.command.log - Workdir - DAQ:SENTIEON_BWAMEM (T21-D0003322;L008;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/dc/8af551d8c6f0ebae712405dda84ab6/.command.log
Size: 343.6 KB
Task: DAQ:SENTIEON_BWAMEM (T21-D0003322;L008;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a2/45a6bea2831dc31f2dff518cef3465/T21-D0003322/T21-D0003322_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/dc/8af551d8c6f0ebae712405dda84ab6/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a2/45a6bea2831dc31f2dff518cef3465/T21-D0003322/T21-D0003322_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/dc/8af551d8c6f0ebae712405dda84ab6/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003322.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2340.021 CPU sec, 84.524 real sec
[M::process] read 676008 sequences (100000235 bp) in 1.838 CPU sec, 1.842 real sec...
[M::process] read 675884 sequences (100000019 bp) in 1.960 CPU sec, 1.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286931, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.66, 115.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 17, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1571)
[M::mem_pestat] mean and std.dev: (231.64, 266.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2092)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675738 sequences (100000204 bp) in 1.995 CPU sec, 2.040 real sec...
[M::process] read 675710 sequences (100000045 bp) in 1.980 CPU sec, 2.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286790, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.78, 116.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 20, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1205)
[M::mem_pestat] mean and std.dev: (222.15, 265.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1600)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676008 reads in 244.723 CPU sec, 7.897 real sec
[M::mem_process_seqs] Processed 675884 reads in 136.901 CPU sec, 4.535 real sec
sorting block 0, #reads = 1081605 mem = 536879780 peak = 586747904
[M::process] read 675746 sequences (100000269 bp) in 2.242 CPU sec, 2.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286724, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.51, 117.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 623, 839)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2395)
[M::mem_pestat] mean and std.dev: (509.30, 327.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3173)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675870 sequences (100000140 bp) in 1.825 CPU sec, 1.853 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286524, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.60, 118.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286695, 16, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.66, 118.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 24, 574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1696)
[M::mem_pestat] mean and std.dev: (285.19, 384.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2257)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675738 reads in 358.965 CPU sec, 11.707 real sec
[M::mem_process_seqs] Processed 675710 reads in 249.827 CPU sec, 8.205 real sec
[M::mem_process_seqs] Processed 675746 reads in 260.929 CPU sec, 8.598 real sec
sorting block 1, #reads = 1080536 mem = 536876945 peak = 701456384
[M::process] read 675704 sequences (100000122 bp) in 2.613 CPU sec, 2.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287134, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.57, 119.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1081144 mem = 536877225 peak = 942874624
[M::process] read 675668 sequences (100000073 bp) in 2.577 CPU sec, 2.717 real sec...
[M::process] read 675530 sequences (100000201 bp) in 1.913 CPU sec, 1.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286379, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.09, 119.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675870 reads in 262.247 CPU sec, 8.534 real sec
[M::mem_process_seqs] Processed 675704 reads in 148.861 CPU sec, 4.805 real sec
sorting block 3, #reads = 1081253 mem = 536878933 peak = 942874624
[M::process] read 675446 sequences (100000191 bp) in 2.594 CPU sec, 2.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286516, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.29, 120.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675536 sequences (100000086 bp) in 1.880 CPU sec, 1.889 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287051, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.55, 121.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (103, 518, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1348)
[M::mem_pestat] mean and std.dev: (351.50, 294.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1763)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286389, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.52, 120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (469, 653, 666)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (75, 1060)
[M::mem_pestat] mean and std.dev: (558.25, 186.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675668 reads in 400.266 CPU sec, 13.107 real sec
[M::mem_process_seqs] Processed 675446 reads in 279.603 CPU sec, 9.112 real sec
[M::mem_process_seqs] Processed 675530 reads in 291.389 CPU sec, 9.544 real sec
sorting block 4, #reads = 1080465 mem = 536879052 peak = 942874624
[M::process] read 675312 sequences (100000283 bp) in 2.716 CPU sec, 2.856 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285774, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.30, 121.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 5, #reads = 1081428 mem = 536877930 peak = 942874624
[M::process] read 675310 sequences (100000027 bp) in 2.557 CPU sec, 2.990 real sec...
[M::process] read 675272 sequences (100000288 bp) in 2.018 CPU sec, 2.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286563, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.30, 123.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 3, 41)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 119)
[M::mem_pestat] mean and std.dev: (15.00, 22.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 158)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675536 reads in 264.184 CPU sec, 8.769 real sec
[M::mem_process_seqs] Processed 675312 reads in 149.520 CPU sec, 4.881 real sec
sorting block 6, #reads = 1080640 mem = 536877916 peak = 942874624
[M::process] read 675070 sequences (100000051 bp) in 2.658 CPU sec, 2.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286365, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.43, 123.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675156 sequences (100000245 bp) in 1.934 CPU sec, 1.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286304, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.31, 124.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 25, 42)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 76)
[M::mem_pestat] mean and std.dev: (22.44, 15.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 93)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675310 reads in 253.931 CPU sec, 8.265 real sec
[M::mem_process_seqs] Processed 675272 reads in 142.804 CPU sec, 4.612 real sec
sorting block 7, #reads = 1080684 mem = 536878698 peak = 950210560
[M::process] read 675148 sequences (100000208 bp) in 2.370 CPU sec, 2.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285920, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.35, 124.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675228 sequences (100000017 bp) in 1.943 CPU sec, 1.953 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286108, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.64, 125.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285703, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.88, 126.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675070 reads in 376.246 CPU sec, 12.152 real sec
[M::mem_process_seqs] Processed 675156 reads in 264.350 CPU sec, 8.561 real sec
sorting block 8, #reads = 1080768 mem = 536879100 peak = 950210560
[M::mem_process_seqs] Processed 675148 reads in 275.951 CPU sec, 8.955 real sec
[M::process] read 675014 sequences (100000017 bp) in 2.394 CPU sec, 2.603 real sec...
sorting block 9, #reads = 1080566 mem = 536878999 peak = 989401088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285188, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.44, 127.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675086 sequences (100000164 bp) in 2.595 CPU sec, 2.870 real sec...
[M::process] read 675100 sequences (100000005 bp) in 1.939 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285419, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (254.04, 127.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675228 reads in 267.957 CPU sec, 8.706 real sec
[M::mem_process_seqs] Processed 675014 reads in 151.027 CPU sec, 4.839 real sec
sorting block 10, #reads = 1080964 mem = 536878248 peak = 994209792
[M::process] read 675044 sequences (100000206 bp) in 2.381 CPU sec, 2.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285775, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.10, 126.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674984 sequences (100000288 bp) in 1.979 CPU sec, 1.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286204, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.72, 125.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285948, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.27, 128.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675086 reads in 374.882 CPU sec, 12.058 real sec
sorting block 11, #reads = 1079806 mem = 536881039 peak = 1012228096
[M::mem_process_seqs] Processed 675100 reads in 263.696 CPU sec, 8.611 real sec
[M::mem_process_seqs] Processed 675044 reads in 275.547 CPU sec, 9.032 real sec
sorting block 12, #reads = 1080353 mem = 536878339 peak = 1045311488
[M::process] read 674866 sequences (100000065 bp) in 2.861 CPU sec, 3.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285863, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.98, 129.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 128, 158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328)
[M::mem_pestat] mean and std.dev: (91.22, 56.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 413)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674854 sequences (100000114 bp) in 2.766 CPU sec, 2.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285340, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.79, 129.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 20, 88)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238)
[M::mem_pestat] mean and std.dev: (28.17, 27.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 313)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674934 sequences (100000002 bp) in 2.031 CPU sec, 2.064 real sec...
[M::mem_process_seqs] Processed 674984 reads in 263.359 CPU sec, 8.617 real sec
sorting block 13, #reads = 1080343 mem = 536878701 peak = 1045311488
[M::mem_process_seqs] Processed 674866 reads in 149.436 CPU sec, 4.765 real sec
sorting block 14, #reads = 1080347 mem = 536878879 peak = 1061343232
[M::process] read 674854 sequences (100000042 bp) in 2.690 CPU sec, 2.936 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285196, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.21, 130.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674872 sequences (100000231 bp) in 2.002 CPU sec, 2.009 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285409, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.77, 130.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 33, 178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (72.15, 77.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285973, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.79, 131.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674854 reads in 370.834 CPU sec, 11.919 real sec
[M::mem_process_seqs] Processed 674934 reads in 257.294 CPU sec, 8.263 real sec
[M::mem_process_seqs] Processed 674854 reads in 268.335 CPU sec, 8.639 real sec
sorting block 15, #reads = 1080140 mem = 536879894 peak = 1061343232
[M::process] read 674644 sequences (100000268 bp) in 2.395 CPU sec, 2.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285315, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.83, 132.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1080307 mem = 536877529 peak = 1061343232
[M::process] read 674398 sequences (100000138 bp) in 2.655 CPU sec, 3.087 real sec...
[M::process] read 674648 sequences (100000073 bp) in 2.072 CPU sec, 2.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285138, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.63, 134.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (430, 732, 1494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3622)
[M::mem_pestat] mean and std.dev: (694.64, 553.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4686)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674872 reads in 263.092 CPU sec, 8.564 real sec
[M::mem_process_seqs] Processed 674644 reads in 149.233 CPU sec, 4.786 real sec
sorting block 17, #reads = 1080123 mem = 536879329 peak = 1061343232
[M::process] read 674648 sequences (100000105 bp) in 2.797 CPU sec, 3.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285182, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.37, 137.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674684 sequences (100000236 bp) in 2.037 CPU sec, 2.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285188, 14, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.73, 131.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 15, 15)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 39)
[M::mem_pestat] mean and std.dev: (10.46, 8.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 51)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674398 reads in 258.380 CPU sec, 8.347 real sec
[M::mem_process_seqs] Processed 674648 reads in 146.410 CPU sec, 4.701 real sec
sorting block 18, #reads = 1079726 mem = 536879128 peak = 1061343232
[M::process] read 674670 sequences (100000117 bp) in 2.802 CPU sec, 3.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285595, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.84, 132.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674652 sequences (100000025 bp) in 2.075 CPU sec, 2.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285309, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.16, 132.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285392, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.96, 132.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674648 reads in 377.008 CPU sec, 12.122 real sec
[M::mem_process_seqs] Processed 674684 reads in 264.995 CPU sec, 8.503 real sec
[M::mem_process_seqs] Processed 674670 reads in 276.316 CPU sec, 8.888 real sec
sorting block 19, #reads = 1079867 mem = 536880168 peak = 1061343232
sorting block 20, #reads = 1080191 mem = 536877877 peak = 1103171584
[M::process] read 674628 sequences (100000074 bp) in 2.609 CPU sec, 2.695 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285334, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.84, 132.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 718, 2429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7261)
[M::mem_pestat] mean and std.dev: (1517.22, 1845.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9677)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674518 sequences (100000016 bp) in 2.469 CPU sec, 2.764 real sec...
[M::process] read 674694 sequences (100000268 bp) in 2.098 CPU sec, 2.114 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285487, 15, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.07, 132.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 58, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (109.62, 97.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 582)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674652 reads in 257.834 CPU sec, 8.291 real sec
[M::mem_process_seqs] Processed 674628 reads in 147.314 CPU sec, 4.709 real sec
sorting block 21, #reads = 1080474 mem = 536880263 peak = 1103171584
[M::process] read 674664 sequences (100000254 bp) in 2.822 CPU sec, 3.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285243, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.96, 133.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 17, 17)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 25)
[M::mem_pestat] mean and std.dev: (14.33, 2.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 29)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674608 sequences (100000097 bp) in 2.155 CPU sec, 2.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285652, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.89, 136.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 58, 1121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3247)
[M::mem_pestat] mean and std.dev: (467.70, 533.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4310)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285803, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.09, 134.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 596, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1462)
[M::mem_pestat] mean and std.dev: (502.47, 384.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2039)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674518 reads in 376.547 CPU sec, 12.082 real sec
[M::mem_process_seqs] Processed 674664 reads in 263.864 CPU sec, 8.465 real sec
sorting block 22, #reads = 1080214 mem = 536880812 peak = 1103171584
[M::mem_process_seqs] Processed 674694 reads in 275.330 CPU sec, 8.860 real sec
sorting block 23, #reads = 1080313 mem = 536880591 peak = 1103171584
[M::process] read 674492 sequences (100000040 bp) in 3.028 CPU sec, 3.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285966, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.02, 136.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674514 sequences (100000077 bp) in 2.514 CPU sec, 2.865 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285627, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.75, 136.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674518 sequences (100000071 bp) in 2.105 CPU sec, 2.117 real sec...
[M::mem_process_seqs] Processed 674608 reads in 261.051 CPU sec, 8.491 real sec
[M::mem_process_seqs] Processed 674492 reads in 146.650 CPU sec, 4.736 real sec
sorting block 24, #reads = 1080327 mem = 536880902 peak = 1103171584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285365, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.34, 137.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 53, 121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329)
[M::mem_pestat] mean and std.dev: (44.67, 38.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 433)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674496 sequences (100000031 bp) in 2.966 CPU sec, 3.270 real sec...
