.command.log - Workdir - DAQ:SENTIEON_BWAMEM (T18-D0003255;L001;0004)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/dd/410e437bed828c22c5d39583e62c91/.command.log
Size: 122.6 KB
Task: DAQ:SENTIEON_BWAMEM (T18-D0003255;L001;0004)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/dd/410e437bed828c22c5d39583e62c91/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/97/7870e9779bacdde37039d765313caa/T18-D0003255/0004.T18-D0003255_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/97/7870e9779bacdde37039d765313caa/T18-D0003255/0004.T18-D0003255_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/dd/410e437bed828c22c5d39583e62c91/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 2296.747 CPU sec, 496.858 real sec
[M::process] read 673490 sequences (100000292 bp) in 1.316 CPU sec, 1.317 real sec...
[M::process] read 673464 sequences (100000186 bp) in 1.751 CPU sec, 1.797 real sec...
[M::process] read 673260 sequences (100000122 bp) in 1.676 CPU sec, 1.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285942, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (260.83, 129.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673344 sequences (100000168 bp) in 1.497 CPU sec, 1.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286246, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (266.60, 134.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286376, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.70, 132.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673490 reads in 349.648 CPU sec, 11.322 real sec
[M::mem_process_seqs] Processed 673260 reads in 212.762 CPU sec, 6.945 real sec
[M::mem_process_seqs] Processed 673464 reads in 222.986 CPU sec, 7.311 real sec
sorting block 0, #reads = 1079498 mem = 536878252 peak = 593084416
[M::process] read 673168 sequences (100000201 bp) in 2.370 CPU sec, 2.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285726, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (266.02, 133.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 196, 3846)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11364)
[M::mem_pestat] mean and std.dev: (1045.31, 1549.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15123)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673342 sequences (100000094 bp) in 1.659 CPU sec, 1.722 real sec...
[M::process] read 673470 sequences (100000008 bp) in 1.457 CPU sec, 1.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285366, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.20, 137.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673344 reads in 216.277 CPU sec, 7.023 real sec
sorting block 1, #reads = 1079658 mem = 536879160 peak = 750370816
[M::mem_process_seqs] Processed 673168 reads in 123.871 CPU sec, 4.081 real sec
sorting block 2, #reads = 1079536 mem = 536879504 peak = 855027712
[M::process] read 673304 sequences (100000015 bp) in 2.689 CPU sec, 2.892 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285346, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.69, 138.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673256 sequences (100000230 bp) in 1.100 CPU sec, 1.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284977, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.38, 136.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 284, 1091)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3247)
[M::mem_pestat] mean and std.dev: (392.50, 469.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4325)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285257, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.62, 136.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673342 reads in 318.834 CPU sec, 10.269 real sec
[M::mem_process_seqs] Processed 673470 reads in 221.258 CPU sec, 7.202 real sec
[M::mem_process_seqs] Processed 673304 reads in 233.768 CPU sec, 7.653 real sec
sorting block 3, #reads = 1079961 mem = 536879730 peak = 855027712
[M::process] read 673078 sequences (100000152 bp) in 2.274 CPU sec, 2.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284508, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.93, 137.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1079970 mem = 536879242 peak = 855027712
[M::process] read 673180 sequences (100000105 bp) in 2.204 CPU sec, 2.376 real sec...
[M::process] read 673042 sequences (100000166 bp) in 1.470 CPU sec, 1.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284711, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.32, 139.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673256 reads in 245.471 CPU sec, 7.904 real sec
[M::mem_process_seqs] Processed 673078 reads in 139.880 CPU sec, 4.515 real sec
sorting block 5, #reads = 1078994 mem = 536880023 peak = 855027712
[M::process] read 673078 sequences (100000193 bp) in 2.442 CPU sec, 2.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284329, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.96, 140.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672906 sequences (100000138 bp) in 1.350 CPU sec, 1.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284676, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.74, 142.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 185, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2180)
[M::mem_pestat] mean and std.dev: (367.27, 423.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2904)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284679, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (275.84, 144.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 211, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (197.00, 140.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673180 reads in 329.905 CPU sec, 10.640 real sec
[M::mem_process_seqs] Processed 673042 reads in 236.262 CPU sec, 7.669 real sec
[M::mem_process_seqs] Processed 673078 reads in 249.054 CPU sec, 8.147 real sec
sorting block 6, #reads = 1078355 mem = 536879282 peak = 855027712
[M::process] read 672698 sequences (100000154 bp) in 2.774 CPU sec, 2.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284595, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.71, 145.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 87, 719)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2127)
[M::mem_pestat] mean and std.dev: (228.81, 288.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2831)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 7, #reads = 1079239 mem = 536879062 peak = 855027712
[M::process] read 672764 sequences (100000158 bp) in 1.876 CPU sec, 2.043 real sec...
