.command.err - Workdir - DAQ:SENTIEON_BWAMEM (T21-D0003322;L005;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b4/fea81368bb70ca6bf8b878068cdb86/.command.err
Size: 346.3 KB
Task: DAQ:SENTIEON_BWAMEM (T21-D0003322;L005;0001)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003322.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1790.716 CPU sec, 61.378 real sec
[M::process] read 675454 sequences (100000072 bp) in 1.249 CPU sec, 1.250 real sec...
[M::process] read 675134 sequences (100000100 bp) in 1.872 CPU sec, 1.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286416, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.23, 122.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 13, 128)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358)
[M::mem_pestat] mean and std.dev: (51.56, 58.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 473)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675252 sequences (100000007 bp) in 1.903 CPU sec, 1.917 real sec...
[M::process] read 675278 sequences (100000123 bp) in 1.907 CPU sec, 1.916 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285619, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.29, 124.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 158, 652)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1950)
[M::mem_pestat] mean and std.dev: (306.62, 448.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2599)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675454 reads in 238.290 CPU sec, 7.531 real sec
[M::mem_process_seqs] Processed 675134 reads in 135.366 CPU sec, 4.316 real sec
sorting block 0, #reads = 1080516 mem = 536877389 peak = 684138496
[M::process] read 675066 sequences (100000217 bp) in 1.961 CPU sec, 2.381 real sec...
[M::process] read 674964 sequences (100000179 bp) in 1.760 CPU sec, 1.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285696, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.61, 128.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 53, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430)
[M::mem_pestat] mean and std.dev: (241.46, 234.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1898)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675278 reads in 125.556 CPU sec, 4.075 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285727, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.85, 126.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675046 sequences (100000088 bp) in 1.825 CPU sec, 1.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285744, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.60, 127.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675252 reads in 365.902 CPU sec, 11.884 real sec
sorting block 1, #reads = 1079844 mem = 536880201 peak = 815472640
[M::mem_process_seqs] Processed 675066 reads in 261.047 CPU sec, 8.448 real sec
sorting block 2, #reads = 1080132 mem = 536877743 peak = 994725888
[M::process] read 675106 sequences (100000214 bp) in 2.090 CPU sec, 2.753 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285651, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.80, 127.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 614, 619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831)
[M::mem_pestat] mean and std.dev: (384.90, 392.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2437)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674912 sequences (100000205 bp) in 1.735 CPU sec, 1.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285702, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.55, 129.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286354, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (254.28, 126.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 11, 58)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 152)
[M::mem_pestat] mean and std.dev: (20.00, 23.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 199)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674964 reads in 357.395 CPU sec, 11.666 real sec
[M::mem_process_seqs] Processed 675046 reads in 250.503 CPU sec, 8.096 real sec
[M::mem_process_seqs] Processed 675106 reads in 261.422 CPU sec, 8.476 real sec
sorting block 3, #reads = 1080567 mem = 536879671 peak = 994725888
[M::process] read 674888 sequences (100000035 bp) in 2.124 CPU sec, 2.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285633, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.98, 128.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674980 sequences (100000109 bp) in 1.915 CPU sec, 2.288 real sec...
sorting block 4, #reads = 1080602 mem = 536879908 peak = 1020268544
[M::process] read 675384 sequences (100000108 bp) in 1.763 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285827, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.99, 128.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674912 reads in 248.215 CPU sec, 8.126 real sec
[M::mem_process_seqs] Processed 674888 reads in 139.658 CPU sec, 4.536 real sec
sorting block 5, #reads = 1080110 mem = 536879409 peak = 1020268544
[M::process] read 675504 sequences (100000224 bp) in 2.157 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285817, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.85, 128.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 247, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (152.11, 111.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675306 sequences (100000254 bp) in 1.785 CPU sec, 1.829 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286001, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.32, 122.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286085, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.90, 126.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674980 reads in 355.595 CPU sec, 11.573 real sec
[M::mem_process_seqs] Processed 675504 reads in 248.234 CPU sec, 8.027 real sec
[M::mem_process_seqs] Processed 675384 reads in 259.044 CPU sec, 8.396 real sec
sorting block 6, #reads = 1080580 mem = 536879748 peak = 1020268544
[M::process] read 675156 sequences (100000248 bp) in 2.308 CPU sec, 2.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285816, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.84, 124.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 245, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079)
[M::mem_pestat] mean and std.dev: (218.20, 195.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1430)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 7, #reads = 1080558 mem = 536877055 peak = 1043800064
[M::process] read 675244 sequences (100000188 bp) in 2.123 CPU sec, 2.604 real sec...
[M::process] read 675290 sequences (100000262 bp) in 1.812 CPU sec, 1.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286010, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.86, 125.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675306 reads in 252.524 CPU sec, 8.283 real sec
[M::mem_process_seqs] Processed 675156 reads in 142.679 CPU sec, 4.656 real sec
sorting block 8, #reads = 1081120 mem = 536878821 peak = 1043800064
[M::process] read 675216 sequences (100000003 bp) in 2.160 CPU sec, 2.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285578, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.64, 125.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675152 sequences (100000162 bp) in 1.808 CPU sec, 1.829 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285661, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.03, 126.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285990, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.68, 125.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 42, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193)
[M::mem_pestat] mean and std.dev: (32.47, 22.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 253)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675244 reads in 362.237 CPU sec, 11.768 real sec
[M::mem_process_seqs] Processed 675216 reads in 254.561 CPU sec, 8.200 real sec
[M::mem_process_seqs] Processed 675290 reads in 265.453 CPU sec, 8.573 real sec
sorting block 9, #reads = 1080614 mem = 536878862 peak = 1043800064
[M::process] read 675222 sequences (100000026 bp) in 2.408 CPU sec, 2.571 real sec...
sorting block 10, #reads = 1080828 mem = 536879270 peak = 1058680832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285678, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (251.37, 125.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675230 sequences (100000082 bp) in 2.077 CPU sec, 2.593 real sec...
[M::process] read 675070 sequences (100000085 bp) in 1.831 CPU sec, 1.867 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285925, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.27, 127.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675152 reads in 254.258 CPU sec, 8.329 real sec
[M::mem_process_seqs] Processed 675222 reads in 143.200 CPU sec, 4.659 real sec
sorting block 11, #reads = 1080319 mem = 536880937 peak = 1058680832
[M::process] read 675142 sequences (100000243 bp) in 2.317 CPU sec, 2.894 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285868, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.90, 126.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675084 sequences (100000083 bp) in 1.802 CPU sec, 1.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285097, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.98, 125.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675230 reads in 248.510 CPU sec, 8.085 real sec
[M::mem_process_seqs] Processed 675070 reads in 141.115 CPU sec, 4.520 real sec
sorting block 12, #reads = 1080761 mem = 536876259 peak = 1058680832
[M::process] read 675178 sequences (100000097 bp) in 2.035 CPU sec, 2.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285464, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.44, 127.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 18, 128)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348)
[M::mem_pestat] mean and std.dev: (25.08, 32.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 458)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675034 sequences (100000071 bp) in 1.814 CPU sec, 1.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285913, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.38, 128.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285541, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.51, 127.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675142 reads in 364.315 CPU sec, 11.818 real sec
sorting block 13, #reads = 1080352 mem = 536878403 peak = 1078251520
[M::mem_process_seqs] Processed 675084 reads in 255.252 CPU sec, 8.187 real sec
[M::mem_process_seqs] Processed 675178 reads in 266.264 CPU sec, 8.565 real sec
sorting block 14, #reads = 1081097 mem = 536879807 peak = 1086615552
[M::process] read 674950 sequences (100000260 bp) in 2.201 CPU sec, 2.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285549, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.67, 126.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 1522, 3338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9970)
[M::mem_pestat] mean and std.dev: (1599.64, 1551.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13286)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675064 sequences (100000208 bp) in 2.187 CPU sec, 2.915 real sec...
[M::process] read 675012 sequences (100000175 bp) in 1.762 CPU sec, 1.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285774, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (255.04, 128.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 183, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498)
[M::mem_pestat] mean and std.dev: (145.89, 109.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 603)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675034 reads in 255.177 CPU sec, 8.323 real sec
[M::mem_process_seqs] Processed 674950 reads in 143.765 CPU sec, 4.630 real sec
sorting block 15, #reads = 1080750 mem = 536882507 peak = 1111527424
[M::process] read 674982 sequences (100000138 bp) in 2.110 CPU sec, 2.807 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285527, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (254.20, 127.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 45, 58)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 152)
[M::mem_pestat] mean and std.dev: (32.60, 23.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 199)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 16, #reads = 1080867 mem = 536879211 peak = 1119137792
[M::process] read 675022 sequences (100000204 bp) in 1.764 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286263, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.65, 128.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675064 reads in 248.975 CPU sec, 8.168 real sec
[M::mem_process_seqs] Processed 675012 reads in 138.839 CPU sec, 4.512 real sec
sorting block 17, #reads = 1080951 mem = 536880957 peak = 1119137792
[M::process] read 674840 sequences (100000288 bp) in 2.355 CPU sec, 2.648 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285048, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.79, 128.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 24, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 178)
[M::mem_pestat] mean and std.dev: (30.38, 31.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 232)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674966 sequences (100000067 bp) in 1.801 CPU sec, 1.906 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284923, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.88, 129.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285651, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.26, 130.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 298, 614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1778)
[M::mem_pestat] mean and std.dev: (302.92, 255.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2360)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674982 reads in 365.909 CPU sec, 11.898 real sec
[M::mem_process_seqs] Processed 675022 reads in 256.515 CPU sec, 8.294 real sec
[M::mem_process_seqs] Processed 674840 reads in 267.700 CPU sec, 8.673 real sec
sorting block 18, #reads = 1080296 mem = 536880217 peak = 1119137792
[M::process] read 674764 sequences (100000255 bp) in 2.401 CPU sec, 2.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285613, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.36, 131.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (360, 360, 576)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (426.64, 216.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 19, #reads = 1080503 mem = 536878568 peak = 1135198208
[M::process] read 674238 sequences (100000286 bp) in 2.298 CPU sec, 2.721 real sec...
[M::process] read 674056 sequences (100000279 bp) in 1.846 CPU sec, 2.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285493, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.76, 136.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674966 reads in 258.261 CPU sec, 8.453 real sec
[M::mem_process_seqs] Processed 674764 reads in 145.825 CPU sec, 4.747 real sec
sorting block 20, #reads = 1080308 mem = 536877990 peak = 1135198208
[M::process] read 674462 sequences (100000021 bp) in 2.271 CPU sec, 2.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284476, 24, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.68, 145.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 26, 42)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 96)
[M::mem_pestat] mean and std.dev: (29.86, 20.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 123)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674872 sequences (100000033 bp) in 1.825 CPU sec, 1.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285347, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.71, 138.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284367, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 199, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (278.94, 147.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 73, 82)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (59.53, 28.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 190)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674238 reads in 380.385 CPU sec, 12.343 real sec
[M::mem_process_seqs] Processed 674462 reads in 264.048 CPU sec, 8.505 real sec
[M::mem_process_seqs] Processed 674056 reads in 275.725 CPU sec, 8.903 real sec
sorting block 21, #reads = 1080020 mem = 536879861 peak = 1135198208
[M::process] read 674754 sequences (100000185 bp) in 2.422 CPU sec, 2.594 real sec...
sorting block 22, #reads = 1079676 mem = 536878239 peak = 1144135680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285785, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.60, 132.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 704, 8627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25453)
[M::mem_pestat] mean and std.dev: (2760.73, 3662.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 33866)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674386 sequences (100000051 bp) in 1.931 CPU sec, 2.498 real sec...
[M::process] read 674102 sequences (100000110 bp) in 1.783 CPU sec, 1.893 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285427, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.68, 135.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674872 reads in 253.216 CPU sec, 8.299 real sec
[M::mem_process_seqs] Processed 674754 reads in 144.331 CPU sec, 4.719 real sec
sorting block 23, #reads = 1079901 mem = 536879520 peak = 1144135680
[M::process] read 674090 sequences (100000136 bp) in 2.301 CPU sec, 3.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285384, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.65, 135.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674182 sequences (100000084 bp) in 1.799 CPU sec, 1.824 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284744, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 199, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (280.42, 150.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674386 reads in 257.343 CPU sec, 8.427 real sec
[M::mem_process_seqs] Processed 674102 reads in 144.566 CPU sec, 4.632 real sec
sorting block 24, #reads = 1080325 mem = 536879557 peak = 1144135680
[M::process] read 674482 sequences (100000269 bp) in 2.221 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284537, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (277.72, 148.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 73, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (76.12, 98.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674604 sequences (100000051 bp) in 1.778 CPU sec, 1.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285177, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.87, 136.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 644, 783)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2147)
[M::mem_pestat] mean and std.dev: (414.50, 331.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2829)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284811, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.06, 146.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674090 reads in 370.233 CPU sec, 12.011 real sec
[M::mem_process_seqs] Processed 674482 reads in 256.069 CPU sec, 8.228 real sec
sorting block 25, #reads = 1080647 mem = 536881215 peak = 1145520128
[M::mem_process_seqs] Processed 674182 reads in 267.458 CPU sec, 8.620 real sec
sorting block 26, #reads = 1079837 mem = 536878593 peak = 1152364544
[M::process] read 674680 sequences (100000282 bp) in 2.518 CPU sec, 2.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285152, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.81, 137.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674488 sequences (100000233 bp) in 2.044 CPU sec, 2.696 real sec...