[M::process] read 674496 sequences (100000202 bp) in 2.147 CPU sec, 2.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285722, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.81, 136.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674514 reads in 254.195 CPU sec, 8.162 real sec
[M::mem_process_seqs] Processed 674518 reads in 143.703 CPU sec, 4.589 real sec
sorting block 25, #reads = 1080220 mem = 536878826 peak = 1105330176
sorting block 26, #reads = 1079976 mem = 536879445 peak = 1144336384
[M::process] read 674564 sequences (100000051 bp) in 2.530 CPU sec, 2.822 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285584, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.94, 137.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674474 sequences (100000135 bp) in 2.132 CPU sec, 2.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285545, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.61, 139.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286004, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.95, 138.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 50, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (148.33, 155.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1360)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674496 reads in 368.424 CPU sec, 11.866 real sec
[M::mem_process_seqs] Processed 674496 reads in 255.499 CPU sec, 8.195 real sec
[M::mem_process_seqs] Processed 674564 reads in 266.748 CPU sec, 8.585 real sec
sorting block 27, #reads = 1080140 mem = 536879239 peak = 1144336384
[M::process] read 674574 sequences (100000047 bp) in 3.100 CPU sec, 3.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285582, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (264.97, 137.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 121, 6249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18697)
[M::mem_pestat] mean and std.dev: (1956.55, 2703.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 24921)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 28, #reads = 1080164 mem = 536884049 peak = 1144336384
[M::process] read 675870 sequences (100000203 bp) in 2.850 CPU sec, 3.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285509, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.34, 137.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674474 reads in 251.579 CPU sec, 8.125 real sec
[M::mem_process_seqs] Processed 674574 reads in 142.312 CPU sec, 4.551 real sec
[M::process] read 675764 sequences (100000066 bp) in 2.257 CPU sec, 2.375 real sec...
sorting block 29, #reads = 1079471 mem = 536877517 peak = 1144336384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287129, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (238.86, 114.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675920 sequences (100000082 bp) in 2.913 CPU sec, 3.183 real sec...
[M::process] read 675646 sequences (100000008 bp) in 2.100 CPU sec, 2.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286910, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (240.60, 115.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675870 reads in 247.972 CPU sec, 7.964 real sec
[M::mem_process_seqs] Processed 675764 reads in 138.544 CPU sec, 4.416 real sec
sorting block 30, #reads = 1079340 mem = 536880084 peak = 1144336384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286897, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.16, 116.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 98, 749)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2225)
[M::mem_pestat] mean and std.dev: (139.00, 223.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2963)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675754 sequences (100000033 bp) in 2.625 CPU sec, 2.763 real sec...
[M::process] read 675686 sequences (100000013 bp) in 2.191 CPU sec, 2.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287118, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.37, 117.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675920 reads in 239.001 CPU sec, 7.656 real sec
[M::mem_process_seqs] Processed 675646 reads in 137.702 CPU sec, 4.392 real sec
sorting block 31, #reads = 1080880 mem = 536879135 peak = 1144336384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286961, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.65, 117.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675600 sequences (100000123 bp) in 2.803 CPU sec, 3.085 real sec...
[M::process] read 675566 sequences (100000190 bp) in 2.169 CPU sec, 2.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286940, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.46, 118.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 185, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (198.55, 139.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675754 reads in 244.839 CPU sec, 7.848 real sec
[M::mem_process_seqs] Processed 675686 reads in 138.768 CPU sec, 4.409 real sec
sorting block 32, #reads = 1081417 mem = 536878006 peak = 1144336384
sorting block 33, #reads = 1081336 mem = 536877949 peak = 1144336384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286711, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.57, 118.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675550 sequences (100000168 bp) in 2.700 CPU sec, 2.986 real sec...
[M::process] read 675632 sequences (100000099 bp) in 2.135 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286544, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (244.98, 119.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675600 reads in 240.544 CPU sec, 7.771 real sec
[M::mem_process_seqs] Processed 675566 reads in 137.324 CPU sec, 4.431 real sec
sorting block 34, #reads = 1080607 mem = 536879274 peak = 1144336384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286375, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.28, 120.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675434 sequences (100000227 bp) in 3.164 CPU sec, 3.278 real sec...
[M::process] read 675410 sequences (100000113 bp) in 2.100 CPU sec, 2.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286367, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.19, 121.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 345, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1529)
[M::mem_pestat] mean and std.dev: (362.38, 364.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2028)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675550 reads in 246.477 CPU sec, 7.895 real sec
[M::mem_process_seqs] Processed 675632 reads in 140.207 CPU sec, 4.466 real sec
sorting block 35, #reads = 1081016 mem = 536880325 peak = 1144336384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286405, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.13, 122.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675486 sequences (100000116 bp) in 2.391 CPU sec, 2.735 real sec...
[M::process] read 675388 sequences (100000178 bp) in 2.062 CPU sec, 2.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286183, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.24, 124.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 13, 32)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 86)
[M::mem_pestat] mean and std.dev: (19.60, 18.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 113)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675434 reads in 244.562 CPU sec, 7.880 real sec
[M::mem_process_seqs] Processed 675410 reads in 139.493 CPU sec, 4.444 real sec
sorting block 36, #reads = 1081230 mem = 536879963 peak = 1144336384
[M::process] read 675260 sequences (100000221 bp) in 2.396 CPU sec, 2.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286539, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.18, 123.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675222 sequences (100000143 bp) in 2.070 CPU sec, 2.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286268, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.69, 123.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675486 reads in 244.037 CPU sec, 7.874 real sec
[M::mem_process_seqs] Processed 675388 reads in 136.551 CPU sec, 4.384 real sec
sorting block 37, #reads = 1080380 mem = 536879581 peak = 1166684160
sorting block 38, #reads = 1080928 mem = 536879166 peak = 1181827072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285772, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.19, 125.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675312 sequences (100000262 bp) in 2.538 CPU sec, 3.101 real sec...
[M::process] read 675066 sequences (100000059 bp) in 2.128 CPU sec, 2.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286246, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.96, 126.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675260 reads in 251.823 CPU sec, 8.192 real sec
[M::mem_process_seqs] Processed 675222 reads in 142.601 CPU sec, 4.564 real sec
sorting block 39, #reads = 1080690 mem = 536882214 peak = 1181827072
[M::process] read 675042 sequences (100000178 bp) in 2.684 CPU sec, 2.761 real sec...
[M::process] read 675044 sequences (100000150 bp) in 2.040 CPU sec, 2.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286059, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.70, 128.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675066 reads in 129.682 CPU sec, 4.135 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286554, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.76, 126.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 40, #reads = 1080292 mem = 536881341 peak = 1181827072
[M::process] read 675084 sequences (100000285 bp) in 1.970 CPU sec, 1.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285857, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.49, 126.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675312 reads in 374.073 CPU sec, 11.972 real sec
[M::mem_process_seqs] Processed 675042 reads in 268.706 CPU sec, 8.582 real sec
sorting block 41, #reads = 1080941 mem = 536877183 peak = 1181827072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286241, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.26, 127.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674976 sequences (100000104 bp) in 2.509 CPU sec, 3.205 real sec...
[M::process] read 674938 sequences (100000093 bp) in 2.065 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286039, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.33, 127.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675044 reads in 246.079 CPU sec, 7.968 real sec
[M::mem_process_seqs] Processed 675084 reads in 137.541 CPU sec, 4.426 real sec
sorting block 42, #reads = 1080493 mem = 536879411 peak = 1181827072
[M::process] read 675084 sequences (100000261 bp) in 2.609 CPU sec, 2.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286022, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.80, 127.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674862 sequences (100000032 bp) in 2.058 CPU sec, 2.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285322, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.60, 129.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285827, 29, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.35, 130.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 623, 1121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2931)
[M::mem_pestat] mean and std.dev: (552.25, 468.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3836)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674976 reads in 367.166 CPU sec, 11.883 real sec
[M::mem_process_seqs] Processed 674938 reads in 257.656 CPU sec, 8.321 real sec
[M::mem_process_seqs] Processed 675084 reads in 269.288 CPU sec, 8.719 real sec
sorting block 43, #reads = 1080970 mem = 536879843 peak = 1181827072
sorting block 44, #reads = 1080478 mem = 536879403 peak = 1181827072
[M::process] read 674896 sequences (100000290 bp) in 2.945 CPU sec, 3.059 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285571, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (258.99, 132.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674944 sequences (100000129 bp) in 2.624 CPU sec, 2.824 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285110, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.56, 131.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674912 sequences (100000247 bp) in 2.135 CPU sec, 2.149 real sec...
[M::mem_process_seqs] Processed 674862 reads in 257.600 CPU sec, 8.230 real sec
[M::mem_process_seqs] Processed 674896 reads in 143.355 CPU sec, 4.544 real sec
sorting block 45, #reads = 1080219 mem = 536877953 peak = 1181827072
[M::process] read 674934 sequences (100000112 bp) in 2.503 CPU sec, 2.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285930, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.50, 131.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 217, 1966)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5880)
[M::mem_pestat] mean and std.dev: (833.29, 931.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7837)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674702 sequences (100000050 bp) in 2.033 CPU sec, 2.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285831, 25, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (258.92, 132.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 24, 98)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258)
[M::mem_pestat] mean and std.dev: (45.77, 42.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 338)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286090, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.18, 132.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 31, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449)
[M::mem_pestat] mean and std.dev: (142.17, 203.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1931)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674944 reads in 365.491 CPU sec, 11.858 real sec
[M::mem_process_seqs] Processed 674912 reads in 256.028 CPU sec, 8.258 real sec
[M::mem_process_seqs] Processed 674934 reads in 267.154 CPU sec, 8.641 real sec
sorting block 46, #reads = 1079780 mem = 536879982 peak = 1186156544
[M::process] read 674766 sequences (100000176 bp) in 2.622 CPU sec, 2.767 real sec...
sorting block 47, #reads = 1080493 mem = 536881765 peak = 1203576832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286319, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.79, 133.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674638 sequences (100000268 bp) in 2.716 CPU sec, 3.229 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285666, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.82, 132.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 587, 3431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10249)
[M::mem_pestat] mean and std.dev: (1184.50, 1485.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13658)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674702 reads in 253.017 CPU sec, 8.204 real sec
[M::mem_process_seqs] Processed 674766 reads in 144.980 CPU sec, 4.634 real sec
[M::process] read 674792 sequences (100000274 bp) in 2.227 CPU sec, 2.343 real sec...
sorting block 48, #reads = 1080443 mem = 536880632 peak = 1203576832
[M::process] read 674812 sequences (100000192 bp) in 2.380 CPU sec, 2.452 real sec...
[M::process] read 674804 sequences (100000098 bp) in 2.000 CPU sec, 2.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286230, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.61, 134.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 191, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1389)
[M::mem_pestat] mean and std.dev: (273.00, 286.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1830)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674812 reads in 131.951 CPU sec, 4.213 real sec
sorting block 49, #reads = 1080536 mem = 536882180 peak = 1203576832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285444, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.18, 133.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674796 sequences (100000026 bp) in 2.110 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285612, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.03, 132.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674638 reads in 371.939 CPU sec, 11.903 real sec
[M::mem_process_seqs] Processed 674792 reads in 267.389 CPU sec, 8.547 real sec
sorting block 50, #reads = 1079894 mem = 536879900 peak = 1203576832
[M::process] read 674648 sequences (100000082 bp) in 2.706 CPU sec, 3.284 real sec...
[M::process] read 674712 sequences (100000086 bp) in 2.097 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286085, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.62, 135.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674796 reads in 131.157 CPU sec, 4.168 real sec
sorting block 51, #reads = 1080168 mem = 536876921 peak = 1203576832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286159, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.26, 134.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674656 sequences (100000007 bp) in 2.071 CPU sec, 2.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285816, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.04, 136.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674804 reads in 375.760 CPU sec, 12.235 real sec
[M::mem_process_seqs] Processed 674648 reads in 154.805 CPU sec, 5.069 real sec
sorting block 52, #reads = 1080634 mem = 536877329 peak = 1203576832
[M::process] read 674720 sequences (100000048 bp) in 2.500 CPU sec, 2.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285941, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.76, 137.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 88, 1575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4661)
[M::mem_pestat] mean and std.dev: (518.73, 661.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6204)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674616 sequences (100000033 bp) in 1.994 CPU sec, 1.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285930, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.11, 137.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285272, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.33, 136.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 176, 176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486)
[M::mem_pestat] mean and std.dev: (109.56, 107.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674712 reads in 366.041 CPU sec, 11.766 real sec
[M::mem_process_seqs] Processed 674656 reads in 256.992 CPU sec, 8.251 real sec
[M::mem_process_seqs] Processed 674720 reads in 268.698 CPU sec, 8.653 real sec
sorting block 53, #reads = 1080399 mem = 536878229 peak = 1203576832
[M::process] read 674462 sequences (100000091 bp) in 2.542 CPU sec, 2.846 real sec...
sorting block 54, #reads = 1079478 mem = 536878244 peak = 1217482752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285834, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.73, 136.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 265, 730)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2172)
[M::mem_pestat] mean and std.dev: (437.40, 621.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2924)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674602 sequences (100000240 bp) in 2.506 CPU sec, 3.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285750, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.82, 137.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674540 sequences (100000075 bp) in 1.909 CPU sec, 1.918 real sec...
[M::mem_process_seqs] Processed 674616 reads in 248.495 CPU sec, 8.094 real sec
[M::mem_process_seqs] Processed 674462 reads in 140.669 CPU sec, 4.503 real sec
sorting block 55, #reads = 1079841 mem = 536880631 peak = 1217482752
[M::process] read 674496 sequences (100000157 bp) in 2.693 CPU sec, 3.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286477, 22, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.27, 138.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 38, 38)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 78)
[M::mem_pestat] mean and std.dev: (27.78, 11.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 98)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674616 sequences (100000074 bp) in 1.947 CPU sec, 2.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286149, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (267.85, 140.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674602 reads in 243.562 CPU sec, 7.877 real sec
[M::mem_process_seqs] Processed 674540 reads in 136.886 CPU sec, 4.382 real sec
sorting block 56, #reads = 1079993 mem = 536879018 peak = 1217482752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286037, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (267.67, 139.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 189, 190)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (135.50, 85.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 57, #reads = 1080486 mem = 536879619 peak = 1231351808
[M::process] read 674624 sequences (100000060 bp) in 2.444 CPU sec, 2.877 real sec...
