This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2501.623 CPU sec, 99.950 real sec
[M::process] read 676878 sequences (100000266 bp) in 1.238 CPU sec, 1.244 real sec...
[M::process] read 676484 sequences (100000201 bp) in 1.585 CPU sec, 1.619 real sec...
[M::process] read 676506 sequences (100000024 bp) in 1.590 CPU sec, 1.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288234, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.93, 108.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676240 sequences (100000094 bp) in 1.480 CPU sec, 1.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288266, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.52, 114.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288457, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.80, 112.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676878 reads in 300.990 CPU sec, 9.762 real sec
[M::mem_process_seqs] Processed 676506 reads in 185.627 CPU sec, 6.091 real sec
[M::mem_process_seqs] Processed 676484 reads in 192.783 CPU sec, 6.342 real sec
sorting block 0, #reads = 1081820 mem = 536878670 peak = 591929344
[M::process] read 675992 sequences (100000203 bp) in 1.951 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288109, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.52, 119.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676050 sequences (100000199 bp) in 1.354 CPU sec, 1.365 real sec...
[M::process] read 676188 sequences (100000216 bp) in 1.383 CPU sec, 1.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288567, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (243.78, 123.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676240 reads in 172.577 CPU sec, 5.492 real sec
[M::mem_process_seqs] Processed 675992 reads in 95.716 CPU sec, 3.045 real sec
sorting block 1, #reads = 1081727 mem = 536879341 peak = 871899136
sorting block 2, #reads = 1081900 mem = 536878027 peak = 871899136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287744, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.27, 123.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 892, 1660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4902)
[M::mem_pestat] mean and std.dev: (809.44, 816.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6523)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676586 sequences (100000043 bp) in 2.172 CPU sec, 2.306 real sec...
[M::process] read 676636 sequences (100000203 bp) in 1.411 CPU sec, 1.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287836, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (240.01, 120.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 33, 51)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 117)
[M::mem_pestat] mean and std.dev: (31.75, 21.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 150)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676050 reads in 186.003 CPU sec, 5.953 real sec
[M::mem_process_seqs] Processed 676188 reads in 104.079 CPU sec, 3.331 real sec
sorting block 3, #reads = 1081787 mem = 536878483 peak = 871899136
[M::process] read 676608 sequences (100000192 bp) in 1.600 CPU sec, 1.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288305, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.26, 112.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676398 sequences (100000166 bp) in 1.370 CPU sec, 1.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288125, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.86, 111.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288232, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.51, 114.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676586 reads in 276.929 CPU sec, 8.901 real sec
[M::mem_process_seqs] Processed 676636 reads in 196.320 CPU sec, 6.376 real sec
[M::mem_process_seqs] Processed 676608 reads in 205.817 CPU sec, 6.733 real sec
sorting block 4, #reads = 1081926 mem = 536876446 peak = 871899136
[M::process] read 676338 sequences (100000205 bp) in 1.921 CPU sec, 2.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288257, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.48, 114.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 5, #reads = 1082523 mem = 536879087 peak = 871899136
[M::process] read 676306 sequences (100000128 bp) in 2.087 CPU sec, 2.278 real sec...
[M::process] read 676610 sequences (100000260 bp) in 1.195 CPU sec, 1.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288187, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.77, 116.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 289, 572)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1542)
[M::mem_pestat] mean and std.dev: (317.35, 228.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2027)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676398 reads in 199.304 CPU sec, 6.444 real sec
[M::mem_process_seqs] Processed 676338 reads in 112.671 CPU sec, 3.629 real sec
sorting block 6, #reads = 1082310 mem = 536880729 peak = 871899136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288499, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.04, 116.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676590 sequences (100000064 bp) in 2.347 CPU sec, 2.748 real sec...
[M::process] read 676336 sequences (100000024 bp) in 1.348 CPU sec, 1.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288108, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.27, 111.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 38, 1248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3730)
[M::mem_pestat] mean and std.dev: (497.36, 548.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4971)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676306 reads in 195.544 CPU sec, 6.322 real sec
[M::mem_process_seqs] Processed 676610 reads in 111.390 CPU sec, 3.584 real sec
sorting block 7, #reads = 1082071 mem = 536879107 peak = 871899136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287979, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.60, 114.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676226 sequences (100000046 bp) in 2.045 CPU sec, 2.256 real sec...
