File Info

Filename
.command.log
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b0/1f36766fa0f9868faceeb6f8fd381f/.command.log
Size
126.6 KB
Attempt
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b0/1f36766fa0f9868faceeb6f8fd381f/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5b/47dac41942ca3c98da2cf8107c6a81/T18-D0003255/0002.T18-D0003255_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5b/47dac41942ca3c98da2cf8107c6a81/T18-D0003255/0002.T18-D0003255_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b0/1f36766fa0f9868faceeb6f8fd381f/.command.run
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2047.657 CPU sec, 71.345 real sec
[M::process] read 673376 sequences (100000050 bp) in 0.881 CPU sec, 0.888 real sec...
[M::process] read 673024 sequences (100000298 bp) in 1.030 CPU sec, 1.023 real sec...
[M::process] read 673054 sequences (100000097 bp) in 1.092 CPU sec, 1.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286217, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.18, 131.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672984 sequences (100000177 bp) in 1.039 CPU sec, 1.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286060, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (269.95, 137.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285770, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.22, 137.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 369, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1378)
[M::mem_pestat] mean and std.dev: (293.62, 141.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1773)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673376 reads in 285.933 CPU sec, 9.212 real sec
[M::mem_process_seqs] Processed 673024 reads in 208.113 CPU sec, 6.796 real sec
[M::mem_process_seqs] Processed 673054 reads in 222.983 CPU sec, 7.330 real sec
sorting block 0, #reads = 1080189 mem = 536879446 peak = 581771264
[M::process] read 672830 sequences (100000115 bp) in 1.495 CPU sec, 1.653 real sec...
[M::process] read 672622 sequences (100000058 bp) in 1.447 CPU sec, 1.569 real sec...
[M::process] read 672608 sequences (100000228 bp) in 0.970 CPU sec, 0.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285261, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.02, 144.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672830 reads in 107.823 CPU sec, 3.467 real sec
sorting block 1, #reads = 1079252 mem = 536878049 peak = 764223488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285363, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.60, 140.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672476 sequences (100000119 bp) in 0.962 CPU sec, 0.963 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285036, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (282.33, 146.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 238, 750)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1992)
[M::mem_pestat] mean and std.dev: (415.17, 456.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2613)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672984 reads in 314.848 CPU sec, 10.143 real sec
[M::mem_process_seqs] Processed 672622 reads in 141.362 CPU sec, 4.624 real sec
sorting block 2, #reads = 1079715 mem = 536877977 peak = 823513088
[M::process] read 672746 sequences (100000009 bp) in 1.651 CPU sec, 1.792 real sec...
[M::process] read 672858 sequences (100000276 bp) in 0.960 CPU sec, 0.962 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285574, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (287.15, 151.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 39, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (97.90, 132.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285174, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (286.49, 150.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285210, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.88, 148.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672608 reads in 298.444 CPU sec, 9.606 real sec
[M::mem_process_seqs] Processed 672476 reads in 213.285 CPU sec, 6.964 real sec
sorting block 3, #reads = 1079970 mem = 536879270 peak = 823513088
[M::mem_process_seqs] Processed 672746 reads in 223.476 CPU sec, 7.335 real sec
sorting block 4, #reads = 1079381 mem = 536880693 peak = 838828032
[M::process] read 673116 sequences (100000018 bp) in 1.837 CPU sec, 2.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285363, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.02, 145.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673208 sequences (100000086 bp) in 1.403 CPU sec, 1.492 real sec...
[M::process] read 673182 sequences (100000008 bp) in 0.990 CPU sec, 0.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285595, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.39, 136.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 422, 1281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3837)
[M::mem_pestat] mean and std.dev: (649.55, 644.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5115)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672858 reads in 207.359 CPU sec, 6.680 real sec
[M::mem_process_seqs] Processed 673116 reads in 120.385 CPU sec, 3.894 real sec
sorting block 5, #reads = 1079498 mem = 536879026 peak = 895356928
[M::process] read 673040 sequences (100000227 bp) in 1.636 CPU sec, 1.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285293, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.33, 138.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 33, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (34.50, 44.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 571)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672894 sequences (100000220 bp) in 1.000 CPU sec, 0.997 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285360, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.92, 142.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (276, 1122, 1135)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2853)
[M::mem_pestat] mean and std.dev: (801.50, 412.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3712)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285127, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.46, 140.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673208 reads in 298.863 CPU sec, 9.628 real sec
sorting block 6, #reads = 1080014 mem = 536879539 peak = 895356928
[M::mem_process_seqs] Processed 673040 reads in 216.118 CPU sec, 7.023 real sec
[M::mem_process_seqs] Processed 673182 reads in 229.319 CPU sec, 7.497 real sec
sorting block 7, #reads = 1080003 mem = 536879725 peak = 895356928
[M::process] read 672780 sequences (100000220 bp) in 1.905 CPU sec, 2.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285181, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.41, 143.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 754, 1070)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3140)
[M::mem_pestat] mean and std.dev: (610.80, 607.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4175)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672972 sequences (100000071 bp) in 1.235 CPU sec, 1.264 real sec...
