.command.log - Workdir - DAQ:SENTIEON_BWAMEM (T21-D0003322;L003;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4d/167223bb97bac3302bb2fad11e2abe/.command.log
Size: 342.6 KB
Task: DAQ:SENTIEON_BWAMEM (T21-D0003322;L003;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/01/eb7b0e9eb7ac7207ab02db735923f0/T21-D0003322/T21-D0003322_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4d/167223bb97bac3302bb2fad11e2abe/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/01/eb7b0e9eb7ac7207ab02db735923f0/T21-D0003322/T21-D0003322_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4d/167223bb97bac3302bb2fad11e2abe/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003322.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1856.929 CPU sec, 63.621 real sec
[M::process] read 675406 sequences (100000161 bp) in 1.276 CPU sec, 1.285 real sec...
[M::process] read 675436 sequences (100000220 bp) in 1.940 CPU sec, 1.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286668, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (248.50, 121.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675298 sequences (100000199 bp) in 1.955 CPU sec, 1.963 real sec...
[M::process] read 675294 sequences (100000144 bp) in 1.975 CPU sec, 1.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286104, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.86, 121.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 18, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181)
[M::mem_pestat] mean and std.dev: (34.80, 40.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 237)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675406 reads in 251.403 CPU sec, 7.951 real sec
[M::mem_process_seqs] Processed 675436 reads in 141.294 CPU sec, 4.525 real sec
sorting block 0, #reads = 1080949 mem = 536877296 peak = 625704960
[M::process] read 675278 sequences (100000058 bp) in 2.019 CPU sec, 2.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286305, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (250.18, 123.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675382 sequences (100000238 bp) in 1.811 CPU sec, 1.821 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285933, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.94, 122.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 223, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (160.44, 161.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675298 reads in 250.200 CPU sec, 8.154 real sec
[M::mem_process_seqs] Processed 675294 reads in 141.721 CPU sec, 4.589 real sec
sorting block 1, #reads = 1080625 mem = 536880105 peak = 738496512
[M::process] read 675384 sequences (100000180 bp) in 2.075 CPU sec, 2.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285976, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (249.39, 122.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675454 sequences (100000230 bp) in 1.873 CPU sec, 1.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286958, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.42, 122.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286173, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (246.11, 119.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675278 reads in 366.343 CPU sec, 11.899 real sec
[M::mem_process_seqs] Processed 675382 reads in 255.219 CPU sec, 8.230 real sec
[M::mem_process_seqs] Processed 675384 reads in 266.843 CPU sec, 8.625 real sec
sorting block 2, #reads = 1080752 mem = 536880146 peak = 859860992
[M::process] read 675374 sequences (100000192 bp) in 2.227 CPU sec, 2.651 real sec...
sorting block 3, #reads = 1080837 mem = 536880136 peak = 992530432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286161, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (247.03, 121.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675330 sequences (100000237 bp) in 2.241 CPU sec, 2.423 real sec...
[M::process] read 675320 sequences (100000170 bp) in 1.794 CPU sec, 2.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286506, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.58, 120.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675454 reads in 259.163 CPU sec, 8.494 real sec
[M::mem_process_seqs] Processed 675374 reads in 147.013 CPU sec, 4.784 real sec
sorting block 4, #reads = 1080580 mem = 536882105 peak = 992530432
[M::process] read 675338 sequences (100000077 bp) in 2.149 CPU sec, 2.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285874, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.58, 120.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 95, 99)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253)
[M::mem_pestat] mean and std.dev: (60.33, 41.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 330)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675220 sequences (100000062 bp) in 1.806 CPU sec, 1.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285845, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.47, 121.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675330 reads in 256.393 CPU sec, 8.352 real sec
[M::mem_process_seqs] Processed 675320 reads in 144.618 CPU sec, 4.628 real sec
sorting block 5, #reads = 1081133 mem = 536880618 peak = 1038888960
[M::process] read 675330 sequences (100000211 bp) in 2.158 CPU sec, 2.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286346, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.92, 122.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 39, 857)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2519)
[M::mem_pestat] mean and std.dev: (253.47, 364.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3350)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675222 sequences (100000068 bp) in 1.781 CPU sec, 1.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286037, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (249.33, 122.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285215, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.61, 124.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675338 reads in 380.350 CPU sec, 12.362 real sec
sorting block 6, #reads = 1080909 mem = 536884279 peak = 1050480640
[M::mem_process_seqs] Processed 675220 reads in 264.913 CPU sec, 8.519 real sec
[M::mem_process_seqs] Processed 675330 reads in 276.591 CPU sec, 8.918 real sec
[M::process] read 675142 sequences (100000043 bp) in 2.009 CPU sec, 2.083 real sec...
sorting block 7, #reads = 1080874 mem = 536876590 peak = 1060671488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285667, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.32, 126.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675142 sequences (100000018 bp) in 2.118 CPU sec, 2.734 real sec...
[M::process] read 675128 sequences (100000154 bp) in 1.907 CPU sec, 1.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286081, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (252.03, 125.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675222 reads in 263.783 CPU sec, 8.639 real sec
[M::mem_process_seqs] Processed 675142 reads in 150.609 CPU sec, 4.864 real sec
sorting block 8, #reads = 1080394 mem = 536878116 peak = 1060671488
[M::process] read 675190 sequences (100000023 bp) in 2.174 CPU sec, 2.816 real sec...
sorting block 9, #reads = 1080926 mem = 536876015 peak = 1072726016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285131, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.73, 125.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675110 sequences (100000032 bp) in 1.771 CPU sec, 1.865 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285719, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.90, 125.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285932, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.93, 125.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 211, 799)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2393)
[M::mem_pestat] mean and std.dev: (396.30, 502.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3190)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675142 reads in 384.169 CPU sec, 12.537 real sec
[M::mem_process_seqs] Processed 675128 reads in 268.630 CPU sec, 8.701 real sec
[M::mem_process_seqs] Processed 675190 reads in 279.670 CPU sec, 9.078 real sec
sorting block 10, #reads = 1079884 mem = 536880152 peak = 1072726016
[M::process] read 675020 sequences (100000258 bp) in 2.301 CPU sec, 2.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285671, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.31, 125.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 1952, 1952)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5792)
[M::mem_pestat] mean and std.dev: (986.60, 965.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7712)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675202 sequences (100000218 bp) in 2.293 CPU sec, 2.973 real sec...
[M::process] read 675212 sequences (100000078 bp) in 1.819 CPU sec, 1.821 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285816, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.76, 126.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675110 reads in 265.469 CPU sec, 8.610 real sec
sorting block 11, #reads = 1080438 mem = 536880041 peak = 1083592704
[M::mem_process_seqs] Processed 675020 reads in 148.515 CPU sec, 4.748 real sec
sorting block 12, #reads = 1080692 mem = 536877356 peak = 1099567104
[M::process] read 675264 sequences (100000263 bp) in 2.107 CPU sec, 2.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286141, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.48, 126.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (72, 142, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146)
[M::mem_pestat] mean and std.dev: (134.56, 113.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1504)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675056 sequences (100000122 bp) in 1.839 CPU sec, 2.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285535, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.79, 125.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675202 reads in 260.647 CPU sec, 8.545 real sec
[M::mem_process_seqs] Processed 675212 reads in 148.714 CPU sec, 4.835 real sec
sorting block 13, #reads = 1080718 mem = 536877481 peak = 1099567104
[M::process] read 675186 sequences (100000176 bp) in 2.293 CPU sec, 2.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285654, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.55, 125.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675034 sequences (100000030 bp) in 1.818 CPU sec, 2.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285727, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.53, 125.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 217, 884)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2530)
[M::mem_pestat] mean and std.dev: (243.00, 275.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3353)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285621, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.85, 126.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675264 reads in 381.596 CPU sec, 12.386 real sec
[M::mem_process_seqs] Processed 675056 reads in 265.166 CPU sec, 8.551 real sec
[M::mem_process_seqs] Processed 675186 reads in 276.775 CPU sec, 8.948 real sec
sorting block 14, #reads = 1080534 mem = 536878249 peak = 1099567104
[M::process] read 675042 sequences (100000255 bp) in 2.208 CPU sec, 2.497 real sec...
sorting block 15, #reads = 1080187 mem = 536876802 peak = 1111334912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285438, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.46, 127.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 59, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (123.44, 141.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1421)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674896 sequences (100000096 bp) in 2.195 CPU sec, 2.983 real sec...
[M::process] read 675064 sequences (100000037 bp) in 1.896 CPU sec, 1.930 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285492, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.72, 128.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675034 reads in 268.105 CPU sec, 8.777 real sec
[M::mem_process_seqs] Processed 675042 reads in 149.172 CPU sec, 4.849 real sec
sorting block 16, #reads = 1080233 mem = 536877050 peak = 1111334912
[M::process] read 674998 sequences (100000284 bp) in 2.174 CPU sec, 3.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285809, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (255.37, 128.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674838 sequences (100000273 bp) in 1.807 CPU sec, 1.850 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285061, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.18, 129.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285469, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.58, 130.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674896 reads in 389.758 CPU sec, 12.650 real sec
[M::mem_process_seqs] Processed 675064 reads in 274.242 CPU sec, 8.807 real sec
[M::mem_process_seqs] Processed 674998 reads in 285.707 CPU sec, 9.203 real sec
sorting block 17, #reads = 1080377 mem = 536879554 peak = 1111334912
[M::process] read 675044 sequences (100000218 bp) in 2.223 CPU sec, 2.476 real sec...
sorting block 18, #reads = 1080455 mem = 536878438 peak = 1132048384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284926, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.45, 130.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674682 sequences (100000059 bp) in 2.314 CPU sec, 2.924 real sec...
[M::process] read 674750 sequences (100000008 bp) in 1.832 CPU sec, 1.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285118, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.57, 129.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (576, 1521, 2347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5889)
[M::mem_pestat] mean and std.dev: (1337.33, 918.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7660)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674838 reads in 269.976 CPU sec, 8.844 real sec
sorting block 19, #reads = 1080686 mem = 536880798 peak = 1132048384
[M::process] read 674804 sequences (100000009 bp) in 1.807 CPU sec, 1.968 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285555, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.00, 130.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674682 reads in 139.853 CPU sec, 4.558 real sec
[M::mem_process_seqs] Processed 675044 reads in 334.717 CPU sec, 10.901 real sec
sorting block 20, #reads = 1080418 mem = 536880227 peak = 1132048384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285648, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.78, 130.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674748 sequences (100000242 bp) in 2.504 CPU sec, 3.059 real sec...
[M::process] read 674704 sequences (100000198 bp) in 1.858 CPU sec, 1.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285155, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.55, 132.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674750 reads in 274.113 CPU sec, 8.907 real sec
[M::mem_process_seqs] Processed 674804 reads in 145.892 CPU sec, 4.717 real sec
sorting block 21, #reads = 1080332 mem = 536879324 peak = 1132048384
[M::process] read 674820 sequences (100000058 bp) in 2.101 CPU sec, 2.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285330, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.39, 131.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674660 sequences (100000207 bp) in 1.766 CPU sec, 1.782 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285338, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.60, 133.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285167, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.11, 133.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674748 reads in 387.911 CPU sec, 12.590 real sec
[M::mem_process_seqs] Processed 674820 reads in 271.698 CPU sec, 8.731 real sec
sorting block 22, #reads = 1080056 mem = 536876983 peak = 1143615488
[M::mem_process_seqs] Processed 674704 reads in 283.678 CPU sec, 9.140 real sec
[M::process] read 674602 sequences (100000249 bp) in 2.549 CPU sec, 2.726 real sec...
sorting block 23, #reads = 1079910 mem = 536880016 peak = 1159036928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285367, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.18, 134.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 101, 3561)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10633)
[M::mem_pestat] mean and std.dev: (1113.40, 1602.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14169)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674504 sequences (100000237 bp) in 2.302 CPU sec, 2.722 real sec...
[M::process] read 674666 sequences (100000231 bp) in 1.798 CPU sec, 1.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285004, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.16, 133.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 58, 163)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483)
[M::mem_pestat] mean and std.dev: (45.50, 46.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 643)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674660 reads in 268.066 CPU sec, 8.780 real sec
[M::mem_process_seqs] Processed 674602 reads in 152.694 CPU sec, 4.952 real sec
sorting block 24, #reads = 1079603 mem = 536877058 peak = 1159036928
[M::process] read 674542 sequences (100000039 bp) in 2.166 CPU sec, 3.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285034, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.01, 135.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674396 sequences (100000221 bp) in 1.829 CPU sec, 1.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285364, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.02, 137.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286075, 19, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.04, 137.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 61, 1583)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4627)
[M::mem_pestat] mean and std.dev: (607.32, 835.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6149)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674504 reads in 375.873 CPU sec, 12.209 real sec
[M::mem_process_seqs] Processed 674542 reads in 260.073 CPU sec, 8.356 real sec
sorting block 25, #reads = 1079647 mem = 536880356 peak = 1167089664
[M::mem_process_seqs] Processed 674666 reads in 271.266 CPU sec, 8.746 real sec
sorting block 26, #reads = 1079934 mem = 536879175 peak = 1167089664
[M::process] read 674418 sequences (100000083 bp) in 2.461 CPU sec, 2.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285200, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.91, 137.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674480 sequences (100000066 bp) in 2.086 CPU sec, 2.865 real sec...
