cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2057.981 CPU sec, 72.061 real sec
[M::process] read 677422 sequences (100000179 bp) in 0.952 CPU sec, 0.955 real sec...
[M::process] read 677392 sequences (100000265 bp) in 1.488 CPU sec, 1.564 real sec...
[M::process] read 677174 sequences (100000220 bp) in 1.508 CPU sec, 1.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288899, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (220.13, 100.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677176 sequences (100000027 bp) in 1.426 CPU sec, 1.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288685, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (223.44, 104.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 289229, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (221.11, 101.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 173, 1288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3540)
[M::mem_pestat] mean and std.dev: (455.77, 467.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4666)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677422 reads in 296.715 CPU sec, 9.596 real sec
[M::mem_process_seqs] Processed 677174 reads in 190.753 CPU sec, 6.200 real sec
[M::mem_process_seqs] Processed 677392 reads in 198.780 CPU sec, 6.491 real sec
sorting block 0, #reads = 1082412 mem = 536876877 peak = 631939072
[M::process] read 677034 sequences (100000080 bp) in 1.758 CPU sec, 1.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288453, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (225.03, 105.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676734 sequences (100000020 bp) in 1.442 CPU sec, 1.530 real sec...
[M::process] read 676658 sequences (100000176 bp) in 1.386 CPU sec, 1.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288452, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.10, 109.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677176 reads in 189.261 CPU sec, 6.079 real sec
sorting block 1, #reads = 1082816 mem = 536877178 peak = 890728448
[M::mem_process_seqs] Processed 677034 reads in 106.464 CPU sec, 3.398 real sec
sorting block 2, #reads = 1082377 mem = 536879748 peak = 909176832
[M::process] read 676446 sequences (100000223 bp) in 1.890 CPU sec, 2.041 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288399, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.57, 109.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 39, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260)
[M::mem_pestat] mean and std.dev: (36.70, 32.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 345)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676456 sequences (100000174 bp) in 1.373 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288371, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.79, 113.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287637, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.16, 118.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676734 reads in 270.623 CPU sec, 8.792 real sec
[M::mem_process_seqs] Processed 676658 reads in 190.812 CPU sec, 6.240 real sec
[M::mem_process_seqs] Processed 676446 reads in 198.374 CPU sec, 6.514 real sec
sorting block 3, #reads = 1082203 mem = 536879150 peak = 909176832
[M::process] read 676316 sequences (100000045 bp) in 1.859 CPU sec, 2.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287900, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.39, 117.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1082332 mem = 536879001 peak = 909176832
[M::process] read 676322 sequences (100000109 bp) in 1.958 CPU sec, 2.106 real sec...
[M::process] read 676220 sequences (100000066 bp) in 1.394 CPU sec, 1.398 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288104, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.34, 116.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676456 reads in 192.012 CPU sec, 6.192 real sec
[M::mem_process_seqs] Processed 676316 reads in 106.783 CPU sec, 3.435 real sec
sorting block 5, #reads = 1082552 mem = 536879079 peak = 909176832
[M::process] read 676656 sequences (100000143 bp) in 1.852 CPU sec, 1.997 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287881, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.53, 118.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677444 sequences (100000017 bp) in 1.321 CPU sec, 1.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287668, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.41, 119.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288166, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.96, 114.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676322 reads in 271.517 CPU sec, 8.833 real sec
[M::mem_process_seqs] Processed 676220 reads in 190.098 CPU sec, 6.189 real sec
[M::mem_process_seqs] Processed 676656 reads in 198.389 CPU sec, 6.484 real sec
sorting block 6, #reads = 1082040 mem = 536879203 peak = 909176832
[M::process] read 677424 sequences (100000270 bp) in 2.072 CPU sec, 2.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288739, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (219.90, 99.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1081494 mem = 536878800 peak = 909176832
[M::process] read 677144 sequences (100000099 bp) in 1.852 CPU sec, 1.987 real sec...
