Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/55/698ce90980b6dfdc5d35f0cd11dc5e/T21-D0003322/T21-D0003322_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b4/c7a15a0dae1c85118e496614b1debf/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/55/698ce90980b6dfdc5d35f0cd11dc5e/T21-D0003322/T21-D0003322_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b4/c7a15a0dae1c85118e496614b1debf/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003322.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1892.807 CPU sec, 69.188 real sec
[M::process] read 675870 sequences (100000025 bp) in 1.258 CPU sec, 1.259 real sec...
[M::process] read 675816 sequences (100000137 bp) in 1.917 CPU sec, 2.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286628, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (238.43, 113.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675944 sequences (100000021 bp) in 1.779 CPU sec, 1.860 real sec...
[M::process] read 675734 sequences (100000129 bp) in 1.449 CPU sec, 1.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286930, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (239.26, 114.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675870 reads in 225.569 CPU sec, 7.404 real sec
[M::mem_process_seqs] Processed 675816 reads in 123.622 CPU sec, 4.118 real sec
sorting block 0, #reads = 1081185 mem = 536879477 peak = 621387776
[M::process] read 675690 sequences (100000138 bp) in 2.148 CPU sec, 2.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286706, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.54, 115.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 53, 892)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2642)
[M::mem_pestat] mean and std.dev: (413.77, 634.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3517)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675608 sequences (100000171 bp) in 1.849 CPU sec, 1.877 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286634, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (242.13, 116.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286781, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.39, 116.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675944 reads in 315.798 CPU sec, 10.178 real sec
[M::mem_process_seqs] Processed 675690 reads in 218.663 CPU sec, 7.124 real sec
[M::mem_process_seqs] Processed 675734 reads in 228.124 CPU sec, 7.446 real sec
sorting block 1, #reads = 1080749 mem = 536878960 peak = 749576192
sorting block 2, #reads = 1081484 mem = 536877169 peak = 982097920
[M::process] read 675660 sequences (100000269 bp) in 2.580 CPU sec, 2.608 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286447, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.51, 117.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675532 sequences (100000229 bp) in 1.890 CPU sec, 1.887 real sec...
[M::process] read 675556 sequences (100000106 bp) in 1.890 CPU sec, 1.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286401, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.26, 118.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675608 reads in 220.248 CPU sec, 6.974 real sec
[M::mem_process_seqs] Processed 675660 reads in 120.936 CPU sec, 3.818 real sec
sorting block 3, #reads = 1080644 mem = 536879386 peak = 982097920
[M::process] read 675584 sequences (100000165 bp) in 2.235 CPU sec, 2.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286810, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (244.00, 118.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675440 sequences (100000130 bp) in 1.880 CPU sec, 1.877 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286295, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.29, 120.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 76, 184)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (103.83, 100.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 667)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286769, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.23, 119.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 238, 1216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3240)
[M::mem_pestat] mean and std.dev: (734.20, 657.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4252)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675532 reads in 316.620 CPU sec, 10.133 real sec
[M::mem_process_seqs] Processed 675584 reads in 219.337 CPU sec, 7.094 real sec
[M::mem_process_seqs] Processed 675556 reads in 228.909 CPU sec, 7.424 real sec
sorting block 4, #reads = 1081025 mem = 536878599 peak = 982097920
sorting block 5, #reads = 1080545 mem = 536880035 peak = 982097920
[M::process] read 675512 sequences (100000237 bp) in 2.147 CPU sec, 2.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286625, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (245.85, 120.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675504 sequences (100000072 bp) in 1.632 CPU sec, 1.635 real sec...
[M::process] read 675318 sequences (100000220 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286419, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (246.13, 120.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 663, 1427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4237)
[M::mem_pestat] mean and std.dev: (639.73, 663.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5642)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675440 reads in 223.909 CPU sec, 7.136 real sec
[M::mem_process_seqs] Processed 675512 reads in 125.496 CPU sec, 3.998 real sec
sorting block 6, #reads = 1080927 mem = 536879597 peak = 982097920
[M::process] read 675352 sequences (100000027 bp) in 2.167 CPU sec, 2.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286031, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (245.94, 120.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 663, 663)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1963)
[M::mem_pestat] mean and std.dev: (411.10, 306.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2613)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675268 sequences (100000004 bp) in 1.870 CPU sec, 1.865 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285803, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (247.13, 120.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 61, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181)
[M::mem_pestat] mean and std.dev: (170.80, 222.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1572)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675504 reads in 231.196 CPU sec, 7.378 real sec
[M::mem_process_seqs] Processed 675318 reads in 127.223 CPU sec, 4.076 real sec
sorting block 7, #reads = 1081053 mem = 536882059 peak = 993988608
[M::process] read 675404 sequences (100000226 bp) in 1.996 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286046, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.83, 122.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675266 sequences (100000205 bp) in 1.430 CPU sec, 1.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286476, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.82, 122.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286175, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.45, 122.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675352 reads in 329.492 CPU sec, 10.559 real sec
[M::mem_process_seqs] Processed 675404 reads in 226.953 CPU sec, 7.325 real sec
[M::mem_process_seqs] Processed 675268 reads in 235.476 CPU sec, 7.621 real sec
sorting block 8, #reads = 1080870 mem = 536878816 peak = 1004642304
sorting block 9, #reads = 1080797 mem = 536881995 peak = 1004642304
[M::process] read 675242 sequences (100000058 bp) in 2.106 CPU sec, 2.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286173, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.63, 120.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675062 sequences (100000233 bp) in 1.656 CPU sec, 1.660 real sec...
[M::process] read 675108 sequences (100000298 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285254, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.87, 125.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 319, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (276.30, 250.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675266 reads in 233.402 CPU sec, 7.443 real sec
[M::mem_process_seqs] Processed 675242 reads in 129.417 CPU sec, 4.133 real sec
sorting block 10, #reads = 1081316 mem = 536879327 peak = 1057345536
[M::process] read 675032 sequences (100000136 bp) in 2.413 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286163, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.38, 125.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 32, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772)
[M::mem_pestat] mean and std.dev: (249.80, 288.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2360)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675016 sequences (100000281 bp) in 1.940 CPU sec, 1.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285443, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (254.13, 127.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 10, 17)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 33)
[M::mem_pestat] mean and std.dev: (12.45, 7.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 41)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285075, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.90, 125.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675062 reads in 340.522 CPU sec, 10.897 real sec
sorting block 11, #reads = 1080364 mem = 536878325 peak = 1057484800
[M::mem_process_seqs] Processed 675108 reads in 237.824 CPU sec, 7.658 real sec
[M::mem_process_seqs] Processed 675032 reads in 247.608 CPU sec, 7.990 real sec
sorting block 12, #reads = 1081062 mem = 536879206 peak = 1065172992
[M::process] read 675124 sequences (100000034 bp) in 2.614 CPU sec, 2.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285888, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (252.77, 126.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674968 sequences (100000229 bp) in 2.100 CPU sec, 2.092 real sec...
[M::process] read 675074 sequences (100000149 bp) in 1.960 CPU sec, 1.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285947, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.73, 126.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 23, 32)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 80)
[M::mem_pestat] mean and std.dev: (17.24, 10.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 104)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675016 reads in 239.222 CPU sec, 7.576 real sec
sorting block 13, #reads = 1080296 mem = 536878117 peak = 1076641792
[M::mem_process_seqs] Processed 675124 reads in 130.664 CPU sec, 4.124 real sec
sorting block 14, #reads = 1080964 mem = 536876598 peak = 1085599744
[M::process] read 675020 sequences (100000085 bp) in 2.425 CPU sec, 2.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285578, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.89, 126.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 196, 799)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2333)
[M::mem_pestat] mean and std.dev: (342.08, 373.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3100)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675030 sequences (100000100 bp) in 1.959 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285849, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.36, 127.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285595, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (253.30, 126.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674968 reads in 345.606 CPU sec, 11.076 real sec
[M::mem_process_seqs] Processed 675074 reads in 237.809 CPU sec, 7.661 real sec
[M::mem_process_seqs] Processed 675020 reads in 247.001 CPU sec, 7.974 real sec
sorting block 15, #reads = 1080832 mem = 536879887 peak = 1085599744
sorting block 16, #reads = 1080101 mem = 536881516 peak = 1112702976
[M::process] read 674990 sequences (100000287 bp) in 2.926 CPU sec, 3.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285875, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.98, 127.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674876 sequences (100000187 bp) in 1.910 CPU sec, 1.914 real sec...
[M::process] read 674626 sequences (100000068 bp) in 1.460 CPU sec, 1.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285740, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (254.03, 127.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (239, 261, 999)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2519)
[M::mem_pestat] mean and std.dev: (501.47, 545.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3279)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675030 reads in 242.402 CPU sec, 7.704 real sec
[M::mem_process_seqs] Processed 674990 reads in 134.492 CPU sec, 4.269 real sec
sorting block 17, #reads = 1080592 mem = 536880250 peak = 1112702976
[M::process] read 674780 sequences (100000271 bp) in 1.992 CPU sec, 2.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285426, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (255.27, 128.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674854 sequences (100000203 bp) in 2.000 CPU sec, 2.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285095, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.03, 130.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284902, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (254.79, 127.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674876 reads in 340.881 CPU sec, 10.897 real sec
[M::mem_process_seqs] Processed 674626 reads in 234.149 CPU sec, 7.538 real sec
[M::mem_process_seqs] Processed 674780 reads in 243.733 CPU sec, 7.861 real sec
sorting block 18, #reads = 1080634 mem = 536880650 peak = 1112702976
sorting block 19, #reads = 1080715 mem = 536880602 peak = 1118380032
[M::process] read 674700 sequences (100000267 bp) in 2.743 CPU sec, 2.861 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285185, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.86, 128.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674860 sequences (100000132 bp) in 2.030 CPU sec, 2.031 real sec...
[M::process] read 674752 sequences (100000123 bp) in 1.980 CPU sec, 1.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285548, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.88, 129.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 173, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1946)
[M::mem_pestat] mean and std.dev: (261.46, 272.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2591)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674854 reads in 234.526 CPU sec, 7.400 real sec
[M::mem_process_seqs] Processed 674700 reads in 127.548 CPU sec, 4.016 real sec
sorting block 20, #reads = 1080904 mem = 536878450 peak = 1118380032
[M::process] read 674824 sequences (100000160 bp) in 2.441 CPU sec, 2.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284983, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.34, 130.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 23, 2067)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6199)
[M::mem_pestat] mean and std.dev: (563.13, 907.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8265)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674726 sequences (100000087 bp) in 1.970 CPU sec, 1.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285197, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.30, 131.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285034, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.40, 132.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674860 reads in 341.380 CPU sec, 10.902 real sec
[M::mem_process_seqs] Processed 674752 reads in 236.399 CPU sec, 7.583 real sec
sorting block 21, #reads = 1080145 mem = 536880135 peak = 1118380032
[M::mem_process_seqs] Processed 674824 reads in 246.347 CPU sec, 7.998 real sec
sorting block 22, #reads = 1080205 mem = 536880680 peak = 1118380032
[M::process] read 674764 sequences (100000013 bp) in 2.395 CPU sec, 2.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285694, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.91, 132.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674812 sequences (100000266 bp) in 2.119 CPU sec, 2.125 real sec...
[M::process] read 674760 sequences (100000198 bp) in 2.000 CPU sec, 1.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285452, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.48, 132.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 20, 32)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 92)
[M::mem_pestat] mean and std.dev: (16.18, 13.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 122)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674726 reads in 235.607 CPU sec, 7.455 real sec
[M::mem_process_seqs] Processed 674764 reads in 129.339 CPU sec, 4.079 real sec
sorting block 23, #reads = 1080269 mem = 536878960 peak = 1118380032
[M::process] read 674700 sequences (100000234 bp) in 2.615 CPU sec, 2.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285796, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.67, 132.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 1500, 1500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4154)
[M::mem_pestat] mean and std.dev: (887.67, 665.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5481)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674634 sequences (100000211 bp) in 1.970 CPU sec, 1.969 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285214, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.07, 134.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285217, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.40, 133.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674812 reads in 332.146 CPU sec, 10.597 real sec
[M::mem_process_seqs] Processed 674700 reads in 231.939 CPU sec, 7.446 real sec
sorting block 24, #reads = 1080687 mem = 536879642 peak = 1124843520
[M::mem_process_seqs] Processed 674760 reads in 241.719 CPU sec, 7.778 real sec
sorting block 25, #reads = 1079971 mem = 536879590 peak = 1128861696
[M::process] read 674508 sequences (100000230 bp) in 2.848 CPU sec, 3.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285504, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.26, 135.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674578 sequences (100000035 bp) in 2.040 CPU sec, 2.036 real sec...