[M::process] read 674786 sequences (100000030 bp) in 1.850 CPU sec, 1.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285497, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.71, 138.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 34, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151)
[M::mem_pestat] mean and std.dev: (45.50, 31.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 190)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674396 reads in 256.493 CPU sec, 8.400 real sec
[M::mem_process_seqs] Processed 674418 reads in 144.472 CPU sec, 4.662 real sec
sorting block 27, #reads = 1080024 mem = 536879091 peak = 1167089664
[M::process] read 675322 sequences (100000286 bp) in 2.252 CPU sec, 3.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285135, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (268.29, 139.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 28, #reads = 1080114 mem = 536883275 peak = 1167089664
[M::process] read 675160 sequences (100000131 bp) in 1.841 CPU sec, 2.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285795, 20, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.78, 131.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 362, 2500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7410)
[M::mem_pestat] mean and std.dev: (1296.90, 1717.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9865)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674480 reads in 250.071 CPU sec, 8.188 real sec
[M::mem_process_seqs] Processed 674786 reads in 140.564 CPU sec, 4.569 real sec
sorting block 29, #reads = 1079474 mem = 536881403 peak = 1167089664
[M::process] read 675084 sequences (100000196 bp) in 2.391 CPU sec, 2.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286408, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.42, 125.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 261, 744)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2202)
[M::mem_pestat] mean and std.dev: (297.83, 327.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2931)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675024 sequences (100000088 bp) in 1.843 CPU sec, 2.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286149, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.58, 126.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675322 reads in 243.514 CPU sec, 7.951 real sec
[M::mem_process_seqs] Processed 675160 reads in 137.490 CPU sec, 4.461 real sec
sorting block 30, #reads = 1080921 mem = 536880543 peak = 1167089664
[M::process] read 675112 sequences (100000098 bp) in 2.557 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286671, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (254.08, 126.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675250 sequences (100000073 bp) in 1.820 CPU sec, 1.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285557, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (254.12, 126.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 75, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596)
[M::mem_pestat] mean and std.dev: (81.54, 87.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675084 reads in 243.235 CPU sec, 7.922 real sec
[M::mem_process_seqs] Processed 675024 reads in 141.394 CPU sec, 4.563 real sec
sorting block 31, #reads = 1079938 mem = 536879664 peak = 1167089664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286220, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (253.06, 125.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 14, 101)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289)
[M::mem_pestat] mean and std.dev: (44.46, 45.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 383)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675406 sequences (100000252 bp) in 2.159 CPU sec, 3.008 real sec...
[M::process] read 675392 sequences (100000157 bp) in 1.896 CPU sec, 1.943 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286500, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.31, 123.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675112 reads in 244.607 CPU sec, 7.967 real sec
[M::mem_process_seqs] Processed 675250 reads in 138.592 CPU sec, 4.444 real sec
sorting block 32, #reads = 1080492 mem = 536877837 peak = 1183461376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286328, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.96, 120.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 86, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614)
[M::mem_pestat] mean and std.dev: (71.06, 65.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 33, #reads = 1081171 mem = 536879519 peak = 1183461376
[M::process] read 675384 sequences (100000047 bp) in 2.470 CPU sec, 3.180 real sec...
[M::process] read 675298 sequences (100000117 bp) in 1.822 CPU sec, 1.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286194, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.11, 123.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675406 reads in 247.677 CPU sec, 8.110 real sec
[M::mem_process_seqs] Processed 675392 reads in 140.940 CPU sec, 4.571 real sec
sorting block 34, #reads = 1081147 mem = 536879916 peak = 1183461376
[M::process] read 675140 sequences (100000112 bp) in 2.285 CPU sec, 2.706 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286179, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.60, 124.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (352, 367, 987)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2257)
[M::mem_pestat] mean and std.dev: (575.15, 397.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2892)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675264 sequences (100000282 bp) in 1.825 CPU sec, 1.898 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286034, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.96, 122.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285891, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.81, 124.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675384 reads in 365.286 CPU sec, 11.874 real sec
[M::mem_process_seqs] Processed 675298 reads in 254.786 CPU sec, 8.249 real sec
[M::mem_process_seqs] Processed 675140 reads in 265.839 CPU sec, 8.625 real sec
sorting block 35, #reads = 1081020 mem = 536879123 peak = 1183461376
[M::process] read 675228 sequences (100000242 bp) in 2.566 CPU sec, 2.850 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286233, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.38, 124.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 10, 72)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 210)
[M::mem_pestat] mean and std.dev: (14.88, 21.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 279)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 36, #reads = 1080603 mem = 536879876 peak = 1185202176
[M::process] read 675186 sequences (100000154 bp) in 2.308 CPU sec, 2.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286039, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.31, 124.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675142 sequences (100000172 bp) in 1.856 CPU sec, 2.309 real sec...
[M::mem_process_seqs] Processed 675264 reads in 257.907 CPU sec, 8.453 real sec
[M::process] read 675018 sequences (100000194 bp) in 1.865 CPU sec, 1.906 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286045, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.51, 126.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675186 reads in 132.768 CPU sec, 4.253 real sec
sorting block 37, #reads = 1080676 mem = 536879962 peak = 1185202176
[M::mem_process_seqs] Processed 675228 reads in 331.470 CPU sec, 10.753 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285912, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.12, 126.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 22, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (111.45, 146.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1367)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 38, #reads = 1081097 mem = 536879891 peak = 1186185216
[M::process] read 675228 sequences (100000110 bp) in 2.310 CPU sec, 3.142 real sec...
[M::process] read 675076 sequences (100000023 bp) in 1.889 CPU sec, 2.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285453, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.46, 127.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675142 reads in 269.360 CPU sec, 8.807 real sec
[M::mem_process_seqs] Processed 675018 reads in 148.586 CPU sec, 4.822 real sec
sorting block 39, #reads = 1080528 mem = 536881652 peak = 1186185216
[M::process] read 675094 sequences (100000206 bp) in 2.211 CPU sec, 2.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286026, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.82, 126.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675138 sequences (100000028 bp) in 1.919 CPU sec, 2.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286099, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.57, 127.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 4, 615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1837)
[M::mem_pestat] mean and std.dev: (198.25, 357.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2448)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285715, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.15, 127.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675228 reads in 370.325 CPU sec, 12.024 real sec
[M::mem_process_seqs] Processed 675076 reads in 260.783 CPU sec, 8.413 real sec
[M::mem_process_seqs] Processed 675094 reads in 272.185 CPU sec, 8.809 real sec
sorting block 40, #reads = 1080915 mem = 536877610 peak = 1186185216
[M::process] read 675306 sequences (100000016 bp) in 2.524 CPU sec, 2.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285990, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.30, 126.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1080750 mem = 536879052 peak = 1186185216
[M::process] read 675376 sequences (100000072 bp) in 2.373 CPU sec, 2.688 real sec...
[M::process] read 675226 sequences (100000211 bp) in 1.878 CPU sec, 2.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286398, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.17, 126.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (414, 666, 2753)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7431)
[M::mem_pestat] mean and std.dev: (1037.33, 1015.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9770)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675138 reads in 255.626 CPU sec, 8.387 real sec
[M::mem_process_seqs] Processed 675306 reads in 145.575 CPU sec, 4.732 real sec
sorting block 42, #reads = 1080477 mem = 536879543 peak = 1186185216
[M::process] read 675272 sequences (100000268 bp) in 2.398 CPU sec, 2.850 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286550, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.68, 124.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675202 sequences (100000057 bp) in 1.890 CPU sec, 1.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285543, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.89, 124.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675376 reads in 253.112 CPU sec, 8.237 real sec
[M::mem_process_seqs] Processed 675226 reads in 143.312 CPU sec, 4.602 real sec
sorting block 43, #reads = 1081230 mem = 536879172 peak = 1186185216
[M::process] read 675000 sequences (100000126 bp) in 2.224 CPU sec, 2.975 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285674, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.46, 126.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675032 sequences (100000181 bp) in 1.833 CPU sec, 1.898 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285448, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.36, 126.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 25, 66)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 180)
[M::mem_pestat] mean and std.dev: (21.11, 18.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 237)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675272 reads in 259.620 CPU sec, 8.448 real sec
[M::mem_process_seqs] Processed 675202 reads in 146.760 CPU sec, 4.700 real sec
sorting block 44, #reads = 1080832 mem = 536877333 peak = 1218039808
[M::process] read 675048 sequences (100000065 bp) in 2.220 CPU sec, 2.847 real sec...
sorting block 45, #reads = 1080318 mem = 536877976 peak = 1218347008
[M::process] read 674858 sequences (100000242 bp) in 1.984 CPU sec, 2.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285006, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.76, 127.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 42, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074)
[M::mem_pestat] mean and std.dev: (173.06, 228.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1429)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675032 reads in 137.345 CPU sec, 4.451 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285541, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.81, 128.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674950 sequences (100000235 bp) in 1.855 CPU sec, 1.877 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285768, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (256.05, 129.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 204, 2500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7378)
[M::mem_pestat] mean and std.dev: (784.61, 1097.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9817)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675000 reads in 395.646 CPU sec, 12.863 real sec
[M::mem_process_seqs] Processed 675048 reads in 280.551 CPU sec, 9.051 real sec
sorting block 46, #reads = 1080401 mem = 536880618 peak = 1218347008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285656, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.71, 128.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 10, 26)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 58)
[M::mem_pestat] mean and std.dev: (13.88, 6.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 74)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674968 sequences (100000266 bp) in 2.334 CPU sec, 2.970 real sec...
[M::process] read 674766 sequences (100000129 bp) in 1.869 CPU sec, 1.921 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286029, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.98, 130.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674858 reads in 255.655 CPU sec, 8.325 real sec
sorting block 47, #reads = 1080326 mem = 536882932 peak = 1226055680
[M::mem_process_seqs] Processed 674950 reads in 145.227 CPU sec, 4.662 real sec
sorting block 48, #reads = 1080835 mem = 536879634 peak = 1226055680
[M::process] read 674872 sequences (100000153 bp) in 2.209 CPU sec, 2.868 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285761, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (258.37, 132.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674708 sequences (100000239 bp) in 1.856 CPU sec, 2.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285426, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.62, 131.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674968 reads in 261.830 CPU sec, 8.586 real sec
[M::mem_process_seqs] Processed 674766 reads in 148.288 CPU sec, 4.808 real sec
sorting block 49, #reads = 1080516 mem = 536880907 peak = 1226055680
[M::process] read 674982 sequences (100000282 bp) in 2.343 CPU sec, 2.890 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284924, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.53, 131.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674776 sequences (100000202 bp) in 1.899 CPU sec, 2.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285381, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.22, 131.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286055, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.72, 130.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 32, 563)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1683)
[M::mem_pestat] mean and std.dev: (150.89, 223.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2243)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674872 reads in 380.856 CPU sec, 12.367 real sec
[M::mem_process_seqs] Processed 674708 reads in 264.419 CPU sec, 8.520 real sec
[M::mem_process_seqs] Processed 674982 reads in 275.946 CPU sec, 8.914 real sec
sorting block 50, #reads = 1080519 mem = 536879289 peak = 1226055680
[M::process] read 674712 sequences (100000281 bp) in 2.576 CPU sec, 2.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285618, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 51, #reads = 1080500 mem = 536880277 peak = 1235533824
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (258.81, 132.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 223, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (155.64, 116.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674688 sequences (100000225 bp) in 2.474 CPU sec, 2.760 real sec...
[M::process] read 674742 sequences (100000173 bp) in 1.882 CPU sec, 2.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285528, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.67, 133.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674776 reads in 262.194 CPU sec, 8.571 real sec
[M::mem_process_seqs] Processed 674712 reads in 146.217 CPU sec, 4.750 real sec
sorting block 52, #reads = 1080583 mem = 536879305 peak = 1235533824
[M::process] read 674672 sequences (100000142 bp) in 2.454 CPU sec, 2.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285713, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.50, 135.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674656 sequences (100000284 bp) in 2.008 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286048, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.04, 134.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674688 reads in 254.705 CPU sec, 8.295 real sec
[M::mem_process_seqs] Processed 674742 reads in 143.731 CPU sec, 4.618 real sec
sorting block 53, #reads = 1080540 mem = 536878747 peak = 1235533824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285839, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.52, 136.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 14, 1528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4560)
[M::mem_pestat] mean and std.dev: (207.00, 499.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6076)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674494 sequences (100000128 bp) in 2.159 CPU sec, 2.996 real sec...
[M::process] read 674512 sequences (100000164 bp) in 1.895 CPU sec, 1.960 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285426, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.58, 135.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674672 reads in 253.844 CPU sec, 8.254 real sec
[M::mem_process_seqs] Processed 674656 reads in 143.035 CPU sec, 4.571 real sec
sorting block 54, #reads = 1080300 mem = 536878834 peak = 1252081664
sorting block 55, #reads = 1080018 mem = 536880116 peak = 1252081664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285269, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.07, 137.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674360 sequences (100000243 bp) in 2.204 CPU sec, 2.967 real sec...
[M::process] read 674404 sequences (100000189 bp) in 2.002 CPU sec, 2.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285530, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.28, 137.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674494 reads in 253.252 CPU sec, 8.296 real sec
[M::mem_process_seqs] Processed 674512 reads in 141.491 CPU sec, 4.589 real sec
sorting block 56, #reads = 1080528 mem = 536879566 peak = 1252081664
[M::process] read 674470 sequences (100000000 bp) in 2.410 CPU sec, 2.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285425, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.93, 137.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674730 sequences (100000146 bp) in 1.871 CPU sec, 1.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285445, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.69, 138.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 25, 1583)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4731)
[M::mem_pestat] mean and std.dev: (630.00, 717.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6305)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674360 reads in 248.803 CPU sec, 8.093 real sec
[M::mem_process_seqs] Processed 674404 reads in 140.721 CPU sec, 4.534 real sec
sorting block 57, #reads = 1079955 mem = 536879307 peak = 1252081664
[M::process] read 675152 sequences (100000010 bp) in 2.329 CPU sec, 2.870 real sec...