[M::process] read 677168 sequences (100000292 bp) in 1.428 CPU sec, 1.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288537, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.87, 102.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677444 reads in 185.899 CPU sec, 6.004 real sec
[M::process] read 676816 sequences (100000188 bp) in 1.401 CPU sec, 1.417 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288569, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.38, 105.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677144 reads in 99.116 CPU sec, 3.246 real sec
sorting block 8, #reads = 1082608 mem = 536878393 peak = 909176832
[M::mem_process_seqs] Processed 677424 reads in 238.286 CPU sec, 7.766 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288271, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.40, 107.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676854 sequences (100000044 bp) in 1.760 CPU sec, 1.957 real sec...
sorting block 9, #reads = 1083158 mem = 536876641 peak = 924000256
[M::process] read 676640 sequences (100000285 bp) in 1.459 CPU sec, 1.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288549, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.27, 109.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677168 reads in 194.646 CPU sec, 6.370 real sec
[M::mem_process_seqs] Processed 676816 reads in 104.630 CPU sec, 3.381 real sec
sorting block 10, #reads = 1082325 mem = 536877080 peak = 924000256
[M::process] read 676466 sequences (100000019 bp) in 1.427 CPU sec, 1.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288250, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.33, 111.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 39, 81)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 175)
[M::mem_pestat] mean and std.dev: (48.00, 24.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 222)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676466 sequences (100000209 bp) in 1.306 CPU sec, 1.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287946, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.24, 116.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287995, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.86, 115.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676854 reads in 270.576 CPU sec, 8.759 real sec
[M::mem_process_seqs] Processed 676466 reads in 188.887 CPU sec, 6.143 real sec
[M::mem_process_seqs] Processed 676640 reads in 197.227 CPU sec, 6.431 real sec
sorting block 11, #reads = 1082471 mem = 536879969 peak = 924000256
[M::process] read 676356 sequences (100000165 bp) in 1.816 CPU sec, 2.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288074, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.44, 117.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 441, 2652)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7632)
[M::mem_pestat] mean and std.dev: (762.00, 979.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10122)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 12, #reads = 1082404 mem = 536878061 peak = 924000256
[M::process] read 676314 sequences (100000063 bp) in 1.685 CPU sec, 1.822 real sec...
[M::process] read 676148 sequences (100000019 bp) in 1.499 CPU sec, 1.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288201, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.30, 119.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676466 reads in 192.730 CPU sec, 6.334 real sec
[M::mem_process_seqs] Processed 676356 reads in 108.816 CPU sec, 3.545 real sec
sorting block 13, #reads = 1081802 mem = 536880671 peak = 924000256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288341, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.33, 120.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 76, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214)
[M::mem_pestat] mean and std.dev: (58.00, 29.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 276)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677268 sequences (100000205 bp) in 1.999 CPU sec, 2.198 real sec...
[M::process] read 677476 sequences (100000000 bp) in 1.441 CPU sec, 1.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288094, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.50, 120.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676314 reads in 178.763 CPU sec, 5.737 real sec
[M::mem_process_seqs] Processed 676148 reads in 100.043 CPU sec, 3.192 real sec
sorting block 14, #reads = 1082454 mem = 536878456 peak = 924000256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288805, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.18, 106.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677424 sequences (100000077 bp) in 1.826 CPU sec, 2.125 real sec...
[M::process] read 677038 sequences (100000076 bp) in 1.364 CPU sec, 1.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288958, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.71, 100.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 65, 867)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2511)
[M::mem_pestat] mean and std.dev: (441.92, 455.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3333)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677268 reads in 181.351 CPU sec, 5.862 real sec
[M::mem_process_seqs] Processed 677476 reads in 102.603 CPU sec, 3.301 real sec
sorting block 15, #reads = 1081820 mem = 536879836 peak = 924000256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288951, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.46, 102.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1083024 mem = 536880499 peak = 924000256
[M::process] read 677140 sequences (100000129 bp) in 2.079 CPU sec, 2.256 real sec...
[M::process] read 676904 sequences (100000257 bp) in 1.438 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288489, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.42, 104.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677424 reads in 180.807 CPU sec, 5.815 real sec
[M::process] read 676708 sequences (100000087 bp) in 1.430 CPU sec, 1.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288367, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.71, 109.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677140 reads in 97.898 CPU sec, 3.210 real sec
sorting block 17, #reads = 1082764 mem = 536880144 peak = 924000256
[M::mem_process_seqs] Processed 677038 reads in 233.054 CPU sec, 7.567 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287910, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.62, 111.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 106, 178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (98.17, 80.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 697)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676470 sequences (100000191 bp) in 2.040 CPU sec, 2.396 real sec...
