Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5b/47dac41942ca3c98da2cf8107c6a81/T18-D0003255/0003.T18-D0003255_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/8b/ad780026d4ee1373c3a92f6a7789e2/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5b/47dac41942ca3c98da2cf8107c6a81/T18-D0003255/0003.T18-D0003255_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/8b/ad780026d4ee1373c3a92f6a7789e2/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
Received only 10 licenses out of 31 requested for sentieon:klib
Received only 10 licenses out of 31 requested for sentieon:klib
Received only 10 licenses out of 31 requested for sentieon:klib
Received only 10 licenses out of 31 requested for sentieon:klib
[M::main_mem] bwa index loaded in 1906.208 CPU sec, 376.942 real sec
[M::process] read 673470 sequences (100000132 bp) in 0.967 CPU sec, 0.976 real sec...
[M::process] read 673148 sequences (100000006 bp) in 1.040 CPU sec, 1.032 real sec...
[M::process] read 672992 sequences (100000090 bp) in 1.031 CPU sec, 1.034 real sec...
[M::process] read 672974 sequences (100000036 bp) in 1.080 CPU sec, 1.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286209, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (262.56, 131.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 346, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1769)
[M::mem_pestat] mean and std.dev: (342.00, 320.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2349)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286058, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.66, 136.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673470 reads in 177.075 CPU sec, 17.732 real sec
[M::mem_process_seqs] Processed 673148 reads in 93.020 CPU sec, 9.557 real sec
sorting block 0, #reads = 1080362 mem = 536879735 peak = 596799488
[M::process] read 672938 sequences (100000247 bp) in 1.454 CPU sec, 1.519 real sec...
[M::process] read 672610 sequences (100000010 bp) in 1.303 CPU sec, 1.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285802, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.51, 138.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (317, 369, 9729)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28553)
[M::mem_pestat] mean and std.dev: (2930.91, 4181.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 37965)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285236, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.79, 139.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672992 reads in 178.054 CPU sec, 17.655 real sec
[M::mem_process_seqs] Processed 672974 reads in 184.537 CPU sec, 18.337 real sec
sorting block 1, #reads = 1079363 mem = 536877942 peak = 716337152
[M::process] read 672490 sequences (100000116 bp) in 1.896 CPU sec, 2.065 real sec...
[M::process] read 672546 sequences (100000128 bp) in 0.976 CPU sec, 0.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285362, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.59, 144.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284928, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (281.94, 146.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672938 reads in 229.500 CPU sec, 11.128 real sec
[M::mem_process_seqs] Processed 672610 reads in 238.835 CPU sec, 11.445 real sec
sorting block 2, #reads = 1079637 mem = 536882134 peak = 746799104
[M::process] read 672690 sequences (100000095 bp) in 1.840 CPU sec, 1.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285223, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 210, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (287.45, 151.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (92, 569, 1213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3455)
[M::mem_pestat] mean and std.dev: (675.45, 622.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4576)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672864 sequences (100000193 bp) in 1.190 CPU sec, 1.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285140, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (287.44, 151.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284946, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.16, 147.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 69, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513)
[M::mem_pestat] mean and std.dev: (267.15, 366.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2013)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672490 reads in 317.013 CPU sec, 10.239 real sec
[M::mem_process_seqs] Processed 672546 reads in 222.350 CPU sec, 7.223 real sec
sorting block 3, #reads = 1079950 mem = 536879376 peak = 790904832
[M::mem_process_seqs] Processed 672690 reads in 231.180 CPU sec, 7.540 real sec
sorting block 4, #reads = 1079135 mem = 536882178 peak = 876425216
[M::process] read 673240 sequences (100000031 bp) in 2.091 CPU sec, 2.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285175, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.47, 145.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673070 sequences (100000139 bp) in 1.716 CPU sec, 1.772 real sec...
[M::process] read 673050 sequences (100000152 bp) in 1.220 CPU sec, 1.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285194, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.92, 136.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672864 reads in 213.532 CPU sec, 6.830 real sec
[M::mem_process_seqs] Processed 673240 reads in 117.327 CPU sec, 3.767 real sec
sorting block 5, #reads = 1079088 mem = 536882733 peak = 876425216
[M::process] read 673006 sequences (100000116 bp) in 2.004 CPU sec, 2.142 real sec...