[M::process] read 673214 sequences (100000205 bp) in 1.010 CPU sec, 1.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285426, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.41, 146.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (48, 552, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1560)
[M::mem_pestat] mean and std.dev: (383.30, 340.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2064)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672894 reads in 204.474 CPU sec, 6.568 real sec
[M::mem_process_seqs] Processed 672780 reads in 114.995 CPU sec, 3.713 real sec
sorting block 8, #reads = 1079990 mem = 536879463 peak = 895356928
sorting block 9, #reads = 1079196 mem = 536879279 peak = 895356928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285779, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 673040 sequences (100000175 bp) in 2.336 CPU sec, 2.600 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.75, 143.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 71, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (99.58, 83.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 680)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672894 sequences (100000296 bp) in 1.052 CPU sec, 1.056 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286288, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (266.28, 133.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 47, 1060)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3086)
[M::mem_pestat] mean and std.dev: (447.36, 563.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4099)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672972 reads in 197.402 CPU sec, 6.424 real sec
[M::mem_process_seqs] Processed 673214 reads in 120.759 CPU sec, 4.033 real sec
sorting block 10, #reads = 1079387 mem = 536878044 peak = 895356928
[M::process] read 673044 sequences (100000163 bp) in 1.808 CPU sec, 2.039 real sec...
[M::process] read 672930 sequences (100000053 bp) in 1.080 CPU sec, 1.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285274, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (274.08, 140.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 211, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583)
[M::mem_pestat] mean and std.dev: (207.43, 136.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672894 reads in 109.144 CPU sec, 3.558 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285638, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.64, 139.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672692 sequences (100000255 bp) in 1.089 CPU sec, 1.094 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284881, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.63, 140.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673040 reads in 306.923 CPU sec, 9.925 real sec
sorting block 11, #reads = 1079741 mem = 536876997 peak = 895356928
[M::mem_process_seqs] Processed 673044 reads in 228.828 CPU sec, 7.457 real sec
sorting block 12, #reads = 1079765 mem = 536881509 peak = 895356928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285105, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.30, 144.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672466 sequences (100000007 bp) in 2.226 CPU sec, 2.493 real sec...
[M::process] read 672626 sequences (100000075 bp) in 1.150 CPU sec, 1.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284946, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.51, 148.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (325, 325, 1876)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4978)
[M::mem_pestat] mean and std.dev: (724.30, 833.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6529)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672930 reads in 203.889 CPU sec, 6.568 real sec
[M::mem_process_seqs] Processed 672692 reads in 122.673 CPU sec, 3.989 real sec
sorting block 13, #reads = 1079960 mem = 536877183 peak = 895356928
[M::process] read 672790 sequences (100000177 bp) in 1.676 CPU sec, 1.849 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284578, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 211, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (289.15, 152.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 267, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (181.36, 118.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 678)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672898 sequences (100000109 bp) in 1.120 CPU sec, 1.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284292, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (287.93, 151.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284341, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (283.27, 149.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 424, 4069)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12157)
[M::mem_pestat] mean and std.dev: (1745.25, 1966.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16201)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672466 reads in 308.627 CPU sec, 9.960 real sec
[M::mem_process_seqs] Processed 672626 reads in 222.095 CPU sec, 7.242 real sec
[M::mem_process_seqs] Processed 672790 reads in 236.669 CPU sec, 7.766 real sec
sorting block 14, #reads = 1079527 mem = 536878914 peak = 895356928
[M::process] read 673278 sequences (100000177 bp) in 2.254 CPU sec, 2.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285177, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.74, 144.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 36, 176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (86.73, 83.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 629)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 15, #reads = 1079554 mem = 536879086 peak = 895356928
[M::process] read 673120 sequences (100000011 bp) in 2.165 CPU sec, 2.416 real sec...
[M::process] read 673002 sequences (100000239 bp) in 1.170 CPU sec, 1.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285274, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.05, 133.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672898 reads in 215.694 CPU sec, 6.952 real sec
[M::mem_process_seqs] Processed 673278 reads in 127.606 CPU sec, 4.118 real sec
sorting block 16, #reads = 1079647 mem = 536879752 peak = 895356928
[M::process] read 672914 sequences (100000021 bp) in 1.979 CPU sec, 2.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285085, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.86, 141.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 9, 29)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 81)
[M::mem_pestat] mean and std.dev: (9.82, 10.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 107)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672812 sequences (100000079 bp) in 1.060 CPU sec, 1.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284998, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.71, 144.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285236, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.99, 142.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 172, 630)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1546)
[M::mem_pestat] mean and std.dev: (358.80, 392.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2004)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673120 reads in 301.109 CPU sec, 9.710 real sec
[M::mem_process_seqs] Processed 672914 reads in 217.074 CPU sec, 7.054 real sec
sorting block 17, #reads = 1079625 mem = 536879426 peak = 895356928
[M::mem_process_seqs] Processed 673002 reads in 231.117 CPU sec, 7.559 real sec
[M::process] read 672786 sequences (100000123 bp) in 1.894 CPU sec, 2.076 real sec...
sorting block 18, #reads = 1080040 mem = 536878482 peak = 895356928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284470, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.67, 146.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 79, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (212.14, 223.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1422)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673044 sequences (100000086 bp) in 2.392 CPU sec, 2.754 real sec...
