File Info

Filename
.command.log
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/21/c07c93438085a32ee4738d1f03fceb/.command.log
Size
122.4 KB
Task
DAQ:SENTIEON_BWAMEM (T18-D0003255;L002;0003)
Attempt

Content

View in new tab 
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/21/c07c93438085a32ee4738d1f03fceb/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1c/e8841f10be58310658995c33a27bc4/T18-D0003255/0003.T18-D0003255_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1c/e8841f10be58310658995c33a27bc4/T18-D0003255/0003.T18-D0003255_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/21/c07c93438085a32ee4738d1f03fceb/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 2037.565 CPU sec, 487.688 real sec
[M::process] read 673418 sequences (100000003 bp) in 1.242 CPU sec, 1.245 real sec...
[M::process] read 673230 sequences (100000033 bp) in 1.463 CPU sec, 1.537 real sec...
[M::process] read 673120 sequences (100000151 bp) in 1.472 CPU sec, 1.528 real sec...
[M::process] read 673168 sequences (100000090 bp) in 1.237 CPU sec, 1.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285366, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (261.88, 129.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285782, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (266.12, 133.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 39, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1949)
[M::mem_pestat] mean and std.dev: (359.13, 588.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2712)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285984, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.39, 136.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 65, 94)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 220)
[M::mem_pestat] mean and std.dev: (47.60, 27.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 283)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673418 reads in 336.623 CPU sec, 10.935 real sec
[M::mem_process_seqs] Processed 673230 reads in 211.243 CPU sec, 6.900 real sec
[M::mem_process_seqs] Processed 673120 reads in 219.328 CPU sec, 7.185 real sec
sorting block 0, #reads = 1079776 mem = 536878471 peak = 588333056
[M::process] read 673034 sequences (100000032 bp) in 1.884 CPU sec, 2.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285468, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.94, 137.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673046 sequences (100000075 bp) in 1.623 CPU sec, 1.693 real sec...
[M::process] read 672900 sequences (100000224 bp) in 1.384 CPU sec, 1.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285663, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.88, 139.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 98, 98)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 228)
[M::mem_pestat] mean and std.dev: (57.38, 32.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 293)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673168 reads in 210.763 CPU sec, 6.732 real sec
[M::mem_process_seqs] Processed 673034 reads in 113.551 CPU sec, 3.624 real sec
sorting block 1, #reads = 1079975 mem = 536880292 peak = 887177216
sorting block 2, #reads = 1079761 mem = 536880703 peak = 887177216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285049, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.11, 141.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 187, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (160.22, 141.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1378)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672748 sequences (100000188 bp) in 2.537 CPU sec, 2.713 real sec...
[M::process] read 672694 sequences (100000093 bp) in 1.329 CPU sec, 1.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284227, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.84, 141.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673046 reads in 217.366 CPU sec, 6.981 real sec
[M::mem_process_seqs] Processed 672900 reads in 122.049 CPU sec, 3.905 real sec
sorting block 3, #reads = 1079499 mem = 536881097 peak = 887177216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284667, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.71, 145.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 17, 35)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 81)
[M::mem_pestat] mean and std.dev: (17.36, 11.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 104)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672660 sequences (100000149 bp) in 2.220 CPU sec, 2.411 real sec...
[M::process] read 672652 sequences (100000154 bp) in 1.246 CPU sec, 1.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284574, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.63, 145.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 15, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (63.67, 122.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672748 reads in 223.029 CPU sec, 7.181 real sec
[M::mem_process_seqs] Processed 672694 reads in 123.648 CPU sec, 3.973 real sec
sorting block 4, #reads = 1079105 mem = 536879911 peak = 887177216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284128, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (282.98, 147.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672688 sequences (100000294 bp) in 2.123 CPU sec, 2.298 real sec...
[M::process] read 672870 sequences (100000250 bp) in 1.476 CPU sec, 1.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284521, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (285.62, 149.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 346, 2830)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8142)
[M::mem_pestat] mean and std.dev: (1123.88, 1407.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10798)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672660 reads in 223.969 CPU sec, 7.200 real sec
[M::mem_process_seqs] Processed 672652 reads in 124.679 CPU sec, 4.001 real sec
sorting block 5, #reads = 1079347 mem = 536880371 peak = 887177216
[M::process] read 672856 sequences (100000131 bp) in 2.030 CPU sec, 2.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284224, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.55, 148.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672718 sequences (100000155 bp) in 1.361 CPU sec, 1.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284574, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.80, 144.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284505, 21, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.83, 144.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 199, 1963)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5837)
[M::mem_pestat] mean and std.dev: (952.29, 923.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7774)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672688 reads in 325.093 CPU sec, 10.489 real sec
sorting block 6, #reads = 1078896 mem = 536875575 peak = 887177216
[M::mem_process_seqs] Processed 672856 reads in 223.399 CPU sec, 7.210 real sec
[M::mem_process_seqs] Processed 672870 reads in 231.875 CPU sec, 7.504 real sec
sorting block 7, #reads = 1079289 mem = 536878111 peak = 887177216
[M::process] read 672678 sequences (100000060 bp) in 2.207 CPU sec, 2.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284658, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.26, 144.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 29, 801)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2397)
[M::mem_pestat] mean and std.dev: (327.15, 465.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3195)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672966 sequences (100000263 bp) in 2.076 CPU sec, 2.219 real sec...