[M::process] read 675232 sequences (100000200 bp) in 1.872 CPU sec, 1.950 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286519, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.56, 133.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674730 reads in 124.995 CPU sec, 3.996 real sec
sorting block 58, #reads = 1079339 mem = 536879344 peak = 1252081664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285603, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.20, 139.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (305, 420, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (75, 650)
[M::mem_pestat] mean and std.dev: (372.50, 96.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675176 sequences (100000120 bp) in 1.860 CPU sec, 1.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286581, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (252.00, 124.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674470 reads in 366.561 CPU sec, 11.960 real sec
[M::mem_process_seqs] Processed 675152 reads in 149.262 CPU sec, 4.886 real sec
sorting block 59, #reads = 1079662 mem = 536877554 peak = 1252081664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286101, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.47, 126.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674982 sequences (100000110 bp) in 2.532 CPU sec, 3.034 real sec...
[M::process] read 674954 sequences (100000243 bp) in 1.891 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285928, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (254.30, 127.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675232 reads in 238.164 CPU sec, 7.754 real sec
[M::mem_process_seqs] Processed 675176 reads in 135.850 CPU sec, 4.396 real sec
sorting block 60, #reads = 1080215 mem = 536880126 peak = 1252081664
[M::process] read 674880 sequences (100000158 bp) in 2.234 CPU sec, 2.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285890, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.73, 127.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 58, 109)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243)
[M::mem_pestat] mean and std.dev: (83.00, 78.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 397)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675296 sequences (100000198 bp) in 1.787 CPU sec, 1.791 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286164, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.60, 127.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 23, 76)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198)
[M::mem_pestat] mean and std.dev: (28.69, 28.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 259)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674982 reads in 238.050 CPU sec, 7.763 real sec
[M::mem_process_seqs] Processed 674954 reads in 134.093 CPU sec, 4.302 real sec
sorting block 61, #reads = 1080605 mem = 536879997 peak = 1260511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285930, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.00, 129.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2753, 4026, 4026)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (207, 6572)
[M::mem_pestat] mean and std.dev: (3866.88, 421.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7845)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 62, #reads = 1079886 mem = 536877511 peak = 1260511232
[M::process] read 675426 sequences (100000100 bp) in 2.380 CPU sec, 3.049 real sec...
[M::process] read 675380 sequences (100000028 bp) in 1.955 CPU sec, 2.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286148, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.95, 125.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 202, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212)
[M::mem_pestat] mean and std.dev: (228.27, 182.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1611)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674880 reads in 246.394 CPU sec, 8.084 real sec
[M::mem_process_seqs] Processed 675296 reads in 138.700 CPU sec, 4.497 real sec
sorting block 63, #reads = 1080501 mem = 536875453 peak = 1260511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286394, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.19, 121.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675364 sequences (100000273 bp) in 2.233 CPU sec, 2.844 real sec...
[M::process] read 675226 sequences (100000126 bp) in 1.918 CPU sec, 2.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286498, 26, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.81, 124.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 42, 66)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 170)
[M::mem_pestat] mean and std.dev: (31.45, 23.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 222)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675426 reads in 240.072 CPU sec, 7.826 real sec
[M::mem_process_seqs] Processed 675380 reads in 136.772 CPU sec, 4.422 real sec
sorting block 64, #reads = 1080914 mem = 536880943 peak = 1260511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285771, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.26, 124.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675250 sequences (100000223 bp) in 2.326 CPU sec, 3.038 real sec...
[M::process] read 675298 sequences (100000004 bp) in 1.917 CPU sec, 1.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286353, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.76, 124.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675364 reads in 244.317 CPU sec, 7.957 real sec
[M::mem_process_seqs] Processed 675226 reads in 137.360 CPU sec, 4.419 real sec
sorting block 65, #reads = 1080687 mem = 536879630 peak = 1260511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286377, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 675098 sequences (100000102 bp) in 2.423 CPU sec, 3.066 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.71, 124.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (37, 88, 730)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2116)
[M::mem_pestat] mean and std.dev: (329.80, 349.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2809)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675126 sequences (100000090 bp) in 1.841 CPU sec, 1.856 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286739, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.86, 125.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675250 reads in 242.847 CPU sec, 7.916 real sec
[M::mem_process_seqs] Processed 675298 reads in 134.952 CPU sec, 4.317 real sec
sorting block 66, #reads = 1080661 mem = 536879998 peak = 1260511232
sorting block 67, #reads = 1081098 mem = 536879484 peak = 1260511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285911, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.07, 128.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674968 sequences (100000133 bp) in 2.338 CPU sec, 3.000 real sec...
[M::process] read 675218 sequences (100000214 bp) in 1.880 CPU sec, 2.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285730, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.54, 127.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 58, 58)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 140)
[M::mem_pestat] mean and std.dev: (40.54, 22.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 181)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675098 reads in 253.640 CPU sec, 8.310 real sec
[M::mem_process_seqs] Processed 675126 reads in 143.154 CPU sec, 4.653 real sec
sorting block 68, #reads = 1080604 mem = 536874640 peak = 1260511232
[M::process] read 674988 sequences (100000023 bp) in 2.328 CPU sec, 2.763 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285962, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.81, 128.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 26, 1108)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3274)
[M::mem_pestat] mean and std.dev: (437.14, 535.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4357)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675062 sequences (100000094 bp) in 1.867 CPU sec, 1.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285608, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.82, 127.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 2, 7)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19)
[M::mem_pestat] mean and std.dev: (2.00, 1.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 25)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674968 reads in 253.134 CPU sec, 8.264 real sec
[M::mem_process_seqs] Processed 675218 reads in 145.341 CPU sec, 4.714 real sec
sorting block 69, #reads = 1080194 mem = 536879388 peak = 1260511232
[M::process] read 675198 sequences (100000283 bp) in 2.291 CPU sec, 3.119 real sec...
[M::process] read 675222 sequences (100000260 bp) in 1.910 CPU sec, 1.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285929, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.77, 128.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675062 reads in 134.084 CPU sec, 4.290 real sec
sorting block 70, #reads = 1080717 mem = 536879159 peak = 1260511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286009, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.91, 130.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675380 sequences (100000247 bp) in 1.878 CPU sec, 2.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285397, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.16, 127.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 379, 942)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2490)
[M::mem_pestat] mean and std.dev: (421.15, 367.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3264)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674988 reads in 382.213 CPU sec, 12.456 real sec
[M::mem_process_seqs] Processed 675198 reads in 154.430 CPU sec, 5.049 real sec
sorting block 71, #reads = 1080831 mem = 536877403 peak = 1260511232
[M::process] read 675166 sequences (100000085 bp) in 2.769 CPU sec, 2.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285984, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.27, 126.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675048 sequences (100000075 bp) in 1.904 CPU sec, 1.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286165, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.16, 123.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675222 reads in 249.916 CPU sec, 8.137 real sec
[M::mem_process_seqs] Processed 675380 reads in 139.600 CPU sec, 4.496 real sec
sorting block 72, #reads = 1080118 mem = 536879504 peak = 1260511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286110, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.91, 124.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 17, 38)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 80)
[M::mem_pestat] mean and std.dev: (18.78, 13.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 101)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675104 sequences (100000184 bp) in 2.197 CPU sec, 3.088 real sec...
[M::process] read 675016 sequences (100000213 bp) in 1.980 CPU sec, 2.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286166, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.48, 126.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 367, 1503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4219)
[M::mem_pestat] mean and std.dev: (557.67, 684.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5577)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675166 reads in 248.547 CPU sec, 8.079 real sec
[M::mem_process_seqs] Processed 675048 reads in 141.345 CPU sec, 4.505 real sec
sorting block 73, #reads = 1081201 mem = 536879377 peak = 1268789248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286262, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 74, #reads = 1081071 mem = 536879754 peak = 1268789248
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.00, 128.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675046 sequences (100000225 bp) in 2.625 CPU sec, 3.404 real sec...
[M::process] read 674958 sequences (100000273 bp) in 1.964 CPU sec, 2.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285595, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (254.04, 128.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675104 reads in 259.253 CPU sec, 8.501 real sec
[M::mem_process_seqs] Processed 675016 reads in 145.724 CPU sec, 4.729 real sec
sorting block 75, #reads = 1080436 mem = 536878944 peak = 1268789248
[M::process] read 674906 sequences (100000111 bp) in 2.313 CPU sec, 2.624 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286103, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.57, 129.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674902 sequences (100000006 bp) in 1.873 CPU sec, 1.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285905, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.91, 130.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285453, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.41, 128.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675046 reads in 375.018 CPU sec, 12.202 real sec
[M::mem_process_seqs] Processed 674958 reads in 262.904 CPU sec, 8.502 real sec
[M::mem_process_seqs] Processed 674906 reads in 274.163 CPU sec, 8.886 real sec
sorting block 76, #reads = 1080360 mem = 536880394 peak = 1268789248
[M::process] read 675014 sequences (100000251 bp) in 2.741 CPU sec, 3.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285919, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.49, 130.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 77, #reads = 1081061 mem = 536879330 peak = 1270231040
[M::process] read 674772 sequences (100000100 bp) in 2.485 CPU sec, 2.729 real sec...
[M::process] read 674796 sequences (100000149 bp) in 1.872 CPU sec, 2.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285634, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (258.10, 131.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 15, 81)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213)
[M::mem_pestat] mean and std.dev: (32.11, 40.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 279)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674902 reads in 262.630 CPU sec, 8.605 real sec
[M::mem_process_seqs] Processed 675014 reads in 148.718 CPU sec, 4.844 real sec
sorting block 78, #reads = 1080755 mem = 536877684 peak = 1270231040
[M::process] read 674792 sequences (100000077 bp) in 2.353 CPU sec, 3.129 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285148, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.49, 131.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674766 sequences (100000071 bp) in 1.997 CPU sec, 2.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285655, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.72, 131.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 42, 577)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1681)
[M::mem_pestat] mean and std.dev: (192.27, 241.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2233)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285487, 14, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.63, 131.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 202, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (156.00, 141.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674772 reads in 381.075 CPU sec, 12.358 real sec
[M::mem_process_seqs] Processed 674796 reads in 265.502 CPU sec, 8.553 real sec
[M::mem_process_seqs] Processed 674792 reads in 276.918 CPU sec, 8.948 real sec
sorting block 79, #reads = 1079687 mem = 536878858 peak = 1270231040
[M::process] read 674824 sequences (100000069 bp) in 2.312 CPU sec, 2.585 real sec...
sorting block 80, #reads = 1080896 mem = 536877357 peak = 1279598592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285377, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.59, 131.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674486 sequences (100000222 bp) in 2.388 CPU sec, 3.107 real sec...
[M::process] read 674794 sequences (100000266 bp) in 1.898 CPU sec, 1.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285143, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.55, 132.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 176, 7494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22418)
[M::mem_pestat] mean and std.dev: (2593.33, 3468.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 29880)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674766 reads in 262.144 CPU sec, 8.580 real sec
[M::mem_process_seqs] Processed 674824 reads in 149.576 CPU sec, 4.871 real sec
sorting block 81, #reads = 1080300 mem = 536879189 peak = 1279598592
[M::process] read 674600 sequences (100000078 bp) in 2.308 CPU sec, 2.682 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285363, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.82, 134.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674518 sequences (100000029 bp) in 1.848 CPU sec, 1.907 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285746, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.20, 133.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674486 reads in 256.129 CPU sec, 8.323 real sec
[M::mem_process_seqs] Processed 674794 reads in 143.471 CPU sec, 4.600 real sec
sorting block 82, #reads = 1080266 mem = 536879006 peak = 1279598592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285262, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.09, 135.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674498 sequences (100000110 bp) in 2.251 CPU sec, 2.973 real sec...
[M::process] read 674630 sequences (100000151 bp) in 1.869 CPU sec, 1.876 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285353, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.98, 135.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674600 reads in 251.379 CPU sec, 8.175 real sec
[M::mem_process_seqs] Processed 674518 reads in 143.835 CPU sec, 4.598 real sec
sorting block 83, #reads = 1080151 mem = 536878311 peak = 1279598592
[M::process] read 674664 sequences (100000203 bp) in 2.289 CPU sec, 2.672 real sec...
sorting block 84, #reads = 1079623 mem = 536879336 peak = 1284710400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284921, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.02, 135.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674612 sequences (100000145 bp) in 1.855 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285343, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.23, 136.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285704, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.62, 136.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 271, 1007)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3013)
[M::mem_pestat] mean and std.dev: (249.73, 355.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4016)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674498 reads in 374.208 CPU sec, 12.211 real sec
[M::mem_process_seqs] Processed 674664 reads in 259.949 CPU sec, 8.410 real sec
[M::mem_process_seqs] Processed 674630 reads in 271.469 CPU sec, 8.804 real sec
sorting block 85, #reads = 1079656 mem = 536878893 peak = 1284710400
[M::process] read 674648 sequences (100000124 bp) in 2.608 CPU sec, 2.726 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285242, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.44, 136.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 1864, 3366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9656)
[M::mem_pestat] mean and std.dev: (1724.36, 1412.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12801)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 86, #reads = 1080321 mem = 536878255 peak = 1286209536
[M::process] read 674414 sequences (100000095 bp) in 2.451 CPU sec, 2.792 real sec...