[M::process] read 673378 sequences (100000217 bp) in 1.100 CPU sec, 1.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284753, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.07, 148.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672906 reads in 241.693 CPU sec, 7.810 real sec
[M::mem_process_seqs] Processed 672698 reads in 138.901 CPU sec, 4.500 real sec
sorting block 8, #reads = 1079564 mem = 536878757 peak = 855027712
[M::process] read 673322 sequences (100000105 bp) in 2.536 CPU sec, 2.840 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284489, 15, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.40, 149.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 102, 178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474)
[M::mem_pestat] mean and std.dev: (89.08, 73.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 622)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673326 sequences (100000241 bp) in 1.140 CPU sec, 1.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286299, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.98, 134.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (65, 96, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361)
[M::mem_pestat] mean and std.dev: (204.67, 225.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1793)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672764 reads in 213.792 CPU sec, 6.889 real sec
[M::mem_process_seqs] Processed 673378 reads in 115.331 CPU sec, 3.747 real sec
sorting block 9, #reads = 1079410 mem = 536880705 peak = 855027712
[M::process] read 673142 sequences (100000219 bp) in 1.863 CPU sec, 2.132 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286596, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.53, 134.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673082 sequences (100000160 bp) in 1.040 CPU sec, 1.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285464, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.85, 137.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285816, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.29, 138.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673322 reads in 299.030 CPU sec, 9.684 real sec
[M::mem_process_seqs] Processed 673326 reads in 213.252 CPU sec, 6.965 real sec
sorting block 10, #reads = 1080321 mem = 536882579 peak = 855027712
[M::mem_process_seqs] Processed 673142 reads in 225.055 CPU sec, 7.394 real sec
sorting block 11, #reads = 1080152 mem = 536879536 peak = 855027712
[M::process] read 673062 sequences (100000120 bp) in 2.242 CPU sec, 2.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285708, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.44, 141.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 44, 185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (77.83, 76.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673372 sequences (100000130 bp) in 2.359 CPU sec, 2.625 real sec...
[M::process] read 673200 sequences (100000102 bp) in 1.490 CPU sec, 1.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285354, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.55, 144.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673082 reads in 222.405 CPU sec, 7.158 real sec
[M::mem_process_seqs] Processed 673062 reads in 132.506 CPU sec, 4.269 real sec
sorting block 12, #reads = 1079798 mem = 536880993 peak = 855027712
[M::process] read 673168 sequences (100000181 bp) in 2.000 CPU sec, 2.154 real sec...
[M::process] read 673018 sequences (100000017 bp) in 1.150 CPU sec, 1.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285101, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.51, 140.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (350, 1183, 1183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2849)
[M::mem_pestat] mean and std.dev: (863.73, 564.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3682)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673168 reads in 113.656 CPU sec, 3.690 real sec
sorting block 13, #reads = 1079697 mem = 536881565 peak = 855027712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285609, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.05, 137.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 47, 595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1701)
[M::mem_pestat] mean and std.dev: (223.83, 288.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2254)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673156 sequences (100000234 bp) in 1.779 CPU sec, 1.781 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285121, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.99, 137.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673372 reads in 330.765 CPU sec, 10.660 real sec
[M::mem_process_seqs] Processed 673200 reads in 240.111 CPU sec, 7.790 real sec
sorting block 14, #reads = 1079646 mem = 536879502 peak = 855027712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285052, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.09, 142.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673094 sequences (100000278 bp) in 2.263 CPU sec, 2.382 real sec...
[M::process] read 672910 sequences (100000089 bp) in 1.740 CPU sec, 1.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285513, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.99, 142.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (57, 219, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (165.08, 124.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673018 reads in 210.907 CPU sec, 6.784 real sec
[M::mem_process_seqs] Processed 673156 reads in 123.009 CPU sec, 3.985 real sec
sorting block 15, #reads = 1079776 mem = 536879089 peak = 855027712
[M::process] read 672828 sequences (100000230 bp) in 2.374 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285128, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (276.27, 145.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672606 sequences (100000237 bp) in 1.270 CPU sec, 1.269 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284527, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.57, 147.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284911, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.24, 145.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 54, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (128.23, 160.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 885)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673094 reads in 312.943 CPU sec, 10.093 real sec
sorting block 16, #reads = 1079942 mem = 536878356 peak = 855027712
[M::mem_process_seqs] Processed 672828 reads in 222.967 CPU sec, 7.242 real sec
[M::mem_process_seqs] Processed 672910 reads in 235.628 CPU sec, 7.697 real sec
sorting block 17, #reads = 1079003 mem = 536879337 peak = 855027712
[M::process] read 672512 sequences (100000083 bp) in 2.397 CPU sec, 2.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284696, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (283.14, 148.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 130, 1473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4373)
[M::mem_pestat] mean and std.dev: (577.17, 671.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5823)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672904 sequences (100000060 bp) in 1.570 CPU sec, 1.728 real sec...