[M::process] read 673252 sequences (100000196 bp) in 1.492 CPU sec, 1.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285009, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.98, 146.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672718 reads in 223.732 CPU sec, 7.191 real sec
[M::mem_process_seqs] Processed 672678 reads in 122.587 CPU sec, 3.910 real sec
sorting block 8, #reads = 1079337 mem = 536879315 peak = 887177216
sorting block 9, #reads = 1079714 mem = 536877193 peak = 887177216
[M::process] read 673268 sequences (100000288 bp) in 2.085 CPU sec, 2.268 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285780, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.70, 141.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 10, 29)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 71)
[M::mem_pestat] mean and std.dev: (9.56, 7.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 92)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673050 sequences (100000216 bp) in 1.212 CPU sec, 1.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285887, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.22, 137.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (81, 196, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (144.07, 130.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285824, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (266.20, 133.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 44, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (53.30, 63.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672966 reads in 305.341 CPU sec, 9.827 real sec
[M::mem_process_seqs] Processed 673268 reads in 212.219 CPU sec, 6.836 real sec
[M::mem_process_seqs] Processed 673252 reads in 220.467 CPU sec, 7.129 real sec
sorting block 10, #reads = 1080234 mem = 536878879 peak = 887177216
[M::process] read 673078 sequences (100000044 bp) in 2.340 CPU sec, 2.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285603, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.54, 140.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (96, 486, 1201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3411)
[M::mem_pestat] mean and std.dev: (327.75, 379.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4516)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673024 sequences (100000027 bp) in 1.760 CPU sec, 1.836 real sec...
[M::process] read 673062 sequences (100000145 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285176, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.37, 142.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673050 reads in 212.770 CPU sec, 6.811 real sec
sorting block 11, #reads = 1079709 mem = 536879147 peak = 887177216
[M::process] read 672678 sequences (100000231 bp) in 1.097 CPU sec, 1.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285558, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (274.65, 142.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673024 reads in 109.635 CPU sec, 3.609 real sec
sorting block 12, #reads = 1080414 mem = 536879060 peak = 887177216
[M::mem_process_seqs] Processed 673078 reads in 252.419 CPU sec, 8.166 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284926, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.58, 144.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672708 sequences (100000261 bp) in 2.455 CPU sec, 2.678 real sec...
[M::process] read 672714 sequences (100000280 bp) in 1.549 CPU sec, 1.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284835, 19, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.40, 147.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 122, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (154.67, 102.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673062 reads in 226.452 CPU sec, 7.242 real sec
[M::mem_process_seqs] Processed 672678 reads in 119.105 CPU sec, 3.809 real sec
sorting block 13, #reads = 1079668 mem = 536879257 peak = 887177216
sorting block 14, #reads = 1079593 mem = 536875313 peak = 887177216
[M::process] read 672514 sequences (100000126 bp) in 1.949 CPU sec, 2.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284623, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (285.41, 149.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 81, 177)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (89.33, 123.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 675)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672480 sequences (100000263 bp) in 1.023 CPU sec, 1.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284948, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (285.21, 149.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284027, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 210, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (287.96, 151.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672708 reads in 319.583 CPU sec, 10.307 real sec
[M::mem_process_seqs] Processed 672714 reads in 219.974 CPU sec, 7.102 real sec
[M::mem_process_seqs] Processed 672514 reads in 228.413 CPU sec, 7.403 real sec
sorting block 15, #reads = 1079823 mem = 536880951 peak = 887177216
[M::process] read 672960 sequences (100000036 bp) in 2.277 CPU sec, 2.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283949, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (288.37, 152.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672932 sequences (100000054 bp) in 1.564 CPU sec, 1.659 real sec...
[M::process] read 672876 sequences (100000289 bp) in 1.104 CPU sec, 1.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284871, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.21, 144.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672480 reads in 223.983 CPU sec, 7.189 real sec
sorting block 16, #reads = 1078961 mem = 536877189 peak = 887177216
[M::mem_process_seqs] Processed 672960 reads in 121.341 CPU sec, 3.890 real sec
sorting block 17, #reads = 1079485 mem = 536876968 peak = 887177216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284899, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.14, 146.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672770 sequences (100000134 bp) in 2.911 CPU sec, 3.247 real sec...
[M::process] read 672592 sequences (100000299 bp) in 1.490 CPU sec, 1.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284784, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.67, 146.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672932 reads in 217.996 CPU sec, 6.971 real sec
[M::mem_process_seqs] Processed 672876 reads in 120.510 CPU sec, 3.848 real sec
sorting block 18, #reads = 1079894 mem = 536881375 peak = 887177216
[M::process] read 673364 sequences (100000163 bp) in 1.954 CPU sec, 2.092 real sec...