[M::process] read 674698 sequences (100000186 bp) in 1.994 CPU sec, 1.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285668, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.14, 138.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674612 reads in 255.158 CPU sec, 8.341 real sec
[M::mem_process_seqs] Processed 674648 reads in 144.120 CPU sec, 4.696 real sec
sorting block 87, #reads = 1080044 mem = 536883239 peak = 1286209536
[M::process] read 675108 sequences (100000273 bp) in 2.490 CPU sec, 2.794 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285278, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.46, 137.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 17, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (73.45, 100.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675192 sequences (100000199 bp) in 1.870 CPU sec, 1.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286482, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.31, 134.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 61, 126)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352)
[M::mem_pestat] mean and std.dev: (59.09, 43.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 465)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286324, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.67, 124.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674414 reads in 352.689 CPU sec, 11.457 real sec
[M::mem_process_seqs] Processed 674698 reads in 245.627 CPU sec, 7.942 real sec
[M::mem_process_seqs] Processed 675108 reads in 256.639 CPU sec, 8.314 real sec
sorting block 88, #reads = 1079229 mem = 536880603 peak = 1286209536
[M::process] read 675072 sequences (100000268 bp) in 2.482 CPU sec, 2.687 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286541, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (252.09, 125.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 19, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 189)
[M::mem_pestat] mean and std.dev: (22.75, 19.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 247)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 89, #reads = 1079886 mem = 536878388 peak = 1286209536
[M::process] read 675136 sequences (100000103 bp) in 2.376 CPU sec, 3.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285755, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.95, 124.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675006 sequences (100000015 bp) in 1.935 CPU sec, 2.008 real sec...
[M::mem_process_seqs] Processed 675192 reads in 247.112 CPU sec, 8.102 real sec
[M::mem_process_seqs] Processed 675072 reads in 142.017 CPU sec, 4.626 real sec
sorting block 90, #reads = 1080500 mem = 536883687 peak = 1286209536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286794, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.59, 126.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674938 sequences (100000193 bp) in 2.381 CPU sec, 2.923 real sec...
[M::process] read 674944 sequences (100000086 bp) in 1.999 CPU sec, 2.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286008, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.86, 126.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675136 reads in 242.021 CPU sec, 7.867 real sec
[M::mem_process_seqs] Processed 675006 reads in 137.768 CPU sec, 4.433 real sec
sorting block 91, #reads = 1080669 mem = 536877712 peak = 1286209536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286180, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (254.93, 127.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675290 sequences (100000018 bp) in 2.301 CPU sec, 2.859 real sec...
[M::process] read 675370 sequences (100000186 bp) in 1.856 CPU sec, 1.893 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285943, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.36, 127.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 21, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120)
[M::mem_pestat] mean and std.dev: (175.50, 206.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1489)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674938 reads in 243.952 CPU sec, 7.946 real sec
[M::mem_process_seqs] Processed 674944 reads in 140.323 CPU sec, 4.496 real sec
sorting block 92, #reads = 1080031 mem = 536880545 peak = 1286209536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286416, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.76, 123.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675374 sequences (100000258 bp) in 2.297 CPU sec, 3.012 real sec...
[M::process] read 675156 sequences (100000129 bp) in 1.979 CPU sec, 1.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286521, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.66, 122.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675290 reads in 243.277 CPU sec, 7.915 real sec
sorting block 93, #reads = 1080118 mem = 536880836 peak = 1286209536
[M::mem_process_seqs] Processed 675370 reads in 138.406 CPU sec, 4.418 real sec
sorting block 94, #reads = 1080678 mem = 536880973 peak = 1286238208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286486, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.45, 123.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675378 sequences (100000162 bp) in 2.369 CPU sec, 2.916 real sec...
[M::process] read 675184 sequences (100000017 bp) in 1.887 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286589, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.30, 124.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675374 reads in 240.556 CPU sec, 7.892 real sec
[M::mem_process_seqs] Processed 675156 reads in 136.467 CPU sec, 4.446 real sec
sorting block 95, #reads = 1080236 mem = 536876982 peak = 1286238208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286575, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.36, 124.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675040 sequences (100000286 bp) in 2.346 CPU sec, 2.919 real sec...
[M::process] read 675160 sequences (100000238 bp) in 1.973 CPU sec, 1.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286431, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.27, 125.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 100, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (158.33, 179.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675378 reads in 244.686 CPU sec, 7.971 real sec
[M::mem_process_seqs] Processed 675184 reads in 138.249 CPU sec, 4.460 real sec
sorting block 96, #reads = 1081139 mem = 536876336 peak = 1286238208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286006, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.59, 126.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675226 sequences (100000139 bp) in 2.292 CPU sec, 3.164 real sec...
[M::process] read 675176 sequences (100000050 bp) in 1.966 CPU sec, 1.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286375, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.45, 127.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675040 reads in 242.243 CPU sec, 7.885 real sec
[M::mem_process_seqs] Processed 675160 reads in 136.479 CPU sec, 4.365 real sec
sorting block 97, #reads = 1080669 mem = 536879244 peak = 1286238208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285987, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.77, 124.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674984 sequences (100000129 bp) in 2.262 CPU sec, 3.062 real sec...
sorting block 98, #reads = 1080744 mem = 536876298 peak = 1294340096
[M::process] read 675030 sequences (100000208 bp) in 2.002 CPU sec, 2.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286370, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.48, 125.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675226 reads in 245.008 CPU sec, 8.035 real sec
[M::mem_process_seqs] Processed 675176 reads in 138.365 CPU sec, 4.477 real sec
sorting block 99, #reads = 1080090 mem = 536879146 peak = 1294340096
[M::process] read 675164 sequences (100000045 bp) in 2.366 CPU sec, 2.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285533, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.61, 128.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 480, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640)
[M::mem_pestat] mean and std.dev: (375.83, 408.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2178)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675088 sequences (100000199 bp) in 1.910 CPU sec, 1.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285748, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.04, 128.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 53, 174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516)
[M::mem_pestat] mean and std.dev: (97.14, 106.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 687)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286303, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.77, 127.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674984 reads in 365.896 CPU sec, 11.880 real sec
[M::mem_process_seqs] Processed 675030 reads in 255.233 CPU sec, 8.245 real sec
[M::mem_process_seqs] Processed 675164 reads in 266.556 CPU sec, 8.634 real sec
sorting block 100, #reads = 1080932 mem = 536879460 peak = 1294340096
[M::process] read 675174 sequences (100000092 bp) in 2.421 CPU sec, 2.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286137, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.79, 127.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 101, #reads = 1080637 mem = 536880108 peak = 1294340096
[M::process] read 675052 sequences (100000100 bp) in 2.474 CPU sec, 2.725 real sec...
[M::process] read 675176 sequences (100000015 bp) in 1.867 CPU sec, 2.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285920, 17, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.55, 125.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 20, 1450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4334)
[M::mem_pestat] mean and std.dev: (558.12, 796.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5776)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675088 reads in 253.529 CPU sec, 8.306 real sec
[M::mem_process_seqs] Processed 675174 reads in 144.152 CPU sec, 4.693 real sec
sorting block 102, #reads = 1080464 mem = 536880078 peak = 1294340096
[M::process] read 674996 sequences (100000234 bp) in 2.462 CPU sec, 2.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286000, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.26, 125.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674984 sequences (100000110 bp) in 1.913 CPU sec, 2.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286036, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.49, 126.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675052 reads in 252.981 CPU sec, 8.224 real sec
[M::mem_process_seqs] Processed 675176 reads in 142.793 CPU sec, 4.595 real sec
sorting block 103, #reads = 1080909 mem = 536878391 peak = 1294340096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286015, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (253.24, 127.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675072 sequences (100000275 bp) in 2.398 CPU sec, 2.963 real sec...
[M::process] read 675100 sequences (100000106 bp) in 1.900 CPU sec, 1.904 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285896, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.48, 127.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674996 reads in 250.059 CPU sec, 8.146 real sec
[M::mem_process_seqs] Processed 674984 reads in 141.198 CPU sec, 4.521 real sec
sorting block 104, #reads = 1080744 mem = 536878904 peak = 1294340096
[M::process] read 674876 sequences (100000060 bp) in 2.353 CPU sec, 2.894 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285897, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.55, 127.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675026 sequences (100000145 bp) in 1.893 CPU sec, 1.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286002, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.81, 127.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675072 reads in 255.704 CPU sec, 8.301 real sec
sorting block 105, #reads = 1080721 mem = 536879607 peak = 1295736832
[M::mem_process_seqs] Processed 675100 reads in 143.594 CPU sec, 4.586 real sec
sorting block 106, #reads = 1080485 mem = 536878623 peak = 1295736832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285809, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674918 sequences (100000064 bp) in 2.549 CPU sec, 3.165 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.44, 128.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674950 sequences (100000248 bp) in 1.854 CPU sec, 2.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285719, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.96, 128.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 121, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358)
[M::mem_pestat] mean and std.dev: (56.88, 52.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674876 reads in 254.912 CPU sec, 8.356 real sec
[M::mem_process_seqs] Processed 675026 reads in 142.412 CPU sec, 4.630 real sec
sorting block 107, #reads = 1080640 mem = 536879333 peak = 1295736832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286000, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.46, 128.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 362, 598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1764)
[M::mem_pestat] mean and std.dev: (264.07, 248.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2347)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674846 sequences (100000141 bp) in 2.366 CPU sec, 2.914 real sec...
[M::process] read 674814 sequences (100000287 bp) in 1.915 CPU sec, 2.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285492, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.42, 128.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674918 reads in 250.777 CPU sec, 8.165 real sec
[M::mem_process_seqs] Processed 674950 reads in 141.705 CPU sec, 4.565 real sec
sorting block 108, #reads = 1080486 mem = 536880037 peak = 1295736832
[M::process] read 674900 sequences (100000218 bp) in 2.510 CPU sec, 3.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285578, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.68, 129.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 19, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (69.71, 90.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674716 sequences (100000086 bp) in 1.908 CPU sec, 1.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285955, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.71, 130.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674846 reads in 254.979 CPU sec, 8.298 real sec
[M::mem_process_seqs] Processed 674814 reads in 141.824 CPU sec, 4.536 real sec
sorting block 109, #reads = 1080868 mem = 536881508 peak = 1296748544
[M::process] read 674770 sequences (100000051 bp) in 2.315 CPU sec, 3.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285631, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.37, 130.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674770 sequences (100000245 bp) in 1.886 CPU sec, 1.907 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285860, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.15, 130.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674900 reads in 253.416 CPU sec, 8.223 real sec
sorting block 110, #reads = 1080172 mem = 536878840 peak = 1296855040
[M::mem_process_seqs] Processed 674716 reads in 140.471 CPU sec, 4.485 real sec
sorting block 111, #reads = 1080613 mem = 536878563 peak = 1296896000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285629, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.19, 131.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 66, 2623)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7843)
[M::mem_pestat] mean and std.dev: (880.19, 1477.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10453)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674618 sequences (100000234 bp) in 2.509 CPU sec, 3.086 real sec...
[M::process] read 674606 sequences (100000136 bp) in 1.899 CPU sec, 2.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286201, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.73, 131.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674770 reads in 249.015 CPU sec, 8.157 real sec
[M::mem_process_seqs] Processed 674770 reads in 140.967 CPU sec, 4.573 real sec
sorting block 112, #reads = 1080275 mem = 536879900 peak = 1296896000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285237, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.08, 132.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 83, 183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465)
[M::mem_pestat] mean and std.dev: (91.31, 65.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 606)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674590 sequences (100000099 bp) in 2.192 CPU sec, 3.028 real sec...
[M::process] read 674534 sequences (100000247 bp) in 1.930 CPU sec, 2.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285913, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.55, 133.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 15, 16)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 40)
[M::mem_pestat] mean and std.dev: (8.67, 6.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 52)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674618 reads in 252.306 CPU sec, 8.237 real sec
[M::mem_process_seqs] Processed 674606 reads in 141.627 CPU sec, 4.577 real sec
sorting block 113, #reads = 1079831 mem = 536879821 peak = 1296896000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285537, 11, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.19, 133.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 236, 2031)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5897)
[M::mem_pestat] mean and std.dev: (666.91, 897.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7830)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674596 sequences (100000023 bp) in 2.585 CPU sec, 3.275 real sec...
[M::process] read 674518 sequences (100000095 bp) in 1.897 CPU sec, 1.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285432, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.37, 134.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674590 reads in 251.268 CPU sec, 8.164 real sec
[M::mem_process_seqs] Processed 674534 reads in 142.421 CPU sec, 4.562 real sec
sorting block 114, #reads = 1080257 mem = 536882023 peak = 1296896000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285925, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.49, 134.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 480, 987)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2613)
[M::mem_pestat] mean and std.dev: (487.50, 361.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3426)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 115, #reads = 1079976 mem = 536878655 peak = 1301524480
[M::process] read 674444 sequences (100000064 bp) in 2.337 CPU sec, 3.132 real sec...
[M::process] read 674434 sequences (100000047 bp) in 1.996 CPU sec, 2.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286251, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.94, 135.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (379, 1741, 4492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12718)
[M::mem_pestat] mean and std.dev: (2224.00, 1939.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16831)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674596 reads in 245.123 CPU sec, 8.046 real sec
[M::mem_process_seqs] Processed 674518 reads in 140.516 CPU sec, 4.566 real sec
sorting block 116, #reads = 1079886 mem = 536877516 peak = 1301524480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285885, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.38, 135.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674406 sequences (100000254 bp) in 2.657 CPU sec, 3.183 real sec...
[M::process] read 675296 sequences (100000048 bp) in 2.014 CPU sec, 2.023 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285510, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.19, 135.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674444 reads in 247.536 CPU sec, 8.060 real sec
[M::mem_process_seqs] Processed 674434 reads in 140.381 CPU sec, 4.549 real sec
sorting block 117, #reads = 1080483 mem = 536879340 peak = 1301524480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285868, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.50, 137.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 87, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097)
[M::mem_pestat] mean and std.dev: (154.70, 162.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1462)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675700 sequences (100000045 bp) in 2.461 CPU sec, 2.865 real sec...