[M::process] read 673186 sequences (100000181 bp) in 1.173 CPU sec, 1.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284881, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.28, 147.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 81, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589)
[M::mem_pestat] mean and std.dev: (60.83, 71.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672812 reads in 212.722 CPU sec, 6.852 real sec
[M::mem_process_seqs] Processed 672786 reads in 126.763 CPU sec, 4.096 real sec
sorting block 19, #reads = 1079715 mem = 536879940 peak = 895356928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285500, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.19, 140.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673236 sequences (100000186 bp) in 2.564 CPU sec, 2.934 real sec...
[M::process] read 673118 sequences (100000244 bp) in 1.105 CPU sec, 1.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285355, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (268.08, 134.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673044 reads in 202.920 CPU sec, 6.527 real sec
[M::mem_process_seqs] Processed 673186 reads in 120.104 CPU sec, 3.906 real sec
sorting block 20, #reads = 1078912 mem = 536879944 peak = 895356928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285806, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.95, 135.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (365, 885, 5053)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14429)
[M::mem_pestat] mean and std.dev: (1973.67, 2132.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19117)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673160 sequences (100000168 bp) in 2.250 CPU sec, 2.464 real sec...
[M::process] read 673054 sequences (100000171 bp) in 1.430 CPU sec, 1.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285506, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (267.62, 134.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 91, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068)
[M::mem_pestat] mean and std.dev: (292.16, 329.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1609)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673236 reads in 200.597 CPU sec, 6.459 real sec
sorting block 21, #reads = 1079789 mem = 536880692 peak = 895356928
[M::mem_process_seqs] Processed 673118 reads in 119.607 CPU sec, 3.884 real sec
sorting block 22, #reads = 1080180 mem = 536880763 peak = 912838656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285857, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.41, 137.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672792 sequences (100000009 bp) in 2.542 CPU sec, 2.805 real sec...
[M::process] read 672640 sequences (100000074 bp) in 1.250 CPU sec, 1.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285185, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.86, 140.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673160 reads in 198.390 CPU sec, 6.374 real sec
[M::mem_process_seqs] Processed 673054 reads in 115.928 CPU sec, 3.756 real sec
sorting block 23, #reads = 1080258 mem = 536880070 peak = 912838656
[M::process] read 672648 sequences (100000112 bp) in 2.199 CPU sec, 2.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284704, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.79, 146.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 90, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583)
[M::mem_pestat] mean and std.dev: (136.36, 119.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 769)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672864 sequences (100000159 bp) in 1.180 CPU sec, 1.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284434, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (283.46, 148.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 79, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (75.62, 81.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284685, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (282.11, 146.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (359, 359, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (51, 821)
[M::mem_pestat] mean and std.dev: (379.71, 106.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672792 reads in 291.663 CPU sec, 9.404 real sec
[M::mem_process_seqs] Processed 672648 reads in 205.396 CPU sec, 6.685 real sec
[M::mem_process_seqs] Processed 672640 reads in 216.637 CPU sec, 7.254 real sec
sorting block 24, #reads = 1079716 mem = 536877874 peak = 912838656
[M::process] read 673186 sequences (100000196 bp) in 2.631 CPU sec, 3.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285100, 12, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.54, 147.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 130, 164)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414)
[M::mem_pestat] mean and std.dev: (91.30, 55.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 539)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 25, #reads = 1079863 mem = 536880224 peak = 912838656
[M::process] read 673242 sequences (100000033 bp) in 2.175 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285115, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.79, 136.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672864 reads in 193.855 CPU sec, 6.282 real sec
[M::mem_process_seqs] Processed 673186 reads in 108.475 CPU sec, 3.476 real sec
[M::process] read 672956 sequences (100000163 bp) in 1.779 CPU sec, 1.929 real sec...
sorting block 26, #reads = 1079429 mem = 536878126 peak = 912838656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285306, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.52, 135.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673096 sequences (100000155 bp) in 2.048 CPU sec, 2.287 real sec...
[M::process] read 672864 sequences (100000028 bp) in 1.148 CPU sec, 1.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284815, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (273.33, 141.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673242 reads in 195.426 CPU sec, 6.286 real sec
[M::mem_process_seqs] Processed 672956 reads in 110.393 CPU sec, 3.569 real sec
sorting block 27, #reads = 1079720 mem = 536878796 peak = 912838656
[M::process] read 672898 sequences (100000267 bp) in 2.238 CPU sec, 2.513 real sec...