[M::process] read 673174 sequences (100000247 bp) in 1.656 CPU sec, 1.664 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284606, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (285.20, 149.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672592 reads in 108.810 CPU sec, 3.481 real sec
sorting block 19, #reads = 1079196 mem = 536879451 peak = 887177216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284954, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (283.23, 149.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673106 sequences (100000043 bp) in 1.922 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285393, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (268.11, 135.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672770 reads in 318.845 CPU sec, 10.179 real sec
[M::mem_process_seqs] Processed 673364 reads in 222.737 CPU sec, 7.116 real sec
sorting block 20, #reads = 1079524 mem = 536878720 peak = 887177216
[M::process] read 673044 sequences (100000059 bp) in 2.231 CPU sec, 2.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285685, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (268.21, 134.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 86, 128)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290)
[M::mem_pestat] mean and std.dev: (78.55, 61.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 371)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673146 sequences (100000148 bp) in 1.451 CPU sec, 1.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285094, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (271.19, 137.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285853, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.74, 139.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673174 reads in 307.361 CPU sec, 9.856 real sec
[M::mem_process_seqs] Processed 673106 reads in 213.573 CPU sec, 6.878 real sec
[M::mem_process_seqs] Processed 673044 reads in 221.770 CPU sec, 7.165 real sec
sorting block 21, #reads = 1079790 mem = 536877644 peak = 887177216
[M::process] read 672970 sequences (100000236 bp) in 2.658 CPU sec, 2.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284986, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.23, 142.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 76, 734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2138)
[M::mem_pestat] mean and std.dev: (242.25, 316.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2840)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 22, #reads = 1079587 mem = 536878326 peak = 887177216
[M::process] read 672916 sequences (100000106 bp) in 2.436 CPU sec, 2.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285011, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.56, 143.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673146 reads in 217.857 CPU sec, 6.963 real sec
[M::mem_process_seqs] Processed 672970 reads in 121.710 CPU sec, 3.854 real sec
[M::process] read 672726 sequences (100000265 bp) in 1.719 CPU sec, 1.760 real sec...
sorting block 23, #reads = 1080199 mem = 536878234 peak = 887177216
[M::process] read 672720 sequences (100000291 bp) in 2.146 CPU sec, 2.279 real sec...
[M::process] read 672688 sequences (100000261 bp) in 1.498 CPU sec, 1.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284593, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (283.73, 147.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672726 reads in 112.620 CPU sec, 3.602 real sec
sorting block 24, #reads = 1079609 mem = 536876316 peak = 887177216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284107, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.72, 145.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (94, 400, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108)
[M::mem_pestat] mean and std.dev: (285.70, 205.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1446)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672546 sequences (100000207 bp) in 1.338 CPU sec, 1.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284097, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (284.21, 148.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672916 reads in 334.104 CPU sec, 10.704 real sec
[M::mem_process_seqs] Processed 672720 reads in 238.241 CPU sec, 7.649 real sec
sorting block 25, #reads = 1079513 mem = 536876315 peak = 887177216
[M::process] read 672818 sequences (100000281 bp) in 2.623 CPU sec, 2.867 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284065, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (284.23, 148.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672974 sequences (100000275 bp) in 1.562 CPU sec, 1.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284140, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (286.49, 150.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672688 reads in 227.033 CPU sec, 7.273 real sec
[M::mem_process_seqs] Processed 672546 reads in 124.705 CPU sec, 4.004 real sec
sorting block 26, #reads = 1079064 mem = 536878388 peak = 887177216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284037, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.88, 145.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672816 sequences (100000183 bp) in 3.173 CPU sec, 3.480 real sec...
[M::process] read 672808 sequences (100000195 bp) in 1.880 CPU sec, 1.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284832, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.07, 144.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 98, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125)
[M::mem_pestat] mean and std.dev: (112.11, 149.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1498)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672818 reads in 219.324 CPU sec, 7.003 real sec
[M::mem_process_seqs] Processed 672974 reads in 122.581 CPU sec, 3.906 real sec
sorting block 27, #reads = 1079071 mem = 536880095 peak = 887177216
[M::process] read 672716 sequences (100000011 bp) in 2.337 CPU sec, 2.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284318, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.15, 145.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 380, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (234.17, 199.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1416)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672870 sequences (100000253 bp) in 1.249 CPU sec, 1.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284984, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (284.08, 149.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283899, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.09, 146.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 754, 1533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4541)
[M::mem_pestat] mean and std.dev: (810.93, 783.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6045)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672816 reads in 304.396 CPU sec, 9.761 real sec
[M::mem_process_seqs] Processed 672716 reads in 204.841 CPU sec, 6.614 real sec
sorting block 28, #reads = 1079633 mem = 536878902 peak = 887177216
[M::mem_process_seqs] Processed 672808 reads in 213.574 CPU sec, 6.918 real sec
sorting block 29, #reads = 1079564 mem = 536878193 peak = 887177216
[M::process] read 673340 sequences (100000240 bp) in 2.535 CPU sec, 2.760 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284987, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.23, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 269, 1030)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2834)
[M::mem_pestat] mean and std.dev: (737.27, 941.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4503)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673112 sequences (100000220 bp) in 1.490 CPU sec, 1.501 real sec...