[M::process] read 676476 sequences (100000028 bp) in 1.452 CPU sec, 1.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288352, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.37, 113.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676904 reads in 195.379 CPU sec, 6.289 real sec
sorting block 18, #reads = 1082276 mem = 536877955 peak = 924000256
[M::mem_process_seqs] Processed 676708 reads in 104.704 CPU sec, 3.358 real sec
sorting block 19, #reads = 1082164 mem = 536879963 peak = 946016256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287965, 25, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.65, 115.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 567, 1544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4454)
[M::mem_pestat] mean and std.dev: (618.56, 618.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5909)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676380 sequences (100000158 bp) in 1.995 CPU sec, 2.180 real sec...
[M::process] read 676284 sequences (100000006 bp) in 1.432 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287488, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.78, 117.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676470 reads in 188.817 CPU sec, 6.081 real sec
[M::mem_process_seqs] Processed 676476 reads in 106.104 CPU sec, 3.411 real sec
sorting block 20, #reads = 1082301 mem = 536877130 peak = 946016256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287833, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.25, 116.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 2058, 2058)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5814)
[M::mem_pestat] mean and std.dev: (1258.31, 934.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7692)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676286 sequences (100000025 bp) in 2.087 CPU sec, 2.343 real sec...
[M::process] read 676254 sequences (100000057 bp) in 1.472 CPU sec, 1.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287942, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.98, 118.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676380 reads in 185.183 CPU sec, 6.007 real sec
[M::mem_process_seqs] Processed 676284 reads in 104.600 CPU sec, 3.400 real sec
sorting block 21, #reads = 1081682 mem = 536877673 peak = 946016256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288197, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.49, 120.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 233, 5699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16947)
[M::mem_pestat] mean and std.dev: (1893.85, 2542.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22571)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677514 sequences (100000091 bp) in 1.992 CPU sec, 2.172 real sec...
[M::process] read 677316 sequences (100000023 bp) in 1.531 CPU sec, 1.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288110, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.26, 119.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676286 reads in 184.002 CPU sec, 5.903 real sec
[M::mem_process_seqs] Processed 676254 reads in 103.730 CPU sec, 3.310 real sec
sorting block 22, #reads = 1082207 mem = 536879222 peak = 946016256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288450, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (218.40, 97.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677216 sequences (100000244 bp) in 2.026 CPU sec, 2.293 real sec...
[M::process] read 677102 sequences (100000135 bp) in 1.401 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288167, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (221.25, 100.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677514 reads in 184.990 CPU sec, 5.975 real sec
sorting block 23, #reads = 1082054 mem = 536879022 peak = 961236992
[M::mem_process_seqs] Processed 677316 reads in 105.537 CPU sec, 3.432 real sec
sorting block 24, #reads = 1082434 mem = 536879067 peak = 967073792
[M::process] read 677054 sequences (100000063 bp) in 1.724 CPU sec, 1.869 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288224, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.72, 102.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676624 sequences (100000201 bp) in 1.451 CPU sec, 1.470 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288574, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.69, 104.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288066, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.33, 108.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677216 reads in 272.069 CPU sec, 8.786 real sec
[M::mem_process_seqs] Processed 677102 reads in 190.577 CPU sec, 6.135 real sec
[M::mem_process_seqs] Processed 677054 reads in 199.134 CPU sec, 6.437 real sec
sorting block 25, #reads = 1082612 mem = 536877627 peak = 967073792
[M::process] read 676566 sequences (100000228 bp) in 2.041 CPU sec, 2.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288056, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.29, 110.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 196, 1104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3170)
[M::mem_pestat] mean and std.dev: (505.67, 507.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4203)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 26, #reads = 1082684 mem = 536881530 peak = 967073792
[M::process] read 676534 sequences (100000113 bp) in 2.152 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287762, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.64, 111.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676412 sequences (100000030 bp) in 1.436 CPU sec, 1.491 real sec...
[M::mem_process_seqs] Processed 676624 reads in 190.269 CPU sec, 6.214 real sec
[M::mem_process_seqs] Processed 676566 reads in 107.340 CPU sec, 3.477 real sec
sorting block 27, #reads = 1082038 mem = 536880874 peak = 967073792
[M::process] read 676408 sequences (100000191 bp) in 1.997 CPU sec, 2.343 real sec...
