This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1832.056 CPU sec, 539.470 real sec
[M::process] read 673960 sequences (100000157 bp) in 1.301 CPU sec, 1.304 real sec...
[M::process] read 673854 sequences (100000112 bp) in 1.379 CPU sec, 1.383 real sec...
[M::process] read 673748 sequences (100000190 bp) in 1.500 CPU sec, 1.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286764, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (249.94, 121.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673692 sequences (100000203 bp) in 1.420 CPU sec, 1.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286408, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (253.40, 124.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287062, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (255.31, 126.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673960 reads in 316.547 CPU sec, 10.100 real sec
[M::mem_process_seqs] Processed 673854 reads in 218.797 CPU sec, 7.060 real sec
[M::mem_process_seqs] Processed 673748 reads in 228.183 CPU sec, 7.383 real sec
sorting block 0, #reads = 1080724 mem = 536878367 peak = 606375936
[M::process] read 673592 sequences (100000134 bp) in 2.246 CPU sec, 2.345 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285806, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (256.86, 127.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 91, 1366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4034)
[M::mem_pestat] mean and std.dev: (447.82, 558.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5368)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673486 sequences (100000098 bp) in 1.908 CPU sec, 1.906 real sec...
[M::process] read 673360 sequences (100000079 bp) in 1.910 CPU sec, 1.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286188, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.96, 129.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 675, 1566)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4454)
[M::mem_pestat] mean and std.dev: (617.88, 731.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5898)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673692 reads in 230.843 CPU sec, 7.323 real sec
sorting block 1, #reads = 1080085 mem = 536880678 peak = 916979712
[M::mem_process_seqs] Processed 673592 reads in 128.849 CPU sec, 4.071 real sec
sorting block 2, #reads = 1080394 mem = 536877917 peak = 957308928
[M::process] read 673350 sequences (100000033 bp) in 2.746 CPU sec, 2.820 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285838, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (260.21, 131.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 597, 1269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3741)
[M::mem_pestat] mean and std.dev: (530.50, 492.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4977)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673272 sequences (100000160 bp) in 1.880 CPU sec, 1.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285291, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (262.04, 131.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285068, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.34, 135.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 242, 1122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3290)
[M::mem_pestat] mean and std.dev: (481.27, 494.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4374)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673486 reads in 335.714 CPU sec, 10.718 real sec
[M::mem_process_seqs] Processed 673360 reads in 235.299 CPU sec, 7.538 real sec
[M::mem_process_seqs] Processed 673350 reads in 245.295 CPU sec, 7.876 real sec
sorting block 3, #reads = 1080179 mem = 536879187 peak = 957308928
[M::process] read 673280 sequences (100000029 bp) in 2.948 CPU sec, 3.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284842, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.60, 135.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (359, 792, 999)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2279)
[M::mem_pestat] mean and std.dev: (720.27, 502.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2919)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 4, #reads = 1080365 mem = 536880645 peak = 957308928
[M::process] read 673078 sequences (100000151 bp) in 2.103 CPU sec, 2.171 real sec...
[M::process] read 672934 sequences (100000117 bp) in 1.910 CPU sec, 1.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284976, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (267.32, 135.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673272 reads in 237.181 CPU sec, 7.514 real sec
[M::mem_process_seqs] Processed 673280 reads in 132.441 CPU sec, 4.160 real sec
sorting block 5, #reads = 1079534 mem = 536879458 peak = 957308928
[M::process] read 673046 sequences (100000216 bp) in 2.836 CPU sec, 2.906 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284349, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.87, 138.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672954 sequences (100000160 bp) in 1.940 CPU sec, 1.937 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284499, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.73, 139.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 65, 120)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324)
[M::mem_pestat] mean and std.dev: (42.62, 38.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 426)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284918, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.77, 141.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 168, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (177.50, 140.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673078 reads in 347.835 CPU sec, 11.106 real sec
[M::mem_process_seqs] Processed 672934 reads in 240.943 CPU sec, 7.720 real sec
[M::mem_process_seqs] Processed 673046 reads in 250.423 CPU sec, 8.040 real sec
sorting block 6, #reads = 1079506 mem = 536880872 peak = 957308928
[M::process] read 672684 sequences (100000091 bp) in 2.301 CPU sec, 2.425 real sec...
sorting block 7, #reads = 1079671 mem = 536879287 peak = 1012215808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284968, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.12, 142.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672782 sequences (100000299 bp) in 1.848 CPU sec, 1.899 real sec...