[M::process] read 673058 sequences (100000146 bp) in 1.450 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285670, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.98, 133.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672870 reads in 194.243 CPU sec, 6.204 real sec
[M::mem_process_seqs] Processed 673340 reads in 106.458 CPU sec, 3.373 real sec
sorting block 30, #reads = 1079153 mem = 536877488 peak = 887177216
sorting block 31, #reads = 1079873 mem = 536880184 peak = 887177216
[M::process] read 672944 sequences (100000186 bp) in 2.083 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285195, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (268.42, 134.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 23, 75)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 219)
[M::mem_pestat] mean and std.dev: (26.40, 32.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 291)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672900 sequences (100000092 bp) in 1.122 CPU sec, 1.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284784, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (270.67, 136.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284793, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.91, 138.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673112 reads in 295.829 CPU sec, 9.506 real sec
[M::mem_process_seqs] Processed 673058 reads in 203.202 CPU sec, 6.543 real sec
[M::mem_process_seqs] Processed 672944 reads in 211.032 CPU sec, 6.815 real sec
sorting block 32, #reads = 1079089 mem = 536879013 peak = 887177216
[M::process] read 672968 sequences (100000266 bp) in 2.468 CPU sec, 2.741 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284973, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.78, 142.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672688 sequences (100000020 bp) in 2.036 CPU sec, 2.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284808, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.09, 143.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 180, 668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1800)
[M::mem_pestat] mean and std.dev: (265.17, 248.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2366)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672738 sequences (100000282 bp) in 1.510 CPU sec, 1.510 real sec...
[M::mem_process_seqs] Processed 672900 reads in 205.420 CPU sec, 6.569 real sec
sorting block 33, #reads = 1079433 mem = 536878134 peak = 887177216
[M::process] read 672670 sequences (100000265 bp) in 1.445 CPU sec, 1.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284515, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.23, 145.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 73, 1956)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5790)
[M::mem_pestat] mean and std.dev: (784.00, 1388.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7707)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672688 reads in 113.946 CPU sec, 3.658 real sec
sorting block 34, #reads = 1079513 mem = 536878943 peak = 887177216
[M::mem_process_seqs] Processed 672968 reads in 254.428 CPU sec, 8.118 real sec
[M::process] read 672648 sequences (100000160 bp) in 2.263 CPU sec, 2.486 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284195, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.59, 146.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672846 sequences (100000229 bp) in 1.198 CPU sec, 1.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283868, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (281.21, 145.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 400, 591)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1703)
[M::mem_pestat] mean and std.dev: (402.90, 418.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2259)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672738 reads in 227.914 CPU sec, 7.287 real sec
[M::mem_process_seqs] Processed 672670 reads in 120.481 CPU sec, 3.868 real sec
sorting block 35, #reads = 1079346 mem = 536877153 peak = 887177216
sorting block 36, #reads = 1079197 mem = 536877968 peak = 887177216
[M::process] read 672940 sequences (100000153 bp) in 1.930 CPU sec, 2.023 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284001, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (282.88, 146.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 15, 793)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2351)
[M::mem_pestat] mean and std.dev: (262.30, 350.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3130)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672914 sequences (100000093 bp) in 1.490 CPU sec, 1.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284265, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.58, 141.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284438, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.94, 145.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672648 reads in 315.064 CPU sec, 10.110 real sec
[M::mem_process_seqs] Processed 672940 reads in 217.562 CPU sec, 7.020 real sec
[M::mem_process_seqs] Processed 672846 reads in 225.985 CPU sec, 7.314 real sec
sorting block 37, #reads = 1079131 mem = 536878424 peak = 887177216
[M::process] read 672736 sequences (100000009 bp) in 2.329 CPU sec, 2.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284211, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.59, 142.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672758 sequences (100000001 bp) in 1.895 CPU sec, 1.971 real sec...
[M::process] read 672596 sequences (100000039 bp) in 1.490 CPU sec, 1.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284300, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.27, 143.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 55, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1753)
[M::mem_pestat] mean and std.dev: (177.38, 266.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2331)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672914 reads in 213.472 CPU sec, 6.821 real sec
sorting block 38, #reads = 1079107 mem = 536880957 peak = 887177216
[M::process] read 673352 sequences (100000042 bp) in 1.091 CPU sec, 1.094 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284881, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.33, 146.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 224, 1086)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3240)
[M::mem_pestat] mean and std.dev: (592.46, 696.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4317)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672758 reads in 107.444 CPU sec, 3.539 real sec
sorting block 39, #reads = 1079455 mem = 536879732 peak = 887177216
[M::mem_process_seqs] Processed 672736 reads in 244.962 CPU sec, 7.929 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284664, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.68, 148.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 93, 96)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 116)
[M::mem_pestat] mean and std.dev: (92.83, 3.34)
[M::mem_pestat] low and high boundaries for proper pairs: (56, 126)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673454 sequences (100000292 bp) in 2.562 CPU sec, 2.807 real sec...