[M::process] read 676182 sequences (100000217 bp) in 1.456 CPU sec, 1.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287793, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.38, 116.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 621, 622)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724)
[M::mem_pestat] mean and std.dev: (495.55, 388.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2275)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676412 reads in 99.741 CPU sec, 3.213 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288138, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.99, 115.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676126 sequences (100000059 bp) in 1.410 CPU sec, 1.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287759, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.63, 116.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676534 reads in 285.883 CPU sec, 9.230 real sec
sorting block 28, #reads = 1081872 mem = 536877332 peak = 967073792
[M::mem_process_seqs] Processed 676408 reads in 115.540 CPU sec, 3.749 real sec
sorting block 29, #reads = 1082030 mem = 536879774 peak = 967073792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287948, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.34, 118.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 21, 990)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2948)
[M::mem_pestat] mean and std.dev: (301.27, 445.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3927)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676112 sequences (100000244 bp) in 2.381 CPU sec, 2.666 real sec...
[M::process] read 677704 sequences (100000012 bp) in 1.398 CPU sec, 1.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288099, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.69, 119.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676182 reads in 186.461 CPU sec, 6.030 real sec
[M::mem_process_seqs] Processed 676126 reads in 104.786 CPU sec, 3.370 real sec
sorting block 30, #reads = 1082005 mem = 536881950 peak = 967073792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287749, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.47, 121.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 16, 1350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4018)
[M::mem_pestat] mean and std.dev: (517.06, 690.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5352)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677632 sequences (100000231 bp) in 2.104 CPU sec, 2.416 real sec...
[M::process] read 677368 sequences (100000158 bp) in 1.455 CPU sec, 1.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288611, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (210.95, 88.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 23, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (120.67, 203.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676112 reads in 186.039 CPU sec, 6.000 real sec
[M::mem_process_seqs] Processed 677704 reads in 104.087 CPU sec, 3.382 real sec
sorting block 31, #reads = 1081756 mem = 536880061 peak = 967073792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289153, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (214.60, 93.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677114 sequences (100000156 bp) in 2.328 CPU sec, 2.680 real sec...
[M::process] read 677078 sequences (100000254 bp) in 1.516 CPU sec, 1.568 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288584, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.91, 101.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 89, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (139.64, 152.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1342)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677632 reads in 186.805 CPU sec, 6.030 real sec
[M::mem_process_seqs] Processed 677368 reads in 105.185 CPU sec, 3.370 real sec
sorting block 32, #reads = 1082298 mem = 536879518 peak = 967073792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288079, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.19, 104.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1082971 mem = 536881287 peak = 1057673216
[M::process] read 676914 sequences (100000096 bp) in 2.084 CPU sec, 2.491 real sec...
[M::process] read 676866 sequences (100000291 bp) in 1.531 CPU sec, 1.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288040, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.40, 107.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677114 reads in 189.543 CPU sec, 6.114 real sec
[M::mem_process_seqs] Processed 677078 reads in 106.829 CPU sec, 3.451 real sec
sorting block 34, #reads = 1082505 mem = 536881542 peak = 1057673216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288464, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (226.68, 107.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676682 sequences (100000132 bp) in 2.208 CPU sec, 2.504 real sec...
[M::process] read 676598 sequences (100000171 bp) in 1.491 CPU sec, 1.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288390, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.53, 109.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 12, 116)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338)
[M::mem_pestat] mean and std.dev: (26.78, 35.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 449)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676914 reads in 188.140 CPU sec, 6.086 real sec
[M::mem_process_seqs] Processed 676866 reads in 106.303 CPU sec, 3.450 real sec
sorting block 35, #reads = 1082354 mem = 536879763 peak = 1057673216
[M::process] read 676658 sequences (100000182 bp) in 2.058 CPU sec, 2.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287859, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.05, 112.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676796 sequences (100000292 bp) in 1.506 CPU sec, 1.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287705, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.33, 112.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287862, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.56, 114.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676682 reads in 271.212 CPU sec, 8.732 real sec
[M::mem_process_seqs] Processed 676598 reads in 191.020 CPU sec, 6.137 real sec
[M::mem_process_seqs] Processed 676658 reads in 199.177 CPU sec, 6.422 real sec
sorting block 36, #reads = 1082792 mem = 536877057 peak = 1057673216
[M::process] read 676672 sequences (100000241 bp) in 2.269 CPU sec, 2.661 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288077, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.42, 114.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 37, #reads = 1082110 mem = 536879221 peak = 1057673216
[M::process] read 677270 sequences (100000177 bp) in 2.072 CPU sec, 2.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287802, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.11, 115.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676796 reads in 190.634 CPU sec, 6.215 real sec
[M::mem_process_seqs] Processed 676672 reads in 110.059 CPU sec, 3.525 real sec
[M::process] read 677902 sequences (100000143 bp) in 1.696 CPU sec, 1.758 real sec...