[M::process] read 673258 sequences (100000166 bp) in 1.090 CPU sec, 1.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284923, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (285.92, 149.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 210, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2126)
[M::mem_pestat] mean and std.dev: (409.29, 483.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2827)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672606 reads in 212.114 CPU sec, 6.871 real sec
sorting block 18, #reads = 1079182 mem = 536877622 peak = 855027712
[M::mem_process_seqs] Processed 672512 reads in 123.098 CPU sec, 3.989 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286051, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.87, 144.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 359, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (262.33, 140.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673212 sequences (100000296 bp) in 2.563 CPU sec, 2.914 real sec...
sorting block 19, #reads = 1079247 mem = 536877861 peak = 855027712
[M::process] read 673056 sequences (100000283 bp) in 1.076 CPU sec, 1.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286923, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.16, 133.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672904 reads in 193.160 CPU sec, 6.214 real sec
[M::mem_process_seqs] Processed 673258 reads in 114.294 CPU sec, 3.712 real sec
sorting block 20, #reads = 1079540 mem = 536878938 peak = 855027712
[M::process] read 672986 sequences (100000137 bp) in 1.971 CPU sec, 2.047 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286402, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.86, 138.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 122, 185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (93.00, 76.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 665)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672924 sequences (100000032 bp) in 1.270 CPU sec, 1.264 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286008, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.06, 138.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 199, 8685)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25873)
[M::mem_pestat] mean and std.dev: (2890.91, 3666.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 34467)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285547, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.39, 139.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673212 reads in 276.290 CPU sec, 8.899 real sec
[M::mem_process_seqs] Processed 673056 reads in 200.020 CPU sec, 6.485 real sec
[M::mem_process_seqs] Processed 672986 reads in 212.884 CPU sec, 6.948 real sec
sorting block 21, #reads = 1080102 mem = 536880280 peak = 855027712
[M::process] read 673178 sequences (100000083 bp) in 1.737 CPU sec, 1.880 real sec...
sorting block 22, #reads = 1079318 mem = 536881079 peak = 869363712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285545, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.58, 144.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 20, 94)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256)
[M::mem_pestat] mean and std.dev: (68.00, 77.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 376)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673404 sequences (100000128 bp) in 1.873 CPU sec, 1.958 real sec...
[M::process] read 673176 sequences (100000092 bp) in 1.110 CPU sec, 1.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285497, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.87, 142.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (424, 703, 999)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2149)
[M::mem_pestat] mean and std.dev: (761.09, 579.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3079)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672924 reads in 199.485 CPU sec, 6.403 real sec
[M::mem_process_seqs] Processed 673178 reads in 116.363 CPU sec, 3.757 real sec
sorting block 23, #reads = 1079769 mem = 536880237 peak = 869363712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286019, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.09, 136.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 28, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568)
[M::mem_pestat] mean and std.dev: (47.55, 55.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673028 sequences (100000065 bp) in 2.859 CPU sec, 3.161 real sec...
[M::process] read 673148 sequences (100000031 bp) in 1.075 CPU sec, 1.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285816, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.98, 140.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673404 reads in 199.204 CPU sec, 6.398 real sec
[M::mem_process_seqs] Processed 673176 reads in 117.104 CPU sec, 3.784 real sec
sorting block 24, #reads = 1080338 mem = 536878388 peak = 869363712
[M::process] read 673056 sequences (100000252 bp) in 1.894 CPU sec, 2.024 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285352, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.21, 142.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 130, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416)
[M::mem_pestat] mean and std.dev: (143.92, 100.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 547)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673048 sequences (100000260 bp) in 1.190 CPU sec, 1.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285762, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (275.65, 143.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285589, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.31, 144.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 223, 731)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2145)
[M::mem_pestat] mean and std.dev: (309.27, 333.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2852)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673028 reads in 283.636 CPU sec, 9.131 real sec
[M::mem_process_seqs] Processed 673148 reads in 200.181 CPU sec, 6.509 real sec
sorting block 25, #reads = 1079803 mem = 536881410 peak = 869363712
[M::mem_process_seqs] Processed 673056 reads in 213.206 CPU sec, 6.981 real sec
sorting block 26, #reads = 1079970 mem = 536877707 peak = 869363712
[M::process] read 672818 sequences (100000133 bp) in 1.927 CPU sec, 2.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283009, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (275.62, 144.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 98, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (142.67, 188.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672824 sequences (100000026 bp) in 2.066 CPU sec, 2.289 real sec...
[M::process] read 672634 sequences (100000214 bp) in 1.130 CPU sec, 1.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284957, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.91, 149.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 661, 703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2075)
[M::mem_pestat] mean and std.dev: (317.22, 332.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2761)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673048 reads in 202.735 CPU sec, 6.494 real sec
[M::mem_process_seqs] Processed 672818 reads in 121.891 CPU sec, 3.925 real sec
sorting block 27, #reads = 1075878 mem = 536879983 peak = 869363712
[M::process] read 672816 sequences (100000226 bp) in 2.129 CPU sec, 2.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284834, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.40, 148.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 403, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2108)
[M::mem_pestat] mean and std.dev: (369.23, 281.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2796)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673356 sequences (100000096 bp) in 1.150 CPU sec, 1.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284512, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (285.29, 149.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285548, 14, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.22, 145.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 60, 1374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4086)
[M::mem_pestat] mean and std.dev: (461.23, 695.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5442)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672824 reads in 289.281 CPU sec, 9.289 real sec
sorting block 28, #reads = 1079599 mem = 536876978 peak = 869429248
[M::mem_process_seqs] Processed 672634 reads in 201.517 CPU sec, 6.519 real sec
[M::mem_process_seqs] Processed 672816 reads in 211.185 CPU sec, 6.868 real sec
sorting block 29, #reads = 1079475 mem = 536879881 peak = 935223296
[M::process] read 673070 sequences (100000037 bp) in 1.976 CPU sec, 2.165 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286400, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.28, 132.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673234 sequences (100000285 bp) in 1.599 CPU sec, 1.702 real sec...