[M::process] read 673220 sequences (100000116 bp) in 1.435 CPU sec, 1.444 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285236, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.97, 142.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672596 reads in 212.033 CPU sec, 6.761 real sec
[M::mem_process_seqs] Processed 673352 reads in 113.037 CPU sec, 3.612 real sec
sorting block 40, #reads = 1079898 mem = 536877250 peak = 887177216
sorting block 41, #reads = 1079641 mem = 536880640 peak = 887177216
[M::process] read 673158 sequences (100000065 bp) in 2.103 CPU sec, 2.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284346, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.89, 139.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673262 sequences (100000221 bp) in 1.550 CPU sec, 1.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284167, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.86, 140.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283828, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.62, 141.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 224, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184)
[M::mem_pestat] mean and std.dev: (256.00, 261.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1568)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673454 reads in 304.829 CPU sec, 9.778 real sec
[M::mem_process_seqs] Processed 673220 reads in 211.346 CPU sec, 6.810 real sec
[M::mem_process_seqs] Processed 673158 reads in 219.710 CPU sec, 7.104 real sec
sorting block 42, #reads = 1079272 mem = 536879143 peak = 887177216
[M::process] read 673088 sequences (100000163 bp) in 1.877 CPU sec, 2.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284478, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.27, 140.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 24, 35)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 57)
[M::mem_pestat] mean and std.dev: (24.70, 10.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 68)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673026 sequences (100000295 bp) in 1.809 CPU sec, 1.885 real sec...
[M::process] read 672866 sequences (100000037 bp) in 1.559 CPU sec, 1.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284479, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.94, 141.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 681, 1496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4030)
[M::mem_pestat] mean and std.dev: (691.82, 563.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5297)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673262 reads in 214.155 CPU sec, 6.826 real sec
sorting block 43, #reads = 1079459 mem = 536878595 peak = 887177216
[M::mem_process_seqs] Processed 673088 reads in 116.313 CPU sec, 3.697 real sec
sorting block 44, #reads = 1079620 mem = 536880184 peak = 887177216
[M::process] read 672814 sequences (100000211 bp) in 2.297 CPU sec, 2.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284041, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (275.37, 144.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672750 sequences (100000049 bp) in 1.240 CPU sec, 1.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283661, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.87, 149.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283794, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.54, 148.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 196, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617)
[M::mem_pestat] mean and std.dev: (154.18, 157.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673026 reads in 293.581 CPU sec, 9.410 real sec
[M::mem_process_seqs] Processed 672814 reads in 204.246 CPU sec, 6.571 real sec
[M::mem_process_seqs] Processed 672866 reads in 211.608 CPU sec, 6.825 real sec
sorting block 45, #reads = 1078165 mem = 536876989 peak = 887177216
[M::process] read 672738 sequences (100000010 bp) in 2.559 CPU sec, 2.878 real sec...
sorting block 46, #reads = 1078326 mem = 536876610 peak = 887177216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283675, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.50, 150.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672888 sequences (100000095 bp) in 1.712 CPU sec, 1.727 real sec...
[M::process] read 673094 sequences (100000041 bp) in 1.589 CPU sec, 1.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282923, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (286.50, 154.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 54, 106)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240)
[M::mem_pestat] mean and std.dev: (49.62, 31.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 307)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672750 reads in 216.315 CPU sec, 6.906 real sec
[M::mem_process_seqs] Processed 672738 reads in 121.175 CPU sec, 3.835 real sec
sorting block 47, #reads = 1078507 mem = 536879892 peak = 887177216
[M::process] read 672988 sequences (100000127 bp) in 2.403 CPU sec, 2.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283743, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (278.70, 147.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 68, 114)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334)
[M::mem_pestat] mean and std.dev: (49.27, 46.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673110 sequences (100000147 bp) in 1.600 CPU sec, 1.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283785, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.69, 144.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672888 reads in 218.806 CPU sec, 6.987 real sec
[M::mem_process_seqs] Processed 673094 reads in 118.833 CPU sec, 3.812 real sec
sorting block 48, #reads = 1078636 mem = 536879256 peak = 887177216
[M::process] read 672970 sequences (100000238 bp) in 1.922 CPU sec, 2.020 real sec...
