.command.err - Workdir - DAQ:SENTIEON_BWAMEM (T21-D0003322;L002;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a8/e311b9f7859d3db1c7c041e9ed32de/.command.err
Size: 351.9 KB
Task: DAQ:SENTIEON_BWAMEM (T21-D0003322;L002;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003322.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1889.558 CPU sec, 65.023 real sec
[M::process] read 675172 sequences (100000209 bp) in 0.924 CPU sec, 0.932 real sec...
[M::process] read 675150 sequences (100000096 bp) in 1.508 CPU sec, 1.572 real sec...
[M::process] read 675192 sequences (100000133 bp) in 1.571 CPU sec, 1.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285973, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (248.37, 120.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674904 sequences (100000268 bp) in 1.457 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286095, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.93, 124.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 238, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (124.82, 105.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286000, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (251.22, 123.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675172 reads in 304.058 CPU sec, 9.898 real sec
[M::mem_process_seqs] Processed 675192 reads in 196.341 CPU sec, 6.459 real sec
[M::mem_process_seqs] Processed 675150 reads in 204.231 CPU sec, 6.740 real sec
sorting block 0, #reads = 1080527 mem = 536878486 peak = 612581376
[M::process] read 675026 sequences (100000252 bp) in 1.798 CPU sec, 1.888 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286037, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (252.30, 124.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 128, 3516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10524)
[M::mem_pestat] mean and std.dev: (1225.62, 1676.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14028)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675016 sequences (100000265 bp) in 1.577 CPU sec, 1.597 real sec...
[M::process] read 674990 sequences (100000025 bp) in 1.449 CPU sec, 1.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286309, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (252.21, 124.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674904 reads in 202.302 CPU sec, 6.458 real sec
sorting block 1, #reads = 1080267 mem = 536878302 peak = 897269760
[M::mem_process_seqs] Processed 675026 reads in 114.103 CPU sec, 3.643 real sec
sorting block 2, #reads = 1080068 mem = 536881312 peak = 956497920
[M::process] read 674866 sequences (100000061 bp) in 2.037 CPU sec, 2.114 real sec...
[M::process] read 674992 sequences (100000117 bp) in 1.386 CPU sec, 1.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286240, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.52, 127.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 73, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183)
[M::mem_pestat] mean and std.dev: (52.00, 26.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 238)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674990 reads in 104.595 CPU sec, 3.351 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286339, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (254.92, 126.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674942 sequences (100000126 bp) in 1.356 CPU sec, 1.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285257, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.96, 128.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 11, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (102.92, 160.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675016 reads in 310.444 CPU sec, 9.973 real sec
sorting block 3, #reads = 1080474 mem = 536877555 peak = 956497920
[M::process] read 674752 sequences (100000255 bp) in 1.290 CPU sec, 1.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285896, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.87, 128.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674992 reads in 109.400 CPU sec, 3.510 real sec
sorting block 4, #reads = 1080677 mem = 536877862 peak = 956497920
[M::mem_process_seqs] Processed 674866 reads in 354.406 CPU sec, 11.430 real sec
[M::process] read 674756 sequences (100000017 bp) in 2.028 CPU sec, 2.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285277, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.06, 129.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 241, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (169.73, 131.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674860 sequences (100000187 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285192, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.40, 131.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285842, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.86, 130.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674942 reads in 317.935 CPU sec, 10.208 real sec
[M::mem_process_seqs] Processed 674756 reads in 214.218 CPU sec, 6.904 real sec
[M::mem_process_seqs] Processed 674752 reads in 222.359 CPU sec, 7.197 real sec
sorting block 5, #reads = 1079422 mem = 536878345 peak = 992763904
[M::process] read 674806 sequences (100000215 bp) in 1.700 CPU sec, 1.822 real sec...
sorting block 6, #reads = 1079686 mem = 536880087 peak = 995983360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285295, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.39, 131.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 218, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (163.09, 161.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674570 sequences (100000290 bp) in 1.875 CPU sec, 2.057 real sec...
[M::process] read 674608 sequences (100000032 bp) in 1.319 CPU sec, 1.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285657, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.21, 131.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 145, 9679)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28985)
[M::mem_pestat] mean and std.dev: (2412.77, 4000.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38638)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674860 reads in 213.453 CPU sec, 6.920 real sec
[M::mem_process_seqs] Processed 674806 reads in 115.370 CPU sec, 3.699 real sec
sorting block 7, #reads = 1080211 mem = 536877864 peak = 995983360
[M::process] read 674594 sequences (100000186 bp) in 2.056 CPU sec, 2.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285738, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.76, 132.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674582 sequences (100000080 bp) in 1.440 CPU sec, 1.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286046, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.91, 133.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285107, 20, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.04, 133.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 192, 192)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (88.94, 91.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674570 reads in 306.037 CPU sec, 9.843 real sec
[M::mem_process_seqs] Processed 674608 reads in 214.052 CPU sec, 6.924 real sec
sorting block 8, #reads = 1080175 mem = 536880203 peak = 1011920896
[M::mem_process_seqs] Processed 674594 reads in 222.611 CPU sec, 7.232 real sec
sorting block 9, #reads = 1079722 mem = 536877688 peak = 1019097088
[M::process] read 674610 sequences (100000296 bp) in 1.962 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285220, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.53, 133.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 582, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1720)
[M::mem_pestat] mean and std.dev: (459.70, 429.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2289)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674470 sequences (100000287 bp) in 2.074 CPU sec, 2.210 real sec...
[M::process] read 674366 sequences (100000223 bp) in 1.445 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285259, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (263.22, 133.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 360, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (226.60, 218.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1348)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674582 reads in 221.627 CPU sec, 7.126 real sec
[M::mem_process_seqs] Processed 674610 reads in 122.877 CPU sec, 3.951 real sec
sorting block 10, #reads = 1080028 mem = 536879579 peak = 1019097088
[M::process] read 674424 sequences (100000271 bp) in 1.887 CPU sec, 1.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285404, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (265.06, 135.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674406 sequences (100000135 bp) in 1.488 CPU sec, 1.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285041, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.46, 137.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284652, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.54, 136.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674470 reads in 311.317 CPU sec, 10.005 real sec
sorting block 11, #reads = 1079965 mem = 536880048 peak = 1019097088
[M::mem_process_seqs] Processed 674424 reads in 217.163 CPU sec, 6.999 real sec
[M::mem_process_seqs] Processed 674366 reads in 225.784 CPU sec, 7.298 real sec
sorting block 12, #reads = 1079861 mem = 536878442 peak = 1020428288
[M::process] read 674230 sequences (100000181 bp) in 2.304 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285420, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.28, 137.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674262 sequences (100000211 bp) in 1.815 CPU sec, 1.906 real sec...
[M::process] read 674280 sequences (100000036 bp) in 1.435 CPU sec, 1.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284618, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.78, 137.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674406 reads in 220.211 CPU sec, 7.108 real sec
sorting block 13, #reads = 1079863 mem = 536877510 peak = 1020428288
[M::mem_process_seqs] Processed 674230 reads in 125.810 CPU sec, 4.022 real sec
sorting block 14, #reads = 1079902 mem = 536878146 peak = 1032884224
[M::process] read 674374 sequences (100000107 bp) in 2.163 CPU sec, 2.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285103, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.38, 136.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 66, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (136.67, 109.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674262 sequences (100000041 bp) in 1.550 CPU sec, 1.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284341, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.41, 138.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285507, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.55, 137.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674262 reads in 322.991 CPU sec, 10.405 real sec
[M::mem_process_seqs] Processed 674374 reads in 227.652 CPU sec, 7.341 real sec
[M::mem_process_seqs] Processed 674280 reads in 235.750 CPU sec, 7.621 real sec
sorting block 15, #reads = 1079954 mem = 536880049 peak = 1032884224
[M::process] read 674198 sequences (100000241 bp) in 2.154 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285586, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.37, 139.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1080397 mem = 536882014 peak = 1032884224
[M::process] read 674170 sequences (100000101 bp) in 1.980 CPU sec, 2.066 real sec...
[M::process] read 674174 sequences (100000171 bp) in 1.503 CPU sec, 1.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284794, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.92, 138.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674262 reads in 220.565 CPU sec, 7.108 real sec
[M::mem_process_seqs] Processed 674198 reads in 123.316 CPU sec, 3.973 real sec
sorting block 17, #reads = 1079481 mem = 536879872 peak = 1032884224
[M::process] read 674314 sequences (100000270 bp) in 2.204 CPU sec, 2.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285021, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.16, 139.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674424 sequences (100000095 bp) in 1.486 CPU sec, 1.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285045, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.22, 141.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 30, 1136)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3360)
[M::mem_pestat] mean and std.dev: (517.71, 640.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4472)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285363, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.23, 142.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674170 reads in 316.267 CPU sec, 10.184 real sec
[M::mem_process_seqs] Processed 674314 reads in 219.518 CPU sec, 7.075 real sec
[M::mem_process_seqs] Processed 674174 reads in 227.739 CPU sec, 7.359 real sec
sorting block 18, #reads = 1079900 mem = 536879442 peak = 1032884224
[M::process] read 674818 sequences (100000057 bp) in 2.217 CPU sec, 2.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284974, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.80, 140.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (55, 276, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1135)
[M::mem_pestat] mean and std.dev: (298.55, 232.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1495)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 19, #reads = 1079790 mem = 536882103 peak = 1032884224
[M::process] read 674912 sequences (100000113 bp) in 1.849 CPU sec, 1.962 real sec...
[M::process] read 674702 sequences (100000240 bp) in 1.462 CPU sec, 1.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285528, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.37, 132.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 161, 161)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479)
[M::mem_pestat] mean and std.dev: (75.38, 79.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 638)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674424 reads in 218.021 CPU sec, 7.027 real sec
[M::mem_process_seqs] Processed 674818 reads in 121.248 CPU sec, 3.906 real sec
sorting block 20, #reads = 1079965 mem = 536879843 peak = 1032884224
[M::process] read 674826 sequences (100000256 bp) in 2.122 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285106, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.52, 132.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674638 sequences (100000001 bp) in 1.438 CPU sec, 1.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285203, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.56, 133.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285401, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.59, 132.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674912 reads in 309.042 CPU sec, 9.942 real sec
[M::mem_process_seqs] Processed 674826 reads in 212.577 CPU sec, 6.853 real sec
[M::mem_process_seqs] Processed 674702 reads in 221.300 CPU sec, 7.158 real sec
sorting block 21, #reads = 1080388 mem = 536878599 peak = 1032884224
[M::process] read 674736 sequences (100000107 bp) in 2.196 CPU sec, 2.379 real sec...
sorting block 22, #reads = 1080249 mem = 536879711 peak = 1032884224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284973, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.23, 133.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674634 sequences (100000164 bp) in 1.934 CPU sec, 2.035 real sec...
[M::process] read 674746 sequences (100000240 bp) in 1.400 CPU sec, 1.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285623, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.61, 133.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674638 reads in 213.618 CPU sec, 6.865 real sec
[M::mem_process_seqs] Processed 674736 reads in 119.264 CPU sec, 3.800 real sec
sorting block 23, #reads = 1079732 mem = 536880401 peak = 1032884224
[M::process] read 674864 sequences (100000093 bp) in 2.105 CPU sec, 2.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285593, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.61, 133.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 4, 14)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 40)
[M::mem_pestat] mean and std.dev: (4.80, 5.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 53)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674528 sequences (100000007 bp) in 1.443 CPU sec, 1.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285495, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (259.87, 133.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 26, 626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1860)
[M::mem_pestat] mean and std.dev: (242.21, 307.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2477)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285378, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.17, 132.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 361, 788)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2344)
[M::mem_pestat] mean and std.dev: (392.90, 346.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3122)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674634 reads in 306.902 CPU sec, 9.888 real sec
[M::mem_process_seqs] Processed 674746 reads in 215.170 CPU sec, 6.936 real sec
[M::mem_process_seqs] Processed 674864 reads in 224.120 CPU sec, 7.247 real sec
sorting block 24, #reads = 1079942 mem = 536879590 peak = 1032884224
sorting block 25, #reads = 1080331 mem = 536878426 peak = 1032884224
[M::process] read 674570 sequences (100000285 bp) in 2.157 CPU sec, 2.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285249, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.70, 134.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674522 sequences (100000010 bp) in 2.032 CPU sec, 2.165 real sec...
[M::process] read 674370 sequences (100000298 bp) in 1.523 CPU sec, 1.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285853, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.44, 135.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 192, 927)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2397)
[M::mem_pestat] mean and std.dev: (271.29, 285.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3132)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674528 reads in 210.308 CPU sec, 6.739 real sec
[M::mem_process_seqs] Processed 674570 reads in 114.955 CPU sec, 3.666 real sec
sorting block 26, #reads = 1079984 mem = 536877726 peak = 1032884224
[M::process] read 674376 sequences (100000254 bp) in 1.831 CPU sec, 2.177 real sec...
[M::process] read 674544 sequences (100000224 bp) in 1.455 CPU sec, 1.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285029, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.34, 135.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674370 reads in 113.049 CPU sec, 3.713 real sec
sorting block 27, #reads = 1080172 mem = 536879102 peak = 1032884224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284764, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.59, 136.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674934 sequences (100000187 bp) in 1.455 CPU sec, 1.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285424, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.03, 135.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674522 reads in 316.138 CPU sec, 10.291 real sec
[M::mem_process_seqs] Processed 674376 reads in 224.749 CPU sec, 7.248 real sec
sorting block 28, #reads = 1079842 mem = 536880552 peak = 1032884224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285794, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.63, 135.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 197, 571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669)
[M::mem_pestat] mean and std.dev: (323.47, 318.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2218)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675134 sequences (100000007 bp) in 2.180 CPU sec, 2.522 real sec...
