.command.log - Workdir - DAQ:SENTIEON_BWAMEM (T18-D0003255;L006;0004)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/58/609b44fedbfc9b0661d3a38aa7ae9b/.command.log
Size: 120.1 KB
Task: DAQ:SENTIEON_BWAMEM (T18-D0003255;L006;0004)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/58/609b44fedbfc9b0661d3a38aa7ae9b/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/43/bee6fe602e542585ff2c635192033d/T18-D0003255/0004.T18-D0003255_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/43/bee6fe602e542585ff2c635192033d/T18-D0003255/0004.T18-D0003255_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/58/609b44fedbfc9b0661d3a38aa7ae9b/.command.run
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1947.729 CPU sec, 371.748 real sec
[M::process] read 674090 sequences (100000199 bp) in 0.953 CPU sec, 0.959 real sec...
[M::process] read 674042 sequences (100000148 bp) in 1.519 CPU sec, 1.518 real sec...
[M::process] read 673862 sequences (100000175 bp) in 1.440 CPU sec, 1.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286858, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.72, 122.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673746 sequences (100000258 bp) in 1.399 CPU sec, 1.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286443, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (251.48, 123.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286268, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (254.22, 126.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674090 reads in 302.049 CPU sec, 9.754 real sec
[M::mem_process_seqs] Processed 674042 reads in 194.880 CPU sec, 6.411 real sec
[M::mem_process_seqs] Processed 673862 reads in 203.007 CPU sec, 6.700 real sec
sorting block 0, #reads = 1080525 mem = 536878536 peak = 597786624
[M::process] read 673708 sequences (100000280 bp) in 1.343 CPU sec, 1.446 real sec...
[M::process] read 673602 sequences (100000166 bp) in 1.234 CPU sec, 1.253 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286741, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (256.11, 127.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 16, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186)
[M::mem_pestat] mean and std.dev: (132.07, 266.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1579)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673418 sequences (100000056 bp) in 1.010 CPU sec, 1.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286315, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.09, 128.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (62, 384, 730)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2066)
[M::mem_pestat] mean and std.dev: (436.44, 442.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2734)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285849, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.56, 131.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673746 reads in 279.044 CPU sec, 8.965 real sec
sorting block 1, #reads = 1080609 mem = 536881161 peak = 863924224
[M::mem_process_seqs] Processed 673708 reads in 193.654 CPU sec, 6.270 real sec
[M::mem_process_seqs] Processed 673602 reads in 202.040 CPU sec, 6.568 real sec
sorting block 2, #reads = 1080701 mem = 536877137 peak = 887848960
[M::process] read 673350 sequences (100000011 bp) in 2.303 CPU sec, 2.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285355, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.83, 133.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673346 sequences (100000237 bp) in 1.710 CPU sec, 1.760 real sec...
[M::process] read 673206 sequences (100000258 bp) in 1.320 CPU sec, 1.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285472, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.06, 135.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673418 reads in 199.688 CPU sec, 6.378 real sec
[M::mem_process_seqs] Processed 673350 reads in 109.059 CPU sec, 3.465 real sec
sorting block 3, #reads = 1079872 mem = 536882301 peak = 887848960
sorting block 4, #reads = 1079553 mem = 536881189 peak = 887848960
[M::process] read 673246 sequences (100000187 bp) in 1.827 CPU sec, 1.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285551, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.49, 136.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (424, 649, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (512.67, 191.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1324)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673184 sequences (100000194 bp) in 1.340 CPU sec, 1.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285429, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.16, 139.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (70, 649, 1509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4387)
[M::mem_pestat] mean and std.dev: (669.23, 608.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5826)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285770, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.29, 140.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 283, 844)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2272)
[M::mem_pestat] mean and std.dev: (390.79, 341.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2986)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673346 reads in 283.349 CPU sec, 9.101 real sec
[M::mem_process_seqs] Processed 673206 reads in 195.356 CPU sec, 6.319 real sec
[M::mem_process_seqs] Processed 673246 reads in 203.538 CPU sec, 6.616 real sec
sorting block 5, #reads = 1079588 mem = 536880739 peak = 887848960
[M::process] read 672966 sequences (100000240 bp) in 1.668 CPU sec, 1.795 real sec...
[M::process] read 672876 sequences (100000069 bp) in 1.308 CPU sec, 1.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285119, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.41, 140.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 73, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191)
[M::mem_pestat] mean and std.dev: (58.25, 32.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 243)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672908 sequences (100000152 bp) in 1.030 CPU sec, 1.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284700, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.02, 142.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285478, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.01, 145.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 93, 114)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284)
[M::mem_pestat] mean and std.dev: (76.79, 55.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 369)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673184 reads in 293.681 CPU sec, 9.431 real sec
sorting block 6, #reads = 1080041 mem = 536878968 peak = 887848960
[M::mem_process_seqs] Processed 672966 reads in 200.778 CPU sec, 6.496 real sec
[M::mem_process_seqs] Processed 672876 reads in 209.265 CPU sec, 6.798 real sec
sorting block 7, #reads = 1079703 mem = 536880081 peak = 895340544
[M::process] read 672732 sequences (100000289 bp) in 2.069 CPU sec, 2.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285438, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.14, 146.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673718 sequences (100000259 bp) in 1.639 CPU sec, 1.680 real sec...