[M::process] read 673642 sequences (100000164 bp) in 1.420 CPU sec, 1.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284689, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.27, 147.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 65, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (68.89, 70.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 660)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672954 reads in 234.301 CPU sec, 7.458 real sec
[M::mem_process_seqs] Processed 672684 reads in 129.954 CPU sec, 4.126 real sec
sorting block 8, #reads = 1079340 mem = 536877257 peak = 1012215808
[M::process] read 673816 sequences (100000013 bp) in 3.092 CPU sec, 3.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285097, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.88, 148.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673724 sequences (100000028 bp) in 2.050 CPU sec, 2.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286135, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (254.09, 125.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285752, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.52, 130.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672782 reads in 324.543 CPU sec, 10.342 real sec
[M::mem_process_seqs] Processed 673816 reads in 220.075 CPU sec, 7.050 real sec
[M::mem_process_seqs] Processed 673642 reads in 229.323 CPU sec, 7.368 real sec
sorting block 9, #reads = 1079224 mem = 536881765 peak = 1012215808
[M::process] read 673668 sequences (100000092 bp) in 2.502 CPU sec, 2.671 real sec...
sorting block 10, #reads = 1079786 mem = 536878814 peak = 1012215808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285780, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.76, 127.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 148, 768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2258)
[M::mem_pestat] mean and std.dev: (302.08, 425.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3003)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673598 sequences (100000283 bp) in 1.660 CPU sec, 1.678 real sec...
[M::process] read 673594 sequences (100000263 bp) in 1.400 CPU sec, 1.400 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286400, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.14, 127.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 148, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (122.69, 116.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673724 reads in 198.788 CPU sec, 6.344 real sec
[M::mem_process_seqs] Processed 673668 reads in 111.921 CPU sec, 3.546 real sec
sorting block 11, #reads = 1080331 mem = 536881001 peak = 1012215808
[M::process] read 673334 sequences (100000239 bp) in 2.132 CPU sec, 2.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285933, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.52, 130.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673270 sequences (100000153 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285616, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.89, 133.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285532, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.91, 130.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673598 reads in 318.531 CPU sec, 10.197 real sec
[M::mem_process_seqs] Processed 673334 reads in 223.378 CPU sec, 7.190 real sec
sorting block 12, #reads = 1080553 mem = 536879724 peak = 1012215808
[M::mem_process_seqs] Processed 673594 reads in 233.183 CPU sec, 7.522 real sec
sorting block 13, #reads = 1080180 mem = 536877404 peak = 1012215808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285471, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.44, 137.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 47, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280)
[M::mem_pestat] mean and std.dev: (40.29, 31.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 369)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673294 sequences (100000294 bp) in 3.513 CPU sec, 3.781 real sec...
[M::mem_process_seqs] Processed 673270 reads in 122.450 CPU sec, 4.027 real sec
sorting block 14, #reads = 1079930 mem = 536880952 peak = 1028116480
[M::process] read 673098 sequences (100000220 bp) in 2.571 CPU sec, 2.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285407, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.04, 137.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673128 sequences (100000023 bp) in 2.000 CPU sec, 2.000 real sec...
[M::process] read 672996 sequences (100000127 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285353, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.88, 137.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673294 reads in 227.859 CPU sec, 7.192 real sec
[M::process] read 672976 sequences (100000173 bp) in 1.738 CPU sec, 1.740 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285252, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.17, 138.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 91, 561)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1647)
[M::mem_pestat] mean and std.dev: (285.94, 269.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2190)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673128 reads in 121.346 CPU sec, 4.014 real sec
sorting block 15, #reads = 1079694 mem = 536879922 peak = 1028116480
[M::mem_process_seqs] Processed 673098 reads in 267.077 CPU sec, 8.640 real sec
[M::process] read 672966 sequences (100000279 bp) in 2.527 CPU sec, 2.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285233, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (271.16, 138.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672862 sequences (100000173 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284666, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.05, 140.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285386, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.12, 143.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672996 reads in 347.031 CPU sec, 11.100 real sec
sorting block 16, #reads = 1080029 mem = 536882021 peak = 1033732096
[M::mem_process_seqs] Processed 672976 reads in 233.605 CPU sec, 7.505 real sec
[M::mem_process_seqs] Processed 672966 reads in 243.155 CPU sec, 7.829 real sec
sorting block 17, #reads = 1079486 mem = 536880661 peak = 1091612672
[M::process] read 672842 sequences (100000134 bp) in 2.223 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285389, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.49, 147.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 365, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (203.11, 201.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1385)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673342 sequences (100000182 bp) in 2.007 CPU sec, 2.068 real sec...