[M::process] read 675152 sequences (100000145 bp) in 1.539 CPU sec, 1.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286274, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.72, 127.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674544 reads in 200.207 CPU sec, 6.453 real sec
[M::mem_process_seqs] Processed 674934 reads in 112.108 CPU sec, 3.588 real sec
sorting block 29, #reads = 1079420 mem = 536879663 peak = 1032884224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286422, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 675096 sequences (100000218 bp) in 1.942 CPU sec, 2.282 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.73, 125.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 229, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085)
[M::mem_pestat] mean and std.dev: (256.50, 306.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1481)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674934 sequences (100000253 bp) in 1.424 CPU sec, 1.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286473, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (253.63, 125.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675134 reads in 201.156 CPU sec, 6.524 real sec
sorting block 30, #reads = 1080120 mem = 536877987 peak = 1075302400
[M::mem_process_seqs] Processed 675152 reads in 112.307 CPU sec, 3.609 real sec
sorting block 31, #reads = 1080610 mem = 536878899 peak = 1083731968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286245, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (253.21, 124.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674850 sequences (100000078 bp) in 1.984 CPU sec, 2.368 real sec...
[M::process] read 674894 sequences (100000154 bp) in 1.367 CPU sec, 1.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286190, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.35, 127.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 73, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (73, 73)
[M::mem_pestat] mean and std.dev: (73.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (73, 73)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675096 reads in 202.831 CPU sec, 6.583 real sec
[M::mem_process_seqs] Processed 674934 reads in 115.520 CPU sec, 3.718 real sec
sorting block 32, #reads = 1080336 mem = 536879514 peak = 1083731968
[M::process] read 674812 sequences (100000033 bp) in 1.866 CPU sec, 1.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285740, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (256.25, 127.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 1064, 1064)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3140)
[M::mem_pestat] mean and std.dev: (680.10, 642.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4178)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674796 sequences (100000278 bp) in 1.516 CPU sec, 1.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286053, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.88, 128.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286056, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.73, 130.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674850 reads in 298.081 CPU sec, 9.594 real sec
[M::mem_process_seqs] Processed 674894 reads in 207.020 CPU sec, 6.665 real sec
[M::mem_process_seqs] Processed 674812 reads in 216.017 CPU sec, 6.980 real sec
sorting block 33, #reads = 1080453 mem = 536882172 peak = 1083731968
[M::process] read 674786 sequences (100000101 bp) in 2.281 CPU sec, 2.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285903, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.16, 130.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1080354 mem = 536879624 peak = 1083731968
[M::process] read 674646 sequences (100000241 bp) in 2.140 CPU sec, 2.305 real sec...
[M::process] read 674796 sequences (100000003 bp) in 1.437 CPU sec, 1.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285623, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.11, 131.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 13, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2016)
[M::mem_pestat] mean and std.dev: (140.90, 266.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2687)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674796 reads in 210.891 CPU sec, 6.804 real sec
[M::mem_process_seqs] Processed 674786 reads in 119.202 CPU sec, 3.812 real sec
sorting block 35, #reads = 1080426 mem = 536880731 peak = 1083731968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285774, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674650 sequences (100000217 bp) in 2.288 CPU sec, 2.471 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.39, 132.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674798 sequences (100000066 bp) in 1.350 CPU sec, 1.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285828, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.60, 133.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674646 reads in 207.908 CPU sec, 6.697 real sec
[M::mem_process_seqs] Processed 674796 reads in 118.052 CPU sec, 3.795 real sec
sorting block 36, #reads = 1080437 mem = 536879279 peak = 1083731968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285292, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.06, 132.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674692 sequences (100000103 bp) in 2.082 CPU sec, 2.369 real sec...
[M::process] read 674506 sequences (100000029 bp) in 1.220 CPU sec, 1.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285689, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.06, 133.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674650 reads in 206.542 CPU sec, 6.678 real sec
[M::mem_process_seqs] Processed 674798 reads in 115.441 CPU sec, 3.715 real sec
sorting block 37, #reads = 1080088 mem = 536876657 peak = 1083731968
sorting block 38, #reads = 1080041 mem = 536878105 peak = 1094627328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285495, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674700 sequences (100000181 bp) in 2.272 CPU sec, 2.552 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.70, 133.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674692 sequences (100000191 bp) in 1.527 CPU sec, 1.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285648, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.79, 134.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674692 reads in 208.606 CPU sec, 6.724 real sec
[M::mem_process_seqs] Processed 674506 reads in 117.190 CPU sec, 3.772 real sec
sorting block 39, #reads = 1080445 mem = 536880661 peak = 1094627328
[M::process] read 674452 sequences (100000274 bp) in 1.933 CPU sec, 2.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285689, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.13, 134.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674324 sequences (100000097 bp) in 1.544 CPU sec, 1.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285838, 15, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.38, 135.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 15, 59)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 147)
[M::mem_pestat] mean and std.dev: (20.08, 21.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 191)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285399, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.12, 136.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674700 reads in 312.015 CPU sec, 10.047 real sec
[M::mem_process_seqs] Processed 674692 reads in 214.572 CPU sec, 6.915 real sec
[M::mem_process_seqs] Processed 674452 reads in 223.223 CPU sec, 7.213 real sec
sorting block 40, #reads = 1080166 mem = 536879139 peak = 1094627328
[M::process] read 674346 sequences (100000065 bp) in 2.226 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285426, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.95, 138.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1080768 mem = 536879573 peak = 1094627328
[M::process] read 674278 sequences (100000231 bp) in 2.195 CPU sec, 2.381 real sec...
[M::process] read 674510 sequences (100000104 bp) in 1.550 CPU sec, 1.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285233, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.90, 138.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 93, 1117)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3205)
[M::mem_pestat] mean and std.dev: (539.27, 527.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4249)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674324 reads in 215.989 CPU sec, 6.951 real sec
[M::mem_process_seqs] Processed 674346 reads in 119.880 CPU sec, 3.819 real sec
sorting block 42, #reads = 1080619 mem = 536879940 peak = 1094627328
[M::process] read 674278 sequences (100000051 bp) in 2.181 CPU sec, 2.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285092, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.30, 139.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674326 sequences (100000077 bp) in 1.626 CPU sec, 1.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284744, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.80, 138.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674278 reads in 217.341 CPU sec, 7.005 real sec
[M::mem_process_seqs] Processed 674510 reads in 121.426 CPU sec, 3.900 real sec
sorting block 43, #reads = 1080234 mem = 536881345 peak = 1094627328
[M::process] read 674306 sequences (100000014 bp) in 1.955 CPU sec, 2.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285177, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.69, 139.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 507, 2044)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6068)
[M::mem_pestat] mean and std.dev: (945.50, 1079.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8080)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674298 sequences (100000259 bp) in 1.515 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284847, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.09, 138.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285063, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.16, 138.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 7, 663)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985)
[M::mem_pestat] mean and std.dev: (213.20, 296.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2646)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674278 reads in 312.620 CPU sec, 10.062 real sec
[M::mem_process_seqs] Processed 674306 reads in 217.617 CPU sec, 7.013 real sec
[M::mem_process_seqs] Processed 674326 reads in 226.591 CPU sec, 7.331 real sec
sorting block 44, #reads = 1079807 mem = 536880548 peak = 1109004288
[M::process] read 674224 sequences (100000236 bp) in 2.222 CPU sec, 2.440 real sec...
sorting block 45, #reads = 1079250 mem = 536880201 peak = 1110102016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285031, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.20, 139.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674176 sequences (100000275 bp) in 1.999 CPU sec, 2.248 real sec...
[M::process] read 674166 sequences (100000038 bp) in 1.558 CPU sec, 1.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284845, 23, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.49, 142.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 15, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (136.23, 152.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674298 reads in 221.421 CPU sec, 7.181 real sec
[M::mem_process_seqs] Processed 674224 reads in 125.638 CPU sec, 4.042 real sec
sorting block 46, #reads = 1080140 mem = 536879274 peak = 1110102016
[M::process] read 674022 sequences (100000247 bp) in 2.109 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284591, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.09, 141.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674246 sequences (100000090 bp) in 1.558 CPU sec, 1.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284647, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.80, 141.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284640, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.96, 142.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674176 reads in 321.735 CPU sec, 10.368 real sec
sorting block 47, #reads = 1079789 mem = 536881513 peak = 1110102016
[M::mem_process_seqs] Processed 674166 reads in 222.267 CPU sec, 7.146 real sec
[M::mem_process_seqs] Processed 674022 reads in 229.647 CPU sec, 7.400 real sec
sorting block 48, #reads = 1079784 mem = 536878968 peak = 1110102016
[M::process] read 674630 sequences (100000148 bp) in 2.086 CPU sec, 2.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285272, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.91, 144.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 586, 586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1730)
[M::mem_pestat] mean and std.dev: (447.33, 500.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2451)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674752 sequences (100000005 bp) in 2.227 CPU sec, 2.514 real sec...
[M::process] read 674618 sequences (100000061 bp) in 1.488 CPU sec, 1.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285681, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.27, 137.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674246 reads in 221.737 CPU sec, 7.171 real sec
[M::mem_process_seqs] Processed 674630 reads in 124.266 CPU sec, 3.988 real sec
sorting block 49, #reads = 1079749 mem = 536878954 peak = 1110102016
sorting block 50, #reads = 1080651 mem = 536879715 peak = 1116372992
[M::process] read 674778 sequences (100000128 bp) in 2.247 CPU sec, 2.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286297, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.08, 134.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 88, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079)
[M::mem_pestat] mean and std.dev: (112.13, 138.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1428)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674678 sequences (100000055 bp) in 1.210 CPU sec, 1.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286197, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.54, 135.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674752 reads in 214.630 CPU sec, 6.917 real sec
[M::mem_process_seqs] Processed 674618 reads in 119.582 CPU sec, 3.856 real sec
sorting block 51, #reads = 1080756 mem = 536877981 peak = 1116372992
[M::process] read 674592 sequences (100000297 bp) in 2.173 CPU sec, 2.306 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285056, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.93, 134.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 441, 1606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4672)
[M::mem_pestat] mean and std.dev: (573.13, 646.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6205)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674576 sequences (100000288 bp) in 1.581 CPU sec, 1.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285156, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.68, 133.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 32, 2031)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6059)
[M::mem_pestat] mean and std.dev: (1094.91, 1800.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8297)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285540, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.29, 135.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (53, 381, 2312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6830)
[M::mem_pestat] mean and std.dev: (932.75, 995.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9089)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674778 reads in 319.985 CPU sec, 10.307 real sec
[M::mem_process_seqs] Processed 674678 reads in 221.462 CPU sec, 7.127 real sec
[M::mem_process_seqs] Processed 674592 reads in 230.550 CPU sec, 7.445 real sec
sorting block 52, #reads = 1080108 mem = 536877973 peak = 1116372992
[M::process] read 674584 sequences (100000207 bp) in 2.018 CPU sec, 2.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285513, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.62, 137.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1080157 mem = 536878360 peak = 1116372992
[M::process] read 674640 sequences (100000240 bp) in 2.201 CPU sec, 2.435 real sec...
[M::process] read 674656 sequences (100000163 bp) in 1.570 CPU sec, 1.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285392, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.96, 136.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 18, 128)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372)
[M::mem_pestat] mean and std.dev: (67.31, 86.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 494)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674576 reads in 222.156 CPU sec, 7.170 real sec
[M::mem_process_seqs] Processed 674584 reads in 122.503 CPU sec, 3.911 real sec
sorting block 54, #reads = 1080777 mem = 536882612 peak = 1116372992
[M::process] read 674474 sequences (100000246 bp) in 2.178 CPU sec, 2.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285730, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.88, 136.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 505, 602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1480)
[M::mem_pestat] mean and std.dev: (388.50, 262.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1919)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674564 sequences (100000197 bp) in 1.408 CPU sec, 1.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285892, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.85, 135.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674640 reads in 209.221 CPU sec, 6.776 real sec
[M::mem_process_seqs] Processed 674656 reads in 114.845 CPU sec, 3.744 real sec
sorting block 55, #reads = 1080590 mem = 536877156 peak = 1116372992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285213, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.38, 135.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674768 sequences (100000130 bp) in 2.209 CPU sec, 2.511 real sec...
[M::process] read 674492 sequences (100000290 bp) in 1.698 CPU sec, 1.705 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285642, 22, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.26, 138.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 448, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1292)
[M::mem_pestat] mean and std.dev: (294.68, 231.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1714)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674474 reads in 211.793 CPU sec, 6.826 real sec
[M::mem_process_seqs] Processed 674564 reads in 118.104 CPU sec, 3.780 real sec
sorting block 56, #reads = 1079988 mem = 536877606 peak = 1116372992
sorting block 57, #reads = 1080236 mem = 536879271 peak = 1116372992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286416, 18, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674346 sequences (100000048 bp) in 2.231 CPU sec, 2.411 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.60, 139.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (55, 55, 146)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328)
[M::mem_pestat] mean and std.dev: (83.06, 75.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 419)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674470 sequences (100000019 bp) in 1.600 CPU sec, 1.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285016, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (268.63, 139.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674768 reads in 205.441 CPU sec, 6.592 real sec
[M::mem_process_seqs] Processed 674492 reads in 116.092 CPU sec, 3.735 real sec
sorting block 58, #reads = 1080427 mem = 536878853 peak = 1116372992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285837, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.13, 138.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (418, 558, 679)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201)
[M::mem_pestat] mean and std.dev: (434.64, 270.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1516)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674844 sequences (100000146 bp) in 2.163 CPU sec, 2.464 real sec...
[M::process] read 675074 sequences (100000266 bp) in 1.566 CPU sec, 1.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285385, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.69, 139.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674346 reads in 208.519 CPU sec, 6.725 real sec
[M::mem_process_seqs] Processed 674470 reads in 115.852 CPU sec, 3.718 real sec
sorting block 59, #reads = 1080020 mem = 536880874 peak = 1116372992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285864, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.20, 129.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 158, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171)
[M::mem_pestat] mean and std.dev: (221.73, 203.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1553)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674960 sequences (100000170 bp) in 2.240 CPU sec, 2.451 real sec...