[M::process] read 675336 sequences (100000164 bp) in 1.855 CPU sec, 1.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285227, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.34, 137.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 14, 801)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2397)
[M::mem_pestat] mean and std.dev: (221.85, 330.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3195)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674604 reads in 255.909 CPU sec, 8.483 real sec
[M::mem_process_seqs] Processed 674680 reads in 143.346 CPU sec, 4.617 real sec
sorting block 27, #reads = 1079936 mem = 536879484 peak = 1152364544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285818, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.12, 137.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675306 sequences (100000122 bp) in 2.236 CPU sec, 2.953 real sec...
sorting block 28, #reads = 1079530 mem = 536880756 peak = 1154125824
[M::process] read 675132 sequences (100000253 bp) in 1.808 CPU sec, 2.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286306, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.41, 125.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674488 reads in 238.791 CPU sec, 7.838 real sec
[M::mem_process_seqs] Processed 675336 reads in 137.118 CPU sec, 4.453 real sec
sorting block 29, #reads = 1079590 mem = 536877011 peak = 1154125824
[M::process] read 675162 sequences (100000161 bp) in 2.199 CPU sec, 2.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286071, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.67, 127.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675154 sequences (100000148 bp) in 1.837 CPU sec, 1.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285821, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.37, 128.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285251, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.22, 129.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 25, 1121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3357)
[M::mem_pestat] mean and std.dev: (151.82, 359.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4475)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675306 reads in 355.683 CPU sec, 11.564 real sec
[M::mem_process_seqs] Processed 675132 reads in 249.338 CPU sec, 8.064 real sec
[M::mem_process_seqs] Processed 675162 reads in 260.563 CPU sec, 8.450 real sec
sorting block 30, #reads = 1080866 mem = 536878042 peak = 1154125824
[M::process] read 674934 sequences (100000257 bp) in 2.393 CPU sec, 2.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285935, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.85, 129.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1080386 mem = 536881851 peak = 1169272832
[M::process] read 675032 sequences (100000237 bp) in 2.309 CPU sec, 2.730 real sec...
[M::process] read 675030 sequences (100000189 bp) in 1.790 CPU sec, 1.938 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285678, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.03, 129.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675154 reads in 250.326 CPU sec, 8.207 real sec
[M::mem_process_seqs] Processed 674934 reads in 141.375 CPU sec, 4.595 real sec
sorting block 32, #reads = 1080543 mem = 536880987 peak = 1169272832
[M::process] read 675012 sequences (100000056 bp) in 2.307 CPU sec, 2.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285199, 24, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.46, 130.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (155.26, 129.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1339)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675050 sequences (100000235 bp) in 1.780 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285632, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.76, 129.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (674, 1691, 1691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3725)
[M::mem_pestat] mean and std.dev: (947.11, 729.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4742)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675032 reads in 246.721 CPU sec, 8.025 real sec
[M::mem_process_seqs] Processed 675030 reads in 139.552 CPU sec, 4.492 real sec
sorting block 33, #reads = 1080472 mem = 536879212 peak = 1169272832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286560, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.88, 130.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674974 sequences (100000111 bp) in 2.234 CPU sec, 3.027 real sec...
[M::process] read 674858 sequences (100000094 bp) in 1.813 CPU sec, 1.849 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285738, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.02, 130.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675012 reads in 243.261 CPU sec, 7.932 real sec
[M::mem_process_seqs] Processed 675050 reads in 139.596 CPU sec, 4.490 real sec
sorting block 34, #reads = 1080577 mem = 536876145 peak = 1169272832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285987, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (257.17, 130.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675232 sequences (100000007 bp) in 2.407 CPU sec, 2.964 real sec...
[M::process] read 675270 sequences (100000201 bp) in 1.784 CPU sec, 1.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285954, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.56, 130.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674974 reads in 240.147 CPU sec, 7.812 real sec
sorting block 35, #reads = 1080244 mem = 536876635 peak = 1185042432
[M::mem_process_seqs] Processed 674858 reads in 137.282 CPU sec, 4.401 real sec
sorting block 36, #reads = 1080130 mem = 536878171 peak = 1192972288
[M::process] read 675316 sequences (100000001 bp) in 2.379 CPU sec, 3.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285442, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.10, 126.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675226 sequences (100000045 bp) in 1.826 CPU sec, 1.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285391, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.88, 125.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 87, 169)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443)
[M::mem_pestat] mean and std.dev: (91.00, 68.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 580)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675232 reads in 252.583 CPU sec, 8.323 real sec
[M::mem_process_seqs] Processed 675270 reads in 146.480 CPU sec, 4.799 real sec
sorting block 37, #reads = 1080517 mem = 536880184 peak = 1192972288
[M::process] read 675392 sequences (100000172 bp) in 2.292 CPU sec, 2.885 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285669, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.26, 124.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675204 sequences (100000051 bp) in 1.791 CPU sec, 1.844 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285962, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.85, 124.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285112, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.93, 125.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675316 reads in 365.251 CPU sec, 11.874 real sec
[M::mem_process_seqs] Processed 675226 reads in 255.176 CPU sec, 8.241 real sec
[M::mem_process_seqs] Processed 675392 reads in 266.044 CPU sec, 8.616 real sec
sorting block 38, #reads = 1080936 mem = 536876377 peak = 1192972288
[M::process] read 675248 sequences (100000221 bp) in 2.550 CPU sec, 2.726 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285660, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 39, #reads = 1080417 mem = 536878970 peak = 1194377216
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.59, 125.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675124 sequences (100000134 bp) in 2.149 CPU sec, 2.738 real sec...
[M::process] read 675208 sequences (100000081 bp) in 1.853 CPU sec, 1.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285985, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.54, 127.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675204 reads in 253.574 CPU sec, 8.292 real sec
[M::mem_process_seqs] Processed 675248 reads in 141.949 CPU sec, 4.620 real sec
sorting block 40, #reads = 1080435 mem = 536878663 peak = 1194377216
[M::process] read 675272 sequences (100000105 bp) in 2.249 CPU sec, 2.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285431, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.73, 127.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675118 sequences (100000113 bp) in 1.839 CPU sec, 1.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285464, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.48, 126.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675124 reads in 250.900 CPU sec, 8.170 real sec
[M::mem_process_seqs] Processed 675208 reads in 142.343 CPU sec, 4.570 real sec
sorting block 41, #reads = 1080880 mem = 536880916 peak = 1194377216
[M::process] read 675228 sequences (100000254 bp) in 2.177 CPU sec, 2.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285635, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.20, 127.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 17, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278)
[M::mem_pestat] mean and std.dev: (36.00, 34.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 365)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675222 sequences (100000228 bp) in 1.930 CPU sec, 1.972 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285604, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.08, 130.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675272 reads in 251.791 CPU sec, 8.198 real sec
[M::mem_process_seqs] Processed 675118 reads in 141.308 CPU sec, 4.518 real sec
sorting block 42, #reads = 1080594 mem = 536878945 peak = 1209606144
sorting block 43, #reads = 1080729 mem = 536878577 peak = 1209606144
[M::process] read 675200 sequences (100000086 bp) in 2.137 CPU sec, 2.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285749, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.97, 129.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 359, 2292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6862)
[M::mem_pestat] mean and std.dev: (762.70, 1009.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9147)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675014 sequences (100000150 bp) in 1.871 CPU sec, 2.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285609, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.59, 129.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 98, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (132.50, 157.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675228 reads in 249.421 CPU sec, 8.174 real sec
[M::mem_process_seqs] Processed 675222 reads in 139.973 CPU sec, 4.544 real sec
sorting block 44, #reads = 1081396 mem = 536876684 peak = 1209606144
[M::process] read 675096 sequences (100000128 bp) in 2.292 CPU sec, 2.743 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285563, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.34, 129.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 373, 1414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4224)
[M::mem_pestat] mean and std.dev: (651.50, 716.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5629)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675126 sequences (100000060 bp) in 1.968 CPU sec, 2.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286007, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (255.00, 128.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285658, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.48, 128.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675200 reads in 366.049 CPU sec, 11.893 real sec
[M::mem_process_seqs] Processed 675014 reads in 257.082 CPU sec, 8.299 real sec
[M::mem_process_seqs] Processed 675096 reads in 267.886 CPU sec, 8.667 real sec
sorting block 45, #reads = 1080045 mem = 536877549 peak = 1209606144
[M::process] read 674984 sequences (100000278 bp) in 2.336 CPU sec, 2.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285759, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.56, 129.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1080669 mem = 536876017 peak = 1217249280
[M::process] read 674956 sequences (100000179 bp) in 2.448 CPU sec, 2.630 real sec...
[M::process] read 674876 sequences (100000270 bp) in 1.850 CPU sec, 1.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285878, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.72, 129.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675126 reads in 257.442 CPU sec, 8.428 real sec
[M::mem_process_seqs] Processed 674984 reads in 144.427 CPU sec, 4.696 real sec
sorting block 47, #reads = 1081069 mem = 536879535 peak = 1217249280
[M::process] read 674852 sequences (100000220 bp) in 2.508 CPU sec, 3.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285069, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (256.15, 129.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674826 sequences (100000231 bp) in 1.841 CPU sec, 1.860 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285169, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (258.01, 131.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674956 reads in 248.663 CPU sec, 8.081 real sec
[M::mem_process_seqs] Processed 674876 reads in 139.567 CPU sec, 4.483 real sec
sorting block 48, #reads = 1080414 mem = 536878274 peak = 1217249280
[M::process] read 674206 sequences (100000188 bp) in 2.404 CPU sec, 2.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285558, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.73, 132.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 176, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (236.81, 230.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1374)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674092 sequences (100000179 bp) in 1.838 CPU sec, 1.928 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285299, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.72, 134.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674852 reads in 253.036 CPU sec, 8.238 real sec
[M::mem_process_seqs] Processed 674826 reads in 142.550 CPU sec, 4.562 real sec
sorting block 49, #reads = 1080265 mem = 536879629 peak = 1225392128
sorting block 50, #reads = 1081060 mem = 536879240 peak = 1225392128
[M::process] read 674520 sequences (100000190 bp) in 2.482 CPU sec, 3.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284532, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (276.34, 146.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674788 sequences (100000226 bp) in 1.931 CPU sec, 2.078 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284965, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 200, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (281.73, 150.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (357, 445, 658)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260)
[M::mem_pestat] mean and std.dev: (432.54, 216.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1561)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674206 reads in 258.599 CPU sec, 8.469 real sec
[M::mem_process_seqs] Processed 674092 reads in 144.402 CPU sec, 4.698 real sec
sorting block 51, #reads = 1079537 mem = 536877879 peak = 1225392128
[M::process] read 674842 sequences (100000153 bp) in 2.480 CPU sec, 3.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285173, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (268.40, 140.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (444, 469, 2196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5700)
[M::mem_pestat] mean and std.dev: (896.33, 950.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7452)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674566 sequences (100000268 bp) in 1.978 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285523, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.55, 133.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285472, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.47, 133.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 546, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1396)
[M::mem_pestat] mean and std.dev: (420.00, 289.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1821)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674520 reads in 370.266 CPU sec, 12.016 real sec
[M::mem_process_seqs] Processed 674842 reads in 257.489 CPU sec, 8.309 real sec
[M::mem_process_seqs] Processed 674788 reads in 268.592 CPU sec, 8.690 real sec
sorting block 52, #reads = 1079576 mem = 536880577 peak = 1225392128
[M::process] read 674352 sequences (100000219 bp) in 2.751 CPU sec, 3.023 real sec...
sorting block 53, #reads = 1080458 mem = 536878724 peak = 1225392128
[M::process] read 674042 sequences (100000141 bp) in 2.484 CPU sec, 2.860 real sec...
[M::process] read 674322 sequences (100000268 bp) in 1.833 CPU sec, 1.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284905, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.72, 144.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674352 reads in 139.080 CPU sec, 4.527 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285294, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.48, 135.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (67, 340, 666)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1864)
[M::mem_pestat] mean and std.dev: (340.00, 269.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2463)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674542 sequences (100000255 bp) in 2.140 CPU sec, 2.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284823, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (278.80, 149.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674566 reads in 389.060 CPU sec, 12.656 real sec
[M::mem_process_seqs] Processed 674042 reads in 157.583 CPU sec, 5.066 real sec
sorting block 54, #reads = 1079694 mem = 536880184 peak = 1225392128
sorting block 55, #reads = 1079826 mem = 536882423 peak = 1227010048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285604, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674512 sequences (100000200 bp) in 2.474 CPU sec, 3.060 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.40, 143.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674610 sequences (100000120 bp) in 2.039 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284801, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.66, 136.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674322 reads in 251.053 CPU sec, 8.229 real sec
[M::mem_process_seqs] Processed 674542 reads in 139.664 CPU sec, 4.541 real sec
sorting block 56, #reads = 1079903 mem = 536880531 peak = 1227010048
[M::process] read 674444 sequences (100000011 bp) in 2.339 CPU sec, 2.999 real sec...
[M::process] read 675274 sequences (100000297 bp) in 1.952 CPU sec, 1.962 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285493, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.85, 139.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (626, 652, 776)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (326, 1076)
[M::mem_pestat] mean and std.dev: (687.47, 174.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1384)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674610 reads in 126.413 CPU sec, 4.083 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285248, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.61, 138.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 57, #reads = 1080046 mem = 536881365 peak = 1227010048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286222, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.12, 138.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675148 sequences (100000109 bp) in 2.088 CPU sec, 2.548 real sec...
[M::mem_process_seqs] Processed 674512 reads in 366.851 CPU sec, 11.952 real sec
[M::mem_process_seqs] Processed 674444 reads in 148.644 CPU sec, 4.852 real sec
sorting block 58, #reads = 1079569 mem = 536880376 peak = 1227010048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286344, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.11, 126.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 15, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (74.00, 153.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675094 sequences (100000006 bp) in 2.654 CPU sec, 2.797 real sec...