[M::process] read 673012 sequences (100000265 bp) in 1.130 CPU sec, 1.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285582, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (268.22, 135.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673356 reads in 195.714 CPU sec, 6.268 real sec
[M::mem_process_seqs] Processed 673070 reads in 110.168 CPU sec, 3.544 real sec
sorting block 30, #reads = 1079002 mem = 536879501 peak = 935223296
sorting block 31, #reads = 1080084 mem = 536879608 peak = 935223296
[M::process] read 672934 sequences (100000233 bp) in 2.119 CPU sec, 2.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285998, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.64, 137.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 52, 180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (81.25, 90.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672918 sequences (100000245 bp) in 1.160 CPU sec, 1.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285845, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.32, 138.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284773, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (275.03, 140.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673234 reads in 276.557 CPU sec, 8.895 real sec
[M::mem_process_seqs] Processed 673012 reads in 197.150 CPU sec, 6.388 real sec
[M::mem_process_seqs] Processed 672934 reads in 210.465 CPU sec, 6.869 real sec
sorting block 32, #reads = 1079996 mem = 536878701 peak = 935223296
[M::process] read 673368 sequences (100000101 bp) in 1.907 CPU sec, 2.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284920, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.12, 143.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (248, 1131, 3845)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11039)
[M::mem_pestat] mean and std.dev: (1766.50, 2150.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14636)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673108 sequences (100000135 bp) in 2.105 CPU sec, 2.313 real sec...
[M::process] read 673122 sequences (100000106 bp) in 1.160 CPU sec, 1.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285013, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.75, 133.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672918 reads in 196.540 CPU sec, 6.297 real sec
sorting block 33, #reads = 1079857 mem = 536878433 peak = 935223296
[M::mem_process_seqs] Processed 673368 reads in 113.712 CPU sec, 3.656 real sec
sorting block 34, #reads = 1079808 mem = 536879524 peak = 935223296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285153, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.71, 137.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 62)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 180)
[M::mem_pestat] mean and std.dev: (30.09, 32.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 239)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672890 sequences (100000018 bp) in 2.291 CPU sec, 2.574 real sec...
[M::process] read 672828 sequences (100000091 bp) in 1.140 CPU sec, 1.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284985, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.72, 139.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 962, 4381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13111)
[M::mem_pestat] mean and std.dev: (2376.10, 3397.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17476)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673108 reads in 190.358 CPU sec, 6.114 real sec
[M::mem_process_seqs] Processed 673122 reads in 112.002 CPU sec, 3.637 real sec
sorting block 35, #reads = 1079386 mem = 536879388 peak = 935223296
[M::process] read 672976 sequences (100000057 bp) in 1.929 CPU sec, 2.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285057, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.65, 140.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672898 sequences (100000107 bp) in 1.140 CPU sec, 1.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284771, 17, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.75, 144.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 91, 983)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2889)
[M::mem_pestat] mean and std.dev: (391.53, 447.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3842)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284746, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.93, 142.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 79, 88)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172)
[M::mem_pestat] mean and std.dev: (61.78, 21.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 214)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672890 reads in 275.950 CPU sec, 8.878 real sec
[M::mem_process_seqs] Processed 672976 reads in 199.390 CPU sec, 6.471 real sec
[M::mem_process_seqs] Processed 672828 reads in 211.088 CPU sec, 6.894 real sec
sorting block 36, #reads = 1079486 mem = 536879171 peak = 935223296
[M::process] read 672912 sequences (100000003 bp) in 2.523 CPU sec, 2.845 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284880, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.71, 144.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 37, #reads = 1079890 mem = 536878478 peak = 935223296
[M::process] read 672654 sequences (100000146 bp) in 1.725 CPU sec, 1.861 real sec...
[M::process] read 672522 sequences (100000149 bp) in 1.130 CPU sec, 1.132 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285349, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.96, 147.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (37, 102, 1422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4192)
[M::mem_pestat] mean and std.dev: (900.83, 1164.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5577)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672898 reads in 196.032 CPU sec, 6.310 real sec
[M::mem_process_seqs] Processed 672912 reads in 114.376 CPU sec, 3.687 real sec
sorting block 38, #reads = 1079369 mem = 536879703 peak = 935223296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285435, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (281.92, 146.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 71, 691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2007)
[M::mem_pestat] mean and std.dev: (109.90, 195.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2665)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673498 sequences (100000197 bp) in 2.456 CPU sec, 2.770 real sec...