[M::process] read 675158 sequences (100000143 bp) in 2.088 CPU sec, 2.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286215, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.48, 138.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674496 reads in 242.280 CPU sec, 7.880 real sec
[M::mem_process_seqs] Processed 674616 reads in 138.520 CPU sec, 4.460 real sec
sorting block 58, #reads = 1079589 mem = 536877801 peak = 1231351808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285540, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.59, 138.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676000 sequences (100000170 bp) in 2.895 CPU sec, 3.093 real sec...
[M::process] read 675958 sequences (100000128 bp) in 2.159 CPU sec, 2.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286039, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.32, 129.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674624 reads in 241.167 CPU sec, 7.768 real sec
[M::mem_process_seqs] Processed 675158 reads in 136.194 CPU sec, 4.346 real sec
sorting block 59, #reads = 1079582 mem = 536882788 peak = 1231351808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286472, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (240.23, 115.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 98, 733)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2185)
[M::mem_pestat] mean and std.dev: (284.00, 362.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2911)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675836 sequences (100000112 bp) in 2.486 CPU sec, 2.817 real sec...
[M::process] read 675846 sequences (100000240 bp) in 2.094 CPU sec, 2.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286341, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.25, 116.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 203, 1952)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5734)
[M::mem_pestat] mean and std.dev: (499.83, 714.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7625)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676000 reads in 241.095 CPU sec, 7.809 real sec
[M::mem_process_seqs] Processed 675958 reads in 137.075 CPU sec, 4.417 real sec
sorting block 60, #reads = 1080322 mem = 536879094 peak = 1231351808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286451, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.67, 116.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675596 sequences (100000232 bp) in 2.801 CPU sec, 3.286 real sec...
[M::process] read 675670 sequences (100000091 bp) in 1.970 CPU sec, 2.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286268, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.59, 117.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675836 reads in 244.504 CPU sec, 7.948 real sec
[M::mem_process_seqs] Processed 675846 reads in 138.838 CPU sec, 4.472 real sec
sorting block 61, #reads = 1080605 mem = 536877704 peak = 1231351808
sorting block 62, #reads = 1080865 mem = 536882236 peak = 1231351808
[M::process] read 675706 sequences (100000162 bp) in 2.371 CPU sec, 2.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286122, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.36, 119.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675560 sequences (100000194 bp) in 2.088 CPU sec, 2.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286739, 17, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.94, 119.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 2115, 2115)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6301)
[M::mem_pestat] mean and std.dev: (1228.12, 967.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8394)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675596 reads in 243.177 CPU sec, 7.842 real sec
[M::mem_process_seqs] Processed 675670 reads in 138.354 CPU sec, 4.437 real sec
sorting block 63, #reads = 1080673 mem = 536877545 peak = 1231351808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286627, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.39, 119.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 59, 101)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285)
[M::mem_pestat] mean and std.dev: (59.92, 47.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 377)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675436 sequences (100000117 bp) in 2.617 CPU sec, 2.892 real sec...
[M::process] read 675454 sequences (100000008 bp) in 2.079 CPU sec, 2.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285915, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.56, 119.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675706 reads in 245.635 CPU sec, 7.932 real sec
[M::mem_process_seqs] Processed 675560 reads in 139.956 CPU sec, 4.504 real sec
sorting block 64, #reads = 1080825 mem = 536879419 peak = 1231351808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286756, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (245.32, 119.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675462 sequences (100000179 bp) in 2.613 CPU sec, 3.419 real sec...
[M::process] read 675370 sequences (100000097 bp) in 2.146 CPU sec, 2.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285799, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (245.97, 120.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675436 reads in 247.793 CPU sec, 8.051 real sec
[M::mem_process_seqs] Processed 675454 reads in 139.196 CPU sec, 4.471 real sec
sorting block 65, #reads = 1080779 mem = 536878217 peak = 1231351808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286589, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.77, 121.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 66, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 169)
[M::mem_pestat] mean and std.dev: (44.79, 24.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 219)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675414 sequences (100000236 bp) in 2.948 CPU sec, 3.290 real sec...
[M::process] read 675438 sequences (100000035 bp) in 2.168 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286092, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.24, 121.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 175, 175)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (175, 175)
[M::mem_pestat] mean and std.dev: (175.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (175, 175)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675462 reads in 248.188 CPU sec, 8.017 real sec
[M::mem_process_seqs] Processed 675370 reads in 141.386 CPU sec, 4.521 real sec
sorting block 66, #reads = 1080956 mem = 536877854 peak = 1231351808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286101, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.54, 122.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1080890 mem = 536878889 peak = 1231351808
[M::process] read 675186 sequences (100000086 bp) in 2.480 CPU sec, 3.312 real sec...
[M::process] read 675222 sequences (100000069 bp) in 2.100 CPU sec, 2.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285771, 24, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.57, 123.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 76, 2370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7096)
[M::mem_pestat] mean and std.dev: (679.25, 989.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9459)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675414 reads in 251.493 CPU sec, 8.254 real sec
[M::mem_process_seqs] Processed 675438 reads in 142.458 CPU sec, 4.567 real sec
sorting block 68, #reads = 1080930 mem = 536880779 peak = 1231351808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286194, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.67, 124.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675092 sequences (100000293 bp) in 2.789 CPU sec, 3.521 real sec...
[M::process] read 675004 sequences (100000003 bp) in 2.115 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286024, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.71, 125.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 2121, 2689)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8049)
[M::mem_pestat] mean and std.dev: (1406.87, 1302.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10729)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675186 reads in 252.221 CPU sec, 8.241 real sec
[M::mem_process_seqs] Processed 675222 reads in 143.224 CPU sec, 4.606 real sec
sorting block 69, #reads = 1080607 mem = 536878652 peak = 1231351808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285635, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.07, 126.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 1708, 1708)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4860)
[M::mem_pestat] mean and std.dev: (1022.44, 746.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6436)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674982 sequences (100000022 bp) in 2.498 CPU sec, 2.819 real sec...
[M::process] read 674966 sequences (100000003 bp) in 2.275 CPU sec, 2.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286300, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.52, 127.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 26, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (107.92, 159.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675092 reads in 251.936 CPU sec, 8.100 real sec
[M::mem_process_seqs] Processed 675004 reads in 140.560 CPU sec, 4.479 real sec
sorting block 70, #reads = 1080705 mem = 536877785 peak = 1237315584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285924, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.38, 127.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675080 sequences (100000246 bp) in 2.755 CPU sec, 3.756 real sec...
[M::process] read 674936 sequences (100000208 bp) in 2.138 CPU sec, 2.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285535, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (255.08, 128.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674982 reads in 252.782 CPU sec, 8.195 real sec
sorting block 71, #reads = 1080827 mem = 536878564 peak = 1246216192
[M::mem_process_seqs] Processed 674966 reads in 142.017 CPU sec, 4.524 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285999, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.46, 126.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 63, 76)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 192)
[M::mem_pestat] mean and std.dev: (41.93, 28.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 250)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 72, #reads = 1080368 mem = 536879329 peak = 1257009152
[M::process] read 675030 sequences (100000167 bp) in 2.486 CPU sec, 3.217 real sec...
[M::process] read 674830 sequences (100000057 bp) in 1.957 CPU sec, 2.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286125, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.69, 128.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 1029, 1029)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2657)
[M::mem_pestat] mean and std.dev: (719.70, 424.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3471)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675080 reads in 252.920 CPU sec, 8.245 real sec
[M::mem_process_seqs] Processed 674936 reads in 143.589 CPU sec, 4.600 real sec
sorting block 73, #reads = 1080569 mem = 536880016 peak = 1257009152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286090, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.15, 128.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674880 sequences (100000100 bp) in 2.720 CPU sec, 3.172 real sec...
[M::process] read 674922 sequences (100000283 bp) in 1.894 CPU sec, 1.933 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285637, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.79, 131.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 26, 100)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284)
[M::mem_pestat] mean and std.dev: (54.14, 69.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 376)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675030 reads in 249.393 CPU sec, 8.049 real sec
[M::mem_process_seqs] Processed 674830 reads in 142.670 CPU sec, 4.555 real sec
sorting block 74, #reads = 1080295 mem = 536877266 peak = 1257009152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285578, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.30, 132.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 184, 626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1808)
[M::mem_pestat] mean and std.dev: (279.38, 325.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2399)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674810 sequences (100000083 bp) in 2.459 CPU sec, 3.246 real sec...
[M::process] read 674780 sequences (100000058 bp) in 2.147 CPU sec, 2.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285792, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.07, 132.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674880 reads in 256.338 CPU sec, 8.324 real sec
[M::mem_process_seqs] Processed 674922 reads in 144.136 CPU sec, 4.600 real sec
sorting block 75, #reads = 1080489 mem = 536879497 peak = 1257009152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285362, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.40, 132.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674748 sequences (100000030 bp) in 2.761 CPU sec, 3.562 real sec...
[M::process] read 674830 sequences (100000132 bp) in 2.081 CPU sec, 2.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285276, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.01, 132.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674810 reads in 258.431 CPU sec, 8.425 real sec
sorting block 76, #reads = 1080051 mem = 536879245 peak = 1257009152
[M::mem_process_seqs] Processed 674780 reads in 146.211 CPU sec, 4.670 real sec
sorting block 77, #reads = 1080030 mem = 536879635 peak = 1257009152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285394, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.68, 133.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 178, 1401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4087)
[M::mem_pestat] mean and std.dev: (706.67, 736.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5430)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674798 sequences (100000163 bp) in 2.553 CPU sec, 3.433 real sec...
[M::process] read 674852 sequences (100000248 bp) in 2.079 CPU sec, 2.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285473, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.45, 133.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674748 reads in 258.042 CPU sec, 8.365 real sec
[M::mem_process_seqs] Processed 674830 reads in 143.473 CPU sec, 4.577 real sec
sorting block 78, #reads = 1080189 mem = 536883269 peak = 1257009152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284992, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.81, 133.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674764 sequences (100000272 bp) in 2.738 CPU sec, 3.256 real sec...
[M::process] read 674658 sequences (100000097 bp) in 2.094 CPU sec, 2.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285851, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.07, 133.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674798 reads in 255.783 CPU sec, 8.268 real sec
[M::mem_process_seqs] Processed 674852 reads in 141.721 CPU sec, 4.526 real sec
sorting block 79, #reads = 1080002 mem = 536878526 peak = 1257009152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285341, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.14, 133.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674560 sequences (100000192 bp) in 2.531 CPU sec, 3.608 real sec...
[M::process] read 674616 sequences (100000046 bp) in 2.137 CPU sec, 2.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285490, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.75, 133.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 441, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1523)
[M::mem_pestat] mean and std.dev: (292.90, 243.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2007)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674764 reads in 255.776 CPU sec, 8.315 real sec
[M::mem_process_seqs] Processed 674658 reads in 144.772 CPU sec, 4.629 real sec
sorting block 80, #reads = 1080570 mem = 536880003 peak = 1257009152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285170, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.53, 132.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 81, #reads = 1080723 mem = 536877970 peak = 1257009152
[M::process] read 674676 sequences (100000298 bp) in 2.719 CPU sec, 3.459 real sec...
[M::process] read 674718 sequences (100000166 bp) in 2.049 CPU sec, 2.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285826, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.71, 134.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 13, 44)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 126)
[M::mem_pestat] mean and std.dev: (26.27, 28.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 167)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674560 reads in 252.744 CPU sec, 8.156 real sec
[M::mem_process_seqs] Processed 674616 reads in 143.067 CPU sec, 4.551 real sec
sorting block 82, #reads = 1079694 mem = 536879836 peak = 1257009152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285828, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.70, 134.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674678 sequences (100000281 bp) in 2.498 CPU sec, 2.975 real sec...
[M::process] read 674648 sequences (100000273 bp) in 2.105 CPU sec, 2.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285544, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.83, 135.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 18, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602)
[M::mem_pestat] mean and std.dev: (205.00, 256.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2130)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674676 reads in 253.366 CPU sec, 8.179 real sec
[M::mem_process_seqs] Processed 674718 reads in 142.362 CPU sec, 4.547 real sec
sorting block 83, #reads = 1080402 mem = 536880119 peak = 1257009152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285667, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.08, 136.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 32, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (128.08, 150.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1365)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674618 sequences (100000133 bp) in 2.782 CPU sec, 3.450 real sec...
[M::process] read 674570 sequences (100000173 bp) in 2.181 CPU sec, 2.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285415, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.90, 136.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674678 reads in 253.458 CPU sec, 8.223 real sec
[M::mem_process_seqs] Processed 674648 reads in 144.222 CPU sec, 4.636 real sec
sorting block 84, #reads = 1080740 mem = 536878144 peak = 1257009152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285804, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.10, 137.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674566 sequences (100000203 bp) in 2.560 CPU sec, 3.350 real sec...