[M::process] read 675758 sequences (100000213 bp) in 1.888 CPU sec, 1.897 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286491, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.60, 129.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674406 reads in 247.551 CPU sec, 8.070 real sec
[M::mem_process_seqs] Processed 675296 reads in 142.575 CPU sec, 4.591 real sec
sorting block 118, #reads = 1080064 mem = 536878619 peak = 1301524480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285711, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (245.24, 123.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675444 sequences (100000043 bp) in 2.498 CPU sec, 3.334 real sec...
[M::process] read 675408 sequences (100000116 bp) in 1.894 CPU sec, 1.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286221, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (247.08, 126.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (427, 427, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (207, 757)
[M::mem_pestat] mean and std.dev: (501.89, 76.51)
[M::mem_pestat] low and high boundaries for proper pairs: (97, 867)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675700 reads in 246.388 CPU sec, 8.009 real sec
[M::mem_process_seqs] Processed 675758 reads in 132.606 CPU sec, 4.243 real sec
sorting block 119, #reads = 1080132 mem = 536881087 peak = 1308565504
sorting block 120, #reads = 1079407 mem = 536877524 peak = 1308930048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286399, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.24, 125.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675534 sequences (100000137 bp) in 2.452 CPU sec, 2.934 real sec...
[M::process] read 675634 sequences (100000132 bp) in 1.941 CPU sec, 2.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285581, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.44, 124.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 882, 1811)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5301)
[M::mem_pestat] mean and std.dev: (925.73, 840.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7046)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675444 reads in 259.143 CPU sec, 8.498 real sec
[M::mem_process_seqs] Processed 675408 reads in 147.861 CPU sec, 4.798 real sec
sorting block 121, #reads = 1080422 mem = 536880055 peak = 1308930048
[M::process] read 675658 sequences (100000110 bp) in 2.716 CPU sec, 3.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285119, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (246.99, 124.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675490 sequences (100000175 bp) in 2.016 CPU sec, 2.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285167, 22, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (246.07, 123.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 88, 1690)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5064)
[M::mem_pestat] mean and std.dev: (661.50, 813.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6751)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285733, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.61, 123.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675534 reads in 379.925 CPU sec, 12.334 real sec
[M::mem_process_seqs] Processed 675634 reads in 264.253 CPU sec, 8.539 real sec
[M::mem_process_seqs] Processed 675658 reads in 275.970 CPU sec, 8.939 real sec
sorting block 122, #reads = 1079968 mem = 536880081 peak = 1308930048
[M::process] read 675710 sequences (100000135 bp) in 2.605 CPU sec, 2.900 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285578, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.23, 124.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 22, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123)
[M::mem_pestat] mean and std.dev: (144.67, 170.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1490)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 123, #reads = 1080532 mem = 536878652 peak = 1308930048
[M::process] read 675510 sequences (100000206 bp) in 2.574 CPU sec, 2.945 real sec...
[M::process] read 675602 sequences (100000063 bp) in 2.039 CPU sec, 2.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285276, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.60, 125.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675490 reads in 268.356 CPU sec, 8.783 real sec
[M::mem_process_seqs] Processed 675710 reads in 151.556 CPU sec, 4.938 real sec
sorting block 124, #reads = 1080320 mem = 536878719 peak = 1308930048
[M::process] read 675376 sequences (100000072 bp) in 2.423 CPU sec, 3.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285590, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.17, 125.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675428 sequences (100000154 bp) in 1.963 CPU sec, 2.019 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285855, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.51, 126.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675510 reads in 257.218 CPU sec, 8.358 real sec
[M::mem_process_seqs] Processed 675602 reads in 141.665 CPU sec, 4.546 real sec
sorting block 125, #reads = 1079913 mem = 536878951 peak = 1308930048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284986, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.82, 127.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 178, 1121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3329)
[M::mem_pestat] mean and std.dev: (377.25, 454.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4433)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675438 sequences (100000198 bp) in 2.317 CPU sec, 3.225 real sec...
[M::process] read 675448 sequences (100000223 bp) in 1.980 CPU sec, 2.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285632, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.46, 126.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675376 reads in 264.217 CPU sec, 8.592 real sec
[M::mem_process_seqs] Processed 675428 reads in 149.702 CPU sec, 4.783 real sec
sorting block 126, #reads = 1079074 mem = 536880376 peak = 1308930048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284940, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.73, 126.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675290 sequences (100000261 bp) in 2.449 CPU sec, 3.030 real sec...
sorting block 127, #reads = 1077210 mem = 536876405 peak = 1310093312
[M::process] read 675268 sequences (100000049 bp) in 1.975 CPU sec, 2.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285562, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.46, 125.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675438 reads in 234.677 CPU sec, 7.696 real sec
[M::mem_process_seqs] Processed 675448 reads in 138.387 CPU sec, 4.495 real sec
sorting block 128, #reads = 1074864 mem = 536876458 peak = 1310093312
[M::process] read 675328 sequences (100000116 bp) in 2.395 CPU sec, 3.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285522, 23, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.08, 126.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 1471, 1955)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5507)
[M::mem_pestat] mean and std.dev: (1104.04, 764.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7283)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675152 sequences (100000258 bp) in 2.045 CPU sec, 2.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285712, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.52, 127.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285692, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.28, 126.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675290 reads in 381.312 CPU sec, 12.381 real sec
[M::mem_process_seqs] Processed 675328 reads in 256.159 CPU sec, 8.279 real sec
[M::mem_process_seqs] Processed 675268 reads in 268.376 CPU sec, 8.694 real sec
sorting block 129, #reads = 1078261 mem = 536880089 peak = 1310093312
[M::process] read 675250 sequences (100000011 bp) in 2.688 CPU sec, 2.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285610, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.31, 127.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 130, #reads = 1078578 mem = 536880096 peak = 1310093312
[M::process] read 675150 sequences (100000088 bp) in 2.441 CPU sec, 3.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285202, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.38, 125.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675098 sequences (100000281 bp) in 1.999 CPU sec, 2.112 real sec...
[M::mem_process_seqs] Processed 675152 reads in 263.724 CPU sec, 8.652 real sec
[M::mem_process_seqs] Processed 675250 reads in 146.898 CPU sec, 4.791 real sec
sorting block 131, #reads = 1080212 mem = 536878224 peak = 1310093312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286033, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.87, 123.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675208 sequences (100000120 bp) in 2.705 CPU sec, 3.405 real sec...
[M::process] read 675244 sequences (100000297 bp) in 1.934 CPU sec, 2.078 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285769, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.55, 123.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 212, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (141.17, 163.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675150 reads in 260.943 CPU sec, 8.489 real sec
[M::mem_process_seqs] Processed 675098 reads in 153.391 CPU sec, 4.947 real sec
sorting block 132, #reads = 1079136 mem = 536878952 peak = 1310093312
[M::process] read 675152 sequences (100000060 bp) in 2.347 CPU sec, 2.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285165, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.77, 123.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675054 sequences (100000219 bp) in 1.950 CPU sec, 1.968 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284818, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.86, 124.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 341, 875)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2557)
[M::mem_pestat] mean and std.dev: (353.25, 327.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3398)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675208 reads in 273.588 CPU sec, 8.891 real sec
[M::mem_process_seqs] Processed 675244 reads in 156.355 CPU sec, 4.993 real sec
sorting block 133, #reads = 1079080 mem = 536878305 peak = 1310093312
[M::process] read 674988 sequences (100000279 bp) in 2.628 CPU sec, 3.322 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284534, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.88, 128.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675084 sequences (100000214 bp) in 2.074 CPU sec, 2.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284798, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.07, 129.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284493, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.81, 127.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675152 reads in 403.922 CPU sec, 13.078 real sec
sorting block 134, #reads = 1080141 mem = 536879737 peak = 1310470144
[M::mem_process_seqs] Processed 674988 reads in 279.421 CPU sec, 8.969 real sec
[M::mem_process_seqs] Processed 675054 reads in 291.279 CPU sec, 9.376 real sec
sorting block 135, #reads = 1080201 mem = 536878852 peak = 1310470144
[M::process] read 675144 sequences (100000215 bp) in 2.665 CPU sec, 2.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284509, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.70, 128.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675110 sequences (100000136 bp) in 2.475 CPU sec, 3.023 real sec...
[M::process] read 675092 sequences (100000085 bp) in 1.977 CPU sec, 1.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284447, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.70, 129.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675084 reads in 285.907 CPU sec, 9.281 real sec
[M::mem_process_seqs] Processed 675144 reads in 157.814 CPU sec, 5.058 real sec
sorting block 136, #reads = 1080170 mem = 536881815 peak = 1310470144
sorting block 137, #reads = 1080563 mem = 536879821 peak = 1310470144
[M::process] read 675010 sequences (100000012 bp) in 2.564 CPU sec, 3.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284653, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.92, 129.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675004 sequences (100000002 bp) in 1.910 CPU sec, 2.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284892, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.06, 129.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 58, 1607)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4785)
[M::mem_pestat] mean and std.dev: (590.50, 717.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6374)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675110 reads in 281.050 CPU sec, 9.186 real sec
[M::mem_process_seqs] Processed 675092 reads in 155.352 CPU sec, 5.040 real sec
sorting block 138, #reads = 1080345 mem = 536879747 peak = 1310470144
[M::process] read 675110 sequences (100000276 bp) in 2.707 CPU sec, 2.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284657, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.04, 130.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675086 sequences (100000213 bp) in 1.986 CPU sec, 2.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284704, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.18, 131.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284639, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.01, 127.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675010 reads in 412.705 CPU sec, 13.409 real sec
[M::mem_process_seqs] Processed 675004 reads in 286.578 CPU sec, 9.240 real sec
[M::mem_process_seqs] Processed 675110 reads in 298.307 CPU sec, 9.641 real sec
sorting block 139, #reads = 1080423 mem = 536880699 peak = 1310470144
[M::process] read 674852 sequences (100000180 bp) in 2.525 CPU sec, 2.845 real sec...
sorting block 140, #reads = 1080172 mem = 536881959 peak = 1310470144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284314, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.86, 130.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 16, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (70.67, 98.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674906 sequences (100000021 bp) in 2.472 CPU sec, 2.830 real sec...
[M::process] read 675018 sequences (100000158 bp) in 2.061 CPU sec, 2.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284386, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.92, 133.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675086 reads in 284.686 CPU sec, 9.303 real sec
[M::mem_process_seqs] Processed 674852 reads in 158.471 CPU sec, 5.151 real sec
sorting block 141, #reads = 1079997 mem = 536880799 peak = 1310470144
[M::process] read 674798 sequences (100000110 bp) in 2.529 CPU sec, 2.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284420, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (258.51, 134.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674884 sequences (100000249 bp) in 1.973 CPU sec, 2.019 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285241, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.53, 135.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285003, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.28, 134.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (72, 221, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098)
[M::mem_pestat] mean and std.dev: (218.36, 172.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1440)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674906 reads in 407.304 CPU sec, 13.206 real sec
[M::mem_process_seqs] Processed 675018 reads in 280.365 CPU sec, 9.012 real sec
[M::mem_process_seqs] Processed 674798 reads in 292.139 CPU sec, 9.411 real sec
sorting block 142, #reads = 1080274 mem = 536877170 peak = 1310470144
[M::process] read 674886 sequences (100000161 bp) in 2.462 CPU sec, 2.806 real sec...
sorting block 143, #reads = 1080805 mem = 536881265 peak = 1310470144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284170, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.73, 134.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674812 sequences (100000297 bp) in 2.613 CPU sec, 2.648 real sec...
[M::process] read 674834 sequences (100000020 bp) in 1.975 CPU sec, 2.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284262, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (260.10, 135.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674884 reads in 285.345 CPU sec, 9.295 real sec
[M::mem_process_seqs] Processed 674886 reads in 157.460 CPU sec, 5.093 real sec
sorting block 144, #reads = 1080591 mem = 536879592 peak = 1310470144
[M::process] read 674758 sequences (100000146 bp) in 2.420 CPU sec, 3.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285410, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (260.57, 135.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674796 sequences (100000261 bp) in 1.938 CPU sec, 1.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285351, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (260.01, 135.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674812 reads in 270.113 CPU sec, 8.774 real sec
[M::mem_process_seqs] Processed 674834 reads in 151.007 CPU sec, 4.831 real sec
sorting block 145, #reads = 1079876 mem = 536878561 peak = 1310470144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284687, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (261.47, 136.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 500, 5722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17136)
[M::mem_pestat] mean and std.dev: (2512.42, 2718.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22843)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675084 sequences (100000187 bp) in 2.700 CPU sec, 3.367 real sec...