[M::process] read 675038 sequences (100000100 bp) in 1.607 CPU sec, 1.610 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286512, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.96, 124.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 88, 88)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 132)
[M::mem_pestat] mean and std.dev: (85.45, 10.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 154)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674844 reads in 208.966 CPU sec, 6.740 real sec
[M::mem_process_seqs] Processed 675074 reads in 117.559 CPU sec, 3.791 real sec
sorting block 60, #reads = 1079809 mem = 536877990 peak = 1116372992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285566, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (255.29, 126.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674972 sequences (100000194 bp) in 2.185 CPU sec, 2.291 real sec...
[M::process] read 674964 sequences (100000234 bp) in 1.629 CPU sec, 1.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286376, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (253.19, 124.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674960 reads in 195.577 CPU sec, 6.259 real sec
[M::mem_process_seqs] Processed 675038 reads in 108.072 CPU sec, 3.445 real sec
sorting block 61, #reads = 1080881 mem = 536877521 peak = 1116372992
sorting block 62, #reads = 1080051 mem = 536878196 peak = 1116372992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285730, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.79, 125.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674868 sequences (100000244 bp) in 2.144 CPU sec, 2.266 real sec...
[M::process] read 674836 sequences (100000069 bp) in 1.636 CPU sec, 1.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286210, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.72, 126.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 67, 132)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344)
[M::mem_pestat] mean and std.dev: (49.27, 38.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 450)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674972 reads in 199.607 CPU sec, 6.382 real sec
[M::mem_process_seqs] Processed 674964 reads in 115.125 CPU sec, 3.681 real sec
sorting block 63, #reads = 1080153 mem = 536879199 peak = 1116372992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285618, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.99, 128.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674686 sequences (100000220 bp) in 2.289 CPU sec, 2.408 real sec...
[M::process] read 674734 sequences (100000065 bp) in 1.673 CPU sec, 1.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285914, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.36, 128.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674868 reads in 207.907 CPU sec, 6.661 real sec
[M::mem_process_seqs] Processed 674836 reads in 116.516 CPU sec, 3.718 real sec
sorting block 64, #reads = 1080434 mem = 536878619 peak = 1116372992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285674, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (259.10, 130.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 280, 1414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4152)
[M::mem_pestat] mean and std.dev: (480.83, 547.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5521)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674742 sequences (100000008 bp) in 2.387 CPU sec, 2.529 real sec...
[M::process] read 674562 sequences (100000085 bp) in 1.678 CPU sec, 1.679 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285781, 19, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.59, 131.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 315, 3852)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11490)
[M::mem_pestat] mean and std.dev: (1503.32, 1797.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15309)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674686 reads in 206.098 CPU sec, 6.602 real sec
[M::mem_process_seqs] Processed 674734 reads in 115.394 CPU sec, 3.674 real sec
sorting block 65, #reads = 1079835 mem = 536879291 peak = 1116372992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285630, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.41, 132.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674666 sequences (100000174 bp) in 2.474 CPU sec, 2.642 real sec...
[M::process] read 674680 sequences (100000206 bp) in 1.740 CPU sec, 1.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285648, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.38, 132.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 81)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 237)
[M::mem_pestat] mean and std.dev: (28.85, 37.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 315)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674742 reads in 207.815 CPU sec, 6.664 real sec
[M::mem_process_seqs] Processed 674562 reads in 117.561 CPU sec, 3.750 real sec
sorting block 66, #reads = 1080130 mem = 536878709 peak = 1116372992
sorting block 67, #reads = 1080388 mem = 536878473 peak = 1116372992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285200, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.09, 133.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 22, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1487)
[M::mem_pestat] mean and std.dev: (155.69, 199.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1977)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674732 sequences (100000264 bp) in 2.472 CPU sec, 2.618 real sec...
[M::process] read 674796 sequences (100000093 bp) in 1.668 CPU sec, 1.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285561, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.51, 133.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674666 reads in 203.089 CPU sec, 6.495 real sec
[M::mem_process_seqs] Processed 674680 reads in 109.508 CPU sec, 3.481 real sec
sorting block 68, #reads = 1080175 mem = 536877914 peak = 1116372992
[M::process] read 674558 sequences (100000007 bp) in 2.369 CPU sec, 2.521 real sec...
[M::process] read 674572 sequences (100000036 bp) in 1.679 CPU sec, 1.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285708, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.54, 134.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674796 reads in 107.302 CPU sec, 3.408 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285606, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.61, 133.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674550 sequences (100000043 bp) in 1.512 CPU sec, 1.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284739, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.41, 136.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674732 reads in 302.841 CPU sec, 9.662 real sec
sorting block 69, #reads = 1080069 mem = 536877594 peak = 1116372992
[M::mem_process_seqs] Processed 674558 reads in 121.293 CPU sec, 3.892 real sec
sorting block 70, #reads = 1080409 mem = 536878910 peak = 1116372992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285629, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (267.12, 138.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674354 sequences (100000200 bp) in 2.320 CPU sec, 2.487 real sec...
[M::process] read 674354 sequences (100000104 bp) in 1.398 CPU sec, 1.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285225, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.56, 138.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 7, 18)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 40)
[M::mem_pestat] mean and std.dev: (6.93, 4.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 51)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674572 reads in 201.391 CPU sec, 6.445 real sec
[M::mem_process_seqs] Processed 674550 reads in 111.764 CPU sec, 3.574 real sec
sorting block 71, #reads = 1079504 mem = 536877461 peak = 1116372992
[M::process] read 674452 sequences (100000225 bp) in 1.912 CPU sec, 2.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285459, 14, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.41, 137.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (480, 1830, 2753)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7299)
[M::mem_pestat] mean and std.dev: (1729.29, 1775.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9572)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674382 sequences (100000214 bp) in 1.347 CPU sec, 1.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284368, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.52, 137.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285158, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.45, 139.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 11, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (38.40, 66.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674354 reads in 295.971 CPU sec, 9.513 real sec
[M::mem_process_seqs] Processed 674354 reads in 205.406 CPU sec, 6.618 real sec
[M::mem_process_seqs] Processed 674452 reads in 213.197 CPU sec, 6.887 real sec
sorting block 72, #reads = 1080238 mem = 536879673 peak = 1116372992
sorting block 73, #reads = 1080155 mem = 536879761 peak = 1116372992
[M::process] read 674250 sequences (100000285 bp) in 2.516 CPU sec, 2.739 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284904, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.23, 138.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (104, 474, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214)
[M::mem_pestat] mean and std.dev: (417.36, 313.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1673)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674244 sequences (100000194 bp) in 1.947 CPU sec, 2.022 real sec...
[M::process] read 674256 sequences (100000122 bp) in 1.510 CPU sec, 1.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284939, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.96, 139.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 22, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233)
[M::mem_pestat] mean and std.dev: (20.80, 23.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 306)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674382 reads in 208.813 CPU sec, 6.649 real sec
[M::mem_process_seqs] Processed 674250 reads in 116.365 CPU sec, 3.688 real sec
sorting block 74, #reads = 1080147 mem = 536877695 peak = 1116372992
[M::process] read 674500 sequences (100000022 bp) in 2.162 CPU sec, 2.290 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284545, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.09, 138.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674214 sequences (100000178 bp) in 1.530 CPU sec, 1.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284588, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.34, 139.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285115, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.31, 140.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674244 reads in 300.183 CPU sec, 9.615 real sec
[M::mem_process_seqs] Processed 674500 reads in 207.294 CPU sec, 6.667 real sec
[M::mem_process_seqs] Processed 674256 reads in 214.871 CPU sec, 6.928 real sec
sorting block 75, #reads = 1080075 mem = 536881073 peak = 1116372992
sorting block 76, #reads = 1079754 mem = 536879672 peak = 1116372992
[M::process] read 674280 sequences (100000041 bp) in 2.432 CPU sec, 2.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284575, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.05, 139.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674184 sequences (100000107 bp) in 1.553 CPU sec, 1.627 real sec...
[M::process] read 674194 sequences (100000036 bp) in 1.523 CPU sec, 1.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284700, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.41, 140.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674214 reads in 207.002 CPU sec, 6.611 real sec
[M::mem_process_seqs] Processed 674280 reads in 115.479 CPU sec, 3.666 real sec
sorting block 77, #reads = 1079693 mem = 536877839 peak = 1116372992
[M::process] read 674380 sequences (100000152 bp) in 2.120 CPU sec, 2.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283999, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.84, 140.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674732 sequences (100000245 bp) in 1.520 CPU sec, 1.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284543, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.21, 140.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 202, 2743)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7825)
[M::mem_pestat] mean and std.dev: (799.92, 1069.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10366)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284731, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (268.24, 138.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674184 reads in 294.076 CPU sec, 9.417 real sec
[M::mem_process_seqs] Processed 674194 reads in 202.087 CPU sec, 6.499 real sec
sorting block 78, #reads = 1079685 mem = 536879378 peak = 1116372992
[M::mem_process_seqs] Processed 674380 reads in 210.408 CPU sec, 6.788 real sec
sorting block 79, #reads = 1079106 mem = 536879573 peak = 1160482816
[M::process] read 674772 sequences (100000236 bp) in 2.300 CPU sec, 2.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285027, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.48, 135.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 145, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1672)
[M::mem_pestat] mean and std.dev: (287.45, 273.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2210)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674662 sequences (100000053 bp) in 1.785 CPU sec, 1.868 real sec...
[M::process] read 674592 sequences (100000107 bp) in 1.550 CPU sec, 1.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285796, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.28, 133.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674732 reads in 200.826 CPU sec, 6.385 real sec
[M::mem_process_seqs] Processed 674772 reads in 111.351 CPU sec, 3.519 real sec
sorting block 80, #reads = 1080120 mem = 536877647 peak = 1160482816
[M::process] read 674598 sequences (100000110 bp) in 2.194 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285219, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.98, 133.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674592 sequences (100000129 bp) in 1.600 CPU sec, 1.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285461, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.45, 134.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285332, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.40, 134.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 380, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2058)
[M::mem_pestat] mean and std.dev: (388.45, 371.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2721)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674662 reads in 290.852 CPU sec, 9.309 real sec
sorting block 81, #reads = 1080139 mem = 536879530 peak = 1160482816
[M::mem_process_seqs] Processed 674592 reads in 200.761 CPU sec, 6.447 real sec
[M::mem_process_seqs] Processed 674598 reads in 209.041 CPU sec, 6.733 real sec
sorting block 82, #reads = 1080080 mem = 536878244 peak = 1160482816
[M::process] read 674608 sequences (100000108 bp) in 2.347 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284747, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.78, 134.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674552 sequences (100000160 bp) in 1.767 CPU sec, 1.834 real sec...
[M::process] read 674448 sequences (100000279 bp) in 1.530 CPU sec, 1.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285291, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.03, 135.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 197, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (169.54, 164.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674592 reads in 201.375 CPU sec, 6.410 real sec
[M::mem_process_seqs] Processed 674608 reads in 111.246 CPU sec, 3.514 real sec
sorting block 83, #reads = 1080221 mem = 536880263 peak = 1160482816
sorting block 84, #reads = 1080092 mem = 536880494 peak = 1174888448
[M::process] read 674708 sequences (100000298 bp) in 2.037 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285085, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.79, 135.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674648 sequences (100000264 bp) in 1.530 CPU sec, 1.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284778, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.90, 136.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 58, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (166.46, 196.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1381)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285233, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.57, 134.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674552 reads in 282.175 CPU sec, 9.033 real sec
[M::mem_process_seqs] Processed 674448 reads in 193.221 CPU sec, 6.212 real sec
[M::mem_process_seqs] Processed 674708 reads in 201.665 CPU sec, 6.502 real sec
sorting block 85, #reads = 1080457 mem = 536876793 peak = 1174888448
[M::process] read 674566 sequences (100000043 bp) in 2.298 CPU sec, 2.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285027, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.35, 136.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 86, #reads = 1079591 mem = 536878475 peak = 1174888448
[M::process] read 674518 sequences (100000203 bp) in 1.814 CPU sec, 1.877 real sec...
[M::process] read 674444 sequences (100000091 bp) in 1.537 CPU sec, 1.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285615, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.16, 138.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 8, 8)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 8)
[M::mem_pestat] mean and std.dev: (8.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (8, 8)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674648 reads in 200.508 CPU sec, 6.380 real sec
[M::mem_process_seqs] Processed 674566 reads in 110.538 CPU sec, 3.492 real sec
sorting block 87, #reads = 1080348 mem = 536879178 peak = 1174888448
[M::process] read 674396 sequences (100000072 bp) in 2.105 CPU sec, 2.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285347, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.37, 137.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674340 sequences (100000142 bp) in 1.500 CPU sec, 1.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285452, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (268.44, 139.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (104, 350, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (253.71, 185.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285508, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.71, 139.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 13, 39)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 111)
[M::mem_pestat] mean and std.dev: (18.50, 17.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 147)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674518 reads in 283.802 CPU sec, 9.092 real sec
[M::mem_process_seqs] Processed 674444 reads in 194.189 CPU sec, 6.246 real sec
[M::mem_process_seqs] Processed 674396 reads in 202.616 CPU sec, 6.536 real sec
sorting block 88, #reads = 1080106 mem = 536881313 peak = 1174888448
[M::process] read 674796 sequences (100000070 bp) in 2.059 CPU sec, 2.217 real sec...
sorting block 89, #reads = 1079256 mem = 536878805 peak = 1174888448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285192, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.84, 137.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675238 sequences (100000155 bp) in 1.513 CPU sec, 1.587 real sec...