[M::process] read 673958 sequences (100000248 bp) in 1.400 CPU sec, 1.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284804, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.39, 148.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672862 reads in 208.141 CPU sec, 6.622 real sec
[M::mem_process_seqs] Processed 672842 reads in 113.944 CPU sec, 3.616 real sec
sorting block 18, #reads = 1079557 mem = 536879827 peak = 1091612672
sorting block 19, #reads = 1079769 mem = 536879050 peak = 1109557248
[M::process] read 673842 sequences (100000093 bp) in 1.918 CPU sec, 1.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285879, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.17, 138.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 92, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358)
[M::mem_pestat] mean and std.dev: (53.27, 45.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673704 sequences (100000120 bp) in 1.460 CPU sec, 1.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286558, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (255.37, 127.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286843, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (254.87, 126.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 65, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1343)
[M::mem_pestat] mean and std.dev: (190.27, 249.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1784)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673342 reads in 277.354 CPU sec, 8.880 real sec
[M::mem_process_seqs] Processed 673842 reads in 192.641 CPU sec, 6.195 real sec
[M::mem_process_seqs] Processed 673958 reads in 201.612 CPU sec, 6.504 real sec
sorting block 20, #reads = 1079839 mem = 536877931 peak = 1109557248
[M::process] read 673674 sequences (100000187 bp) in 2.122 CPU sec, 2.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286159, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.72, 130.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673526 sequences (100000299 bp) in 1.635 CPU sec, 1.649 real sec...
[M::process] read 673458 sequences (100000138 bp) in 1.410 CPU sec, 1.410 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285956, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.63, 129.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 25, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242)
[M::mem_pestat] mean and std.dev: (44.33, 54.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 318)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673704 reads in 194.567 CPU sec, 6.194 real sec
sorting block 21, #reads = 1080366 mem = 536878679 peak = 1109557248
[M::mem_process_seqs] Processed 673674 reads in 109.864 CPU sec, 3.487 real sec
sorting block 22, #reads = 1079951 mem = 536877752 peak = 1110171648
[M::process] read 673400 sequences (100000265 bp) in 1.898 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285844, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.42, 132.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673332 sequences (100000180 bp) in 1.420 CPU sec, 1.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285269, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.69, 132.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 148, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (141.30, 99.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285359, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.14, 136.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 5, 741)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2217)
[M::mem_pestat] mean and std.dev: (76.82, 210.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2955)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673526 reads in 293.392 CPU sec, 9.533 real sec
[M::mem_process_seqs] Processed 673458 reads in 202.877 CPU sec, 6.539 real sec
[M::mem_process_seqs] Processed 673400 reads in 211.963 CPU sec, 6.853 real sec
sorting block 23, #reads = 1079934 mem = 536877396 peak = 1110171648
[M::process] read 673292 sequences (100000013 bp) in 1.996 CPU sec, 2.077 real sec...
sorting block 24, #reads = 1079588 mem = 536878394 peak = 1131778048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285152, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.48, 138.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673200 sequences (100000048 bp) in 1.679 CPU sec, 1.692 real sec...
[M::process] read 673076 sequences (100000241 bp) in 1.440 CPU sec, 1.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284926, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.28, 139.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 34, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1276)
[M::mem_pestat] mean and std.dev: (177.15, 261.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1694)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673332 reads in 200.979 CPU sec, 6.387 real sec
[M::mem_process_seqs] Processed 673292 reads in 112.222 CPU sec, 3.577 real sec
sorting block 25, #reads = 1079678 mem = 536877867 peak = 1131778048
[M::process] read 673034 sequences (100000262 bp) in 2.338 CPU sec, 2.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285143, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.26, 141.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672918 sequences (100000193 bp) in 1.430 CPU sec, 1.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284927, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (273.14, 142.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284723, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (274.70, 142.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673200 reads in 292.260 CPU sec, 9.358 real sec
[M::mem_process_seqs] Processed 673076 reads in 204.910 CPU sec, 6.578 real sec
[M::mem_process_seqs] Processed 673034 reads in 213.948 CPU sec, 6.894 real sec
sorting block 26, #reads = 1079752 mem = 536879925 peak = 1131778048
sorting block 27, #reads = 1079957 mem = 536878412 peak = 1131778048
[M::process] read 672816 sequences (100000263 bp) in 2.190 CPU sec, 2.266 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285095, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.53, 144.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672746 sequences (100000026 bp) in 1.652 CPU sec, 1.666 real sec...
[M::process] read 672988 sequences (100000138 bp) in 1.470 CPU sec, 1.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285230, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.75, 144.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 539, 1131)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3303)
[M::mem_pestat] mean and std.dev: (451.42, 451.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4389)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672918 reads in 201.676 CPU sec, 6.417 real sec
[M::mem_process_seqs] Processed 672816 reads in 112.612 CPU sec, 3.580 real sec
sorting block 28, #reads = 1079560 mem = 536879359 peak = 1131778048
[M::process] read 673818 sequences (100000242 bp) in 1.834 CPU sec, 1.899 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284995, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.84, 148.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673762 sequences (100000296 bp) in 1.430 CPU sec, 1.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285510, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (277.76, 145.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (65, 191, 651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1823)
[M::mem_pestat] mean and std.dev: (385.47, 496.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2409)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286289, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (252.74, 123.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 63, 98)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288)
[M::mem_pestat] mean and std.dev: (40.12, 36.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 383)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672746 reads in 284.733 CPU sec, 9.214 real sec
[M::mem_process_seqs] Processed 672988 reads in 195.225 CPU sec, 6.285 real sec
[M::mem_process_seqs] Processed 673818 reads in 204.184 CPU sec, 6.596 real sec
sorting block 29, #reads = 1079347 mem = 536880507 peak = 1131778048
sorting block 30, #reads = 1079971 mem = 536879158 peak = 1131778048
[M::process] read 673572 sequences (100000270 bp) in 2.273 CPU sec, 2.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286411, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (253.34, 124.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 432, 1392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3916)
[M::mem_pestat] mean and std.dev: (596.67, 588.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5178)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673702 sequences (100000127 bp) in 1.749 CPU sec, 1.771 real sec...