[M::process] read 675092 sequences (100000218 bp) in 1.890 CPU sec, 2.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286399, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.35, 127.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675274 reads in 234.488 CPU sec, 7.644 real sec
[M::mem_process_seqs] Processed 675148 reads in 133.235 CPU sec, 4.336 real sec
sorting block 59, #reads = 1080573 mem = 536879754 peak = 1227010048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286208, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.72, 127.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675102 sequences (100000188 bp) in 2.246 CPU sec, 2.775 real sec...
[M::process] read 675016 sequences (100000283 bp) in 1.832 CPU sec, 2.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285785, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.31, 129.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 759, 1314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3908)
[M::mem_pestat] mean and std.dev: (636.60, 595.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5205)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675094 reads in 234.322 CPU sec, 7.625 real sec
[M::mem_process_seqs] Processed 675092 reads in 134.212 CPU sec, 4.312 real sec
sorting block 60, #reads = 1080731 mem = 536878548 peak = 1227010048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285926, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.28, 130.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674998 sequences (100000023 bp) in 2.266 CPU sec, 3.260 real sec...
[M::process] read 674816 sequences (100000224 bp) in 1.957 CPU sec, 2.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286090, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.42, 130.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675102 reads in 237.061 CPU sec, 7.712 real sec
[M::mem_process_seqs] Processed 675016 reads in 134.596 CPU sec, 4.296 real sec
sorting block 61, #reads = 1079948 mem = 536880271 peak = 1235959808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285655, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.58, 131.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 26, 128)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352)
[M::mem_pestat] mean and std.dev: (70.29, 73.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 464)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674840 sequences (100000148 bp) in 2.432 CPU sec, 3.071 real sec...
sorting block 62, #reads = 1080917 mem = 536880076 peak = 1236566016
[M::process] read 674786 sequences (100000280 bp) in 1.834 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285675, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.62, 131.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674998 reads in 240.577 CPU sec, 7.881 real sec
[M::mem_process_seqs] Processed 674816 reads in 136.315 CPU sec, 4.423 real sec
sorting block 63, #reads = 1080454 mem = 536877713 peak = 1236566016
[M::process] read 674738 sequences (100000258 bp) in 2.543 CPU sec, 2.757 real sec...
[M::process] read 674796 sequences (100000080 bp) in 1.888 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286159, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (258.75, 131.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674786 reads in 125.109 CPU sec, 4.039 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286324, 17, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.37, 131.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (104, 104, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488)
[M::mem_pestat] mean and std.dev: (135.29, 77.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 616)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674848 sequences (100000124 bp) in 1.874 CPU sec, 1.896 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285688, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.20, 132.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674840 reads in 363.078 CPU sec, 11.756 real sec
sorting block 64, #reads = 1079490 mem = 536876999 peak = 1236566016
[M::mem_process_seqs] Processed 674738 reads in 149.409 CPU sec, 4.815 real sec
sorting block 65, #reads = 1080321 mem = 536877416 peak = 1244934144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285537, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.29, 132.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675264 sequences (100000116 bp) in 2.691 CPU sec, 3.208 real sec...
[M::process] read 675370 sequences (100000076 bp) in 1.878 CPU sec, 1.966 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285588, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.19, 132.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 17, 1034)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3096)
[M::mem_pestat] mean and std.dev: (307.00, 445.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4127)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674796 reads in 246.105 CPU sec, 8.073 real sec
[M::process] read 675206 sequences (100000116 bp) in 1.922 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286054, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.31, 126.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675264 reads in 130.841 CPU sec, 4.286 real sec
sorting block 66, #reads = 1080256 mem = 536879254 peak = 1244934144
[M::mem_process_seqs] Processed 674848 reads in 317.385 CPU sec, 10.384 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286005, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.69, 126.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1080498 mem = 536878660 peak = 1245282304
[M::process] read 675158 sequences (100000116 bp) in 2.532 CPU sec, 2.999 real sec...
[M::process] read 675144 sequences (100000091 bp) in 1.920 CPU sec, 2.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286672, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.47, 126.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 44, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1433)
[M::mem_pestat] mean and std.dev: (182.73, 237.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1896)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675370 reads in 255.443 CPU sec, 8.366 real sec
[M::mem_process_seqs] Processed 675206 reads in 138.252 CPU sec, 4.502 real sec
sorting block 68, #reads = 1080745 mem = 536879027 peak = 1245282304
[M::process] read 675116 sequences (100000019 bp) in 2.415 CPU sec, 2.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285498, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.57, 125.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675102 sequences (100000293 bp) in 1.918 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285999, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.76, 127.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285882, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.62, 125.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675158 reads in 357.805 CPU sec, 11.630 real sec
[M::mem_process_seqs] Processed 675116 reads in 251.258 CPU sec, 8.108 real sec
[M::mem_process_seqs] Processed 675144 reads in 262.336 CPU sec, 8.484 real sec
sorting block 69, #reads = 1080491 mem = 536877840 peak = 1245282304
[M::process] read 675186 sequences (100000038 bp) in 2.688 CPU sec, 2.926 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285770, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.17, 127.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 25, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219)
[M::mem_pestat] mean and std.dev: (163.69, 194.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1624)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 70, #reads = 1080900 mem = 536880503 peak = 1245282304
[M::process] read 674942 sequences (100000081 bp) in 2.361 CPU sec, 2.769 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286002, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.99, 128.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675074 sequences (100000147 bp) in 1.852 CPU sec, 1.969 real sec...
[M::mem_process_seqs] Processed 675102 reads in 251.598 CPU sec, 8.248 real sec
[M::mem_process_seqs] Processed 675186 reads in 141.379 CPU sec, 4.600 real sec
sorting block 71, #reads = 1080603 mem = 536880412 peak = 1245282304
[M::process] read 675128 sequences (100000048 bp) in 2.382 CPU sec, 2.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285532, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.34, 129.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 26, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1234)
[M::mem_pestat] mean and std.dev: (133.73, 192.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1642)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675022 sequences (100000133 bp) in 1.999 CPU sec, 2.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285959, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.35, 129.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6699, 6699, 6699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (6699, 6699)
[M::mem_pestat] mean and std.dev: (6699.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (6699, 6699)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674942 reads in 245.894 CPU sec, 8.042 real sec
[M::mem_process_seqs] Processed 675074 reads in 137.733 CPU sec, 4.417 real sec
sorting block 72, #reads = 1080825 mem = 536879201 peak = 1245282304
[M::process] read 675058 sequences (100000287 bp) in 2.182 CPU sec, 2.933 real sec...
[M::process] read 675030 sequences (100000278 bp) in 1.994 CPU sec, 2.037 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285666, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.92, 130.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 1419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4251)
[M::mem_pestat] mean and std.dev: (289.27, 537.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5667)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675022 reads in 127.097 CPU sec, 4.047 real sec
sorting block 73, #reads = 1080672 mem = 536879336 peak = 1246457856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285914, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.97, 131.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 674, 1254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3328)
[M::mem_pestat] mean and std.dev: (590.17, 455.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4365)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286344, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.50, 131.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674998 sequences (100000096 bp) in 2.075 CPU sec, 2.317 real sec...
[M::mem_process_seqs] Processed 675128 reads in 367.600 CPU sec, 12.000 real sec
[M::mem_process_seqs] Processed 675058 reads in 147.369 CPU sec, 4.825 real sec
sorting block 74, #reads = 1080514 mem = 536880616 peak = 1246457856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286020, 14, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.58, 131.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 32, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142)
[M::mem_pestat] mean and std.dev: (200.43, 294.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1516)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674940 sequences (100000103 bp) in 2.306 CPU sec, 3.175 real sec...
[M::process] read 675008 sequences (100000093 bp) in 1.905 CPU sec, 1.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286250, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.10, 131.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 382, 2945)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8815)
[M::mem_pestat] mean and std.dev: (854.17, 1222.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11750)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675030 reads in 242.498 CPU sec, 7.893 real sec
[M::mem_process_seqs] Processed 674998 reads in 136.012 CPU sec, 4.357 real sec
sorting block 75, #reads = 1080713 mem = 536879009 peak = 1246457856
[M::process] read 674972 sequences (100000159 bp) in 2.218 CPU sec, 2.858 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286306, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.32, 131.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674860 sequences (100000013 bp) in 1.977 CPU sec, 2.019 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285850, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (256.25, 130.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674940 reads in 248.084 CPU sec, 8.071 real sec
sorting block 76, #reads = 1080578 mem = 536877347 peak = 1246457856
[M::mem_process_seqs] Processed 675008 reads in 141.519 CPU sec, 4.524 real sec
sorting block 77, #reads = 1080428 mem = 536880412 peak = 1246457856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286161, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.58, 130.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 58, 1878)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5518)
[M::mem_pestat] mean and std.dev: (562.31, 754.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7338)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674858 sequences (100000253 bp) in 2.295 CPU sec, 3.035 real sec...
[M::process] read 674794 sequences (100000095 bp) in 1.952 CPU sec, 2.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285899, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.40, 130.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674972 reads in 247.122 CPU sec, 8.101 real sec
[M::mem_process_seqs] Processed 674860 reads in 138.619 CPU sec, 4.499 real sec
sorting block 78, #reads = 1080966 mem = 536878542 peak = 1246457856
[M::process] read 674650 sequences (100000051 bp) in 2.517 CPU sec, 3.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285709, 26, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.10, 132.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 578, 1042)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2778)
[M::mem_pestat] mean and std.dev: (558.19, 440.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3646)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674702 sequences (100000186 bp) in 1.976 CPU sec, 2.056 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285547, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.49, 133.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674858 reads in 245.483 CPU sec, 8.005 real sec
[M::mem_process_seqs] Processed 674794 reads in 138.366 CPU sec, 4.473 real sec
sorting block 79, #reads = 1080674 mem = 536878632 peak = 1246457856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285400, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.58, 133.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674776 sequences (100000200 bp) in 2.207 CPU sec, 3.127 real sec...
[M::process] read 674744 sequences (100000206 bp) in 1.949 CPU sec, 1.950 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285633, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.92, 135.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674650 reads in 248.792 CPU sec, 8.081 real sec
[M::mem_process_seqs] Processed 674702 reads in 139.782 CPU sec, 4.458 real sec
sorting block 80, #reads = 1080461 mem = 536878792 peak = 1248456704
[M::process] read 674764 sequences (100000024 bp) in 2.489 CPU sec, 2.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285424, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.91, 134.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674706 sequences (100000040 bp) in 1.876 CPU sec, 1.900 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285765, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.63, 134.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 62, 636)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1856)
[M::mem_pestat] mean and std.dev: (234.00, 288.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2466)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674776 reads in 246.552 CPU sec, 8.002 real sec
sorting block 81, #reads = 1080161 mem = 536878722 peak = 1255890944
[M::mem_process_seqs] Processed 674744 reads in 139.639 CPU sec, 4.452 real sec
sorting block 82, #reads = 1080212 mem = 536877666 peak = 1255890944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285112, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674678 sequences (100000152 bp) in 2.309 CPU sec, 2.956 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.91, 134.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 128, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (201.26, 152.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674550 sequences (100000064 bp) in 1.851 CPU sec, 1.953 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285827, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.21, 136.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 61, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (61, 61)
[M::mem_pestat] mean and std.dev: (61.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (61, 61)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674764 reads in 250.895 CPU sec, 8.221 real sec
[M::mem_process_seqs] Processed 674706 reads in 139.264 CPU sec, 4.519 real sec
sorting block 83, #reads = 1079846 mem = 536880403 peak = 1255890944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286258, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.33, 134.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 290, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515)
[M::mem_pestat] mean and std.dev: (203.73, 108.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674634 sequences (100000212 bp) in 2.248 CPU sec, 2.988 real sec...
[M::process] read 674618 sequences (100000228 bp) in 1.973 CPU sec, 1.980 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286116, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.25, 137.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674678 reads in 242.691 CPU sec, 7.905 real sec
[M::mem_process_seqs] Processed 674550 reads in 135.981 CPU sec, 4.372 real sec
sorting block 84, #reads = 1080360 mem = 536878156 peak = 1255890944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286259, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.47, 137.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674542 sequences (100000282 bp) in 2.294 CPU sec, 2.841 real sec...
[M::process] read 674538 sequences (100000073 bp) in 1.919 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285452, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.87, 137.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 1488, 1488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4410)
[M::mem_pestat] mean and std.dev: (803.10, 691.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5871)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674634 reads in 241.732 CPU sec, 7.857 real sec
[M::mem_process_seqs] Processed 674618 reads in 137.415 CPU sec, 4.391 real sec
sorting block 85, #reads = 1079937 mem = 536878673 peak = 1255890944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285304, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.48, 138.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674572 sequences (100000247 bp) in 2.585 CPU sec, 3.070 real sec...
sorting block 86, #reads = 1080341 mem = 536877405 peak = 1256144896
[M::process] read 674908 sequences (100000278 bp) in 1.877 CPU sec, 2.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285548, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.25, 139.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674542 reads in 239.180 CPU sec, 7.814 real sec
[M::mem_process_seqs] Processed 674538 reads in 135.652 CPU sec, 4.400 real sec
sorting block 87, #reads = 1079614 mem = 536879179 peak = 1256144896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286062, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.53, 138.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675216 sequences (100000278 bp) in 2.302 CPU sec, 2.822 real sec...
[M::process] read 675134 sequences (100000002 bp) in 1.869 CPU sec, 2.111 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286516, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.67, 129.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674572 reads in 230.173 CPU sec, 7.511 real sec
[M::mem_process_seqs] Processed 674908 reads in 129.883 CPU sec, 4.220 real sec
sorting block 88, #reads = 1080342 mem = 536878534 peak = 1256144896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286402, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (252.32, 125.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675104 sequences (100000254 bp) in 2.363 CPU sec, 2.785 real sec...