[M::process] read 675066 sequences (100000297 bp) in 1.140 CPU sec, 1.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285943, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.04, 129.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674340 reads in 191.875 CPU sec, 6.124 real sec
[M::mem_process_seqs] Processed 674796 reads in 105.637 CPU sec, 3.367 real sec
sorting block 90, #reads = 1079276 mem = 536880291 peak = 1174888448
[M::process] read 674886 sequences (100000033 bp) in 1.996 CPU sec, 2.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286258, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.88, 123.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 59, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (163.58, 196.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1391)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675028 sequences (100000020 bp) in 1.500 CPU sec, 1.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285382, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (252.25, 124.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285602, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (253.33, 124.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675238 reads in 276.837 CPU sec, 8.864 real sec
[M::mem_process_seqs] Processed 675066 reads in 193.197 CPU sec, 6.204 real sec
[M::mem_process_seqs] Processed 674886 reads in 201.678 CPU sec, 6.498 real sec
sorting block 91, #reads = 1079617 mem = 536879496 peak = 1174888448
sorting block 92, #reads = 1080153 mem = 536880056 peak = 1174888448
[M::process] read 674938 sequences (100000243 bp) in 2.436 CPU sec, 2.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285664, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (253.64, 125.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674878 sequences (100000087 bp) in 1.526 CPU sec, 1.565 real sec...
[M::process] read 674830 sequences (100000032 bp) in 1.450 CPU sec, 1.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285811, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (254.46, 125.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675028 reads in 192.571 CPU sec, 6.133 real sec
[M::mem_process_seqs] Processed 674938 reads in 106.005 CPU sec, 3.354 real sec
sorting block 93, #reads = 1080112 mem = 536879400 peak = 1174888448
[M::process] read 674718 sequences (100000281 bp) in 2.154 CPU sec, 2.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285987, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (254.49, 125.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 218, 1427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4231)
[M::mem_pestat] mean and std.dev: (488.40, 621.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5633)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674778 sequences (100000190 bp) in 1.520 CPU sec, 1.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285410, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (256.64, 127.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 13, 2753)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8251)
[M::mem_pestat] mean and std.dev: (883.46, 1248.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11000)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285230, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (256.60, 127.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674878 reads in 280.672 CPU sec, 8.985 real sec
[M::mem_process_seqs] Processed 674830 reads in 196.282 CPU sec, 6.305 real sec
[M::mem_process_seqs] Processed 674718 reads in 204.290 CPU sec, 6.580 real sec
sorting block 94, #reads = 1080339 mem = 536879438 peak = 1174888448
sorting block 95, #reads = 1080262 mem = 536878994 peak = 1174888448
[M::process] read 674684 sequences (100000221 bp) in 2.362 CPU sec, 2.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284665, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (256.27, 127.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 34, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242)
[M::mem_pestat] mean and std.dev: (35.85, 31.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 318)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674704 sequences (100000238 bp) in 1.617 CPU sec, 1.693 real sec...
[M::process] read 674674 sequences (100000200 bp) in 1.485 CPU sec, 1.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285350, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.76, 129.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 22, 824)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2428)
[M::mem_pestat] mean and std.dev: (401.92, 729.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3318)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674778 reads in 194.455 CPU sec, 6.182 real sec
[M::mem_process_seqs] Processed 674684 reads in 107.912 CPU sec, 3.409 real sec
sorting block 96, #reads = 1080522 mem = 536880344 peak = 1174888448
[M::process] read 674676 sequences (100000056 bp) in 2.111 CPU sec, 2.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284698, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.32, 130.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 360, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058)
[M::mem_pestat] mean and std.dev: (232.45, 179.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1407)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674622 sequences (100000134 bp) in 1.430 CPU sec, 1.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284398, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.17, 131.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285349, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.15, 132.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674704 reads in 285.519 CPU sec, 9.145 real sec
[M::mem_process_seqs] Processed 674674 reads in 199.613 CPU sec, 6.414 real sec
sorting block 97, #reads = 1079919 mem = 536878140 peak = 1174888448
[M::mem_process_seqs] Processed 674676 reads in 207.581 CPU sec, 6.688 real sec
sorting block 98, #reads = 1079415 mem = 536879383 peak = 1174888448
[M::process] read 674616 sequences (100000147 bp) in 2.162 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285250, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.70, 132.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674550 sequences (100000110 bp) in 1.472 CPU sec, 1.523 real sec...
[M::process] read 674440 sequences (100000159 bp) in 1.170 CPU sec, 1.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285675, 22, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.67, 133.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 974, 2788)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8080)
[M::mem_pestat] mean and std.dev: (1168.05, 1193.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10726)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674622 reads in 198.371 CPU sec, 6.328 real sec
[M::mem_process_seqs] Processed 674616 reads in 108.159 CPU sec, 3.435 real sec
sorting block 99, #reads = 1079952 mem = 536881919 peak = 1174888448
[M::process] read 674452 sequences (100000156 bp) in 2.173 CPU sec, 2.281 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284491, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (264.09, 134.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 920, 2879)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8611)
[M::mem_pestat] mean and std.dev: (1095.62, 1373.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11477)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674378 sequences (100000229 bp) in 1.479 CPU sec, 1.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285292, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.72, 134.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285368, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.64, 136.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 55, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1681)
[M::mem_pestat] mean and std.dev: (261.40, 303.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2239)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674550 reads in 284.969 CPU sec, 9.124 real sec
[M::mem_process_seqs] Processed 674440 reads in 196.470 CPU sec, 6.315 real sec
sorting block 100, #reads = 1080207 mem = 536877703 peak = 1174888448
[M::mem_process_seqs] Processed 674452 reads in 204.518 CPU sec, 6.593 real sec
sorting block 101, #reads = 1079880 mem = 536878262 peak = 1174888448
[M::process] read 674386 sequences (100000268 bp) in 2.287 CPU sec, 2.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285042, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.66, 136.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674514 sequences (100000286 bp) in 1.414 CPU sec, 1.475 real sec...
[M::process] read 674218 sequences (100000126 bp) in 1.170 CPU sec, 1.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284467, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.87, 137.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674378 reads in 201.106 CPU sec, 6.420 real sec
[M::mem_process_seqs] Processed 674386 reads in 112.708 CPU sec, 3.591 real sec
sorting block 102, #reads = 1080334 mem = 536881063 peak = 1174888448
sorting block 103, #reads = 1079721 mem = 536877574 peak = 1174888448
[M::process] read 674322 sequences (100000007 bp) in 1.648 CPU sec, 1.748 real sec...
[M::process] read 674308 sequences (100000064 bp) in 1.100 CPU sec, 1.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284425, 20, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.83, 136.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (377, 542, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 872)
[M::mem_pestat] mean and std.dev: (446.26, 184.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674218 reads in 104.950 CPU sec, 3.386 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284828, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (266.59, 136.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 91, 180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440)
[M::mem_pestat] mean and std.dev: (106.00, 88.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 570)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674364 sequences (100000157 bp) in 1.469 CPU sec, 1.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284538, 26, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.51, 136.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 409, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163)
[M::mem_pestat] mean and std.dev: (301.33, 233.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1540)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674514 reads in 300.624 CPU sec, 9.609 real sec
[M::mem_process_seqs] Processed 674322 reads in 210.399 CPU sec, 6.756 real sec
sorting block 104, #reads = 1079864 mem = 536876921 peak = 1174888448
[M::process] read 674256 sequences (100000168 bp) in 2.177 CPU sec, 2.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284036, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.66, 136.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 61, 1178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3412)
[M::mem_pestat] mean and std.dev: (487.75, 526.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4529)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674336 sequences (100000223 bp) in 1.490 CPU sec, 1.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284402, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.56, 135.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284533, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (267.33, 137.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674308 reads in 300.019 CPU sec, 9.614 real sec
sorting block 105, #reads = 1079691 mem = 536879245 peak = 1174888448
[M::mem_process_seqs] Processed 674364 reads in 210.088 CPU sec, 6.739 real sec
[M::mem_process_seqs] Processed 674256 reads in 217.995 CPU sec, 7.012 real sec
sorting block 106, #reads = 1079513 mem = 536880162 peak = 1189421056
[M::process] read 674204 sequences (100000013 bp) in 2.329 CPU sec, 2.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284015, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.84, 138.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674392 sequences (100000019 bp) in 1.836 CPU sec, 1.911 real sec...
[M::process] read 674336 sequences (100000181 bp) in 1.448 CPU sec, 1.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284699, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.96, 138.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674336 reads in 209.813 CPU sec, 6.680 real sec
[M::mem_process_seqs] Processed 674204 reads in 115.888 CPU sec, 3.672 real sec
sorting block 107, #reads = 1079790 mem = 536878775 peak = 1189421056
sorting block 108, #reads = 1079783 mem = 536878696 peak = 1189421056
[M::process] read 674422 sequences (100000071 bp) in 1.986 CPU sec, 2.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284034, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.12, 137.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674672 sequences (100000176 bp) in 1.479 CPU sec, 1.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283989, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.77, 136.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285333, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (265.61, 135.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674392 reads in 297.659 CPU sec, 9.540 real sec
[M::mem_process_seqs] Processed 674336 reads in 206.076 CPU sec, 6.625 real sec
[M::mem_process_seqs] Processed 674422 reads in 214.133 CPU sec, 6.909 real sec
sorting block 109, #reads = 1080173 mem = 536879328 peak = 1189421056
[M::process] read 674670 sequences (100000132 bp) in 2.308 CPU sec, 2.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285406, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.81, 131.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674674 sequences (100000211 bp) in 1.620 CPU sec, 1.704 real sec...
[M::process] read 674656 sequences (100000156 bp) in 1.495 CPU sec, 1.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285501, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.61, 131.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 66, 1849)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5543)
[M::mem_pestat] mean and std.dev: (629.92, 821.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7390)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674672 reads in 200.448 CPU sec, 6.374 real sec
sorting block 110, #reads = 1080181 mem = 536879478 peak = 1189421056
[M::mem_process_seqs] Processed 674670 reads in 109.590 CPU sec, 3.469 real sec
sorting block 111, #reads = 1080516 mem = 536877457 peak = 1189421056
[M::process] read 674662 sequences (100000110 bp) in 2.038 CPU sec, 2.162 real sec...
[M::process] read 674710 sequences (100000243 bp) in 1.550 CPU sec, 1.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285246, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.89, 133.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 88, 128)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358)
[M::mem_pestat] mean and std.dev: (57.56, 49.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 473)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674662 reads in 105.125 CPU sec, 3.362 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284916, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.34, 131.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674504 sequences (100000035 bp) in 1.528 CPU sec, 1.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285291, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.90, 132.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 178, 191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (152, 217)
[M::mem_pestat] mean and std.dev: (179.86, 4.55)
[M::mem_pestat] low and high boundaries for proper pairs: (139, 230)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674674 reads in 299.061 CPU sec, 9.544 real sec
[M::mem_process_seqs] Processed 674656 reads in 210.262 CPU sec, 6.718 real sec
sorting block 112, #reads = 1080076 mem = 536878813 peak = 1189421056
sorting block 113, #reads = 1080120 mem = 536877713 peak = 1208496128
[M::process] read 674576 sequences (100000108 bp) in 1.842 CPU sec, 1.912 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285401, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.23, 132.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674504 sequences (100000185 bp) in 1.422 CPU sec, 1.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285133, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.46, 130.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285280, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.79, 132.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 357, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280)
[M::mem_pestat] mean and std.dev: (300.31, 207.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1645)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674710 reads in 275.230 CPU sec, 8.813 real sec
[M::mem_process_seqs] Processed 674504 reads in 189.807 CPU sec, 6.105 real sec
[M::mem_process_seqs] Processed 674576 reads in 197.973 CPU sec, 6.390 real sec
sorting block 114, #reads = 1080394 mem = 536880132 peak = 1208496128
[M::process] read 674442 sequences (100000290 bp) in 2.439 CPU sec, 2.641 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284870, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.95, 134.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 115, #reads = 1080336 mem = 536879074 peak = 1208496128
[M::process] read 674662 sequences (100000088 bp) in 1.769 CPU sec, 1.837 real sec...
[M::process] read 674540 sequences (100000115 bp) in 1.109 CPU sec, 1.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285379, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.55, 134.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 104, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (95.42, 83.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674504 reads in 200.059 CPU sec, 6.390 real sec
[M::mem_process_seqs] Processed 674442 reads in 110.937 CPU sec, 3.521 real sec
sorting block 116, #reads = 1079663 mem = 536878598 peak = 1208496128
[M::process] read 674434 sequences (100000118 bp) in 2.116 CPU sec, 2.243 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284944, 24, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.63, 133.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 370, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2144)
[M::mem_pestat] mean and std.dev: (226.95, 253.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2854)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674366 sequences (100000273 bp) in 1.550 CPU sec, 1.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284874, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.90, 134.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285142, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.65, 137.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674662 reads in 279.432 CPU sec, 8.946 real sec
[M::mem_process_seqs] Processed 674540 reads in 194.880 CPU sec, 6.255 real sec
[M::mem_process_seqs] Processed 674434 reads in 203.134 CPU sec, 6.541 real sec
sorting block 117, #reads = 1080053 mem = 536880053 peak = 1208496128
[M::process] read 674290 sequences (100000210 bp) in 1.877 CPU sec, 2.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285333, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.96, 137.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 13, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (120.60, 182.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 118, #reads = 1079812 mem = 536881777 peak = 1208496128
[M::process] read 674296 sequences (100000160 bp) in 2.114 CPU sec, 2.434 real sec...