[M::process] read 675514 sequences (100000097 bp) in 1.971 CPU sec, 1.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285022, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (262.00, 139.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (37, 256, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (181.69, 124.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674758 reads in 272.379 CPU sec, 8.833 real sec
sorting block 146, #reads = 1080605 mem = 536877671 peak = 1311612928
[M::mem_process_seqs] Processed 674796 reads in 153.068 CPU sec, 4.900 real sec
sorting block 147, #reads = 1079884 mem = 536880279 peak = 1311612928
[M::process] read 675378 sequences (100000129 bp) in 2.431 CPU sec, 3.024 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285076, 27, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.33, 132.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (104, 331, 770)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2102)
[M::mem_pestat] mean and std.dev: (402.85, 357.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2768)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675454 sequences (100000123 bp) in 2.028 CPU sec, 2.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285680, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.46, 126.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285853, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.71, 127.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675084 reads in 377.230 CPU sec, 12.286 real sec
[M::mem_process_seqs] Processed 675514 reads in 259.800 CPU sec, 8.406 real sec
[M::mem_process_seqs] Processed 675378 reads in 271.171 CPU sec, 8.792 real sec
sorting block 148, #reads = 1080181 mem = 536878480 peak = 1311612928
[M::process] read 675334 sequences (100000199 bp) in 2.834 CPU sec, 3.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285580, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.18, 126.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 61, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 139)
[M::mem_pestat] mean and std.dev: (38.79, 21.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 178)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 149, #reads = 1080404 mem = 536878591 peak = 1311612928
[M::process] read 675506 sequences (100000096 bp) in 2.626 CPU sec, 3.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286057, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.61, 124.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675454 reads in 254.664 CPU sec, 8.336 real sec
[M::process] read 675550 sequences (100000090 bp) in 2.030 CPU sec, 2.137 real sec...
[M::mem_process_seqs] Processed 675334 reads in 144.747 CPU sec, 4.711 real sec
sorting block 150, #reads = 1080260 mem = 536877976 peak = 1311612928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286229, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (248.44, 125.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 15, 72)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 194)
[M::mem_pestat] mean and std.dev: (21.12, 20.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 255)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675506 sequences (100000225 bp) in 2.450 CPU sec, 3.340 real sec...
[M::process] read 675670 sequences (100000064 bp) in 2.050 CPU sec, 2.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285885, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.79, 120.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 103, 179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513)
[M::mem_pestat] mean and std.dev: (109.70, 109.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 680)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675506 reads in 251.999 CPU sec, 8.207 real sec
[M::mem_process_seqs] Processed 675550 reads in 150.534 CPU sec, 4.874 real sec
sorting block 151, #reads = 1079399 mem = 536879692 peak = 1311612928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286302, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (245.65, 120.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 25, 25)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 47)
[M::mem_pestat] mean and std.dev: (17.93, 8.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 58)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675534 sequences (100000050 bp) in 2.348 CPU sec, 3.237 real sec...
[M::process] read 675486 sequences (100000269 bp) in 2.028 CPU sec, 2.037 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285706, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.92, 120.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675506 reads in 247.844 CPU sec, 8.058 real sec
[M::mem_process_seqs] Processed 675670 reads in 140.914 CPU sec, 4.496 real sec
sorting block 152, #reads = 1079637 mem = 536878192 peak = 1311612928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286140, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (247.24, 123.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 73, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 175)
[M::mem_pestat] mean and std.dev: (45.33, 28.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 226)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675444 sequences (100000254 bp) in 2.406 CPU sec, 3.156 real sec...
[M::process] read 675586 sequences (100000254 bp) in 2.084 CPU sec, 2.088 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286205, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.62, 124.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 23, 39)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 107)
[M::mem_pestat] mean and std.dev: (13.88, 12.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 141)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675534 reads in 251.242 CPU sec, 8.150 real sec
sorting block 153, #reads = 1080920 mem = 536879230 peak = 1311612928
[M::mem_process_seqs] Processed 675486 reads in 141.810 CPU sec, 4.520 real sec
sorting block 154, #reads = 1080855 mem = 536881440 peak = 1312010240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285610, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.14, 124.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 72, 88)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 224)
[M::mem_pestat] mean and std.dev: (44.57, 31.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 292)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675466 sequences (100000246 bp) in 2.539 CPU sec, 3.097 real sec...
[M::process] read 675264 sequences (100000054 bp) in 1.970 CPU sec, 2.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286408, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.41, 126.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 16, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199)
[M::mem_pestat] mean and std.dev: (14.00, 16.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 264)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675444 reads in 239.216 CPU sec, 7.851 real sec
[M::mem_process_seqs] Processed 675586 reads in 134.777 CPU sec, 4.406 real sec
sorting block 155, #reads = 1079604 mem = 536879649 peak = 1312010240
[M::process] read 675454 sequences (100000260 bp) in 2.549 CPU sec, 2.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286037, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (250.93, 126.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675278 sequences (100000249 bp) in 1.953 CPU sec, 2.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285977, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.46, 127.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 210, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614)
[M::mem_pestat] mean and std.dev: (103.22, 98.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675466 reads in 249.138 CPU sec, 8.134 real sec
[M::mem_process_seqs] Processed 675264 reads in 132.545 CPU sec, 4.284 real sec
sorting block 156, #reads = 1074505 mem = 536876941 peak = 1312010240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285961, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.65, 126.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 23, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (50.91, 78.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675316 sequences (100000056 bp) in 2.402 CPU sec, 3.285 real sec...
[M::process] read 675284 sequences (100000093 bp) in 1.975 CPU sec, 2.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285448, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.25, 126.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675454 reads in 253.685 CPU sec, 8.253 real sec
[M::mem_process_seqs] Processed 675278 reads in 154.324 CPU sec, 4.953 real sec
sorting block 157, #reads = 1074353 mem = 536880133 peak = 1312010240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286325, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.03, 126.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675176 sequences (100000249 bp) in 2.444 CPU sec, 3.169 real sec...
[M::process] read 675312 sequences (100000240 bp) in 2.103 CPU sec, 2.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286282, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.07, 127.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 397, 737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2147)
[M::mem_pestat] mean and std.dev: (337.10, 315.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2852)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675316 reads in 258.344 CPU sec, 8.375 real sec
sorting block 158, #reads = 1072849 mem = 536880875 peak = 1312374784
[M::mem_process_seqs] Processed 675284 reads in 149.759 CPU sec, 4.777 real sec
sorting block 159, #reads = 1077224 mem = 536879354 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285489, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.71, 127.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675312 sequences (100000041 bp) in 2.520 CPU sec, 3.083 real sec...
[M::process] read 675264 sequences (100000168 bp) in 2.063 CPU sec, 2.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286057, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.28, 128.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 41, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (42.09, 58.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675176 reads in 254.933 CPU sec, 8.368 real sec
[M::mem_process_seqs] Processed 675312 reads in 141.224 CPU sec, 4.588 real sec
sorting block 160, #reads = 1077879 mem = 536876710 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286371, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.79, 125.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675218 sequences (100000094 bp) in 2.564 CPU sec, 3.276 real sec...
[M::process] read 675364 sequences (100000285 bp) in 2.067 CPU sec, 2.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286416, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.68, 122.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 14, 14)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 14)
[M::mem_pestat] mean and std.dev: (14.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (14, 14)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675312 reads in 242.301 CPU sec, 7.885 real sec
[M::mem_process_seqs] Processed 675264 reads in 130.401 CPU sec, 4.195 real sec
sorting block 161, #reads = 1081095 mem = 536876175 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285832, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.24, 123.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675208 sequences (100000236 bp) in 2.469 CPU sec, 3.133 real sec...
[M::process] read 675380 sequences (100000076 bp) in 1.990 CPU sec, 2.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286222, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.47, 123.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675218 reads in 245.302 CPU sec, 7.977 real sec
[M::mem_process_seqs] Processed 675364 reads in 144.203 CPU sec, 4.615 real sec
sorting block 162, #reads = 1077681 mem = 536878366 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285940, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.30, 127.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675198 sequences (100000199 bp) in 2.537 CPU sec, 3.101 real sec...
sorting block 163, #reads = 1078161 mem = 536878395 peak = 1313894400
[M::process] read 675266 sequences (100000261 bp) in 2.012 CPU sec, 2.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284996, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.51, 128.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675208 reads in 269.572 CPU sec, 8.822 real sec
[M::mem_process_seqs] Processed 675380 reads in 153.084 CPU sec, 4.962 real sec
sorting block 164, #reads = 1080785 mem = 536877914 peak = 1313894400
[M::process] read 675074 sequences (100000137 bp) in 2.579 CPU sec, 3.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285034, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.86, 129.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675100 sequences (100000282 bp) in 2.055 CPU sec, 2.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285296, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.11, 127.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 453, 857)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2493)
[M::mem_pestat] mean and std.dev: (394.94, 354.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3311)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285621, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.02, 128.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 90, 574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1700)
[M::mem_pestat] mean and std.dev: (200.42, 233.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2263)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675198 reads in 359.977 CPU sec, 11.696 real sec
[M::mem_process_seqs] Processed 675266 reads in 241.910 CPU sec, 7.809 real sec
[M::mem_process_seqs] Processed 675074 reads in 253.683 CPU sec, 8.210 real sec
sorting block 165, #reads = 1080050 mem = 536875905 peak = 1313894400
[M::process] read 675170 sequences (100000219 bp) in 2.644 CPU sec, 3.037 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285026, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.71, 129.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 22, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161)
[M::mem_pestat] mean and std.dev: (40.89, 46.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 229)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 166, #reads = 1078450 mem = 536877784 peak = 1313894400
[M::process] read 675142 sequences (100000192 bp) in 2.592 CPU sec, 2.874 real sec...
[M::process] read 675038 sequences (100000105 bp) in 2.065 CPU sec, 2.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284966, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.10, 128.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675100 reads in 275.158 CPU sec, 9.000 real sec
[M::mem_process_seqs] Processed 675170 reads in 153.058 CPU sec, 4.974 real sec
sorting block 167, #reads = 1080026 mem = 536880478 peak = 1313894400
[M::process] read 675126 sequences (100000145 bp) in 2.691 CPU sec, 3.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285261, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.00, 130.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675182 sequences (100000118 bp) in 1.988 CPU sec, 2.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284839, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.29, 130.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675142 reads in 275.448 CPU sec, 8.953 real sec
[M::mem_process_seqs] Processed 675038 reads in 151.010 CPU sec, 4.852 real sec
sorting block 168, #reads = 1079545 mem = 536879240 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285045, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.19, 129.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674940 sequences (100000157 bp) in 2.649 CPU sec, 3.539 real sec...
[M::process] read 674930 sequences (100000178 bp) in 1.995 CPU sec, 2.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285616, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.13, 127.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675126 reads in 276.002 CPU sec, 8.969 real sec
[M::mem_process_seqs] Processed 675182 reads in 159.959 CPU sec, 5.108 real sec
sorting block 169, #reads = 1079138 mem = 536879390 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284618, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (254.99, 130.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 215, 1215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3623)
[M::mem_pestat] mean and std.dev: (438.00, 547.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4827)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674922 sequences (100000027 bp) in 2.497 CPU sec, 3.121 real sec...
[M::process] read 675090 sequences (100000246 bp) in 2.024 CPU sec, 2.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284955, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.25, 132.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 53, 737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2167)
[M::mem_pestat] mean and std.dev: (286.58, 433.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2882)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674940 reads in 268.102 CPU sec, 8.711 real sec
sorting block 170, #reads = 1077505 mem = 536878387 peak = 1313894400
[M::process] read 675020 sequences (100000278 bp) in 2.345 CPU sec, 2.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284833, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.57, 131.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 23, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1646)
[M::mem_pestat] mean and std.dev: (216.00, 263.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2187)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674922 reads in 145.110 CPU sec, 4.732 real sec
sorting block 171, #reads = 1079333 mem = 536880321 peak = 1313894400
[M::mem_process_seqs] Processed 674930 reads in 355.312 CPU sec, 11.507 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285169, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.75, 134.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 53, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (82.92, 120.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1381)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675034 sequences (100000094 bp) in 2.393 CPU sec, 3.318 real sec...
[M::process] read 675110 sequences (100000091 bp) in 2.102 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285393, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.50, 134.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675090 reads in 276.731 CPU sec, 8.950 real sec
[M::mem_process_seqs] Processed 675020 reads in 154.771 CPU sec, 4.939 real sec
sorting block 172, #reads = 1080293 mem = 536878583 peak = 1313894400
sorting block 173, #reads = 1080127 mem = 536877957 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284792, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.50, 134.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 563, 3325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9495)
[M::mem_pestat] mean and std.dev: (1449.54, 1494.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12580)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675042 sequences (100000088 bp) in 2.557 CPU sec, 3.209 real sec...
[M::process] read 675014 sequences (100000251 bp) in 2.154 CPU sec, 2.593 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285570, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (256.58, 134.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675034 reads in 250.004 CPU sec, 8.190 real sec
[M::mem_process_seqs] Processed 675110 reads in 131.759 CPU sec, 4.276 real sec
sorting block 174, #reads = 1079251 mem = 536880564 peak = 1313894400
[M::process] read 674998 sequences (100000179 bp) in 2.549 CPU sec, 3.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284633, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.57, 134.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675226 sequences (100000201 bp) in 2.049 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285940, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.53, 134.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 447, 3667)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10915)
[M::mem_pestat] mean and std.dev: (1632.76, 1708.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14539)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675042 reads in 277.865 CPU sec, 9.021 real sec
[M::mem_process_seqs] Processed 675014 reads in 155.919 CPU sec, 5.022 real sec
sorting block 175, #reads = 1075568 mem = 536877011 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285326, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.91, 134.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675306 sequences (100000228 bp) in 2.353 CPU sec, 3.315 real sec...
[M::process] read 675242 sequences (100000176 bp) in 2.096 CPU sec, 2.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285592, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (255.52, 132.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 32, 132)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (44.00, 51.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 510)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674998 reads in 249.140 CPU sec, 8.096 real sec
[M::mem_process_seqs] Processed 675226 reads in 141.649 CPU sec, 4.527 real sec
sorting block 176, #reads = 1076042 mem = 536878721 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285228, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.12, 127.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 223, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1626)
[M::mem_pestat] mean and std.dev: (218.58, 207.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2164)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675138 sequences (100000015 bp) in 2.371 CPU sec, 3.183 real sec...