[M::process] read 672816 sequences (100000056 bp) in 1.460 CPU sec, 1.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285190, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.18, 142.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673006 reads in 106.887 CPU sec, 3.438 real sec
sorting block 6, #reads = 1080239 mem = 536878017 peak = 876425216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285433, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.37, 138.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672756 sequences (100000072 bp) in 1.585 CPU sec, 1.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285527, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.77, 140.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673070 reads in 315.748 CPU sec, 10.104 real sec
[M::mem_process_seqs] Processed 673050 reads in 222.585 CPU sec, 7.167 real sec
sorting block 7, #reads = 1079813 mem = 536880981 peak = 876425216
[M::process] read 672912 sequences (100000003 bp) in 1.663 CPU sec, 1.757 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284835, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.50, 144.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 35, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742)
[M::mem_pestat] mean and std.dev: (373.50, 574.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2670)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673092 sequences (100000118 bp) in 1.340 CPU sec, 1.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285173, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.67, 146.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285353, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.04, 144.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672816 reads in 296.652 CPU sec, 9.539 real sec
sorting block 8, #reads = 1079928 mem = 536880132 peak = 876425216
[M::mem_process_seqs] Processed 672756 reads in 206.268 CPU sec, 6.689 real sec
[M::mem_process_seqs] Processed 672912 reads in 215.228 CPU sec, 7.003 real sec
sorting block 9, #reads = 1079221 mem = 536878827 peak = 876425216
[M::process] read 672996 sequences (100000181 bp) in 1.907 CPU sec, 2.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285261, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (266.42, 133.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 425, 1060)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3022)
[M::mem_pestat] mean and std.dev: (459.40, 597.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4003)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673014 sequences (100000282 bp) in 1.406 CPU sec, 1.479 real sec...
[M::process] read 672848 sequences (100000174 bp) in 0.990 CPU sec, 0.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285522, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.80, 138.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 169, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (173.78, 149.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673092 reads in 199.195 CPU sec, 6.387 real sec
[M::mem_process_seqs] Processed 672996 reads in 108.229 CPU sec, 3.474 real sec
sorting block 10, #reads = 1079358 mem = 536876814 peak = 876425216
[M::process] read 672828 sequences (100000194 bp) in 1.960 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285424, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.54, 140.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672744 sequences (100000002 bp) in 1.100 CPU sec, 1.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284683, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.58, 141.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 10, 15)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25)
[M::mem_pestat] mean and std.dev: (8.78, 4.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 30)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285097, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.05, 144.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 220, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1937)
[M::mem_pestat] mean and std.dev: (351.42, 522.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2581)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673014 reads in 303.346 CPU sec, 9.746 real sec
sorting block 11, #reads = 1079631 mem = 536877470 peak = 876425216
[M::mem_process_seqs] Processed 672848 reads in 213.691 CPU sec, 6.912 real sec
[M::mem_process_seqs] Processed 672828 reads in 222.733 CPU sec, 7.229 real sec
sorting block 12, #reads = 1079893 mem = 536878982 peak = 926396416
[M::process] read 672420 sequences (100000274 bp) in 2.592 CPU sec, 2.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284670, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.90, 149.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 13, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380)
[M::mem_pestat] mean and std.dev: (69.22, 103.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 505)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672594 sequences (100000201 bp) in 1.841 CPU sec, 1.916 real sec...