[M::process] read 674502 sequences (100000168 bp) in 2.285 CPU sec, 2.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285714, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.99, 137.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 25, 1401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4197)
[M::mem_pestat] mean and std.dev: (552.57, 784.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5595)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674618 reads in 249.497 CPU sec, 8.068 real sec
[M::mem_process_seqs] Processed 674570 reads in 140.617 CPU sec, 4.495 real sec
sorting block 85, #reads = 1080151 mem = 536879019 peak = 1257009152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285956, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.99, 138.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674534 sequences (100000050 bp) in 2.710 CPU sec, 3.036 real sec...
sorting block 86, #reads = 1080151 mem = 536879783 peak = 1272848384
[M::process] read 674578 sequences (100000062 bp) in 2.057 CPU sec, 2.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285368, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.08, 139.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (109, 2480, 2480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7222)
[M::mem_pestat] mean and std.dev: (1604.64, 1158.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9593)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674566 reads in 248.583 CPU sec, 8.118 real sec
[M::mem_process_seqs] Processed 674502 reads in 138.561 CPU sec, 4.496 real sec
sorting block 87, #reads = 1079947 mem = 536878396 peak = 1272848384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285268, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.91, 139.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674802 sequences (100000239 bp) in 2.848 CPU sec, 3.173 real sec...
[M::process] read 675458 sequences (100000244 bp) in 2.072 CPU sec, 2.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286017, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.69, 137.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674534 reads in 250.380 CPU sec, 8.127 real sec
[M::mem_process_seqs] Processed 674578 reads in 140.983 CPU sec, 4.570 real sec
sorting block 88, #reads = 1080510 mem = 536878667 peak = 1272848384
[M::process] read 675912 sequences (100000274 bp) in 2.877 CPU sec, 3.079 real sec...
[M::process] read 675830 sequences (100000132 bp) in 2.266 CPU sec, 2.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287050, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.56, 122.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675458 reads in 126.870 CPU sec, 4.087 real sec
sorting block 89, #reads = 1080308 mem = 536877952 peak = 1272848384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286272, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.64, 138.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 203, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (176.81, 159.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675720 sequences (100000180 bp) in 2.080 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286855, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (238.46, 113.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674802 reads in 363.266 CPU sec, 11.729 real sec
[M::mem_process_seqs] Processed 675912 reads in 149.806 CPU sec, 4.824 real sec
sorting block 90, #reads = 1080772 mem = 536877960 peak = 1272848384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286640, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (240.38, 115.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675636 sequences (100000023 bp) in 2.753 CPU sec, 3.175 real sec...
[M::process] read 675594 sequences (100000014 bp) in 2.160 CPU sec, 2.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286234, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.78, 116.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 256, 752)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1814)
[M::mem_pestat] mean and std.dev: (452.91, 400.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2345)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675830 reads in 246.189 CPU sec, 7.942 real sec
[M::mem_process_seqs] Processed 675720 reads in 141.285 CPU sec, 4.502 real sec
sorting block 91, #reads = 1081342 mem = 536877243 peak = 1272848384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286517, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.91, 116.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675706 sequences (100000284 bp) in 2.672 CPU sec, 3.584 real sec...
[M::process] read 675528 sequences (100000072 bp) in 2.130 CPU sec, 2.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286306, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.98, 117.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675636 reads in 249.727 CPU sec, 8.138 real sec
[M::mem_process_seqs] Processed 675594 reads in 140.634 CPU sec, 4.496 real sec
sorting block 92, #reads = 1080814 mem = 536879076 peak = 1272848384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286455, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.75, 117.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 10, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1583)
[M::mem_pestat] mean and std.dev: (154.21, 227.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2108)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675534 sequences (100000131 bp) in 2.550 CPU sec, 3.037 real sec...
[M::process] read 675442 sequences (100000276 bp) in 2.148 CPU sec, 2.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286258, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (244.84, 119.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 88, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1769)
[M::mem_pestat] mean and std.dev: (345.17, 567.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2616)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675706 reads in 250.401 CPU sec, 8.125 real sec
sorting block 93, #reads = 1080925 mem = 536879652 peak = 1272848384
[M::mem_process_seqs] Processed 675528 reads in 139.419 CPU sec, 4.428 real sec
sorting block 94, #reads = 1080796 mem = 536878596 peak = 1273491456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286552, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (244.22, 118.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675594 sequences (100000168 bp) in 2.720 CPU sec, 3.583 real sec...
[M::process] read 675398 sequences (100000073 bp) in 2.169 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285753, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.34, 118.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675534 reads in 251.723 CPU sec, 8.143 real sec
[M::mem_process_seqs] Processed 675442 reads in 143.090 CPU sec, 4.568 real sec
sorting block 95, #reads = 1080862 mem = 536884068 peak = 1273491456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285799, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (245.75, 119.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675442 sequences (100000084 bp) in 2.500 CPU sec, 2.994 real sec...
[M::process] read 675406 sequences (100000015 bp) in 2.150 CPU sec, 2.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286417, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (246.23, 120.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 217, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2016)
[M::mem_pestat] mean and std.dev: (139.88, 221.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2687)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675594 reads in 249.532 CPU sec, 8.072 real sec
[M::mem_process_seqs] Processed 675398 reads in 139.401 CPU sec, 4.463 real sec
sorting block 96, #reads = 1080757 mem = 536882008 peak = 1273491456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286340, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (246.00, 119.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675354 sequences (100000063 bp) in 2.719 CPU sec, 3.576 real sec...
[M::process] read 675206 sequences (100000255 bp) in 2.273 CPU sec, 2.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285341, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.51, 120.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675442 reads in 254.677 CPU sec, 8.233 real sec
[M::mem_process_seqs] Processed 675406 reads in 142.831 CPU sec, 4.550 real sec
sorting block 97, #reads = 1080683 mem = 536878859 peak = 1273491456
sorting block 98, #reads = 1080476 mem = 536878497 peak = 1273491456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285312, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.77, 121.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675140 sequences (100000032 bp) in 2.503 CPU sec, 3.145 real sec...
[M::process] read 675018 sequences (100000217 bp) in 2.144 CPU sec, 2.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286383, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.63, 123.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 112, 112)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (112, 112)
[M::mem_pestat] mean and std.dev: (112.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (112, 112)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675354 reads in 254.800 CPU sec, 8.226 real sec
[M::mem_process_seqs] Processed 675206 reads in 141.773 CPU sec, 4.510 real sec
sorting block 99, #reads = 1080121 mem = 536880062 peak = 1273491456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285594, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (250.38, 123.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675068 sequences (100000199 bp) in 2.742 CPU sec, 3.346 real sec...
[M::process] read 675138 sequences (100000144 bp) in 2.132 CPU sec, 2.132 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285250, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.61, 123.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675140 reads in 263.846 CPU sec, 8.542 real sec
[M::mem_process_seqs] Processed 675018 reads in 149.304 CPU sec, 4.770 real sec
sorting block 100, #reads = 1080441 mem = 536880791 peak = 1273491456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285259, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.98, 125.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 27, 128)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368)
[M::mem_pestat] mean and std.dev: (27.90, 34.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674988 sequences (100000047 bp) in 2.473 CPU sec, 3.438 real sec...
[M::process] read 674932 sequences (100000115 bp) in 2.125 CPU sec, 2.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285691, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (253.02, 125.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 14, 22)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 50)
[M::mem_pestat] mean and std.dev: (10.67, 5.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 64)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675068 reads in 260.791 CPU sec, 8.418 real sec
[M::mem_process_seqs] Processed 675138 reads in 145.959 CPU sec, 4.640 real sec
sorting block 101, #reads = 1080293 mem = 536879459 peak = 1273491456
[M::process] read 674872 sequences (100000035 bp) in 2.843 CPU sec, 3.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285039, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.94, 125.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 19, 174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498)
[M::mem_pestat] mean and std.dev: (78.71, 105.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 660)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674934 sequences (100000232 bp) in 2.166 CPU sec, 2.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285896, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.48, 127.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285671, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (254.25, 126.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (97, 200, 1022)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2872)
[M::mem_pestat] mean and std.dev: (592.94, 610.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3797)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674988 reads in 380.382 CPU sec, 12.223 real sec
[M::mem_process_seqs] Processed 674932 reads in 264.589 CPU sec, 8.476 real sec
sorting block 102, #reads = 1079760 mem = 536880149 peak = 1273491456
[M::mem_process_seqs] Processed 674872 reads in 275.856 CPU sec, 8.858 real sec
sorting block 103, #reads = 1080028 mem = 536878590 peak = 1273491456
[M::process] read 674970 sequences (100000020 bp) in 2.609 CPU sec, 2.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285218, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.62, 125.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674874 sequences (100000194 bp) in 2.666 CPU sec, 2.984 real sec...
[M::process] read 674864 sequences (100000091 bp) in 2.144 CPU sec, 2.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285135, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.69, 129.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674934 reads in 265.386 CPU sec, 8.582 real sec
sorting block 104, #reads = 1080575 mem = 536876138 peak = 1273491456
[M::process] read 674676 sequences (100000122 bp) in 2.100 CPU sec, 2.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285292, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.89, 129.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (88, 393, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (242.27, 174.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674874 reads in 143.211 CPU sec, 4.720 real sec
[M::mem_process_seqs] Processed 674970 reads in 337.232 CPU sec, 10.960 real sec
sorting block 105, #reads = 1080282 mem = 536878979 peak = 1273491456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285508, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.21, 129.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674750 sequences (100000023 bp) in 2.710 CPU sec, 3.480 real sec...
[M::process] read 674808 sequences (100000278 bp) in 2.053 CPU sec, 2.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285594, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.40, 131.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 3, 88)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260)
[M::mem_pestat] mean and std.dev: (24.00, 33.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 346)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674864 reads in 276.023 CPU sec, 8.896 real sec
[M::mem_process_seqs] Processed 674676 reads in 147.677 CPU sec, 4.730 real sec
sorting block 106, #reads = 1080714 mem = 536877474 peak = 1273491456
[M::process] read 674798 sequences (100000270 bp) in 2.559 CPU sec, 2.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285197, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.94, 132.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674774 sequences (100000159 bp) in 2.093 CPU sec, 2.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284439, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.71, 130.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284928, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.24, 131.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674750 reads in 383.869 CPU sec, 12.370 real sec
[M::mem_process_seqs] Processed 674808 reads in 269.249 CPU sec, 8.646 real sec
sorting block 107, #reads = 1080127 mem = 536878901 peak = 1273491456
[M::mem_process_seqs] Processed 674798 reads in 280.827 CPU sec, 9.038 real sec
sorting block 108, #reads = 1080113 mem = 536879730 peak = 1273491456
[M::process] read 674762 sequences (100000004 bp) in 3.103 CPU sec, 3.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285531, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.35, 131.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 21, 78)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218)
[M::mem_pestat] mean and std.dev: (34.36, 33.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 288)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674780 sequences (100000140 bp) in 2.874 CPU sec, 3.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285283, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.28, 133.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674660 sequences (100000146 bp) in 2.147 CPU sec, 2.152 real sec...
[M::mem_process_seqs] Processed 674774 reads in 269.264 CPU sec, 8.658 real sec
[M::mem_process_seqs] Processed 674762 reads in 153.618 CPU sec, 4.903 real sec
sorting block 109, #reads = 1080318 mem = 536880536 peak = 1273491456
[M::process] read 674660 sequences (100000211 bp) in 2.501 CPU sec, 2.800 real sec...
sorting block 110, #reads = 1080101 mem = 536879919 peak = 1284612096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284829, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.92, 131.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (65, 390, 1764)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5162)
[M::mem_pestat] mean and std.dev: (819.45, 873.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6861)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674650 sequences (100000189 bp) in 2.203 CPU sec, 2.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284988, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.95, 133.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285423, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.63, 131.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674780 reads in 385.488 CPU sec, 12.418 real sec
[M::mem_process_seqs] Processed 674660 reads in 269.547 CPU sec, 8.654 real sec
[M::mem_process_seqs] Processed 674660 reads in 280.899 CPU sec, 9.054 real sec
sorting block 111, #reads = 1079956 mem = 536878943 peak = 1284612096
[M::process] read 674436 sequences (100000187 bp) in 2.996 CPU sec, 3.251 real sec...
[M::process] read 674642 sequences (100000287 bp) in 2.504 CPU sec, 2.758 real sec...
[M::process] read 674554 sequences (100000095 bp) in 2.066 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285014, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.82, 136.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 8, 1798)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5388)
[M::mem_pestat] mean and std.dev: (557.20, 813.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7183)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674436 reads in 142.782 CPU sec, 4.520 real sec
sorting block 112, #reads = 1079431 mem = 536880173 peak = 1284612096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285124, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.48, 132.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285248, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.27, 134.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674650 reads in 402.722 CPU sec, 13.006 real sec
[M::process] read 674544 sequences (100000172 bp) in 2.231 CPU sec, 2.398 real sec...
[M::mem_process_seqs] Processed 674642 reads in 160.189 CPU sec, 5.201 real sec
sorting block 113, #reads = 1080235 mem = 536880944 peak = 1284612096
[M::process] read 674448 sequences (100000170 bp) in 2.744 CPU sec, 2.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285264, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.15, 134.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674704 sequences (100000238 bp) in 2.083 CPU sec, 2.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284935, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.48, 135.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 72, 970)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2828)
[M::mem_pestat] mean and std.dev: (303.45, 409.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3757)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285100, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.22, 135.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674554 reads in 378.071 CPU sec, 12.146 real sec
[M::mem_process_seqs] Processed 674544 reads in 266.253 CPU sec, 8.547 real sec
sorting block 114, #reads = 1080045 mem = 536878236 peak = 1284612096
[M::mem_process_seqs] Processed 674448 reads in 277.839 CPU sec, 8.941 real sec
sorting block 115, #reads = 1080280 mem = 536877580 peak = 1284612096
[M::process] read 674402 sequences (100000163 bp) in 3.128 CPU sec, 3.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285355, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.37, 136.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674552 sequences (100000163 bp) in 2.697 CPU sec, 3.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285367, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.49, 136.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 175, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (178.50, 176.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 893)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674434 sequences (100000089 bp) in 2.088 CPU sec, 2.096 real sec...