[M::process] read 674592 sequences (100000279 bp) in 2.000 CPU sec, 2.003 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285558, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.30, 136.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 58, 58)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 124)
[M::mem_pestat] mean and std.dev: (44.80, 16.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 157)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674634 reads in 234.091 CPU sec, 7.414 real sec
[M::mem_process_seqs] Processed 674508 reads in 126.542 CPU sec, 3.976 real sec
sorting block 26, #reads = 1080467 mem = 536879220 peak = 1128861696
[M::process] read 674636 sequences (100000062 bp) in 2.491 CPU sec, 2.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285916, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.85, 137.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 426, 1029)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3001)
[M::mem_pestat] mean and std.dev: (418.50, 425.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3987)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674990 sequences (100000011 bp) in 2.000 CPU sec, 2.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285768, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.95, 138.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285844, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.01, 136.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 73, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (101.73, 87.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 768)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674578 reads in 330.870 CPU sec, 10.559 real sec
[M::mem_process_seqs] Processed 674636 reads in 228.699 CPU sec, 7.338 real sec
sorting block 27, #reads = 1080311 mem = 536879503 peak = 1142841344
[M::mem_process_seqs] Processed 674592 reads in 244.903 CPU sec, 7.880 real sec
sorting block 28, #reads = 1080416 mem = 536882680 peak = 1169539072
[M::process] read 675872 sequences (100000166 bp) in 2.922 CPU sec, 3.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285914, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.39, 130.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675812 sequences (100000185 bp) in 2.080 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286607, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (240.41, 116.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674990 reads in 218.243 CPU sec, 6.911 real sec
[M::mem_process_seqs] Processed 675872 reads in 121.086 CPU sec, 3.786 real sec
[M::process] read 675828 sequences (100000255 bp) in 2.085 CPU sec, 2.089 real sec...
sorting block 29, #reads = 1079371 mem = 536878055 peak = 1169539072
[M::process] read 675570 sequences (100000138 bp) in 2.432 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286296, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (240.90, 116.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 58, 680)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1924)
[M::mem_pestat] mean and std.dev: (396.11, 588.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2750)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675568 sequences (100000264 bp) in 1.480 CPU sec, 1.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286373, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.30, 116.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286878, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.71, 116.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675812 reads in 315.972 CPU sec, 10.106 real sec
sorting block 30, #reads = 1080713 mem = 536878905 peak = 1169539072
[M::mem_process_seqs] Processed 675570 reads in 217.434 CPU sec, 6.981 real sec
[M::mem_process_seqs] Processed 675828 reads in 226.522 CPU sec, 7.297 real sec
sorting block 31, #reads = 1080784 mem = 536877465 peak = 1174966272
[M::process] read 675730 sequences (100000254 bp) in 2.308 CPU sec, 2.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286958, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.46, 119.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675606 sequences (100000219 bp) in 1.812 CPU sec, 1.816 real sec...
[M::process] read 675478 sequences (100000092 bp) in 1.490 CPU sec, 1.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286586, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.21, 119.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675568 reads in 221.715 CPU sec, 7.071 real sec
sorting block 32, #reads = 1081258 mem = 536881344 peak = 1174966272
[M::mem_process_seqs] Processed 675730 reads in 123.270 CPU sec, 3.906 real sec
sorting block 33, #reads = 1080675 mem = 536879680 peak = 1174966272
[M::process] read 675610 sequences (100000157 bp) in 2.785 CPU sec, 2.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286503, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.39, 119.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 22, 714)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2136)
[M::mem_pestat] mean and std.dev: (309.07, 350.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2847)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675544 sequences (100000291 bp) in 1.460 CPU sec, 1.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286316, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.68, 120.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 23, 32)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 74)
[M::mem_pestat] mean and std.dev: (20.75, 8.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 95)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286289, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.07, 119.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 104, 2670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7872)
[M::mem_pestat] mean and std.dev: (1012.55, 1253.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10473)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675606 reads in 321.357 CPU sec, 10.275 real sec
[M::mem_process_seqs] Processed 675478 reads in 222.028 CPU sec, 7.122 real sec
[M::mem_process_seqs] Processed 675610 reads in 231.595 CPU sec, 7.444 real sec
sorting block 34, #reads = 1081452 mem = 536878130 peak = 1174966272
[M::process] read 675578 sequences (100000108 bp) in 2.927 CPU sec, 2.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286099, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (245.81, 120.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675466 sequences (100000013 bp) in 2.110 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286167, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.29, 120.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675286 sequences (100000095 bp) in 1.980 CPU sec, 1.982 real sec...
[M::mem_process_seqs] Processed 675544 reads in 226.366 CPU sec, 7.146 real sec
sorting block 35, #reads = 1080729 mem = 536879211 peak = 1192407040
[M::mem_process_seqs] Processed 675578 reads in 127.847 CPU sec, 4.010 real sec
sorting block 36, #reads = 1080656 mem = 536878332 peak = 1192407040
[M::process] read 675302 sequences (100000058 bp) in 1.962 CPU sec, 2.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285818, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.28, 122.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 386, 667)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1965)
[M::mem_pestat] mean and std.dev: (331.38, 348.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2614)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675396 sequences (100000077 bp) in 1.421 CPU sec, 1.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286440, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.60, 123.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286062, 15, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.59, 122.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 9, 15)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 41)
[M::mem_pestat] mean and std.dev: (6.33, 4.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 54)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675466 reads in 325.656 CPU sec, 10.435 real sec
[M::mem_process_seqs] Processed 675286 reads in 224.007 CPU sec, 7.220 real sec
[M::mem_process_seqs] Processed 675302 reads in 233.597 CPU sec, 7.544 real sec
sorting block 37, #reads = 1080968 mem = 536879519 peak = 1192407040
[M::process] read 675328 sequences (100000197 bp) in 2.348 CPU sec, 2.416 real sec...
sorting block 38, #reads = 1081262 mem = 536878049 peak = 1192407040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286474, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.15, 122.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675260 sequences (100000064 bp) in 2.160 CPU sec, 2.268 real sec...
[M::process] read 675080 sequences (100000040 bp) in 2.000 CPU sec, 2.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286508, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.40, 123.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675396 reads in 221.922 CPU sec, 7.025 real sec
[M::mem_process_seqs] Processed 675328 reads in 124.210 CPU sec, 3.908 real sec
sorting block 39, #reads = 1081292 mem = 536879912 peak = 1192407040
[M::process] read 675146 sequences (100000102 bp) in 2.598 CPU sec, 2.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285953, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.22, 123.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675142 sequences (100000282 bp) in 1.980 CPU sec, 1.975 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285059, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.95, 126.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 190, 190)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (109.11, 76.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286002, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.29, 125.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 419, 745)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2171)
[M::mem_pestat] mean and std.dev: (532.65, 594.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2911)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675260 reads in 320.777 CPU sec, 10.229 real sec
[M::mem_process_seqs] Processed 675080 reads in 223.585 CPU sec, 7.170 real sec
[M::mem_process_seqs] Processed 675146 reads in 233.217 CPU sec, 7.498 real sec
sorting block 40, #reads = 1080793 mem = 536879222 peak = 1192407040
sorting block 41, #reads = 1080118 mem = 536879325 peak = 1192407040
[M::process] read 675090 sequences (100000173 bp) in 2.535 CPU sec, 2.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286085, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.07, 128.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 11, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596)
[M::mem_pestat] mean and std.dev: (74.00, 89.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675134 sequences (100000159 bp) in 2.148 CPU sec, 2.155 real sec...
[M::process] read 675056 sequences (100000183 bp) in 2.000 CPU sec, 2.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285930, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.95, 127.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 88, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1760)
[M::mem_pestat] mean and std.dev: (240.90, 259.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2342)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675142 reads in 227.968 CPU sec, 7.209 real sec
[M::mem_process_seqs] Processed 675090 reads in 129.683 CPU sec, 4.095 real sec
sorting block 42, #reads = 1080560 mem = 536879552 peak = 1192407040
[M::process] read 675050 sequences (100000132 bp) in 2.434 CPU sec, 2.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285729, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.44, 127.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675020 sequences (100000105 bp) in 1.980 CPU sec, 1.978 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285826, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.99, 127.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286512, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.56, 129.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675134 reads in 327.707 CPU sec, 10.457 real sec
[M::mem_process_seqs] Processed 675050 reads in 226.179 CPU sec, 7.260 real sec
[M::mem_process_seqs] Processed 675056 reads in 235.751 CPU sec, 7.581 real sec
sorting block 43, #reads = 1080678 mem = 536878274 peak = 1192407040
sorting block 44, #reads = 1080783 mem = 536880347 peak = 1192407040
[M::process] read 674954 sequences (100000052 bp) in 2.517 CPU sec, 2.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285817, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.95, 127.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674948 sequences (100000050 bp) in 2.127 CPU sec, 2.128 real sec...
[M::process] read 674858 sequences (100000206 bp) in 1.990 CPU sec, 1.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285548, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (255.08, 128.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675020 reads in 227.072 CPU sec, 7.200 real sec
[M::mem_process_seqs] Processed 674954 reads in 125.384 CPU sec, 3.943 real sec
sorting block 45, #reads = 1080546 mem = 536882113 peak = 1192407040
[M::process] read 674930 sequences (100000249 bp) in 2.612 CPU sec, 2.696 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285578, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.55, 127.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674854 sequences (100000221 bp) in 1.970 CPU sec, 1.966 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285698, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.80, 128.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 225, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (203.62, 144.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1337)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285992, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.97, 128.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674948 reads in 325.921 CPU sec, 10.394 real sec
[M::mem_process_seqs] Processed 674858 reads in 227.820 CPU sec, 7.305 real sec
[M::mem_process_seqs] Processed 674930 reads in 237.624 CPU sec, 7.637 real sec
sorting block 46, #reads = 1080566 mem = 536879546 peak = 1201233920
sorting block 47, #reads = 1080173 mem = 536880462 peak = 1220005888
[M::process] read 674894 sequences (100000238 bp) in 2.749 CPU sec, 2.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285734, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.69, 128.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674750 sequences (100000228 bp) in 2.058 CPU sec, 2.065 real sec...
[M::process] read 674632 sequences (100000199 bp) in 1.980 CPU sec, 1.981 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285682, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.83, 128.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674854 reads in 229.848 CPU sec, 7.326 real sec
[M::mem_process_seqs] Processed 674894 reads in 125.311 CPU sec, 3.952 real sec
sorting block 48, #reads = 1080442 mem = 536877635 peak = 1220005888
[M::process] read 674788 sequences (100000081 bp) in 2.487 CPU sec, 2.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285790, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.76, 128.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 254, 749)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2221)
[M::mem_pestat] mean and std.dev: (480.10, 586.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2957)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674954 sequences (100000153 bp) in 1.960 CPU sec, 1.958 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286013, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.77, 129.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285573, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.31, 129.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674750 reads in 332.095 CPU sec, 10.623 real sec
[M::mem_process_seqs] Processed 674632 reads in 228.088 CPU sec, 7.317 real sec
sorting block 49, #reads = 1080444 mem = 536881346 peak = 1222533120
[M::mem_process_seqs] Processed 674788 reads in 237.944 CPU sec, 7.650 real sec
sorting block 50, #reads = 1080386 mem = 536877621 peak = 1222983680
[M::process] read 674718 sequences (100000235 bp) in 2.518 CPU sec, 2.626 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285927, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.72, 130.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674752 sequences (100000038 bp) in 1.510 CPU sec, 1.512 real sec...
[M::process] read 674800 sequences (100000020 bp) in 1.470 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285387, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.47, 131.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674954 reads in 232.619 CPU sec, 7.410 real sec
[M::mem_process_seqs] Processed 674718 reads in 125.188 CPU sec, 3.988 real sec
sorting block 51, #reads = 1080471 mem = 536880764 peak = 1231159296
[M::process] read 674880 sequences (100000243 bp) in 2.480 CPU sec, 2.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285354, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.14, 131.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 494, 2742)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8196)
[M::mem_pestat] mean and std.dev: (944.30, 1189.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10923)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674864 sequences (100000273 bp) in 1.980 CPU sec, 1.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285632, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.95, 131.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285627, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.48, 132.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 81, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (107.00, 92.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674752 reads in 322.784 CPU sec, 10.294 real sec
sorting block 52, #reads = 1080560 mem = 536878066 peak = 1231724544
[M::mem_process_seqs] Processed 674800 reads in 222.786 CPU sec, 7.148 real sec
[M::mem_process_seqs] Processed 674880 reads in 232.539 CPU sec, 7.476 real sec
sorting block 53, #reads = 1080377 mem = 536880798 peak = 1232912384
[M::process] read 674702 sequences (100000003 bp) in 3.252 CPU sec, 3.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285998, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (259.07, 133.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 211, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429)
[M::mem_pestat] mean and std.dev: (145.27, 93.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 538)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674624 sequences (100000165 bp) in 2.312 CPU sec, 2.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286093, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (259.75, 133.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674864 reads in 227.676 CPU sec, 7.230 real sec
[M::mem_process_seqs] Processed 674702 reads in 128.453 CPU sec, 4.029 real sec
sorting block 54, #reads = 1080449 mem = 536879040 peak = 1232912384
[M::process] read 674560 sequences (100000098 bp) in 2.121 CPU sec, 2.135 real sec...
sorting block 55, #reads = 1080565 mem = 536878461 peak = 1232912384
[M::process] read 674588 sequences (100000272 bp) in 2.521 CPU sec, 2.611 real sec...
[M::process] read 674672 sequences (100000015 bp) in 1.520 CPU sec, 1.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285770, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.38, 137.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 16, 25)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 55)
[M::mem_pestat] mean and std.dev: (11.57, 6.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 70)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674588 reads in 116.832 CPU sec, 3.733 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284675, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.79, 135.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674488 sequences (100000079 bp) in 2.304 CPU sec, 2.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285729, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.84, 137.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674624 reads in 346.722 CPU sec, 11.027 real sec
[M::mem_process_seqs] Processed 674560 reads in 243.244 CPU sec, 7.739 real sec
sorting block 56, #reads = 1079890 mem = 536882143 peak = 1232912384
sorting block 57, #reads = 1080333 mem = 536877468 peak = 1250529280
[M::process] read 674608 sequences (100000226 bp) in 2.171 CPU sec, 2.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285880, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.63, 138.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674990 sequences (100000075 bp) in 1.580 CPU sec, 1.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285127, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.01, 138.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286119, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.82, 139.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674672 reads in 319.462 CPU sec, 10.222 real sec
[M::mem_process_seqs] Processed 674488 reads in 220.349 CPU sec, 7.076 real sec
[M::mem_process_seqs] Processed 674608 reads in 229.873 CPU sec, 7.404 real sec
sorting block 58, #reads = 1080185 mem = 536879214 peak = 1250529280
[M::process] read 675872 sequences (100000194 bp) in 2.978 CPU sec, 3.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286572, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.40, 130.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 26, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (123.79, 142.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675946 sequences (100000129 bp) in 2.010 CPU sec, 2.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286687, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (240.57, 116.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674990 reads in 215.075 CPU sec, 6.809 real sec
sorting block 59, #reads = 1080075 mem = 536880312 peak = 1257689088
[M::mem_process_seqs] Processed 675872 reads in 121.967 CPU sec, 3.814 real sec
[M::process] read 675730 sequences (100000237 bp) in 2.030 CPU sec, 2.040 real sec...
sorting block 60, #reads = 1080641 mem = 536881375 peak = 1258049536
[M::process] read 675664 sequences (100000153 bp) in 2.504 CPU sec, 2.600 real sec...