[M::process] read 672636 sequences (100000250 bp) in 1.310 CPU sec, 1.310 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284407, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.15, 145.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 65, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (90.60, 80.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672864 reads in 102.993 CPU sec, 3.309 real sec
sorting block 28, #reads = 1079515 mem = 536878234 peak = 912838656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285031, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.75, 142.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 50, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (126.00, 180.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673404 sequences (100000244 bp) in 1.244 CPU sec, 1.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284726, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.85, 146.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 350, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084)
[M::mem_pestat] mean and std.dev: (226.00, 171.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1425)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673096 reads in 289.148 CPU sec, 9.310 real sec
[M::mem_process_seqs] Processed 672898 reads in 114.957 CPU sec, 3.732 real sec
sorting block 29, #reads = 1079735 mem = 536880650 peak = 912838656
[M::process] read 673384 sequences (100000288 bp) in 2.057 CPU sec, 2.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284417, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.13, 147.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 102, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (196.60, 223.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673480 sequences (100000175 bp) in 1.320 CPU sec, 1.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285202, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.54, 133.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285235, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (262.83, 131.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 104, 142)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398)
[M::mem_pestat] mean and std.dev: (89.60, 69.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 526)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672636 reads in 270.986 CPU sec, 8.723 real sec
[M::mem_process_seqs] Processed 673404 reads in 188.473 CPU sec, 6.097 real sec
sorting block 30, #reads = 1079313 mem = 536878250 peak = 912838656
[M::mem_process_seqs] Processed 673384 reads in 198.266 CPU sec, 6.602 real sec
[M::process] read 673414 sequences (100000130 bp) in 2.009 CPU sec, 2.187 real sec...
sorting block 31, #reads = 1079453 mem = 536881976 peak = 912838656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285806, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 191, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (258.91, 127.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 872)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 10, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110)
[M::mem_pestat] mean and std.dev: (95.54, 158.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1479)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673262 sequences (100000139 bp) in 2.548 CPU sec, 2.885 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285501, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (260.67, 129.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673480 reads in 188.791 CPU sec, 6.077 real sec
[M::mem_process_seqs] Processed 673414 reads in 105.764 CPU sec, 3.378 real sec
[M::process] read 673054 sequences (100000289 bp) in 1.667 CPU sec, 1.778 real sec...
sorting block 32, #reads = 1079623 mem = 536878562 peak = 912838656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285007, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.73, 133.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 35, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320)
[M::mem_pestat] mean and std.dev: (232.76, 334.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1755)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672936 sequences (100000124 bp) in 2.344 CPU sec, 2.556 real sec...
[M::process] read 672918 sequences (100000104 bp) in 1.200 CPU sec, 1.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285045, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.12, 138.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673262 reads in 187.338 CPU sec, 6.022 real sec
sorting block 33, #reads = 1080314 mem = 536880555 peak = 912838656
[M::mem_process_seqs] Processed 673054 reads in 107.700 CPU sec, 3.476 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285093, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.68, 140.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1079719 mem = 536880831 peak = 912838656
[M::process] read 673062 sequences (100000137 bp) in 2.594 CPU sec, 2.930 real sec...
[M::process] read 673092 sequences (100000060 bp) in 1.330 CPU sec, 1.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284622, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (274.09, 140.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 383, 1814)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5184)
[M::mem_pestat] mean and std.dev: (749.64, 717.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6869)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672936 reads in 187.280 CPU sec, 6.016 real sec
[M::mem_process_seqs] Processed 672918 reads in 104.610 CPU sec, 3.352 real sec
sorting block 35, #reads = 1079954 mem = 536879367 peak = 912838656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285044, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.54, 139.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673280 sequences (100000137 bp) in 2.620 CPU sec, 2.859 real sec...
[M::process] read 673158 sequences (100000090 bp) in 1.340 CPU sec, 1.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285343, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.94, 138.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 120, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (146.08, 139.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1343)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673062 reads in 186.572 CPU sec, 5.976 real sec
[M::mem_process_seqs] Processed 673092 reads in 102.312 CPU sec, 3.284 real sec
sorting block 36, #reads = 1079860 mem = 536880164 peak = 912838656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285400, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.26, 132.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673034 sequences (100000248 bp) in 2.461 CPU sec, 2.738 real sec...
[M::process] read 672968 sequences (100000194 bp) in 1.530 CPU sec, 1.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284971, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.83, 135.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673280 reads in 182.258 CPU sec, 5.825 real sec
[M::mem_process_seqs] Processed 673158 reads in 102.634 CPU sec, 3.280 real sec
sorting block 37, #reads = 1079919 mem = 536879229 peak = 912838656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285330, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.52, 139.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 169, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297)
[M::mem_pestat] mean and std.dev: (179.36, 217.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1728)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672784 sequences (100000075 bp) in 2.571 CPU sec, 2.816 real sec...
[M::process] read 672694 sequences (100000055 bp) in 1.220 CPU sec, 1.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284497, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.65, 142.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 33, 81)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215)
[M::mem_pestat] mean and std.dev: (40.89, 39.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 282)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673034 reads in 189.814 CPU sec, 6.090 real sec
sorting block 38, #reads = 1079973 mem = 536880190 peak = 912838656
[M::process] read 672670 sequences (100000256 bp) in 1.283 CPU sec, 1.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284247, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.64, 144.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672784 reads in 107.775 CPU sec, 3.596 real sec
sorting block 39, #reads = 1079782 mem = 536877534 peak = 935124992
[M::mem_process_seqs] Processed 672968 reads in 240.456 CPU sec, 7.889 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284470, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.46, 147.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673386 sequences (100000013 bp) in 2.584 CPU sec, 2.852 real sec...