[M::process] read 674944 sequences (100000210 bp) in 1.938 CPU sec, 2.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286212, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.89, 127.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 88, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1654)
[M::mem_pestat] mean and std.dev: (201.27, 238.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2205)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675216 reads in 233.099 CPU sec, 7.600 real sec
[M::mem_process_seqs] Processed 675134 reads in 132.902 CPU sec, 4.277 real sec
sorting block 89, #reads = 1080424 mem = 536878600 peak = 1256144896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286303, 27, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.51, 128.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 32, 2500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7484)
[M::mem_pestat] mean and std.dev: (850.30, 1166.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9976)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675046 sequences (100000218 bp) in 2.313 CPU sec, 2.961 real sec...
[M::process] read 674990 sequences (100000046 bp) in 1.880 CPU sec, 1.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286399, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.85, 129.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675104 reads in 235.285 CPU sec, 7.658 real sec
[M::mem_process_seqs] Processed 674944 reads in 133.024 CPU sec, 4.245 real sec
sorting block 90, #reads = 1080452 mem = 536880608 peak = 1263595520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286233, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.42, 128.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 158, 704)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080)
[M::mem_pestat] mean and std.dev: (307.38, 338.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2768)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 91, #reads = 1080198 mem = 536877272 peak = 1263595520
[M::process] read 674782 sequences (100000004 bp) in 2.449 CPU sec, 3.154 real sec...
[M::process] read 674836 sequences (100000215 bp) in 1.852 CPU sec, 1.877 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285904, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (256.85, 129.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675046 reads in 238.381 CPU sec, 7.819 real sec
[M::mem_process_seqs] Processed 674990 reads in 136.648 CPU sec, 4.448 real sec
sorting block 92, #reads = 1080547 mem = 536877846 peak = 1263595520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285639, 17, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.52, 129.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1227)
[M::mem_pestat] mean and std.dev: (58.13, 138.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1635)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674946 sequences (100000021 bp) in 2.369 CPU sec, 2.838 real sec...
[M::process] read 674668 sequences (100000066 bp) in 1.964 CPU sec, 2.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286024, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.57, 130.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674782 reads in 240.679 CPU sec, 7.848 real sec
[M::mem_process_seqs] Processed 674836 reads in 136.625 CPU sec, 4.430 real sec
sorting block 93, #reads = 1079947 mem = 536877789 peak = 1263595520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285764, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.46, 129.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 10, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304)
[M::mem_pestat] mean and std.dev: (25.75, 40.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 404)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674776 sequences (100000090 bp) in 2.499 CPU sec, 3.025 real sec...
[M::process] read 674674 sequences (100000288 bp) in 1.978 CPU sec, 2.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285903, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.64, 130.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674946 reads in 239.007 CPU sec, 7.771 real sec
[M::mem_process_seqs] Processed 674668 reads in 135.924 CPU sec, 4.359 real sec
sorting block 94, #reads = 1080114 mem = 536878852 peak = 1263595520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286289, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.02, 133.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 9556, 9556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28234)
[M::mem_pestat] mean and std.dev: (4941.39, 4618.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 37573)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674708 sequences (100000261 bp) in 2.362 CPU sec, 3.059 real sec...
[M::process] read 675096 sequences (100000259 bp) in 1.905 CPU sec, 1.911 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285863, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.11, 134.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674776 reads in 237.809 CPU sec, 7.736 real sec
sorting block 95, #reads = 1080553 mem = 536879829 peak = 1271189504
[M::process] read 675200 sequences (100000014 bp) in 2.115 CPU sec, 2.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285454, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.88, 132.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 25, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308)
[M::mem_pestat] mean and std.dev: (35.12, 40.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 410)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674708 reads in 127.876 CPU sec, 4.169 real sec
sorting block 96, #reads = 1080473 mem = 536878813 peak = 1271189504
[M::mem_process_seqs] Processed 674674 reads in 314.926 CPU sec, 10.189 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286272, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.23, 125.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675128 sequences (100000288 bp) in 2.306 CPU sec, 3.112 real sec...
[M::process] read 675338 sequences (100000146 bp) in 2.000 CPU sec, 2.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286074, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.18, 124.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 9, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (76.27, 110.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 861)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675096 reads in 251.264 CPU sec, 8.143 real sec
[M::mem_process_seqs] Processed 675200 reads in 138.075 CPU sec, 4.414 real sec
sorting block 97, #reads = 1080223 mem = 536879071 peak = 1271283712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285970, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.36, 125.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 106, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (137.13, 145.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675252 sequences (100000230 bp) in 2.396 CPU sec, 3.054 real sec...
sorting block 98, #reads = 1080757 mem = 536879817 peak = 1271685120
[M::process] read 675048 sequences (100000171 bp) in 1.989 CPU sec, 2.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286105, 14, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.87, 125.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 97, 1304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3886)
[M::mem_pestat] mean and std.dev: (426.23, 536.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5177)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675128 reads in 244.209 CPU sec, 7.993 real sec
[M::mem_process_seqs] Processed 675338 reads in 139.076 CPU sec, 4.504 real sec
sorting block 99, #reads = 1080844 mem = 536878376 peak = 1271685120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286022, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.60, 126.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 4, 179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (51.75, 94.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 704)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675086 sequences (100000151 bp) in 2.421 CPU sec, 2.973 real sec...
[M::process] read 675124 sequences (100000053 bp) in 1.993 CPU sec, 2.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286052, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.81, 125.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675252 reads in 243.137 CPU sec, 7.915 real sec
[M::mem_process_seqs] Processed 675048 reads in 137.100 CPU sec, 4.444 real sec
sorting block 100, #reads = 1080728 mem = 536878705 peak = 1271685120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286039, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.29, 124.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 183, 606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1752)
[M::mem_pestat] mean and std.dev: (287.90, 266.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2325)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675082 sequences (100000127 bp) in 2.259 CPU sec, 2.997 real sec...
[M::process] read 674896 sequences (100000027 bp) in 2.035 CPU sec, 2.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285984, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.76, 127.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (293, 637, 863)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2003)
[M::mem_pestat] mean and std.dev: (627.80, 555.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2849)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675086 reads in 240.658 CPU sec, 7.832 real sec
[M::mem_process_seqs] Processed 675124 reads in 136.537 CPU sec, 4.395 real sec
sorting block 101, #reads = 1080844 mem = 536878800 peak = 1271685120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285834, 13, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (254.00, 127.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 42, 59)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 159)
[M::mem_pestat] mean and std.dev: (29.00, 23.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 209)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674998 sequences (100000090 bp) in 2.272 CPU sec, 3.146 real sec...
[M::process] read 675058 sequences (100000004 bp) in 2.009 CPU sec, 2.023 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286427, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.05, 127.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675082 reads in 238.099 CPU sec, 7.743 real sec
[M::mem_process_seqs] Processed 674896 reads in 134.960 CPU sec, 4.312 real sec
sorting block 102, #reads = 1080883 mem = 536880180 peak = 1271685120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285908, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.91, 127.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 73, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1716)
[M::mem_pestat] mean and std.dev: (246.92, 268.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2283)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674908 sequences (100000277 bp) in 2.340 CPU sec, 3.004 real sec...
sorting block 103, #reads = 1080781 mem = 536878234 peak = 1271685120
[M::process] read 674918 sequences (100000053 bp) in 1.875 CPU sec, 2.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286573, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.42, 129.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674998 reads in 240.632 CPU sec, 7.871 real sec
[M::mem_process_seqs] Processed 675058 reads in 135.901 CPU sec, 4.418 real sec
sorting block 104, #reads = 1080743 mem = 536877953 peak = 1271685120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285528, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.14, 130.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 210, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097)
[M::mem_pestat] mean and std.dev: (203.58, 157.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1452)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674908 sequences (100000128 bp) in 2.378 CPU sec, 2.892 real sec...
[M::process] read 674940 sequences (100000092 bp) in 1.986 CPU sec, 2.129 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285614, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.69, 129.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674908 reads in 241.217 CPU sec, 7.848 real sec
[M::mem_process_seqs] Processed 674918 reads in 135.902 CPU sec, 4.385 real sec
sorting block 105, #reads = 1080760 mem = 536878641 peak = 1271685120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285887, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.51, 130.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674928 sequences (100000113 bp) in 2.330 CPU sec, 2.822 real sec...
[M::process] read 674908 sequences (100000207 bp) in 1.940 CPU sec, 1.960 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285912, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.55, 131.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674908 reads in 241.967 CPU sec, 7.871 real sec
[M::mem_process_seqs] Processed 674940 reads in 136.120 CPU sec, 4.369 real sec
sorting block 106, #reads = 1080218 mem = 536880080 peak = 1271685120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285781, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.21, 131.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674838 sequences (100000085 bp) in 2.330 CPU sec, 3.056 real sec...
[M::process] read 674892 sequences (100000188 bp) in 1.995 CPU sec, 2.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285697, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.37, 130.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 23, 23)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 23)
[M::mem_pestat] mean and std.dev: (23.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (23, 23)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674928 reads in 244.337 CPU sec, 7.952 real sec
[M::mem_process_seqs] Processed 674908 reads in 137.909 CPU sec, 4.399 real sec
sorting block 107, #reads = 1080505 mem = 536880550 peak = 1271685120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285667, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.28, 131.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 53, 1521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4511)
[M::mem_pestat] mean and std.dev: (594.64, 705.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6006)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 108, #reads = 1080123 mem = 536880904 peak = 1271685120
[M::process] read 674780 sequences (100000129 bp) in 2.402 CPU sec, 3.006 real sec...
[M::process] read 674658 sequences (100000187 bp) in 1.936 CPU sec, 2.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285746, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.68, 132.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674838 reads in 242.342 CPU sec, 7.939 real sec
[M::mem_process_seqs] Processed 674892 reads in 136.783 CPU sec, 4.447 real sec
sorting block 109, #reads = 1080848 mem = 536881234 peak = 1271685120
[M::process] read 674702 sequences (100000189 bp) in 2.567 CPU sec, 2.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285615, 18, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.17, 132.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (306, 8318, 8318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24342)
[M::mem_pestat] mean and std.dev: (5223.28, 3892.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 32354)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674620 sequences (100000074 bp) in 1.932 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285538, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.74, 132.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674780 reads in 242.007 CPU sec, 7.855 real sec
[M::mem_process_seqs] Processed 674658 reads in 137.212 CPU sec, 4.420 real sec
sorting block 110, #reads = 1080063 mem = 536880407 peak = 1271685120
[M::process] read 674692 sequences (100000278 bp) in 2.475 CPU sec, 2.778 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285509, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.82, 132.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 176, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (221.00, 114.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674474 sequences (100000289 bp) in 2.044 CPU sec, 2.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285950, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.27, 133.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674702 reads in 241.297 CPU sec, 7.838 real sec
[M::mem_process_seqs] Processed 674620 reads in 135.818 CPU sec, 4.371 real sec
sorting block 111, #reads = 1080425 mem = 536879183 peak = 1271685120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285780, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.37, 132.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674586 sequences (100000019 bp) in 2.328 CPU sec, 2.982 real sec...
[M::process] read 674418 sequences (100000134 bp) in 1.930 CPU sec, 1.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286212, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.88, 134.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 273, 1109)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3181)
[M::mem_pestat] mean and std.dev: (419.00, 448.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4217)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674692 reads in 238.044 CPU sec, 7.730 real sec
[M::mem_process_seqs] Processed 674474 reads in 134.480 CPU sec, 4.292 real sec
sorting block 112, #reads = 1080188 mem = 536881740 peak = 1278517248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285833, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 113, #reads = 1080334 mem = 536879677 peak = 1278726144
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.95, 134.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674458 sequences (100000025 bp) in 2.375 CPU sec, 3.112 real sec...
[M::process] read 674658 sequences (100000256 bp) in 2.051 CPU sec, 2.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285496, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.81, 135.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674586 reads in 241.879 CPU sec, 7.929 real sec
[M::mem_process_seqs] Processed 674418 reads in 136.114 CPU sec, 4.425 real sec
sorting block 114, #reads = 1079948 mem = 536879701 peak = 1278726144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285927, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.18, 134.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 18, 1135)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3379)
[M::mem_pestat] mean and std.dev: (295.38, 476.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4501)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674494 sequences (100000096 bp) in 2.351 CPU sec, 3.097 real sec...
[M::process] read 674344 sequences (100000211 bp) in 2.030 CPU sec, 2.036 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285972, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.83, 135.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1558, 1558, 8627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22765)
[M::mem_pestat] mean and std.dev: (4520.71, 3911.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 29834)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674458 reads in 234.508 CPU sec, 7.633 real sec
[M::mem_process_seqs] Processed 674658 reads in 133.126 CPU sec, 4.300 real sec
sorting block 115, #reads = 1080056 mem = 536877425 peak = 1278726144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285725, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.92, 135.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674522 sequences (100000083 bp) in 2.364 CPU sec, 3.007 real sec...
[M::process] read 674446 sequences (100000123 bp) in 1.922 CPU sec, 1.960 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285255, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.23, 136.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674494 reads in 234.215 CPU sec, 7.625 real sec
[M::mem_process_seqs] Processed 674344 reads in 132.961 CPU sec, 4.257 real sec
sorting block 116, #reads = 1080517 mem = 536879472 peak = 1278726144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285923, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.60, 136.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 392, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112)
[M::mem_pestat] mean and std.dev: (211.44, 204.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1472)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675100 sequences (100000187 bp) in 2.393 CPU sec, 3.057 real sec...