[M::process] read 675756 sequences (100000191 bp) in 1.999 CPU sec, 2.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286624, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.02, 118.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675664 reads in 114.648 CPU sec, 3.647 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286656, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (241.86, 117.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675708 sequences (100000109 bp) in 2.101 CPU sec, 2.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287005, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.57, 117.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675946 reads in 325.492 CPU sec, 10.326 real sec
sorting block 61, #reads = 1081079 mem = 536884306 peak = 1258049536
[M::mem_process_seqs] Processed 675730 reads in 230.628 CPU sec, 7.318 real sec
sorting block 62, #reads = 1081142 mem = 536882257 peak = 1258049536
[M::process] read 675532 sequences (100000117 bp) in 2.279 CPU sec, 2.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286521, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.86, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 182, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596)
[M::mem_pestat] mean and std.dev: (96.62, 94.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675342 sequences (100000047 bp) in 1.600 CPU sec, 1.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286561, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.49, 119.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 101, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (203.91, 233.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1311)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286684, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.07, 123.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (72, 431, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1878)
[M::mem_pestat] mean and std.dev: (374.67, 258.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2480)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675756 reads in 319.034 CPU sec, 10.220 real sec
[M::mem_process_seqs] Processed 675708 reads in 221.921 CPU sec, 7.132 real sec
[M::mem_process_seqs] Processed 675532 reads in 231.567 CPU sec, 7.457 real sec
sorting block 63, #reads = 1080525 mem = 536878809 peak = 1258049536
[M::process] read 675462 sequences (100000089 bp) in 2.944 CPU sec, 3.263 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286228, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.27, 122.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675566 sequences (100000099 bp) in 2.222 CPU sec, 2.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286530, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.64, 121.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 388, 1750)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5234)
[M::mem_pestat] mean and std.dev: (897.75, 1400.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6976)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675420 sequences (100000114 bp) in 1.720 CPU sec, 1.719 real sec...
[M::mem_process_seqs] Processed 675342 reads in 230.159 CPU sec, 7.291 real sec
sorting block 64, #reads = 1081415 mem = 536881057 peak = 1258049536
[M::mem_process_seqs] Processed 675462 reads in 128.208 CPU sec, 4.027 real sec
sorting block 65, #reads = 1080712 mem = 536878638 peak = 1258049536
[M::process] read 675534 sequences (100000105 bp) in 2.693 CPU sec, 2.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286720, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.76, 121.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 586, 1104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2966)
[M::mem_pestat] mean and std.dev: (417.55, 387.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3897)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675322 sequences (100000288 bp) in 2.020 CPU sec, 2.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286321, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.44, 122.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286499, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.05, 121.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675566 reads in 295.768 CPU sec, 9.432 real sec
[M::mem_process_seqs] Processed 675420 reads in 200.830 CPU sec, 6.431 real sec
[M::mem_process_seqs] Processed 675534 reads in 209.605 CPU sec, 6.735 real sec
sorting block 66, #reads = 1081122 mem = 536880246 peak = 1258049536
sorting block 67, #reads = 1081071 mem = 536878935 peak = 1281478656
[M::process] read 675316 sequences (100000156 bp) in 2.863 CPU sec, 2.964 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286399, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.88, 123.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 23, 121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319)
[M::mem_pestat] mean and std.dev: (40.89, 44.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 418)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675370 sequences (100000205 bp) in 2.130 CPU sec, 2.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285960, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.91, 123.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675418 sequences (100000292 bp) in 2.010 CPU sec, 2.013 real sec...
[M::mem_process_seqs] Processed 675322 reads in 228.453 CPU sec, 7.213 real sec
[M::mem_process_seqs] Processed 675316 reads in 126.797 CPU sec, 3.988 real sec
sorting block 68, #reads = 1080693 mem = 536878585 peak = 1281478656
[M::process] read 675194 sequences (100000270 bp) in 2.522 CPU sec, 2.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285877, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.41, 122.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 199, 599)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1763)
[M::mem_pestat] mean and std.dev: (354.27, 358.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2345)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675266 sequences (100000007 bp) in 1.620 CPU sec, 1.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286077, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.47, 122.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 205, 626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1794)
[M::mem_pestat] mean and std.dev: (345.23, 384.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2378)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286143, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.62, 125.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 42, 626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1862)
[M::mem_pestat] mean and std.dev: (93.44, 189.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2480)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675370 reads in 323.347 CPU sec, 10.342 real sec
[M::mem_process_seqs] Processed 675418 reads in 222.075 CPU sec, 7.133 real sec
[M::mem_process_seqs] Processed 675194 reads in 231.526 CPU sec, 7.456 real sec
sorting block 69, #reads = 1080750 mem = 536880833 peak = 1281478656
sorting block 70, #reads = 1081150 mem = 536878089 peak = 1281478656
[M::process] read 675222 sequences (100000070 bp) in 2.956 CPU sec, 3.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285750, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.05, 125.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 61, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236)
[M::mem_pestat] mean and std.dev: (63.90, 55.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 302)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675162 sequences (100000124 bp) in 1.920 CPU sec, 1.921 real sec...
[M::process] read 675228 sequences (100000261 bp) in 1.490 CPU sec, 1.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285628, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.47, 125.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 51, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 119)
[M::mem_pestat] mean and std.dev: (39.00, 18.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 148)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675266 reads in 215.870 CPU sec, 6.841 real sec
[M::mem_process_seqs] Processed 675222 reads in 114.858 CPU sec, 3.630 real sec
sorting block 71, #reads = 1080672 mem = 536879422 peak = 1281478656
[M::process] read 675120 sequences (100000234 bp) in 1.964 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285510, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.96, 127.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 50, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 232)
[M::mem_pestat] mean and std.dev: (49.25, 38.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 303)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675016 sequences (100000113 bp) in 1.530 CPU sec, 1.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285762, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.39, 127.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285926, 23, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.02, 128.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 9, 50)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 144)
[M::mem_pestat] mean and std.dev: (11.94, 13.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 191)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675162 reads in 282.909 CPU sec, 9.059 real sec
[M::mem_process_seqs] Processed 675228 reads in 196.294 CPU sec, 6.305 real sec
[M::mem_process_seqs] Processed 675120 reads in 205.265 CPU sec, 6.614 real sec
sorting block 72, #reads = 1080500 mem = 536881032 peak = 1281478656
sorting block 73, #reads = 1080632 mem = 536879027 peak = 1281478656
[M::process] read 675184 sequences (100000198 bp) in 2.147 CPU sec, 2.272 real sec...
[M::process] read 675122 sequences (100000224 bp) in 2.013 CPU sec, 2.063 real sec...
[M::process] read 675100 sequences (100000089 bp) in 1.620 CPU sec, 1.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285698, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.51, 130.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (37, 101, 2978)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8860)
[M::mem_pestat] mean and std.dev: (798.25, 1259.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11801)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675184 reads in 108.896 CPU sec, 3.433 real sec
sorting block 74, #reads = 1080319 mem = 536878573 peak = 1281478656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285491, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.45, 129.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 94, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (186.50, 228.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1338)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675170 sequences (100000162 bp) in 1.581 CPU sec, 1.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285910, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.14, 130.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (88, 276, 882)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2470)
[M::mem_pestat] mean and std.dev: (373.15, 381.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3264)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675016 reads in 303.570 CPU sec, 9.642 real sec
[M::mem_process_seqs] Processed 675122 reads in 122.096 CPU sec, 3.918 real sec
sorting block 75, #reads = 1080733 mem = 536876362 peak = 1281478656
[M::process] read 674896 sequences (100000008 bp) in 2.156 CPU sec, 2.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285760, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.28, 131.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 61, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 121)
[M::mem_pestat] mean and std.dev: (45.18, 22.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 151)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674980 sequences (100000135 bp) in 1.470 CPU sec, 1.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285705, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.33, 131.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285650, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.83, 131.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675100 reads in 282.740 CPU sec, 9.050 real sec
[M::mem_process_seqs] Processed 675170 reads in 195.935 CPU sec, 6.296 real sec
[M::mem_process_seqs] Processed 674896 reads in 205.146 CPU sec, 6.618 real sec
sorting block 76, #reads = 1080440 mem = 536879052 peak = 1281478656
[M::process] read 674968 sequences (100000136 bp) in 2.442 CPU sec, 2.728 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285518, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.45, 130.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 77, #reads = 1080865 mem = 536879367 peak = 1281478656
[M::process] read 674920 sequences (100000173 bp) in 1.838 CPU sec, 1.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285508, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.45, 130.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674852 sequences (100000275 bp) in 1.460 CPU sec, 1.460 real sec...
[M::mem_process_seqs] Processed 674980 reads in 196.852 CPU sec, 6.261 real sec
[M::mem_process_seqs] Processed 674968 reads in 110.713 CPU sec, 3.504 real sec
sorting block 78, #reads = 1080676 mem = 536877783 peak = 1281478656
[M::process] read 675000 sequences (100000107 bp) in 2.133 CPU sec, 2.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286091, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.17, 131.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 14, 20)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 54)
[M::mem_pestat] mean and std.dev: (13.00, 9.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 71)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674904 sequences (100000048 bp) in 1.460 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285790, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (258.95, 132.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 88, 168)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (82.38, 60.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 597)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285870, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.08, 131.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 420, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (250.00, 173.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674920 reads in 278.470 CPU sec, 8.911 real sec
[M::mem_process_seqs] Processed 674852 reads in 195.092 CPU sec, 6.272 real sec
[M::mem_process_seqs] Processed 675000 reads in 204.247 CPU sec, 6.588 real sec
sorting block 79, #reads = 1080505 mem = 536881116 peak = 1281478656
sorting block 80, #reads = 1080180 mem = 536878016 peak = 1281478656
[M::process] read 674734 sequences (100000253 bp) in 2.296 CPU sec, 2.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285657, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.07, 133.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674838 sequences (100000120 bp) in 1.670 CPU sec, 1.699 real sec...
[M::process] read 674564 sequences (100000043 bp) in 1.460 CPU sec, 1.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285726, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.88, 132.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674904 reads in 196.681 CPU sec, 6.258 real sec
[M::mem_process_seqs] Processed 674734 reads in 110.648 CPU sec, 3.511 real sec
sorting block 81, #reads = 1080755 mem = 536879658 peak = 1281478656
[M::process] read 674708 sequences (100000171 bp) in 1.982 CPU sec, 2.042 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285613, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.06, 132.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674808 sequences (100000156 bp) in 1.480 CPU sec, 1.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285941, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.41, 132.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286190, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.10, 133.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 37, 5722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17134)
[M::mem_pestat] mean and std.dev: (1445.92, 2350.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22840)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674838 reads in 274.527 CPU sec, 8.790 real sec
[M::mem_process_seqs] Processed 674708 reads in 191.129 CPU sec, 6.147 real sec
[M::mem_process_seqs] Processed 674564 reads in 200.209 CPU sec, 6.462 real sec
sorting block 82, #reads = 1080291 mem = 536880351 peak = 1281478656
sorting block 83, #reads = 1080742 mem = 536878644 peak = 1281478656
[M::process] read 674746 sequences (100000096 bp) in 2.720 CPU sec, 2.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286106, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.04, 134.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 145, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (195.69, 134.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674632 sequences (100000085 bp) in 1.739 CPU sec, 1.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285981, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.85, 132.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 556, 772)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2282)
[M::mem_pestat] mean and std.dev: (363.00, 357.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3037)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674524 sequences (100000128 bp) in 1.460 CPU sec, 1.465 real sec...
[M::mem_process_seqs] Processed 674808 reads in 192.617 CPU sec, 6.153 real sec
[M::mem_process_seqs] Processed 674746 reads in 107.925 CPU sec, 3.410 real sec
sorting block 84, #reads = 1080324 mem = 536878724 peak = 1281478656
[M::process] read 674562 sequences (100000118 bp) in 2.108 CPU sec, 2.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285535, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.60, 135.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (447, 447, 8627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24987)
[M::mem_pestat] mean and std.dev: (3304.36, 4027.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 33167)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674520 sequences (100000115 bp) in 1.420 CPU sec, 1.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285866, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.65, 136.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 78, 752)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2232)
[M::mem_pestat] mean and std.dev: (258.62, 345.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2972)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285592, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.58, 136.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 19, 32)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 58)
[M::mem_pestat] mean and std.dev: (15.44, 9.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 71)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674632 reads in 274.272 CPU sec, 8.778 real sec
[M::mem_process_seqs] Processed 674524 reads in 191.390 CPU sec, 6.160 real sec
sorting block 85, #reads = 1080827 mem = 536878580 peak = 1281478656
[M::mem_process_seqs] Processed 674562 reads in 200.380 CPU sec, 6.471 real sec
sorting block 86, #reads = 1080332 mem = 536878560 peak = 1281478656
[M::process] read 674482 sequences (100000278 bp) in 2.734 CPU sec, 2.866 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285127, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.24, 137.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674668 sequences (100000237 bp) in 1.890 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285512, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.05, 138.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674520 reads in 189.644 CPU sec, 6.043 real sec
[M::mem_process_seqs] Processed 674482 reads in 107.907 CPU sec, 3.403 real sec
[M::process] read 674518 sequences (100000192 bp) in 1.685 CPU sec, 1.706 real sec...
sorting block 87, #reads = 1080043 mem = 536878374 peak = 1281478656
[M::process] read 675480 sequences (100000040 bp) in 1.917 CPU sec, 1.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285508, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.55, 138.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 169, 598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1756)
[M::mem_pestat] mean and std.dev: (302.27, 272.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2335)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675640 sequences (100000262 bp) in 1.480 CPU sec, 1.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286114, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.69, 139.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286915, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (245.42, 120.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674668 reads in 265.528 CPU sec, 8.510 real sec
sorting block 88, #reads = 1079657 mem = 536881182 peak = 1281478656
[M::mem_process_seqs] Processed 674518 reads in 183.817 CPU sec, 5.911 real sec
[M::mem_process_seqs] Processed 675480 reads in 192.633 CPU sec, 6.216 real sec
sorting block 89, #reads = 1079385 mem = 536878776 peak = 1281478656
[M::process] read 675774 sequences (100000213 bp) in 2.358 CPU sec, 2.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286653, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (239.37, 113.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (37, 421, 645)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1861)
[M::mem_pestat] mean and std.dev: (478.55, 486.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2469)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675614 sequences (100000117 bp) in 1.703 CPU sec, 1.734 real sec...