[M::process] read 672788 sequences (100000117 bp) in 1.530 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284293, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 212, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (289.77, 152.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672744 reads in 221.095 CPU sec, 7.070 real sec
[M::mem_process_seqs] Processed 672420 reads in 125.006 CPU sec, 3.984 real sec
sorting block 13, #reads = 1080099 mem = 536878456 peak = 926396416
sorting block 14, #reads = 1079651 mem = 536879949 peak = 926396416
[M::process] read 673010 sequences (100000277 bp) in 2.265 CPU sec, 2.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284423, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (288.58, 152.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673262 sequences (100000272 bp) in 1.570 CPU sec, 1.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284712, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.17, 143.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283963, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (284.11, 150.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 33, 4069)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12167)
[M::mem_pestat] mean and std.dev: (1834.82, 2566.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16216)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672594 reads in 321.047 CPU sec, 10.295 real sec
[M::mem_process_seqs] Processed 673010 reads in 221.353 CPU sec, 7.134 real sec
[M::mem_process_seqs] Processed 672788 reads in 229.299 CPU sec, 7.414 real sec
sorting block 15, #reads = 1079505 mem = 536877787 peak = 926396416
[M::process] read 673096 sequences (100000135 bp) in 2.298 CPU sec, 2.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285734, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.41, 133.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 68, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321)
[M::mem_pestat] mean and std.dev: (126.64, 159.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1751)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672990 sequences (100000017 bp) in 1.618 CPU sec, 1.637 real sec...
[M::process] read 672986 sequences (100000110 bp) in 1.490 CPU sec, 1.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285137, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.31, 141.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673262 reads in 204.540 CPU sec, 6.532 real sec
sorting block 16, #reads = 1079636 mem = 536880280 peak = 926396416
[M::mem_process_seqs] Processed 673096 reads in 115.280 CPU sec, 3.668 real sec
sorting block 17, #reads = 1080045 mem = 536880390 peak = 929275904
[M::process] read 672816 sequences (100000212 bp) in 2.323 CPU sec, 2.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284954, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (273.16, 142.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672668 sequences (100000247 bp) in 1.200 CPU sec, 1.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284706, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.96, 144.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285010, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.60, 146.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 369, 1528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4506)
[M::mem_pestat] mean and std.dev: (768.13, 1025.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5995)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672990 reads in 308.408 CPU sec, 9.892 real sec
[M::mem_process_seqs] Processed 672986 reads in 213.168 CPU sec, 6.889 real sec
[M::mem_process_seqs] Processed 672816 reads in 222.615 CPU sec, 7.227 real sec
sorting block 18, #reads = 1079553 mem = 536878380 peak = 929275904
[M::process] read 672880 sequences (100000152 bp) in 2.522 CPU sec, 2.846 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285169, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.86, 148.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 96, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (113.89, 133.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 19, #reads = 1079532 mem = 536879264 peak = 929275904
[M::process] read 673232 sequences (100000129 bp) in 1.659 CPU sec, 1.708 real sec...
[M::process] read 673132 sequences (100000009 bp) in 1.510 CPU sec, 1.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285719, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.66, 140.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 25, 88)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214)
[M::mem_pestat] mean and std.dev: (34.36, 26.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 277)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672668 reads in 202.120 CPU sec, 6.447 real sec
[M::mem_process_seqs] Processed 672880 reads in 109.858 CPU sec, 3.492 real sec
sorting block 20, #reads = 1078896 mem = 536879679 peak = 929275904
[M::process] read 673116 sequences (100000021 bp) in 2.152 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285374, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (268.26, 135.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673112 sequences (100000214 bp) in 1.470 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285402, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.64, 136.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285649, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (268.19, 135.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 224, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1466)
[M::mem_pestat] mean and std.dev: (237.31, 181.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1926)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673232 reads in 276.904 CPU sec, 8.876 real sec
[M::mem_process_seqs] Processed 673132 reads in 193.421 CPU sec, 6.229 real sec
[M::mem_process_seqs] Processed 673116 reads in 202.276 CPU sec, 6.545 real sec
sorting block 21, #reads = 1079929 mem = 536878113 peak = 929275904
[M::process] read 673002 sequences (100000080 bp) in 1.988 CPU sec, 2.218 real sec...
sorting block 22, #reads = 1079811 mem = 536878758 peak = 929275904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285634, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.88, 137.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 574, 1411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3909)
[M::mem_pestat] mean and std.dev: (583.54, 578.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5158)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672782 sequences (100000290 bp) in 1.845 CPU sec, 1.964 real sec...