sorting block 38, #reads = 1082314 mem = 536879587 peak = 1057673216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288776, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.39, 103.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677716 sequences (100000032 bp) in 1.769 CPU sec, 1.914 real sec...
[M::process] read 677422 sequences (100000047 bp) in 1.512 CPU sec, 1.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289356, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488)
[M::mem_pestat] mean and std.dev: (205.41, 81.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 597)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677270 reads in 180.573 CPU sec, 5.811 real sec
[M::mem_process_seqs] Processed 677902 reads in 101.466 CPU sec, 3.263 real sec
sorting block 39, #reads = 1082373 mem = 536877879 peak = 1057673216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 289198, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (214.67, 94.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677178 sequences (100000222 bp) in 1.786 CPU sec, 2.044 real sec...
[M::process] read 677038 sequences (100000189 bp) in 1.509 CPU sec, 1.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288724, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.38, 104.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677716 reads in 180.776 CPU sec, 5.825 real sec
sorting block 40, #reads = 1082891 mem = 536880199 peak = 1057673216
[M::mem_process_seqs] Processed 677422 reads in 102.518 CPU sec, 3.290 real sec
sorting block 41, #reads = 1082743 mem = 536877566 peak = 1099796480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288556, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.29, 106.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676986 sequences (100000131 bp) in 1.980 CPU sec, 2.286 real sec...
[M::process] read 676916 sequences (100000109 bp) in 1.537 CPU sec, 1.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289059, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.13, 107.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 35, 1172)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3466)
[M::mem_pestat] mean and std.dev: (426.00, 677.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4613)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677178 reads in 182.208 CPU sec, 5.888 real sec
[M::mem_process_seqs] Processed 677038 reads in 102.364 CPU sec, 3.310 real sec
sorting block 42, #reads = 1083027 mem = 536879864 peak = 1099796480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288529, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.71, 107.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676986 sequences (100000159 bp) in 1.897 CPU sec, 2.386 real sec...
[M::process] read 676884 sequences (100000151 bp) in 1.484 CPU sec, 1.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288208, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.16, 109.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 187, 964)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2856)
[M::mem_pestat] mean and std.dev: (398.15, 409.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3802)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676986 reads in 185.366 CPU sec, 6.028 real sec
[M::mem_process_seqs] Processed 676916 reads in 106.637 CPU sec, 3.439 real sec
sorting block 43, #reads = 1082779 mem = 536879043 peak = 1099796480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288056, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.03, 110.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676692 sequences (100000231 bp) in 1.996 CPU sec, 2.275 real sec...
[M::process] read 676760 sequences (100000274 bp) in 1.556 CPU sec, 1.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288111, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.89, 112.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676986 reads in 188.087 CPU sec, 6.052 real sec
[M::mem_process_seqs] Processed 676884 reads in 106.944 CPU sec, 3.419 real sec
sorting block 44, #reads = 1081684 mem = 536883847 peak = 1099796480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288353, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.03, 112.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1081827 mem = 536878543 peak = 1129717760
[M::process] read 677116 sequences (100000264 bp) in 1.917 CPU sec, 2.214 real sec...
[M::process] read 678046 sequences (100000125 bp) in 1.619 CPU sec, 1.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288025, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.49, 114.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676692 reads in 185.678 CPU sec, 5.970 real sec
[M::mem_process_seqs] Processed 676760 reads in 105.248 CPU sec, 3.358 real sec
sorting block 46, #reads = 1082306 mem = 536879431 peak = 1129717760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288393, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (222.98, 105.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 35, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1633)
[M::mem_pestat] mean and std.dev: (231.29, 256.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2175)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677610 sequences (100000266 bp) in 2.002 CPU sec, 2.343 real sec...