[M::process] read 675686 sequences (100000253 bp) in 1.490 CPU sec, 1.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286760, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (241.04, 115.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 196, 801)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2337)
[M::mem_pestat] mean and std.dev: (525.67, 508.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3105)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675640 reads in 187.030 CPU sec, 5.953 real sec
sorting block 90, #reads = 1080897 mem = 536878381 peak = 1281478656
[M::mem_process_seqs] Processed 675774 reads in 104.779 CPU sec, 3.320 real sec
sorting block 91, #reads = 1081309 mem = 536880310 peak = 1281478656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286780, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.58, 116.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675668 sequences (100000140 bp) in 2.623 CPU sec, 2.716 real sec...
[M::process] read 675644 sequences (100000185 bp) in 1.490 CPU sec, 1.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286717, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.54, 117.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675614 reads in 187.169 CPU sec, 5.960 real sec
[M::mem_process_seqs] Processed 675686 reads in 105.733 CPU sec, 3.373 real sec
sorting block 92, #reads = 1081141 mem = 536883956 peak = 1281478656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287024, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (243.15, 117.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 309, 1383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4105)
[M::mem_pestat] mean and std.dev: (504.21, 567.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5466)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675276 sequences (100000167 bp) in 2.116 CPU sec, 2.197 real sec...
[M::process] read 675496 sequences (100000001 bp) in 1.540 CPU sec, 1.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286764, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.53, 118.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 104, 776)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2302)
[M::mem_pestat] mean and std.dev: (363.46, 400.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3065)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675668 reads in 187.033 CPU sec, 5.976 real sec
[M::mem_process_seqs] Processed 675644 reads in 105.805 CPU sec, 3.378 real sec
sorting block 93, #reads = 1080992 mem = 536879988 peak = 1281478656
[M::process] read 675520 sequences (100000047 bp) in 2.288 CPU sec, 2.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285729, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.23, 120.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 182, 1528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4520)
[M::mem_pestat] mean and std.dev: (507.73, 633.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6016)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675462 sequences (100000017 bp) in 1.510 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286156, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (244.59, 119.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286451, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.48, 119.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 11, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158)
[M::mem_pestat] mean and std.dev: (154.58, 275.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1541)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675276 reads in 278.256 CPU sec, 8.913 real sec
[M::mem_process_seqs] Processed 675496 reads in 194.015 CPU sec, 6.232 real sec
[M::mem_process_seqs] Processed 675520 reads in 202.943 CPU sec, 6.545 real sec
sorting block 94, #reads = 1080767 mem = 536880637 peak = 1281478656
sorting block 95, #reads = 1080942 mem = 536879586 peak = 1281478656
[M::process] read 675320 sequences (100000045 bp) in 2.668 CPU sec, 2.843 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286389, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (245.45, 119.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675340 sequences (100000228 bp) in 2.110 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286254, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.47, 120.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 61, 2670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7982)
[M::mem_pestat] mean and std.dev: (751.45, 1175.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10638)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675462 reads in 194.170 CPU sec, 6.194 real sec
[M::mem_process_seqs] Processed 675320 reads in 110.652 CPU sec, 3.497 real sec
[M::process] read 675400 sequences (100000184 bp) in 1.642 CPU sec, 1.659 real sec...
sorting block 96, #reads = 1080773 mem = 536881609 peak = 1281478656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286085, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.44, 121.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675316 sequences (100000010 bp) in 2.792 CPU sec, 2.909 real sec...
[M::process] read 675380 sequences (100000119 bp) in 1.490 CPU sec, 1.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286216, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.68, 121.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675340 reads in 193.896 CPU sec, 6.176 real sec
[M::mem_process_seqs] Processed 675400 reads in 107.143 CPU sec, 3.420 real sec
sorting block 97, #reads = 1080950 mem = 536878396 peak = 1281478656
sorting block 98, #reads = 1081347 mem = 536878120 peak = 1281478656
[M::process] read 675164 sequences (100000122 bp) in 2.084 CPU sec, 2.126 real sec...
[M::process] read 675194 sequences (100000172 bp) in 1.530 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286136, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.26, 122.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675164 reads in 102.239 CPU sec, 3.264 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286145, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.82, 121.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675088 sequences (100000110 bp) in 1.653 CPU sec, 1.663 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286545, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.43, 122.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675316 reads in 287.443 CPU sec, 9.161 real sec
[M::mem_process_seqs] Processed 675380 reads in 203.807 CPU sec, 6.510 real sec
sorting block 99, #reads = 1081127 mem = 536878583 peak = 1281478656
[M::process] read 675056 sequences (100000257 bp) in 2.064 CPU sec, 2.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285859, 22, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.98, 123.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 31, 1690)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5054)
[M::mem_pestat] mean and std.dev: (490.14, 736.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6736)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675074 sequences (100000275 bp) in 1.510 CPU sec, 1.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286067, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.89, 123.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285903, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.78, 124.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675194 reads in 280.301 CPU sec, 8.977 real sec
sorting block 100, #reads = 1080779 mem = 536877867 peak = 1281478656
[M::mem_process_seqs] Processed 675088 reads in 196.638 CPU sec, 6.326 real sec
[M::mem_process_seqs] Processed 675056 reads in 205.688 CPU sec, 6.642 real sec
sorting block 101, #reads = 1080715 mem = 536878277 peak = 1281478656
[M::process] read 674938 sequences (100000252 bp) in 2.356 CPU sec, 2.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285750, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (252.16, 125.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674968 sequences (100000205 bp) in 1.731 CPU sec, 1.746 real sec...
[M::process] read 674930 sequences (100000187 bp) in 1.440 CPU sec, 1.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285904, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.84, 126.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675074 reads in 194.545 CPU sec, 6.196 real sec
[M::mem_process_seqs] Processed 674938 reads in 109.136 CPU sec, 3.459 real sec
sorting block 102, #reads = 1080642 mem = 536879042 peak = 1281478656
sorting block 103, #reads = 1079889 mem = 536878686 peak = 1281478656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285675, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.86, 127.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (336, 367, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (380.60, 210.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674866 sequences (100000028 bp) in 2.774 CPU sec, 2.902 real sec...
[M::process] read 675002 sequences (100000114 bp) in 1.560 CPU sec, 1.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285959, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (254.29, 127.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674968 reads in 194.982 CPU sec, 6.209 real sec
[M::mem_process_seqs] Processed 674930 reads in 108.727 CPU sec, 3.473 real sec
sorting block 104, #reads = 1080831 mem = 536878081 peak = 1281478656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285665, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.63, 128.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675012 sequences (100000118 bp) in 2.201 CPU sec, 2.347 real sec...
[M::process] read 674954 sequences (100000298 bp) in 1.558 CPU sec, 1.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285537, 16, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.27, 127.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 6884, 6884)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20520)
[M::mem_pestat] mean and std.dev: (3720.25, 3227.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 27338)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674866 reads in 193.591 CPU sec, 6.181 real sec
[M::mem_process_seqs] Processed 675002 reads in 109.263 CPU sec, 3.484 real sec
sorting block 105, #reads = 1080426 mem = 536877849 peak = 1281478656
[M::process] read 674920 sequences (100000240 bp) in 2.176 CPU sec, 2.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285590, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.89, 128.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (370, 574, 611)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (457.69, 189.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1334)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674892 sequences (100000199 bp) in 1.540 CPU sec, 1.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285505, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.78, 129.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 232, 2480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7396)
[M::mem_pestat] mean and std.dev: (654.62, 1003.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9854)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285160, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.72, 128.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675012 reads in 282.009 CPU sec, 9.032 real sec
[M::mem_process_seqs] Processed 674954 reads in 198.064 CPU sec, 6.366 real sec
[M::mem_process_seqs] Processed 674920 reads in 207.146 CPU sec, 6.682 real sec
sorting block 106, #reads = 1080583 mem = 536876292 peak = 1281478656
sorting block 107, #reads = 1080557 mem = 536877764 peak = 1281478656
[M::process] read 674870 sequences (100000237 bp) in 2.134 CPU sec, 2.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285708, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.53, 130.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674926 sequences (100000166 bp) in 1.750 CPU sec, 1.782 real sec...
[M::process] read 674908 sequences (100000189 bp) in 1.470 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285875, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.17, 129.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 8, 14)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 34)
[M::mem_pestat] mean and std.dev: (7.75, 4.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 44)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674892 reads in 196.191 CPU sec, 6.240 real sec
[M::mem_process_seqs] Processed 674870 reads in 110.997 CPU sec, 3.522 real sec
sorting block 108, #reads = 1080256 mem = 536876610 peak = 1281478656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285289, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.07, 130.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 18, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499)
[M::mem_pestat] mean and std.dev: (242.18, 267.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1997)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674812 sequences (100000203 bp) in 2.840 CPU sec, 2.968 real sec...
[M::process] read 674832 sequences (100000163 bp) in 1.560 CPU sec, 1.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285846, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.77, 130.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 218, 1438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4250)
[M::mem_pestat] mean and std.dev: (512.23, 663.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5656)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674926 reads in 194.110 CPU sec, 6.183 real sec
[M::mem_process_seqs] Processed 674908 reads in 108.526 CPU sec, 3.459 real sec
sorting block 109, #reads = 1080077 mem = 536880186 peak = 1281478656
sorting block 110, #reads = 1080032 mem = 536878721 peak = 1281478656
[M::process] read 674762 sequences (100000224 bp) in 2.254 CPU sec, 2.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285801, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.96, 130.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674690 sequences (100000053 bp) in 1.550 CPU sec, 1.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285068, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.35, 131.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674812 reads in 195.173 CPU sec, 6.231 real sec
[M::mem_process_seqs] Processed 674832 reads in 109.869 CPU sec, 3.509 real sec
sorting block 111, #reads = 1080316 mem = 536881954 peak = 1281478656
[M::process] read 674714 sequences (100000238 bp) in 2.016 CPU sec, 2.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285640, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.05, 133.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 25, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272)
[M::mem_pestat] mean and std.dev: (121.33, 191.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1695)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674596 sequences (100000212 bp) in 1.550 CPU sec, 1.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285638, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.97, 132.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 187, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1567)
[M::mem_pestat] mean and std.dev: (309.00, 298.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2082)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285507, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.69, 134.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674762 reads in 285.541 CPU sec, 9.152 real sec
[M::mem_process_seqs] Processed 674714 reads in 199.545 CPU sec, 6.411 real sec
[M::mem_process_seqs] Processed 674690 reads in 208.794 CPU sec, 6.731 real sec
sorting block 112, #reads = 1080339 mem = 536878839 peak = 1281478656
[M::process] read 674534 sequences (100000260 bp) in 2.319 CPU sec, 2.516 real sec...
sorting block 113, #reads = 1080544 mem = 536878740 peak = 1281478656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285922, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.65, 133.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674648 sequences (100000083 bp) in 1.726 CPU sec, 1.733 real sec...
[M::process] read 674574 sequences (100000024 bp) in 1.500 CPU sec, 1.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285914, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.94, 135.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 82, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2118)
[M::mem_pestat] mean and std.dev: (311.57, 424.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2811)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674596 reads in 196.499 CPU sec, 6.255 real sec
[M::mem_process_seqs] Processed 674534 reads in 109.513 CPU sec, 3.462 real sec
sorting block 114, #reads = 1080948 mem = 536880459 peak = 1281478656
[M::process] read 674608 sequences (100000194 bp) in 2.710 CPU sec, 2.831 real sec...
[M::process] read 674520 sequences (100000186 bp) in 1.510 CPU sec, 1.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285230, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.25, 135.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674574 reads in 103.963 CPU sec, 3.302 real sec
sorting block 115, #reads = 1079886 mem = 536881148 peak = 1281478656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285048, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.17, 134.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674416 sequences (100000135 bp) in 1.564 CPU sec, 1.568 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285701, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.27, 135.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674648 reads in 294.429 CPU sec, 9.384 real sec
[M::mem_process_seqs] Processed 674608 reads in 118.379 CPU sec, 3.799 real sec
sorting block 116, #reads = 1080117 mem = 536877545 peak = 1281478656
[M::process] read 674546 sequences (100000085 bp) in 2.316 CPU sec, 2.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285531, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.62, 134.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 42, 192)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (85.12, 103.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 693)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674556 sequences (100000163 bp) in 1.510 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285296, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.13, 135.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674520 reads in 192.258 CPU sec, 6.131 real sec
[M::mem_process_seqs] Processed 674416 reads in 108.400 CPU sec, 3.470 real sec
sorting block 117, #reads = 1080473 mem = 536878970 peak = 1281478656
[M::process] read 674540 sequences (100000271 bp) in 2.100 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284897, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.97, 135.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674938 sequences (100000255 bp) in 1.540 CPU sec, 1.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285813, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.62, 139.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 46, 1537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4583)
[M::mem_pestat] mean and std.dev: (430.42, 642.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6106)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285715, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.15, 138.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 128, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (87.25, 62.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674546 reads in 273.684 CPU sec, 8.761 real sec
[M::mem_process_seqs] Processed 674556 reads in 190.820 CPU sec, 6.134 real sec
[M::mem_process_seqs] Processed 674540 reads in 200.010 CPU sec, 6.452 real sec
sorting block 118, #reads = 1079659 mem = 536878846 peak = 1281478656
sorting block 119, #reads = 1079940 mem = 536877964 peak = 1281478656
[M::process] read 676034 sequences (100000238 bp) in 2.381 CPU sec, 2.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286113, 19, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.68, 129.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 26, 27)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 71)
[M::mem_pestat] mean and std.dev: (14.93, 11.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 93)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675982 sequences (100000230 bp) in 1.814 CPU sec, 1.863 real sec...