[M::process] read 673626 sequences (100000256 bp) in 1.461 CPU sec, 1.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285782, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.61, 128.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673762 reads in 198.710 CPU sec, 6.325 real sec
[M::mem_process_seqs] Processed 673572 reads in 111.070 CPU sec, 3.524 real sec
sorting block 31, #reads = 1080233 mem = 536879797 peak = 1131778048
[M::process] read 673506 sequences (100000217 bp) in 2.124 CPU sec, 2.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285741, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (256.84, 127.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673348 sequences (100000293 bp) in 1.410 CPU sec, 1.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285678, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.82, 129.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285152, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (261.15, 130.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673702 reads in 287.675 CPU sec, 9.206 real sec
[M::mem_process_seqs] Processed 673626 reads in 201.795 CPU sec, 6.488 real sec
sorting block 32, #reads = 1080454 mem = 536880221 peak = 1131778048
[M::mem_process_seqs] Processed 673506 reads in 210.827 CPU sec, 6.800 real sec
sorting block 33, #reads = 1080184 mem = 536877368 peak = 1131778048
[M::process] read 673278 sequences (100000009 bp) in 2.488 CPU sec, 2.638 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285147, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.49, 132.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673142 sequences (100000030 bp) in 1.787 CPU sec, 1.822 real sec...
[M::process] read 673190 sequences (100000002 bp) in 1.470 CPU sec, 1.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285661, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.87, 135.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 374, 733)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2173)
[M::mem_pestat] mean and std.dev: (386.12, 397.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2893)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673348 reads in 201.291 CPU sec, 6.408 real sec
[M::mem_process_seqs] Processed 673278 reads in 112.795 CPU sec, 3.574 real sec
sorting block 34, #reads = 1079984 mem = 536879547 peak = 1131778048
[M::process] read 672984 sequences (100000229 bp) in 1.944 CPU sec, 2.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285369, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.58, 137.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672900 sequences (100000228 bp) in 1.430 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285030, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.15, 139.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285332, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.25, 140.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673142 reads in 292.219 CPU sec, 9.368 real sec
[M::mem_process_seqs] Processed 673190 reads in 203.546 CPU sec, 6.562 real sec
sorting block 35, #reads = 1080008 mem = 536880667 peak = 1131778048
[M::mem_process_seqs] Processed 672984 reads in 212.626 CPU sec, 6.877 real sec
sorting block 36, #reads = 1079809 mem = 536879511 peak = 1131778048
[M::process] read 672812 sequences (100000099 bp) in 2.586 CPU sec, 2.768 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284788, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.92, 142.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 157)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275)
[M::mem_pestat] mean and std.dev: (103.40, 50.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 334)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672832 sequences (100000099 bp) in 1.970 CPU sec, 2.069 real sec...
[M::process] read 672772 sequences (100000145 bp) in 1.460 CPU sec, 1.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284564, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.69, 144.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 226, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (147.67, 185.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672900 reads in 211.064 CPU sec, 6.720 real sec
[M::mem_process_seqs] Processed 672812 reads in 117.130 CPU sec, 3.713 real sec
sorting block 37, #reads = 1080091 mem = 536880351 peak = 1131778048
[M::process] read 672624 sequences (100000000 bp) in 2.049 CPU sec, 2.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285235, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.93, 145.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 754, 1717)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4671)
[M::mem_pestat] mean and std.dev: (800.00, 694.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6148)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673660 sequences (100000161 bp) in 1.460 CPU sec, 1.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284614, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.14, 145.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 18, 75)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 195)
[M::mem_pestat] mean and std.dev: (35.42, 33.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 255)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285134, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (283.17, 148.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672832 reads in 292.918 CPU sec, 9.377 real sec
sorting block 38, #reads = 1078997 mem = 536878442 peak = 1154326528
[M::mem_process_seqs] Processed 672772 reads in 202.924 CPU sec, 6.538 real sec
[M::mem_process_seqs] Processed 672624 reads in 212.124 CPU sec, 6.857 real sec
sorting block 39, #reads = 1079693 mem = 536877476 peak = 1179807744
[M::process] read 674034 sequences (100000016 bp) in 2.602 CPU sec, 2.758 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285518, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.60, 131.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 20, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (99.79, 103.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673942 sequences (100000211 bp) in 2.043 CPU sec, 2.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285657, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.89, 123.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673786 sequences (100000198 bp) in 1.460 CPU sec, 1.462 real sec...