[M::process] read 677678 sequences (100000016 bp) in 1.554 CPU sec, 1.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289257, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 175, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455)
[M::mem_pestat] mean and std.dev: (200.95, 74.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 553)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677116 reads in 184.807 CPU sec, 5.970 real sec
[M::mem_process_seqs] Processed 678046 reads in 105.425 CPU sec, 3.399 real sec
sorting block 47, #reads = 1082047 mem = 536878605 peak = 1129717760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288410, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (210.38, 88.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677244 sequences (100000224 bp) in 1.995 CPU sec, 2.269 real sec...
[M::process] read 677328 sequences (100000291 bp) in 1.489 CPU sec, 1.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288107, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (218.03, 99.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677610 reads in 186.468 CPU sec, 6.046 real sec
[M::mem_process_seqs] Processed 677678 reads in 105.527 CPU sec, 3.434 real sec
sorting block 48, #reads = 1083190 mem = 536878417 peak = 1129717760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288056, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (222.13, 104.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677126 sequences (100000103 bp) in 1.945 CPU sec, 2.136 real sec...
[M::process] read 677014 sequences (100000278 bp) in 1.554 CPU sec, 1.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288268, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.26, 107.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 38, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1954)
[M::mem_pestat] mean and std.dev: (183.38, 284.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2603)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677244 reads in 185.605 CPU sec, 5.980 real sec
[M::mem_process_seqs] Processed 677328 reads in 104.619 CPU sec, 3.372 real sec
sorting block 49, #reads = 1082665 mem = 536878052 peak = 1129717760
sorting block 50, #reads = 1082444 mem = 536879394 peak = 1129717760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288171, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.27, 107.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 41, 993)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2947)
[M::mem_pestat] mean and std.dev: (340.70, 461.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3924)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676810 sequences (100000028 bp) in 2.029 CPU sec, 2.282 real sec...
[M::process] read 676878 sequences (100000125 bp) in 1.608 CPU sec, 1.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288181, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.44, 109.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677126 reads in 187.063 CPU sec, 6.022 real sec
[M::mem_process_seqs] Processed 677014 reads in 105.652 CPU sec, 3.419 real sec
sorting block 51, #reads = 1082909 mem = 536876942 peak = 1129717760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288153, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (227.68, 110.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676728 sequences (100000171 bp) in 2.281 CPU sec, 2.463 real sec...
[M::process] read 676798 sequences (100000176 bp) in 1.510 CPU sec, 1.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288260, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.08, 110.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676810 reads in 190.265 CPU sec, 6.169 real sec
[M::mem_process_seqs] Processed 676878 reads in 106.375 CPU sec, 3.466 real sec
sorting block 52, #reads = 1082653 mem = 536878776 peak = 1129717760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287888, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.88, 112.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677058 sequences (100000216 bp) in 2.099 CPU sec, 2.243 real sec...
[M::process] read 677968 sequences (100000215 bp) in 1.596 CPU sec, 1.603 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287891, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.10, 112.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 21, 566)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1682)
[M::mem_pestat] mean and std.dev: (179.70, 252.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2240)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676728 reads in 190.738 CPU sec, 6.122 real sec
[M::mem_process_seqs] Processed 676798 reads in 106.955 CPU sec, 3.415 real sec
sorting block 53, #reads = 1082335 mem = 536877269 peak = 1129717760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288065, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (223.67, 106.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677886 sequences (100000012 bp) in 2.259 CPU sec, 2.556 real sec...
[M::process] read 677614 sequences (100000129 bp) in 1.555 CPU sec, 1.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288817, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443)
[M::mem_pestat] mean and std.dev: (200.12, 73.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 537)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677058 reads in 186.762 CPU sec, 6.014 real sec
[M::mem_process_seqs] Processed 677968 reads in 105.597 CPU sec, 3.385 real sec
sorting block 54, #reads = 1082239 mem = 536879471 peak = 1129717760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288564, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515)
[M::mem_pestat] mean and std.dev: (209.74, 87.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 633)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1082484 mem = 536880240 peak = 1129717760
[M::process] read 677536 sequences (100000272 bp) in 2.111 CPU sec, 2.326 real sec...