[M::process] read 675978 sequences (100000209 bp) in 1.443 CPU sec, 1.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288060, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (236.26, 116.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 45, 965)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2839)
[M::mem_pestat] mean and std.dev: (291.91, 413.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3776)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676590 reads in 195.432 CPU sec, 6.290 real sec
[M::mem_process_seqs] Processed 676336 reads in 109.057 CPU sec, 3.502 real sec
sorting block 8, #reads = 1082252 mem = 536879494 peak = 871899136
sorting block 9, #reads = 1082203 mem = 536880819 peak = 875130880
[M::process] read 676072 sequences (100000265 bp) in 2.043 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287831, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (240.47, 120.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676210 sequences (100000245 bp) in 1.379 CPU sec, 1.383 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287752, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (245.75, 124.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287666, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.70, 123.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 2067, 2067)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5823)
[M::mem_pestat] mean and std.dev: (1279.00, 966.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7701)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676226 reads in 290.019 CPU sec, 9.357 real sec
[M::mem_process_seqs] Processed 675978 reads in 203.944 CPU sec, 6.582 real sec
[M::mem_process_seqs] Processed 676072 reads in 212.735 CPU sec, 6.911 real sec
sorting block 10, #reads = 1082286 mem = 536879231 peak = 875130880
[M::process] read 676888 sequences (100000067 bp) in 2.298 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287286, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (240.00, 120.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676636 sequences (100000085 bp) in 1.930 CPU sec, 2.077 real sec...
[M::process] read 676518 sequences (100000107 bp) in 1.484 CPU sec, 1.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288242, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.62, 107.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676210 reads in 203.901 CPU sec, 6.582 real sec
sorting block 11, #reads = 1082073 mem = 536879289 peak = 875130880
[M::mem_process_seqs] Processed 676888 reads in 113.097 CPU sec, 3.607 real sec
sorting block 12, #reads = 1081871 mem = 536878636 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287818, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.64, 112.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 34, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193)
[M::mem_pestat] mean and std.dev: (31.11, 27.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 255)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676598 sequences (100000171 bp) in 2.248 CPU sec, 2.499 real sec...
[M::process] read 676232 sequences (100000074 bp) in 1.498 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288094, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.11, 115.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676636 reads in 195.425 CPU sec, 6.291 real sec
[M::process] read 676580 sequences (100000066 bp) in 1.487 CPU sec, 1.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288308, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.32, 117.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 78, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (72.55, 80.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676598 reads in 106.005 CPU sec, 3.477 real sec
sorting block 13, #reads = 1082337 mem = 536880339 peak = 875130880
[M::mem_process_seqs] Processed 676518 reads in 252.917 CPU sec, 8.223 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287768, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (238.92, 119.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1082830 mem = 536880936 peak = 875130880
[M::process] read 676600 sequences (100000158 bp) in 1.951 CPU sec, 2.135 real sec...
[M::process] read 676686 sequences (100000048 bp) in 1.466 CPU sec, 1.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287981, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.79, 114.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 128, 169)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405)
[M::mem_pestat] mean and std.dev: (91.00, 55.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 523)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676232 reads in 199.696 CPU sec, 6.414 real sec
[M::mem_process_seqs] Processed 676580 reads in 107.570 CPU sec, 3.450 real sec
sorting block 15, #reads = 1081634 mem = 536879946 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288829, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.96, 111.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (332, 993, 1556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4004)
[M::mem_pestat] mean and std.dev: (1041.14, 1017.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5228)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676734 sequences (100000192 bp) in 2.246 CPU sec, 2.419 real sec...
[M::process] read 676326 sequences (100000223 bp) in 1.464 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287713, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.05, 110.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676600 reads in 188.445 CPU sec, 6.049 real sec
[M::mem_process_seqs] Processed 676686 reads in 105.761 CPU sec, 3.433 real sec
sorting block 16, #reads = 1082139 mem = 536879203 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288923, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.06, 113.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676152 sequences (100000247 bp) in 2.211 CPU sec, 2.420 real sec...