[M::process] read 673632 sequences (100000132 bp) in 1.120 CPU sec, 1.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284894, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (285.65, 149.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 208, 754)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2224)
[M::mem_pestat] mean and std.dev: (389.00, 476.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2959)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672654 reads in 181.656 CPU sec, 5.824 real sec
[M::mem_process_seqs] Processed 672522 reads in 106.668 CPU sec, 3.450 real sec
sorting block 39, #reads = 1079767 mem = 536877728 peak = 935223296
[M::process] read 673472 sequences (100000094 bp) in 1.687 CPU sec, 1.795 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284701, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (267.91, 141.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 33, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (55.18, 59.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673400 sequences (100000055 bp) in 1.090 CPU sec, 1.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284942, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.95, 140.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285062, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.44, 139.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673498 reads in 271.786 CPU sec, 8.751 real sec
[M::mem_process_seqs] Processed 673632 reads in 192.800 CPU sec, 6.254 real sec
sorting block 40, #reads = 1079026 mem = 536879207 peak = 935223296
[M::mem_process_seqs] Processed 673472 reads in 202.897 CPU sec, 6.620 real sec
sorting block 41, #reads = 1079803 mem = 536877655 peak = 935223296
[M::process] read 673354 sequences (100000073 bp) in 1.802 CPU sec, 1.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284731, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.71, 141.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673412 sequences (100000015 bp) in 2.124 CPU sec, 2.360 real sec...
[M::process] read 673554 sequences (100000080 bp) in 1.340 CPU sec, 1.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284508, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.13, 141.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673400 reads in 190.680 CPU sec, 6.107 real sec
[M::mem_process_seqs] Processed 673354 reads in 110.698 CPU sec, 3.551 real sec
sorting block 42, #reads = 1079686 mem = 536879464 peak = 935223296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284787, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.84, 137.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673354 sequences (100000277 bp) in 2.264 CPU sec, 2.543 real sec...
[M::process] read 673408 sequences (100000233 bp) in 1.110 CPU sec, 1.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284476, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.04, 134.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 213, 1788)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4938)
[M::mem_pestat] mean and std.dev: (661.27, 704.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6513)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673412 reads in 186.572 CPU sec, 5.979 real sec
sorting block 43, #reads = 1079624 mem = 536878560 peak = 935223296
[M::mem_process_seqs] Processed 673554 reads in 106.073 CPU sec, 3.429 real sec
sorting block 44, #reads = 1079667 mem = 536878469 peak = 935485440
[M::process] read 673372 sequences (100000250 bp) in 1.622 CPU sec, 1.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285132, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.38, 135.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673492 sequences (100000037 bp) in 1.055 CPU sec, 1.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284294, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.77, 138.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 292, 1502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4448)
[M::mem_pestat] mean and std.dev: (641.79, 649.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5921)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284273, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.43, 137.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 193, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (201.92, 245.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1449)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673354 reads in 272.446 CPU sec, 8.772 real sec
[M::mem_process_seqs] Processed 673372 reads in 192.013 CPU sec, 6.210 real sec
[M::mem_process_seqs] Processed 673408 reads in 200.922 CPU sec, 6.532 real sec
sorting block 45, #reads = 1079726 mem = 536878051 peak = 935485440
[M::process] read 673184 sequences (100000077 bp) in 2.543 CPU sec, 2.876 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284479, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.54, 134.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1079670 mem = 536877695 peak = 935485440
[M::process] read 673146 sequences (100000015 bp) in 1.564 CPU sec, 1.662 real sec...
[M::process] read 673132 sequences (100000281 bp) in 1.110 CPU sec, 1.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284114, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.36, 140.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673492 reads in 194.787 CPU sec, 6.255 real sec
[M::mem_process_seqs] Processed 673184 reads in 111.813 CPU sec, 3.580 real sec
sorting block 47, #reads = 1079683 mem = 536879328 peak = 935485440
[M::process] read 672826 sequences (100000005 bp) in 2.360 CPU sec, 2.637 real sec...
[M::process] read 672916 sequences (100000116 bp) in 1.160 CPU sec, 1.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283769, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (275.86, 145.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 86, 773)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2227)
[M::mem_pestat] mean and std.dev: (290.94, 420.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2954)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673132 reads in 98.256 CPU sec, 3.135 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284567, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.25, 142.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 68, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1660)
[M::mem_pestat] mean and std.dev: (125.36, 205.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2212)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673634 sequences (100000059 bp) in 1.085 CPU sec, 1.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284461, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (279.55, 148.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673146 reads in 282.889 CPU sec, 9.075 real sec
sorting block 48, #reads = 1079531 mem = 536877124 peak = 935485440
[M::mem_process_seqs] Processed 672826 reads in 112.622 CPU sec, 3.657 real sec
sorting block 49, #reads = 1078936 mem = 536878383 peak = 935485440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284220, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (280.74, 151.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673528 sequences (100000191 bp) in 2.442 CPU sec, 2.730 real sec...