[M::mem_process_seqs] Processed 673660 reads in 199.721 CPU sec, 6.355 real sec
[M::mem_process_seqs] Processed 674034 reads in 113.952 CPU sec, 3.602 real sec
sorting block 40, #reads = 1079195 mem = 536879733 peak = 1179807744
sorting block 41, #reads = 1080102 mem = 536877238 peak = 1186942976
[M::process] read 673888 sequences (100000109 bp) in 2.330 CPU sec, 2.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285551, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.43, 125.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 2, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (82.31, 122.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673606 sequences (100000135 bp) in 1.440 CPU sec, 1.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285050, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.96, 130.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284490, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (253.72, 127.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673942 reads in 298.689 CPU sec, 9.559 real sec
[M::mem_process_seqs] Processed 673888 reads in 209.163 CPU sec, 6.725 real sec
[M::mem_process_seqs] Processed 673786 reads in 218.111 CPU sec, 7.035 real sec
sorting block 42, #reads = 1080055 mem = 536877875 peak = 1186942976
[M::process] read 673594 sequences (100000271 bp) in 2.105 CPU sec, 2.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285371, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.48, 129.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673542 sequences (100000214 bp) in 1.613 CPU sec, 1.636 real sec...
[M::process] read 673454 sequences (100000025 bp) in 1.460 CPU sec, 1.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284797, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.23, 133.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673606 reads in 208.778 CPU sec, 6.651 real sec
sorting block 43, #reads = 1079663 mem = 536878711 peak = 1186942976
[M::mem_process_seqs] Processed 673594 reads in 116.956 CPU sec, 3.719 real sec
sorting block 44, #reads = 1080357 mem = 536879499 peak = 1215299584
[M::process] read 673262 sequences (100000176 bp) in 1.922 CPU sec, 1.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284931, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.42, 136.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673242 sequences (100000052 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284521, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.70, 137.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284414, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.96, 138.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 24, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 228)
[M::mem_pestat] mean and std.dev: (31.80, 29.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673542 reads in 310.621 CPU sec, 9.952 real sec
[M::mem_process_seqs] Processed 673454 reads in 216.315 CPU sec, 6.976 real sec
[M::mem_process_seqs] Processed 673262 reads in 225.516 CPU sec, 7.293 real sec
sorting block 45, #reads = 1080231 mem = 536881473 peak = 1215299584
[M::process] read 673216 sequences (100000000 bp) in 2.406 CPU sec, 2.517 real sec...
sorting block 46, #reads = 1079959 mem = 536879653 peak = 1215299584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284138, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.15, 141.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673084 sequences (100000128 bp) in 1.960 CPU sec, 2.030 real sec...
[M::process] read 673106 sequences (100000079 bp) in 1.480 CPU sec, 1.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283779, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.21, 141.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 46, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222)
[M::mem_pestat] mean and std.dev: (32.45, 28.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 290)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673242 reads in 217.875 CPU sec, 6.928 real sec
[M::mem_process_seqs] Processed 673216 reads in 120.909 CPU sec, 3.838 real sec
sorting block 47, #reads = 1079699 mem = 536878766 peak = 1215299584
[M::process] read 673054 sequences (100000175 bp) in 2.387 CPU sec, 2.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284145, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.93, 142.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673248 sequences (100000058 bp) in 1.450 CPU sec, 1.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284476, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.44, 143.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 46, 186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538)
[M::mem_pestat] mean and std.dev: (74.92, 74.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284463, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (277.94, 148.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 303, 551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1427)
[M::mem_pestat] mean and std.dev: (322.58, 241.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1865)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673084 reads in 311.173 CPU sec, 9.964 real sec
[M::mem_process_seqs] Processed 673106 reads in 216.300 CPU sec, 6.957 real sec
[M::mem_process_seqs] Processed 673054 reads in 225.514 CPU sec, 7.274 real sec
sorting block 48, #reads = 1079516 mem = 536878219 peak = 1215299584
[M::process] read 674128 sequences (100000159 bp) in 2.504 CPU sec, 2.660 real sec...
sorting block 49, #reads = 1079737 mem = 536879129 peak = 1215299584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284325, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.46, 147.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 161, 1087)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3191)
[M::mem_pestat] mean and std.dev: (545.47, 627.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4243)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674038 sequences (100000214 bp) in 1.965 CPU sec, 2.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286131, 18, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (247.98, 123.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 477, 1132)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3240)
[M::mem_pestat] mean and std.dev: (399.57, 389.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4294)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673928 sequences (100000065 bp) in 1.460 CPU sec, 1.468 real sec...