[M::process] read 676134 sequences (100000084 bp) in 1.535 CPU sec, 1.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288155, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.18, 117.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676734 reads in 191.720 CPU sec, 6.177 real sec
[M::mem_process_seqs] Processed 676326 reads in 106.926 CPU sec, 3.433 real sec
sorting block 17, #reads = 1082044 mem = 536877967 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287950, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.85, 120.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676470 sequences (100000152 bp) in 2.137 CPU sec, 2.297 real sec...
[M::process] read 676898 sequences (100000288 bp) in 1.530 CPU sec, 1.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287376, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.67, 120.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 713, 3273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9789)
[M::mem_pestat] mean and std.dev: (1243.18, 1357.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13047)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676152 reads in 197.288 CPU sec, 6.335 real sec
[M::mem_process_seqs] Processed 676134 reads in 110.634 CPU sec, 3.545 real sec
sorting block 18, #reads = 1083163 mem = 536879231 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288129, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.27, 115.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1081727 mem = 536878708 peak = 875130880
[M::process] read 676646 sequences (100000068 bp) in 2.036 CPU sec, 2.219 real sec...
[M::process] read 676398 sequences (100000114 bp) in 1.393 CPU sec, 1.410 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288085, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (228.17, 108.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 145, 2061)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6127)
[M::mem_pestat] mean and std.dev: (1195.83, 1652.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8160)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676470 reads in 195.157 CPU sec, 6.293 real sec
[M::process] read 676410 sequences (100000020 bp) in 1.555 CPU sec, 1.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288217, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.82, 115.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676646 reads in 106.286 CPU sec, 3.505 real sec
sorting block 20, #reads = 1082268 mem = 536877906 peak = 875130880
[M::mem_process_seqs] Processed 676898 reads in 250.422 CPU sec, 8.145 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287853, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.99, 116.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1082499 mem = 536880230 peak = 875130880
[M::process] read 676252 sequences (100000113 bp) in 2.123 CPU sec, 2.330 real sec...
[M::process] read 676966 sequences (100000269 bp) in 1.466 CPU sec, 1.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287702, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.42, 117.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 389, 2652)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7936)
[M::mem_pestat] mean and std.dev: (925.40, 1140.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10578)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676398 reads in 204.949 CPU sec, 6.580 real sec
[M::mem_process_seqs] Processed 676410 reads in 109.764 CPU sec, 3.531 real sec
sorting block 22, #reads = 1082560 mem = 536878273 peak = 875130880
[M::process] read 677122 sequences (100000023 bp) in 2.116 CPU sec, 2.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287474, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.99, 119.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676962 sequences (100000085 bp) in 1.559 CPU sec, 1.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288463, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.81, 108.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676252 reads in 195.546 CPU sec, 6.292 real sec
[M::mem_process_seqs] Processed 676966 reads in 108.848 CPU sec, 3.501 real sec
sorting block 23, #reads = 1081260 mem = 536878553 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288107, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.93, 105.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 41, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (155.00, 164.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1446)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676594 sequences (100000007 bp) in 1.932 CPU sec, 2.084 real sec...
[M::process] read 676552 sequences (100000206 bp) in 1.566 CPU sec, 1.568 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288130, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.19, 105.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677122 reads in 191.427 CPU sec, 6.157 real sec
[M::mem_process_seqs] Processed 676962 reads in 109.824 CPU sec, 3.521 real sec
sorting block 24, #reads = 1082494 mem = 536880774 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288310, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.94, 110.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676328 sequences (100000120 bp) in 2.047 CPU sec, 2.287 real sec...
[M::process] read 676474 sequences (100000159 bp) in 1.453 CPU sec, 1.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288388, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.98, 113.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676594 reads in 196.385 CPU sec, 6.339 real sec
[M::mem_process_seqs] Processed 676552 reads in 109.997 CPU sec, 3.535 real sec
sorting block 25, #reads = 1082345 mem = 536876847 peak = 875130880
sorting block 26, #reads = 1082786 mem = 536880245 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288179, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.57, 115.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (282, 380, 5188)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15000)
[M::mem_pestat] mean and std.dev: (2464.08, 3523.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19906)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676726 sequences (100000294 bp) in 2.082 CPU sec, 2.265 real sec...