[M::process] read 673892 sequences (100000131 bp) in 1.380 CPU sec, 1.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285904, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.68, 147.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672908 reads in 194.754 CPU sec, 6.217 real sec
[M::mem_process_seqs] Processed 672732 reads in 106.160 CPU sec, 3.389 real sec
sorting block 8, #reads = 1079458 mem = 536884776 peak = 895340544
sorting block 9, #reads = 1079625 mem = 536879986 peak = 928227328
[M::process] read 673804 sequences (100000273 bp) in 1.873 CPU sec, 1.964 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286597, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.09, 128.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 162, 893)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2647)
[M::mem_pestat] mean and std.dev: (348.00, 460.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3524)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673706 sequences (100000228 bp) in 1.409 CPU sec, 1.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286450, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (253.64, 125.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286090, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.62, 127.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673718 reads in 279.394 CPU sec, 8.982 real sec
[M::mem_process_seqs] Processed 673892 reads in 197.388 CPU sec, 6.383 real sec
[M::mem_process_seqs] Processed 673804 reads in 205.873 CPU sec, 6.679 real sec
sorting block 10, #reads = 1080714 mem = 536878725 peak = 928227328
[M::process] read 673700 sequences (100000063 bp) in 1.548 CPU sec, 1.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285418, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (256.45, 127.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673534 sequences (100000111 bp) in 1.856 CPU sec, 1.942 real sec...
[M::process] read 673388 sequences (100000211 bp) in 1.419 CPU sec, 1.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285897, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.99, 129.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 1918, 1918)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5282)
[M::mem_pestat] mean and std.dev: (1590.57, 1360.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7033)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673706 reads in 188.294 CPU sec, 6.017 real sec
sorting block 11, #reads = 1080208 mem = 536877918 peak = 928227328
[M::mem_process_seqs] Processed 673700 reads in 104.146 CPU sec, 3.356 real sec
sorting block 12, #reads = 1079649 mem = 536879377 peak = 928227328
[M::process] read 673364 sequences (100000096 bp) in 1.874 CPU sec, 1.975 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285793, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.37, 131.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673382 sequences (100000229 bp) in 1.430 CPU sec, 1.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285159, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.65, 134.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285664, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.34, 135.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673534 reads in 275.444 CPU sec, 8.840 real sec
[M::mem_process_seqs] Processed 673388 reads in 195.227 CPU sec, 6.299 real sec
[M::mem_process_seqs] Processed 673364 reads in 204.383 CPU sec, 6.621 real sec
sorting block 13, #reads = 1080044 mem = 536877813 peak = 928227328
[M::process] read 673148 sequences (100000000 bp) in 1.964 CPU sec, 2.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285412, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.55, 135.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 86, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216)
[M::mem_pestat] mean and std.dev: (190.07, 206.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1617)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 14, #reads = 1080110 mem = 536879367 peak = 928227328
[M::process] read 673178 sequences (100000071 bp) in 1.656 CPU sec, 1.772 real sec...
[M::process] read 673162 sequences (100000080 bp) in 1.433 CPU sec, 1.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285461, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.00, 138.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673382 reads in 203.272 CPU sec, 6.505 real sec
[M::mem_process_seqs] Processed 673148 reads in 115.410 CPU sec, 3.691 real sec
sorting block 15, #reads = 1080165 mem = 536877587 peak = 928227328
[M::process] read 673004 sequences (100000141 bp) in 1.890 CPU sec, 2.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285220, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.72, 140.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672880 sequences (100000292 bp) in 1.430 CPU sec, 1.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285291, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.07, 142.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285409, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.94, 142.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (55, 198, 691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1963)
[M::mem_pestat] mean and std.dev: (211.55, 238.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2599)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673178 reads in 277.112 CPU sec, 8.889 real sec
[M::mem_process_seqs] Processed 673004 reads in 191.762 CPU sec, 6.194 real sec
[M::mem_process_seqs] Processed 673162 reads in 200.578 CPU sec, 6.508 real sec
sorting block 16, #reads = 1079862 mem = 536878855 peak = 928227328
sorting block 17, #reads = 1079896 mem = 536878088 peak = 928227328
[M::process] read 672690 sequences (100000023 bp) in 2.544 CPU sec, 2.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285596, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.62, 143.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 73, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1797)
[M::mem_pestat] mean and std.dev: (251.00, 299.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2391)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672820 sequences (100000271 bp) in 1.528 CPU sec, 1.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285363, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.02, 147.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 158, 191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (87.78, 85.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673444 sequences (100000069 bp) in 1.470 CPU sec, 1.467 real sec...