[M::process] read 675564 sequences (100000227 bp) in 2.001 CPU sec, 2.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285958, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.24, 137.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (370, 577, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1456)
[M::mem_pestat] mean and std.dev: (461.00, 244.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1818)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674522 reads in 235.869 CPU sec, 7.682 real sec
sorting block 117, #reads = 1079991 mem = 536878686 peak = 1279672320
[M::mem_process_seqs] Processed 674446 reads in 133.525 CPU sec, 4.291 real sec
sorting block 118, #reads = 1080528 mem = 536879662 peak = 1279672320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286194, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.83, 132.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675326 sequences (100000253 bp) in 2.435 CPU sec, 2.959 real sec...
[M::process] read 675166 sequences (100000005 bp) in 1.976 CPU sec, 2.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285210, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (249.65, 126.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675100 reads in 248.306 CPU sec, 8.138 real sec
[M::mem_process_seqs] Processed 675564 reads in 145.984 CPU sec, 4.737 real sec
sorting block 119, #reads = 1080083 mem = 536881384 peak = 1279672320
[M::process] read 675080 sequences (100000126 bp) in 2.461 CPU sec, 2.897 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284573, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (252.76, 129.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675024 sequences (100000087 bp) in 2.052 CPU sec, 2.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285047, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.23, 131.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 45, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593)
[M::mem_pestat] mean and std.dev: (69.29, 77.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284404, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.62, 128.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675326 reads in 384.171 CPU sec, 12.473 real sec
[M::mem_process_seqs] Processed 675166 reads in 269.231 CPU sec, 8.676 real sec
[M::mem_process_seqs] Processed 675080 reads in 280.509 CPU sec, 9.059 real sec
sorting block 120, #reads = 1080076 mem = 536879429 peak = 1279672320
[M::process] read 675050 sequences (100000191 bp) in 2.604 CPU sec, 2.743 real sec...
sorting block 121, #reads = 1079888 mem = 536879423 peak = 1279672320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284939, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.29, 130.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675068 sequences (100000000 bp) in 2.702 CPU sec, 2.951 real sec...
[M::process] read 675148 sequences (100000106 bp) in 1.899 CPU sec, 1.920 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284951, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.71, 129.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 32, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1996)
[M::mem_pestat] mean and std.dev: (257.27, 312.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2657)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675024 reads in 265.892 CPU sec, 8.679 real sec
[M::mem_process_seqs] Processed 675050 reads in 149.092 CPU sec, 4.846 real sec
sorting block 122, #reads = 1080618 mem = 536879650 peak = 1279672320
[M::process] read 675096 sequences (100000197 bp) in 2.749 CPU sec, 2.869 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284609, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.82, 131.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 388, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114)
[M::mem_pestat] mean and std.dev: (208.56, 170.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1477)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675198 sequences (100000000 bp) in 1.964 CPU sec, 1.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285275, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.37, 132.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 8, 84)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236)
[M::mem_pestat] mean and std.dev: (24.09, 29.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 312)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675068 reads in 261.703 CPU sec, 8.491 real sec
[M::mem_process_seqs] Processed 675148 reads in 146.331 CPU sec, 4.684 real sec
sorting block 123, #reads = 1079674 mem = 536878763 peak = 1279672320
[M::process] read 675824 sequences (100000265 bp) in 2.653 CPU sec, 3.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284772, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (254.90, 130.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 128, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (217.81, 150.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1329)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675630 sequences (100000003 bp) in 1.926 CPU sec, 1.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284992, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.45, 130.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675096 reads in 263.417 CPU sec, 8.544 real sec
[M::mem_process_seqs] Processed 675198 reads in 146.433 CPU sec, 4.683 real sec
sorting block 124, #reads = 1080503 mem = 536878040 peak = 1279672320
sorting block 125, #reads = 1080336 mem = 536879127 peak = 1279672320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285735, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.05, 122.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675366 sequences (100000269 bp) in 2.693 CPU sec, 3.320 real sec...
[M::process] read 675466 sequences (100000110 bp) in 2.046 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285887, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (247.54, 123.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675824 reads in 256.889 CPU sec, 8.413 real sec
[M::mem_process_seqs] Processed 675630 reads in 145.735 CPU sec, 4.725 real sec
sorting block 126, #reads = 1080872 mem = 536878371 peak = 1279672320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284887, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.48, 128.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675386 sequences (100000290 bp) in 2.658 CPU sec, 3.418 real sec...
[M::process] read 675314 sequences (100000192 bp) in 1.987 CPU sec, 1.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285423, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.18, 127.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675366 reads in 259.787 CPU sec, 8.443 real sec
[M::mem_process_seqs] Processed 675466 reads in 145.493 CPU sec, 4.684 real sec
sorting block 127, #reads = 1080376 mem = 536880373 peak = 1279672320
[M::process] read 675542 sequences (100000030 bp) in 2.660 CPU sec, 3.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284822, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.52, 127.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 9, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (92.29, 141.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675280 sequences (100000145 bp) in 1.914 CPU sec, 1.972 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285009, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.75, 126.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675386 reads in 261.233 CPU sec, 8.491 real sec
[M::mem_process_seqs] Processed 675314 reads in 146.484 CPU sec, 4.692 real sec
sorting block 128, #reads = 1080740 mem = 536880890 peak = 1279672320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285141, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.11, 127.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 45, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152)
[M::mem_pestat] mean and std.dev: (166.50, 246.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1532)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675200 sequences (100000210 bp) in 2.598 CPU sec, 3.416 real sec...
[M::process] read 675366 sequences (100000068 bp) in 1.914 CPU sec, 1.920 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284885, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.23, 127.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 209, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (209, 209)
[M::mem_pestat] mean and std.dev: (209.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (209, 209)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675542 reads in 265.254 CPU sec, 8.596 real sec
sorting block 129, #reads = 1080478 mem = 536880230 peak = 1289338880
[M::mem_process_seqs] Processed 675280 reads in 149.986 CPU sec, 4.802 real sec
sorting block 130, #reads = 1080589 mem = 536879136 peak = 1289338880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284558, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.77, 126.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675444 sequences (100000187 bp) in 2.720 CPU sec, 3.373 real sec...
[M::process] read 675304 sequences (100000184 bp) in 1.993 CPU sec, 2.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285064, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.90, 124.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675200 reads in 265.837 CPU sec, 8.700 real sec
[M::mem_process_seqs] Processed 675366 reads in 147.013 CPU sec, 4.779 real sec
sorting block 131, #reads = 1080023 mem = 536882474 peak = 1289338880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285328, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.28, 124.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675172 sequences (100000042 bp) in 2.628 CPU sec, 3.403 real sec...
[M::process] read 675254 sequences (100000279 bp) in 2.047 CPU sec, 2.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285038, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.96, 126.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675444 reads in 268.407 CPU sec, 8.706 real sec
[M::mem_process_seqs] Processed 675304 reads in 150.568 CPU sec, 4.842 real sec
sorting block 132, #reads = 1080645 mem = 536879039 peak = 1289338880
[M::process] read 675184 sequences (100000173 bp) in 2.745 CPU sec, 3.281 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284853, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.75, 126.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675294 sequences (100000015 bp) in 1.917 CPU sec, 1.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285169, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.38, 127.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675172 reads in 268.367 CPU sec, 8.720 real sec
[M::mem_process_seqs] Processed 675254 reads in 148.666 CPU sec, 4.757 real sec
sorting block 133, #reads = 1080687 mem = 536878126 peak = 1289338880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284844, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.56, 127.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 409, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177)
[M::mem_pestat] mean and std.dev: (238.50, 192.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1561)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675308 sequences (100000252 bp) in 2.617 CPU sec, 3.342 real sec...
[M::process] read 675216 sequences (100000297 bp) in 1.903 CPU sec, 1.912 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285034, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.86, 127.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675184 reads in 265.316 CPU sec, 8.606 real sec
sorting block 134, #reads = 1081113 mem = 536877944 peak = 1289338880
[M::mem_process_seqs] Processed 675294 reads in 147.880 CPU sec, 4.731 real sec
sorting block 135, #reads = 1080403 mem = 536881531 peak = 1289338880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284928, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.95, 128.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675138 sequences (100000216 bp) in 2.779 CPU sec, 3.260 real sec...
[M::process] read 675022 sequences (100000188 bp) in 2.098 CPU sec, 2.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285451, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.26, 127.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 14, 26)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 60)
[M::mem_pestat] mean and std.dev: (16.71, 11.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 77)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675308 reads in 264.407 CPU sec, 8.646 real sec
[M::mem_process_seqs] Processed 675216 reads in 145.966 CPU sec, 4.725 real sec
sorting block 136, #reads = 1080563 mem = 536879659 peak = 1289338880
[M::process] read 675162 sequences (100000026 bp) in 2.745 CPU sec, 3.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285125, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.00, 128.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675172 sequences (100000279 bp) in 2.064 CPU sec, 2.281 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285337, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.66, 129.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675138 reads in 265.549 CPU sec, 8.627 real sec
[M::mem_process_seqs] Processed 675022 reads in 147.179 CPU sec, 4.733 real sec
sorting block 137, #reads = 1080789 mem = 536878352 peak = 1289338880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285136, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.03, 128.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 210, 1528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4178)
[M::mem_pestat] mean and std.dev: (353.11, 426.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5503)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674694 sequences (100000113 bp) in 2.600 CPU sec, 3.511 real sec...
[M::process] read 674222 sequences (100000218 bp) in 1.973 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285055, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.55, 130.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675162 reads in 267.228 CPU sec, 8.681 real sec
[M::mem_process_seqs] Processed 675172 reads in 149.845 CPU sec, 4.792 real sec
sorting block 138, #reads = 1081151 mem = 536880883 peak = 1289338880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283830, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.26, 136.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674270 sequences (100000198 bp) in 2.739 CPU sec, 3.346 real sec...
sorting block 139, #reads = 1080807 mem = 536880547 peak = 1289338880
[M::process] read 674794 sequences (100000144 bp) in 1.924 CPU sec, 2.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283871, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.33, 144.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 1108, 1108)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3316)
[M::mem_pestat] mean and std.dev: (558.80, 549.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4420)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674694 reads in 274.738 CPU sec, 8.981 real sec
[M::mem_process_seqs] Processed 674222 reads in 154.571 CPU sec, 5.010 real sec
sorting block 140, #reads = 1079615 mem = 536880636 peak = 1289338880
[M::process] read 675006 sequences (100000274 bp) in 2.790 CPU sec, 3.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283784, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (273.83, 146.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 15, 19)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 41)
[M::mem_pestat] mean and std.dev: (12.85, 5.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 52)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674990 sequences (100000206 bp) in 2.080 CPU sec, 2.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284871, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (260.76, 135.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674270 reads in 270.411 CPU sec, 8.790 real sec
[M::mem_process_seqs] Processed 674794 reads in 150.066 CPU sec, 4.852 real sec
sorting block 141, #reads = 1079860 mem = 536877421 peak = 1289338880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285331, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.30, 130.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 177, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418)
[M::mem_pestat] mean and std.dev: (286.50, 365.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1890)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674682 sequences (100000023 bp) in 2.660 CPU sec, 3.413 real sec...
[M::process] read 674302 sequences (100000091 bp) in 2.086 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285022, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.99, 131.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675006 reads in 263.104 CPU sec, 8.554 real sec
[M::mem_process_seqs] Processed 674990 reads in 149.266 CPU sec, 4.790 real sec
sorting block 142, #reads = 1080254 mem = 536880078 peak = 1289338880
[M::process] read 674258 sequences (100000176 bp) in 2.765 CPU sec, 3.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284637, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.67, 136.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674734 sequences (100000120 bp) in 1.940 CPU sec, 1.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284026, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (273.28, 146.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 414, 768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2108)
[M::mem_pestat] mean and std.dev: (389.33, 300.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2778)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674682 reads in 272.945 CPU sec, 8.863 real sec
[M::mem_process_seqs] Processed 674302 reads in 153.168 CPU sec, 4.900 real sec
sorting block 143, #reads = 1080601 mem = 536876474 peak = 1289338880
[M::process] read 675008 sequences (100000091 bp) in 2.796 CPU sec, 3.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284333, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (272.86, 146.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 20, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294)
[M::mem_pestat] mean and std.dev: (46.94, 75.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 389)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 144, #reads = 1079795 mem = 536876812 peak = 1296916480
[M::process] read 674866 sequences (100000079 bp) in 1.885 CPU sec, 2.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285013, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.08, 137.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674258 reads in 269.928 CPU sec, 8.817 real sec
[M::mem_process_seqs] Processed 674734 reads in 147.272 CPU sec, 4.773 real sec
sorting block 145, #reads = 1078297 mem = 536877082 peak = 1296916480
[M::process] read 674886 sequences (100000182 bp) in 2.825 CPU sec, 3.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285236, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (259.08, 134.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 13, 152)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440)
[M::mem_pestat] mean and std.dev: (27.89, 45.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675334 sequences (100000268 bp) in 2.105 CPU sec, 2.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284736, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (259.93, 136.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675008 reads in 261.473 CPU sec, 8.499 real sec
[M::mem_process_seqs] Processed 674866 reads in 146.590 CPU sec, 4.738 real sec
sorting block 146, #reads = 1080103 mem = 536880341 peak = 1296916480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284944, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.25, 136.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 44, 1425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4247)
[M::mem_pestat] mean and std.dev: (453.67, 627.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5658)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675688 sequences (100000093 bp) in 2.778 CPU sec, 3.371 real sec...
[M::process] read 675254 sequences (100000226 bp) in 1.995 CPU sec, 2.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285622, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (252.66, 130.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 8, 88)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258)
[M::mem_pestat] mean and std.dev: (45.20, 50.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 343)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674886 reads in 256.155 CPU sec, 8.333 real sec
[M::mem_process_seqs] Processed 675334 reads in 142.372 CPU sec, 4.570 real sec
sorting block 147, #reads = 1079628 mem = 536878996 peak = 1296916480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285749, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.65, 123.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675166 sequences (100000078 bp) in 2.822 CPU sec, 3.543 real sec...