[M::process] read 672792 sequences (100000115 bp) in 1.300 CPU sec, 1.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284935, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (274.05, 140.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673112 reads in 204.356 CPU sec, 6.535 real sec
[M::mem_process_seqs] Processed 673002 reads in 112.706 CPU sec, 3.596 real sec
sorting block 23, #reads = 1080354 mem = 536883439 peak = 929275904
[M::process] read 672694 sequences (100000288 bp) in 2.163 CPU sec, 2.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284884, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (280.15, 145.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672740 sequences (100000286 bp) in 1.539 CPU sec, 1.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285301, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.72, 147.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284658, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (283.51, 148.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 23, 94)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276)
[M::mem_pestat] mean and std.dev: (26.36, 32.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 367)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672782 reads in 300.639 CPU sec, 9.642 real sec
[M::mem_process_seqs] Processed 672792 reads in 211.115 CPU sec, 6.803 real sec
[M::mem_process_seqs] Processed 672694 reads in 219.718 CPU sec, 7.108 real sec
sorting block 24, #reads = 1079695 mem = 536879896 peak = 929275904
sorting block 25, #reads = 1080136 mem = 536877141 peak = 929275904
[M::process] read 673112 sequences (100000059 bp) in 2.850 CPU sec, 3.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284260, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.09, 147.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 130, 176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480)
[M::mem_pestat] mean and std.dev: (91.27, 74.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673216 sequences (100000216 bp) in 1.741 CPU sec, 1.783 real sec...
[M::process] read 672948 sequences (100000111 bp) in 1.530 CPU sec, 1.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285401, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.38, 137.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 194, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (277.60, 231.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1394)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672740 reads in 212.167 CPU sec, 6.772 real sec
[M::mem_process_seqs] Processed 673112 reads in 117.325 CPU sec, 3.730 real sec
sorting block 26, #reads = 1079293 mem = 536878232 peak = 929275904
[M::process] read 673042 sequences (100000072 bp) in 2.208 CPU sec, 2.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285224, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.29, 134.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672794 sequences (100000148 bp) in 1.410 CPU sec, 1.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284679, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (275.22, 143.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 122, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (72.91, 86.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284473, 16, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.43, 141.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 48, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428)
[M::mem_pestat] mean and std.dev: (88.62, 105.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673216 reads in 274.675 CPU sec, 8.803 real sec
[M::mem_process_seqs] Processed 673042 reads in 185.702 CPU sec, 5.992 real sec
sorting block 27, #reads = 1080262 mem = 536879145 peak = 929275904
[M::mem_process_seqs] Processed 672948 reads in 193.369 CPU sec, 6.267 real sec
sorting block 28, #reads = 1080025 mem = 536880122 peak = 929275904
[M::process] read 672818 sequences (100000273 bp) in 2.071 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284330, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.38, 145.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672860 sequences (100000082 bp) in 1.309 CPU sec, 1.369 real sec...
[M::process] read 673308 sequences (100000084 bp) in 1.140 CPU sec, 1.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284198, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.35, 146.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 46, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102)
[M::mem_pestat] mean and std.dev: (157.50, 166.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1465)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672794 reads in 180.778 CPU sec, 5.799 real sec
[M::mem_process_seqs] Processed 672818 reads in 100.747 CPU sec, 3.218 real sec
sorting block 29, #reads = 1079356 mem = 536879583 peak = 929275904
[M::process] read 673254 sequences (100000249 bp) in 2.324 CPU sec, 2.562 real sec...
[M::process] read 673666 sequences (100000011 bp) in 1.140 CPU sec, 1.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285981, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.39, 133.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673308 reads in 93.893 CPU sec, 3.010 real sec
sorting block 30, #reads = 1079432 mem = 536877608 peak = 929275904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284530, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.17, 147.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673424 sequences (100000050 bp) in 1.309 CPU sec, 1.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285275, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (262.56, 131.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 180, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (100.38, 90.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672860 reads in 278.008 CPU sec, 8.903 real sec
[M::mem_process_seqs] Processed 673254 reads in 114.140 CPU sec, 3.704 real sec
sorting block 31, #reads = 1079337 mem = 536878097 peak = 929275904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285627, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (258.11, 127.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673262 sequences (100000270 bp) in 2.458 CPU sec, 2.699 real sec...