[M::mem_process_seqs] Processed 674704 reads in 264.138 CPU sec, 8.554 real sec
[M::mem_process_seqs] Processed 674402 reads in 148.691 CPU sec, 4.781 real sec
sorting block 116, #reads = 1079944 mem = 536879031 peak = 1284612096
[M::process] read 674522 sequences (100000032 bp) in 2.667 CPU sec, 3.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285106, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.76, 138.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674394 sequences (100000274 bp) in 2.052 CPU sec, 2.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285011, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.24, 138.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 361, 1121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3151)
[M::mem_pestat] mean and std.dev: (566.33, 625.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4166)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285727, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.53, 139.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (552, 1764, 1764)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4188)
[M::mem_pestat] mean and std.dev: (1176.30, 738.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5400)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674552 reads in 374.189 CPU sec, 12.046 real sec
sorting block 117, #reads = 1080241 mem = 536878434 peak = 1284612096
[M::mem_process_seqs] Processed 674434 reads in 261.396 CPU sec, 8.389 real sec
[M::mem_process_seqs] Processed 674522 reads in 272.782 CPU sec, 8.781 real sec
sorting block 118, #reads = 1079366 mem = 536877980 peak = 1287995392
[M::process] read 674760 sequences (100000231 bp) in 2.491 CPU sec, 2.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285618, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.65, 139.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676176 sequences (100000033 bp) in 2.685 CPU sec, 3.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286095, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.39, 135.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675966 sequences (100000066 bp) in 2.153 CPU sec, 2.158 real sec...
[M::mem_process_seqs] Processed 674394 reads in 253.245 CPU sec, 8.184 real sec
sorting block 119, #reads = 1079415 mem = 536878872 peak = 1287995392
[M::mem_process_seqs] Processed 674760 reads in 142.164 CPU sec, 4.510 real sec
sorting block 120, #reads = 1079659 mem = 536876858 peak = 1287995392
[M::process] read 675928 sequences (100000288 bp) in 2.595 CPU sec, 3.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286763, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (236.86, 114.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675966 sequences (100000212 bp) in 2.064 CPU sec, 2.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286425, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (238.21, 115.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676176 reads in 250.736 CPU sec, 8.130 real sec
[M::process] read 675736 sequences (100000261 bp) in 2.400 CPU sec, 2.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286356, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.04, 115.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675928 reads in 133.152 CPU sec, 4.264 real sec
sorting block 121, #reads = 1081230 mem = 536880119 peak = 1287995392
[M::mem_process_seqs] Processed 675966 reads in 355.276 CPU sec, 11.484 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286587, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.15, 115.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 122, #reads = 1080429 mem = 536880155 peak = 1287995392
[M::process] read 675922 sequences (100000237 bp) in 2.737 CPU sec, 2.930 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286534, 22, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (239.90, 115.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 196, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1950)
[M::mem_pestat] mean and std.dev: (289.95, 306.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2588)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675748 sequences (100000083 bp) in 2.181 CPU sec, 2.195 real sec...
[M::mem_process_seqs] Processed 675966 reads in 260.979 CPU sec, 8.388 real sec
[M::mem_process_seqs] Processed 675736 reads in 142.183 CPU sec, 4.510 real sec
sorting block 123, #reads = 1081186 mem = 536879033 peak = 1287995392
[M::process] read 675604 sequences (100000032 bp) in 2.598 CPU sec, 2.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287082, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (238.10, 115.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 22, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (42.25, 66.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675670 sequences (100000133 bp) in 2.072 CPU sec, 2.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286528, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (241.86, 117.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285754, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.41, 117.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 14, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596)
[M::mem_pestat] mean and std.dev: (78.33, 116.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675922 reads in 371.323 CPU sec, 11.926 real sec
[M::mem_process_seqs] Processed 675748 reads in 261.325 CPU sec, 8.387 real sec
[M::mem_process_seqs] Processed 675604 reads in 272.569 CPU sec, 8.769 real sec
sorting block 124, #reads = 1081441 mem = 536879714 peak = 1287995392
[M::process] read 675404 sequences (100000027 bp) in 2.488 CPU sec, 2.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285976, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.90, 120.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 125, #reads = 1080679 mem = 536879616 peak = 1287995392
[M::process] read 675424 sequences (100000094 bp) in 2.695 CPU sec, 3.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286207, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.47, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675670 reads in 258.066 CPU sec, 8.426 real sec
[M::process] read 675372 sequences (100000267 bp) in 2.074 CPU sec, 2.301 real sec...
[M::mem_process_seqs] Processed 675404 reads in 145.543 CPU sec, 4.680 real sec
sorting block 126, #reads = 1080546 mem = 536878899 peak = 1287995392
[M::process] read 675416 sequences (100000101 bp) in 2.616 CPU sec, 2.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285960, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (245.54, 120.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675468 sequences (100000026 bp) in 2.040 CPU sec, 2.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286026, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (246.63, 121.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286471, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.08, 121.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 42, 106)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290)
[M::mem_pestat] mean and std.dev: (40.73, 40.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 382)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675424 reads in 365.643 CPU sec, 11.758 real sec
[M::mem_process_seqs] Processed 675372 reads in 254.432 CPU sec, 8.176 real sec
[M::mem_process_seqs] Processed 675416 reads in 265.796 CPU sec, 8.568 real sec
sorting block 127, #reads = 1080966 mem = 536879340 peak = 1287995392
[M::process] read 675390 sequences (100000113 bp) in 2.840 CPU sec, 3.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286191, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (246.98, 121.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 128, #reads = 1081061 mem = 536878502 peak = 1287995392
[M::process] read 675334 sequences (100000092 bp) in 3.043 CPU sec, 3.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285837, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.96, 123.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 73, 88)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230)
[M::mem_pestat] mean and std.dev: (44.50, 33.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 301)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675468 reads in 258.910 CPU sec, 8.376 real sec
[M::mem_process_seqs] Processed 675390 reads in 148.459 CPU sec, 4.750 real sec
[M::process] read 675254 sequences (100000270 bp) in 2.202 CPU sec, 2.334 real sec...
sorting block 129, #reads = 1080901 mem = 536876950 peak = 1287995392
[M::process] read 675174 sequences (100000171 bp) in 2.415 CPU sec, 2.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285778, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.44, 123.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675108 sequences (100000298 bp) in 2.142 CPU sec, 2.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285367, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.36, 124.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675334 reads in 261.786 CPU sec, 8.473 real sec
[M::mem_process_seqs] Processed 675254 reads in 146.607 CPU sec, 4.674 real sec
sorting block 130, #reads = 1080413 mem = 536877071 peak = 1287995392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285701, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.56, 124.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675284 sequences (100000107 bp) in 2.702 CPU sec, 3.117 real sec...
[M::process] read 675180 sequences (100000164 bp) in 2.125 CPU sec, 2.130 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286003, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.95, 125.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675174 reads in 260.415 CPU sec, 8.395 real sec
[M::mem_process_seqs] Processed 675108 reads in 144.924 CPU sec, 4.618 real sec
sorting block 131, #reads = 1080723 mem = 536878424 peak = 1287995392
sorting block 132, #reads = 1080680 mem = 536878056 peak = 1287995392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286378, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.70, 124.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675164 sequences (100000275 bp) in 2.400 CPU sec, 2.933 real sec...
[M::process] read 675102 sequences (100000176 bp) in 2.095 CPU sec, 2.308 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285654, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.05, 123.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 58, 689)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1951)
[M::mem_pestat] mean and std.dev: (238.60, 299.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2582)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675284 reads in 251.432 CPU sec, 8.144 real sec
[M::mem_process_seqs] Processed 675180 reads in 144.166 CPU sec, 4.611 real sec
sorting block 133, #reads = 1080764 mem = 536880475 peak = 1287995392
[M::process] read 675074 sequences (100000293 bp) in 2.551 CPU sec, 2.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285359, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.67, 124.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675138 sequences (100000169 bp) in 2.114 CPU sec, 2.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285796, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.40, 126.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285106, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.61, 128.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675164 reads in 391.140 CPU sec, 12.609 real sec
[M::mem_process_seqs] Processed 675102 reads in 274.429 CPU sec, 8.798 real sec
[M::mem_process_seqs] Processed 675074 reads in 286.081 CPU sec, 9.199 real sec
sorting block 134, #reads = 1080423 mem = 536879218 peak = 1287995392
[M::process] read 675156 sequences (100000174 bp) in 2.496 CPU sec, 2.966 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285056, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.64, 128.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 135, #reads = 1081009 mem = 536878444 peak = 1287995392
[M::process] read 675186 sequences (100000289 bp) in 2.813 CPU sec, 2.981 real sec...
[M::process] read 675282 sequences (100000009 bp) in 2.047 CPU sec, 2.126 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285454, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.63, 129.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 18, 82)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 232)
[M::mem_pestat] mean and std.dev: (27.09, 28.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 307)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675138 reads in 268.761 CPU sec, 8.660 real sec
[M::mem_process_seqs] Processed 675156 reads in 150.688 CPU sec, 4.784 real sec
sorting block 136, #reads = 1080537 mem = 536881408 peak = 1287995392
[M::process] read 674950 sequences (100000132 bp) in 2.848 CPU sec, 3.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285132, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.77, 128.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674996 sequences (100000102 bp) in 2.007 CPU sec, 2.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285448, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.59, 129.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675186 reads in 264.908 CPU sec, 8.550 real sec
[M::mem_process_seqs] Processed 675282 reads in 147.656 CPU sec, 4.715 real sec
sorting block 137, #reads = 1080355 mem = 536878747 peak = 1287995392
[M::process] read 675156 sequences (100000170 bp) in 2.748 CPU sec, 3.335 real sec...
[M::process] read 674988 sequences (100000084 bp) in 2.181 CPU sec, 2.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285452, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.42, 132.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674996 reads in 141.423 CPU sec, 4.487 real sec
sorting block 138, #reads = 1080395 mem = 536880473 peak = 1287995392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284861, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (259.41, 133.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674896 sequences (100000251 bp) in 2.030 CPU sec, 2.130 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285130, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.55, 130.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (88, 480, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1264)
[M::mem_pestat] mean and std.dev: (312.50, 261.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1656)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674950 reads in 405.308 CPU sec, 13.048 real sec
[M::mem_process_seqs] Processed 675156 reads in 161.390 CPU sec, 5.220 real sec
sorting block 139, #reads = 1080354 mem = 536878681 peak = 1287995392
[M::process] read 674858 sequences (100000282 bp) in 2.880 CPU sec, 3.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285248, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.10, 130.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674898 sequences (100000135 bp) in 2.104 CPU sec, 2.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285257, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.42, 132.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 545, 3151)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9401)
[M::mem_pestat] mean and std.dev: (1042.21, 1348.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12526)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674988 reads in 265.717 CPU sec, 8.534 real sec
[M::mem_process_seqs] Processed 674896 reads in 147.299 CPU sec, 4.708 real sec
sorting block 140, #reads = 1080658 mem = 536879376 peak = 1287995392
[M::process] read 674984 sequences (100000206 bp) in 2.681 CPU sec, 3.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285391, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.35, 133.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 38, 45)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 107)
[M::mem_pestat] mean and std.dev: (29.18, 13.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 138)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674844 sequences (100000095 bp) in 2.098 CPU sec, 2.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285600, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.55, 134.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284808, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.26, 132.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 22, 32)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 76)
[M::mem_pestat] mean and std.dev: (15.80, 7.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 98)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674858 reads in 382.631 CPU sec, 12.301 real sec
sorting block 141, #reads = 1080402 mem = 536877927 peak = 1287995392
[M::mem_process_seqs] Processed 674984 reads in 269.322 CPU sec, 8.632 real sec
[M::mem_process_seqs] Processed 674898 reads in 280.665 CPU sec, 9.017 real sec
sorting block 142, #reads = 1080198 mem = 536881622 peak = 1287995392
[M::process] read 674852 sequences (100000282 bp) in 2.569 CPU sec, 2.890 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285381, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.37, 134.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 2325, 2325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6921)
[M::mem_pestat] mean and std.dev: (1207.42, 1121.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9219)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674878 sequences (100000117 bp) in 2.609 CPU sec, 3.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285336, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.91, 134.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674820 sequences (100000226 bp) in 2.062 CPU sec, 2.065 real sec...
[M::mem_process_seqs] Processed 674844 reads in 264.112 CPU sec, 8.595 real sec
[M::mem_process_seqs] Processed 674852 reads in 149.097 CPU sec, 4.754 real sec
sorting block 143, #reads = 1079365 mem = 536879645 peak = 1287995392
sorting block 144, #reads = 1080236 mem = 536878775 peak = 1287995392
[M::process] read 674894 sequences (100000020 bp) in 2.529 CPU sec, 3.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285321, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.18, 135.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674948 sequences (100000219 bp) in 2.045 CPU sec, 2.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285548, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (258.86, 135.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674878 reads in 265.592 CPU sec, 8.637 real sec
[M::mem_process_seqs] Processed 674820 reads in 147.479 CPU sec, 4.712 real sec
sorting block 145, #reads = 1080077 mem = 536880600 peak = 1287995392
[M::process] read 674900 sequences (100000134 bp) in 2.698 CPU sec, 2.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285100, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.19, 135.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674862 sequences (100000229 bp) in 2.154 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285421, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.55, 136.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 380, 3534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10566)
[M::mem_pestat] mean and std.dev: (1307.62, 1539.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14082)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285312, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.39, 135.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674894 reads in 388.075 CPU sec, 12.477 real sec
[M::mem_process_seqs] Processed 674948 reads in 269.848 CPU sec, 8.663 real sec
[M::mem_process_seqs] Processed 674900 reads in 281.278 CPU sec, 9.050 real sec
sorting block 146, #reads = 1080234 mem = 536879255 peak = 1287995392
[M::process] read 674770 sequences (100000191 bp) in 2.736 CPU sec, 2.887 real sec...
sorting block 147, #reads = 1080728 mem = 536878528 peak = 1287995392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285620, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (260.89, 137.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674812 sequences (100000209 bp) in 2.513 CPU sec, 2.818 real sec...