[M::process] read 675326 sequences (100000209 bp) in 2.048 CPU sec, 2.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285010, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.95, 127.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 20, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133)
[M::mem_pestat] mean and std.dev: (222.09, 367.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1694)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675306 reads in 258.524 CPU sec, 8.408 real sec
sorting block 177, #reads = 1078394 mem = 536877648 peak = 1313894400
[M::mem_process_seqs] Processed 675242 reads in 146.404 CPU sec, 4.671 real sec
sorting block 178, #reads = 1079563 mem = 536879126 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285360, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.04, 126.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675188 sequences (100000236 bp) in 2.538 CPU sec, 3.017 real sec...
[M::process] read 675068 sequences (100000204 bp) in 1.990 CPU sec, 2.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285720, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.88, 125.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675138 reads in 258.435 CPU sec, 8.458 real sec
[M::mem_process_seqs] Processed 675326 reads in 144.095 CPU sec, 4.676 real sec
sorting block 179, #reads = 1080370 mem = 536879281 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285220, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.20, 128.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675492 sequences (100000031 bp) in 2.421 CPU sec, 3.146 real sec...
[M::process] read 675644 sequences (100000215 bp) in 2.001 CPU sec, 2.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285389, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.13, 129.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675188 reads in 262.501 CPU sec, 8.546 real sec
[M::mem_process_seqs] Processed 675068 reads in 146.537 CPU sec, 4.720 real sec
sorting block 180, #reads = 1080272 mem = 536880371 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285716, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.73, 123.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 13, 174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (52.40, 77.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675484 sequences (100000262 bp) in 2.540 CPU sec, 3.153 real sec...
[M::process] read 675294 sequences (100000149 bp) in 2.013 CPU sec, 2.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286097, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (246.99, 122.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675492 reads in 255.524 CPU sec, 8.305 real sec
[M::mem_process_seqs] Processed 675644 reads in 143.503 CPU sec, 4.581 real sec
sorting block 181, #reads = 1080254 mem = 536876724 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286206, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.41, 121.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675266 sequences (100000125 bp) in 2.547 CPU sec, 3.140 real sec...
[M::process] read 675298 sequences (100000105 bp) in 2.013 CPU sec, 2.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285435, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.34, 123.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 663, 3706)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11028)
[M::mem_pestat] mean and std.dev: (1521.16, 1687.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14689)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675484 reads in 248.404 CPU sec, 8.069 real sec
sorting block 182, #reads = 1080586 mem = 536877143 peak = 1313894400
[M::mem_process_seqs] Processed 675294 reads in 145.645 CPU sec, 4.653 real sec
sorting block 183, #reads = 1080479 mem = 536879343 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286113, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.16, 124.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 90, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276)
[M::mem_pestat] mean and std.dev: (62.42, 54.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 362)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675438 sequences (100000229 bp) in 2.640 CPU sec, 3.174 real sec...
[M::process] read 675358 sequences (100000002 bp) in 2.162 CPU sec, 2.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285992, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.11, 124.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675266 reads in 250.421 CPU sec, 8.201 real sec
[M::mem_process_seqs] Processed 675298 reads in 139.865 CPU sec, 4.539 real sec
sorting block 184, #reads = 1080218 mem = 536877050 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285738, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.72, 126.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675254 sequences (100000073 bp) in 2.616 CPU sec, 2.974 real sec...
[M::process] read 675270 sequences (100000192 bp) in 2.022 CPU sec, 2.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285493, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.92, 127.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 53, 158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404)
[M::mem_pestat] mean and std.dev: (55.50, 42.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 527)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675438 reads in 242.050 CPU sec, 7.882 real sec
[M::mem_process_seqs] Processed 675358 reads in 132.600 CPU sec, 4.279 real sec
sorting block 185, #reads = 1079596 mem = 536876981 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285233, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.74, 127.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 87, 654)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1940)
[M::mem_pestat] mean and std.dev: (399.25, 524.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2583)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675280 sequences (100000166 bp) in 2.545 CPU sec, 3.209 real sec...
[M::process] read 675092 sequences (100000135 bp) in 2.006 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285112, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.19, 127.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675254 reads in 248.905 CPU sec, 8.105 real sec
[M::mem_process_seqs] Processed 675270 reads in 146.032 CPU sec, 4.683 real sec
sorting block 186, #reads = 1076137 mem = 536880431 peak = 1313894400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285544, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.20, 128.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 187, #reads = 1069347 mem = 536879418 peak = 1314074624
[M::process] read 675248 sequences (100000206 bp) in 2.544 CPU sec, 3.502 real sec...
[M::process] read 675268 sequences (100000272 bp) in 2.119 CPU sec, 2.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284845, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.54, 128.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 58, 606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1792)
[M::mem_pestat] mean and std.dev: (250.25, 291.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2385)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675280 reads in 244.036 CPU sec, 7.996 real sec
[M::mem_process_seqs] Processed 675092 reads in 133.670 CPU sec, 4.342 real sec
sorting block 188, #reads = 1078157 mem = 536887638 peak = 1314074624
[M::process] read 675312 sequences (100000043 bp) in 2.723 CPU sec, 2.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285495, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.06, 128.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675266 sequences (100000049 bp) in 2.060 CPU sec, 2.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285112, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.88, 127.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 58, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096)
[M::mem_pestat] mean and std.dev: (124.60, 141.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1442)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285386, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.56, 125.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (72, 229, 642)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1782)
[M::mem_pestat] mean and std.dev: (258.08, 264.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2352)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675248 reads in 342.412 CPU sec, 11.120 real sec
[M::mem_process_seqs] Processed 675268 reads in 223.546 CPU sec, 7.242 real sec
[M::mem_process_seqs] Processed 675312 reads in 236.186 CPU sec, 7.674 real sec
sorting block 189, #reads = 1078544 mem = 536877996 peak = 1314074624
[M::process] read 675336 sequences (100000154 bp) in 2.826 CPU sec, 3.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285438, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (246.90, 122.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 190, #reads = 1076469 mem = 536879977 peak = 1314074624
[M::process] read 675456 sequences (100000014 bp) in 2.682 CPU sec, 3.083 real sec...
[M::process] read 675266 sequences (100000260 bp) in 2.090 CPU sec, 2.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285367, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.30, 123.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675266 reads in 266.315 CPU sec, 8.719 real sec
[M::mem_process_seqs] Processed 675336 reads in 150.159 CPU sec, 4.884 real sec
sorting block 191, #reads = 1079719 mem = 536878490 peak = 1314074624
[M::process] read 675342 sequences (100000276 bp) in 2.652 CPU sec, 2.861 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285247, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (247.85, 124.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 362, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1577)
[M::mem_pestat] mean and std.dev: (312.20, 207.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2093)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675322 sequences (100000065 bp) in 2.045 CPU sec, 2.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285511, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.75, 126.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675456 reads in 268.542 CPU sec, 8.732 real sec
[M::mem_process_seqs] Processed 675266 reads in 152.571 CPU sec, 4.921 real sec
sorting block 192, #reads = 1080210 mem = 536877431 peak = 1314074624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284683, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.77, 126.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675112 sequences (100000069 bp) in 2.488 CPU sec, 3.306 real sec...
[M::process] read 675228 sequences (100000078 bp) in 2.051 CPU sec, 2.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285176, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.77, 126.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675342 reads in 260.433 CPU sec, 8.456 real sec
[M::mem_process_seqs] Processed 675322 reads in 149.992 CPU sec, 4.794 real sec
sorting block 193, #reads = 1080837 mem = 536878101 peak = 1314074624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284899, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.58, 128.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 126, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1658)
[M::mem_pestat] mean and std.dev: (276.07, 403.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2205)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675088 sequences (100000042 bp) in 2.761 CPU sec, 3.477 real sec...
[M::process] read 675090 sequences (100000286 bp) in 2.027 CPU sec, 2.056 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284834, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.11, 129.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 448, 652)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1936)
[M::mem_pestat] mean and std.dev: (359.25, 398.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2578)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675112 reads in 274.996 CPU sec, 8.914 real sec
sorting block 194, #reads = 1079486 mem = 536877757 peak = 1314074624
[M::mem_process_seqs] Processed 675228 reads in 152.438 CPU sec, 4.879 real sec
sorting block 195, #reads = 1080441 mem = 536878609 peak = 1314074624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284877, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.28, 129.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675148 sequences (100000215 bp) in 2.554 CPU sec, 3.234 real sec...
[M::process] read 675162 sequences (100000210 bp) in 2.047 CPU sec, 2.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285131, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.72, 128.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675088 reads in 276.463 CPU sec, 9.052 real sec
[M::mem_process_seqs] Processed 675090 reads in 153.685 CPU sec, 4.990 real sec
sorting block 196, #reads = 1080711 mem = 536878816 peak = 1314074624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285429, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.42, 130.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675066 sequences (100000136 bp) in 2.787 CPU sec, 3.489 real sec...
[M::process] read 675100 sequences (100000224 bp) in 2.053 CPU sec, 2.295 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284435, 17, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.67, 129.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 50, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1994)
[M::mem_pestat] mean and std.dev: (252.88, 312.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2654)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675148 reads in 276.805 CPU sec, 8.991 real sec
[M::mem_process_seqs] Processed 675162 reads in 155.835 CPU sec, 5.004 real sec
sorting block 197, #reads = 1081034 mem = 536878559 peak = 1314074624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285053, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.30, 129.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 101, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (97.45, 79.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675104 sequences (100000076 bp) in 2.467 CPU sec, 3.505 real sec...
[M::process] read 674944 sequences (100000213 bp) in 2.150 CPU sec, 2.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285565, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.97, 130.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675066 reads in 275.454 CPU sec, 8.963 real sec
[M::mem_process_seqs] Processed 675100 reads in 151.131 CPU sec, 4.821 real sec
sorting block 198, #reads = 1080152 mem = 536880615 peak = 1314074624
[M::process] read 674930 sequences (100000177 bp) in 2.849 CPU sec, 3.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284916, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.51, 130.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 73, 788)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2312)
[M::mem_pestat] mean and std.dev: (387.69, 475.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3074)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 199, #reads = 1080154 mem = 536878721 peak = 1314074624
[M::process] read 674862 sequences (100000239 bp) in 1.907 CPU sec, 2.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284920, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.18, 131.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 146, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103)
[M::mem_pestat] mean and std.dev: (120.50, 125.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1462)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675104 reads in 279.965 CPU sec, 9.136 real sec
[M::mem_process_seqs] Processed 674944 reads in 156.574 CPU sec, 5.071 real sec
sorting block 200, #reads = 1079752 mem = 536880096 peak = 1314074624
[M::process] read 674978 sequences (100000191 bp) in 2.650 CPU sec, 3.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284618, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.08, 132.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674994 sequences (100000069 bp) in 2.141 CPU sec, 2.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285123, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.32, 134.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674930 reads in 273.333 CPU sec, 8.886 real sec
[M::mem_process_seqs] Processed 674862 reads in 153.842 CPU sec, 4.973 real sec
sorting block 201, #reads = 1079900 mem = 536881861 peak = 1314074624
[M::process] read 675132 sequences (100000247 bp) in 2.868 CPU sec, 3.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284681, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.55, 134.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675034 sequences (100000265 bp) in 2.093 CPU sec, 2.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285419, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (256.36, 134.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674978 reads in 281.809 CPU sec, 9.145 real sec
[M::mem_process_seqs] Processed 674994 reads in 162.318 CPU sec, 5.199 real sec
sorting block 202, #reads = 1080247 mem = 536878576 peak = 1314074624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285420, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (254.15, 131.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675092 sequences (100000178 bp) in 2.634 CPU sec, 3.391 real sec...
[M::process] read 674958 sequences (100000276 bp) in 2.187 CPU sec, 2.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285154, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (255.66, 133.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675132 reads in 257.201 CPU sec, 8.347 real sec
[M::mem_process_seqs] Processed 675034 reads in 146.952 CPU sec, 4.685 real sec
sorting block 203, #reads = 1076861 mem = 536879326 peak = 1314074624
sorting block 204, #reads = 1076667 mem = 536878787 peak = 1314549760
[M::process] read 675162 sequences (100000186 bp) in 2.886 CPU sec, 3.581 real sec...
[M::process] read 675486 sequences (100000135 bp) in 2.028 CPU sec, 2.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285413, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (259.89, 136.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674958 reads in 130.972 CPU sec, 4.265 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285491, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (258.44, 135.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 50, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1664)
[M::mem_pestat] mean and std.dev: (141.17, 198.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2214)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285438, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (255.58, 132.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675200 sequences (100000126 bp) in 2.097 CPU sec, 2.241 real sec...
[M::mem_process_seqs] Processed 675092 reads in 375.655 CPU sec, 12.241 real sec
[M::mem_process_seqs] Processed 675162 reads in 154.006 CPU sec, 4.968 real sec
sorting block 205, #reads = 1077349 mem = 536881687 peak = 1314549760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285283, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.27, 129.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675180 sequences (100000118 bp) in 2.693 CPU sec, 3.456 real sec...
[M::process] read 675338 sequences (100000029 bp) in 2.149 CPU sec, 2.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285551, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.35, 132.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 5, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 173)
[M::mem_pestat] mean and std.dev: (17.38, 23.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 229)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675486 reads in 262.724 CPU sec, 8.528 real sec
sorting block 206, #reads = 1078902 mem = 536879122 peak = 1314549760
[M::mem_process_seqs] Processed 675200 reads in 145.456 CPU sec, 4.650 real sec
sorting block 207, #reads = 1080049 mem = 536877907 peak = 1314549760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285801, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.32, 130.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675046 sequences (100000170 bp) in 2.559 CPU sec, 3.035 real sec...