[M::process] read 677156 sequences (100000066 bp) in 1.319 CPU sec, 1.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288258, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (215.88, 96.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677886 reads in 185.666 CPU sec, 5.982 real sec
[M::mem_process_seqs] Processed 677614 reads in 105.867 CPU sec, 3.388 real sec
sorting block 56, #reads = 1082946 mem = 536880293 peak = 1129717760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288266, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (218.34, 99.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677062 sequences (100000203 bp) in 2.332 CPU sec, 2.521 real sec...
[M::process] read 677088 sequences (100000091 bp) in 1.548 CPU sec, 1.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288037, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (223.01, 105.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677536 reads in 189.599 CPU sec, 6.091 real sec
[M::mem_process_seqs] Processed 677156 reads in 107.579 CPU sec, 3.457 real sec
sorting block 57, #reads = 1082183 mem = 536876983 peak = 1129717760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287922, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.39, 107.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676822 sequences (100000137 bp) in 2.023 CPU sec, 2.400 real sec...
[M::process] read 676892 sequences (100000047 bp) in 1.614 CPU sec, 1.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288051, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.87, 108.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677062 reads in 192.357 CPU sec, 6.206 real sec
[M::mem_process_seqs] Processed 677088 reads in 109.336 CPU sec, 3.505 real sec
sorting block 58, #reads = 1082713 mem = 536879218 peak = 1129717760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287504, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.63, 110.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676710 sequences (100000054 bp) in 2.294 CPU sec, 2.493 real sec...
[M::process] read 676532 sequences (100000065 bp) in 1.517 CPU sec, 1.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288117, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.58, 112.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 1103, 1103)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3193)
[M::mem_pestat] mean and std.dev: (576.92, 526.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4238)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676822 reads in 191.699 CPU sec, 6.157 real sec
[M::mem_process_seqs] Processed 676892 reads in 108.184 CPU sec, 3.466 real sec
sorting block 59, #reads = 1082663 mem = 536877383 peak = 1129717760
sorting block 60, #reads = 1082089 mem = 536882366 peak = 1129717760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288104, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.21, 113.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676656 sequences (100000159 bp) in 2.119 CPU sec, 2.359 real sec...
[M::process] read 33970 sequences (5020993 bp) in 0.270 CPU sec, 0.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287399, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.79, 115.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676710 reads in 189.527 CPU sec, 6.142 real sec
[M::mem_process_seqs] Processed 676532 reads in 105.546 CPU sec, 3.465 real sec
sorting block 61, #reads = 1082375 mem = 536880439 peak = 1129717760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287742, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.31, 115.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 79, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (151.46, 182.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 14404, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (232.42, 115.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676656 reads in 92.022 CPU sec, 3.021 real sec
[M::mem_process_seqs] Processed 33970 reads in 11.417 CPU sec, 0.378 real sec
sorting block 62, #reads = 1082460 mem = 536878414 peak = 1129717760
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67727678
overall: 69744230400 mem 11334.068 user 474.616 sys 397.223 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L008\tPU:L008\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.T21-D0003376_R1.fastq.gz 0003.T21-D0003376_R2.fastq.gz
[main] Real time: 397.264 sec; CPU: 11808.687 sec
sorting block 63, #reads = 63987 mem = 31787929 peak = 1129717760
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2649710649
output reads: 68254527
bam_mem_sort: 64 calls 40.612 user 0.968 sys 42.681 real
bam_write: 64 calls 34.341 user 2.474 sys 70.323 real
execute: 1 calls 19.696 user 1.319 sys 418.934 real
merge_files: 1 calls 19.668 user 1.300 sys 21.582 real
parse_chunk: 1009 calls 45.298 user 12.142 sys 68.170 real
read_chunk: 2718 calls 21.083 user 12.930 sys 323.997 real
sort_block: 1 calls 14.389 user 12.256 sys 314.697 real
write_chunk: 1707 calls 19.259 user 1.128 sys 20.901 real
overall: 2395828224 mem 694.923 user 42.521 sys 418.954 real
ls: cannot access '*.cram': No such file or directory