[M::process] read 673146 sequences (100000043 bp) in 1.540 CPU sec, 1.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285820, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (261.03, 129.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673666 reads in 189.436 CPU sec, 6.046 real sec
[M::mem_process_seqs] Processed 673424 reads in 105.867 CPU sec, 3.394 real sec
sorting block 32, #reads = 1080560 mem = 536879617 peak = 929275904
[M::process] read 672828 sequences (100000244 bp) in 1.797 CPU sec, 1.958 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284870, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (265.40, 132.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672886 sequences (100000240 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285300, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.35, 138.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285130, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.95, 139.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673262 reads in 267.100 CPU sec, 8.571 real sec
sorting block 33, #reads = 1080290 mem = 536879273 peak = 929275904
[M::mem_process_seqs] Processed 673146 reads in 177.814 CPU sec, 5.743 real sec
[M::mem_process_seqs] Processed 672828 reads in 185.339 CPU sec, 6.011 real sec
sorting block 34, #reads = 1080112 mem = 536879984 peak = 929275904
[M::process] read 673020 sequences (100000250 bp) in 1.994 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284680, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (274.76, 140.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673064 sequences (100000190 bp) in 1.242 CPU sec, 1.288 real sec...
[M::process] read 673368 sequences (100000011 bp) in 1.070 CPU sec, 1.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285148, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.53, 139.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672886 reads in 173.820 CPU sec, 5.564 real sec
[M::mem_process_seqs] Processed 673020 reads in 95.887 CPU sec, 3.067 real sec
sorting block 35, #reads = 1079982 mem = 536878091 peak = 929275904
sorting block 36, #reads = 1078935 mem = 536878682 peak = 929275904
[M::process] read 673118 sequences (100000184 bp) in 1.818 CPU sec, 2.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284789, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.54, 137.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 130, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (193.07, 199.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672972 sequences (100000181 bp) in 1.060 CPU sec, 1.059 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285026, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.86, 135.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285451, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.62, 133.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 16, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177)
[M::mem_pestat] mean and std.dev: (23.10, 29.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 235)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673064 reads in 248.193 CPU sec, 7.967 real sec
[M::mem_process_seqs] Processed 673118 reads in 172.309 CPU sec, 5.570 real sec
[M::mem_process_seqs] Processed 673368 reads in 179.943 CPU sec, 5.846 real sec
sorting block 37, #reads = 1079937 mem = 536880413 peak = 929275904
[M::process] read 672896 sequences (100000227 bp) in 1.940 CPU sec, 2.243 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285332, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.19, 139.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672812 sequences (100000287 bp) in 1.176 CPU sec, 1.214 real sec...
[M::process] read 672618 sequences (100000010 bp) in 1.100 CPU sec, 1.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284616, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.37, 142.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672972 reads in 174.012 CPU sec, 5.574 real sec
sorting block 38, #reads = 1079638 mem = 536880597 peak = 929275904
[M::mem_process_seqs] Processed 672896 reads in 96.659 CPU sec, 3.091 real sec
sorting block 39, #reads = 1079837 mem = 536880306 peak = 929275904
[M::process] read 672654 sequences (100000283 bp) in 1.865 CPU sec, 2.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284868, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.53, 145.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673412 sequences (100000257 bp) in 1.070 CPU sec, 1.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284363, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.90, 147.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284854, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 204, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (281.90, 147.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672812 reads in 251.562 CPU sec, 8.080 real sec
[M::mem_process_seqs] Processed 672654 reads in 175.234 CPU sec, 5.652 real sec
[M::mem_process_seqs] Processed 672618 reads in 182.708 CPU sec, 5.919 real sec
sorting block 40, #reads = 1079521 mem = 536878532 peak = 929275904
[M::process] read 673230 sequences (100000174 bp) in 2.096 CPU sec, 2.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285273, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.11, 138.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1079276 mem = 536879070 peak = 929275904
[M::process] read 673204 sequences (100000065 bp) in 1.223 CPU sec, 1.270 real sec...