[M::process] read 675098 sequences (100000228 bp) in 1.942 CPU sec, 2.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285451, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (254.46, 130.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675180 reads in 262.641 CPU sec, 8.600 real sec
[M::mem_process_seqs] Processed 675338 reads in 148.590 CPU sec, 4.820 real sec
sorting block 208, #reads = 1080156 mem = 536878479 peak = 1314549760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285246, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.55, 131.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675304 sequences (100000039 bp) in 2.629 CPU sec, 2.997 real sec...
[M::process] read 675482 sequences (100000161 bp) in 2.157 CPU sec, 2.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285762, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (259.15, 133.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 73, 9615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28729)
[M::mem_pestat] mean and std.dev: (3573.79, 4614.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38286)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675046 reads in 263.518 CPU sec, 8.569 real sec
[M::mem_process_seqs] Processed 675098 reads in 149.514 CPU sec, 4.805 real sec
sorting block 209, #reads = 1079649 mem = 536878782 peak = 1314549760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285699, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.01, 128.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 217, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165)
[M::mem_pestat] mean and std.dev: (298.18, 199.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1481)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675494 sequences (100000066 bp) in 2.434 CPU sec, 3.320 real sec...
[M::process] read 675442 sequences (100000228 bp) in 2.133 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285495, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.21, 124.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675304 reads in 255.144 CPU sec, 8.282 real sec
[M::mem_process_seqs] Processed 675482 reads in 142.421 CPU sec, 4.539 real sec
sorting block 210, #reads = 1079926 mem = 536879467 peak = 1314549760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285987, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.46, 124.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 211, #reads = 1079940 mem = 536877205 peak = 1325006848
[M::process] read 675480 sequences (100000121 bp) in 2.630 CPU sec, 3.395 real sec...
[M::process] read 675334 sequences (100000260 bp) in 2.212 CPU sec, 2.479 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286100, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.35, 126.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675494 reads in 255.872 CPU sec, 8.391 real sec
[M::mem_process_seqs] Processed 675442 reads in 147.323 CPU sec, 4.781 real sec
sorting block 212, #reads = 1079936 mem = 536878826 peak = 1325006848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285766, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.03, 123.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675168 sequences (100000096 bp) in 2.610 CPU sec, 3.306 real sec...
[M::process] read 675278 sequences (100000182 bp) in 2.065 CPU sec, 2.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286051, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.75, 122.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177)
[M::mem_pestat] mean and std.dev: (33.52, 40.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 235)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675480 reads in 249.319 CPU sec, 8.106 real sec
[M::mem_process_seqs] Processed 675334 reads in 140.426 CPU sec, 4.533 real sec
sorting block 213, #reads = 1080323 mem = 536878004 peak = 1325006848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285633, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.21, 125.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 39, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191)
[M::mem_pestat] mean and std.dev: (57.30, 65.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 320)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675214 sequences (100000148 bp) in 2.645 CPU sec, 2.864 real sec...
[M::process] read 675398 sequences (100000272 bp) in 2.133 CPU sec, 2.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285295, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.56, 128.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675168 reads in 256.376 CPU sec, 8.350 real sec
[M::mem_process_seqs] Processed 675278 reads in 146.462 CPU sec, 4.703 real sec
sorting block 214, #reads = 1079735 mem = 536883095 peak = 1325006848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285174, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.08, 127.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675188 sequences (100000095 bp) in 2.536 CPU sec, 3.298 real sec...
[M::process] read 675296 sequences (100000096 bp) in 2.025 CPU sec, 2.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285944, 13, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.07, 127.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (528, 528, 1362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3030)
[M::mem_pestat] mean and std.dev: (668.85, 502.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3864)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675214 reads in 248.206 CPU sec, 8.055 real sec
[M::mem_process_seqs] Processed 675398 reads in 135.051 CPU sec, 4.314 real sec
sorting block 215, #reads = 1079658 mem = 536882023 peak = 1325006848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285598, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.35, 129.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 39, 635)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1889)
[M::mem_pestat] mean and std.dev: (206.50, 289.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2516)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 216, #reads = 1078967 mem = 536878152 peak = 1325006848
[M::process] read 675038 sequences (100000291 bp) in 2.526 CPU sec, 3.035 real sec...
[M::process] read 675164 sequences (100000005 bp) in 2.192 CPU sec, 2.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284909, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.73, 127.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 1142, 2187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6439)
[M::mem_pestat] mean and std.dev: (1022.22, 922.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8565)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675188 reads in 260.263 CPU sec, 8.522 real sec
[M::mem_process_seqs] Processed 675296 reads in 152.395 CPU sec, 4.938 real sec
sorting block 217, #reads = 1075982 mem = 536879931 peak = 1325006848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284938, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.43, 127.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675288 sequences (100000136 bp) in 2.504 CPU sec, 3.191 real sec...
[M::process] read 675148 sequences (100000152 bp) in 2.111 CPU sec, 2.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285237, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.78, 124.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 36, 4614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13800)
[M::mem_pestat] mean and std.dev: (1167.29, 1916.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18393)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675038 reads in 261.537 CPU sec, 8.512 real sec
[M::mem_process_seqs] Processed 675164 reads in 145.647 CPU sec, 4.701 real sec
sorting block 218, #reads = 1079569 mem = 536881523 peak = 1325006848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285303, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.60, 125.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675074 sequences (100000013 bp) in 2.454 CPU sec, 3.552 real sec...
[M::process] read 675224 sequences (100000059 bp) in 2.137 CPU sec, 2.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285338, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.79, 124.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675288 reads in 263.880 CPU sec, 8.566 real sec
[M::mem_process_seqs] Processed 675148 reads in 147.023 CPU sec, 4.697 real sec
sorting block 219, #reads = 1080135 mem = 536878281 peak = 1325006848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285576, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.41, 124.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 281, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185)
[M::mem_pestat] mean and std.dev: (276.58, 199.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1551)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675200 sequences (100000027 bp) in 2.616 CPU sec, 3.371 real sec...
[M::process] read 675170 sequences (100000002 bp) in 2.006 CPU sec, 2.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285082, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.67, 126.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675074 reads in 269.382 CPU sec, 8.754 real sec
[M::mem_process_seqs] Processed 675224 reads in 152.350 CPU sec, 4.867 real sec
sorting block 220, #reads = 1080934 mem = 536880728 peak = 1325006848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285256, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 221, #reads = 1080522 mem = 536878837 peak = 1325006848
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.09, 127.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675244 sequences (100000034 bp) in 2.655 CPU sec, 3.197 real sec...
[M::process] read 675128 sequences (100000026 bp) in 2.068 CPU sec, 2.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285604, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.95, 128.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675200 reads in 273.632 CPU sec, 8.953 real sec
[M::mem_process_seqs] Processed 675170 reads in 153.220 CPU sec, 4.972 real sec
sorting block 222, #reads = 1080810 mem = 536878934 peak = 1325006848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285562, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.76, 130.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 88, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092)
[M::mem_pestat] mean and std.dev: (226.11, 329.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1545)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675172 sequences (100000290 bp) in 2.683 CPU sec, 3.171 real sec...
[M::process] read 675190 sequences (100000077 bp) in 2.020 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285353, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.73, 129.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675244 reads in 268.324 CPU sec, 8.734 real sec
[M::mem_process_seqs] Processed 675128 reads in 150.058 CPU sec, 4.850 real sec
sorting block 223, #reads = 1080552 mem = 536879705 peak = 1325006848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285271, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.00, 129.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675130 sequences (100000037 bp) in 2.394 CPU sec, 3.412 real sec...
[M::process] read 675070 sequences (100000283 bp) in 2.145 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284909, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.46, 130.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675172 reads in 271.183 CPU sec, 8.801 real sec
sorting block 224, #reads = 1080701 mem = 536878942 peak = 1325006848
[M::process] read 675204 sequences (100000024 bp) in 2.203 CPU sec, 2.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284916, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.15, 130.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675130 reads in 149.311 CPU sec, 4.882 real sec
[M::mem_process_seqs] Processed 675190 reads in 353.146 CPU sec, 11.424 real sec
sorting block 225, #reads = 1080975 mem = 536877804 peak = 1325006848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284743, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.02, 128.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675074 sequences (100000162 bp) in 2.411 CPU sec, 3.481 real sec...
[M::process] read 675038 sequences (100000180 bp) in 2.110 CPU sec, 2.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285057, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.38, 130.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675070 reads in 281.726 CPU sec, 9.125 real sec
[M::mem_process_seqs] Processed 675204 reads in 152.241 CPU sec, 4.877 real sec
sorting block 226, #reads = 1079949 mem = 536879511 peak = 1325006848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285296, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.28, 131.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 221, 7494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22098)
[M::mem_pestat] mean and std.dev: (2602.17, 3459.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 29400)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674986 sequences (100000165 bp) in 2.731 CPU sec, 3.466 real sec...
[M::process] read 675066 sequences (100000241 bp) in 2.079 CPU sec, 2.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285257, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.36, 133.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675074 reads in 271.669 CPU sec, 8.810 real sec
[M::mem_process_seqs] Processed 675038 reads in 151.896 CPU sec, 4.851 real sec
sorting block 227, #reads = 1080483 mem = 536877794 peak = 1325006848
[M::process] read 675030 sequences (100000126 bp) in 2.597 CPU sec, 2.930 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284836, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.40, 133.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 228, #reads = 1080950 mem = 536878471 peak = 1325019136
[M::process] read 675108 sequences (100000027 bp) in 1.970 CPU sec, 2.234 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285137, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.79, 134.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674986 reads in 273.534 CPU sec, 8.953 real sec
[M::mem_process_seqs] Processed 675066 reads in 152.458 CPU sec, 4.945 real sec
sorting block 229, #reads = 1080223 mem = 536876978 peak = 1325019136
[M::process] read 674958 sequences (100000183 bp) in 2.484 CPU sec, 3.007 real sec...
[M::process] read 674998 sequences (100000191 bp) in 2.191 CPU sec, 2.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285182, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.45, 135.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 339, 2074)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6008)
[M::mem_pestat] mean and std.dev: (723.12, 846.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7975)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675108 reads in 145.734 CPU sec, 4.700 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285466, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.49, 133.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674944 sequences (100000084 bp) in 2.023 CPU sec, 2.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285006, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.40, 136.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 9, 827)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2465)
[M::mem_pestat] mean and std.dev: (83.45, 235.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3284)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675030 reads in 409.482 CPU sec, 13.253 real sec
sorting block 230, #reads = 1080717 mem = 536880482 peak = 1325019136
[M::mem_process_seqs] Processed 674958 reads in 290.678 CPU sec, 9.352 real sec
sorting block 231, #reads = 1080207 mem = 536881026 peak = 1325195264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284669, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.47, 135.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 21, 37)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 77)
[M::mem_pestat] mean and std.dev: (24.00, 11.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 97)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674934 sequences (100000212 bp) in 2.656 CPU sec, 3.671 real sec...
[M::process] read 674796 sequences (100000141 bp) in 2.105 CPU sec, 2.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284967, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.48, 136.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674998 reads in 270.505 CPU sec, 8.854 real sec
[M::mem_process_seqs] Processed 674944 reads in 151.085 CPU sec, 4.902 real sec
sorting block 232, #reads = 1080051 mem = 536879669 peak = 1325195264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285007, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (260.55, 137.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674936 sequences (100000066 bp) in 2.571 CPU sec, 3.384 real sec...
[M::process] read 199558 sequences (29555541 bp) in 0.755 CPU sec, 0.757 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285125, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (261.15, 138.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674934 reads in 262.499 CPU sec, 8.568 real sec
[M::mem_process_seqs] Processed 674796 reads in 144.958 CPU sec, 4.696 real sec
sorting block 233, #reads = 1080192 mem = 536877618 peak = 1325195264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285108, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (259.88, 137.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 84363, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.14, 137.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674936 reads in 173.583 CPU sec, 5.738 real sec
[M::mem_process_seqs] Processed 199558 reads in 50.345 CPU sec, 1.627 real sec
sorting block 234, #reads = 1079772 mem = 536879243 peak = 1325195264
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050654
  features: 1f8bfbff fffa3203
  extended: d19f47ab 00000018
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8175M CPU @ 2.50GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 252010872
overall: 70036144128 mem 51763.193 user 318.045 sys 1718.226 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003322.L003\tPU:L003\tSM:T21-D0003322\tLB:T21-D0003322\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 T21-D0003322_R1.fastq.gz T21-D0003322_R2.fastq.gz
[main] Real time: 1718.255 sec; CPU: 52081.241 sec
sorting block 235, #reads = 620598 mem = 308595448 peak = 1325195264
uniq header count 1
merging 236 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050654
  features: 1f8bfbff fffa3203
  extended: d19f47ab 00000018
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8175M CPU @ 2.50GHz
threads: 31 max 32
algo: util-sort
license: sentieon:util=1
output file size: 8782949989
output reads: 254397868
bam_mem_sort: 236 calls 252.655 user 0.287 sys 268.435 real
bam_write: 236 calls 172.363 user 11.880 sys 469.654 real
execute: 1 calls 87.657 user 2.233 sys 1815.386 real
merge_files: 1 calls 87.615 user 2.201 sys 96.671 real
parse_chunk: 3770 calls 337.110 user 65.715 sys 528.418 real
read_chunk: 10145 calls 171.152 user 78.368 sys 1713.762 real
sort_block: 1 calls 113.172 user 76.952 sys 1645.826 real
write_chunk: 6373 calls 86.719 user 1.882 sys 93.208 real
overall: 3045609472 mem 4076.571 user 167.481 sys 1815.411 real
ls: cannot access '*.cram': No such file or directory