[M::process] read 676764 sequences (100000016 bp) in 1.541 CPU sec, 1.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287780, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.90, 114.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676328 reads in 198.047 CPU sec, 6.377 real sec
[M::mem_process_seqs] Processed 676474 reads in 110.899 CPU sec, 3.549 real sec
sorting block 27, #reads = 1082498 mem = 536879053 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288210, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.33, 108.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676542 sequences (100000206 bp) in 2.255 CPU sec, 2.433 real sec...
[M::process] read 676348 sequences (100000078 bp) in 1.503 CPU sec, 1.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288331, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.35, 108.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676726 reads in 197.069 CPU sec, 6.348 real sec
[M::mem_process_seqs] Processed 676764 reads in 109.775 CPU sec, 3.521 real sec
sorting block 28, #reads = 1082539 mem = 536880686 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287713, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.98, 112.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 466, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1979)
[M::mem_pestat] mean and std.dev: (410.22, 410.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2628)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676240 sequences (100000216 bp) in 2.013 CPU sec, 2.211 real sec...
[M::process] read 676144 sequences (100000035 bp) in 1.559 CPU sec, 1.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287639, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.83, 115.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676542 reads in 199.168 CPU sec, 6.411 real sec
[M::mem_process_seqs] Processed 676348 reads in 111.319 CPU sec, 3.573 real sec
sorting block 29, #reads = 1082354 mem = 536876694 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287594, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.77, 119.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676972 sequences (100000242 bp) in 2.357 CPU sec, 2.519 real sec...
[M::process] read 676812 sequences (100000108 bp) in 1.557 CPU sec, 1.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288132, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (238.92, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 35, 51)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 139)
[M::mem_pestat] mean and std.dev: (27.22, 21.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 183)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676240 reads in 197.578 CPU sec, 6.350 real sec
[M::mem_process_seqs] Processed 676144 reads in 109.850 CPU sec, 3.519 real sec
sorting block 30, #reads = 1081855 mem = 536878564 peak = 875130880
sorting block 31, #reads = 1081815 mem = 536878955 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288284, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.22, 112.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676796 sequences (100000221 bp) in 2.170 CPU sec, 2.387 real sec...
[M::process] read 676516 sequences (100000044 bp) in 1.591 CPU sec, 1.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287676, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.27, 115.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 208, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1945)
[M::mem_pestat] mean and std.dev: (271.54, 280.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2590)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676972 reads in 197.081 CPU sec, 6.354 real sec
[M::mem_process_seqs] Processed 676812 reads in 110.864 CPU sec, 3.573 real sec
sorting block 32, #reads = 1082274 mem = 536880596 peak = 875130880
[M::process] read 675954 sequences (100000228 bp) in 2.074 CPU sec, 2.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287502, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.06, 116.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676156 sequences (100000130 bp) in 1.611 CPU sec, 1.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287223, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (242.60, 122.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287387, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.09, 117.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676796 reads in 294.035 CPU sec, 9.481 real sec
[M::mem_process_seqs] Processed 675954 reads in 205.751 CPU sec, 6.636 real sec
[M::mem_process_seqs] Processed 676516 reads in 214.237 CPU sec, 6.942 real sec
sorting block 33, #reads = 1082312 mem = 536879384 peak = 875130880
[M::process] read 676452 sequences (100000009 bp) in 2.489 CPU sec, 2.794 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287470, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (241.87, 122.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1081414 mem = 536880757 peak = 875130880
[M::process] read 676804 sequences (100000284 bp) in 2.150 CPU sec, 2.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287396, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.47, 120.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676156 reads in 207.176 CPU sec, 6.692 real sec
[M::process] read 676820 sequences (100000016 bp) in 1.514 CPU sec, 1.527 real sec...
[M::mem_process_seqs] Processed 676452 reads in 116.120 CPU sec, 3.702 real sec
sorting block 35, #reads = 1081746 mem = 536879561 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288264, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.85, 110.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676810 sequences (100000255 bp) in 2.165 CPU sec, 2.513 real sec...