[M::process] read 673422 sequences (100000096 bp) in 1.100 CPU sec, 1.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285352, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.37, 139.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672916 reads in 181.809 CPU sec, 5.823 real sec
[M::mem_process_seqs] Processed 673634 reads in 99.808 CPU sec, 3.206 real sec
sorting block 50, #reads = 1079235 mem = 536877570 peak = 935485440
[M::process] read 673266 sequences (100000042 bp) in 1.878 CPU sec, 2.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285317, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.12, 139.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 169, 689)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1975)
[M::mem_pestat] mean and std.dev: (282.11, 377.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2618)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673284 sequences (100000074 bp) in 1.100 CPU sec, 1.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285024, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.64, 143.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286033, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.37, 141.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673528 reads in 253.193 CPU sec, 8.132 real sec
[M::mem_process_seqs] Processed 673266 reads in 175.984 CPU sec, 5.677 real sec
[M::mem_process_seqs] Processed 673422 reads in 183.408 CPU sec, 5.941 real sec
sorting block 51, #reads = 1079696 mem = 536878687 peak = 935485440
[M::process] read 673326 sequences (100000112 bp) in 1.918 CPU sec, 2.077 real sec...
sorting block 52, #reads = 1079797 mem = 536878757 peak = 935485440
[M::process] read 673616 sequences (100000209 bp) in 1.876 CPU sec, 2.072 real sec...
[M::process] read 673450 sequences (100000212 bp) in 1.130 CPU sec, 1.132 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284679, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.20, 141.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 273, 5470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16368)
[M::mem_pestat] mean and std.dev: (1600.19, 2619.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21817)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673326 reads in 97.468 CPU sec, 3.122 real sec
sorting block 53, #reads = 1079806 mem = 536877412 peak = 935485440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284598, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.48, 145.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673478 sequences (100000288 bp) in 1.133 CPU sec, 1.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284692, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.84, 136.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673284 reads in 274.797 CPU sec, 8.840 real sec
[M::mem_process_seqs] Processed 673616 reads in 110.678 CPU sec, 3.592 real sec
sorting block 54, #reads = 1079705 mem = 536877715 peak = 935485440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284567, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.70, 138.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 23, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354)
[M::mem_pestat] mean and std.dev: (37.00, 39.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 466)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673324 sequences (100000170 bp) in 2.425 CPU sec, 2.722 real sec...
[M::process] read 673288 sequences (100000008 bp) in 1.200 CPU sec, 1.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284875, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (265.77, 138.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 24, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (141.70, 205.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673450 reads in 178.233 CPU sec, 5.692 real sec
[M::mem_process_seqs] Processed 673478 reads in 98.388 CPU sec, 3.145 real sec
sorting block 55, #reads = 1079590 mem = 536879357 peak = 935485440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283740, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.72, 139.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (37, 88, 88)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190)
[M::mem_pestat] mean and std.dev: (54.00, 33.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 241)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673344 sequences (100000159 bp) in 2.195 CPU sec, 2.461 real sec...
[M::process] read 673132 sequences (100000264 bp) in 1.100 CPU sec, 1.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281931, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (268.14, 141.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673324 reads in 171.807 CPU sec, 5.494 real sec
[M::mem_process_seqs] Processed 673288 reads in 95.493 CPU sec, 3.068 real sec
sorting block 56, #reads = 1079711 mem = 536879631 peak = 935485440
[M::process] read 673088 sequences (100000249 bp) in 1.682 CPU sec, 1.869 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285001, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.36, 143.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672928 sequences (100000231 bp) in 1.080 CPU sec, 1.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284296, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.92, 145.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284117, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (277.70, 149.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 130, 1804)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5346)
[M::mem_pestat] mean and std.dev: (661.46, 806.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7117)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673344 reads in 265.128 CPU sec, 8.526 real sec
[M::mem_process_seqs] Processed 673132 reads in 186.493 CPU sec, 6.024 real sec
sorting block 57, #reads = 1074643 mem = 536881488 peak = 935485440
[M::mem_process_seqs] Processed 673088 reads in 194.911 CPU sec, 6.327 real sec
sorting block 58, #reads = 1079836 mem = 536879037 peak = 989982720
[M::process] read 673314 sequences (100000262 bp) in 2.542 CPU sec, 2.853 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284581, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (280.96, 152.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 86, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (68.91, 67.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673446 sequences (100000081 bp) in 1.595 CPU sec, 1.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285002, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (272.66, 145.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673410 sequences (100000165 bp) in 1.070 CPU sec, 1.070 real sec...
[M::mem_process_seqs] Processed 672928 reads in 179.078 CPU sec, 5.738 real sec
[M::mem_process_seqs] Processed 673314 reads in 98.089 CPU sec, 3.122 real sec
sorting block 59, #reads = 1079365 mem = 536879083 peak = 989982720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285900, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (269.32, 140.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673232 sequences (100000248 bp) in 2.495 CPU sec, 2.856 real sec...