[M::process] read 675086 sequences (100000098 bp) in 2.178 CPU sec, 2.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285390, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (259.48, 135.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 179, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1444)
[M::mem_pestat] mean and std.dev: (294.22, 398.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1919)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674862 reads in 267.412 CPU sec, 8.590 real sec
[M::mem_process_seqs] Processed 674770 reads in 148.456 CPU sec, 4.716 real sec
sorting block 148, #reads = 1080501 mem = 536878176 peak = 1287995392
[M::process] read 676264 sequences (100000212 bp) in 2.875 CPU sec, 2.990 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285849, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.10, 133.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (100, 596, 725)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1975)
[M::mem_pestat] mean and std.dev: (492.70, 394.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2600)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676120 sequences (100000028 bp) in 2.121 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285918, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (252.48, 130.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 27, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (214.58, 265.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286343, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (235.33, 113.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674812 reads in 360.771 CPU sec, 11.556 real sec
[M::mem_process_seqs] Processed 675086 reads in 253.367 CPU sec, 8.120 real sec
[M::mem_process_seqs] Processed 676264 reads in 264.522 CPU sec, 8.499 real sec
sorting block 149, #reads = 1080065 mem = 536878628 peak = 1287995392
[M::process] read 676028 sequences (100000069 bp) in 2.427 CPU sec, 2.949 real sec...
sorting block 150, #reads = 1080353 mem = 536879593 peak = 1287995392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286428, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (236.99, 115.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675902 sequences (100000038 bp) in 2.953 CPU sec, 3.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286949, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (237.30, 115.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676120 reads in 244.842 CPU sec, 7.927 real sec
[M::process] read 675988 sequences (100000172 bp) in 2.145 CPU sec, 2.154 real sec...
[M::mem_process_seqs] Processed 676028 reads in 139.579 CPU sec, 4.425 real sec
sorting block 151, #reads = 1080811 mem = 536879336 peak = 1287995392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286807, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (238.98, 117.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 385, 612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1546)
[M::mem_pestat] mean and std.dev: (330.29, 244.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2013)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675934 sequences (100000102 bp) in 2.528 CPU sec, 3.042 real sec...
[M::process] read 675824 sequences (100000020 bp) in 2.068 CPU sec, 2.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286721, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.01, 117.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675902 reads in 242.617 CPU sec, 7.853 real sec
sorting block 152, #reads = 1081027 mem = 536878032 peak = 1287995392
[M::process] read 675864 sequences (100000084 bp) in 2.107 CPU sec, 2.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286560, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (239.46, 118.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675934 reads in 130.259 CPU sec, 4.253 real sec
[M::mem_process_seqs] Processed 675988 reads in 316.931 CPU sec, 10.224 real sec
sorting block 153, #reads = 1081346 mem = 536880085 peak = 1287995392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286633, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (241.18, 119.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675842 sequences (100000089 bp) in 2.427 CPU sec, 2.979 real sec...
[M::process] read 675504 sequences (100000128 bp) in 2.139 CPU sec, 2.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286664, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.28, 120.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675824 reads in 258.675 CPU sec, 8.319 real sec
[M::mem_process_seqs] Processed 675864 reads in 141.012 CPU sec, 4.489 real sec
sorting block 154, #reads = 1081128 mem = 536877740 peak = 1287995392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286125, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (242.85, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 24, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (77.25, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675632 sequences (100000063 bp) in 2.469 CPU sec, 3.191 real sec...
[M::process] read 675558 sequences (100000063 bp) in 2.045 CPU sec, 2.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286571, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (245.50, 122.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675842 reads in 242.004 CPU sec, 7.864 real sec
[M::mem_process_seqs] Processed 675504 reads in 135.779 CPU sec, 4.328 real sec
sorting block 155, #reads = 1081383 mem = 536878971 peak = 1287995392
sorting block 156, #reads = 1080618 mem = 536879237 peak = 1287995392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286037, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (246.48, 123.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675544 sequences (100000028 bp) in 2.482 CPU sec, 2.989 real sec...
[M::process] read 675520 sequences (100000298 bp) in 2.078 CPU sec, 2.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285918, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (246.74, 123.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 14, 50)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 140)
[M::mem_pestat] mean and std.dev: (13.09, 12.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 185)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675632 reads in 249.116 CPU sec, 8.042 real sec
[M::mem_process_seqs] Processed 675558 reads in 139.415 CPU sec, 4.447 real sec
sorting block 157, #reads = 1081268 mem = 536881495 peak = 1287995392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286688, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (246.63, 123.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675452 sequences (100000018 bp) in 2.361 CPU sec, 2.902 real sec...
[M::process] read 675432 sequences (100000141 bp) in 1.892 CPU sec, 1.896 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286385, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (246.63, 123.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 352, 933)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2779)
[M::mem_pestat] mean and std.dev: (579.20, 705.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3702)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675544 reads in 243.091 CPU sec, 7.893 real sec
[M::mem_process_seqs] Processed 675520 reads in 137.879 CPU sec, 4.440 real sec
sorting block 158, #reads = 1080853 mem = 536880496 peak = 1287995392
[M::process] read 675468 sequences (100000252 bp) in 2.350 CPU sec, 2.625 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286201, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (247.34, 124.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 17, 32)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 78)
[M::mem_pestat] mean and std.dev: (15.71, 11.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 101)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675512 sequences (100000048 bp) in 2.116 CPU sec, 2.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286033, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (248.91, 125.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 217, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (236.23, 251.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286059, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (250.51, 126.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675452 reads in 369.549 CPU sec, 11.966 real sec
[M::mem_process_seqs] Processed 675432 reads in 260.570 CPU sec, 8.409 real sec
[M::mem_process_seqs] Processed 675468 reads in 271.633 CPU sec, 8.784 real sec
sorting block 159, #reads = 1080666 mem = 536877833 peak = 1287995392
[M::process] read 675304 sequences (100000266 bp) in 2.573 CPU sec, 2.962 real sec...
sorting block 160, #reads = 1080656 mem = 536880496 peak = 1288245248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286150, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.02, 126.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 46, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (175.50, 236.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675262 sequences (100000046 bp) in 2.758 CPU sec, 2.942 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286274, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.51, 125.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 100, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (68.10, 78.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675398 sequences (100000129 bp) in 1.846 CPU sec, 1.890 real sec...
[M::mem_process_seqs] Processed 675512 reads in 250.860 CPU sec, 8.099 real sec
[M::mem_process_seqs] Processed 675304 reads in 140.570 CPU sec, 4.477 real sec
sorting block 161, #reads = 1080271 mem = 536878111 peak = 1288245248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286145, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (249.91, 126.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 58, 65)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191)
[M::mem_pestat] mean and std.dev: (28.44, 29.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 254)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675320 sequences (100000252 bp) in 2.829 CPU sec, 3.312 real sec...
[M::process] read 675252 sequences (100000253 bp) in 2.060 CPU sec, 2.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286078, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (248.19, 125.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675262 reads in 253.177 CPU sec, 8.183 real sec
[M::mem_process_seqs] Processed 675398 reads in 144.540 CPU sec, 4.590 real sec
sorting block 162, #reads = 1080767 mem = 536877947 peak = 1288245248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286277, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (251.90, 128.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 61, 88)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 142)
[M::mem_pestat] mean and std.dev: (56.00, 19.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 169)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 163, #reads = 1080829 mem = 536878281 peak = 1288245248
[M::process] read 675310 sequences (100000123 bp) in 2.780 CPU sec, 3.282 real sec...
[M::process] read 675284 sequences (100000123 bp) in 2.062 CPU sec, 2.069 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285372, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (251.39, 127.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675320 reads in 255.165 CPU sec, 8.222 real sec
[M::mem_process_seqs] Processed 675252 reads in 145.303 CPU sec, 4.629 real sec
sorting block 164, #reads = 1080743 mem = 536878556 peak = 1288245248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285926, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.57, 128.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 37, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1713)
[M::mem_pestat] mean and std.dev: (245.27, 275.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2283)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675172 sequences (100000159 bp) in 2.667 CPU sec, 3.508 real sec...
[M::process] read 675198 sequences (100000163 bp) in 2.172 CPU sec, 2.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286347, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.25, 129.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (53, 288, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (228.12, 199.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1325)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675310 reads in 258.857 CPU sec, 8.418 real sec
[M::mem_process_seqs] Processed 675284 reads in 146.600 CPU sec, 4.677 real sec
sorting block 165, #reads = 1080703 mem = 536881745 peak = 1288245248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285800, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (252.74, 128.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675296 sequences (100000036 bp) in 2.809 CPU sec, 3.278 real sec...
[M::process] read 675160 sequences (100000149 bp) in 2.096 CPU sec, 2.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285505, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.15, 129.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675172 reads in 259.275 CPU sec, 8.345 real sec
[M::mem_process_seqs] Processed 675198 reads in 145.987 CPU sec, 4.649 real sec
sorting block 166, #reads = 1080833 mem = 536877917 peak = 1288245248
[M::process] read 675200 sequences (100000233 bp) in 2.590 CPU sec, 3.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285427, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (253.88, 129.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675116 sequences (100000223 bp) in 2.070 CPU sec, 2.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285399, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.36, 130.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675296 reads in 266.953 CPU sec, 8.689 real sec
[M::mem_process_seqs] Processed 675160 reads in 147.928 CPU sec, 4.722 real sec
sorting block 167, #reads = 1079402 mem = 536878939 peak = 1288245248
sorting block 168, #reads = 1080651 mem = 536882791 peak = 1288245248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285729, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.56, 129.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675152 sequences (100000094 bp) in 2.735 CPU sec, 3.221 real sec...
[M::process] read 675058 sequences (100000264 bp) in 2.067 CPU sec, 2.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285508, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.13, 131.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675200 reads in 264.582 CPU sec, 8.554 real sec
[M::mem_process_seqs] Processed 675116 reads in 150.340 CPU sec, 4.794 real sec
sorting block 169, #reads = 1080703 mem = 536878963 peak = 1288245248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285292, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (254.50, 131.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675138 sequences (100000212 bp) in 3.220 CPU sec, 3.409 real sec...
[M::process] read 675084 sequences (100000241 bp) in 2.193 CPU sec, 2.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285532, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (255.99, 132.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 245, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095)
[M::mem_pestat] mean and std.dev: (191.38, 167.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1459)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675152 reads in 270.169 CPU sec, 8.647 real sec
[M::mem_process_seqs] Processed 675058 reads in 152.186 CPU sec, 4.844 real sec
sorting block 170, #reads = 1079489 mem = 536878647 peak = 1288245248
[M::process] read 674940 sequences (100000181 bp) in 2.683 CPU sec, 3.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285615, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.57, 132.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 360, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1333)
[M::mem_pestat] mean and std.dev: (253.10, 195.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1770)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675026 sequences (100000229 bp) in 2.090 CPU sec, 2.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285311, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (256.44, 132.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675138 reads in 261.028 CPU sec, 8.390 real sec
[M::mem_process_seqs] Processed 675084 reads in 148.161 CPU sec, 4.730 real sec
sorting block 171, #reads = 1079441 mem = 536878980 peak = 1288245248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285565, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.38, 133.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674960 sequences (100000040 bp) in 2.752 CPU sec, 3.331 real sec...
[M::process] read 675040 sequences (100000175 bp) in 2.083 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285520, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.91, 134.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (634, 634, 3687)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9793)
[M::mem_pestat] mean and std.dev: (2542.83, 3360.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15983)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674940 reads in 257.727 CPU sec, 8.299 real sec
[M::mem_process_seqs] Processed 675026 reads in 145.210 CPU sec, 4.631 real sec
sorting block 172, #reads = 1080440 mem = 536881369 peak = 1288245248
sorting block 173, #reads = 1080350 mem = 536881125 peak = 1288245248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285552, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.05, 134.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674988 sequences (100000264 bp) in 2.715 CPU sec, 3.401 real sec...
[M::process] read 674894 sequences (100000230 bp) in 2.139 CPU sec, 2.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285756, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.40, 134.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 42, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199)
[M::mem_pestat] mean and std.dev: (130.92, 223.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1588)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674960 reads in 257.732 CPU sec, 8.301 real sec
[M::mem_process_seqs] Processed 675040 reads in 144.845 CPU sec, 4.615 real sec
sorting block 174, #reads = 1080504 mem = 536880070 peak = 1288245248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285361, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.34, 134.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674918 sequences (100000115 bp) in 2.823 CPU sec, 3.654 real sec...
[M::process] read 674994 sequences (100000009 bp) in 2.138 CPU sec, 2.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285647, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.38, 134.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674988 reads in 259.590 CPU sec, 8.385 real sec
[M::mem_process_seqs] Processed 674894 reads in 146.743 CPU sec, 4.662 real sec
sorting block 175, #reads = 1080548 mem = 536877502 peak = 1288245248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285481, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (259.91, 136.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674974 sequences (100000023 bp) in 2.766 CPU sec, 3.202 real sec...