[M::process] read 673378 sequences (100000148 bp) in 1.590 CPU sec, 1.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283881, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (278.88, 149.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672970 reads in 103.908 CPU sec, 3.332 real sec
sorting block 49, #reads = 1079689 mem = 536879217 peak = 887177216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284051, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.72, 144.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673294 sequences (100000165 bp) in 1.744 CPU sec, 1.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284594, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.48, 144.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672988 reads in 309.293 CPU sec, 9.870 real sec
[M::mem_process_seqs] Processed 673110 reads in 214.704 CPU sec, 6.870 real sec
sorting block 50, #reads = 1079411 mem = 536878743 peak = 887177216
[M::process] read 673162 sequences (100000239 bp) in 1.991 CPU sec, 2.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285276, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (268.95, 141.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673118 sequences (100000098 bp) in 1.560 CPU sec, 1.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285156, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.30, 139.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 21, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280)
[M::mem_pestat] mean and std.dev: (26.33, 29.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 369)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284811, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.00, 140.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673378 reads in 280.847 CPU sec, 9.000 real sec
[M::mem_process_seqs] Processed 673294 reads in 190.962 CPU sec, 6.139 real sec
[M::mem_process_seqs] Processed 673162 reads in 198.461 CPU sec, 6.400 real sec
sorting block 51, #reads = 1079364 mem = 536880189 peak = 887177216
sorting block 52, #reads = 1079479 mem = 536881071 peak = 887177216
[M::process] read 673214 sequences (100000027 bp) in 2.495 CPU sec, 2.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284428, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.87, 141.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673132 sequences (100000198 bp) in 1.678 CPU sec, 1.713 real sec...
[M::process] read 673038 sequences (100000206 bp) in 1.270 CPU sec, 1.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284317, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.81, 141.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673118 reads in 194.659 CPU sec, 6.206 real sec
[M::mem_process_seqs] Processed 673214 reads in 103.870 CPU sec, 3.287 real sec
sorting block 53, #reads = 1078035 mem = 536880504 peak = 887177216
[M::process] read 672864 sequences (100000103 bp) in 2.018 CPU sec, 2.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284745, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.44, 142.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672806 sequences (100000239 bp) in 1.160 CPU sec, 1.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284758, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (275.63, 144.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 3366, 3366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10018)
[M::mem_pestat] mean and std.dev: (1951.00, 1786.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13344)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283984, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (281.85, 149.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673132 reads in 271.132 CPU sec, 8.699 real sec
[M::mem_process_seqs] Processed 673038 reads in 189.514 CPU sec, 6.103 real sec
[M::mem_process_seqs] Processed 672864 reads in 196.804 CPU sec, 6.360 real sec
sorting block 54, #reads = 1076918 mem = 536880303 peak = 887177216
sorting block 55, #reads = 1077257 mem = 536879331 peak = 887177216
[M::process] read 672738 sequences (100000168 bp) in 2.224 CPU sec, 2.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283892, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (284.20, 152.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672800 sequences (100000253 bp) in 1.540 CPU sec, 1.624 real sec...
[M::process] read 673184 sequences (100000149 bp) in 1.139 CPU sec, 1.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284334, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (284.86, 153.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 36, 169)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435)
[M::mem_pestat] mean and std.dev: (114.80, 136.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 662)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672806 reads in 209.123 CPU sec, 6.683 real sec
[M::mem_process_seqs] Processed 672738 reads in 115.206 CPU sec, 3.673 real sec
sorting block 56, #reads = 1079019 mem = 536879340 peak = 887177216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283530, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (287.68, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 25, 94)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254)
[M::mem_pestat] mean and std.dev: (40.64, 47.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 334)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673112 sequences (100000198 bp) in 2.751 CPU sec, 3.042 real sec...
[M::process] read 673050 sequences (100000098 bp) in 1.190 CPU sec, 1.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283875, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (276.22, 147.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672800 reads in 195.922 CPU sec, 6.275 real sec
[M::mem_process_seqs] Processed 673184 reads in 104.350 CPU sec, 3.327 real sec
sorting block 57, #reads = 1078582 mem = 536878277 peak = 887177216
sorting block 58, #reads = 1079094 mem = 536879129 peak = 887463936
[M::process] read 673264 sequences (100000093 bp) in 1.789 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284078, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.62, 146.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 194, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522)
[M::mem_pestat] mean and std.dev: (121.22, 82.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673088 sequences (100000051 bp) in 1.510 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284837, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.39, 146.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284550, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (272.57, 146.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673112 reads in 271.024 CPU sec, 8.686 real sec
[M::mem_process_seqs] Processed 673050 reads in 184.211 CPU sec, 5.940 real sec
[M::mem_process_seqs] Processed 673264 reads in 191.375 CPU sec, 6.192 real sec
sorting block 59, #reads = 1079236 mem = 536879975 peak = 887463936
[M::process] read 673552 sequences (100000249 bp) in 2.377 CPU sec, 2.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284473, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (277.32, 151.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (618, 1373, 3210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8394)
[M::mem_pestat] mean and std.dev: (1542.00, 1146.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10986)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673212 sequences (100000071 bp) in 1.194 CPU sec, 1.200 real sec...