[M::mem_process_seqs] Processed 672880 reads in 184.707 CPU sec, 5.905 real sec
[M::mem_process_seqs] Processed 672690 reads in 105.376 CPU sec, 3.352 real sec
sorting block 18, #reads = 1079883 mem = 536877911 peak = 928227328
[M::process] read 673944 sequences (100000082 bp) in 2.264 CPU sec, 2.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285367, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.14, 148.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673966 sequences (100000169 bp) in 1.120 CPU sec, 1.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286277, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.22, 134.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286363, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (252.13, 124.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 22, 24)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 66)
[M::mem_pestat] mean and std.dev: (14.83, 10.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 87)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672820 reads in 256.743 CPU sec, 8.231 real sec
[M::mem_process_seqs] Processed 673444 reads in 179.901 CPU sec, 5.802 real sec
[M::mem_process_seqs] Processed 673944 reads in 189.186 CPU sec, 6.131 real sec
sorting block 19, #reads = 1079548 mem = 536877529 peak = 928227328
sorting block 20, #reads = 1079857 mem = 536880789 peak = 928227328
[M::process] read 673666 sequences (100000008 bp) in 1.978 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286343, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (255.17, 126.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673668 sequences (100000030 bp) in 1.393 CPU sec, 1.458 real sec...
[M::process] read 673580 sequences (100000083 bp) in 1.120 CPU sec, 1.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286150, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.43, 128.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (48, 97, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513)
[M::mem_pestat] mean and std.dev: (82.78, 65.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673966 reads in 180.841 CPU sec, 5.781 real sec
[M::mem_process_seqs] Processed 673666 reads in 101.684 CPU sec, 3.258 real sec
sorting block 21, #reads = 1080319 mem = 536877788 peak = 928227328
[M::process] read 673496 sequences (100000287 bp) in 2.062 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285556, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.55, 129.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 53, 792)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2312)
[M::mem_pestat] mean and std.dev: (349.73, 611.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3072)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673350 sequences (100000237 bp) in 1.100 CPU sec, 1.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286175, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (261.09, 131.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (92, 463, 1918)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5570)
[M::mem_pestat] mean and std.dev: (984.14, 1422.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7396)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285274, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.21, 133.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673668 reads in 265.080 CPU sec, 8.504 real sec
[M::mem_process_seqs] Processed 673580 reads in 186.828 CPU sec, 6.034 real sec
sorting block 22, #reads = 1079721 mem = 536879926 peak = 928227328
[M::mem_process_seqs] Processed 673496 reads in 196.572 CPU sec, 6.378 real sec
sorting block 23, #reads = 1080453 mem = 536877670 peak = 928227328
[M::process] read 673348 sequences (100000183 bp) in 1.824 CPU sec, 1.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285065, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.76, 135.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 185, 1049)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3079)
[M::mem_pestat] mean and std.dev: (444.92, 460.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4094)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673086 sequences (100000131 bp) in 1.544 CPU sec, 1.659 real sec...
[M::process] read 673188 sequences (100000006 bp) in 1.100 CPU sec, 1.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285449, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.31, 134.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673350 reads in 186.857 CPU sec, 5.980 real sec
[M::mem_process_seqs] Processed 673348 reads in 105.192 CPU sec, 3.377 real sec
sorting block 24, #reads = 1079713 mem = 536878499 peak = 928227328
[M::process] read 672956 sequences (100000144 bp) in 2.208 CPU sec, 2.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285437, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.41, 141.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (67, 392, 911)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2599)
[M::mem_pestat] mean and std.dev: (419.95, 372.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3443)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673062 sequences (100000165 bp) in 1.267 CPU sec, 1.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285290, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.11, 140.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284989, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.98, 142.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673086 reads in 270.427 CPU sec, 8.681 real sec
[M::mem_process_seqs] Processed 673188 reads in 191.822 CPU sec, 6.192 real sec
sorting block 25, #reads = 1079712 mem = 536879447 peak = 928227328
[M::mem_process_seqs] Processed 672956 reads in 202.313 CPU sec, 6.563 real sec
sorting block 26, #reads = 1079711 mem = 536877799 peak = 928227328
[M::process] read 672904 sequences (100000176 bp) in 1.876 CPU sec, 2.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285198, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (275.60, 144.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672730 sequences (100000116 bp) in 1.508 CPU sec, 1.615 real sec...