[M::process] read 675996 sequences (100000163 bp) in 1.510 CPU sec, 1.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286377, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (237.61, 115.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 181, 816)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2110)
[M::mem_pestat] mean and std.dev: (348.73, 325.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2757)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674938 reads in 195.410 CPU sec, 6.208 real sec
[M::mem_process_seqs] Processed 676034 reads in 112.117 CPU sec, 3.549 real sec
sorting block 120, #reads = 1080028 mem = 536880042 peak = 1281478656
[M::process] read 675730 sequences (100000090 bp) in 2.476 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286257, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (238.13, 114.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675942 sequences (100000014 bp) in 1.520 CPU sec, 1.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286343, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (238.74, 115.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675982 reads in 193.116 CPU sec, 6.156 real sec
[M::mem_process_seqs] Processed 675996 reads in 108.084 CPU sec, 3.453 real sec
sorting block 121, #reads = 1080825 mem = 536879958 peak = 1281478656
[M::process] read 675918 sequences (100000244 bp) in 2.045 CPU sec, 2.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285533, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.25, 116.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675728 sequences (100000256 bp) in 1.520 CPU sec, 1.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285746, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (239.83, 117.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286490, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (238.94, 116.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 58, 128)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366)
[M::mem_pestat] mean and std.dev: (43.21, 44.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675730 reads in 285.902 CPU sec, 9.159 real sec
sorting block 122, #reads = 1081279 mem = 536879893 peak = 1281478656
[M::mem_process_seqs] Processed 675918 reads in 200.880 CPU sec, 6.456 real sec
[M::mem_process_seqs] Processed 675942 reads in 209.800 CPU sec, 6.763 real sec
sorting block 123, #reads = 1080739 mem = 536878344 peak = 1281478656
[M::process] read 675780 sequences (100000002 bp) in 2.416 CPU sec, 2.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285992, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.90, 119.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675664 sequences (100000138 bp) in 1.744 CPU sec, 1.758 real sec...
[M::process] read 675686 sequences (100000032 bp) in 1.470 CPU sec, 1.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285170, 13, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.35, 118.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 19)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 51)
[M::mem_pestat] mean and std.dev: (10.50, 11.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 67)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675728 reads in 201.743 CPU sec, 6.419 real sec
[M::mem_process_seqs] Processed 675780 reads in 113.425 CPU sec, 3.594 real sec
sorting block 124, #reads = 1081402 mem = 536884210 peak = 1281478656
sorting block 125, #reads = 1080682 mem = 536880383 peak = 1281478656
[M::process] read 675520 sequences (100000037 bp) in 1.953 CPU sec, 2.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285745, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.08, 120.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 4, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 175)
[M::mem_pestat] mean and std.dev: (22.89, 26.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 232)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675622 sequences (100000281 bp) in 1.540 CPU sec, 1.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285092, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.98, 120.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285551, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.89, 120.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675664 reads in 293.907 CPU sec, 9.415 real sec
[M::mem_process_seqs] Processed 675686 reads in 205.422 CPU sec, 6.609 real sec
[M::mem_process_seqs] Processed 675520 reads in 214.244 CPU sec, 6.915 real sec
sorting block 126, #reads = 1080427 mem = 536879446 peak = 1281478656
[M::process] read 675580 sequences (100000043 bp) in 2.375 CPU sec, 2.535 real sec...
sorting block 127, #reads = 1080812 mem = 536878879 peak = 1281478656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285175, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.98, 121.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675544 sequences (100000105 bp) in 1.788 CPU sec, 1.822 real sec...
[M::process] read 675602 sequences (100000281 bp) in 1.520 CPU sec, 1.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285250, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.35, 120.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675622 reads in 205.095 CPU sec, 6.530 real sec
[M::mem_process_seqs] Processed 675580 reads in 114.646 CPU sec, 3.632 real sec
sorting block 128, #reads = 1078966 mem = 536878715 peak = 1281478656
[M::process] read 675484 sequences (100000126 bp) in 2.462 CPU sec, 2.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286061, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.04, 121.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 74, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (99.19, 133.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675426 sequences (100000211 bp) in 1.510 CPU sec, 1.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285722, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.47, 120.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675544 reads in 200.362 CPU sec, 6.392 real sec
[M::mem_process_seqs] Processed 675602 reads in 112.804 CPU sec, 3.610 real sec
sorting block 129, #reads = 1080834 mem = 536880466 peak = 1281478656
[M::process] read 675420 sequences (100000259 bp) in 2.069 CPU sec, 2.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285664, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.59, 121.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675314 sequences (100000103 bp) in 1.550 CPU sec, 1.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285165, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (245.57, 122.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285132, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.27, 124.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675484 reads in 297.286 CPU sec, 9.525 real sec
[M::mem_process_seqs] Processed 675426 reads in 208.249 CPU sec, 6.698 real sec
[M::mem_process_seqs] Processed 675420 reads in 216.963 CPU sec, 7.004 real sec
sorting block 130, #reads = 1080921 mem = 536881688 peak = 1281478656
sorting block 131, #reads = 1080500 mem = 536880187 peak = 1281478656
[M::process] read 675416 sequences (100000209 bp) in 2.289 CPU sec, 2.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284681, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.73, 124.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675198 sequences (100000115 bp) in 1.794 CPU sec, 1.833 real sec...
[M::process] read 675232 sequences (100000017 bp) in 1.480 CPU sec, 1.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285272, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.41, 127.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 88, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (112.14, 122.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 741)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675314 reads in 210.925 CPU sec, 6.714 real sec
[M::mem_process_seqs] Processed 675416 reads in 115.584 CPU sec, 3.670 real sec
sorting block 132, #reads = 1080415 mem = 536879213 peak = 1281478656
[M::process] read 675308 sequences (100000250 bp) in 2.008 CPU sec, 2.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284900, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.03, 126.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675192 sequences (100000051 bp) in 1.520 CPU sec, 1.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285190, 10, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.40, 127.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 33, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1241)
[M::mem_pestat] mean and std.dev: (112.62, 167.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1651)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285017, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.65, 127.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675198 reads in 299.265 CPU sec, 9.593 real sec
[M::mem_process_seqs] Processed 675232 reads in 207.994 CPU sec, 6.689 real sec
sorting block 133, #reads = 1080720 mem = 536877097 peak = 1281478656
[M::mem_process_seqs] Processed 675308 reads in 216.982 CPU sec, 7.001 real sec
sorting block 134, #reads = 1080266 mem = 536877974 peak = 1294827520
[M::process] read 675306 sequences (100000237 bp) in 2.434 CPU sec, 2.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285044, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.03, 127.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 16, 1318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3936)
[M::mem_pestat] mean and std.dev: (435.08, 602.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5245)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675270 sequences (100000291 bp) in 1.895 CPU sec, 2.033 real sec...
[M::process] read 675204 sequences (100000034 bp) in 1.490 CPU sec, 1.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285296, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.78, 127.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (626, 658, 5233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14447)
[M::mem_pestat] mean and std.dev: (1782.45, 2126.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19054)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675192 reads in 212.398 CPU sec, 6.746 real sec
[M::mem_process_seqs] Processed 675306 reads in 116.907 CPU sec, 3.701 real sec
sorting block 135, #reads = 1081018 mem = 536878583 peak = 1294827520
[M::process] read 675160 sequences (100000071 bp) in 1.913 CPU sec, 1.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285278, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.68, 128.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 197, 626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1860)
[M::mem_pestat] mean and std.dev: (346.70, 430.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2477)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675024 sequences (100000159 bp) in 1.500 CPU sec, 1.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285135, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.14, 128.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675270 reads in 205.673 CPU sec, 6.568 real sec
[M::mem_process_seqs] Processed 675204 reads in 114.284 CPU sec, 3.680 real sec
sorting block 136, #reads = 1080959 mem = 536877545 peak = 1294827520
sorting block 137, #reads = 1080328 mem = 536878974 peak = 1294827520
[M::process] read 674888 sequences (100000185 bp) in 1.889 CPU sec, 1.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285008, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.51, 127.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675028 sequences (100000028 bp) in 1.570 CPU sec, 1.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285095, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.01, 129.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284548, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.44, 133.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 175, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194)
[M::mem_pestat] mean and std.dev: (198.80, 212.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1581)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675160 reads in 301.957 CPU sec, 9.682 real sec
[M::mem_process_seqs] Processed 675024 reads in 211.706 CPU sec, 6.809 real sec
[M::mem_process_seqs] Processed 674888 reads in 220.827 CPU sec, 7.129 real sec
sorting block 138, #reads = 1080463 mem = 536878938 peak = 1294827520
[M::process] read 675076 sequences (100000042 bp) in 2.143 CPU sec, 2.255 real sec...
sorting block 139, #reads = 1080503 mem = 536877799 peak = 1294827520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284473, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.64, 129.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675092 sequences (100000131 bp) in 1.662 CPU sec, 1.755 real sec...
[M::process] read 675158 sequences (100000178 bp) in 1.490 CPU sec, 1.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285186, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.22, 130.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675028 reads in 209.673 CPU sec, 6.678 real sec
[M::mem_process_seqs] Processed 675076 reads in 114.751 CPU sec, 3.663 real sec
sorting block 140, #reads = 1080241 mem = 536879481 peak = 1294827520
[M::process] read 674984 sequences (100000039 bp) in 2.540 CPU sec, 2.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284681, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.56, 130.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674908 sequences (100000199 bp) in 1.500 CPU sec, 1.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284610, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.20, 130.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 95, 5473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16395)
[M::mem_pestat] mean and std.dev: (1415.75, 2343.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21856)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675092 reads in 206.585 CPU sec, 6.592 real sec
[M::mem_process_seqs] Processed 675158 reads in 114.593 CPU sec, 3.663 real sec
sorting block 141, #reads = 1080204 mem = 536880506 peak = 1294827520
[M::process] read 675094 sequences (100000216 bp) in 2.295 CPU sec, 2.418 real sec...
[M::process] read 675012 sequences (100000043 bp) in 1.569 CPU sec, 1.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284709, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.23, 132.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 184, 184)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (135.92, 112.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 712)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675094 reads in 107.811 CPU sec, 3.433 real sec
sorting block 142, #reads = 1080336 mem = 536878701 peak = 1294827520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285239, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.34, 132.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 43, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 131)
[M::mem_pestat] mean and std.dev: (39.25, 23.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 166)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675060 sequences (100000109 bp) in 1.609 CPU sec, 1.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285089, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.01, 132.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674984 reads in 307.491 CPU sec, 9.808 real sec
[M::mem_process_seqs] Processed 674908 reads in 217.852 CPU sec, 6.952 real sec
sorting block 143, #reads = 1080766 mem = 536878303 peak = 1294827520
[M::process] read 674812 sequences (100000239 bp) in 1.863 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285112, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.42, 131.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674964 sequences (100000223 bp) in 1.500 CPU sec, 1.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284694, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.66, 135.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 485, 1121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3139)
[M::mem_pestat] mean and std.dev: (616.69, 679.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4148)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284777, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.27, 132.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675012 reads in 317.583 CPU sec, 10.181 real sec
[M::mem_process_seqs] Processed 674812 reads in 231.517 CPU sec, 7.450 real sec
[M::mem_process_seqs] Processed 675060 reads in 241.549 CPU sec, 7.794 real sec
sorting block 144, #reads = 1080116 mem = 536880898 peak = 1294827520
[M::process] read 674882 sequences (100000127 bp) in 2.459 CPU sec, 2.591 real sec...
sorting block 145, #reads = 1080014 mem = 536880813 peak = 1297051648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285522, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (259.12, 134.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674948 sequences (100000226 bp) in 2.291 CPU sec, 2.447 real sec...
[M::process] read 674838 sequences (100000032 bp) in 1.750 CPU sec, 1.757 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284642, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (260.51, 136.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674964 reads in 247.652 CPU sec, 7.892 real sec
[M::mem_process_seqs] Processed 674882 reads in 135.912 CPU sec, 4.314 real sec
sorting block 146, #reads = 1080361 mem = 536878488 peak = 1297051648
[M::process] read 674848 sequences (100000002 bp) in 2.837 CPU sec, 2.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285500, 22, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (260.29, 136.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (357, 533, 591)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059)
[M::mem_pestat] mean and std.dev: (379.18, 171.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674924 sequences (100000005 bp) in 2.120 CPU sec, 2.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284917, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (261.25, 138.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285187, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (261.43, 137.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674948 reads in 345.100 CPU sec, 11.018 real sec
[M::mem_process_seqs] Processed 674848 reads in 240.722 CPU sec, 7.714 real sec
[M::mem_process_seqs] Processed 674838 reads in 250.787 CPU sec, 8.062 real sec
sorting block 147, #reads = 1080144 mem = 536879361 peak = 1297051648
sorting block 148, #reads = 1080013 mem = 536880706 peak = 1297051648
[M::process] read 676214 sequences (100000119 bp) in 3.194 CPU sec, 3.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285234, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (258.80, 137.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676142 sequences (100000026 bp) in 1.745 CPU sec, 1.912 real sec...