[M::process] read 674568 sequences (100000051 bp) in 1.489 CPU sec, 1.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285646, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.36, 138.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 20, 122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346)
[M::mem_pestat] mean and std.dev: (35.86, 41.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 458)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674366 reads in 205.521 CPU sec, 6.626 real sec
[M::mem_process_seqs] Processed 674290 reads in 114.734 CPU sec, 3.642 real sec
sorting block 119, #reads = 1080084 mem = 536880891 peak = 1208496128
[M::process] read 675342 sequences (100000273 bp) in 2.101 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285791, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.34, 138.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 25, 37)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 107)
[M::mem_pestat] mean and std.dev: (14.75, 12.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 142)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675234 sequences (100000171 bp) in 1.510 CPU sec, 1.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285254, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.03, 135.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 247, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1533)
[M::mem_pestat] mean and std.dev: (320.10, 394.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2041)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285056, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.74, 127.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674296 reads in 305.660 CPU sec, 9.814 real sec
[M::mem_process_seqs] Processed 674568 reads in 219.029 CPU sec, 7.024 real sec
[M::mem_process_seqs] Processed 675342 reads in 227.478 CPU sec, 7.318 real sec
sorting block 120, #reads = 1079704 mem = 536879317 peak = 1208496128
[M::process] read 675232 sequences (100000152 bp) in 2.258 CPU sec, 2.489 real sec...
sorting block 121, #reads = 1079924 mem = 536876623 peak = 1208496128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285195, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.11, 128.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 626, 9586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28680)
[M::mem_pestat] mean and std.dev: (3081.30, 4263.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38227)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675112 sequences (100000154 bp) in 1.953 CPU sec, 2.067 real sec...
[M::process] read 674888 sequences (100000000 bp) in 1.396 CPU sec, 1.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285484, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.56, 130.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 53, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (192.00, 221.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675234 reads in 216.572 CPU sec, 6.936 real sec
[M::mem_process_seqs] Processed 675232 reads in 118.039 CPU sec, 3.755 real sec
sorting block 122, #reads = 1080308 mem = 536878429 peak = 1208496128
[M::process] read 674990 sequences (100000081 bp) in 2.206 CPU sec, 2.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284600, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.43, 130.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 32, 1109)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3315)
[M::mem_pestat] mean and std.dev: (408.23, 490.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4418)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674942 sequences (100000196 bp) in 1.532 CPU sec, 1.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284429, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.80, 131.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284665, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.82, 131.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675112 reads in 321.357 CPU sec, 10.317 real sec
[M::mem_process_seqs] Processed 674888 reads in 222.972 CPU sec, 7.159 real sec
[M::mem_process_seqs] Processed 674990 reads in 231.227 CPU sec, 7.451 real sec
sorting block 123, #reads = 1080065 mem = 536879247 peak = 1208496128
sorting block 124, #reads = 1079524 mem = 536879038 peak = 1216606208
[M::process] read 674944 sequences (100000032 bp) in 2.299 CPU sec, 2.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285028, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.54, 131.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 25, 42)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 120)
[M::mem_pestat] mean and std.dev: (23.62, 22.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 159)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674790 sequences (100000236 bp) in 1.882 CPU sec, 1.979 real sec...
[M::process] read 674910 sequences (100000270 bp) in 1.522 CPU sec, 1.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284656, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.09, 131.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674942 reads in 221.483 CPU sec, 7.088 real sec
[M::mem_process_seqs] Processed 674944 reads in 123.000 CPU sec, 3.916 real sec
sorting block 125, #reads = 1079958 mem = 536880991 peak = 1216606208
[M::process] read 674960 sequences (100000118 bp) in 2.075 CPU sec, 2.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284747, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.20, 131.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674792 sequences (100000079 bp) in 1.500 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284900, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.71, 131.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 533, 1270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3784)
[M::mem_pestat] mean and std.dev: (483.62, 553.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5041)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284836, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.59, 131.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674790 reads in 321.262 CPU sec, 10.319 real sec
[M::mem_process_seqs] Processed 674910 reads in 224.016 CPU sec, 7.191 real sec
[M::mem_process_seqs] Processed 674960 reads in 232.422 CPU sec, 7.483 real sec
sorting block 126, #reads = 1079960 mem = 536876743 peak = 1216606208
sorting block 127, #reads = 1080049 mem = 536877007 peak = 1217835008
[M::process] read 674906 sequences (100000164 bp) in 2.188 CPU sec, 2.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284722, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.86, 129.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674914 sequences (100000165 bp) in 1.907 CPU sec, 2.021 real sec...
[M::process] read 674776 sequences (100000171 bp) in 1.615 CPU sec, 1.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285559, 22, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (258.52, 132.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 488, 577)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1585)
[M::mem_pestat] mean and std.dev: (369.00, 287.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2089)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674792 reads in 224.302 CPU sec, 7.170 real sec
[M::mem_process_seqs] Processed 674906 reads in 122.523 CPU sec, 3.885 real sec
sorting block 128, #reads = 1080070 mem = 536879865 peak = 1217835008
[M::process] read 674692 sequences (100000021 bp) in 1.875 CPU sec, 2.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285375, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.06, 131.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 179, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1399)
[M::mem_pestat] mean and std.dev: (228.23, 194.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1845)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674666 sequences (100000034 bp) in 1.568 CPU sec, 1.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285085, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (259.60, 133.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 87, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1596)
[M::mem_pestat] mean and std.dev: (220.00, 235.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2105)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284678, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.56, 132.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674914 reads in 323.376 CPU sec, 10.395 real sec
[M::mem_process_seqs] Processed 674776 reads in 225.123 CPU sec, 7.239 real sec
sorting block 129, #reads = 1080708 mem = 536878960 peak = 1218572288
[M::mem_process_seqs] Processed 674692 reads in 234.417 CPU sec, 7.558 real sec
sorting block 130, #reads = 1079943 mem = 536878927 peak = 1220878336
[M::process] read 674534 sequences (100000255 bp) in 2.041 CPU sec, 2.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284247, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.77, 132.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 283, 790)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1940)
[M::mem_pestat] mean and std.dev: (442.36, 308.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2515)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674674 sequences (100000180 bp) in 1.952 CPU sec, 2.102 real sec...
[M::process] read 674450 sequences (100000228 bp) in 1.601 CPU sec, 1.608 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284389, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.15, 137.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 18, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 147)
[M::mem_pestat] mean and std.dev: (30.67, 24.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 190)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674666 reads in 222.173 CPU sec, 7.125 real sec
[M::mem_process_seqs] Processed 674534 reads in 126.069 CPU sec, 3.997 real sec
sorting block 131, #reads = 1077869 mem = 536878045 peak = 1220878336
sorting block 132, #reads = 1079081 mem = 536878874 peak = 1223946240
[M::process] read 674466 sequences (100000014 bp) in 2.130 CPU sec, 2.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284696, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.23, 137.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 172, 1135)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3341)
[M::mem_pestat] mean and std.dev: (507.25, 553.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4444)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674556 sequences (100000057 bp) in 1.610 CPU sec, 1.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284255, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.09, 137.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 66, 66)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 164)
[M::mem_pestat] mean and std.dev: (46.40, 24.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 213)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284386, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.27, 138.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674674 reads in 350.236 CPU sec, 11.259 real sec
[M::mem_process_seqs] Processed 674466 reads in 243.596 CPU sec, 7.840 real sec
[M::mem_process_seqs] Processed 674450 reads in 252.930 CPU sec, 8.157 real sec
sorting block 133, #reads = 1079236 mem = 536879892 peak = 1223946240
[M::process] read 674478 sequences (100000177 bp) in 2.190 CPU sec, 2.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283839, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.75, 137.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674412 sequences (100000168 bp) in 1.971 CPU sec, 2.041 real sec...
[M::process] read 674478 sequences (100000278 bp) in 1.618 CPU sec, 1.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284611, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.29, 139.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674556 reads in 237.531 CPU sec, 7.638 real sec
sorting block 134, #reads = 1079711 mem = 536879702 peak = 1223946240
[M::mem_process_seqs] Processed 674478 reads in 133.065 CPU sec, 4.246 real sec
sorting block 135, #reads = 1078772 mem = 536880830 peak = 1223946240
[M::process] read 674584 sequences (100000020 bp) in 2.132 CPU sec, 2.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284696, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.24, 139.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674592 sequences (100000125 bp) in 1.583 CPU sec, 1.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284559, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.67, 140.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284548, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.53, 138.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674412 reads in 336.427 CPU sec, 10.829 real sec
[M::mem_process_seqs] Processed 674584 reads in 246.473 CPU sec, 7.911 real sec
[M::mem_process_seqs] Processed 674478 reads in 256.203 CPU sec, 8.245 real sec
sorting block 136, #reads = 1078851 mem = 536877516 peak = 1223946240
[M::process] read 674468 sequences (100000192 bp) in 2.236 CPU sec, 2.427 real sec...
sorting block 137, #reads = 1079681 mem = 536877653 peak = 1223946240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284595, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (268.54, 142.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 101, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (78.88, 61.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674416 sequences (100000286 bp) in 2.061 CPU sec, 2.111 real sec...
[M::process] read 674402 sequences (100000043 bp) in 1.691 CPU sec, 1.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283687, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (268.28, 141.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 101, 101)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285)
[M::mem_pestat] mean and std.dev: (49.64, 41.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 377)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674592 reads in 264.766 CPU sec, 8.504 real sec
[M::process] read 674466 sequences (100000233 bp) in 1.657 CPU sec, 1.663 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283751, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (270.12, 143.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674416 reads in 137.202 CPU sec, 4.517 real sec
sorting block 138, #reads = 1078575 mem = 536877336 peak = 1223946240
[M::mem_process_seqs] Processed 674468 reads in 328.761 CPU sec, 10.699 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283763, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (270.76, 143.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 73, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (95.64, 73.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 660)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674556 sequences (100000167 bp) in 2.236 CPU sec, 2.502 real sec...
[M::process] read 674992 sequences (100000041 bp) in 1.712 CPU sec, 1.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284279, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (270.21, 143.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674402 reads in 276.308 CPU sec, 8.891 real sec
sorting block 139, #reads = 1079414 mem = 536878800 peak = 1223946240
[M::mem_process_seqs] Processed 674466 reads in 152.467 CPU sec, 4.876 real sec
sorting block 140, #reads = 1079127 mem = 536878377 peak = 1223946240
[M::process] read 674874 sequences (100000243 bp) in 2.024 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283925, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.23, 137.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (53, 180, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (115.55, 82.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674910 sequences (100000206 bp) in 1.693 CPU sec, 1.717 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284980, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.65, 133.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284395, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.55, 132.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674556 reads in 369.998 CPU sec, 11.894 real sec
[M::mem_process_seqs] Processed 674992 reads in 253.983 CPU sec, 8.190 real sec
[M::mem_process_seqs] Processed 674874 reads in 263.526 CPU sec, 8.516 real sec
sorting block 141, #reads = 1079139 mem = 536880098 peak = 1223946240
[M::process] read 674896 sequences (100000229 bp) in 2.043 CPU sec, 2.364 real sec...
sorting block 142, #reads = 1080618 mem = 536880742 peak = 1223946240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285318, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (258.44, 133.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 218, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (159.67, 133.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675028 sequences (100000026 bp) in 2.145 CPU sec, 2.250 real sec...
[M::process] read 674866 sequences (100000293 bp) in 1.731 CPU sec, 1.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284974, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.81, 133.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 183, 183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375)
[M::mem_pestat] mean and std.dev: (117.56, 75.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674910 reads in 249.827 CPU sec, 8.053 real sec
[M::mem_process_seqs] Processed 674896 reads in 137.337 CPU sec, 4.367 real sec
sorting block 143, #reads = 1080635 mem = 536877610 peak = 1223946240
[M::process] read 674990 sequences (100000196 bp) in 2.154 CPU sec, 2.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284311, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.36, 131.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675050 sequences (100000297 bp) in 1.687 CPU sec, 1.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284368, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (259.03, 133.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284850, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.50, 133.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 202, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1675)
[M::mem_pestat] mean and std.dev: (318.00, 330.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2230)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675028 reads in 352.763 CPU sec, 11.330 real sec
[M::mem_process_seqs] Processed 674866 reads in 242.454 CPU sec, 7.807 real sec
[M::mem_process_seqs] Processed 674990 reads in 252.199 CPU sec, 8.141 real sec
sorting block 144, #reads = 1080071 mem = 536880748 peak = 1223946240
[M::process] read 674994 sequences (100000065 bp) in 2.132 CPU sec, 2.336 real sec...
sorting block 145, #reads = 1079814 mem = 536879015 peak = 1223946240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284697, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.92, 132.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674928 sequences (100000081 bp) in 2.001 CPU sec, 2.180 real sec...
[M::process] read 674778 sequences (100000161 bp) in 1.607 CPU sec, 1.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284784, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.89, 131.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675050 reads in 235.301 CPU sec, 7.553 real sec
[M::mem_process_seqs] Processed 674994 reads in 128.383 CPU sec, 4.083 real sec
sorting block 146, #reads = 1080242 mem = 536878688 peak = 1223946240
[M::process] read 674738 sequences (100000083 bp) in 1.949 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284535, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.93, 133.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674792 sequences (100000014 bp) in 1.685 CPU sec, 1.692 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284931, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.34, 134.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285024, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.17, 136.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 104, 602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1720)
[M::mem_pestat] mean and std.dev: (270.56, 368.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2279)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674928 reads in 319.814 CPU sec, 10.299 real sec
[M::mem_process_seqs] Processed 674778 reads in 223.887 CPU sec, 7.187 real sec
[M::mem_process_seqs] Processed 674738 reads in 232.583 CPU sec, 7.487 real sec
sorting block 147, #reads = 1079579 mem = 536878284 peak = 1223946240
[M::process] read 674862 sequences (100000038 bp) in 1.951 CPU sec, 2.271 real sec...
sorting block 148, #reads = 1079073 mem = 536876240 peak = 1223946240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284973, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (262.75, 138.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675060 sequences (100000288 bp) in 2.025 CPU sec, 2.285 real sec...