[M::process] read 675154 sequences (100000238 bp) in 2.044 CPU sec, 2.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285011, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.71, 128.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675688 reads in 253.796 CPU sec, 8.235 real sec
[M::mem_process_seqs] Processed 675254 reads in 144.039 CPU sec, 4.596 real sec
sorting block 148, #reads = 1080198 mem = 536879192 peak = 1297465344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285173, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (254.02, 127.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 149, #reads = 1080134 mem = 536879145 peak = 1297469440
[M::process] read 675160 sequences (100000223 bp) in 2.855 CPU sec, 3.430 real sec...
[M::process] read 675232 sequences (100000180 bp) in 1.935 CPU sec, 2.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285546, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.68, 128.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (55, 58, 1121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3253)
[M::mem_pestat] mean and std.dev: (372.00, 464.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4319)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675166 reads in 254.405 CPU sec, 8.330 real sec
[M::mem_process_seqs] Processed 675154 reads in 143.020 CPU sec, 4.648 real sec
sorting block 150, #reads = 1079867 mem = 536879748 peak = 1297469440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285564, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.90, 126.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 42, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 197)
[M::mem_pestat] mean and std.dev: (46.58, 39.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 259)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675084 sequences (100000296 bp) in 2.799 CPU sec, 3.077 real sec...
[M::process] read 675108 sequences (100000201 bp) in 1.965 CPU sec, 2.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285227, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.24, 127.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675160 reads in 250.259 CPU sec, 8.133 real sec
[M::mem_process_seqs] Processed 675232 reads in 143.344 CPU sec, 4.649 real sec
sorting block 151, #reads = 1080173 mem = 536879235 peak = 1297469440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285750, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.90, 129.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675094 sequences (100000275 bp) in 2.719 CPU sec, 3.378 real sec...
[M::process] read 675076 sequences (100000047 bp) in 1.989 CPU sec, 2.041 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285434, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.90, 127.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675084 reads in 252.084 CPU sec, 8.188 real sec
[M::mem_process_seqs] Processed 675108 reads in 141.351 CPU sec, 4.517 real sec
sorting block 152, #reads = 1080234 mem = 536878875 peak = 1297469440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285679, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.55, 125.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675174 sequences (100000202 bp) in 2.719 CPU sec, 3.333 real sec...
[M::process] read 675368 sequences (100000218 bp) in 2.000 CPU sec, 2.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285765, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.31, 125.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675094 reads in 244.693 CPU sec, 7.949 real sec
[M::mem_process_seqs] Processed 675076 reads in 139.173 CPU sec, 4.447 real sec
sorting block 153, #reads = 1080248 mem = 536880106 peak = 1297469440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285904, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.79, 123.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 154, #reads = 1080177 mem = 536877030 peak = 1297469440
[M::process] read 675464 sequences (100000128 bp) in 2.721 CPU sec, 3.509 real sec...
[M::process] read 675498 sequences (100000091 bp) in 2.074 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285865, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.61, 122.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675174 reads in 246.842 CPU sec, 8.095 real sec
[M::mem_process_seqs] Processed 675368 reads in 140.535 CPU sec, 4.568 real sec
sorting block 155, #reads = 1080302 mem = 536879257 peak = 1297469440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285825, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.34, 122.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 348, 668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1600)
[M::mem_pestat] mean and std.dev: (476.38, 378.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2066)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675418 sequences (100000257 bp) in 2.849 CPU sec, 3.380 real sec...
[M::process] read 675494 sequences (100000007 bp) in 2.112 CPU sec, 2.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285673, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.45, 124.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 33, 33)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 71)
[M::mem_pestat] mean and std.dev: (24.22, 14.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 90)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675464 reads in 253.633 CPU sec, 8.253 real sec
[M::mem_process_seqs] Processed 675498 reads in 145.009 CPU sec, 4.690 real sec
sorting block 156, #reads = 1080591 mem = 536881931 peak = 1297469440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285558, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.09, 124.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 217, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146)
[M::mem_pestat] mean and std.dev: (209.26, 147.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1515)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675366 sequences (100000150 bp) in 2.658 CPU sec, 3.655 real sec...
[M::process] read 675256 sequences (100000029 bp) in 2.094 CPU sec, 2.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285182, 22, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.61, 123.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 132, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (221.64, 209.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1385)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675418 reads in 258.008 CPU sec, 8.358 real sec
[M::mem_process_seqs] Processed 675494 reads in 147.268 CPU sec, 4.694 real sec
sorting block 157, #reads = 1080730 mem = 536881836 peak = 1297469440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285224, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.84, 124.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675360 sequences (100000024 bp) in 2.829 CPU sec, 3.378 real sec...
[M::process] read 675372 sequences (100000048 bp) in 1.976 CPU sec, 1.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285216, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.62, 126.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675366 reads in 254.867 CPU sec, 8.267 real sec
sorting block 158, #reads = 1080738 mem = 536880908 peak = 1297469440
[M::mem_process_seqs] Processed 675256 reads in 144.264 CPU sec, 4.616 real sec
sorting block 159, #reads = 1080122 mem = 536880751 peak = 1298202624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285580, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.01, 125.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 66, 67)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 185)
[M::mem_pestat] mean and std.dev: (37.71, 29.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 244)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675310 sequences (100000220 bp) in 2.854 CPU sec, 3.550 real sec...
[M::process] read 675318 sequences (100000174 bp) in 2.054 CPU sec, 2.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285625, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.63, 125.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (37, 76, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1672)
[M::mem_pestat] mean and std.dev: (121.38, 175.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2217)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675360 reads in 260.674 CPU sec, 8.530 real sec
[M::mem_process_seqs] Processed 675372 reads in 146.161 CPU sec, 4.754 real sec
sorting block 160, #reads = 1080810 mem = 536879734 peak = 1298202624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285120, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.42, 125.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 16, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215)
[M::mem_pestat] mean and std.dev: (34.82, 36.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 286)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675286 sequences (100000171 bp) in 2.903 CPU sec, 3.552 real sec...
[M::process] read 675286 sequences (100000019 bp) in 2.011 CPU sec, 2.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285218, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.81, 125.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675310 reads in 260.846 CPU sec, 8.490 real sec
[M::mem_process_seqs] Processed 675318 reads in 145.194 CPU sec, 4.687 real sec
sorting block 161, #reads = 1080915 mem = 536879959 peak = 1298202624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285060, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.42, 126.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675240 sequences (100000250 bp) in 2.774 CPU sec, 3.396 real sec...
[M::process] read 675394 sequences (100000227 bp) in 1.952 CPU sec, 2.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285211, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.00, 127.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675286 reads in 262.318 CPU sec, 8.526 real sec
[M::mem_process_seqs] Processed 675286 reads in 147.222 CPU sec, 4.719 real sec
sorting block 162, #reads = 1080914 mem = 536881121 peak = 1298202624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285315, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.71, 127.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675182 sequences (100000293 bp) in 2.797 CPU sec, 3.605 real sec...
[M::process] read 675280 sequences (100000100 bp) in 2.114 CPU sec, 2.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285656, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.83, 126.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675240 reads in 261.252 CPU sec, 8.445 real sec
sorting block 163, #reads = 1080236 mem = 536877393 peak = 1298231296
[M::mem_process_seqs] Processed 675394 reads in 147.577 CPU sec, 4.696 real sec
sorting block 164, #reads = 1080669 mem = 536878673 peak = 1298231296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285306, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.71, 127.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675188 sequences (100000226 bp) in 2.953 CPU sec, 3.296 real sec...
[M::process] read 675078 sequences (100000053 bp) in 2.035 CPU sec, 2.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285563, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.45, 126.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675182 reads in 257.350 CPU sec, 8.429 real sec
[M::mem_process_seqs] Processed 675280 reads in 144.221 CPU sec, 4.690 real sec
sorting block 165, #reads = 1080483 mem = 536878654 peak = 1298231296
[M::process] read 675140 sequences (100000239 bp) in 2.935 CPU sec, 3.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285216, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.54, 128.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 14, 42)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 120)
[M::mem_pestat] mean and std.dev: (16.50, 15.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 159)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675170 sequences (100000100 bp) in 1.995 CPU sec, 2.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284834, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.35, 128.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 19, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (82.69, 129.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675188 reads in 263.257 CPU sec, 8.543 real sec
[M::mem_process_seqs] Processed 675078 reads in 148.899 CPU sec, 4.788 real sec
sorting block 166, #reads = 1080591 mem = 536877452 peak = 1298231296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285008, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.64, 130.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 514, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278)
[M::mem_pestat] mean and std.dev: (311.92, 228.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1649)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675082 sequences (100000086 bp) in 2.596 CPU sec, 3.324 real sec...
[M::process] read 674684 sequences (100000181 bp) in 1.954 CPU sec, 1.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285189, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.27, 130.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675140 reads in 262.674 CPU sec, 8.531 real sec
[M::mem_process_seqs] Processed 675170 reads in 147.099 CPU sec, 4.701 real sec
sorting block 167, #reads = 1080270 mem = 536879779 peak = 1298231296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284553, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.35, 130.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674274 sequences (100000190 bp) in 2.749 CPU sec, 3.195 real sec...
sorting block 168, #reads = 1080847 mem = 536880240 peak = 1298489344
[M::process] read 674706 sequences (100000202 bp) in 2.017 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283582, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (269.11, 144.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675082 reads in 268.548 CPU sec, 8.790 real sec
[M::mem_process_seqs] Processed 674684 reads in 151.036 CPU sec, 4.913 real sec
sorting block 169, #reads = 1079834 mem = 536879012 peak = 1298489344
[M::process] read 675178 sequences (100000108 bp) in 2.737 CPU sec, 3.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283665, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (276.77, 150.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675022 sequences (100000267 bp) in 2.126 CPU sec, 2.281 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284870, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (264.01, 139.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285259, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (253.79, 131.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 87, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273)
[M::mem_pestat] mean and std.dev: (205.31, 218.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1687)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674274 reads in 399.021 CPU sec, 12.931 real sec
[M::mem_process_seqs] Processed 674706 reads in 273.635 CPU sec, 8.829 real sec
[M::mem_process_seqs] Processed 675178 reads in 284.886 CPU sec, 9.212 real sec
sorting block 170, #reads = 1079697 mem = 536878455 peak = 1298489344
[M::process] read 674912 sequences (100000010 bp) in 2.948 CPU sec, 3.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285094, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.90, 132.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 171, #reads = 1080195 mem = 536878751 peak = 1298489344
[M::process] read 674870 sequences (100000009 bp) in 2.696 CPU sec, 3.152 real sec...
[M::process] read 674458 sequences (100000032 bp) in 2.062 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284441, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.58, 133.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 196, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184)
[M::mem_pestat] mean and std.dev: (174.80, 146.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1568)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675022 reads in 270.634 CPU sec, 8.843 real sec
[M::mem_process_seqs] Processed 674912 reads in 153.497 CPU sec, 4.987 real sec
sorting block 172, #reads = 1080390 mem = 536880405 peak = 1298489344
[M::process] read 674870 sequences (100000032 bp) in 2.640 CPU sec, 2.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284736, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (265.12, 142.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (278, 704, 1811)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4877)
[M::mem_pestat] mean and std.dev: (987.45, 772.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6410)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674958 sequences (100000015 bp) in 2.167 CPU sec, 2.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284149, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (270.09, 146.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674870 reads in 268.415 CPU sec, 8.716 real sec
[M::mem_process_seqs] Processed 674458 reads in 151.294 CPU sec, 4.877 real sec
sorting block 173, #reads = 1080051 mem = 536878711 peak = 1298489344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284378, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (261.48, 139.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 69, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 197)
[M::mem_pestat] mean and std.dev: (38.91, 29.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 260)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675006 sequences (100000105 bp) in 2.512 CPU sec, 3.366 real sec...
[M::process] read 675180 sequences (100000216 bp) in 2.148 CPU sec, 2.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285402, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.31, 135.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674870 reads in 262.714 CPU sec, 8.528 real sec
[M::mem_process_seqs] Processed 674958 reads in 145.057 CPU sec, 4.612 real sec
sorting block 174, #reads = 1080079 mem = 536877356 peak = 1298489344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285009, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.00, 135.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675352 sequences (100000254 bp) in 2.631 CPU sec, 3.218 real sec...
[M::process] read 675442 sequences (100000244 bp) in 2.131 CPU sec, 2.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285162, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.67, 135.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675006 reads in 258.501 CPU sec, 8.391 real sec
sorting block 175, #reads = 1080268 mem = 536878012 peak = 1298489344
[M::mem_process_seqs] Processed 675180 reads in 143.735 CPU sec, 4.597 real sec
sorting block 176, #reads = 1079887 mem = 536877119 peak = 1298677760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285873, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (251.54, 129.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675046 sequences (100000276 bp) in 2.695 CPU sec, 3.100 real sec...
[M::process] read 675086 sequences (100000009 bp) in 1.970 CPU sec, 2.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286092, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (248.87, 124.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675352 reads in 247.467 CPU sec, 8.095 real sec
[M::mem_process_seqs] Processed 675442 reads in 143.268 CPU sec, 4.647 real sec
sorting block 177, #reads = 1080236 mem = 536881784 peak = 1298677760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285642, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.21, 128.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 389, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1628)
[M::mem_pestat] mean and std.dev: (201.00, 225.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2166)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675144 sequences (100000143 bp) in 2.602 CPU sec, 3.186 real sec...