[M::process] read 673160 sequences (100000027 bp) in 1.080 CPU sec, 1.078 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285264, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (266.72, 138.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673088 reads in 178.478 CPU sec, 5.699 real sec
sorting block 60, #reads = 1078379 mem = 536880776 peak = 887463936
[M::mem_process_seqs] Processed 673552 reads in 100.254 CPU sec, 3.187 real sec
sorting block 61, #reads = 1078588 mem = 536878794 peak = 887463936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285009, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 673172 sequences (100000296 bp) in 2.038 CPU sec, 2.207 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.86, 141.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673126 sequences (100000121 bp) in 1.560 CPU sec, 1.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285012, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.48, 141.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 41, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193)
[M::mem_pestat] mean and std.dev: (190.57, 321.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1577)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673212 reads in 180.440 CPU sec, 5.759 real sec
[M::mem_process_seqs] Processed 673160 reads in 99.814 CPU sec, 3.194 real sec
sorting block 62, #reads = 1079918 mem = 536878529 peak = 887463936
[M::process] read 673022 sequences (100000046 bp) in 1.951 CPU sec, 2.036 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284552, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.40, 144.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (363, 713, 4412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12510)
[M::mem_pestat] mean and std.dev: (1522.25, 1908.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16559)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673048 sequences (100000150 bp) in 1.280 CPU sec, 1.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285003, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (275.46, 144.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284409, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.90, 144.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673172 reads in 258.670 CPU sec, 8.291 real sec
[M::mem_process_seqs] Processed 673022 reads in 176.632 CPU sec, 5.682 real sec
[M::mem_process_seqs] Processed 673126 reads in 183.746 CPU sec, 5.930 real sec
sorting block 63, #reads = 1078424 mem = 536879524 peak = 887463936
sorting block 64, #reads = 1077729 mem = 536879607 peak = 887463936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283985, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.17, 149.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 122, 122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338)
[M::mem_pestat] mean and std.dev: (91.67, 59.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 446)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672954 sequences (100000110 bp) in 2.769 CPU sec, 2.991 real sec...
[M::mem_process_seqs] Processed 673048 reads in 98.762 CPU sec, 3.245 real sec
[M::process] read 672832 sequences (100000031 bp) in 2.140 CPU sec, 2.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283538, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (282.21, 151.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 168, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (100.42, 98.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672834 sequences (100000235 bp) in 1.510 CPU sec, 1.514 real sec...
[M::process] read 672948 sequences (100000164 bp) in 1.470 CPU sec, 1.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283617, 20, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (283.69, 153.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 98, 779)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2329)
[M::mem_pestat] mean and std.dev: (232.33, 337.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3104)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672954 reads in 183.661 CPU sec, 5.798 real sec
sorting block 65, #reads = 1073886 mem = 536878288 peak = 887463936
[M::mem_process_seqs] Processed 672832 reads in 99.978 CPU sec, 3.173 real sec
sorting block 66, #reads = 1077244 mem = 536882132 peak = 921899008
[M::process] read 673174 sequences (100000235 bp) in 2.366 CPU sec, 2.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283069, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (284.27, 154.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673100 sequences (100000228 bp) in 1.530 CPU sec, 1.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284475, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.07, 146.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 208, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (294.70, 309.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1534)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283733, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (279.08, 150.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672834 reads in 280.605 CPU sec, 8.975 real sec
[M::mem_process_seqs] Processed 673174 reads in 192.234 CPU sec, 6.174 real sec
[M::mem_process_seqs] Processed 672948 reads in 199.367 CPU sec, 6.549 real sec
sorting block 67, #reads = 1079413 mem = 536878255 peak = 921899008
[M::process] read 673146 sequences (100000097 bp) in 1.961 CPU sec, 2.172 real sec...
sorting block 68, #reads = 1079515 mem = 536877823 peak = 921899008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283960, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.82, 146.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673142 sequences (100000015 bp) in 1.575 CPU sec, 1.613 real sec...
[M::process] read 673442 sequences (100000184 bp) in 1.470 CPU sec, 1.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284974, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.69, 146.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673100 reads in 182.240 CPU sec, 5.820 real sec
[M::mem_process_seqs] Processed 673146 reads in 100.218 CPU sec, 3.182 real sec
sorting block 69, #reads = 1078762 mem = 536877958 peak = 921899008
[M::process] read 673422 sequences (100000204 bp) in 2.030 CPU sec, 2.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285219, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (274.51, 147.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 29, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224)
[M::mem_pestat] mean and std.dev: (81.56, 125.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1627)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673182 sequences (100000006 bp) in 1.045 CPU sec, 1.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285035, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (269.47, 142.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673142 reads in 173.969 CPU sec, 5.560 real sec
[M::mem_process_seqs] Processed 673442 reads in 95.883 CPU sec, 3.073 real sec
sorting block 70, #reads = 1076883 mem = 536881778 peak = 921899008
[M::process] read 673082 sequences (100000126 bp) in 1.821 CPU sec, 1.885 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284477, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.34, 140.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673068 sequences (100000130 bp) in 1.490 CPU sec, 1.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284307, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.62, 141.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284148, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.89, 142.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673422 reads in 264.345 CPU sec, 8.460 real sec
[M::mem_process_seqs] Processed 673182 reads in 184.020 CPU sec, 5.919 real sec
sorting block 71, #reads = 1078656 mem = 536877793 peak = 921899008
[M::mem_process_seqs] Processed 673082 reads in 190.897 CPU sec, 6.161 real sec
sorting block 72, #reads = 1079357 mem = 536879371 peak = 921899008
[M::process] read 673004 sequences (100000104 bp) in 2.508 CPU sec, 2.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284091, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.46, 141.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 81, 122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208)
[M::mem_pestat] mean and std.dev: (87.31, 30.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 251)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673058 sequences (100000156 bp) in 1.129 CPU sec, 1.130 real sec...