[M::process] read 673176 sequences (100000295 bp) in 1.100 CPU sec, 1.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284439, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (273.35, 141.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673412 reads in 173.818 CPU sec, 5.576 real sec
[M::mem_process_seqs] Processed 673230 reads in 98.085 CPU sec, 3.136 real sec
sorting block 42, #reads = 1079693 mem = 536879042 peak = 929275904
[M::process] read 672938 sequences (100000100 bp) in 1.805 CPU sec, 2.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284487, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.59, 141.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672738 sequences (100000155 bp) in 1.070 CPU sec, 1.068 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284241, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.67, 145.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284794, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.05, 143.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673204 reads in 255.996 CPU sec, 8.225 real sec
[M::mem_process_seqs] Processed 672938 reads in 179.450 CPU sec, 5.795 real sec
[M::mem_process_seqs] Processed 673176 reads in 186.796 CPU sec, 6.061 real sec
sorting block 43, #reads = 1079337 mem = 536880081 peak = 929275904
[M::process] read 672646 sequences (100000100 bp) in 2.035 CPU sec, 2.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284071, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 44, #reads = 1079295 mem = 536881822 peak = 929275904
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.23, 150.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672584 sequences (100000076 bp) in 1.315 CPU sec, 1.394 real sec...
[M::process] read 672760 sequences (100000162 bp) in 1.080 CPU sec, 1.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284002, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (288.79, 153.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672738 reads in 182.721 CPU sec, 5.865 real sec
[M::mem_process_seqs] Processed 672646 reads in 102.138 CPU sec, 3.268 real sec
sorting block 45, #reads = 1079004 mem = 536878977 peak = 929275904
[M::process] read 672944 sequences (100000058 bp) in 2.191 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283530, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (286.03, 151.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 18, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288)
[M::mem_pestat] mean and std.dev: (48.33, 63.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 381)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673462 sequences (100000159 bp) in 1.060 CPU sec, 1.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284177, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.15, 150.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284576, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.78, 144.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672584 reads in 259.918 CPU sec, 8.339 real sec
[M::mem_process_seqs] Processed 672760 reads in 179.267 CPU sec, 5.784 real sec
[M::mem_process_seqs] Processed 672944 reads in 186.666 CPU sec, 6.048 real sec
sorting block 46, #reads = 1079242 mem = 536878873 peak = 929275904
[M::process] read 673236 sequences (100000041 bp) in 1.901 CPU sec, 2.186 real sec...
sorting block 47, #reads = 1079403 mem = 536879795 peak = 929275904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284681, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.25, 136.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673278 sequences (100000184 bp) in 1.668 CPU sec, 1.800 real sec...
[M::process] read 673148 sequences (100000014 bp) in 1.180 CPU sec, 1.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284920, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.85, 140.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (97, 248, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (283.27, 225.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673462 reads in 179.709 CPU sec, 5.747 real sec
[M::mem_process_seqs] Processed 673236 reads in 99.567 CPU sec, 3.173 real sec
sorting block 48, #reads = 1079779 mem = 536879801 peak = 929275904
[M::process] read 673058 sequences (100000165 bp) in 1.744 CPU sec, 1.921 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284989, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.48, 140.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 49, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2160)
[M::mem_pestat] mean and std.dev: (409.91, 607.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2874)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672976 sequences (100000104 bp) in 1.110 CPU sec, 1.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284956, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.90, 146.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284459, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.54, 144.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 252, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (294.27, 229.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673278 reads in 256.317 CPU sec, 8.230 real sec
[M::mem_process_seqs] Processed 673058 reads in 178.640 CPU sec, 5.774 real sec
[M::mem_process_seqs] Processed 673148 reads in 186.903 CPU sec, 6.073 real sec
sorting block 49, #reads = 1079767 mem = 536879138 peak = 929275904
sorting block 50, #reads = 1079608 mem = 536883461 peak = 929275904
[M::process] read 673262 sequences (100000085 bp) in 2.197 CPU sec, 2.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284593, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (276.62, 148.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673054 sequences (100000110 bp) in 1.384 CPU sec, 1.445 real sec...
