPU sec, 6.895 real sec
[M::mem_process_seqs] Processed 676640 reads in 131.304 CPU sec, 4.310 real sec
sorting block 61, #reads = 1082237 mem = 536878717 peak = 811999232
[M::mem_process_seqs] Processed 34272 reads in 132.028 CPU sec, 4.338 real sec
sorting block 62, #reads = 1082385 mem = 536878557 peak = 811999232
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67729134
overall: 69209042944 mem 14468.339 user 754.860 sys 511.590 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L008\tPU:L008\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.T21-D0003376_R1.fastq.gz 0002.T21-D0003376_R2.fastq.gz
[main] Real time: 511.621 sec; CPU: 15223.202 sec
sorting block 63, #reads = 65053 mem = 32294905 peak = 811999232
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2649616896
output reads: 68256026
bam_mem_sort: 64 calls 60.830 user 1.035 sys 63.043 real
bam_write: 64 calls 43.223 user 4.869 sys 75.312 real
execute: 1 calls 18.910 user 1.612 sys 532.980 real
merge_files: 1 calls 18.896 user 1.576 sys 21.250 real
parse_chunk: 1009 calls 49.933 user 14.257 sys 73.526 real
read_chunk: 2718 calls 35.566 user 17.626 sys 426.278 real
sort_block: 1 calls 27.182 user 16.822 sys 414.088 real
write_chunk: 1707 calls 18.466 user 1.338 sys 20.256 real
overall: 1970233344 mem 768.978 user 62.696 sys 532.997 real
ls: cannot access '*.cram': No such file or directory