[M::process] read 672960 sequences (100000299 bp) in 1.050 CPU sec, 1.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284108, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.23, 143.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673068 reads in 180.459 CPU sec, 5.769 real sec
[M::mem_process_seqs] Processed 673004 reads in 96.955 CPU sec, 3.090 real sec
sorting block 73, #reads = 1079208 mem = 536881225 peak = 921899008
[M::process] read 672822 sequences (100000057 bp) in 1.744 CPU sec, 1.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284096, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.50, 148.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672874 sequences (100000213 bp) in 1.110 CPU sec, 1.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284191, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.56, 150.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284273, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (280.92, 148.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 36, 63)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 131)
[M::mem_pestat] mean and std.dev: (40.31, 33.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 172)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673058 reads in 247.894 CPU sec, 7.948 real sec
[M::mem_process_seqs] Processed 672822 reads in 175.606 CPU sec, 5.658 real sec
sorting block 74, #reads = 1078292 mem = 536882303 peak = 921899008
[M::mem_process_seqs] Processed 672960 reads in 183.074 CPU sec, 5.918 real sec
sorting block 75, #reads = 1076416 mem = 536879665 peak = 921899008
[M::process] read 672744 sequences (100000218 bp) in 2.201 CPU sec, 2.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284048, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (284.28, 152.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672980 sequences (100000004 bp) in 1.208 CPU sec, 1.226 real sec...
[M::process] read 673116 sequences (100000214 bp) in 1.110 CPU sec, 1.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283112, 24, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (283.78, 151.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 47, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184)
[M::mem_pestat] mean and std.dev: (177.95, 241.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1576)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672874 reads in 185.447 CPU sec, 5.933 real sec
[M::mem_process_seqs] Processed 672744 reads in 103.691 CPU sec, 3.309 real sec
sorting block 76, #reads = 1074544 mem = 536879764 peak = 921899008
[M::process] read 673098 sequences (100000206 bp) in 1.765 CPU sec, 1.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284037, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (275.83, 145.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672952 sequences (100000022 bp) in 1.080 CPU sec, 1.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284095, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (275.55, 145.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283735, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (276.90, 146.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672980 reads in 259.981 CPU sec, 8.346 real sec
sorting block 77, #reads = 1077825 mem = 536880069 peak = 921899008
[M::mem_process_seqs] Processed 673116 reads in 182.016 CPU sec, 5.872 real sec
[M::mem_process_seqs] Processed 673098 reads in 189.059 CPU sec, 6.128 real sec
sorting block 78, #reads = 1078220 mem = 536880967 peak = 921899008
[M::process] read 672906 sequences (100000259 bp) in 1.783 CPU sec, 1.962 real sec...
[M::process] read 238086 sequences (35379163 bp) in 0.723 CPU sec, 0.772 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284767, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (275.69, 145.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284245, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (281.02, 151.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 100506, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (284.80, 156.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672952 reads in 206.489 CPU sec, 6.661 real sec
[M::mem_process_seqs] Processed 672906 reads in 127.789 CPU sec, 4.151 real sec
sorting block 79, #reads = 1079363 mem = 536877045 peak = 921899008
[M::mem_process_seqs] Processed 238086 reads in 130.561 CPU sec, 4.250 real sec
sorting block 80, #reads = 1079609 mem = 536880391 peak = 969953280
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 87051900
overall: 69797769216 mem 15087.331 user 540.677 sys 939.373 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L002\tPU:L002\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.T18-D0003255_R1.fastq.gz 0003.T18-D0003255_R2.fastq.gz
[main] Real time: 939.404 sec; CPU: 15628.011 sec
sorting block 81, #reads = 287386 mem = 142962185 peak = 969953280
uniq header count 1
merging 82 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 3380649555
output reads: 87685752
bam_mem_sort: 82 calls 57.042 user 0.767 sys 57.919 real
bam_write: 82 calls 30.543 user 2.076 sys 33.540 real
execute: 1 calls 18.455 user 1.381 sys 960.399 real
merge_files: 1 calls 18.429 user 1.358 sys 20.825 real
parse_chunk: 1301 calls 57.461 user 15.232 sys 74.012 real
read_chunk: 3501 calls 35.970 user 21.310 sys 451.113 real
sort_block: 1 calls 28.161 user 20.695 sys 439.671 real
write_chunk: 2198 calls 18.070 user 1.128 sys 19.763 real
overall: 2302648320 mem 817.080 user 60.452 sys 960.418 real
ls: cannot access '*.cram': No such file or directory