[M::process] read 672668 sequences (100000091 bp) in 1.090 CPU sec, 1.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284936, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.50, 145.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673062 reads in 187.768 CPU sec, 6.018 real sec
[M::mem_process_seqs] Processed 672904 reads in 106.414 CPU sec, 3.410 real sec
sorting block 27, #reads = 1079746 mem = 536881895 peak = 928227328
[M::process] read 673208 sequences (100000215 bp) in 1.791 CPU sec, 1.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284752, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.23, 146.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 180, 1017)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2985)
[M::mem_pestat] mean and std.dev: (367.36, 477.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3969)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673954 sequences (100000153 bp) in 1.090 CPU sec, 1.094 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284874, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (283.07, 149.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285597, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.50, 140.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 698, 1366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4068)
[M::mem_pestat] mean and std.dev: (618.46, 602.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5419)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672730 reads in 263.214 CPU sec, 8.457 real sec
sorting block 28, #reads = 1079708 mem = 536879452 peak = 928227328
[M::mem_process_seqs] Processed 672668 reads in 184.543 CPU sec, 5.978 real sec
[M::mem_process_seqs] Processed 673208 reads in 194.402 CPU sec, 6.327 real sec
sorting block 29, #reads = 1079377 mem = 536879256 peak = 964927488
[M::process] read 673878 sequences (100000227 bp) in 2.046 CPU sec, 2.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286157, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (250.65, 122.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673766 sequences (100000158 bp) in 1.724 CPU sec, 1.850 real sec...
[M::process] read 673704 sequences (100000292 bp) in 1.130 CPU sec, 1.132 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285825, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (253.01, 124.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 844, 844)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2360)
[M::mem_pestat] mean and std.dev: (772.10, 841.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4137)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673954 reads in 180.621 CPU sec, 5.803 real sec
sorting block 30, #reads = 1079877 mem = 536878496 peak = 964927488
[M::mem_process_seqs] Processed 673878 reads in 103.530 CPU sec, 3.308 real sec
sorting block 31, #reads = 1080274 mem = 536879101 peak = 964927488
[M::process] read 673166 sequences (100000032 bp) in 2.133 CPU sec, 2.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286017, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (255.55, 126.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 25, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236)
[M::mem_pestat] mean and std.dev: (35.77, 35.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 311)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673422 sequences (100000057 bp) in 1.150 CPU sec, 1.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285145, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.61, 137.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 189, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (87.30, 84.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 713)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286066, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.76, 128.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 21, 98)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288)
[M::mem_pestat] mean and std.dev: (61.73, 88.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 416)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673766 reads in 264.277 CPU sec, 8.486 real sec
[M::mem_process_seqs] Processed 673166 reads in 186.638 CPU sec, 6.028 real sec
[M::mem_process_seqs] Processed 673704 reads in 196.115 CPU sec, 6.362 real sec
sorting block 32, #reads = 1079775 mem = 536880899 peak = 964927488
[M::process] read 673290 sequences (100000198 bp) in 2.351 CPU sec, 2.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285162, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (261.11, 130.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 39, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096)
[M::mem_pestat] mean and std.dev: (181.27, 215.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1456)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673390 sequences (100000114 bp) in 1.371 CPU sec, 1.423 real sec...
[M::process] read 673100 sequences (100000020 bp) in 1.150 CPU sec, 1.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285015, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.01, 133.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673422 reads in 187.412 CPU sec, 6.011 real sec
sorting block 33, #reads = 1079990 mem = 536876723 peak = 964927488
[M::mem_process_seqs] Processed 673290 reads in 107.592 CPU sec, 3.442 real sec
sorting block 34, #reads = 1079574 mem = 536879096 peak = 975028224
[M::process] read 673108 sequences (100000165 bp) in 2.273 CPU sec, 2.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285254, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.33, 132.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673044 sequences (100000164 bp) in 1.120 CPU sec, 1.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284987, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.11, 137.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 85, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (101.46, 86.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285194, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.35, 137.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673390 reads in 271.806 CPU sec, 8.728 real sec
[M::mem_process_seqs] Processed 673100 reads in 191.248 CPU sec, 6.173 real sec
[M::mem_process_seqs] Processed 673108 reads in 200.841 CPU sec, 6.513 real sec
sorting block 35, #reads = 1080102 mem = 536880472 peak = 975028224
[M::process] read 672780 sequences (100000100 bp) in 1.949 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285308, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.19, 140.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 75, 651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1895)
[M::mem_pestat] mean and std.dev: (192.69, 240.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2517)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 36, #reads = 1079676 mem = 536879996 peak = 975028224
[M::process] read 672926 sequences (100000257 bp) in 1.774 CPU sec, 1.897 real sec...
[M::process] read 672716 sequences (100000225 bp) in 1.170 CPU sec, 1.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284636, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.55, 144.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 463, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217)
[M::mem_pestat] mean and std.dev: (296.64, 223.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1594)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673044 reads in 193.786 CPU sec, 6.203 real sec
[M::mem_process_seqs] Processed 672780 reads in 110.635 CPU sec, 3.547 real sec
sorting block 37, #reads = 1080216 mem = 536877715 peak = 975028224
[M::process] read 672770 sequences (100000181 bp) in 2.143 CPU sec, 2.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284869, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.44, 143.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 148, 1087)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3213)
[M::mem_pestat] mean and std.dev: (470.20, 505.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4276)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672676 sequences (100000071 bp) in 1.120 CPU sec, 1.129 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284365, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.39, 147.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284864, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.73, 148.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672926 reads in 272.467 CPU sec, 8.751 real sec
[M::mem_process_seqs] Processed 672716 reads in 192.454 CPU sec, 6.220 real sec
[M::mem_process_seqs] Processed 672770 reads in 202.265 CPU sec, 6.566 real sec
sorting block 38, #reads = 1079701 mem = 536879567 peak = 975028224
[M::process] read 674004 sequences (100000061 bp) in 2.418 CPU sec, 2.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285282, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (282.23, 147.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 122, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (89.38, 78.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 39, #reads = 1079044 mem = 536877705 peak = 975028224
[M::process] read 674152 sequences (100000244 bp) in 1.630 CPU sec, 1.716 real sec...