[M::process] read 673204 sequences (100000270 bp) in 1.080 CPU sec, 1.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285101, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (271.76, 142.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673446 reads in 171.768 CPU sec, 5.507 real sec
sorting block 60, #reads = 1079164 mem = 536878687 peak = 989982720
[M::mem_process_seqs] Processed 673410 reads in 97.067 CPU sec, 3.108 real sec
sorting block 61, #reads = 1079912 mem = 536878381 peak = 994734080
[M::process] read 673054 sequences (100000243 bp) in 1.753 CPU sec, 1.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285890, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.40, 144.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673208 sequences (100000247 bp) in 1.090 CPU sec, 1.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284622, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (275.26, 145.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284821, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (277.92, 148.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 72, 891)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2641)
[M::mem_pestat] mean and std.dev: (195.45, 329.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3516)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673232 reads in 251.060 CPU sec, 8.069 real sec
[M::mem_process_seqs] Processed 673204 reads in 176.809 CPU sec, 5.708 real sec
[M::mem_process_seqs] Processed 673054 reads in 184.919 CPU sec, 5.996 real sec
sorting block 62, #reads = 1079963 mem = 536879245 peak = 994734080
[M::process] read 673410 sequences (100000175 bp) in 2.356 CPU sec, 2.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285431, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.39, 137.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 79, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (135.80, 143.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 63, #reads = 1079306 mem = 536879718 peak = 994734080
[M::process] read 673356 sequences (100000217 bp) in 1.733 CPU sec, 1.845 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285597, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (262.92, 134.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673226 sequences (100000250 bp) in 1.100 CPU sec, 1.098 real sec...
[M::mem_process_seqs] Processed 673208 reads in 176.410 CPU sec, 5.650 real sec
[M::mem_process_seqs] Processed 673410 reads in 102.529 CPU sec, 3.278 real sec
sorting block 64, #reads = 1079510 mem = 536876979 peak = 994734080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284582, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (266.11, 139.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 87, 128)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334)
[M::mem_pestat] mean and std.dev: (57.88, 40.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 437)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673248 sequences (100000249 bp) in 2.310 CPU sec, 2.615 real sec...
[M::process] read 673154 sequences (100000244 bp) in 1.110 CPU sec, 1.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284768, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (267.31, 140.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 102, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (163.56, 212.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673356 reads in 183.164 CPU sec, 5.883 real sec
[M::mem_process_seqs] Processed 673226 reads in 104.894 CPU sec, 3.395 real sec
sorting block 65, #reads = 1079718 mem = 536880174 peak = 994734080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284985, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (268.45, 141.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673186 sequences (100000245 bp) in 2.060 CPU sec, 2.285 real sec...
[M::process] read 673096 sequences (100000227 bp) in 1.100 CPU sec, 1.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284680, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (271.17, 143.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673248 reads in 178.038 CPU sec, 5.712 real sec
[M::mem_process_seqs] Processed 673154 reads in 103.687 CPU sec, 3.347 real sec
sorting block 66, #reads = 1078582 mem = 536876891 peak = 994734080
[M::process] read 672978 sequences (100000251 bp) in 1.960 CPU sec, 2.188 real sec...
[M::process] read 672832 sequences (100000009 bp) in 1.146 CPU sec, 1.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284724, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (276.44, 147.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 198, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1461)
[M::mem_pestat] mean and std.dev: (260.38, 265.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1947)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672978 reads in 98.074 CPU sec, 3.150 real sec
sorting block 67, #reads = 1079531 mem = 536877993 peak = 994734080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284601, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.41, 144.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673474 sequences (100000230 bp) in 1.150 CPU sec, 1.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284933, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.70, 146.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673186 reads in 278.778 CPU sec, 8.948 real sec
[M::mem_process_seqs] Processed 673096 reads in 198.257 CPU sec, 6.392 real sec
sorting block 68, #reads = 1079796 mem = 536877891 peak = 994734080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284639, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (281.71, 153.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673410 sequences (100000042 bp) in 2.241 CPU sec, 2.500 real sec...
[M::process] read 673208 sequences (100000218 bp) in 1.080 CPU sec, 1.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285337, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (269.71, 143.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 23, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538)
[M::mem_pestat] mean and std.dev: (80.60, 81.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672832 reads in 175.948 CPU sec, 5.638 real sec
[M::mem_process_seqs] Processed 673474 reads in 100.276 CPU sec, 3.236 real sec
sorting block 69, #reads = 1079336 mem = 536878521 peak = 994734080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285300, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (273.78, 145.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673282 sequences (100000026 bp) in 2.153 CPU sec, 2.372 real sec...