[M::process] read 673296 sequences (100000275 bp) in 1.760 CPU sec, 1.763 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285084, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 204, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (281.49, 146.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672694 reads in 205.965 CPU sec, 6.595 real sec
[M::mem_process_seqs] Processed 672670 reads in 111.867 CPU sec, 3.592 real sec
sorting block 40, #reads = 1079316 mem = 536880237 peak = 935124992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285461, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.87, 139.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1079372 mem = 536881014 peak = 935124992
[M::process] read 673072 sequences (100000134 bp) in 3.062 CPU sec, 3.408 real sec...
[M::process] read 673058 sequences (100000068 bp) in 1.640 CPU sec, 1.635 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284544, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.42, 140.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 503, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320)
[M::mem_pestat] mean and std.dev: (451.00, 300.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1703)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673386 reads in 189.647 CPU sec, 6.057 real sec
[M::mem_process_seqs] Processed 673296 reads in 109.003 CPU sec, 3.489 real sec
sorting block 42, #reads = 1079550 mem = 536878956 peak = 935124992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284537, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.87, 140.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 7, 94)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276)
[M::mem_pestat] mean and std.dev: (20.38, 33.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 367)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672988 sequences (100000258 bp) in 2.787 CPU sec, 3.047 real sec...
[M::process] read 672692 sequences (100000280 bp) in 1.430 CPU sec, 1.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285233, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.13, 143.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 169, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (248.44, 244.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673072 reads in 194.524 CPU sec, 6.237 real sec
[M::mem_process_seqs] Processed 673058 reads in 110.899 CPU sec, 3.582 real sec
sorting block 43, #reads = 1079958 mem = 536879587 peak = 935124992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284570, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.37, 146.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672526 sequences (100000159 bp) in 2.609 CPU sec, 2.808 real sec...
[M::process] read 672734 sequences (100000106 bp) in 1.490 CPU sec, 1.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283734, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (284.44, 150.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672988 reads in 199.017 CPU sec, 6.378 real sec
[M::mem_process_seqs] Processed 672692 reads in 113.138 CPU sec, 3.645 real sec
sorting block 44, #reads = 1079740 mem = 536880081 peak = 935124992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283885, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (288.52, 153.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672876 sequences (100000018 bp) in 2.804 CPU sec, 3.130 real sec...
[M::process] read 673106 sequences (100000189 bp) in 1.260 CPU sec, 1.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284190, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (286.60, 152.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672526 reads in 198.789 CPU sec, 6.371 real sec
sorting block 45, #reads = 1078848 mem = 536879044 peak = 935124992
[M::mem_process_seqs] Processed 672734 reads in 114.391 CPU sec, 3.696 real sec
sorting block 46, #reads = 1078941 mem = 536878223 peak = 935124992
[M::process] read 673502 sequences (100000045 bp) in 2.060 CPU sec, 2.299 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283734, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.27, 149.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 256, 1634)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4606)
[M::mem_pestat] mean and std.dev: (1197.55, 1591.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7563)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673290 sequences (100000272 bp) in 1.178 CPU sec, 1.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284674, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (275.41, 144.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 779, 1200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3550)
[M::mem_pestat] mean and std.dev: (664.62, 552.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4725)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672876 reads in 190.364 CPU sec, 6.235 real sec
[M::mem_process_seqs] Processed 673106 reads in 102.611 CPU sec, 3.312 real sec
sorting block 47, #reads = 1079430 mem = 536878738 peak = 935124992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285019, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.01, 136.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673266 sequences (100000234 bp) in 2.636 CPU sec, 2.923 real sec...
[M::process] read 673182 sequences (100000190 bp) in 1.400 CPU sec, 1.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284706, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.80, 140.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673502 reads in 184.268 CPU sec, 5.893 real sec
[M::mem_process_seqs] Processed 673290 reads in 102.103 CPU sec, 3.268 real sec
sorting block 48, #reads = 1080018 mem = 536880855 peak = 935124992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284734, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.76, 140.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673096 sequences (100000086 bp) in 2.588 CPU sec, 2.865 real sec...
[M::process] read 672960 sequences (100000124 bp) in 1.380 CPU sec, 1.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284836, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.69, 143.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673266 reads in 191.433 CPU sec, 6.122 real sec
[M::mem_process_seqs] Processed 673182 reads in 107.009 CPU sec, 3.427 real sec
sorting block 49, #reads = 1079233 mem = 536880436 peak = 935124992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284163, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.85, 145.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673312 sequences (100000118 bp) in 2.573 CPU sec, 2.839 real sec...
[M::process] read 673104 sequences (100000234 bp) in 1.560 CPU sec, 1.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284593, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (276.78, 149.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 211, 2663)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7889)
[M::mem_pestat] mean and std.dev: (1544.60, 2371.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11031)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673096 reads in 185.093 CPU sec, 5.909 real sec
[M::mem_process_seqs] Processed 672960 reads in 101.914 CPU sec, 3.261 real sec
sorting block 50, #reads = 1079795 mem = 536878207 peak = 935124992
sorting block 51, #reads = 1078915 mem = 536878690 peak = 935124992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284770, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.31, 140.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673268 sequences (100000228 bp) in 2.847 CPU sec, 3.083 real sec...