[M::process] read 673992 sequences (100000141 bp) in 1.170 CPU sec, 1.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286469, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.38, 128.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672676 reads in 185.705 CPU sec, 5.951 real sec
[M::mem_process_seqs] Processed 674004 reads in 107.541 CPU sec, 3.435 real sec
sorting block 40, #reads = 1079519 mem = 536879653 peak = 975028224
[M::process] read 673804 sequences (100000036 bp) in 2.095 CPU sec, 2.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285862, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.21, 126.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673958 sequences (100000221 bp) in 1.160 CPU sec, 1.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285656, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (253.76, 127.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285633, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (254.14, 128.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 41, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (127.44, 179.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674152 reads in 266.263 CPU sec, 8.548 real sec
[M::mem_process_seqs] Processed 673992 reads in 189.332 CPU sec, 6.112 real sec
[M::mem_process_seqs] Processed 673804 reads in 199.104 CPU sec, 6.460 real sec
sorting block 41, #reads = 1079945 mem = 536875830 peak = 975028224
[M::process] read 673666 sequences (100000071 bp) in 2.192 CPU sec, 2.383 real sec...
sorting block 42, #reads = 1080239 mem = 536878796 peak = 975028224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285584, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.34, 130.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673698 sequences (100000052 bp) in 1.622 CPU sec, 1.717 real sec...
[M::process] read 673500 sequences (100000222 bp) in 1.180 CPU sec, 1.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284915, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.57, 133.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673958 reads in 192.304 CPU sec, 6.165 real sec
[M::mem_process_seqs] Processed 673666 reads in 110.948 CPU sec, 3.549 real sec
sorting block 43, #reads = 1080447 mem = 536880219 peak = 975028224
[M::process] read 673324 sequences (100000020 bp) in 1.842 CPU sec, 1.953 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284943, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.08, 135.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673336 sequences (100000114 bp) in 1.160 CPU sec, 1.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284606, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.67, 136.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285027, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.50, 138.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673698 reads in 275.204 CPU sec, 8.838 real sec
[M::mem_process_seqs] Processed 673500 reads in 193.932 CPU sec, 6.276 real sec
[M::mem_process_seqs] Processed 673324 reads in 203.692 CPU sec, 6.622 real sec
sorting block 44, #reads = 1079819 mem = 536877390 peak = 975028224
sorting block 45, #reads = 1079906 mem = 536878756 peak = 975028224
[M::process] read 673284 sequences (100000019 bp) in 2.192 CPU sec, 2.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285170, 11, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.40, 138.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 621, 3133)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9011)
[M::mem_pestat] mean and std.dev: (1133.27, 1272.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11950)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673216 sequences (100000055 bp) in 1.626 CPU sec, 1.700 real sec...
[M::process] read 673140 sequences (100000083 bp) in 1.180 CPU sec, 1.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285045, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.96, 138.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673336 reads in 193.008 CPU sec, 6.178 real sec
[M::mem_process_seqs] Processed 673284 reads in 108.704 CPU sec, 3.477 real sec
sorting block 46, #reads = 1079345 mem = 536878322 peak = 975028224
[M::process] read 673168 sequences (100000087 bp) in 2.449 CPU sec, 2.635 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284837, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.70, 141.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673086 sequences (100000225 bp) in 1.550 CPU sec, 1.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284485, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.61, 142.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284635, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.19, 144.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673216 reads in 276.797 CPU sec, 8.870 real sec
[M::mem_process_seqs] Processed 673140 reads in 193.716 CPU sec, 6.234 real sec
sorting block 47, #reads = 1080107 mem = 536877703 peak = 975028224
[M::mem_process_seqs] Processed 673168 reads in 203.293 CPU sec, 6.572 real sec
sorting block 48, #reads = 1079564 mem = 536879756 peak = 975028224
[M::process] read 673384 sequences (100000188 bp) in 2.261 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284953, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (277.28, 147.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674234 sequences (100000011 bp) in 1.639 CPU sec, 1.727 real sec...