[M::mem_process_seqs] Processed 673248 reads in 204.828 CPU sec, 6.518 real sec
[M::mem_process_seqs] Processed 674128 reads in 111.530 CPU sec, 3.531 real sec
sorting block 50, #reads = 1079543 mem = 536879872 peak = 1215299584
[M::process] read 673770 sequences (100000272 bp) in 2.002 CPU sec, 2.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285909, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.48, 125.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (103, 103, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1966)
[M::mem_pestat] mean and std.dev: (291.64, 333.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2587)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673722 sequences (100000157 bp) in 1.430 CPU sec, 1.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285458, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (253.47, 127.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285901, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (255.74, 129.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 11, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (104.23, 187.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674038 reads in 285.792 CPU sec, 9.149 real sec
[M::mem_process_seqs] Processed 673928 reads in 200.622 CPU sec, 6.459 real sec
[M::mem_process_seqs] Processed 673770 reads in 209.780 CPU sec, 6.776 real sec
sorting block 51, #reads = 1080578 mem = 536879842 peak = 1215299584
sorting block 52, #reads = 1080161 mem = 536877565 peak = 1215299584
[M::process] read 673660 sequences (100000052 bp) in 2.278 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285671, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.59, 130.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 86, 703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2083)
[M::mem_pestat] mean and std.dev: (233.40, 472.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2773)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673556 sequences (100000110 bp) in 1.656 CPU sec, 1.676 real sec...
[M::process] read 673460 sequences (100000016 bp) in 1.470 CPU sec, 1.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285327, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.39, 130.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673722 reads in 209.845 CPU sec, 6.684 real sec
[M::mem_process_seqs] Processed 673660 reads in 115.894 CPU sec, 3.682 real sec
sorting block 53, #reads = 1079542 mem = 536878762 peak = 1215299584
[M::process] read 673468 sequences (100000054 bp) in 2.439 CPU sec, 2.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284755, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.14, 136.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673330 sequences (100000245 bp) in 1.450 CPU sec, 1.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284324, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.69, 137.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284208, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.38, 139.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673556 reads in 308.496 CPU sec, 9.878 real sec
[M::mem_process_seqs] Processed 673460 reads in 215.825 CPU sec, 6.933 real sec
sorting block 54, #reads = 1079313 mem = 536878011 peak = 1215299584
[M::mem_process_seqs] Processed 673468 reads in 225.101 CPU sec, 7.257 real sec
sorting block 55, #reads = 1079888 mem = 536880115 peak = 1215299584
[M::process] read 673230 sequences (100000260 bp) in 2.303 CPU sec, 2.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284619, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (269.93, 142.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673228 sequences (100000127 bp) in 1.737 CPU sec, 1.782 real sec...
[M::process] read 673082 sequences (100000009 bp) in 1.480 CPU sec, 1.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284628, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.26, 142.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 91, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1445)
[M::mem_pestat] mean and std.dev: (134.33, 148.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1911)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673330 reads in 213.402 CPU sec, 6.797 real sec
[M::mem_process_seqs] Processed 673230 reads in 119.636 CPU sec, 3.801 real sec
sorting block 56, #reads = 1079756 mem = 536880567 peak = 1215299584
[M::process] read 673046 sequences (100000013 bp) in 2.026 CPU sec, 2.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284167, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.74, 145.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673074 sequences (100000103 bp) in 1.420 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284362, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.00, 147.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 49, 79)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231)
[M::mem_pestat] mean and std.dev: (30.22, 28.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 307)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284903, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.52, 145.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 67, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (126.80, 201.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1403)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673228 reads in 306.526 CPU sec, 9.817 real sec
[M::mem_process_seqs] Processed 673046 reads in 213.012 CPU sec, 6.871 real sec
sorting block 57, #reads = 1079787 mem = 536881394 peak = 1215299584
[M::mem_process_seqs] Processed 673082 reads in 222.059 CPU sec, 7.183 real sec
sorting block 58, #reads = 1079291 mem = 536877538 peak = 1215299584
[M::process] read 673836 sequences (100000082 bp) in 2.724 CPU sec, 2.912 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284925, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (277.49, 151.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 205, 733)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2109)
[M::mem_pestat] mean and std.dev: (396.38, 472.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2797)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674234 sequences (100000202 bp) in 1.648 CPU sec, 1.817 real sec...
[M::process] read 673914 sequences (100000086 bp) in 1.510 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285671, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (258.33, 133.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 98, 1004)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2998)
[M::mem_pestat] mean and std.dev: (439.08, 711.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3995)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673074 reads in 205.763 CPU sec, 6.567 real sec
[M::mem_process_seqs] Processed 673836 reads in 112.528 CPU sec, 3.557 real sec
sorting block 59, #reads = 1079940 mem = 536879244 peak = 1215299584
[M::process] read 673926 sequences (100000129 bp) in 1.917 CPU sec, 1.977 real sec...