[M::process] read 674914 sequences (100000282 bp) in 1.991 CPU sec, 2.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285657, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.51, 129.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 61, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 131)
[M::mem_pestat] mean and std.dev: (45.60, 24.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 166)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675046 reads in 250.627 CPU sec, 8.142 real sec
[M::mem_process_seqs] Processed 675086 reads in 140.643 CPU sec, 4.527 real sec
sorting block 178, #reads = 1080909 mem = 536880614 peak = 1298677760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285575, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.85, 129.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674992 sequences (100000055 bp) in 2.622 CPU sec, 3.195 real sec...
[M::process] read 674952 sequences (100000089 bp) in 2.011 CPU sec, 2.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285151, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.09, 130.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 10, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (92.09, 134.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675144 reads in 250.713 CPU sec, 8.143 real sec
sorting block 179, #reads = 1080294 mem = 536881481 peak = 1298677760
[M::process] read 674886 sequences (100000183 bp) in 2.201 CPU sec, 2.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285564, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.75, 129.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674992 reads in 136.120 CPU sec, 4.425 real sec
sorting block 180, #reads = 1080229 mem = 536879734 peak = 1298677760
[M::mem_process_seqs] Processed 674914 reads in 331.608 CPU sec, 10.705 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285651, 24, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.50, 130.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 196, 9130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 27360)
[M::mem_pestat] mean and std.dev: (2423.88, 3879.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 36475)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674958 sequences (100000287 bp) in 2.403 CPU sec, 3.285 real sec...
[M::process] read 674980 sequences (100000112 bp) in 2.089 CPU sec, 2.102 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285299, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (258.08, 131.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1764, 1764, 1764)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1764, 1764)
[M::mem_pestat] mean and std.dev: (1764.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1764, 1764)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674952 reads in 266.425 CPU sec, 8.616 real sec
[M::mem_process_seqs] Processed 674886 reads in 145.591 CPU sec, 4.652 real sec
sorting block 181, #reads = 1079760 mem = 536878839 peak = 1299152896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285656, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.43, 130.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675054 sequences (100000068 bp) in 2.754 CPU sec, 3.434 real sec...
[M::process] read 675104 sequences (100000185 bp) in 1.960 CPU sec, 1.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285592, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.60, 129.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 18, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (47.08, 67.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 769)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674958 reads in 248.956 CPU sec, 8.068 real sec
sorting block 182, #reads = 1079885 mem = 536880840 peak = 1299152896
[M::mem_process_seqs] Processed 674980 reads in 140.092 CPU sec, 4.471 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285846, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.25, 129.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 364, 2395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7161)
[M::mem_pestat] mean and std.dev: (891.92, 1079.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9544)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 183, #reads = 1080516 mem = 536879500 peak = 1299152896
[M::process] read 675234 sequences (100000019 bp) in 2.609 CPU sec, 3.087 real sec...
[M::process] read 675452 sequences (100000035 bp) in 1.986 CPU sec, 2.003 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285069, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.94, 130.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675054 reads in 253.223 CPU sec, 8.284 real sec
[M::mem_process_seqs] Processed 675104 reads in 145.157 CPU sec, 4.711 real sec
sorting block 184, #reads = 1080230 mem = 536877926 peak = 1299152896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285397, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.98, 125.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675648 sequences (100000257 bp) in 2.532 CPU sec, 3.414 real sec...
[M::process] read 675340 sequences (100000017 bp) in 2.114 CPU sec, 2.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286103, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.03, 121.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675234 reads in 247.408 CPU sec, 8.028 real sec
[M::mem_process_seqs] Processed 675452 reads in 139.729 CPU sec, 4.501 real sec
sorting block 185, #reads = 1080446 mem = 536877943 peak = 1299152896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286383, 19, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.10, 121.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 58, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (117.71, 170.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675208 sequences (100000141 bp) in 2.479 CPU sec, 3.075 real sec...
[M::process] read 675180 sequences (100000050 bp) in 1.968 CPU sec, 1.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285486, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (246.29, 122.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675648 reads in 252.937 CPU sec, 8.221 real sec
[M::mem_process_seqs] Processed 675340 reads in 144.926 CPU sec, 4.628 real sec
sorting block 186, #reads = 1081374 mem = 536881062 peak = 1299152896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285854, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.29, 123.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 58, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499)
[M::mem_pestat] mean and std.dev: (176.41, 216.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1993)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675234 sequences (100000212 bp) in 2.540 CPU sec, 3.063 real sec...
[M::process] read 675270 sequences (100000209 bp) in 1.946 CPU sec, 1.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285289, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.91, 124.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675208 reads in 254.880 CPU sec, 8.267 real sec
sorting block 187, #reads = 1081006 mem = 536880495 peak = 1299828736
[M::mem_process_seqs] Processed 675180 reads in 145.702 CPU sec, 4.646 real sec
sorting block 188, #reads = 1080741 mem = 536877000 peak = 1299869696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284983, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.27, 125.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675326 sequences (100000167 bp) in 2.516 CPU sec, 3.335 real sec...
[M::process] read 675362 sequences (100000162 bp) in 2.103 CPU sec, 2.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285308, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.92, 127.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675234 reads in 257.584 CPU sec, 8.442 real sec
[M::mem_process_seqs] Processed 675270 reads in 144.867 CPU sec, 4.695 real sec
sorting block 189, #reads = 1080206 mem = 536879052 peak = 1299869696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285940, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.70, 129.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 51, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262)
[M::mem_pestat] mean and std.dev: (33.00, 35.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 348)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675338 sequences (100000045 bp) in 2.650 CPU sec, 3.255 real sec...
[M::process] read 675326 sequences (100000056 bp) in 2.027 CPU sec, 2.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285446, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.38, 126.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (264, 377, 1104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2784)
[M::mem_pestat] mean and std.dev: (476.07, 340.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3624)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675326 reads in 255.868 CPU sec, 8.326 real sec
[M::mem_process_seqs] Processed 675362 reads in 144.629 CPU sec, 4.655 real sec
sorting block 190, #reads = 1080760 mem = 536879580 peak = 1299869696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285213, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.90, 127.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 108, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1992)
[M::mem_pestat] mean and std.dev: (142.33, 202.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2651)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675268 sequences (100000281 bp) in 2.608 CPU sec, 3.524 real sec...
[M::process] read 675206 sequences (100000142 bp) in 2.014 CPU sec, 2.019 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285348, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.91, 128.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675338 reads in 259.670 CPU sec, 8.426 real sec
[M::mem_process_seqs] Processed 675326 reads in 146.190 CPU sec, 4.658 real sec
sorting block 191, #reads = 1080886 mem = 536880564 peak = 1299869696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285440, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.16, 129.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 73, 88)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174)
[M::mem_pestat] mean and std.dev: (61.36, 34.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 217)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675172 sequences (100000048 bp) in 2.759 CPU sec, 3.239 real sec...
[M::process] read 675106 sequences (100000089 bp) in 1.939 CPU sec, 1.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285297, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (251.98, 128.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675268 reads in 262.078 CPU sec, 8.508 real sec
sorting block 192, #reads = 1080507 mem = 536877048 peak = 1299869696
[M::mem_process_seqs] Processed 675206 reads in 147.475 CPU sec, 4.719 real sec
sorting block 193, #reads = 1080435 mem = 536879495 peak = 1299869696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285037, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.42, 129.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675232 sequences (100000002 bp) in 2.553 CPU sec, 3.194 real sec...
[M::process] read 675168 sequences (100000241 bp) in 2.104 CPU sec, 2.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285539, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.52, 129.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675172 reads in 259.969 CPU sec, 8.503 real sec
[M::mem_process_seqs] Processed 675106 reads in 145.766 CPU sec, 4.734 real sec
sorting block 194, #reads = 1080555 mem = 536878487 peak = 1299869696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285717, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 675112 sequences (100000132 bp) in 2.933 CPU sec, 3.137 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.76, 129.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 121, 770)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2284)
[M::mem_pestat] mean and std.dev: (145.64, 201.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3041)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675008 sequences (100000257 bp) in 2.039 CPU sec, 2.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285181, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.89, 130.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675232 reads in 259.558 CPU sec, 8.447 real sec
[M::mem_process_seqs] Processed 675168 reads in 146.216 CPU sec, 4.712 real sec
sorting block 195, #reads = 1080642 mem = 536882644 peak = 1299869696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285302, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.20, 130.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675096 sequences (100000168 bp) in 2.466 CPU sec, 3.536 real sec...
[M::process] read 675118 sequences (100000299 bp) in 2.096 CPU sec, 2.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285322, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.30, 131.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675112 reads in 257.694 CPU sec, 8.377 real sec
[M::mem_process_seqs] Processed 675008 reads in 145.496 CPU sec, 4.647 real sec
sorting block 196, #reads = 1080425 mem = 536881015 peak = 1299869696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285456, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.27, 131.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 197, #reads = 1079982 mem = 536879703 peak = 1299869696
[M::process] read 674964 sequences (100000031 bp) in 2.721 CPU sec, 3.385 real sec...
[M::process] read 674898 sequences (100000120 bp) in 2.058 CPU sec, 2.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284726, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.54, 132.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675096 reads in 264.231 CPU sec, 8.641 real sec
[M::mem_process_seqs] Processed 675118 reads in 147.916 CPU sec, 4.794 real sec
sorting block 198, #reads = 1080596 mem = 536879952 peak = 1299869696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285744, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.82, 134.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 17, 75)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199)
[M::mem_pestat] mean and std.dev: (29.93, 26.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 261)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674962 sequences (100000050 bp) in 2.576 CPU sec, 3.224 real sec...
[M::process] read 674974 sequences (100000071 bp) in 2.137 CPU sec, 2.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284967, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.37, 134.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674964 reads in 260.680 CPU sec, 8.470 real sec
[M::mem_process_seqs] Processed 674898 reads in 145.593 CPU sec, 4.714 real sec
sorting block 199, #reads = 1080848 mem = 536880960 peak = 1299869696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285690, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.41, 132.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674960 sequences (100000045 bp) in 2.829 CPU sec, 3.575 real sec...
[M::process] read 674930 sequences (100000295 bp) in 2.128 CPU sec, 2.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284884, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.68, 133.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 290, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (178.27, 141.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674962 reads in 257.879 CPU sec, 8.370 real sec
[M::mem_process_seqs] Processed 674974 reads in 144.717 CPU sec, 4.637 real sec
sorting block 200, #reads = 1080199 mem = 536879672 peak = 1299869696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285268, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.23, 135.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (54, 382, 9616)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28740)
[M::mem_pestat] mean and std.dev: (3100.90, 4275.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38302)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674988 sequences (100000155 bp) in 2.645 CPU sec, 3.229 real sec...
[M::process] read 675058 sequences (100000128 bp) in 2.015 CPU sec, 2.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285847, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.41, 133.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674960 reads in 255.961 CPU sec, 8.302 real sec
sorting block 201, #reads = 1080286 mem = 536880259 peak = 1299869696
[M::process] read 674910 sequences (100000206 bp) in 2.445 CPU sec, 2.479 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285016, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.95, 134.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 2155, 2155)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6421)
[M::mem_pestat] mean and std.dev: (1092.00, 1063.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8554)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674988 reads in 135.136 CPU sec, 4.414 real sec
sorting block 202, #reads = 1080649 mem = 536878837 peak = 1299869696
[M::mem_process_seqs] Processed 674930 reads in 328.653 CPU sec, 10.635 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284981, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.67, 135.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 218, 1804)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5266)
[M::mem_pestat] mean and std.dev: (811.07, 920.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6997)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675046 sequences (100000001 bp) in 2.635 CPU sec, 3.355 real sec...
[M::process] read 675034 sequences (100000072 bp) in 2.098 CPU sec, 2.111 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285385, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.43, 135.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 163, 872)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2566)
[M::mem_pestat] mean and std.dev: (311.46, 376.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3413)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675058 reads in 266.111 CPU sec, 8.641 real sec
[M::mem_process_seqs] Processed 674910 reads in 145.097 CPU sec, 4.683 real sec
sorting block 203, #reads = 1080516 mem = 536878311 peak = 1299869696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285616, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (258.85, 136.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675164 sequences (100000169 bp) in 2.737 CPU sec, 3.752 real sec...
[M::process] read 675730 sequences (100000196 bp) in 2.040 CPU sec, 2.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285604, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (257.70, 135.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (63, 88, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1281)
[M::mem_pestat] mean and std.dev: (119.10, 141.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1687)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675046 reads in 249.284 CPU sec, 8.069 real sec
sorting block 204, #reads = 1080206 mem = 536878689 peak = 1300226048
[M::mem_process_seqs] Processed 675034 reads in 140.705 CPU sec, 4.480 real sec
sorting block 205, #reads = 1080495 mem = 536877226 peak = 1300226048
[M::process] read 675458 sequences (100000235 bp) in 2.708 CPU sec, 3.143 real sec...
[M::process] read 675386 sequences (100000048 bp) in 2.091 CPU sec, 2.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286032, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (245.85, 124.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 66, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244)
[M::mem_pestat] mean and std.dev: (112.94, 149.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1656)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675730 reads in 133.152 CPU sec, 4.318 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285917, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (255.96, 134.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675336 sequences (100000095 bp) in 2.178 CPU sec, 2.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285984, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.24, 127.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 179, 179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473)
[M::mem_pestat] mean and std.dev: (145.50, 114.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675164 reads in 374.400 CPU sec, 12.154 real sec
[M::mem_process_seqs] Processed 675458 reads in 150.039 CPU sec, 4.817 real sec
sorting block 206, #reads = 1080785 mem = 536879399 peak = 1300226048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285885, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.41, 128.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 207, #reads = 1080361 mem = 536881307 peak = 1300226048
[M::process] read 675260 sequences (100000007 bp) in 2.939 CPU sec, 3.668 real sec...