[M::process] read 674032 sequences (100000240 bp) in 1.180 CPU sec, 1.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285257, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (267.84, 140.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 353, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303)
[M::mem_pestat] mean and std.dev: (251.00, 224.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1729)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673086 reads in 192.936 CPU sec, 6.175 real sec
[M::mem_process_seqs] Processed 673384 reads in 107.185 CPU sec, 3.432 real sec
sorting block 49, #reads = 1079785 mem = 536879918 peak = 975028224
[M::process] read 673916 sequences (100000173 bp) in 2.080 CPU sec, 2.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285987, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.86, 125.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673798 sequences (100000099 bp) in 1.150 CPU sec, 1.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286144, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (253.27, 126.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285997, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.05, 128.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (68, 281, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (256.00, 187.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674234 reads in 262.023 CPU sec, 8.413 real sec
sorting block 50, #reads = 1079664 mem = 536878501 peak = 975028224
[M::mem_process_seqs] Processed 674032 reads in 185.137 CPU sec, 5.979 real sec
[M::mem_process_seqs] Processed 673916 reads in 194.926 CPU sec, 6.324 real sec
sorting block 51, #reads = 1080229 mem = 536879054 peak = 1032134656
[M::process] read 673644 sequences (100000222 bp) in 2.342 CPU sec, 2.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285840, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.11, 130.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673714 sequences (100000075 bp) in 1.605 CPU sec, 1.713 real sec...
[M::process] read 673602 sequences (100000222 bp) in 1.200 CPU sec, 1.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285219, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.56, 131.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 31, 106)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312)
[M::mem_pestat] mean and std.dev: (35.55, 37.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 415)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673798 reads in 192.023 CPU sec, 6.155 real sec
sorting block 52, #reads = 1080040 mem = 536879755 peak = 1032134656
[M::mem_process_seqs] Processed 673644 reads in 109.540 CPU sec, 3.498 real sec
sorting block 53, #reads = 1080102 mem = 536877538 peak = 1032134656
[M::process] read 673632 sequences (100000130 bp) in 2.137 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285254, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.59, 133.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673570 sequences (100000088 bp) in 1.160 CPU sec, 1.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285125, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.14, 136.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285433, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.77, 135.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673714 reads in 274.328 CPU sec, 8.807 real sec
[M::mem_process_seqs] Processed 673602 reads in 193.935 CPU sec, 6.259 real sec
[M::mem_process_seqs] Processed 673632 reads in 203.763 CPU sec, 6.606 real sec
sorting block 54, #reads = 1079856 mem = 536878448 peak = 1032134656
[M::process] read 673338 sequences (100000010 bp) in 2.150 CPU sec, 2.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284584, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.52, 139.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673208 sequences (100000118 bp) in 1.724 CPU sec, 1.839 real sec...
[M::process] read 673222 sequences (100000086 bp) in 1.160 CPU sec, 1.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284629, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.42, 140.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673570 reads in 196.058 CPU sec, 6.274 real sec
sorting block 55, #reads = 1079592 mem = 536877761 peak = 1032134656
[M::mem_process_seqs] Processed 673338 reads in 111.525 CPU sec, 3.571 real sec
sorting block 56, #reads = 1079603 mem = 536877383 peak = 1050001408
[M::process] read 673034 sequences (100000263 bp) in 2.232 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284671, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.79, 142.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 29, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 169)
[M::mem_pestat] mean and std.dev: (25.11, 19.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 219)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673112 sequences (100000186 bp) in 1.160 CPU sec, 1.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284262, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.51, 145.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 675, 1509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4101)
[M::mem_pestat] mean and std.dev: (801.45, 761.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5397)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283704, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.88, 143.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673208 reads in 285.067 CPU sec, 9.155 real sec
[M::mem_process_seqs] Processed 673034 reads in 200.249 CPU sec, 6.470 real sec
[M::mem_process_seqs] Processed 673222 reads in 209.851 CPU sec, 6.809 real sec
sorting block 57, #reads = 1079386 mem = 536879274 peak = 1050001408
[M::process] read 673208 sequences (100000140 bp) in 2.335 CPU sec, 2.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284690, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (276.40, 148.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1079857 mem = 536878807 peak = 1050001408
[M::process] read 674024 sequences (100000293 bp) in 1.531 CPU sec, 1.608 real sec...
[M::process] read 673992 sequences (100000281 bp) in 1.180 CPU sec, 1.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284671, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (277.66, 150.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673112 reads in 196.458 CPU sec, 6.317 real sec
[M::mem_process_seqs] Processed 673208 reads in 111.477 CPU sec, 3.569 real sec
sorting block 59, #reads = 1079669 mem = 536878758 peak = 1050001408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285775, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.74, 127.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673948 sequences (100000044 bp) in 2.319 CPU sec, 2.530 real sec...