[M::process] read 673794 sequences (100000028 bp) in 1.410 CPU sec, 1.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285830, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (253.70, 127.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673914 reads in 101.393 CPU sec, 3.251 real sec
sorting block 60, #reads = 1079673 mem = 536877964 peak = 1215299584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286140, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.47, 126.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673618 sequences (100000011 bp) in 1.501 CPU sec, 1.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285837, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (254.74, 128.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 41, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (64.89, 70.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674234 reads in 296.735 CPU sec, 9.466 real sec
[M::mem_process_seqs] Processed 673926 reads in 210.791 CPU sec, 6.752 real sec
sorting block 61, #reads = 1080218 mem = 536879319 peak = 1215299584
[M::process] read 673626 sequences (100000213 bp) in 2.171 CPU sec, 2.262 real sec...
[M::process] read 673446 sequences (100000276 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285669, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (257.90, 131.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 92, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (158.20, 182.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673618 reads in 103.163 CPU sec, 3.302 real sec
sorting block 62, #reads = 1080429 mem = 536877686 peak = 1215299584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286343, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (256.99, 130.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673430 sequences (100000002 bp) in 1.434 CPU sec, 1.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285595, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.36, 136.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (37, 91, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078)
[M::mem_pestat] mean and std.dev: (250.30, 314.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1508)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673794 reads in 300.849 CPU sec, 9.598 real sec
[M::mem_process_seqs] Processed 673626 reads in 213.102 CPU sec, 6.821 real sec
sorting block 63, #reads = 1080252 mem = 536877855 peak = 1215299584
[M::process] read 673286 sequences (100000298 bp) in 1.958 CPU sec, 2.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285133, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (263.87, 136.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673350 sequences (100000220 bp) in 1.400 CPU sec, 1.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284859, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (265.98, 138.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284942, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.05, 140.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673446 reads in 299.189 CPU sec, 9.583 real sec
[M::mem_process_seqs] Processed 673430 reads in 208.887 CPU sec, 6.734 real sec
sorting block 64, #reads = 1080421 mem = 536881828 peak = 1215299584
[M::mem_process_seqs] Processed 673286 reads in 218.105 CPU sec, 7.057 real sec
sorting block 65, #reads = 1079941 mem = 536880232 peak = 1215299584
[M::process] read 673242 sequences (100000261 bp) in 2.749 CPU sec, 2.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284953, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.21, 142.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673184 sequences (100000050 bp) in 1.828 CPU sec, 1.868 real sec...
[M::process] read 673088 sequences (100000247 bp) in 1.480 CPU sec, 1.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284925, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.70, 144.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673350 reads in 208.781 CPU sec, 6.654 real sec
[M::mem_process_seqs] Processed 673242 reads in 116.649 CPU sec, 3.694 real sec
sorting block 66, #reads = 1079904 mem = 536878675 peak = 1215299584
[M::process] read 673024 sequences (100000295 bp) in 2.215 CPU sec, 2.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284867, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.05, 147.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673084 sequences (100000099 bp) in 1.460 CPU sec, 1.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284911, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.38, 146.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 88, 590)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1706)
[M::mem_pestat] mean and std.dev: (257.30, 324.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2264)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284876, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (278.04, 150.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673184 reads in 297.922 CPU sec, 9.535 real sec
[M::mem_process_seqs] Processed 673088 reads in 207.491 CPU sec, 6.683 real sec
sorting block 67, #reads = 1079907 mem = 536879866 peak = 1215299584
[M::mem_process_seqs] Processed 673024 reads in 216.971 CPU sec, 7.221 real sec
sorting block 68, #reads = 1079804 mem = 536878676 peak = 1215299584
[M::process] read 674242 sequences (100000099 bp) in 2.348 CPU sec, 2.460 real sec...
[M::process] read 674028 sequences (100000010 bp) in 1.944 CPU sec, 2.008 real sec...
[M::process] read 673920 sequences (100000038 bp) in 1.500 CPU sec, 1.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286019, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.06, 124.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674242 reads in 108.768 CPU sec, 3.441 real sec
sorting block 69, #reads = 1079847 mem = 536878667 peak = 1215299584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285159, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (276.91, 149.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 608, 1717)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4835)
[M::mem_pestat] mean and std.dev: (798.33, 797.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6394)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673808 sequences (100000166 bp) in 1.596 CPU sec, 1.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285793, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.44, 125.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673084 reads in 304.666 CPU sec, 9.694 real sec
[M::mem_process_seqs] Processed 674028 reads in 122.222 CPU sec, 3.919 real sec
sorting block 70, #reads = 1079825 mem = 536879190 peak = 1215299584
[M::process] read 673880 sequences (100000281 bp) in 2.088 CPU sec, 2.132 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285476, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (254.63, 128.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673596 sequences (100000261 bp) in 1.430 CPU sec, 1.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284486, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.77, 131.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285359, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.77, 129.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673920 reads in 294.737 CPU sec, 9.434 real sec
[M::mem_process_seqs] Processed 673880 reads in 206.841 CPU sec, 6.660 real sec
sorting block 71, #reads = 1080423 mem = 536877251 peak = 1215299584
[M::mem_process_seqs] Processed 673808 reads in 215.782 CPU sec, 6.968 real sec
sorting block 72, #reads = 1079887 mem = 536877148 peak = 1215299584
[M::process] read 673478 sequences (100000219 bp) in 2.342 CPU sec, 2.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284781, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.90, 134.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673424 sequences (100000072 bp) in 1.703 CPU sec, 1.714 real sec...