[M::process] read 676616 sequences (100000135 bp) in 1.487 CPU sec, 1.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288228, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.67, 109.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676804 reads in 201.021 CPU sec, 6.498 real sec
[M::mem_process_seqs] Processed 676820 reads in 113.219 CPU sec, 3.630 real sec
sorting block 36, #reads = 1082253 mem = 536877004 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288206, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.17, 111.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676574 sequences (100000017 bp) in 2.597 CPU sec, 2.920 real sec...
[M::process] read 676816 sequences (100000063 bp) in 1.619 CPU sec, 1.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288144, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.63, 113.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676810 reads in 202.542 CPU sec, 6.533 real sec
[M::mem_process_seqs] Processed 676616 reads in 114.029 CPU sec, 3.656 real sec
sorting block 37, #reads = 1082170 mem = 536879112 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288061, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.57, 115.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1082559 mem = 536876195 peak = 875130880
[M::process] read 676800 sequences (100000296 bp) in 2.057 CPU sec, 2.368 real sec...
[M::process] read 676696 sequences (100000288 bp) in 1.617 CPU sec, 1.638 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287740, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.66, 113.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676574 reads in 198.053 CPU sec, 6.414 real sec
[M::mem_process_seqs] Processed 676816 reads in 111.351 CPU sec, 3.574 real sec
sorting block 39, #reads = 1081951 mem = 536877494 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287477, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.27, 115.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676796 sequences (100000121 bp) in 2.558 CPU sec, 2.860 real sec...
[M::process] read 676444 sequences (100000189 bp) in 1.627 CPU sec, 1.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288059, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.19, 115.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676800 reads in 202.774 CPU sec, 6.524 real sec
[M::mem_process_seqs] Processed 676696 reads in 112.697 CPU sec, 3.635 real sec
sorting block 40, #reads = 1081661 mem = 536877077 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287890, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.35, 115.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676210 sequences (100000131 bp) in 2.496 CPU sec, 2.773 real sec...
[M::process] read 676258 sequences (100000072 bp) in 2.103 CPU sec, 2.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286899, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.51, 119.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676796 reads in 209.106 CPU sec, 6.719 real sec
[M::mem_process_seqs] Processed 676444 reads in 117.581 CPU sec, 3.755 real sec
sorting block 41, #reads = 1082550 mem = 536877595 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286919, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (243.58, 124.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 49, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187)
[M::mem_pestat] mean and std.dev: (40.80, 24.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 244)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676798 sequences (100000105 bp) in 2.425 CPU sec, 2.744 real sec...
[M::process] read 677138 sequences (100000094 bp) in 1.626 CPU sec, 1.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286850, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.07, 120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 58, 58)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 98)
[M::mem_pestat] mean and std.dev: (44.80, 17.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 118)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676210 reads in 213.748 CPU sec, 6.884 real sec
[M::mem_process_seqs] Processed 676258 reads in 119.585 CPU sec, 3.833 real sec
sorting block 42, #reads = 1081677 mem = 536878842 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287631, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.92, 112.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 43, #reads = 1081601 mem = 536878970 peak = 875130880
[M::process] read 676980 sequences (100000107 bp) in 2.464 CPU sec, 2.961 real sec...
[M::process] read 676806 sequences (100000264 bp) in 1.570 CPU sec, 1.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288319, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.41, 103.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676798 reads in 207.739 CPU sec, 6.737 real sec
[M::mem_process_seqs] Processed 677138 reads in 115.504 CPU sec, 3.723 real sec
sorting block 44, #reads = 1082389 mem = 536878626 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287472, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.15, 109.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 91, 91)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255)
[M::mem_pestat] mean and std.dev: (53.00, 37.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 337)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676658 sequences (100000279 bp) in 2.325 CPU sec, 2.698 real sec...
[M::process] read 676814 sequences (100000278 bp) in 1.538 CPU sec, 1.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288260, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.61, 112.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676980 reads in 207.567 CPU sec, 6.747 real sec
[M::mem_process_seqs] Processed 676806 reads in 115.392 CPU sec, 3.753 real sec
sorting block 45, #reads = 1082194 mem = 536883476 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287868, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.01, 115.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676978 sequences (100000086 bp) in 2.499 CPU sec, 2.705 real sec...