[M::process] read 676122 sequences (100000272 bp) in 1.580 CPU sec, 1.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286510, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (234.84, 112.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (72, 348, 795)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2241)
[M::mem_pestat] mean and std.dev: (354.50, 312.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2964)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674924 reads in 240.219 CPU sec, 7.641 real sec
[M::mem_process_seqs] Processed 676214 reads in 129.253 CPU sec, 4.090 real sec
sorting block 149, #reads = 1079981 mem = 536878992 peak = 1297051648
[M::process] read 676054 sequences (100000290 bp) in 2.781 CPU sec, 2.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286697, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (236.32, 114.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 81, 176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (75.50, 61.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 638)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675964 sequences (100000098 bp) in 1.970 CPU sec, 1.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286204, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (237.66, 115.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286530, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (236.88, 115.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676142 reads in 327.668 CPU sec, 10.466 real sec
[M::mem_process_seqs] Processed 676054 reads in 229.176 CPU sec, 7.340 real sec
sorting block 150, #reads = 1081307 mem = 536878633 peak = 1297051648
[M::mem_process_seqs] Processed 676122 reads in 238.371 CPU sec, 7.651 real sec
sorting block 151, #reads = 1081103 mem = 536880742 peak = 1300062208
[M::process] read 675984 sequences (100000280 bp) in 3.026 CPU sec, 3.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286435, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (239.64, 117.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675912 sequences (100000065 bp) in 2.110 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286481, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.48, 116.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675964 reads in 230.199 CPU sec, 7.287 real sec
[M::process] read 675770 sequences (100000086 bp) in 2.040 CPU sec, 2.043 real sec...
[M::mem_process_seqs] Processed 675984 reads in 126.672 CPU sec, 3.971 real sec
sorting block 152, #reads = 1080873 mem = 536877890 peak = 1301729280
[M::process] read 675796 sequences (100000295 bp) in 2.611 CPU sec, 2.673 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286128, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (240.66, 118.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675698 sequences (100000186 bp) in 2.070 CPU sec, 2.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286208, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.45, 120.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 879, 879)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2611)
[M::mem_pestat] mean and std.dev: (530.41, 428.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3477)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286112, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.28, 119.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 21, 46)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 120)
[M::mem_pestat] mean and std.dev: (25.11, 21.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 157)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675912 reads in 327.512 CPU sec, 10.454 real sec
sorting block 153, #reads = 1080910 mem = 536879311 peak = 1301729280
[M::mem_process_seqs] Processed 675796 reads in 226.539 CPU sec, 7.256 real sec
[M::mem_process_seqs] Processed 675770 reads in 235.954 CPU sec, 7.574 real sec
sorting block 154, #reads = 1080576 mem = 536878891 peak = 1301729280
[M::process] read 675666 sequences (100000079 bp) in 2.445 CPU sec, 2.638 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286421, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.05, 119.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675636 sequences (100000121 bp) in 2.104 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286076, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.77, 119.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675682 sequences (100000094 bp) in 2.050 CPU sec, 2.049 real sec...
[M::mem_process_seqs] Processed 675698 reads in 220.623 CPU sec, 6.992 real sec
sorting block 155, #reads = 1081339 mem = 536881632 peak = 1301729280
[M::mem_process_seqs] Processed 675666 reads in 120.595 CPU sec, 3.794 real sec
sorting block 156, #reads = 1079745 mem = 536877853 peak = 1301729280
[M::process] read 675522 sequences (100000140 bp) in 2.120 CPU sec, 2.243 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285520, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.91, 121.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (293, 2077, 8738)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25628)
[M::mem_pestat] mean and std.dev: (3165.82, 3518.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 34073)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675568 sequences (100000006 bp) in 1.560 CPU sec, 1.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285770, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.39, 120.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 104, 1401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4151)
[M::mem_pestat] mean and std.dev: (476.64, 579.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5526)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675636 reads in 231.940 CPU sec, 7.400 real sec
[M::mem_process_seqs] Processed 675682 reads in 127.748 CPU sec, 4.116 real sec
sorting block 157, #reads = 1080858 mem = 536876795 peak = 1301729280
[M::process] read 675568 sequences (100000084 bp) in 1.811 CPU sec, 1.853 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286241, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.72, 121.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 20, 882)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2624)
[M::mem_pestat] mean and std.dev: (176.15, 318.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3495)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675504 sequences (100000243 bp) in 1.990 CPU sec, 1.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285956, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.98, 121.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285965, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.11, 119.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675522 reads in 353.024 CPU sec, 11.300 real sec
[M::mem_process_seqs] Processed 675568 reads in 243.760 CPU sec, 7.847 real sec
[M::mem_process_seqs] Processed 675568 reads in 253.753 CPU sec, 8.187 real sec
sorting block 158, #reads = 1080444 mem = 536879655 peak = 1301729280
sorting block 159, #reads = 1079706 mem = 536880047 peak = 1301729280
[M::process] read 675294 sequences (100000281 bp) in 2.821 CPU sec, 2.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285761, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (247.81, 125.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 1117, 1599)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4731)
[M::mem_pestat] mean and std.dev: (842.92, 730.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6297)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675504 sequences (100000094 bp) in 2.060 CPU sec, 2.061 real sec...
[M::process] read 675246 sequences (100000129 bp) in 2.010 CPU sec, 2.009 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285643, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.02, 125.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675504 reads in 238.872 CPU sec, 7.562 real sec
[M::mem_process_seqs] Processed 675294 reads in 131.250 CPU sec, 4.134 real sec
sorting block 160, #reads = 1080760 mem = 536880143 peak = 1301729280
[M::process] read 675368 sequences (100000211 bp) in 2.507 CPU sec, 2.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285425, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (248.50, 125.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675430 sequences (100000061 bp) in 2.030 CPU sec, 2.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285207, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.54, 126.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 63, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (83.40, 71.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285078, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.22, 127.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675504 reads in 351.966 CPU sec, 11.243 real sec
[M::mem_process_seqs] Processed 675246 reads in 245.353 CPU sec, 7.872 real sec
[M::mem_process_seqs] Processed 675368 reads in 254.902 CPU sec, 8.195 real sec
sorting block 161, #reads = 1080803 mem = 536879026 peak = 1301729280
sorting block 162, #reads = 1080307 mem = 536878060 peak = 1307701248
[M::process] read 675404 sequences (100000123 bp) in 2.747 CPU sec, 2.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285212, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.57, 127.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675326 sequences (100000001 bp) in 2.138 CPU sec, 2.136 real sec...
[M::process] read 675300 sequences (100000231 bp) in 2.000 CPU sec, 2.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285110, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.35, 127.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 98, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1574)
[M::mem_pestat] mean and std.dev: (136.00, 170.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2088)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675430 reads in 243.000 CPU sec, 7.692 real sec
[M::mem_process_seqs] Processed 675404 reads in 132.237 CPU sec, 4.167 real sec
sorting block 163, #reads = 1080471 mem = 536880272 peak = 1307701248
[M::process] read 675344 sequences (100000185 bp) in 2.460 CPU sec, 2.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285864, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.82, 127.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 36, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240)
[M::mem_pestat] mean and std.dev: (40.11, 37.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 308)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675318 sequences (100000038 bp) in 2.030 CPU sec, 2.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285266, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.49, 128.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285404, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (252.81, 129.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 11, 34)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 88)
[M::mem_pestat] mean and std.dev: (10.10, 9.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 115)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675326 reads in 342.454 CPU sec, 10.937 real sec
[M::mem_process_seqs] Processed 675300 reads in 235.744 CPU sec, 7.561 real sec
[M::mem_process_seqs] Processed 675344 reads in 245.156 CPU sec, 7.878 real sec
sorting block 164, #reads = 1080703 mem = 536879152 peak = 1316536320
sorting block 165, #reads = 1080532 mem = 536878629 peak = 1316536320
[M::process] read 675356 sequences (100000088 bp) in 2.035 CPU sec, 2.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285553, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (252.97, 130.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675136 sequences (100000271 bp) in 1.809 CPU sec, 1.889 real sec...
[M::process] read 675124 sequences (100000283 bp) in 1.510 CPU sec, 1.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285499, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.16, 130.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675318 reads in 241.663 CPU sec, 7.703 real sec
[M::mem_process_seqs] Processed 675356 reads in 132.120 CPU sec, 4.208 real sec
sorting block 166, #reads = 1080748 mem = 536879718 peak = 1316536320
[M::process] read 675164 sequences (100000251 bp) in 2.482 CPU sec, 2.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285713, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.00, 132.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675048 sequences (100000061 bp) in 1.730 CPU sec, 1.730 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284743, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.24, 130.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285056, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (253.86, 130.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675136 reads in 349.787 CPU sec, 11.183 real sec
[M::mem_process_seqs] Processed 675124 reads in 241.819 CPU sec, 7.770 real sec
sorting block 167, #reads = 1081008 mem = 536878471 peak = 1316536320
[M::mem_process_seqs] Processed 675164 reads in 251.463 CPU sec, 8.099 real sec
sorting block 168, #reads = 1080182 mem = 536878490 peak = 1316536320
[M::process] read 674992 sequences (100000242 bp) in 1.948 CPU sec, 2.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285234, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (254.79, 131.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 62, 875)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2581)
[M::mem_pestat] mean and std.dev: (294.70, 382.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3434)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674958 sequences (100000154 bp) in 1.946 CPU sec, 2.013 real sec...
[M::process] read 675118 sequences (100000216 bp) in 1.500 CPU sec, 1.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285220, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.26, 132.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675048 reads in 246.800 CPU sec, 7.880 real sec
[M::mem_process_seqs] Processed 674992 reads in 134.761 CPU sec, 4.298 real sec
sorting block 169, #reads = 1080536 mem = 536879734 peak = 1316536320
[M::process] read 675260 sequences (100000256 bp) in 2.478 CPU sec, 2.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285192, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.79, 132.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675078 sequences (100000252 bp) in 2.020 CPU sec, 2.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285056, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (255.84, 132.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285702, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.38, 132.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674958 reads in 343.570 CPU sec, 10.974 real sec
sorting block 170, #reads = 1080474 mem = 536877981 peak = 1316536320
[M::mem_process_seqs] Processed 675260 reads in 237.403 CPU sec, 7.619 real sec
[M::mem_process_seqs] Processed 675118 reads in 247.173 CPU sec, 7.951 real sec
sorting block 171, #reads = 1080400 mem = 536879683 peak = 1316536320
[M::process] read 675096 sequences (100000226 bp) in 2.922 CPU sec, 3.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284792, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.62, 133.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 104, 112)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314)
[M::mem_pestat] mean and std.dev: (70.50, 47.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 415)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675104 sequences (100000203 bp) in 2.070 CPU sec, 2.067 real sec...
[M::process] read 675014 sequences (100000004 bp) in 1.990 CPU sec, 1.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285391, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (253.55, 130.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 101, 101)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251)
[M::mem_pestat] mean and std.dev: (69.50, 37.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 326)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675078 reads in 244.303 CPU sec, 7.744 real sec
[M::mem_process_seqs] Processed 675096 reads in 133.405 CPU sec, 4.202 real sec
sorting block 172, #reads = 1081184 mem = 536877809 peak = 1316536320
sorting block 173, #reads = 1080547 mem = 536878521 peak = 1316536320
[M::process] read 674900 sequences (100000089 bp) in 2.528 CPU sec, 2.614 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285433, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.05, 131.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674740 sequences (100000274 bp) in 2.040 CPU sec, 2.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285218, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.13, 133.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (90, 555, 3325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9795)
[M::mem_pestat] mean and std.dev: (1147.50, 1437.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13030)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284793, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (258.85, 135.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675104 reads in 340.678 CPU sec, 10.889 real sec
[M::mem_process_seqs] Processed 675014 reads in 236.225 CPU sec, 7.563 real sec
[M::mem_process_seqs] Processed 674900 reads in 245.807 CPU sec, 7.886 real sec
sorting block 174, #reads = 1080865 mem = 536877242 peak = 1316536320
[M::process] read 674964 sequences (100000089 bp) in 2.795 CPU sec, 2.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285106, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (260.10, 137.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674962 sequences (100000084 bp) in 2.040 CPU sec, 2.033 real sec...
[M::process] read 675034 sequences (100000165 bp) in 2.010 CPU sec, 2.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284833, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (260.58, 138.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 271, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1674)
[M::mem_pestat] mean and std.dev: (372.60, 385.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2209)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674740 reads in 236.331 CPU sec, 7.459 real sec
sorting block 175, #reads = 1080566 mem = 536878877 peak = 1316536320
[M::mem_process_seqs] Processed 674964 reads in 130.776 CPU sec, 4.118 real sec
sorting block 176, #reads = 1080263 mem = 536878608 peak = 1316536320
[M::process] read 675204 sequences (100000013 bp) in 2.066 CPU sec, 2.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285339, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (259.47, 138.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676062 sequences (100000212 bp) in 1.540 CPU sec, 1.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285967, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (258.59, 137.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 87, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221)
[M::mem_pestat] mean and std.dev: (184.80, 218.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1624)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285621, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (253.59, 131.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674962 reads in 336.008 CPU sec, 10.763 real sec
[M::mem_process_seqs] Processed 675034 reads in 229.747 CPU sec, 7.406 real sec
[M::mem_process_seqs] Processed 675204 reads in 239.867 CPU sec, 7.747 real sec
sorting block 177, #reads = 1080308 mem = 536877434 peak = 1316536320
[M::process] read 676090 sequences (100000288 bp) in 2.093 CPU sec, 2.211 real sec...
sorting block 178, #reads = 1079758 mem = 536879136 peak = 1316536320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286067, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (236.92, 115.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675982 sequences (100000067 bp) in 1.769 CPU sec, 1.817 real sec...