[M::process] read 673140 sequences (100000111 bp) in 1.170 CPU sec, 1.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284442, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.82, 140.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 79, 174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486)
[M::mem_pestat] mean and std.dev: (66.54, 61.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672976 reads in 175.958 CPU sec, 5.643 real sec
[M::mem_process_seqs] Processed 673262 reads in 101.024 CPU sec, 3.235 real sec
sorting block 51, #reads = 1079256 mem = 536876604 peak = 929275904
[M::process] read 673180 sequences (100000202 bp) in 1.867 CPU sec, 2.069 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284458, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.78, 141.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 25, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (42.53, 47.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 490)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673056 sequences (100000272 bp) in 1.090 CPU sec, 1.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284708, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.98, 140.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 33, 46)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 130)
[M::mem_pestat] mean and std.dev: (26.50, 26.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 172)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284936, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.51, 139.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 14, 92)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250)
[M::mem_pestat] mean and std.dev: (36.92, 38.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 329)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673054 reads in 254.093 CPU sec, 8.155 real sec
[M::mem_process_seqs] Processed 673140 reads in 176.600 CPU sec, 5.705 real sec
sorting block 52, #reads = 1079547 mem = 536879491 peak = 929275904
[M::mem_process_seqs] Processed 673180 reads in 183.974 CPU sec, 5.971 real sec
sorting block 53, #reads = 1079433 mem = 536877082 peak = 929275904
[M::process] read 672936 sequences (100000040 bp) in 1.895 CPU sec, 2.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284236, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.86, 142.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (92, 92, 94)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (88, 98)
[M::mem_pestat] mean and std.dev: (92.33, 0.75)
[M::mem_pestat] low and high boundaries for proper pairs: (86, 100)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672634 sequences (100000270 bp) in 1.430 CPU sec, 1.537 real sec...
[M::process] read 672708 sequences (100000127 bp) in 1.140 CPU sec, 1.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283608, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.92, 148.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673056 reads in 182.677 CPU sec, 5.847 real sec
[M::mem_process_seqs] Processed 672936 reads in 102.596 CPU sec, 3.286 real sec
sorting block 54, #reads = 1079486 mem = 536878458 peak = 929275904
[M::process] read 672922 sequences (100000266 bp) in 1.734 CPU sec, 1.919 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283356, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (287.74, 153.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673258 sequences (100000072 bp) in 1.110 CPU sec, 1.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283667, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (286.62, 152.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283886, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (280.97, 149.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672634 reads in 268.868 CPU sec, 8.636 real sec
[M::mem_process_seqs] Processed 672708 reads in 188.407 CPU sec, 6.103 real sec
sorting block 55, #reads = 1079476 mem = 536880934 peak = 929275904
[M::mem_process_seqs] Processed 672922 reads in 196.927 CPU sec, 6.409 real sec
sorting block 56, #reads = 1078854 mem = 536882673 peak = 967663616
[M::process] read 673802 sequences (100000217 bp) in 1.946 CPU sec, 2.253 real sec...
[M::process] read 673416 sequences (100000063 bp) in 1.364 CPU sec, 1.465 real sec...