[M::process] read 673960 sequences (100000030 bp) in 1.140 CPU sec, 1.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285956, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (252.95, 126.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674024 reads in 184.042 CPU sec, 5.891 real sec
[M::mem_process_seqs] Processed 673992 reads in 105.133 CPU sec, 3.390 real sec
sorting block 60, #reads = 1079896 mem = 536878315 peak = 1050001408
[M::process] read 673866 sequences (100000154 bp) in 1.889 CPU sec, 2.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285868, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (254.67, 128.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 13)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 33)
[M::mem_pestat] mean and std.dev: (8.11, 9.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 47)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673710 sequences (100000033 bp) in 1.160 CPU sec, 1.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285086, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.08, 132.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285296, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.36, 130.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673948 reads in 262.522 CPU sec, 8.428 real sec
[M::mem_process_seqs] Processed 673866 reads in 184.229 CPU sec, 5.954 real sec
sorting block 61, #reads = 1080554 mem = 536878392 peak = 1050001408
[M::mem_process_seqs] Processed 673960 reads in 192.995 CPU sec, 6.264 real sec
sorting block 62, #reads = 1080209 mem = 536876906 peak = 1050001408
[M::process] read 673470 sequences (100000257 bp) in 2.031 CPU sec, 2.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285353, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.62, 134.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 65, 1876)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5556)
[M::mem_pestat] mean and std.dev: (254.78, 573.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7396)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673548 sequences (100000014 bp) in 1.302 CPU sec, 1.320 real sec...
[M::process] read 673430 sequences (100000052 bp) in 1.150 CPU sec, 1.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284572, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.21, 137.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673710 reads in 182.225 CPU sec, 5.839 real sec
[M::mem_process_seqs] Processed 673470 reads in 104.338 CPU sec, 3.336 real sec
sorting block 63, #reads = 1080131 mem = 536880005 peak = 1050001408
[M::process] read 673372 sequences (100000273 bp) in 1.875 CPU sec, 2.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285095, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.05, 135.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673284 sequences (100000103 bp) in 1.130 CPU sec, 1.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284498, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.41, 136.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284528, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.55, 140.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 81, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290)
[M::mem_pestat] mean and std.dev: (225.20, 320.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1719)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673548 reads in 260.781 CPU sec, 8.370 real sec
[M::mem_process_seqs] Processed 673430 reads in 184.250 CPU sec, 5.946 real sec
sorting block 64, #reads = 1079712 mem = 536877274 peak = 1050001408
[M::mem_process_seqs] Processed 673372 reads in 192.892 CPU sec, 6.254 real sec
sorting block 65, #reads = 1079814 mem = 536878331 peak = 1050001408
[M::process] read 673172 sequences (100000018 bp) in 2.139 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284674, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.14, 143.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 122, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (228.50, 278.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1341)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672996 sequences (100000206 bp) in 1.369 CPU sec, 1.416 real sec...
[M::process] read 673052 sequences (100000267 bp) in 1.140 CPU sec, 1.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284013, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.49, 143.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673284 reads in 182.981 CPU sec, 5.860 real sec
[M::mem_process_seqs] Processed 673172 reads in 103.634 CPU sec, 3.312 real sec
sorting block 66, #reads = 1079743 mem = 536876758 peak = 1050001408
[M::process] read 673112 sequences (100000149 bp) in 1.696 CPU sec, 1.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283973, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.19, 145.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673424 sequences (100000068 bp) in 1.170 CPU sec, 1.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284275, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (276.09, 147.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284510, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (278.54, 149.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 41, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (156.90, 210.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672996 reads in 262.150 CPU sec, 8.416 real sec
sorting block 67, #reads = 1079710 mem = 536879018 peak = 1050001408
[M::mem_process_seqs] Processed 673052 reads in 182.963 CPU sec, 5.911 real sec
[M::mem_process_seqs] Processed 673112 reads in 191.427 CPU sec, 6.213 real sec
sorting block 68, #reads = 1079278 mem = 536878091 peak = 1050353664
[M::process] read 674144 sequences (100000040 bp) in 1.836 CPU sec, 2.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285460, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (266.62, 140.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 70, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569)
[M::mem_pestat] mean and std.dev: (293.69, 293.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2084)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674136 sequences (100000195 bp) in 1.420 CPU sec, 1.477 real sec...
[M::process] read 673896 sequences (100000163 bp) in 1.160 CPU sec, 1.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286227, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.15, 125.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 117, 969)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2749)
[M::mem_pestat] mean and std.dev: (476.86, 490.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3639)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673424 reads in 170.920 CPU sec, 5.459 real sec
[M::mem_process_seqs] Processed 674144 reads in 94.809 CPU sec, 3.030 real sec
sorting block 69, #reads = 1078906 mem = 536876866 peak = 1050353664
[M::process] read 673784 sequences (100000037 bp) in 1.682 CPU sec, 1.825 real sec...