[M::process] read 675418 sequences (100000165 bp) in 1.506 CPU sec, 1.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285131, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (263.66, 140.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674792 reads in 223.053 CPU sec, 7.196 real sec
[M::mem_process_seqs] Processed 674862 reads in 123.696 CPU sec, 3.947 real sec
sorting block 149, #reads = 1080225 mem = 536877549 peak = 1223946240
[M::process] read 675212 sequences (100000198 bp) in 2.155 CPU sec, 2.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285479, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.10, 136.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 18, 99)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261)
[M::mem_pestat] mean and std.dev: (49.42, 59.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 342)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675136 sequences (100000206 bp) in 1.530 CPU sec, 1.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285056, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.07, 127.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285290, 11, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.43, 129.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 104, 163)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (84.27, 69.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (672, 672, 672)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (672, 672)
[M::mem_pestat] mean and std.dev: (672.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (672, 672)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675060 reads in 319.047 CPU sec, 10.260 real sec
[M::mem_process_seqs] Processed 675418 reads in 220.233 CPU sec, 7.096 real sec
[M::mem_process_seqs] Processed 675212 reads in 229.856 CPU sec, 7.437 real sec
sorting block 150, #reads = 1080539 mem = 536879091 peak = 1223946240
[M::process] read 674910 sequences (100000185 bp) in 2.050 CPU sec, 2.331 real sec...
sorting block 151, #reads = 1080125 mem = 536877511 peak = 1223946240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285647, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.94, 130.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 278, 1522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4562)
[M::mem_pestat] mean and std.dev: (503.47, 630.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6082)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674976 sequences (100000192 bp) in 2.113 CPU sec, 2.452 real sec...
[M::process] read 674914 sequences (100000285 bp) in 1.590 CPU sec, 1.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284907, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.91, 129.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 23, 3667)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10973)
[M::mem_pestat] mean and std.dev: (1420.46, 1776.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14626)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675136 reads in 215.556 CPU sec, 7.002 real sec
[M::mem_process_seqs] Processed 674910 reads in 121.162 CPU sec, 3.874 real sec
sorting block 152, #reads = 1080106 mem = 536880074 peak = 1223946240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284532, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.26, 129.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674816 sequences (100000065 bp) in 2.030 CPU sec, 2.433 real sec...
[M::process] read 674932 sequences (100000068 bp) in 1.412 CPU sec, 1.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284740, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.99, 129.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674976 reads in 200.875 CPU sec, 6.483 real sec
[M::mem_process_seqs] Processed 674914 reads in 122.526 CPU sec, 3.927 real sec
sorting block 153, #reads = 1078714 mem = 536879282 peak = 1223946240
sorting block 154, #reads = 1079077 mem = 536877198 peak = 1223946240
[M::process] read 674948 sequences (100000043 bp) in 2.153 CPU sec, 2.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284919, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.44, 131.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674936 sequences (100000275 bp) in 1.514 CPU sec, 1.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284500, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.49, 132.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674816 reads in 218.403 CPU sec, 7.046 real sec
[M::mem_process_seqs] Processed 674932 reads in 121.527 CPU sec, 3.897 real sec
sorting block 155, #reads = 1080124 mem = 536876751 peak = 1223946240
[M::process] read 674808 sequences (100000256 bp) in 2.028 CPU sec, 2.137 real sec...
[M::process] read 674872 sequences (100000043 bp) in 1.553 CPU sec, 1.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284696, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.11, 133.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674936 reads in 111.303 CPU sec, 3.561 real sec
sorting block 156, #reads = 1077946 mem = 536878629 peak = 1223946240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285401, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.53, 132.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284522, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674898 sequences (100000187 bp) in 1.292 CPU sec, 1.323 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (259.93, 133.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 74, 668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1858)
[M::mem_pestat] mean and std.dev: (290.00, 395.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2453)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674948 reads in 294.501 CPU sec, 9.450 real sec
[M::mem_process_seqs] Processed 674808 reads in 202.948 CPU sec, 6.506 real sec
sorting block 157, #reads = 1078042 mem = 536877112 peak = 1223946240
[M::process] read 674962 sequences (100000241 bp) in 2.183 CPU sec, 2.408 real sec...
[M::process] read 674970 sequences (100000124 bp) in 1.468 CPU sec, 1.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285235, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.87, 132.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 2, 11)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 29)
[M::mem_pestat] mean and std.dev: (5.27, 4.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674898 reads in 100.330 CPU sec, 3.219 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284790, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (258.71, 132.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674776 sequences (100000085 bp) in 1.452 CPU sec, 1.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284977, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (259.10, 133.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (606, 651, 798)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (222, 1182)
[M::mem_pestat] mean and std.dev: (635.20, 121.36)
[M::mem_pestat] low and high boundaries for proper pairs: (30, 1374)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674872 reads in 303.793 CPU sec, 9.777 real sec
sorting block 158, #reads = 1075655 mem = 536880070 peak = 1223946240
[M::mem_process_seqs] Processed 674962 reads in 206.578 CPU sec, 6.638 real sec
sorting block 159, #reads = 1077778 mem = 536878271 peak = 1223946240
[M::process] read 674784 sequences (100000029 bp) in 2.187 CPU sec, 2.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285083, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.07, 133.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674704 sequences (100000292 bp) in 1.318 CPU sec, 1.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285335, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.90, 131.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285645, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.12, 132.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674970 reads in 300.180 CPU sec, 9.673 real sec
[M::mem_process_seqs] Processed 674776 reads in 205.140 CPU sec, 6.594 real sec
[M::mem_process_seqs] Processed 674784 reads in 214.636 CPU sec, 6.923 real sec
sorting block 160, #reads = 1078803 mem = 536879613 peak = 1223946240
[M::process] read 674470 sequences (100000226 bp) in 2.176 CPU sec, 2.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285488, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.72, 136.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 60, 923)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2749)
[M::mem_pestat] mean and std.dev: (244.46, 372.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3662)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 161, #reads = 1076708 mem = 536880592 peak = 1223946240
[M::process] read 674584 sequences (100000237 bp) in 2.064 CPU sec, 2.176 real sec...
[M::process] read 674418 sequences (100000063 bp) in 1.464 CPU sec, 1.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285277, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.41, 137.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674704 reads in 210.045 CPU sec, 6.731 real sec
[M::mem_process_seqs] Processed 674470 reads in 121.724 CPU sec, 3.869 real sec
sorting block 162, #reads = 1076946 mem = 536877731 peak = 1223946240
[M::process] read 674434 sequences (100000211 bp) in 2.098 CPU sec, 2.234 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284986, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.91, 139.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674726 sequences (100000065 bp) in 1.562 CPU sec, 1.603 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284473, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.10, 139.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 39, 1602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4796)
[M::mem_pestat] mean and std.dev: (684.15, 915.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6393)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284639, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (267.82, 140.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674584 reads in 314.316 CPU sec, 10.129 real sec
[M::mem_process_seqs] Processed 674434 reads in 220.218 CPU sec, 7.087 real sec
[M::mem_process_seqs] Processed 674418 reads in 228.744 CPU sec, 7.380 real sec
sorting block 163, #reads = 1080074 mem = 536879823 peak = 1223946240
[M::process] read 674482 sequences (100000290 bp) in 2.436 CPU sec, 2.627 real sec...
sorting block 164, #reads = 1079989 mem = 536879545 peak = 1223946240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284497, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (267.41, 140.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674514 sequences (100000181 bp) in 1.794 CPU sec, 1.854 real sec...
[M::process] read 674606 sequences (100000222 bp) in 1.493 CPU sec, 1.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283912, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (270.47, 143.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674726 reads in 219.271 CPU sec, 7.047 real sec
[M::mem_process_seqs] Processed 674482 reads in 121.722 CPU sec, 3.903 real sec
sorting block 165, #reads = 1080225 mem = 536876909 peak = 1223946240
[M::process] read 674614 sequences (100000151 bp) in 2.298 CPU sec, 2.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284799, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (270.72, 144.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 59, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1227)
[M::mem_pestat] mean and std.dev: (276.93, 359.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1715)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674490 sequences (100000027 bp) in 1.474 CPU sec, 1.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284121, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (269.60, 144.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 104, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1613)
[M::mem_pestat] mean and std.dev: (366.68, 564.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2624)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284502, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (270.12, 144.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 598, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1816)
[M::mem_pestat] mean and std.dev: (474.00, 280.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2358)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674514 reads in 333.760 CPU sec, 10.721 real sec
[M::mem_process_seqs] Processed 674606 reads in 227.698 CPU sec, 7.342 real sec
[M::mem_process_seqs] Processed 674614 reads in 236.471 CPU sec, 7.647 real sec
sorting block 166, #reads = 1077803 mem = 536881308 peak = 1223946240
[M::process] read 674568 sequences (100000193 bp) in 2.007 CPU sec, 2.178 real sec...
sorting block 167, #reads = 1079187 mem = 536881563 peak = 1223946240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284308, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (270.86, 145.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674486 sequences (100000077 bp) in 2.257 CPU sec, 2.461 real sec...
[M::process] read 674508 sequences (100000083 bp) in 1.423 CPU sec, 1.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284262, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (270.77, 144.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674490 reads in 222.852 CPU sec, 7.180 real sec
[M::mem_process_seqs] Processed 674568 reads in 122.535 CPU sec, 3.948 real sec
sorting block 168, #reads = 1079663 mem = 536880350 peak = 1223946240
[M::process] read 674954 sequences (100000159 bp) in 2.024 CPU sec, 2.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284131, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (272.52, 146.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675052 sequences (100000077 bp) in 1.452 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283909, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (270.54, 143.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674486 reads in 227.465 CPU sec, 7.329 real sec
[M::mem_process_seqs] Processed 674508 reads in 125.526 CPU sec, 4.029 real sec
sorting block 169, #reads = 1079300 mem = 536876438 peak = 1223946240
[M::process] read 675130 sequences (100000224 bp) in 2.123 CPU sec, 2.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284629, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (261.26, 136.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675010 sequences (100000140 bp) in 1.571 CPU sec, 1.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285086, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.68, 135.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284869, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.22, 133.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674954 reads in 320.110 CPU sec, 10.292 real sec
sorting block 170, #reads = 1080038 mem = 536876546 peak = 1223946240
[M::mem_process_seqs] Processed 675052 reads in 221.933 CPU sec, 7.132 real sec
[M::mem_process_seqs] Processed 675130 reads in 230.049 CPU sec, 7.412 real sec
sorting block 171, #reads = 1080055 mem = 536885161 peak = 1223946240
[M::process] read 674954 sequences (100000272 bp) in 2.033 CPU sec, 2.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284511, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.46, 133.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674922 sequences (100000000 bp) in 2.104 CPU sec, 2.252 real sec...
[M::process] read 674978 sequences (100000041 bp) in 1.501 CPU sec, 1.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285410, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.71, 133.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675010 reads in 228.293 CPU sec, 7.365 real sec
[M::mem_process_seqs] Processed 674954 reads in 127.197 CPU sec, 4.066 real sec
sorting block 172, #reads = 1080754 mem = 536879052 peak = 1223946240
sorting block 173, #reads = 1079529 mem = 536877689 peak = 1223946240
[M::process] read 674988 sequences (100000100 bp) in 2.227 CPU sec, 2.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284717, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (260.16, 136.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675140 sequences (100000270 bp) in 1.460 CPU sec, 1.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285006, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.80, 134.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285191, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.17, 134.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 546, 2480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7324)
[M::mem_pestat] mean and std.dev: (919.62, 1116.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9746)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674922 reads in 305.903 CPU sec, 9.846 real sec
[M::mem_process_seqs] Processed 674978 reads in 208.195 CPU sec, 6.696 real sec
[M::mem_process_seqs] Processed 674988 reads in 217.642 CPU sec, 7.023 real sec
sorting block 174, #reads = 1080603 mem = 536879535 peak = 1223946240
[M::process] read 675094 sequences (100000296 bp) in 2.162 CPU sec, 2.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285456, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (253.96, 131.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674996 sequences (100000285 bp) in 2.096 CPU sec, 2.276 real sec...
[M::process] read 675060 sequences (100000102 bp) in 1.524 CPU sec, 1.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284855, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (256.48, 133.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675140 reads in 224.711 CPU sec, 7.223 real sec
sorting block 175, #reads = 1077425 mem = 536879389 peak = 1223946240
[M::mem_process_seqs] Processed 675094 reads in 126.990 CPU sec, 4.039 real sec
sorting block 176, #reads = 1079594 mem = 536878129 peak = 1228390400
[M::process] read 675094 sequences (100000119 bp) in 2.207 CPU sec, 2.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284964, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (256.54, 134.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675012 sequences (100000189 bp) in 1.587 CPU sec, 1.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284881, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (256.51, 135.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284573, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (259.17, 137.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674996 reads in 317.763 CPU sec, 10.221 real sec
[M::mem_process_seqs] Processed 675060 reads in 220.733 CPU sec, 7.094 real sec
[M::mem_process_seqs] Processed 675094 reads in 229.914 CPU sec, 7.442 real sec
sorting block 177, #reads = 1079437 mem = 536880622 peak = 1228390400
[M::process] read 675134 sequences (100000240 bp) in 2.390 CPU sec, 2.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285485, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (261.34, 139.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 178, #reads = 1079376 mem = 536879028 peak = 1231683584
[M::process] read 675452 sequences (100000224 bp) in 2.048 CPU sec, 2.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285439, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (258.74, 137.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675526 sequences (100000082 bp) in 1.460 CPU sec, 1.504 real sec...
[M::mem_process_seqs] Processed 675012 reads in 204.807 CPU sec, 6.579 real sec
[M::mem_process_seqs] Processed 675134 reads in 116.973 CPU sec, 3.735 real sec
sorting block 179, #reads = 1078946 mem = 536876891 peak = 1231683584
[M::process] read 675318 sequences (100000100 bp) in 2.256 CPU sec, 2.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285220, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.47, 129.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675242 sequences (100000184 bp) in 1.514 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285547, 27, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.24, 131.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 25, 1586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4748)
[M::mem_pestat] mean and std.dev: (497.15, 708.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6329)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284973, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (253.93, 130.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 42, 970)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2902)
[M::mem_pestat] mean and std.dev: (389.07, 501.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3868)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675452 reads in 308.790 CPU sec, 9.924 real sec
[M::mem_process_seqs] Processed 675318 reads in 217.356 CPU sec, 6.990 real sec
[M::mem_process_seqs] Processed 675526 reads in 226.826 CPU sec, 7.322 real sec
sorting block 180, #reads = 1077843 mem = 536879114 peak = 1231683584
[M::process] read 675102 sequences (100000093 bp) in 2.377 CPU sec, 2.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286128, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.04, 132.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 577, 1966)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5518)
[M::mem_pestat] mean and std.dev: (802.30, 783.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7294)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 181, #reads = 1080034 mem = 536877719 peak = 1231683584
[M::process] read 675012 sequences (100000101 bp) in 2.055 CPU sec, 2.348 real sec...