[M::process] read 673302 sequences (100000198 bp) in 1.460 CPU sec, 1.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285054, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.73, 137.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673596 reads in 208.628 CPU sec, 6.649 real sec
[M::mem_process_seqs] Processed 673478 reads in 114.940 CPU sec, 3.654 real sec
sorting block 73, #reads = 1080052 mem = 536879169 peak = 1215299584
[M::process] read 673356 sequences (100000213 bp) in 2.728 CPU sec, 2.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284478, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.37, 138.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 25, 653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1933)
[M::mem_pestat] mean and std.dev: (243.10, 353.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2573)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673160 sequences (100000166 bp) in 1.450 CPU sec, 1.446 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284751, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.37, 140.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 191, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2282)
[M::mem_pestat] mean and std.dev: (333.58, 429.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3036)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284435, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.07, 141.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 128, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1506)
[M::mem_pestat] mean and std.dev: (181.08, 215.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2005)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673424 reads in 307.125 CPU sec, 9.828 real sec
[M::mem_process_seqs] Processed 673302 reads in 214.498 CPU sec, 6.894 real sec
sorting block 74, #reads = 1079899 mem = 536876865 peak = 1215299584
[M::mem_process_seqs] Processed 673356 reads in 223.800 CPU sec, 7.214 real sec
sorting block 75, #reads = 1080132 mem = 536879525 peak = 1215299584
[M::process] read 673120 sequences (100000270 bp) in 2.105 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284485, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.86, 143.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673082 sequences (100000171 bp) in 1.695 CPU sec, 1.711 real sec...
[M::process] read 672940 sequences (100000066 bp) in 1.470 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284820, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.06, 144.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673160 reads in 213.428 CPU sec, 6.788 real sec
sorting block 76, #reads = 1079620 mem = 536877726 peak = 1215299584
[M::process] read 672946 sequences (100000158 bp) in 1.427 CPU sec, 1.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283953, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (276.45, 146.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673082 reads in 115.201 CPU sec, 3.813 real sec
[M::mem_process_seqs] Processed 673120 reads in 255.436 CPU sec, 8.293 real sec
sorting block 77, #reads = 1079603 mem = 536877923 peak = 1215299584
[M::process] read 376146 sequences (55895686 bp) in 1.423 CPU sec, 1.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284540, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (279.13, 149.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284289, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (282.27, 153.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 159249, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (279.78, 151.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672940 reads in 267.678 CPU sec, 8.616 real sec
[M::mem_process_seqs] Processed 672946 reads in 166.146 CPU sec, 5.378 real sec
[M::mem_process_seqs] Processed 376146 reads in 171.425 CPU sec, 5.560 real sec
sorting block 78, #reads = 1079939 mem = 536883203 peak = 1215299584
sorting block 79, #reads = 1078852 mem = 536882899 peak = 1276874752
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 85897002
overall: 69935779840 mem 15415.446 user 274.606 sys 1001.917 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L007\tPU:L007\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.T18-D0003255_R1.fastq.gz 0001.T18-D0003255_R2.fastq.gz
[main] Real time: 1001.951 sec; CPU: 15690.056 sec
sorting block 80, #reads = 132044 mem = 65719553 peak = 1276874752
uniq header count 1
merging 81 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31 max 96
algo: util-sort
license: sentieon:util=1
output file size: 3305155690
output reads: 86522718
bam_mem_sort: 81 calls 62.015 user 0.795 sys 62.949 real
bam_write: 81 calls 21.005 user 0.677 sys 21.427 real
execute: 1 calls 23.710 user 0.960 sys 1027.766 real
merge_files: 1 calls 23.684 user 0.928 sys 25.553 real
parse_chunk: 1282 calls 84.263 user 17.301 sys 102.612 real
read_chunk: 3451 calls 33.029 user 24.215 sys 467.201 real
sort_block: 1 calls 21.234 user 23.781 sys 451.260 real
write_chunk: 2167 calls 23.036 user 0.701 sys 24.236 real
overall: 2597167104 mem 1064.493 user 56.887 sys 1027.788 real
ls: cannot access '*.cram': No such file or directory