[M::process] read 675864 sequences (100000181 bp) in 1.530 CPU sec, 1.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286349, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (238.22, 116.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (307, 1166, 2610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7216)
[M::mem_pestat] mean and std.dev: (1217.25, 934.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9519)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676062 reads in 227.868 CPU sec, 7.247 real sec
[M::mem_process_seqs] Processed 676090 reads in 126.433 CPU sec, 4.021 real sec
sorting block 179, #reads = 1080974 mem = 536878743 peak = 1316536320
[M::process] read 676102 sequences (100000126 bp) in 2.485 CPU sec, 2.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286103, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (239.18, 116.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 32, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097)
[M::mem_pestat] mean and std.dev: (187.80, 235.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1462)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675800 sequences (100000102 bp) in 2.010 CPU sec, 2.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286094, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (239.75, 117.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 104, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248)
[M::mem_pestat] mean and std.dev: (73.11, 47.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 320)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675982 reads in 222.041 CPU sec, 7.055 real sec
[M::mem_process_seqs] Processed 675864 reads in 123.877 CPU sec, 3.959 real sec
sorting block 180, #reads = 1081254 mem = 536879903 peak = 1316536320
[M::process] read 675678 sequences (100000268 bp) in 2.363 CPU sec, 2.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286522, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (240.00, 117.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675762 sequences (100000163 bp) in 2.030 CPU sec, 2.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286240, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (240.57, 118.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286143, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (244.93, 122.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 25, 1270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3784)
[M::mem_pestat] mean and std.dev: (450.50, 622.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5041)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676102 reads in 326.658 CPU sec, 10.432 real sec
[M::mem_process_seqs] Processed 675800 reads in 228.436 CPU sec, 7.315 real sec
sorting block 181, #reads = 1080974 mem = 536878329 peak = 1316536320
[M::mem_process_seqs] Processed 675678 reads in 238.145 CPU sec, 7.642 real sec
sorting block 182, #reads = 1080566 mem = 536881167 peak = 1316536320
[M::process] read 675724 sequences (100000147 bp) in 2.668 CPU sec, 2.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285935, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.06, 120.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675666 sequences (100000144 bp) in 2.175 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286260, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.73, 120.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675708 sequences (100000005 bp) in 1.980 CPU sec, 1.983 real sec...
[M::mem_process_seqs] Processed 675762 reads in 228.422 CPU sec, 7.245 real sec
[M::mem_process_seqs] Processed 675724 reads in 127.557 CPU sec, 4.009 real sec
sorting block 183, #reads = 1079565 mem = 536878612 peak = 1316536320
[M::process] read 675698 sequences (100000228 bp) in 2.480 CPU sec, 2.561 real sec...
[M::process] read 675628 sequences (100000033 bp) in 2.020 CPU sec, 2.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286158, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.41, 121.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675698 reads in 118.147 CPU sec, 3.762 real sec
sorting block 184, #reads = 1081316 mem = 536880207 peak = 1316536320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286222, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.29, 120.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675524 sequences (100000141 bp) in 2.029 CPU sec, 2.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286276, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (243.87, 121.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675666 reads in 345.891 CPU sec, 10.987 real sec
[M::mem_process_seqs] Processed 675708 reads in 245.513 CPU sec, 7.808 real sec
sorting block 185, #reads = 1080888 mem = 536876703 peak = 1316536320
[M::process] read 675536 sequences (100000049 bp) in 2.310 CPU sec, 2.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285825, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (246.00, 123.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675458 sequences (100000091 bp) in 2.030 CPU sec, 2.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286082, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (245.54, 122.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 66, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (153.90, 202.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286347, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.74, 121.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (70, 377, 1109)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3187)
[M::mem_pestat] mean and std.dev: (426.75, 530.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4226)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675628 reads in 326.166 CPU sec, 10.410 real sec
[M::mem_process_seqs] Processed 675524 reads in 224.431 CPU sec, 7.197 real sec
sorting block 186, #reads = 1081164 mem = 536880104 peak = 1316536320
[M::mem_process_seqs] Processed 675536 reads in 234.863 CPU sec, 7.556 real sec
sorting block 187, #reads = 1080804 mem = 536879455 peak = 1316536320
[M::process] read 675440 sequences (100000174 bp) in 2.070 CPU sec, 2.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286024, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (245.92, 123.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675486 sequences (100000110 bp) in 2.174 CPU sec, 2.241 real sec...
[M::process] read 675338 sequences (100000209 bp) in 1.970 CPU sec, 1.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285564, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.18, 125.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675458 reads in 234.587 CPU sec, 7.455 real sec
[M::mem_process_seqs] Processed 675440 reads in 128.880 CPU sec, 4.069 real sec
sorting block 188, #reads = 1080488 mem = 536879275 peak = 1316536320
[M::process] read 675542 sequences (100000285 bp) in 2.508 CPU sec, 2.614 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285330, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.21, 125.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675228 sequences (100000193 bp) in 2.010 CPU sec, 2.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286061, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.72, 127.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285409, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (250.93, 128.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675486 reads in 340.034 CPU sec, 10.856 real sec
[M::mem_process_seqs] Processed 675338 reads in 235.266 CPU sec, 7.542 real sec
sorting block 189, #reads = 1080484 mem = 536879883 peak = 1316536320
[M::mem_process_seqs] Processed 675542 reads in 244.866 CPU sec, 7.865 real sec
sorting block 190, #reads = 1081150 mem = 536881405 peak = 1316536320
[M::process] read 675136 sequences (100000072 bp) in 1.981 CPU sec, 2.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285651, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (251.69, 128.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675280 sequences (100000187 bp) in 2.101 CPU sec, 2.174 real sec...
[M::process] read 675254 sequences (100000031 bp) in 2.010 CPU sec, 2.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285267, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (250.82, 126.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 10, 42)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 116)
[M::mem_pestat] mean and std.dev: (16.17, 14.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 153)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675228 reads in 237.612 CPU sec, 7.550 real sec
[M::mem_process_seqs] Processed 675136 reads in 130.663 CPU sec, 4.131 real sec
sorting block 191, #reads = 1080707 mem = 536882227 peak = 1316536320
sorting block 192, #reads = 1080243 mem = 536878291 peak = 1316536320
[M::process] read 675228 sequences (100000050 bp) in 2.520 CPU sec, 2.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285469, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.12, 127.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 32, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242)
[M::mem_pestat] mean and std.dev: (36.90, 31.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 318)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675254 sequences (100000032 bp) in 2.010 CPU sec, 2.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286312, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.23, 129.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 1818, 1818)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5332)
[M::mem_pestat] mean and std.dev: (1127.30, 893.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7089)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285639, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.37, 128.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 58, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (77.25, 99.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675280 reads in 343.303 CPU sec, 10.967 real sec
[M::mem_process_seqs] Processed 675254 reads in 237.375 CPU sec, 7.623 real sec
[M::mem_process_seqs] Processed 675228 reads in 247.092 CPU sec, 7.953 real sec
sorting block 193, #reads = 1080741 mem = 536877551 peak = 1316536320
[M::process] read 675248 sequences (100000198 bp) in 2.631 CPU sec, 2.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285698, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (252.73, 129.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675214 sequences (100000122 bp) in 2.060 CPU sec, 2.057 real sec...
[M::process] read 675146 sequences (100000158 bp) in 1.990 CPU sec, 1.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285359, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.07, 129.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675254 reads in 241.805 CPU sec, 7.648 real sec
sorting block 194, #reads = 1080981 mem = 536878133 peak = 1316536320
[M::mem_process_seqs] Processed 675248 reads in 133.320 CPU sec, 4.202 real sec
sorting block 195, #reads = 1080997 mem = 536878494 peak = 1316536320
[M::process] read 675242 sequences (100000089 bp) in 2.467 CPU sec, 2.547 real sec...
[M::process] read 675204 sequences (100000169 bp) in 1.990 CPU sec, 1.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285560, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.36, 130.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675146 reads in 123.576 CPU sec, 3.949 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285363, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.45, 130.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 76, 2578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7602)
[M::mem_pestat] mean and std.dev: (987.36, 1203.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10114)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675058 sequences (100000206 bp) in 1.932 CPU sec, 1.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285308, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.05, 131.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 411, 1690)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4986)
[M::mem_pestat] mean and std.dev: (532.33, 644.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6634)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675214 reads in 358.377 CPU sec, 11.387 real sec
[M::mem_process_seqs] Processed 675242 reads in 249.541 CPU sec, 7.958 real sec
sorting block 196, #reads = 1080647 mem = 536879341 peak = 1316536320
sorting block 197, #reads = 1080272 mem = 536880064 peak = 1316536320
[M::process] read 674970 sequences (100000118 bp) in 2.521 CPU sec, 2.644 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285562, 27, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (253.68, 130.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 25, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244)
[M::mem_pestat] mean and std.dev: (32.79, 31.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 321)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674932 sequences (100000088 bp) in 2.000 CPU sec, 2.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284859, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.06, 132.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285583, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.64, 133.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675204 reads in 346.502 CPU sec, 11.067 real sec
[M::mem_process_seqs] Processed 675058 reads in 243.154 CPU sec, 7.799 real sec
[M::mem_process_seqs] Processed 674970 reads in 253.623 CPU sec, 8.154 real sec
sorting block 198, #reads = 1080532 mem = 536878141 peak = 1316536320
[M::process] read 674916 sequences (100000167 bp) in 2.116 CPU sec, 2.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285271, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.05, 132.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (386, 414, 1401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3431)
[M::mem_pestat] mean and std.dev: (784.91, 869.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4446)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674924 sequences (100000155 bp) in 2.268 CPU sec, 2.305 real sec...
[M::process] read 674958 sequences (100000102 bp) in 1.960 CPU sec, 1.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285559, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.31, 134.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 408, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1537)
[M::mem_pestat] mean and std.dev: (281.45, 234.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2038)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674932 reads in 237.789 CPU sec, 7.543 real sec
sorting block 199, #reads = 1079943 mem = 536878167 peak = 1316536320
[M::mem_process_seqs] Processed 674916 reads in 130.244 CPU sec, 4.123 real sec
sorting block 200, #reads = 1080245 mem = 536878270 peak = 1316536320
[M::process] read 675016 sequences (100000220 bp) in 2.457 CPU sec, 2.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285796, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.23, 135.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675060 sequences (100000093 bp) in 2.010 CPU sec, 2.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285106, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (258.21, 134.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285487, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (255.55, 132.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 25, 777)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2303)
[M::mem_pestat] mean and std.dev: (290.25, 332.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3066)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674924 reads in 340.955 CPU sec, 10.897 real sec
[M::mem_process_seqs] Processed 674958 reads in 236.548 CPU sec, 7.592 real sec
[M::mem_process_seqs] Processed 675016 reads in 246.245 CPU sec, 7.921 real sec
sorting block 201, #reads = 1080340 mem = 536879191 peak = 1316536320
[M::process] read 675090 sequences (100000298 bp) in 2.105 CPU sec, 2.225 real sec...
sorting block 202, #reads = 1080332 mem = 536877299 peak = 1316536320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285395, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (255.63, 132.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 241, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123)
[M::mem_pestat] mean and std.dev: (166.22, 118.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1478)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674958 sequences (100000059 bp) in 2.198 CPU sec, 2.214 real sec...
[M::process] read 674848 sequences (100000058 bp) in 2.040 CPU sec, 2.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285515, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (258.82, 135.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (84, 494, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1377)
[M::mem_pestat] mean and std.dev: (343.50, 239.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1808)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675060 reads in 241.443 CPU sec, 7.647 real sec
[M::mem_process_seqs] Processed 675090 reads in 133.389 CPU sec, 4.220 real sec
sorting block 203, #reads = 1080570 mem = 536880254 peak = 1316536320
[M::process] read 674870 sequences (100000044 bp) in 2.473 CPU sec, 2.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285685, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (260.86, 137.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 22, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1948)
[M::mem_pestat] mean and std.dev: (287.50, 442.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2594)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674872 sequences (100000255 bp) in 2.060 CPU sec, 2.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285603, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (261.29, 138.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (380, 842, 842)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1766)
[M::mem_pestat] mean and std.dev: (650.27, 278.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2228)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285541, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (261.64, 138.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 25, 32)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 46)
[M::mem_pestat] mean and std.dev: (28.43, 6.04)
[M::mem_pestat] low and high boundaries for proper pairs: (4, 53)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674958 reads in 343.470 CPU sec, 10.963 real sec
[M::mem_process_seqs] Processed 674848 reads in 238.680 CPU sec, 7.659 real sec
[M::mem_process_seqs] Processed 674870 reads in 248.451 CPU sec, 7.994 real sec
sorting block 204, #reads = 1080238 mem = 536878731 peak = 1316536320
sorting block 205, #reads = 1080297 mem = 536878616 peak = 1316536320
[M::process] read 674772 sequences (100000092 bp) in 2.439 CPU sec, 2.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285501, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (262.41, 140.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 910)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674916 sequences (100000103 bp) in 1.634 CPU sec, 1.643 real sec...
[M::process] read 676252 sequences (100000001 bp) in 1.510 CPU sec, 1.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285777, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (262.02, 139.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674872 reads in 233.697 CPU sec, 7.428 real sec
[M::mem_process_seqs] Processed 674772 reads in 129.659 CPU sec, 4.124 real sec
sorting block 206, #reads = 1079985 mem = 536879156 peak = 1316536320
[M::process] read 676074 sequences (100000292 bp) in 2.499 CPU sec, 2.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285823, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (261.08, 138.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 25, 74)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198)
[M::mem_pestat] mean and std.dev: (42.38, 49.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 260)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676064 sequences (100000103 bp) in 2.030 CPU sec, 2.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286235, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (234.78, 113.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 32, 626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1834)
[M::mem_pestat] mean and std.dev: (89.27, 173.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2438)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286331, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (237.25, 115.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (88, 95, 626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1702)
[M::mem_pestat] mean and std.dev: (287.60, 284.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2240)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674916 reads in 334.129 CPU sec, 10.666 real sec
[M::mem_process_seqs] Processed 676252 reads in 235.113 CPU sec, 7.542 real sec
[M::mem_process_seqs] Processed 676074 reads in 244.461 CPU sec, 7.858 real sec
sorting block 207, #reads = 1079515 mem = 536878361 peak = 1316536320
sorting block 208, #reads = 1080704 mem = 536879087 peak = 1316536320
[M::process] read 676062 sequences (100000087 bp) in 2.267 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286007, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (238.15, 115.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 523, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1503)
[M::mem_pestat] mean and std.dev: (300.30, 257.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1993)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675856 sequences (100000118 bp) in 2.246 CPU sec, 2.272 real sec...