[M::process] read 675136 sequences (100000262 bp) in 2.140 CPU sec, 2.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285606, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (258.08, 135.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674918 reads in 254.617 CPU sec, 8.208 real sec
[M::mem_process_seqs] Processed 674994 reads in 143.811 CPU sec, 4.575 real sec
sorting block 176, #reads = 1080203 mem = 536878515 peak = 1288245248
[M::process] read 675694 sequences (100000206 bp) in 2.576 CPU sec, 2.749 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285426, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (255.35, 131.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676372 sequences (100000219 bp) in 2.125 CPU sec, 2.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285897, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (254.30, 132.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 42, 1432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4270)
[M::mem_pestat] mean and std.dev: (653.27, 819.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5689)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286422, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (243.68, 122.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674974 reads in 371.297 CPU sec, 11.915 real sec
sorting block 177, #reads = 1080310 mem = 536878537 peak = 1288245248
[M::mem_process_seqs] Processed 675136 reads in 260.725 CPU sec, 8.367 real sec
[M::mem_process_seqs] Processed 675694 reads in 272.205 CPU sec, 8.757 real sec
sorting block 178, #reads = 1079885 mem = 536880529 peak = 1293295616
[M::process] read 676346 sequences (100000179 bp) in 2.761 CPU sec, 3.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286262, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (233.11, 112.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676286 sequences (100000141 bp) in 2.799 CPU sec, 3.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286632, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (235.19, 114.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676372 reads in 257.549 CPU sec, 8.393 real sec
[M::mem_process_seqs] Processed 676346 reads in 147.468 CPU sec, 4.716 real sec
sorting block 179, #reads = 1080629 mem = 536877794 peak = 1293295616
[M::process] read 676072 sequences (100000070 bp) in 2.461 CPU sec, 2.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286513, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (236.49, 115.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 53, 190)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544)
[M::mem_pestat] mean and std.dev: (79.45, 78.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 180, #reads = 1081206 mem = 536879667 peak = 1293295616
[M::process] read 675938 sequences (100000194 bp) in 2.699 CPU sec, 3.258 real sec...
[M::process] read 676062 sequences (100000257 bp) in 2.125 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286670, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (238.29, 117.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676286 reads in 250.368 CPU sec, 8.074 real sec
[M::mem_process_seqs] Processed 676072 reads in 141.871 CPU sec, 4.529 real sec
sorting block 181, #reads = 1081026 mem = 536880848 peak = 1293295616
[M::process] read 676110 sequences (100000214 bp) in 2.634 CPU sec, 2.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285975, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (238.07, 116.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (606, 606, 1054)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1950)
[M::mem_pestat] mean and std.dev: (830.10, 522.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2920)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675802 sequences (100000174 bp) in 2.157 CPU sec, 2.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286373, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (238.03, 116.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286299, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.07, 117.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 974, 974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2900)
[M::mem_pestat] mean and std.dev: (559.44, 470.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3863)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675938 reads in 373.435 CPU sec, 11.985 real sec
[M::mem_process_seqs] Processed 676062 reads in 262.318 CPU sec, 8.409 real sec
[M::mem_process_seqs] Processed 676110 reads in 273.614 CPU sec, 8.794 real sec
sorting block 182, #reads = 1080894 mem = 536881359 peak = 1293295616
[M::process] read 675806 sequences (100000217 bp) in 2.933 CPU sec, 3.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286156, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.65, 120.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (88, 104, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (175.40, 155.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 183, #reads = 1080754 mem = 536879618 peak = 1293295616
[M::process] read 675764 sequences (100000190 bp) in 2.869 CPU sec, 3.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286352, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.86, 121.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675802 reads in 261.051 CPU sec, 8.490 real sec
[M::process] read 675768 sequences (100000005 bp) in 2.154 CPU sec, 2.159 real sec...
[M::mem_process_seqs] Processed 675806 reads in 147.051 CPU sec, 4.727 real sec
sorting block 184, #reads = 1080716 mem = 536879538 peak = 1293295616
[M::process] read 675654 sequences (100000199 bp) in 2.849 CPU sec, 3.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286090, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.69, 121.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 50, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271)
[M::mem_pestat] mean and std.dev: (176.92, 215.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1686)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675648 sequences (100000231 bp) in 2.107 CPU sec, 2.114 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286144, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.16, 121.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675764 reads in 256.664 CPU sec, 8.228 real sec
[M::mem_process_seqs] Processed 675768 reads in 143.044 CPU sec, 4.565 real sec
sorting block 185, #reads = 1081215 mem = 536881022 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285547, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.60, 121.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675520 sequences (100000042 bp) in 2.714 CPU sec, 3.467 real sec...
[M::process] read 675602 sequences (100000013 bp) in 2.156 CPU sec, 2.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285482, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (245.37, 123.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675654 reads in 259.899 CPU sec, 8.403 real sec
[M::mem_process_seqs] Processed 675648 reads in 146.163 CPU sec, 4.645 real sec
sorting block 186, #reads = 1080781 mem = 536879811 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286080, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (246.36, 123.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675516 sequences (100000021 bp) in 2.838 CPU sec, 3.518 real sec...
[M::process] read 675462 sequences (100000202 bp) in 2.108 CPU sec, 2.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286113, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (246.83, 124.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675520 reads in 261.094 CPU sec, 8.415 real sec
sorting block 187, #reads = 1080428 mem = 536877327 peak = 1293295616
[M::mem_process_seqs] Processed 675602 reads in 146.570 CPU sec, 4.655 real sec
sorting block 188, #reads = 1081096 mem = 536879211 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285768, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.25, 126.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675380 sequences (100000046 bp) in 2.841 CPU sec, 3.602 real sec...
[M::process] read 675316 sequences (100000107 bp) in 2.035 CPU sec, 2.047 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286067, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (249.90, 126.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675516 reads in 263.552 CPU sec, 8.597 real sec
[M::mem_process_seqs] Processed 675462 reads in 146.873 CPU sec, 4.740 real sec
sorting block 189, #reads = 1081073 mem = 536879704 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285491, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.54, 125.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675326 sequences (100000292 bp) in 2.877 CPU sec, 3.633 real sec...
[M::process] read 675290 sequences (100000207 bp) in 2.127 CPU sec, 2.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285954, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.68, 126.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675380 reads in 259.789 CPU sec, 8.428 real sec
[M::mem_process_seqs] Processed 675316 reads in 145.619 CPU sec, 4.681 real sec
sorting block 190, #reads = 1080487 mem = 536881206 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285939, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.62, 125.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 23, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (101.55, 107.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675278 sequences (100000037 bp) in 3.168 CPU sec, 3.456 real sec...
[M::process] read 675434 sequences (100000002 bp) in 2.171 CPU sec, 2.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285832, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (251.81, 128.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675326 reads in 259.387 CPU sec, 8.326 real sec
[M::mem_process_seqs] Processed 675290 reads in 145.340 CPU sec, 4.626 real sec
sorting block 191, #reads = 1081115 mem = 536878611 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285573, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.40, 128.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 192, #reads = 1080850 mem = 536878005 peak = 1293295616
[M::process] read 675262 sequences (100000072 bp) in 2.760 CPU sec, 3.418 real sec...
[M::process] read 675280 sequences (100000208 bp) in 2.120 CPU sec, 2.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285160, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.53, 128.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675278 reads in 267.016 CPU sec, 8.675 real sec
[M::mem_process_seqs] Processed 675434 reads in 151.905 CPU sec, 4.867 real sec
sorting block 193, #reads = 1080008 mem = 536879143 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285546, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.46, 127.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675198 sequences (100000255 bp) in 3.148 CPU sec, 3.476 real sec...
[M::process] read 675248 sequences (100000024 bp) in 2.403 CPU sec, 2.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286154, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (251.99, 128.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 626, 1109)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3131)
[M::mem_pestat] mean and std.dev: (809.77, 811.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4142)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675262 reads in 267.207 CPU sec, 8.627 real sec
[M::mem_process_seqs] Processed 675280 reads in 148.222 CPU sec, 4.762 real sec
sorting block 194, #reads = 1080977 mem = 536879218 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285320, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.73, 129.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675106 sequences (100000192 bp) in 3.333 CPU sec, 3.662 real sec...
[M::process] read 675184 sequences (100000008 bp) in 2.203 CPU sec, 2.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285468, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.08, 128.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675198 reads in 266.974 CPU sec, 8.539 real sec
[M::mem_process_seqs] Processed 675248 reads in 148.951 CPU sec, 4.743 real sec
sorting block 195, #reads = 1080337 mem = 536879055 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285615, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.53, 129.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 1750, 8664)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25942)
[M::mem_pestat] mean and std.dev: (3486.21, 3913.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 34581)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675182 sequences (100000132 bp) in 2.848 CPU sec, 3.560 real sec...
[M::process] read 675068 sequences (100000147 bp) in 2.158 CPU sec, 2.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285530, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.34, 130.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 105, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1996)
[M::mem_pestat] mean and std.dev: (248.80, 299.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2657)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675106 reads in 264.532 CPU sec, 8.557 real sec
[M::mem_process_seqs] Processed 675184 reads in 150.404 CPU sec, 4.823 real sec
sorting block 196, #reads = 1080287 mem = 536878016 peak = 1293295616
sorting block 197, #reads = 1080393 mem = 536878131 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285449, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.29, 131.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 128, 968)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2702)
[M::mem_pestat] mean and std.dev: (276.25, 338.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3569)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675036 sequences (100000276 bp) in 2.952 CPU sec, 3.586 real sec...
[M::process] read 675026 sequences (100000053 bp) in 2.163 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284706, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (255.89, 132.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675182 reads in 267.573 CPU sec, 8.635 real sec
[M::mem_process_seqs] Processed 675068 reads in 150.220 CPU sec, 4.789 real sec
sorting block 198, #reads = 1080410 mem = 536879887 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284910, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.37, 131.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675068 sequences (100000088 bp) in 3.087 CPU sec, 3.240 real sec...
[M::process] read 675062 sequences (100000207 bp) in 2.205 CPU sec, 2.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285573, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.56, 132.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675036 reads in 264.531 CPU sec, 8.477 real sec
[M::mem_process_seqs] Processed 675026 reads in 147.459 CPU sec, 4.701 real sec
sorting block 199, #reads = 1080134 mem = 536878403 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285584, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.16, 133.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675038 sequences (100000273 bp) in 2.888 CPU sec, 3.579 real sec...
[M::process] read 675048 sequences (100000174 bp) in 2.337 CPU sec, 2.345 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285258, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.67, 133.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 367, 1758)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5018)
[M::mem_pestat] mean and std.dev: (835.36, 778.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6648)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675068 reads in 264.331 CPU sec, 8.509 real sec
[M::mem_process_seqs] Processed 675062 reads in 149.955 CPU sec, 4.783 real sec
sorting block 200, #reads = 1080710 mem = 536877226 peak = 1293295616
[M::process] read 675152 sequences (100000157 bp) in 2.958 CPU sec, 3.743 real sec...
[M::process] read 675104 sequences (100000066 bp) in 2.251 CPU sec, 2.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285367, 26, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.98, 134.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 70, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 197)
[M::mem_pestat] mean and std.dev: (43.45, 26.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 259)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675048 reads in 138.366 CPU sec, 4.374 real sec
sorting block 201, #reads = 1079799 mem = 536878011 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285533, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.71, 133.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285831, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (256.76, 133.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675076 sequences (100000204 bp) in 2.206 CPU sec, 2.629 real sec...
[M::mem_process_seqs] Processed 675038 reads in 399.578 CPU sec, 12.851 real sec
[M::mem_process_seqs] Processed 675152 reads in 159.330 CPU sec, 5.137 real sec
sorting block 202, #reads = 1080644 mem = 536878129 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285084, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.45, 133.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674936 sequences (100000053 bp) in 3.400 CPU sec, 3.754 real sec...
[M::process] read 674968 sequences (100000111 bp) in 2.304 CPU sec, 2.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284711, 21, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.62, 134.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 73, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (76.67, 107.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1432)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675104 reads in 268.657 CPU sec, 8.627 real sec
[M::mem_process_seqs] Processed 675076 reads in 152.668 CPU sec, 4.867 real sec
sorting block 203, #reads = 1080626 mem = 536878165 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285706, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (258.63, 136.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675046 sequences (100000198 bp) in 2.738 CPU sec, 3.406 real sec...
sorting block 204, #reads = 1080279 mem = 536879380 peak = 1293295616
[M::process] read 675166 sequences (100000110 bp) in 2.244 CPU sec, 2.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285533, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.18, 134.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674936 reads in 262.419 CPU sec, 8.544 real sec
[M::mem_process_seqs] Processed 674968 reads in 150.534 CPU sec, 4.841 real sec
sorting block 205, #reads = 1080296 mem = 536878913 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285426, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.30, 132.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (90, 229, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (199.11, 120.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675392 sequences (100000114 bp) in 3.062 CPU sec, 3.730 real sec...
[M::process] read 676426 sequences (100000283 bp) in 2.206 CPU sec, 2.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286159, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (253.49, 131.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 2077, 2620)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7838)
[M::mem_pestat] mean and std.dev: (1261.10, 1167.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10447)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675046 reads in 258.667 CPU sec, 8.373 real sec
[M::mem_process_seqs] Processed 675166 reads in 145.662 CPU sec, 4.685 real sec
sorting block 206, #reads = 1079974 mem = 536880368 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286466, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (248.22, 126.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676338 sequences (100000124 bp) in 2.684 CPU sec, 2.867 real sec...