[M::process] read 673476 sequences (100000271 bp) in 1.160 CPU sec, 1.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285294, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.64, 129.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 24, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1227)
[M::mem_pestat] mean and std.dev: (57.50, 120.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1632)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673896 reads in 92.096 CPU sec, 2.957 real sec
sorting block 70, #reads = 1080493 mem = 536879103 peak = 1082265600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285634, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (252.70, 126.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673478 sequences (100000234 bp) in 1.181 CPU sec, 1.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285226, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.65, 132.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (65, 586, 1172)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3386)
[M::mem_pestat] mean and std.dev: (694.45, 727.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4493)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674136 reads in 261.506 CPU sec, 8.373 real sec
[M::mem_process_seqs] Processed 673784 reads in 187.037 CPU sec, 6.014 real sec
sorting block 71, #reads = 1079939 mem = 536878658 peak = 1100775424
[M::process] read 673422 sequences (100000033 bp) in 1.698 CPU sec, 1.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284642, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (267.91, 140.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673270 sequences (100000091 bp) in 1.090 CPU sec, 1.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284425, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.43, 135.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673476 reads in 177.580 CPU sec, 5.689 real sec
sorting block 72, #reads = 1079685 mem = 536878641 peak = 1100775424
[M::mem_process_seqs] Processed 673478 reads in 99.039 CPU sec, 3.202 real sec
sorting block 73, #reads = 1079617 mem = 536878531 peak = 1100775424
[M::process] read 673348 sequences (100000207 bp) in 1.661 CPU sec, 1.781 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284863, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.49, 136.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673326 sequences (100000068 bp) in 1.100 CPU sec, 1.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284430, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.49, 138.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284622, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (267.99, 140.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 68, 1017)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2979)
[M::mem_pestat] mean and std.dev: (404.25, 529.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3960)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673422 reads in 257.989 CPU sec, 8.283 real sec
[M::mem_process_seqs] Processed 673270 reads in 180.061 CPU sec, 5.828 real sec
[M::mem_process_seqs] Processed 673348 reads in 188.491 CPU sec, 6.127 real sec
sorting block 74, #reads = 1079866 mem = 536879939 peak = 1100775424
[M::process] read 673040 sequences (100000266 bp) in 1.894 CPU sec, 2.137 real sec...
sorting block 75, #reads = 1079175 mem = 536878648 peak = 1100775424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284481, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.12, 142.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673222 sequences (100000057 bp) in 1.274 CPU sec, 1.321 real sec...
[M::process] read 672974 sequences (100000027 bp) in 1.150 CPU sec, 1.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283915, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.97, 146.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673326 reads in 183.500 CPU sec, 5.870 real sec
[M::mem_process_seqs] Processed 673040 reads in 103.507 CPU sec, 3.316 real sec
sorting block 76, #reads = 1079306 mem = 536878632 peak = 1100775424
[M::process] read 672810 sequences (100000246 bp) in 1.720 CPU sec, 1.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284092, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.77, 145.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672938 sequences (100000154 bp) in 1.130 CPU sec, 1.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283683, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (280.49, 150.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (619, 881, 881)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (95, 1405)
[M::mem_pestat] mean and std.dev: (666.82, 277.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1778)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283878, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (283.24, 152.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 24, 1106)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3270)
[M::mem_pestat] mean and std.dev: (354.45, 470.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4352)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673222 reads in 265.681 CPU sec, 8.530 real sec
[M::mem_process_seqs] Processed 672974 reads in 186.769 CPU sec, 6.038 real sec
[M::mem_process_seqs] Processed 672810 reads in 195.388 CPU sec, 6.345 real sec
sorting block 77, #reads = 1079913 mem = 536879387 peak = 1100775424
[M::process] read 155998 sequences (23186851 bp) in 0.672 CPU sec, 0.717 real sec...
sorting block 78, #reads = 1078626 mem = 536876753 peak = 1129656320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283983, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (280.30, 151.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 66051, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (283.25, 154.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672938 reads in 113.637 CPU sec, 3.710 real sec
[M::mem_process_seqs] Processed 155998 reads in 29.669 CPU sec, 0.973 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 85679204
overall: 69801127936 mem 13522.119 user 839.792 sys 782.766 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L006\tPU:L006\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.T18-D0003255_R1.fastq.gz 0004.T18-D0003255_R2.fastq.gz
[main] Real time: 782.813 sec; CPU: 14361.926 sec
sorting block 79, #reads = 1000887 mem = 497985522 peak = 1129656320
uniq header count 1
merging 80 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3307578790
output reads: 86308434
bam_mem_sort: 80 calls 34.679 user 0.613 sys 35.486 real
bam_write: 80 calls 20.250 user 1.141 sys 21.507 real
execute: 1 calls 16.394 user 0.838 sys 802.468 real
merge_files: 1 calls 16.365 user 0.819 sys 18.514 real
parse_chunk: 1279 calls 48.839 user 12.477 sys 62.383 real
read_chunk: 3443 calls 26.134 user 26.583 sys 410.543 real
sort_block: 1 calls 20.243 user 26.044 sys 401.467 real
write_chunk: 2162 calls 15.989 user 0.638 sys 17.586 real
overall: 2580512768 mem 718.897 user 50.457 sys 802.489 real
ls: cannot access '*.cram': No such file or directory