[M::process] read 675238 sequences (100000120 bp) in 1.999 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285451, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.56, 130.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675386 reads in 249.332 CPU sec, 8.151 real sec
[M::mem_process_seqs] Processed 675336 reads in 141.467 CPU sec, 4.605 real sec
sorting block 208, #reads = 1080366 mem = 536881832 peak = 1300226048
[M::process] read 675140 sequences (100000154 bp) in 2.570 CPU sec, 2.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285099, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.32, 132.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 241, 704)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1980)
[M::mem_pestat] mean and std.dev: (363.83, 386.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2618)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675230 sequences (100000260 bp) in 1.949 CPU sec, 1.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285256, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.57, 133.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675260 reads in 247.177 CPU sec, 8.046 real sec
[M::mem_process_seqs] Processed 675238 reads in 140.135 CPU sec, 4.524 real sec
sorting block 209, #reads = 1079824 mem = 536880924 peak = 1300226048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285437, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.86, 133.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675068 sequences (100000249 bp) in 2.767 CPU sec, 3.301 real sec...
[M::process] read 674960 sequences (100000085 bp) in 1.927 CPU sec, 1.937 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285629, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (259.42, 134.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675140 reads in 248.308 CPU sec, 8.062 real sec
[M::mem_process_seqs] Processed 675230 reads in 142.377 CPU sec, 4.561 real sec
sorting block 210, #reads = 1080340 mem = 536880747 peak = 1300226048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285693, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.13, 132.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675006 sequences (100000231 bp) in 2.444 CPU sec, 3.088 real sec...
[M::process] read 674850 sequences (100000254 bp) in 1.923 CPU sec, 1.958 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285173, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.33, 133.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675068 reads in 250.338 CPU sec, 8.143 real sec
sorting block 211, #reads = 1080814 mem = 536878027 peak = 1300226048
[M::mem_process_seqs] Processed 674960 reads in 140.848 CPU sec, 4.495 real sec
sorting block 212, #reads = 1080084 mem = 536879531 peak = 1300226048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285179, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.25, 135.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674988 sequences (100000280 bp) in 2.683 CPU sec, 3.587 real sec...
[M::process] read 675414 sequences (100000199 bp) in 2.073 CPU sec, 2.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285205, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.15, 132.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 61, 98)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172)
[M::mem_pestat] mean and std.dev: (63.12, 28.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 209)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675006 reads in 252.775 CPU sec, 8.283 real sec
[M::mem_process_seqs] Processed 674850 reads in 141.346 CPU sec, 4.589 real sec
sorting block 213, #reads = 1079912 mem = 536878127 peak = 1300226048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285334, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.13, 128.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675512 sequences (100000247 bp) in 2.433 CPU sec, 3.134 real sec...
[M::process] read 675466 sequences (100000073 bp) in 2.103 CPU sec, 2.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285962, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.84, 121.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674988 reads in 248.670 CPU sec, 8.096 real sec
[M::mem_process_seqs] Processed 675414 reads in 139.381 CPU sec, 4.489 real sec
sorting block 214, #reads = 1080434 mem = 536878021 peak = 1300226048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286004, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.10, 122.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675602 sequences (100000035 bp) in 2.507 CPU sec, 3.230 real sec...
[M::process] read 675366 sequences (100000118 bp) in 1.968 CPU sec, 1.975 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286179, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (246.10, 122.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675512 reads in 241.958 CPU sec, 7.875 real sec
[M::mem_process_seqs] Processed 675466 reads in 136.127 CPU sec, 4.364 real sec
sorting block 215, #reads = 1081043 mem = 536878709 peak = 1300226048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285577, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (246.19, 124.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 18, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (76.78, 93.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675318 sequences (100000289 bp) in 2.510 CPU sec, 3.039 real sec...
[M::process] read 675252 sequences (100000217 bp) in 2.043 CPU sec, 2.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285666, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (247.27, 125.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675602 reads in 249.803 CPU sec, 8.104 real sec
sorting block 216, #reads = 1080716 mem = 536877520 peak = 1300226048
[M::mem_process_seqs] Processed 675366 reads in 142.731 CPU sec, 4.562 real sec
sorting block 217, #reads = 1080115 mem = 536878723 peak = 1300553728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285494, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (246.55, 123.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 1166, 1166)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3472)
[M::mem_pestat] mean and std.dev: (980.70, 1108.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5415)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675352 sequences (100000101 bp) in 2.670 CPU sec, 3.329 real sec...
[M::process] read 675246 sequences (100000261 bp) in 2.003 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285743, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (246.46, 122.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675318 reads in 249.906 CPU sec, 8.192 real sec
[M::mem_process_seqs] Processed 675252 reads in 138.850 CPU sec, 4.502 real sec
sorting block 218, #reads = 1080616 mem = 536881824 peak = 1300553728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286167, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.12, 122.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675136 sequences (100000005 bp) in 2.483 CPU sec, 2.992 real sec...
[M::process] read 675136 sequences (100000072 bp) in 2.015 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286382, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.25, 125.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 231, 752)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2234)
[M::mem_pestat] mean and std.dev: (346.85, 466.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2975)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675352 reads in 245.196 CPU sec, 7.981 real sec
[M::mem_process_seqs] Processed 675246 reads in 138.461 CPU sec, 4.452 real sec
sorting block 219, #reads = 1080707 mem = 536877539 peak = 1300553728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285591, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.04, 126.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 542, 1764)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5242)
[M::mem_pestat] mean and std.dev: (749.44, 745.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6981)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675170 sequences (100000162 bp) in 2.519 CPU sec, 3.335 real sec...
[M::process] read 675214 sequences (100000140 bp) in 1.968 CPU sec, 1.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285415, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.50, 127.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 372, 1878)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5628)
[M::mem_pestat] mean and std.dev: (790.10, 894.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7503)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675136 reads in 251.787 CPU sec, 8.183 real sec
[M::mem_process_seqs] Processed 675136 reads in 141.355 CPU sec, 4.524 real sec
sorting block 220, #reads = 1081019 mem = 536878267 peak = 1300553728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285354, 21, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.96, 131.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 352, 1741)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5205)
[M::mem_pestat] mean and std.dev: (676.90, 813.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6937)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 221, #reads = 1080536 mem = 536881611 peak = 1300553728
[M::process] read 675126 sequences (100000040 bp) in 2.482 CPU sec, 3.269 real sec...
[M::process] read 675064 sequences (100000131 bp) in 2.036 CPU sec, 2.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285479, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.32, 130.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675170 reads in 253.633 CPU sec, 8.315 real sec
[M::process] read 675124 sequences (100000045 bp) in 2.387 CPU sec, 2.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285575, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.02, 129.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675126 reads in 133.522 CPU sec, 4.253 real sec
sorting block 222, #reads = 1080337 mem = 536878035 peak = 1300553728
[M::mem_process_seqs] Processed 675214 reads in 331.305 CPU sec, 10.714 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285448, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.08, 130.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 70, 1318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3926)
[M::mem_pestat] mean and std.dev: (573.38, 695.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5230)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675144 sequences (100000193 bp) in 2.550 CPU sec, 3.142 real sec...
[M::process] read 675072 sequences (100000053 bp) in 2.011 CPU sec, 2.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285206, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.73, 132.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 21, 32)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 54)
[M::mem_pestat] mean and std.dev: (22.00, 6.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 65)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675064 reads in 266.493 CPU sec, 8.629 real sec
sorting block 223, #reads = 1080711 mem = 536880271 peak = 1300881408
[M::mem_process_seqs] Processed 675124 reads in 144.792 CPU sec, 4.629 real sec
sorting block 224, #reads = 1080583 mem = 536878277 peak = 1300881408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285655, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.89, 131.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675048 sequences (100000146 bp) in 2.743 CPU sec, 3.432 real sec...
[M::process] read 675054 sequences (100000273 bp) in 2.070 CPU sec, 2.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285612, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.94, 132.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675144 reads in 253.726 CPU sec, 8.289 real sec
[M::mem_process_seqs] Processed 675072 reads in 142.219 CPU sec, 4.616 real sec
sorting block 225, #reads = 1080759 mem = 536879224 peak = 1300881408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285784, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.85, 132.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675034 sequences (100000189 bp) in 2.513 CPU sec, 3.269 real sec...
[M::process] read 675030 sequences (100000112 bp) in 2.076 CPU sec, 2.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285512, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.92, 133.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 3, 5)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13)
[M::mem_pestat] mean and std.dev: (2.38, 1.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675048 reads in 251.498 CPU sec, 8.188 real sec
[M::mem_process_seqs] Processed 675054 reads in 141.744 CPU sec, 4.569 real sec
sorting block 226, #reads = 1080784 mem = 536878493 peak = 1300881408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285166, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.77, 133.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675018 sequences (100000147 bp) in 2.504 CPU sec, 3.357 real sec...
[M::process] read 674914 sequences (100000248 bp) in 2.109 CPU sec, 2.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285339, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.86, 134.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675034 reads in 255.059 CPU sec, 8.269 real sec
[M::mem_process_seqs] Processed 675030 reads in 142.140 CPU sec, 4.531 real sec
sorting block 227, #reads = 1080332 mem = 536876463 peak = 1300983808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285705, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.37, 134.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (833, 857, 917)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (665, 1085)
[M::mem_pestat] mean and std.dev: (913.00, 69.69)
[M::mem_pestat] low and high boundaries for proper pairs: (581, 1192)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674822 sequences (100000250 bp) in 2.577 CPU sec, 3.101 real sec...
[M::process] read 674744 sequences (100000138 bp) in 1.943 CPU sec, 1.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285381, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.81, 135.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 183, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (104.60, 132.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675018 reads in 254.142 CPU sec, 8.251 real sec
sorting block 228, #reads = 1080731 mem = 536880105 peak = 1300983808
[M::mem_process_seqs] Processed 674914 reads in 144.559 CPU sec, 4.617 real sec
sorting block 229, #reads = 1080949 mem = 536880832 peak = 1300983808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285641, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (260.63, 136.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (70, 225, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (171.58, 128.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674830 sequences (100000075 bp) in 2.647 CPU sec, 3.331 real sec...
[M::process] read 674978 sequences (100000086 bp) in 1.943 CPU sec, 2.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285748, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (260.93, 136.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674822 reads in 255.261 CPU sec, 8.338 real sec
[M::mem_process_seqs] Processed 674744 reads in 142.496 CPU sec, 4.622 real sec
sorting block 230, #reads = 1080459 mem = 536879718 peak = 1300983808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285192, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.07, 135.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 13, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302)
[M::mem_pestat] mean and std.dev: (25.50, 34.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 401)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674910 sequences (100000069 bp) in 2.475 CPU sec, 3.166 real sec...
[M::process] read 674798 sequences (100000271 bp) in 1.978 CPU sec, 2.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285781, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.38, 135.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 121, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (188.33, 203.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674830 reads in 254.517 CPU sec, 8.281 real sec
[M::mem_process_seqs] Processed 674978 reads in 142.280 CPU sec, 4.583 real sec
sorting block 231, #reads = 1080741 mem = 536878629 peak = 1300983808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285408, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (260.50, 136.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674942 sequences (100000193 bp) in 2.475 CPU sec, 3.101 real sec...
[M::process] read 674978 sequences (100000288 bp) in 2.092 CPU sec, 2.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285130, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (261.81, 138.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674910 reads in 254.263 CPU sec, 8.263 real sec
[M::mem_process_seqs] Processed 674798 reads in 143.471 CPU sec, 4.586 real sec
sorting block 232, #reads = 1081236 mem = 536878459 peak = 1300983808
[M::process] read 558416 sequences (82730889 bp) in 2.178 CPU sec, 2.691 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285392, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (261.40, 138.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285033, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (260.77, 138.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 33, 60)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 152)
[M::mem_pestat] mean and std.dev: (34.00, 27.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 198)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674942 reads in 248.005 CPU sec, 8.108 real sec
sorting block 233, #reads = 1080520 mem = 536878810 peak = 1300983808
[M::mem_process_seqs] Processed 674978 reads in 139.761 CPU sec, 4.532 real sec
sorting block 234, #reads = 1080293 mem = 536881543 peak = 1300983808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 236486, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (262.06, 140.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 558416 reads in 104.374 CPU sec, 3.539 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050654
  features: 1f8bfbff fffa3203
  extended: d19f47ab 00000018
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8175M CPU @ 2.50GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 252340328
overall: 69925064704 mem 50505.946 user 297.351 sys 1671.583 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003322.L005\tPU:L005\tSM:T21-D0003322\tLB:T21-D0003322\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 T21-D0003322_R1.fastq.gz T21-D0003322_R2.fastq.gz
[main] Real time: 1671.608 sec; CPU: 50803.300 sec
sorting block 235, #reads = 831036 mem = 413115410 peak = 1300983808
uniq header count 1
merging 236 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050654
  features: 1f8bfbff fffa3203
  extended: d19f47ab 00000018
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8175M CPU @ 2.50GHz
threads: 31 max 32
algo: util-sort
license: sentieon:util=1
output file size: 8497222000
output reads: 254727155
bam_mem_sort: 236 calls 241.911 user 0.053 sys 254.997 real
bam_write: 236 calls 162.213 user 10.542 sys 455.000 real
execute: 1 calls 84.411 user 2.633 sys 1765.096 real
merge_files: 1 calls 84.379 user 2.592 sys 92.877 real
parse_chunk: 3773 calls 329.411 user 65.153 sys 520.735 real
read_chunk: 10153 calls 164.972 user 77.485 sys 1664.805 real
sort_block: 1 calls 111.964 user 76.548 sys 1602.026 real
write_chunk: 6378 calls 83.650 user 2.287 sys 89.216 real
overall: 3020173312 mem 3890.154 user 164.381 sys 1765.116 real
ls: cannot access '*.cram': No such file or directory