[M::process] read 676180 sequences (100000279 bp) in 2.185 CPU sec, 2.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286844, 17, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (232.20, 111.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 69, 738)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2168)
[M::mem_pestat] mean and std.dev: (257.35, 319.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2883)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675392 reads in 249.005 CPU sec, 8.010 real sec
[M::mem_process_seqs] Processed 676426 reads in 142.038 CPU sec, 4.548 real sec
sorting block 207, #reads = 1080080 mem = 536879154 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286780, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (235.07, 113.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676150 sequences (100000037 bp) in 2.884 CPU sec, 3.706 real sec...
[M::process] read 675976 sequences (100000102 bp) in 2.216 CPU sec, 2.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286057, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (236.49, 114.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676338 reads in 255.052 CPU sec, 8.266 real sec
[M::mem_process_seqs] Processed 676180 reads in 145.721 CPU sec, 4.658 real sec
sorting block 208, #reads = 1081546 mem = 536878553 peak = 1293295616
sorting block 209, #reads = 1081241 mem = 536877615 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286222, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (237.88, 116.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676054 sequences (100000101 bp) in 2.675 CPU sec, 2.922 real sec...
[M::process] read 676100 sequences (100000216 bp) in 2.159 CPU sec, 2.165 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286033, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (237.57, 116.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676150 reads in 252.228 CPU sec, 8.110 real sec
[M::mem_process_seqs] Processed 675976 reads in 143.620 CPU sec, 4.590 real sec
sorting block 210, #reads = 1081140 mem = 536880345 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285682, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.42, 117.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 26, 58)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 148)
[M::mem_pestat] mean and std.dev: (28.00, 19.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 193)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675836 sequences (100000072 bp) in 2.804 CPU sec, 3.169 real sec...
[M::process] read 675758 sequences (100000250 bp) in 2.203 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286013, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (240.17, 118.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676054 reads in 258.524 CPU sec, 8.331 real sec
[M::mem_process_seqs] Processed 676100 reads in 144.702 CPU sec, 4.643 real sec
sorting block 211, #reads = 1080599 mem = 536880422 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285877, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (240.16, 118.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 72, 703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2059)
[M::mem_pestat] mean and std.dev: (311.44, 310.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2737)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675804 sequences (100000224 bp) in 2.549 CPU sec, 3.248 real sec...
[M::process] read 675742 sequences (100000029 bp) in 2.372 CPU sec, 2.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286248, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.20, 120.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675836 reads in 255.768 CPU sec, 8.271 real sec
[M::mem_process_seqs] Processed 675758 reads in 145.832 CPU sec, 4.646 real sec
sorting block 212, #reads = 1081156 mem = 536879310 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286006, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.53, 120.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675596 sequences (100000133 bp) in 2.756 CPU sec, 3.045 real sec...
[M::process] read 675684 sequences (100000189 bp) in 2.196 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285875, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.49, 121.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675804 reads in 257.199 CPU sec, 8.251 real sec
[M::mem_process_seqs] Processed 675742 reads in 144.412 CPU sec, 4.596 real sec
sorting block 213, #reads = 1080474 mem = 536877195 peak = 1293295616
sorting block 214, #reads = 1081289 mem = 536882102 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285891, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.57, 121.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675784 sequences (100000281 bp) in 2.679 CPU sec, 3.257 real sec...
[M::process] read 675586 sequences (100000271 bp) in 2.148 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286009, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.76, 121.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 77, 3091)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9239)
[M::mem_pestat] mean and std.dev: (1601.25, 2429.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12313)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675596 reads in 263.171 CPU sec, 8.466 real sec
[M::mem_process_seqs] Processed 675684 reads in 146.874 CPU sec, 4.688 real sec
sorting block 215, #reads = 1080824 mem = 536877165 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286390, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.64, 122.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675478 sequences (100000211 bp) in 3.209 CPU sec, 3.560 real sec...
[M::process] read 675544 sequences (100000285 bp) in 2.243 CPU sec, 2.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286139, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.63, 122.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675784 reads in 263.987 CPU sec, 8.545 real sec
[M::mem_process_seqs] Processed 675586 reads in 148.902 CPU sec, 4.763 real sec
sorting block 216, #reads = 1081022 mem = 536878971 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285298, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (246.45, 124.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675492 sequences (100000159 bp) in 2.737 CPU sec, 3.355 real sec...
[M::process] read 675288 sequences (100000096 bp) in 2.158 CPU sec, 2.165 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284936, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (246.71, 124.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675478 reads in 267.582 CPU sec, 8.629 real sec
[M::mem_process_seqs] Processed 675544 reads in 149.678 CPU sec, 4.766 real sec
sorting block 217, #reads = 1081290 mem = 536880483 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285081, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.57, 124.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675352 sequences (100000224 bp) in 3.172 CPU sec, 3.536 real sec...
[M::process] read 675282 sequences (100000064 bp) in 2.208 CPU sec, 2.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285433, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.07, 125.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 42, 776)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2322)
[M::mem_pestat] mean and std.dev: (149.79, 269.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3095)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675492 reads in 268.506 CPU sec, 8.666 real sec
[M::mem_process_seqs] Processed 675288 reads in 149.560 CPU sec, 4.780 real sec
sorting block 218, #reads = 1080263 mem = 536878432 peak = 1293295616
sorting block 219, #reads = 1080477 mem = 536881009 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285007, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.06, 126.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675262 sequences (100000268 bp) in 2.983 CPU sec, 3.244 real sec...
[M::process] read 675302 sequences (100000178 bp) in 2.291 CPU sec, 2.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284951, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.09, 127.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675352 reads in 271.664 CPU sec, 8.759 real sec
[M::mem_process_seqs] Processed 675282 reads in 151.891 CPU sec, 4.852 real sec
sorting block 220, #reads = 1080334 mem = 536877969 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285641, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.69, 127.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675434 sequences (100000145 bp) in 3.257 CPU sec, 3.438 real sec...
[M::process] read 675370 sequences (100000282 bp) in 2.277 CPU sec, 2.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284866, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.24, 127.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 98, 770)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2172)
[M::mem_pestat] mean and std.dev: (271.42, 296.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2873)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675262 reads in 272.848 CPU sec, 8.752 real sec
[M::mem_process_seqs] Processed 675302 reads in 154.148 CPU sec, 4.893 real sec
sorting block 221, #reads = 1081029 mem = 536878582 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285090, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.62, 126.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675342 sequences (100000247 bp) in 3.021 CPU sec, 3.354 real sec...
[M::process] read 675292 sequences (100000182 bp) in 2.232 CPU sec, 2.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285169, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.49, 128.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (348, 401, 1390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3474)
[M::mem_pestat] mean and std.dev: (789.45, 556.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4516)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675434 reads in 276.531 CPU sec, 8.890 real sec
[M::mem_process_seqs] Processed 675370 reads in 152.505 CPU sec, 4.845 real sec
sorting block 222, #reads = 1080199 mem = 536878376 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285521, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.27, 129.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675222 sequences (100000030 bp) in 3.014 CPU sec, 3.610 real sec...
[M::process] read 675094 sequences (100000227 bp) in 2.211 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285539, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.16, 129.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675342 reads in 266.376 CPU sec, 8.587 real sec
sorting block 223, #reads = 1080774 mem = 536881563 peak = 1293295616
[M::mem_process_seqs] Processed 675292 reads in 148.325 CPU sec, 4.748 real sec
sorting block 224, #reads = 1080084 mem = 536882905 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285133, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.50, 129.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 35, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137)
[M::mem_pestat] mean and std.dev: (140.67, 167.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1511)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675142 sequences (100000146 bp) in 2.976 CPU sec, 3.333 real sec...
[M::process] read 675116 sequences (100000042 bp) in 2.242 CPU sec, 2.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285081, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.69, 130.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675222 reads in 271.208 CPU sec, 8.708 real sec
[M::mem_process_seqs] Processed 675094 reads in 151.986 CPU sec, 4.834 real sec
sorting block 225, #reads = 1080942 mem = 536877229 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285324, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.12, 132.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675146 sequences (100000027 bp) in 2.628 CPU sec, 3.545 real sec...
[M::process] read 675130 sequences (100000164 bp) in 2.246 CPU sec, 2.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285110, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.65, 131.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675142 reads in 271.755 CPU sec, 8.818 real sec
[M::mem_process_seqs] Processed 675116 reads in 151.943 CPU sec, 4.855 real sec
sorting block 226, #reads = 1080527 mem = 536877896 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284554, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.63, 132.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674982 sequences (100000019 bp) in 2.827 CPU sec, 3.272 real sec...
[M::process] read 674922 sequences (100000209 bp) in 2.267 CPU sec, 2.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285371, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.86, 132.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 225, 738)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1776)
[M::mem_pestat] mean and std.dev: (432.80, 379.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2295)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675146 reads in 276.809 CPU sec, 8.923 real sec
[M::mem_process_seqs] Processed 675130 reads in 152.731 CPU sec, 4.870 real sec
sorting block 227, #reads = 1080460 mem = 536878731 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285045, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.35, 134.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674894 sequences (100000051 bp) in 3.275 CPU sec, 3.474 real sec...
sorting block 228, #reads = 1080310 mem = 536878714 peak = 1293295616
[M::process] read 675068 sequences (100000018 bp) in 2.430 CPU sec, 2.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284804, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (260.06, 136.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 420, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (302.43, 147.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674982 reads in 277.094 CPU sec, 9.006 real sec
[M::mem_process_seqs] Processed 674922 reads in 154.808 CPU sec, 5.001 real sec
sorting block 229, #reads = 1080243 mem = 536878676 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284954, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.76, 134.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674978 sequences (100000021 bp) in 2.618 CPU sec, 3.455 real sec...
[M::process] read 674930 sequences (100000011 bp) in 2.199 CPU sec, 2.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285491, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.40, 135.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674894 reads in 271.086 CPU sec, 8.831 real sec
[M::mem_process_seqs] Processed 675068 reads in 151.942 CPU sec, 4.932 real sec
sorting block 230, #reads = 1079934 mem = 536880494 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284846, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.76, 136.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674916 sequences (100000091 bp) in 3.193 CPU sec, 3.664 real sec...
[M::process] read 674972 sequences (100000291 bp) in 2.283 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285070, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.91, 136.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674978 reads in 274.792 CPU sec, 8.865 real sec
[M::mem_process_seqs] Processed 674930 reads in 152.443 CPU sec, 4.874 real sec
sorting block 231, #reads = 1081249 mem = 536878448 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285394, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (260.26, 136.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 179, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (184.27, 188.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674958 sequences (100000026 bp) in 2.578 CPU sec, 3.265 real sec...
[M::process] read 674878 sequences (100000119 bp) in 2.176 CPU sec, 2.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285045, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (260.41, 136.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 215, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1511)
[M::mem_pestat] mean and std.dev: (164.36, 185.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2011)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674916 reads in 267.923 CPU sec, 8.692 real sec
[M::mem_process_seqs] Processed 674972 reads in 151.518 CPU sec, 4.836 real sec
sorting block 232, #reads = 1080082 mem = 536881193 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285308, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (260.05, 136.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 233, #reads = 1080434 mem = 536879238 peak = 1293295616
[M::process] read 674968 sequences (100000020 bp) in 2.951 CPU sec, 3.392 real sec...
[M::process] read 674950 sequences (100000072 bp) in 2.212 CPU sec, 2.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285017, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (260.42, 137.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674958 reads in 270.095 CPU sec, 8.736 real sec
[M::mem_process_seqs] Processed 674878 reads in 153.588 CPU sec, 4.936 real sec
[M::process] read 60080 sequences (8905266 bp) in 0.377 CPU sec, 0.424 real sec...
sorting block 234, #reads = 1080999 mem = 536880546 peak = 1293295616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285094, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (258.93, 136.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284637, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (261.08, 138.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 28, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1202)
[M::mem_pestat] mean and std.dev: (239.91, 340.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1603)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 25326, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (265.91, 141.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674968 reads in 270.696 CPU sec, 8.834 real sec
[M::mem_process_seqs] Processed 674950 reads in 157.961 CPU sec, 5.105 real sec
[M::mem_process_seqs] Processed 60080 reads in 158.938 CPU sec, 5.141 real sec
sorting block 235, #reads = 1080173 mem = 536879004 peak = 1293295616
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 253258290
overall: 69962915840 mem 51926.638 user 627.736 sys 1731.039 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003322.L008\tPU:L008\tSM:T21-D0003322\tLB:T21-D0003322\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 T21-D0003322_R1.fastq.gz T21-D0003322_R2.fastq.gz
[main] Real time: 1731.078 sec; CPU: 52554.381 sec
sorting block 236, #reads = 677793 mem = 337119840 peak = 1293295616
uniq header count 1
merging 237 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31 max 96
algo: util-sort
license: sentieon:util=1
output file size: 8554919318
output reads: 255674981
bam_mem_sort: 237 calls 279.011 user 5.820 sys 293.464 real
bam_write: 237 calls 183.915 user 12.337 sys 391.027 real
execute: 1 calls 82.078 user 4.135 sys 1828.231 real
merge_files: 1 calls 82.052 user 4.089 sys 96.184 real
parse_chunk: 3787 calls 345.504 user 63.127 sys 495.957 real
read_chunk: 10190 calls 151.955 user 72.372 sys 1698.888 real
sort_block: 1 calls 101.307 user 71.532 sys 1637.724 real
write_chunk: 6401 calls 81.056 user 2.528 sys 90.693 real
overall: 3010752512 mem 3900.310 user 188.053 sys 1828.252 real
ls: cannot access '*.cram': No such file or directory