[M::process] read 673138 sequences (100000029 bp) in 1.620 CPU sec, 1.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284357, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.16, 141.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673312 reads in 182.790 CPU sec, 5.826 real sec
[M::mem_process_seqs] Processed 673104 reads in 103.489 CPU sec, 3.293 real sec
sorting block 52, #reads = 1079317 mem = 536880374 peak = 935124992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284667, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.75, 139.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 23, 165)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (47.85, 58.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673040 sequences (100000118 bp) in 3.283 CPU sec, 3.560 real sec...
[M::process] read 672834 sequences (100000009 bp) in 1.520 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285114, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (273.40, 140.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673268 reads in 183.894 CPU sec, 5.859 real sec
[M::mem_process_seqs] Processed 673138 reads in 102.351 CPU sec, 3.262 real sec
sorting block 53, #reads = 1079646 mem = 536881487 peak = 935124992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283991, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (273.01, 141.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672712 sequences (100000190 bp) in 2.399 CPU sec, 2.534 real sec...
[M::process] read 672790 sequences (100000069 bp) in 1.740 CPU sec, 1.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283500, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.54, 148.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673040 reads in 187.819 CPU sec, 5.992 real sec
[M::mem_process_seqs] Processed 672834 reads in 103.046 CPU sec, 3.285 real sec
sorting block 54, #reads = 1079515 mem = 536878324 peak = 935124992
[M::process] read 672902 sequences (100000261 bp) in 2.316 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284173, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (288.38, 154.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673050 sequences (100000284 bp) in 1.500 CPU sec, 1.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283371, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 206, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (287.04, 153.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 186, 186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480)
[M::mem_pestat] mean and std.dev: (125.83, 83.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 627)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283440, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.40, 149.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 102, 165)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (89.69, 71.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 621)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672712 reads in 285.045 CPU sec, 9.137 real sec
sorting block 55, #reads = 1079130 mem = 536878979 peak = 935124992
[M::mem_process_seqs] Processed 672790 reads in 197.643 CPU sec, 6.365 real sec
[M::mem_process_seqs] Processed 672902 reads in 205.315 CPU sec, 6.641 real sec
sorting block 56, #reads = 1079128 mem = 536879788 peak = 941113344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284168, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.96, 143.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673050 reads in 103.614 CPU sec, 3.429 real sec
[M::process] read 673596 sequences (100000041 bp) in 3.778 CPU sec, 4.234 real sec...
sorting block 57, #reads = 1079113 mem = 536881538 peak = 941113344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285141, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (256.94, 131.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673226 sequences (100000118 bp) in 2.792 CPU sec, 3.197 real sec...
[M::mem_process_seqs] Processed 673596 reads in 100.148 CPU sec, 3.265 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284934, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.11, 139.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 93, 1055)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3135)
[M::mem_pestat] mean and std.dev: (177.45, 288.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4175)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 58, #reads = 1080182 mem = 536880347 peak = 941113344
[M::process] read 673220 sequences (100000133 bp) in 2.783 CPU sec, 3.154 real sec...
[M::mem_process_seqs] Processed 673226 reads in 99.277 CPU sec, 3.214 real sec
[M::process] read 673118 sequences (100000008 bp) in 2.752 CPU sec, 3.059 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284270, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.52, 142.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673114 sequences (100000116 bp) in 1.290 CPU sec, 1.291 real sec...
[M::process] read 673100 sequences (100000190 bp) in 1.284 CPU sec, 1.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284281, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.13, 144.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673220 reads in 190.213 CPU sec, 6.020 real sec
[M::mem_process_seqs] Processed 673118 reads in 105.231 CPU sec, 3.342 real sec
sorting block 59, #reads = 1079776 mem = 536880673 peak = 941113344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284106, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.99, 146.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673332 sequences (100000157 bp) in 2.707 CPU sec, 2.966 real sec...
[M::process] read 673316 sequences (100000026 bp) in 1.380 CPU sec, 1.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284158, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.29, 148.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673114 reads in 189.816 CPU sec, 6.060 real sec
sorting block 60, #reads = 1079543 mem = 536878356 peak = 941113344
[M::mem_process_seqs] Processed 673100 reads in 101.867 CPU sec, 3.245 real sec
sorting block 61, #reads = 1079299 mem = 536877538 peak = 970543104
[M::process] read 673292 sequences (100000217 bp) in 1.802 CPU sec, 1.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285157, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.08, 137.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673414 sequences (100000195 bp) in 1.420 CPU sec, 1.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284541, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.03, 137.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (65, 173, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (194.31, 180.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284719, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.43, 139.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673332 reads in 266.451 CPU sec, 8.553 real sec
[M::mem_process_seqs] Processed 673292 reads in 187.142 CPU sec, 6.039 real sec
[M::mem_process_seqs] Processed 673316 reads in 194.456 CPU sec, 6.297 real sec
sorting block 62, #reads = 1079354 mem = 536880897 peak = 970543104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284461, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.27, 136.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (94, 384, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (277.06, 186.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673414 reads in 99.665 CPU sec, 3.319 real sec
[M::process] read 673428 sequences (100000145 bp) in 3.458 CPU sec, 3.928 real sec...