[M::process] read 677004 sequences (100000112 bp) in 1.578 CPU sec, 1.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287378, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.04, 111.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676658 reads in 206.009 CPU sec, 6.649 real sec
[M::mem_process_seqs] Processed 676814 reads in 112.876 CPU sec, 3.625 real sec
sorting block 46, #reads = 1082937 mem = 536876923 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287769, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.15, 111.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676880 sequences (100000028 bp) in 2.564 CPU sec, 3.027 real sec...
[M::process] read 676780 sequences (100000277 bp) in 1.418 CPU sec, 1.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287567, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.18, 110.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676978 reads in 206.427 CPU sec, 6.686 real sec
[M::mem_process_seqs] Processed 677004 reads in 115.555 CPU sec, 3.716 real sec
sorting block 47, #reads = 1081426 mem = 536877329 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287644, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.11, 109.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1082374 mem = 536877099 peak = 875130880
[M::process] read 676632 sequences (100000283 bp) in 2.405 CPU sec, 2.736 real sec...
[M::process] read 676334 sequences (100000039 bp) in 1.527 CPU sec, 1.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287526, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.36, 111.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676880 reads in 203.041 CPU sec, 6.574 real sec
[M::mem_process_seqs] Processed 676780 reads in 112.038 CPU sec, 3.619 real sec
sorting block 49, #reads = 1082251 mem = 536879081 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287056, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.14, 117.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 51, 856)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2536)
[M::mem_pestat] mean and std.dev: (423.29, 502.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3376)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676478 sequences (100000057 bp) in 2.438 CPU sec, 2.699 real sec...
[M::process] read 677072 sequences (100000105 bp) in 1.606 CPU sec, 1.616 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287010, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.58, 120.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 12, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (81.17, 118.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676632 reads in 210.149 CPU sec, 6.804 real sec
[M::mem_process_seqs] Processed 676334 reads in 117.101 CPU sec, 3.807 real sec
sorting block 50, #reads = 1081769 mem = 536879381 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287150, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.55, 116.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676988 sequences (100000022 bp) in 2.458 CPU sec, 2.692 real sec...
[M::process] read 676938 sequences (100000096 bp) in 1.563 CPU sec, 1.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287821, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.23, 101.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676478 reads in 209.068 CPU sec, 6.738 real sec
[M::mem_process_seqs] Processed 677072 reads in 113.970 CPU sec, 3.652 real sec
sorting block 51, #reads = 1081845 mem = 536881146 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288159, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.62, 110.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676756 sequences (100000159 bp) in 2.523 CPU sec, 2.826 real sec...
[M::process] read 676654 sequences (100000157 bp) in 1.627 CPU sec, 1.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288041, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.76, 112.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 78, 3555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10509)
[M::mem_pestat] mean and std.dev: (1051.58, 1489.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13986)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676988 reads in 207.554 CPU sec, 6.676 real sec
[M::mem_process_seqs] Processed 676938 reads in 116.163 CPU sec, 3.727 real sec
sorting block 52, #reads = 1082378 mem = 536880520 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287983, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.45, 113.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677188 sequences (100000205 bp) in 2.599 CPU sec, 2.984 real sec...
sorting block 53, #reads = 1082361 mem = 536880286 peak = 875130880
[M::process] read 677366 sequences (100000004 bp) in 1.462 CPU sec, 1.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287952, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.12, 114.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676756 reads in 203.637 CPU sec, 6.583 real sec
[M::mem_process_seqs] Processed 676654 reads in 113.136 CPU sec, 3.627 real sec
sorting block 54, #reads = 1082142 mem = 536883836 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287832, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.13, 109.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677350 sequences (100000021 bp) in 2.395 CPU sec, 2.675 real sec...
[M::process] read 676972 sequences (100000181 bp) in 1.657 CPU sec, 1.664 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288458, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.64, 107.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 336, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1380)
[M::mem_pestat] mean and std.dev: (255.33, 217.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1823)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677188 reads in 198.824 CPU sec, 6.407 real sec
[M::mem_process_seqs] Processed 677366 reads in 112.368 CPU sec, 3.600 real sec
sorting block 55, #reads = 1081237 mem = 536878015 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287634, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.99, 106.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676758 sequences (100000111 bp) in 2.379 CPU sec, 2.611 real sec...