[M::process] read 673094 sequences (100000194 bp) in 1.090 CPU sec, 1.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284872, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.36, 145.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 79, 883)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2617)
[M::mem_pestat] mean and std.dev: (239.94, 345.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3484)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673410 reads in 181.195 CPU sec, 5.807 real sec
sorting block 70, #reads = 1079272 mem = 536878086 peak = 994734080
[M::mem_process_seqs] Processed 673208 reads in 104.663 CPU sec, 3.384 real sec
sorting block 71, #reads = 1079724 mem = 536877614 peak = 1014603776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284903, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 673086 sequences (100000259 bp) in 1.949 CPU sec, 2.172 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.92, 147.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673284 sequences (100000052 bp) in 1.100 CPU sec, 1.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284511, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (275.97, 145.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673282 reads in 179.791 CPU sec, 5.769 real sec
[M::mem_process_seqs] Processed 673094 reads in 100.989 CPU sec, 3.259 real sec
sorting block 72, #reads = 1079213 mem = 536878468 peak = 1014603776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284394, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (275.66, 145.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673318 sequences (100000205 bp) in 2.139 CPU sec, 2.365 real sec...
[M::process] read 673328 sequences (100000175 bp) in 1.090 CPU sec, 1.094 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285327, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.77, 133.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673086 reads in 174.629 CPU sec, 5.591 real sec
[M::mem_process_seqs] Processed 673284 reads in 98.390 CPU sec, 3.175 real sec
sorting block 73, #reads = 1078420 mem = 536877223 peak = 1014603776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285158, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.96, 138.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 15, 1109)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3301)
[M::mem_pestat] mean and std.dev: (282.27, 413.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4397)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673184 sequences (100000230 bp) in 2.105 CPU sec, 2.371 real sec...
[M::process] read 673156 sequences (100000186 bp) in 1.090 CPU sec, 1.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284360, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.06, 140.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673318 reads in 182.572 CPU sec, 5.854 real sec
[M::mem_process_seqs] Processed 673328 reads in 103.211 CPU sec, 3.323 real sec
sorting block 74, #reads = 1079776 mem = 536878195 peak = 1014603776
sorting block 75, #reads = 1079925 mem = 536880029 peak = 1014603776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284225, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.25, 141.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673142 sequences (100000040 bp) in 2.246 CPU sec, 2.513 real sec...
[M::process] read 672984 sequences (100000294 bp) in 1.250 CPU sec, 1.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284701, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.04, 142.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673184 reads in 185.284 CPU sec, 5.930 real sec
[M::mem_process_seqs] Processed 673156 reads in 104.401 CPU sec, 3.357 real sec
sorting block 76, #reads = 1079769 mem = 536878773 peak = 1014603776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283851, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.19, 144.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673012 sequences (100000254 bp) in 2.153 CPU sec, 2.406 real sec...
[M::process] read 672880 sequences (100000045 bp) in 1.250 CPU sec, 1.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284230, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (277.04, 147.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 374, 2609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7747)
[M::mem_pestat] mean and std.dev: (1084.60, 1387.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10316)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673142 reads in 193.240 CPU sec, 6.201 real sec
[M::mem_process_seqs] Processed 672984 reads in 114.094 CPU sec, 3.696 real sec
sorting block 77, #reads = 1079288 mem = 536878133 peak = 1014603776
[M::process] read 555548 sequences (82567522 bp) in 2.144 CPU sec, 2.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284820, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (277.41, 147.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284161, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (278.74, 148.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673012 reads in 195.846 CPU sec, 6.333 real sec
[M::mem_process_seqs] Processed 672880 reads in 116.401 CPU sec, 3.813 real sec
sorting block 78, #reads = 1079741 mem = 536879728 peak = 1014603776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 234389, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (284.12, 154.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (881, 1155, 4885)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12893)
[M::mem_pestat] mean and std.dev: (1906.33, 1810.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16897)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 555548 reads in 86.955 CPU sec, 2.899 real sec
sorting block 79, #reads = 1079806 mem = 536879187 peak = 1014603776
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 86047296
overall: 69676077056 mem 14406.964 user 1015.693 sys 936.034 real
sorting block 80, #reads = 324395 mem = 161446478 peak = 1014603776
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L001\tPU:L001\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.T18-D0003255_R1.fastq.gz 0004.T18-D0003255_R2.fastq.gz
[main] Real time: 936.113 sec; CPU: 15422.704 sec
uniq header count 1
merging 81 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 3324206712
output reads: 86682269
bam_mem_sort: 81 calls 39.848 user 0.574 sys 40.531 real
bam_write: 81 calls 30.872 user 1.826 sys 33.006 real
execute: 1 calls 19.042 user 1.333 sys 957.850 real
merge_files: 1 calls 19.035 user 1.295 sys 21.427 real
parse_chunk: 1285 calls 48.465 user 13.524 sys 62.921 real
read_chunk: 3459 calls 36.817 user 30.757 sys 438.985 real
sort_block: 1 calls 28.798 user 30.114 sys 426.909 real
write_chunk: 2172 calls 18.683 user 1.050 sys 20.366 real
overall: 2401644544 mem 775.292 user 60.124 sys 957.870 real
ls: cannot access '*.cram': No such file or directory