sorting block 63, #reads = 1079457 mem = 536880238 peak = 970543104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284180, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.56, 136.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 25, 180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (40.54, 60.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673428 reads in 115.239 CPU sec, 3.777 real sec
[M::process] read 673138 sequences (100000290 bp) in 3.434 CPU sec, 3.853 real sec...
sorting block 64, #reads = 1079334 mem = 536879372 peak = 970543104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284267, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.51, 141.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 217, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (150.00, 115.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673138 reads in 115.983 CPU sec, 3.768 real sec
[M::process] read 672858 sequences (100000028 bp) in 3.779 CPU sec, 4.271 real sec...
[M::process] read 672900 sequences (100000143 bp) in 2.562 CPU sec, 2.809 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283702, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.65, 147.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 33, 122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358)
[M::mem_pestat] mean and std.dev: (40.09, 46.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672832 sequences (100000138 bp) in 1.899 CPU sec, 1.900 real sec...
[M::process] read 673338 sequences (100000180 bp) in 1.889 CPU sec, 1.897 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283357, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.06, 149.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672858 reads in 235.877 CPU sec, 7.449 real sec
[M::mem_process_seqs] Processed 672900 reads in 131.097 CPU sec, 4.161 real sec
sorting block 65, #reads = 1079495 mem = 536877675 peak = 970543104
sorting block 66, #reads = 1079235 mem = 536879934 peak = 970543104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283931, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.01, 149.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 197, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (112.44, 102.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673720 sequences (100000122 bp) in 3.290 CPU sec, 3.636 real sec...
[M::process] read 673300 sequences (100000041 bp) in 2.002 CPU sec, 2.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284040, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.30, 140.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672832 reads in 235.848 CPU sec, 7.535 real sec
[M::mem_process_seqs] Processed 673338 reads in 128.107 CPU sec, 4.076 real sec
sorting block 67, #reads = 1079106 mem = 536878824 peak = 970543104
[M::process] read 673240 sequences (100000112 bp) in 2.331 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284996, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.04, 132.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 130, 943)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2763)
[M::mem_pestat] mean and std.dev: (202.86, 307.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3673)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673130 sequences (100000285 bp) in 1.350 CPU sec, 1.351 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284267, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.95, 142.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284588, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.77, 141.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 429, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207)
[M::mem_pestat] mean and std.dev: (230.64, 215.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1596)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673720 reads in 325.504 CPU sec, 10.466 real sec
[M::mem_process_seqs] Processed 673240 reads in 224.491 CPU sec, 7.251 real sec
[M::mem_process_seqs] Processed 673300 reads in 233.555 CPU sec, 7.572 real sec
sorting block 68, #reads = 1080175 mem = 536878892 peak = 970543104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284558, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.24, 144.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673130 reads in 117.236 CPU sec, 3.913 real sec
[M::process] read 673146 sequences (100000077 bp) in 3.709 CPU sec, 4.215 real sec...
sorting block 69, #reads = 1079641 mem = 536878255 peak = 970543104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285147, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (276.90, 150.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673146 reads in 116.415 CPU sec, 3.866 real sec
[M::process] read 673132 sequences (100000133 bp) in 3.505 CPU sec, 4.122 real sec...
sorting block 70, #reads = 1079755 mem = 536880206 peak = 970543104
[M::process] read 673702 sequences (100000139 bp) in 2.384 CPU sec, 2.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284982, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (276.57, 149.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673632 sequences (100000089 bp) in 1.190 CPU sec, 1.197 real sec...
[M::process] read 673672 sequences (100000263 bp) in 1.210 CPU sec, 1.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285641, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.45, 136.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673132 reads in 195.807 CPU sec, 6.215 real sec
[M::mem_process_seqs] Processed 673702 reads in 108.366 CPU sec, 3.455 real sec
sorting block 71, #reads = 1078820 mem = 536878638 peak = 970543104
[M::process] read 673492 sequences (100000277 bp) in 2.127 CPU sec, 2.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285066, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.26, 135.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673422 sequences (100000164 bp) in 1.220 CPU sec, 1.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285525, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.50, 131.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284990, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.85, 133.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 189, 1421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4213)
[M::mem_pestat] mean and std.dev: (503.91, 607.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5609)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673632 reads in 295.430 CPU sec, 9.495 real sec
[M::mem_process_seqs] Processed 673672 reads in 208.634 CPU sec, 6.733 real sec
sorting block 72, #reads = 1078993 mem = 536879902 peak = 970543104
[M::mem_process_seqs] Processed 673492 reads in 217.730 CPU sec, 7.058 real sec
sorting block 73, #reads = 1079715 mem = 536879258 peak = 970543104
[M::process] read 673080 sequences (100000049 bp) in 2.185 CPU sec, 2.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284721, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.99, 135.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673236 sequences (100000227 bp) in 2.273 CPU sec, 2.503 real sec...