[M::process] read 676692 sequences (100000143 bp) in 1.497 CPU sec, 1.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287955, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.76, 108.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 66, 702)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2048)
[M::mem_pestat] mean and std.dev: (311.83, 400.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2721)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677350 reads in 207.595 CPU sec, 6.765 real sec
[M::mem_process_seqs] Processed 676972 reads in 117.056 CPU sec, 3.817 real sec
sorting block 56, #reads = 1082001 mem = 536879902 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287549, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.65, 112.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676746 sequences (100000029 bp) in 3.550 CPU sec, 4.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287598, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.65, 114.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676758 reads in 210.330 CPU sec, 6.742 real sec
[M::mem_process_seqs] Processed 676692 reads in 118.140 CPU sec, 3.740 real sec
sorting block 57, #reads = 1081737 mem = 536880647 peak = 875130880
[M::process] read 677082 sequences (100000068 bp) in 3.762 CPU sec, 4.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287343, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.84, 114.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1082242 mem = 536878887 peak = 875130880
[M::process] read 677050 sequences (100000110 bp) in 2.868 CPU sec, 3.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287749, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.25, 107.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 35, 46)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 68)
[M::mem_pestat] mean and std.dev: (38.78, 9.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 79)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676746 reads in 209.328 CPU sec, 6.741 real sec
[M::mem_process_seqs] Processed 677082 reads in 116.656 CPU sec, 3.727 real sec
[M::process] read 676762 sequences (100000045 bp) in 1.740 CPU sec, 1.806 real sec...
sorting block 59, #reads = 1082021 mem = 536880671 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287962, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.02, 108.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676662 sequences (100000000 bp) in 2.798 CPU sec, 3.051 real sec...
[M::process] read 676496 sequences (100000076 bp) in 1.474 CPU sec, 1.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287888, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.08, 112.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677050 reads in 204.279 CPU sec, 6.550 real sec
[M::mem_process_seqs] Processed 676762 reads in 115.000 CPU sec, 3.674 real sec
sorting block 60, #reads = 1081807 mem = 536878414 peak = 875130880
[M::process] read 552760 sequences (81707875 bp) in 2.314 CPU sec, 2.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287457, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.62, 114.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287644, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.93, 117.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676662 reads in 207.406 CPU sec, 6.706 real sec
[M::mem_process_seqs] Processed 676496 reads in 114.836 CPU sec, 3.734 real sec
sorting block 61, #reads = 1082575 mem = 536877494 peak = 875130880
sorting block 62, #reads = 1081977 mem = 536878010 peak = 875130880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 234588, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.51, 116.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 79, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183)
[M::mem_pestat] mean and std.dev: (49.33, 30.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 231)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 552760 reads in 89.765 CPU sec, 3.021 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 68214026
overall: 69360373760 mem 12364.794 user 652.110 sys 451.080 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L004\tPU:L004\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.T21-D0003376_R1.fastq.gz 0001.T21-D0003376_R2.fastq.gz
[main] Real time: 451.111 sec; CPU: 13016.908 sec
sorting block 63, #reads = 564488 mem = 280177216 peak = 875130880
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2648685031
output reads: 68738215
bam_mem_sort: 64 calls 56.554 user 1.026 sys 58.803 real
bam_write: 64 calls 43.699 user 4.129 sys 79.336 real
execute: 1 calls 27.786 user 2.976 sys 483.848 real
merge_files: 1 calls 27.755 user 2.947 sys 31.962 real
parse_chunk: 1017 calls 48.496 user 14.461 sys 74.384 real
read_chunk: 2738 calls 32.745 user 15.459 sys 352.830 real
sort_block: 1 calls 24.396 user 14.419 sys 340.888 real
write_chunk: 1719 calls 27.247 user 2.587 sys 30.792 real
overall: 2014552064 mem 815.710 user 69.775 sys 483.862 real
ls: cannot access '*.cram': No such file or directory