[M::process] read 674902 sequences (100000219 bp) in 1.495 CPU sec, 1.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285423, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.47, 131.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675242 reads in 211.038 CPU sec, 6.800 real sec
[M::mem_process_seqs] Processed 675102 reads in 119.755 CPU sec, 3.834 real sec
sorting block 182, #reads = 1080590 mem = 536878934 peak = 1231683584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285383, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.87, 131.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 61, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (111.40, 173.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674874 sequences (100000155 bp) in 2.237 CPU sec, 2.455 real sec...
[M::process] read 674938 sequences (100000210 bp) in 1.354 CPU sec, 1.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285561, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.42, 130.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675012 reads in 208.131 CPU sec, 6.724 real sec
sorting block 183, #reads = 1079123 mem = 536878917 peak = 1231683584
[M::process] read 674838 sequences (100000187 bp) in 1.546 CPU sec, 1.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285698, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.60, 130.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 957, 993)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2953)
[M::mem_pestat] mean and std.dev: (619.06, 503.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3933)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674874 reads in 114.077 CPU sec, 3.788 real sec
[M::mem_process_seqs] Processed 674902 reads in 267.985 CPU sec, 8.766 real sec
sorting block 184, #reads = 1080439 mem = 536880401 peak = 1231683584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285498, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (259.43, 132.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 71, 184)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (83.43, 72.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674790 sequences (100000062 bp) in 2.154 CPU sec, 2.330 real sec...
[M::process] read 674872 sequences (100000175 bp) in 1.527 CPU sec, 1.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285251, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (259.59, 133.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 22, 605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1795)
[M::mem_pestat] mean and std.dev: (168.00, 261.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2390)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674938 reads in 227.009 CPU sec, 7.261 real sec
[M::mem_process_seqs] Processed 674838 reads in 125.627 CPU sec, 4.014 real sec
sorting block 185, #reads = 1078378 mem = 536881141 peak = 1231683584
[M::process] read 674962 sequences (100000042 bp) in 1.978 CPU sec, 2.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284895, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (260.95, 135.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675084 sequences (100000175 bp) in 1.488 CPU sec, 1.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284701, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (258.69, 132.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285189, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (258.16, 132.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674790 reads in 316.895 CPU sec, 10.207 real sec
[M::mem_process_seqs] Processed 674872 reads in 223.334 CPU sec, 7.180 real sec
[M::mem_process_seqs] Processed 674962 reads in 232.140 CPU sec, 7.485 real sec
sorting block 186, #reads = 1079117 mem = 536880787 peak = 1231683584
sorting block 187, #reads = 1080108 mem = 536880618 peak = 1231683584
[M::process] read 674906 sequences (100000059 bp) in 2.306 CPU sec, 2.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285219, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (258.92, 133.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674766 sequences (100000267 bp) in 2.206 CPU sec, 2.348 real sec...
[M::process] read 674704 sequences (100000084 bp) in 1.512 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285310, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (257.95, 131.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675084 reads in 209.674 CPU sec, 6.717 real sec
[M::mem_process_seqs] Processed 674906 reads in 120.157 CPU sec, 3.831 real sec
sorting block 188, #reads = 1078675 mem = 536878561 peak = 1231683584
[M::process] read 674672 sequences (100000193 bp) in 2.256 CPU sec, 2.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284975, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.11, 134.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 555, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1649)
[M::mem_pestat] mean and std.dev: (481.45, 549.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2677)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674754 sequences (100000284 bp) in 1.462 CPU sec, 1.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285071, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.17, 136.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 58, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (105.64, 84.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674766 reads in 200.338 CPU sec, 6.430 real sec
sorting block 189, #reads = 1077258 mem = 536879492 peak = 1231683584
[M::process] read 674630 sequences (100000189 bp) in 1.540 CPU sec, 1.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285079, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (264.99, 138.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 104, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276)
[M::mem_pestat] mean and std.dev: (59.09, 42.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 362)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674672 reads in 121.418 CPU sec, 4.053 real sec
sorting block 190, #reads = 1074003 mem = 536877337 peak = 1231683584
[M::mem_process_seqs] Processed 674704 reads in 271.170 CPU sec, 8.881 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285794, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.15, 140.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 205, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1949)
[M::mem_pestat] mean and std.dev: (470.13, 524.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2593)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674814 sequences (100000227 bp) in 2.261 CPU sec, 2.457 real sec...
[M::process] read 674620 sequences (100000040 bp) in 1.523 CPU sec, 1.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284995, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (267.19, 141.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674754 reads in 230.600 CPU sec, 7.392 real sec
[M::mem_process_seqs] Processed 674630 reads in 128.477 CPU sec, 4.102 real sec
sorting block 191, #reads = 1071266 mem = 536879760 peak = 1231683584
sorting block 192, #reads = 1078380 mem = 536877978 peak = 1231683584
[M::process] read 674548 sequences (100000053 bp) in 2.070 CPU sec, 2.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285020, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (268.36, 142.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 17, 701)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2097)
[M::mem_pestat] mean and std.dev: (194.54, 335.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2795)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674636 sequences (100000003 bp) in 1.427 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284155, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (266.81, 141.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284491, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (269.49, 144.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674814 reads in 313.604 CPU sec, 10.072 real sec
[M::mem_process_seqs] Processed 674620 reads in 221.022 CPU sec, 7.104 real sec
[M::mem_process_seqs] Processed 674548 reads in 229.864 CPU sec, 7.409 real sec
sorting block 193, #reads = 1080323 mem = 536880294 peak = 1231683584
[M::process] read 674474 sequences (100000065 bp) in 2.609 CPU sec, 2.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285075, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (269.81, 144.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 217, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (179.00, 146.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674520 sequences (100000051 bp) in 2.380 CPU sec, 2.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284673, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (268.33, 142.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674636 reads in 211.605 CPU sec, 6.777 real sec
sorting block 194, #reads = 1080239 mem = 536878700 peak = 1231683584
[M::mem_process_seqs] Processed 674474 reads in 122.768 CPU sec, 3.886 real sec
[M::process] read 674598 sequences (100000087 bp) in 1.773 CPU sec, 1.796 real sec...
sorting block 195, #reads = 1073495 mem = 536879676 peak = 1231683584
[M::process] read 674680 sequences (100000200 bp) in 2.280 CPU sec, 2.425 real sec...
[M::process] read 674692 sequences (100000270 bp) in 1.510 CPU sec, 1.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283782, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (269.64, 144.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 531, 6943)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20707)
[M::mem_pestat] mean and std.dev: (1921.33, 2823.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 27589)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674598 reads in 122.133 CPU sec, 3.902 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284428, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (268.99, 143.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674822 sequences (100000152 bp) in 1.605 CPU sec, 1.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284332, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (268.96, 143.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674520 reads in 350.079 CPU sec, 11.208 real sec
[M::mem_process_seqs] Processed 674680 reads in 246.819 CPU sec, 7.902 real sec
sorting block 196, #reads = 1079637 mem = 536878285 peak = 1231683584
sorting block 197, #reads = 1080026 mem = 536882132 peak = 1231683584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284296, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (269.93, 145.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 94, 1607)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4791)
[M::mem_pestat] mean and std.dev: (328.91, 521.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6383)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674936 sequences (100000208 bp) in 2.603 CPU sec, 2.823 real sec...
[M::process] read 675162 sequences (100000075 bp) in 1.549 CPU sec, 1.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284344, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.33, 141.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 73, 672)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870)
[M::mem_pestat] mean and std.dev: (295.36, 397.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2469)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674692 reads in 224.667 CPU sec, 7.206 real sec
[M::mem_process_seqs] Processed 674822 reads in 123.741 CPU sec, 3.953 real sec
sorting block 198, #reads = 1080183 mem = 536879251 peak = 1231683584
[M::process] read 675024 sequences (100000251 bp) in 2.225 CPU sec, 2.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285041, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.13, 134.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 273, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (208.81, 154.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675060 sequences (100000195 bp) in 1.404 CPU sec, 1.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285270, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.79, 134.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284780, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.78, 134.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674936 reads in 308.393 CPU sec, 9.893 real sec
[M::mem_process_seqs] Processed 675162 reads in 214.897 CPU sec, 6.922 real sec
[M::mem_process_seqs] Processed 675024 reads in 223.247 CPU sec, 7.210 real sec
sorting block 199, #reads = 1080410 mem = 536878537 peak = 1231683584
[M::process] read 675040 sequences (100000266 bp) in 2.393 CPU sec, 2.582 real sec...
sorting block 200, #reads = 1080779 mem = 536878029 peak = 1231683584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284880, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.87, 134.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674986 sequences (100000263 bp) in 1.831 CPU sec, 1.888 real sec...
[M::process] read 674970 sequences (100000103 bp) in 1.477 CPU sec, 1.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284952, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.00, 134.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 10, 24)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 52)
[M::mem_pestat] mean and std.dev: (13.27, 6.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 66)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675060 reads in 211.336 CPU sec, 6.737 real sec
[M::mem_process_seqs] Processed 675040 reads in 117.749 CPU sec, 3.731 real sec
sorting block 201, #reads = 1080558 mem = 536877201 peak = 1231683584
[M::process] read 674844 sequences (100000056 bp) in 2.125 CPU sec, 2.234 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285064, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.36, 134.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674846 sequences (100000231 bp) in 1.460 CPU sec, 1.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284799, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.58, 136.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 157, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289)
[M::mem_pestat] mean and std.dev: (341.70, 382.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1874)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284701, 13, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.08, 135.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 329, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (210.38, 144.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674986 reads in 277.762 CPU sec, 8.886 real sec
[M::mem_process_seqs] Processed 674970 reads in 191.771 CPU sec, 6.160 real sec
[M::mem_process_seqs] Processed 674844 reads in 200.110 CPU sec, 6.446 real sec
sorting block 202, #reads = 1080170 mem = 536880733 peak = 1231683584
sorting block 203, #reads = 1078880 mem = 536879921 peak = 1231683584
[M::process] read 674972 sequences (100000239 bp) in 2.356 CPU sec, 2.501 real sec...
[M::process] read 675042 sequences (100000121 bp) in 1.798 CPU sec, 1.843 real sec...
[M::process] read 675044 sequences (100000107 bp) in 1.530 CPU sec, 1.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284891, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.17, 134.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674972 reads in 109.727 CPU sec, 3.478 real sec
sorting block 204, #reads = 1079621 mem = 536880394 peak = 1231683584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285001, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.47, 133.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 27, 85)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 201)
[M::mem_pestat] mean and std.dev: (31.89, 20.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 259)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675018 sequences (100000102 bp) in 1.749 CPU sec, 1.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285228, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.63, 134.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 32, 4025)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12047)
[M::mem_pestat] mean and std.dev: (1191.44, 1757.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16058)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674846 reads in 307.688 CPU sec, 9.799 real sec
[M::mem_process_seqs] Processed 675042 reads in 118.701 CPU sec, 3.799 real sec
sorting block 205, #reads = 1079710 mem = 536879436 peak = 1231683584
[M::process] read 674962 sequences (100000132 bp) in 2.197 CPU sec, 2.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285465, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.70, 135.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674962 sequences (100000159 bp) in 1.520 CPU sec, 1.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284725, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (258.05, 135.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285716, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (260.72, 138.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675044 reads in 269.469 CPU sec, 8.728 real sec
[M::mem_process_seqs] Processed 675018 reads in 185.026 CPU sec, 5.941 real sec
[M::mem_process_seqs] Processed 674962 reads in 194.716 CPU sec, 6.276 real sec
sorting block 206, #reads = 1079287 mem = 536877437 peak = 1231683584
[M::process] read 675256 sequences (100000234 bp) in 2.421 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285266, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (259.56, 138.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 1050, 2103)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6013)
[M::mem_pestat] mean and std.dev: (1163.09, 927.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7968)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 207, #reads = 1072046 mem = 536880130 peak = 1231683584
[M::process] read 675330 sequences (100000171 bp) in 1.743 CPU sec, 1.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285177, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (255.62, 132.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675186 sequences (100000215 bp) in 1.480 CPU sec, 1.484 real sec...
[M::mem_process_seqs] Processed 674962 reads in 186.490 CPU sec, 5.931 real sec
[M::mem_process_seqs] Processed 675256 reads in 107.243 CPU sec, 3.380 real sec
sorting block 208, #reads = 1076627 mem = 536881442 peak = 1231683584
[M::process] read 675240 sequences (100000188 bp) in 2.000 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284850, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (251.86, 127.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 110, 1121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3341)
[M::mem_pestat] mean and std.dev: (428.10, 491.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4451)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675118 sequences (100000225 bp) in 1.120 CPU sec, 1.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285247, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.69, 131.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 13, 13)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 13)
[M::mem_pestat] mean and std.dev: (13.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (13, 13)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285579, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.51, 128.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 128, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1338)
[M::mem_pestat] mean and std.dev: (317.42, 438.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2070)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675330 reads in 284.819 CPU sec, 9.134 real sec
[M::mem_process_seqs] Processed 675240 reads in 196.686 CPU sec, 6.339 real sec
[M::mem_process_seqs] Processed 675186 reads in 204.610 CPU sec, 6.613 real sec
sorting block 209, #reads = 1079651 mem = 536876908 peak = 1231683584
[M::process] read 675040 sequences (100000172 bp) in 2.103 CPU sec, 2.312 real sec...
sorting block 210, #reads = 1080116 mem = 536881911 peak = 1231683584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284883, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.76, 131.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 157, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (188.11, 157.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675008 sequences (100000266 bp) in 1.580 CPU sec, 1.622 real sec...