[M::process] read 673050 sequences (100000077 bp) in 1.670 CPU sec, 1.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284248, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.06, 139.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673422 reads in 222.262 CPU sec, 7.117 real sec
[M::mem_process_seqs] Processed 673080 reads in 121.096 CPU sec, 3.856 real sec
sorting block 74, #reads = 1078788 mem = 536878168 peak = 970543104
[M::process] read 673068 sequences (100000247 bp) in 1.849 CPU sec, 1.997 real sec...
[M::process] read 673474 sequences (100000022 bp) in 1.503 CPU sec, 1.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283756, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (274.50, 143.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 668, 687)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1827)
[M::mem_pestat] mean and std.dev: (474.22, 306.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2397)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673050 reads in 116.885 CPU sec, 3.768 real sec
sorting block 75, #reads = 1079598 mem = 536880382 peak = 970543104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284016, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (274.01, 143.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 47, 65)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 167)
[M::mem_pestat] mean and std.dev: (36.67, 26.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 218)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673472 sequences (100000129 bp) in 1.344 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283771, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.40, 144.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673236 reads in 343.072 CPU sec, 11.024 real sec
[M::mem_process_seqs] Processed 673068 reads in 239.775 CPU sec, 7.733 real sec
sorting block 76, #reads = 1078950 mem = 536877016 peak = 970543104
[M::process] read 673276 sequences (100000190 bp) in 2.066 CPU sec, 2.263 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285067, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.69, 136.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673088 sequences (100000169 bp) in 1.150 CPU sec, 1.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285430, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.54, 134.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 643, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1903)
[M::mem_pestat] mean and std.dev: (517.00, 542.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2687)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284588, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.99, 141.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 20, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239)
[M::mem_pestat] mean and std.dev: (35.92, 41.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 315)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673474 reads in 277.030 CPU sec, 8.907 real sec
[M::mem_process_seqs] Processed 673472 reads in 189.145 CPU sec, 6.107 real sec
sorting block 77, #reads = 1079308 mem = 536876914 peak = 970543104
[M::mem_process_seqs] Processed 673276 reads in 197.095 CPU sec, 6.395 real sec
sorting block 78, #reads = 1079381 mem = 536880624 peak = 970543104
[M::process] read 672934 sequences (100000168 bp) in 2.184 CPU sec, 2.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283944, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.14, 143.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 365, 894)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2592)
[M::mem_pestat] mean and std.dev: (631.50, 702.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3441)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672918 sequences (100000062 bp) in 2.071 CPU sec, 2.144 real sec...
[M::process] read 673018 sequences (100000002 bp) in 1.590 CPU sec, 1.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284522, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (276.00, 146.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 132, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2148)
[M::mem_pestat] mean and std.dev: (343.00, 323.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2835)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673088 reads in 209.486 CPU sec, 6.686 real sec
[M::mem_process_seqs] Processed 672934 reads in 117.819 CPU sec, 3.743 real sec
sorting block 79, #reads = 1079612 mem = 536879430 peak = 970543104
[M::process] read 96244 sequences (14300550 bp) in 0.522 CPU sec, 0.565 real sec...
sorting block 80, #reads = 1079618 mem = 536879673 peak = 970543104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284279, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (280.50, 151.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 193, 193)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (94.30, 83.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 697)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 40604, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (276.42, 150.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283655, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (280.26, 152.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672918 reads in 223.338 CPU sec, 7.245 real sec
[M::mem_process_seqs] Processed 96244 reads in 123.705 CPU sec, 4.009 real sec
[M::mem_process_seqs] Processed 673018 reads in 132.498 CPU sec, 4.328 real sec
sorting block 81, #reads = 1079383 mem = 536880785 peak = 970543104
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 88268338
overall: 69550211072 mem 15054.570 user 746.617 sys 529.873 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L004\tPU:L004\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.T18-D0003255_R1.fastq.gz 0002.T18-D0003255_R2.fastq.gz
[main] Real time: 529.903 sec; CPU: 15801.190 sec
sorting block 82, #reads = 375398 mem = 186724270 peak = 970543104
uniq header count 1
merging 83 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 3369988227
output reads: 88900077
bam_mem_sort: 83 calls 47.334 user 0.670 sys 48.116 real
bam_write: 83 calls 25.245 user 1.290 sys 26.594 real
execute: 1 calls 26.850 user 1.739 sys 559.723 real
merge_files: 1 calls 26.835 user 1.713 sys 29.505 real
parse_chunk: 1318 calls 53.952 user 14.124 sys 69.016 real
read_chunk: 3547 calls 40.048 user 28.715 sys 464.819 real
sort_block: 1 calls 29.765 user 27.728 sys 448.753 real
write_chunk: 2227 calls 26.443 user 1.411 sys 28.209 real
overall: 2382344192 mem 946.865 user 65.552 sys 559.741 real
ls: cannot access '*.cram': No such file or directory