[M::process] read 675890 sequences (100000136 bp) in 2.010 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286470, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (240.31, 117.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676064 reads in 229.872 CPU sec, 7.281 real sec
[M::mem_process_seqs] Processed 676062 reads in 123.631 CPU sec, 3.901 real sec
sorting block 209, #reads = 1081041 mem = 536879283 peak = 1316536320
[M::process] read 675848 sequences (100000003 bp) in 2.426 CPU sec, 2.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286344, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (240.93, 118.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675786 sequences (100000258 bp) in 2.010 CPU sec, 2.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286217, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.54, 116.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286175, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.89, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 161, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1652)
[M::mem_pestat] mean and std.dev: (266.07, 254.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2196)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675856 reads in 334.104 CPU sec, 10.666 real sec
[M::mem_process_seqs] Processed 675890 reads in 231.908 CPU sec, 7.441 real sec
[M::mem_process_seqs] Processed 675848 reads in 241.434 CPU sec, 7.764 real sec
sorting block 210, #reads = 1080681 mem = 536879936 peak = 1316536320
sorting block 211, #reads = 1080996 mem = 536880469 peak = 1316536320
[M::process] read 675728 sequences (100000009 bp) in 2.825 CPU sec, 2.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286378, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.01, 119.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 50, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1540)
[M::mem_pestat] mean and std.dev: (267.77, 428.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2045)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675828 sequences (100000082 bp) in 2.050 CPU sec, 2.047 real sec...
[M::process] read 675752 sequences (100000046 bp) in 1.990 CPU sec, 1.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286128, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.73, 119.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 954, 995)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2523)
[M::mem_pestat] mean and std.dev: (641.90, 408.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3287)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675786 reads in 234.104 CPU sec, 7.402 real sec
[M::mem_process_seqs] Processed 675728 reads in 129.352 CPU sec, 4.068 real sec
sorting block 212, #reads = 1080980 mem = 536879286 peak = 1316536320
[M::process] read 675558 sequences (100000243 bp) in 2.509 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285650, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.22, 120.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675706 sequences (100000161 bp) in 2.000 CPU sec, 2.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286008, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.85, 120.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285816, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.58, 122.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675828 reads in 334.392 CPU sec, 10.670 real sec
[M::mem_process_seqs] Processed 675752 reads in 231.093 CPU sec, 7.422 real sec
sorting block 213, #reads = 1080756 mem = 536878246 peak = 1316536320
[M::mem_process_seqs] Processed 675558 reads in 240.311 CPU sec, 7.732 real sec
sorting block 214, #reads = 1080385 mem = 536880658 peak = 1316536320
[M::process] read 675628 sequences (100000080 bp) in 2.672 CPU sec, 2.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285698, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.56, 122.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 22, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311)
[M::mem_pestat] mean and std.dev: (152.36, 200.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1745)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675468 sequences (100000239 bp) in 2.080 CPU sec, 2.084 real sec...
[M::process] read 675426 sequences (100000207 bp) in 1.990 CPU sec, 1.990 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285936, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (245.76, 122.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675706 reads in 234.730 CPU sec, 7.423 real sec
[M::mem_process_seqs] Processed 675628 reads in 128.797 CPU sec, 4.050 real sec
sorting block 215, #reads = 1080617 mem = 536877070 peak = 1316536320
[M::process] read 675544 sequences (100000013 bp) in 2.523 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285659, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (247.01, 124.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675356 sequences (100000139 bp) in 1.990 CPU sec, 1.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285764, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.56, 124.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 190, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (191.27, 192.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285644, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.11, 125.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 11, 20)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 50)
[M::mem_pestat] mean and std.dev: (10.55, 5.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 65)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675468 reads in 341.004 CPU sec, 10.889 real sec
sorting block 216, #reads = 1081161 mem = 536879861 peak = 1316536320
[M::mem_process_seqs] Processed 675426 reads in 235.938 CPU sec, 7.573 real sec
[M::mem_process_seqs] Processed 675544 reads in 245.277 CPU sec, 7.891 real sec
sorting block 217, #reads = 1080502 mem = 536878844 peak = 1316536320
[M::process] read 675160 sequences (100000067 bp) in 2.443 CPU sec, 2.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285239, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.96, 126.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675296 sequences (100000240 bp) in 2.103 CPU sec, 2.117 real sec...
[M::process] read 675240 sequences (100000147 bp) in 2.000 CPU sec, 1.995 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285288, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.51, 126.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675356 reads in 237.893 CPU sec, 7.537 real sec
sorting block 218, #reads = 1080667 mem = 536879592 peak = 1316536320
[M::mem_process_seqs] Processed 675160 reads in 130.499 CPU sec, 4.122 real sec
sorting block 219, #reads = 1080434 mem = 536881505 peak = 1316536320
[M::process] read 675214 sequences (100000232 bp) in 2.499 CPU sec, 2.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285547, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.98, 127.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 73, 1275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3775)
[M::mem_pestat] mean and std.dev: (452.00, 590.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5025)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675250 sequences (100000259 bp) in 1.990 CPU sec, 1.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285287, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.84, 128.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 32, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 189)
[M::mem_pestat] mean and std.dev: (44.50, 42.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 249)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285094, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.85, 129.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675296 reads in 346.348 CPU sec, 11.061 real sec
[M::mem_process_seqs] Processed 675240 reads in 240.106 CPU sec, 7.709 real sec
[M::mem_process_seqs] Processed 675214 reads in 250.267 CPU sec, 8.052 real sec
sorting block 220, #reads = 1080839 mem = 536879629 peak = 1316536320
sorting block 221, #reads = 1079968 mem = 536879699 peak = 1316536320
[M::process] read 675286 sequences (100000176 bp) in 2.740 CPU sec, 2.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285658, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.00, 128.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675126 sequences (100000034 bp) in 2.030 CPU sec, 2.028 real sec...
[M::process] read 675130 sequences (100000165 bp) in 1.980 CPU sec, 1.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285918, 22, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.09, 129.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 66, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165)
[M::mem_pestat] mean and std.dev: (149.33, 176.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1549)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675250 reads in 226.466 CPU sec, 7.149 real sec
[M::mem_process_seqs] Processed 675286 reads in 120.314 CPU sec, 3.788 real sec
sorting block 222, #reads = 1081037 mem = 536879304 peak = 1316536320
[M::process] read 675090 sequences (100000272 bp) in 2.594 CPU sec, 2.703 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284869, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.08, 130.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675144 sequences (100000280 bp) in 1.470 CPU sec, 1.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284998, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.74, 131.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284894, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.62, 130.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675126 reads in 325.757 CPU sec, 10.429 real sec
[M::mem_process_seqs] Processed 675130 reads in 233.600 CPU sec, 7.489 real sec
[M::mem_process_seqs] Processed 675090 reads in 243.351 CPU sec, 7.820 real sec
sorting block 223, #reads = 1080098 mem = 536878995 peak = 1316536320
sorting block 224, #reads = 1080358 mem = 536880086 peak = 1316536320
[M::process] read 675172 sequences (100000031 bp) in 2.632 CPU sec, 2.751 real sec...
[M::process] read 675072 sequences (100000185 bp) in 2.092 CPU sec, 2.137 real sec...
[M::process] read 675028 sequences (100000120 bp) in 2.000 CPU sec, 2.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284751, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.08, 130.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675172 reads in 126.444 CPU sec, 3.976 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285083, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.78, 130.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674986 sequences (100000274 bp) in 2.059 CPU sec, 2.068 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284842, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.36, 131.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 511, 1050)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2954)
[M::mem_pestat] mean and std.dev: (479.92, 357.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3906)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675144 reads in 360.996 CPU sec, 11.441 real sec
[M::mem_process_seqs] Processed 675072 reads in 141.018 CPU sec, 4.492 real sec
sorting block 225, #reads = 1080112 mem = 536876926 peak = 1316536320
sorting block 226, #reads = 1080399 mem = 536880947 peak = 1316536320
[M::process] read 674880 sequences (100000245 bp) in 2.316 CPU sec, 2.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285337, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.56, 132.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 61, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (61, 61)
[M::mem_pestat] mean and std.dev: (61.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (61, 61)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674988 sequences (100000124 bp) in 1.970 CPU sec, 1.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285084, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (258.16, 132.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285519, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.43, 132.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675028 reads in 339.147 CPU sec, 10.837 real sec
[M::mem_process_seqs] Processed 674880 reads in 230.981 CPU sec, 7.423 real sec
[M::mem_process_seqs] Processed 674986 reads in 240.043 CPU sec, 7.735 real sec
sorting block 227, #reads = 1080484 mem = 536877898 peak = 1316536320
[M::process] read 674868 sequences (100000069 bp) in 2.855 CPU sec, 3.024 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285190, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.11, 132.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 87, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2005)
[M::mem_pestat] mean and std.dev: (275.27, 304.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2667)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674860 sequences (100000287 bp) in 1.530 CPU sec, 1.523 real sec...
[M::process] read 674994 sequences (100000254 bp) in 1.450 CPU sec, 1.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284982, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.97, 133.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674988 reads in 214.239 CPU sec, 6.803 real sec
sorting block 228, #reads = 1079849 mem = 536877938 peak = 1316536320
[M::mem_process_seqs] Processed 674868 reads in 117.301 CPU sec, 3.714 real sec
sorting block 229, #reads = 1080375 mem = 536879349 peak = 1316536320
[M::process] read 674974 sequences (100000268 bp) in 2.148 CPU sec, 2.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285905, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.66, 135.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 17, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (81.62, 114.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674944 sequences (100000044 bp) in 1.450 CPU sec, 1.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284979, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.64, 134.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 36, 36)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 86)
[M::mem_pestat] mean and std.dev: (28.83, 22.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 117)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674860 reads in 203.655 CPU sec, 6.495 real sec
[M::mem_process_seqs] Processed 674994 reads in 112.732 CPU sec, 3.628 real sec
sorting block 230, #reads = 1080967 mem = 536878234 peak = 1316536320
[M::process] read 674952 sequences (100000093 bp) in 1.823 CPU sec, 1.841 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285456, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.96, 135.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 183, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (171, 201)
[M::mem_pestat] mean and std.dev: (185.57, 3.50)
[M::mem_pestat] low and high boundaries for proper pairs: (165, 207)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674806 sequences (100000027 bp) in 1.490 CPU sec, 1.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285466, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (261.32, 136.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285433, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (261.19, 136.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 240, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (121.08, 124.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674974 reads in 291.825 CPU sec, 9.354 real sec
[M::mem_process_seqs] Processed 674944 reads in 203.530 CPU sec, 6.552 real sec
[M::mem_process_seqs] Processed 674952 reads in 212.622 CPU sec, 6.867 real sec
sorting block 231, #reads = 1080416 mem = 536878146 peak = 1316536320
sorting block 232, #reads = 1080424 mem = 536878810 peak = 1316536320
[M::process] read 674780 sequences (100000083 bp) in 2.031 CPU sec, 2.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285272, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.39, 137.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674758 sequences (100000233 bp) in 1.656 CPU sec, 1.672 real sec...
[M::process] read 674848 sequences (100000255 bp) in 1.450 CPU sec, 1.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285501, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (262.73, 138.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674806 reads in 201.429 CPU sec, 6.405 real sec
[M::mem_process_seqs] Processed 674780 reads in 112.842 CPU sec, 3.593 real sec
sorting block 233, #reads = 1080590 mem = 536879871 peak = 1316536320
[M::process] read 674766 sequences (100000167 bp) in 2.422 CPU sec, 2.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285047, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (262.36, 138.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674720 sequences (100000141 bp) in 1.490 CPU sec, 1.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285256, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (262.97, 139.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 421, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262)
[M::mem_pestat] mean and std.dev: (277.90, 194.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1666)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674758 reads in 197.813 CPU sec, 6.311 real sec
[M::mem_process_seqs] Processed 674848 reads in 109.472 CPU sec, 3.507 real sec
sorting block 234, #reads = 1080224 mem = 536879972 peak = 1316536320
[M::process] read 290420 sequences (43033717 bp) in 0.930 CPU sec, 0.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285449, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (262.26, 138.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 123119, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (263.95, 141.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285634, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (261.64, 138.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 193, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (103.00, 127.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1410)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674766 reads in 228.042 CPU sec, 7.374 real sec
[M::mem_process_seqs] Processed 290420 reads in 139.210 CPU sec, 4.504 real sec
sorting block 235, #reads = 1080327 mem = 536877926 peak = 1316536320
[M::mem_process_seqs] Processed 674720 reads in 148.337 CPU sec, 4.820 real sec
sorting block 236, #reads = 1080145 mem = 536877759 peak = 1316536320
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 254171732
overall: 70124216320 mem 43795.580 user 710.766 sys 1453.841 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003322.L007\tPU:L007\tSM:T21-D0003322\tLB:T21-D0003322\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 T21-D0003322_R1.fastq.gz T21-D0003322_R2.fastq.gz
[main] Real time: 1453.879 sec; CPU: 44506.350 sec
sorting block 237, #reads = 492679 mem = 245002086 peak = 1316536320
uniq header count 1
merging 238 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31 max 96
algo: util-sort
license: sentieon:util=1
output file size: 8585310855
output reads: 256588482
bam_mem_sort: 238 calls 243.071 user 4.376 sys 247.686 real
bam_write: 238 calls 70.184 user 3.420 sys 73.157 real
execute: 1 calls 70.696 user 2.223 sys 1529.862 real
merge_files: 1 calls 70.675 user 2.178 sys 75.622 real
parse_chunk: 3800 calls 296.682 user 57.943 sys 358.347 real
read_chunk: 10226 calls 113.381 user 73.074 sys 1421.568 real
sort_block: 1 calls 76.007 user 72.524 sys 1376.356 real
write_chunk: 6424 calls 69.926 user 1.787 sys 72.420 real
overall: 3051274240 mem 3257.400 user 176.022 sys 1529.886 real
ls: cannot access '*.cram': No such file or directory