[M::process] read 674912 sequences (100000199 bp) in 1.510 CPU sec, 1.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284541, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (258.18, 131.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675118 reads in 200.264 CPU sec, 6.389 real sec
[M::mem_process_seqs] Processed 675040 reads in 110.640 CPU sec, 3.514 real sec
sorting block 211, #reads = 1079299 mem = 536879252 peak = 1231683584
[M::process] read 675030 sequences (100000173 bp) in 2.108 CPU sec, 2.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285079, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.08, 131.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675034 sequences (100000272 bp) in 1.170 CPU sec, 1.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285299, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.03, 131.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 57, 920)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2736)
[M::mem_pestat] mean and std.dev: (199.67, 329.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3644)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285306, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (257.73, 131.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 183, 1741)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5171)
[M::mem_pestat] mean and std.dev: (524.67, 736.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6886)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675008 reads in 296.447 CPU sec, 9.503 real sec
[M::mem_process_seqs] Processed 674912 reads in 205.712 CPU sec, 6.628 real sec
[M::mem_process_seqs] Processed 675030 reads in 213.919 CPU sec, 6.912 real sec
sorting block 212, #reads = 1079838 mem = 536881332 peak = 1231683584
sorting block 213, #reads = 1080603 mem = 536876343 peak = 1231683584
[M::process] read 674920 sequences (100000177 bp) in 2.072 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284811, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.07, 129.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674928 sequences (100000151 bp) in 1.737 CPU sec, 1.786 real sec...
[M::process] read 674790 sequences (100000097 bp) in 1.650 CPU sec, 1.646 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284403, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.33, 130.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675034 reads in 209.507 CPU sec, 6.659 real sec
[M::mem_process_seqs] Processed 674920 reads in 115.829 CPU sec, 3.663 real sec
sorting block 214, #reads = 1080522 mem = 536879051 peak = 1231683584
[M::process] read 674736 sequences (100000248 bp) in 2.101 CPU sec, 2.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284900, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.22, 131.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674796 sequences (100000243 bp) in 1.650 CPU sec, 1.653 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284587, 25, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.31, 133.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 518, 1453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4309)
[M::mem_pestat] mean and std.dev: (630.40, 642.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5737)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284416, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.85, 131.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674928 reads in 307.919 CPU sec, 9.850 real sec
[M::mem_process_seqs] Processed 674736 reads in 214.753 CPU sec, 6.894 real sec
sorting block 215, #reads = 1079960 mem = 536878641 peak = 1231683584
[M::mem_process_seqs] Processed 674790 reads in 222.980 CPU sec, 7.174 real sec
sorting block 216, #reads = 1079536 mem = 536879957 peak = 1231683584
[M::process] read 674666 sequences (100000174 bp) in 2.318 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284768, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.16, 134.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 284, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (187.40, 186.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674716 sequences (100000120 bp) in 1.380 CPU sec, 1.373 real sec...
[M::process] read 674530 sequences (100000044 bp) in 1.170 CPU sec, 1.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284934, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.62, 134.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674796 reads in 199.246 CPU sec, 6.344 real sec
sorting block 217, #reads = 1079205 mem = 536878203 peak = 1231683584
[M::process] read 674656 sequences (100000019 bp) in 1.424 CPU sec, 1.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284065, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.59, 137.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674716 reads in 115.039 CPU sec, 3.702 real sec
[M::mem_process_seqs] Processed 674666 reads in 239.984 CPU sec, 7.687 real sec
sorting block 218, #reads = 1079731 mem = 536876427 peak = 1231683584
[M::process] read 674684 sequences (100000055 bp) in 2.014 CPU sec, 2.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284527, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.99, 138.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 305, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1364)
[M::mem_pestat] mean and std.dev: (277.23, 198.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1802)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674548 sequences (100000287 bp) in 1.550 CPU sec, 1.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285184, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.78, 138.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285182, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.56, 138.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 76, 101)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263)
[M::mem_pestat] mean and std.dev: (48.42, 41.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 344)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674530 reads in 319.708 CPU sec, 10.214 real sec
[M::mem_process_seqs] Processed 674656 reads in 213.346 CPU sec, 6.847 real sec
sorting block 219, #reads = 1076983 mem = 536881148 peak = 1231683584
[M::mem_process_seqs] Processed 674684 reads in 224.275 CPU sec, 7.219 real sec
sorting block 220, #reads = 1072173 mem = 536879093 peak = 1231683584
[M::process] read 674404 sequences (100000153 bp) in 2.292 CPU sec, 2.470 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284261, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.25, 139.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674464 sequences (100000147 bp) in 1.517 CPU sec, 1.595 real sec...
[M::process] read 674632 sequences (100000285 bp) in 1.520 CPU sec, 1.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284045, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (268.41, 140.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (63, 75, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (131.20, 140.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674548 reads in 221.726 CPU sec, 7.046 real sec
[M::mem_process_seqs] Processed 674404 reads in 126.733 CPU sec, 4.017 real sec
sorting block 221, #reads = 1074583 mem = 536877964 peak = 1231683584
[M::process] read 674514 sequences (100000162 bp) in 1.970 CPU sec, 2.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284112, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (268.14, 140.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674442 sequences (100000120 bp) in 1.590 CPU sec, 1.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284606, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (268.70, 142.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 14, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1482)
[M::mem_pestat] mean and std.dev: (304.36, 492.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2273)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284078, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (267.57, 140.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674464 reads in 323.057 CPU sec, 10.355 real sec
[M::mem_process_seqs] Processed 674514 reads in 228.110 CPU sec, 7.339 real sec
sorting block 222, #reads = 1078166 mem = 536878835 peak = 1231683584
[M::mem_process_seqs] Processed 674632 reads in 236.613 CPU sec, 7.636 real sec
sorting block 223, #reads = 1075496 mem = 536878622 peak = 1231683584
[M::process] read 674348 sequences (100000259 bp) in 2.373 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283865, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (269.41, 142.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 5, 32)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 90)
[M::mem_pestat] mean and std.dev: (13.91, 13.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 119)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674360 sequences (100000021 bp) in 1.673 CPU sec, 1.691 real sec...
[M::process] read 674504 sequences (100000195 bp) in 1.590 CPU sec, 1.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283469, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (269.30, 141.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674442 reads in 211.252 CPU sec, 6.719 real sec
[M::mem_process_seqs] Processed 674348 reads in 123.864 CPU sec, 3.914 real sec
sorting block 224, #reads = 1077536 mem = 536879400 peak = 1231683584
[M::process] read 674466 sequences (100000234 bp) in 2.081 CPU sec, 2.184 real sec...
[M::process] read 674560 sequences (100000283 bp) in 1.680 CPU sec, 1.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284487, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.90, 139.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 104, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272)
[M::mem_pestat] mean and std.dev: (71.07, 37.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 356)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674504 reads in 115.921 CPU sec, 3.714 real sec
sorting block 225, #reads = 1078205 mem = 536878383 peak = 1231683584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283166, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (270.23, 142.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674786 sequences (100000124 bp) in 1.201 CPU sec, 1.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283922, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (270.01, 143.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 233, 8627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25685)
[M::mem_pestat] mean and std.dev: (3112.39, 3943.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 34214)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674360 reads in 338.237 CPU sec, 10.791 real sec
[M::mem_process_seqs] Processed 674466 reads in 233.635 CPU sec, 7.480 real sec
sorting block 226, #reads = 1078754 mem = 536878592 peak = 1231683584
[M::process] read 674952 sequences (100000092 bp) in 2.139 CPU sec, 2.269 real sec...
[M::process] read 675046 sequences (100000226 bp) in 1.670 CPU sec, 1.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285281, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (258.19, 132.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674786 reads in 107.989 CPU sec, 3.446 real sec
sorting block 227, #reads = 1079249 mem = 536878656 peak = 1231683584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284315, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.86, 138.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674896 sequences (100000296 bp) in 1.623 CPU sec, 1.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284994, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (258.03, 132.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 776, 776)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1918)
[M::mem_pestat] mean and std.dev: (515.08, 347.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2489)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674560 reads in 320.278 CPU sec, 10.209 real sec
[M::mem_process_seqs] Processed 674952 reads in 225.934 CPU sec, 7.207 real sec
sorting block 228, #reads = 1077279 mem = 536880479 peak = 1231683584
[M::process] read 674850 sequences (100000282 bp) in 2.139 CPU sec, 2.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284776, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (259.06, 133.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 71, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (190.80, 186.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1377)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674966 sequences (100000233 bp) in 1.620 CPU sec, 1.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285120, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (259.24, 134.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 61, 180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418)
[M::mem_pestat] mean and std.dev: (95.16, 67.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 537)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284921, 30, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (259.54, 134.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 61, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1900)
[M::mem_pestat] mean and std.dev: (194.68, 343.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2513)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675046 reads in 316.001 CPU sec, 10.108 real sec
[M::mem_process_seqs] Processed 674896 reads in 218.020 CPU sec, 7.007 real sec
sorting block 229, #reads = 1079474 mem = 536879830 peak = 1231683584
[M::mem_process_seqs] Processed 674850 reads in 226.199 CPU sec, 7.285 real sec
sorting block 230, #reads = 1080945 mem = 536879399 peak = 1231683584
[M::process] read 674744 sequences (100000014 bp) in 2.251 CPU sec, 2.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284689, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (259.89, 134.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674892 sequences (100000186 bp) in 1.189 CPU sec, 1.287 real sec...
[M::process] read 674760 sequences (100000001 bp) in 1.230 CPU sec, 1.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284752, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.33, 134.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (958, 1966, 1966)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3982)
[M::mem_pestat] mean and std.dev: (1407.18, 696.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4990)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674966 reads in 216.346 CPU sec, 6.893 real sec
[M::mem_process_seqs] Processed 674744 reads in 119.048 CPU sec, 3.796 real sec
sorting block 231, #reads = 1079703 mem = 536881782 peak = 1231683584
[M::process] read 674738 sequences (100000181 bp) in 1.768 CPU sec, 1.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284931, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (260.73, 135.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674700 sequences (100000203 bp) in 1.220 CPU sec, 1.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284651, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.03, 136.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285159, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.18, 133.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (90, 215, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260)
[M::mem_pestat] mean and std.dev: (215.14, 176.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1650)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674892 reads in 306.730 CPU sec, 9.855 real sec
[M::mem_process_seqs] Processed 674760 reads in 211.253 CPU sec, 6.822 real sec
sorting block 232, #reads = 1080125 mem = 536877806 peak = 1231683584
[M::mem_process_seqs] Processed 674738 reads in 219.789 CPU sec, 7.118 real sec
sorting block 233, #reads = 1080243 mem = 536878277 peak = 1235202048
[M::process] read 674714 sequences (100000193 bp) in 2.342 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285094, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (259.65, 134.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 397, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1675)
[M::mem_pestat] mean and std.dev: (242.53, 240.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2226)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674758 sequences (100000161 bp) in 1.670 CPU sec, 1.663 real sec...
[M::process] read 674690 sequences (100000149 bp) in 1.670 CPU sec, 1.669 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284718, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (261.93, 136.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (365, 1362, 1362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3356)
[M::mem_pestat] mean and std.dev: (901.69, 591.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4353)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674700 reads in 212.382 CPU sec, 6.764 real sec
sorting block 234, #reads = 1080120 mem = 536881052 peak = 1235202048
[M::process] read 674748 sequences (100000125 bp) in 1.664 CPU sec, 1.673 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284714, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.35, 137.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 42, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 220)
[M::mem_pestat] mean and std.dev: (35.62, 23.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 285)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674758 reads in 117.440 CPU sec, 3.754 real sec
[M::mem_process_seqs] Processed 674714 reads in 252.366 CPU sec, 8.037 real sec
sorting block 235, #reads = 1080159 mem = 536878073 peak = 1235202048
[M::process] read 605180 sequences (89688813 bp) in 1.603 CPU sec, 1.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284638, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (264.68, 139.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 53, 1764)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5246)
[M::mem_pestat] mean and std.dev: (550.70, 794.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6987)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284846, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (265.18, 141.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 255780, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (265.44, 141.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674690 reads in 311.480 CPU sec, 10.017 real sec
[M::mem_process_seqs] Processed 674748 reads in 207.097 CPU sec, 6.686 real sec
sorting block 236, #reads = 1080131 mem = 536878311 peak = 1235202048
[M::mem_process_seqs] Processed 605180 reads in 214.819 CPU sec, 6.950 real sec
sorting block 237, #reads = 1079837 mem = 536875577 peak = 1246695424
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 254971880
overall: 70077845504 mem 42381.245 user 875.800 sys 1420.736 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003322.L002\tPU:L002\tSM:T21-D0003322\tLB:T21-D0003322\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 T21-D0003322_R1.fastq.gz T21-D0003322_R2.fastq.gz
[main] Real time: 1420.773 sec; CPU: 43257.048 sec
sorting block 238, #reads = 457759 mem = 227611281 peak = 1246695424
uniq header count 1
merging 239 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8689724698
output reads: 257393653
bam_mem_sort: 239 calls 147.719 user 2.176 sys 152.235 real
bam_write: 239 calls 108.540 user 6.257 sys 183.020 real
execute: 1 calls 56.390 user 2.176 sys 1488.120 real
merge_files: 1 calls 56.364 user 2.156 sys 66.951 real
parse_chunk: 3815 calls 179.519 user 40.908 sys 245.572 real
read_chunk: 10267 calls 85.851 user 57.533 sys 1385.338 real
sort_block: 1 calls 54.658 user 57.030 sys 1345.272 real
write_chunk: 6450 calls 56.002 user 1.862 sys 64.613 real
overall: 2891939840 mem 2497.550 user 139.213 sys 1488.140 real
ls: cannot access '*.cram': No such file or directory