[M::process] read 673198 sequences (100000241 bp) in 1.110 CPU sec, 1.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285074, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.35, 130.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673802 reads in 94.701 CPU sec, 3.011 real sec
sorting block 57, #reads = 1079721 mem = 536878297 peak = 967663616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284248, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.22, 143.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 50, 161)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407)
[M::mem_pestat] mean and std.dev: (79.18, 75.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 530)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673174 sequences (100000063 bp) in 1.176 CPU sec, 1.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284544, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.68, 140.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673258 reads in 278.883 CPU sec, 8.925 real sec
[M::mem_process_seqs] Processed 673416 reads in 110.520 CPU sec, 3.580 real sec
sorting block 58, #reads = 1079979 mem = 536880590 peak = 967663616
[M::process] read 673014 sequences (100000152 bp) in 1.743 CPU sec, 1.938 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283902, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.24, 142.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673172 sequences (100000194 bp) in 1.110 CPU sec, 1.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284272, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.93, 147.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284535, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.95, 144.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 75, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127)
[M::mem_pestat] mean and std.dev: (145.69, 142.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1498)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673198 reads in 262.647 CPU sec, 8.440 real sec
[M::mem_process_seqs] Processed 673014 reads in 182.081 CPU sec, 5.884 real sec
sorting block 59, #reads = 1079737 mem = 536880198 peak = 967663616
[M::mem_process_seqs] Processed 673174 reads in 190.179 CPU sec, 6.176 real sec
sorting block 60, #reads = 1079329 mem = 536878057 peak = 967663616
[M::process] read 673418 sequences (100000119 bp) in 2.139 CPU sec, 2.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284303, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.02, 148.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673288 sequences (100000160 bp) in 1.284 CPU sec, 1.379 real sec...
[M::process] read 673240 sequences (100000100 bp) in 1.080 CPU sec, 1.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284524, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.57, 137.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673172 reads in 178.682 CPU sec, 5.836 real sec
[M::mem_process_seqs] Processed 673418 reads in 100.035 CPU sec, 3.200 real sec
sorting block 61, #reads = 1079012 mem = 536877551 peak = 967663616
[M::process] read 673348 sequences (100000005 bp) in 1.859 CPU sec, 2.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284740, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.98, 139.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673392 sequences (100000077 bp) in 1.100 CPU sec, 1.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284025, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.44, 135.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 87, 173)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471)
[M::mem_pestat] mean and std.dev: (85.76, 66.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284607, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.83, 136.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 128, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (126.50, 151.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 777)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673288 reads in 261.614 CPU sec, 8.403 real sec
[M::mem_process_seqs] Processed 673348 reads in 184.320 CPU sec, 5.954 real sec
sorting block 62, #reads = 1079081 mem = 536879477 peak = 967663616
[M::mem_process_seqs] Processed 673240 reads in 192.454 CPU sec, 6.245 real sec
sorting block 63, #reads = 1079187 mem = 536879409 peak = 967663616
[M::process] read 673154 sequences (100000052 bp) in 2.014 CPU sec, 2.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284090, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.94, 137.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672938 sequences (100000289 bp) in 1.351 CPU sec, 1.430 real sec...
[M::process] read 672826 sequences (100000035 bp) in 1.120 CPU sec, 1.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283843, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.39, 141.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673392 reads in 179.603 CPU sec, 5.764 real sec
[M::mem_process_seqs] Processed 673154 reads in 99.599 CPU sec, 3.187 real sec
sorting block 64, #reads = 1079296 mem = 536880770 peak = 967663616
[M::process] read 673038 sequences (100000128 bp) in 1.938 CPU sec, 2.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283991, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.64, 146.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673254 sequences (100000242 bp) in 1.090 CPU sec, 1.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283468, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.26, 149.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 28, 29)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 81)
[M::mem_pestat] mean and std.dev: (22.22, 16.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 107)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284223, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.19, 149.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672938 reads in 278.761 CPU sec, 9.066 real sec
sorting block 65, #reads = 1079237 mem = 536878117 peak = 967663616
[M::mem_process_seqs] Processed 672826 reads in 196.418 CPU sec, 6.359 real sec
[M::mem_process_seqs] Processed 673038 reads in 206.472 CPU sec, 6.721 real sec
sorting block 66, #reads = 1079456 mem = 536880502 peak = 967663616
[M::process] read 673596 sequences (100000149 bp) in 1.897 CPU sec, 2.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284243, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.25, 139.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673350 sequences (100000052 bp) in 1.595 CPU sec, 1.778 real sec...
[M::process] read 673204 sequences (100000225 bp) in 1.120 CPU sec, 1.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285085, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.75, 132.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 559, 595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1525)
[M::mem_pestat] mean and std.dev: (408.09, 300.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1990)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673254 reads in 188.320 CPU sec, 6.028 real sec
[M::mem_process_seqs] Processed 673596 reads in 106.594 CPU sec, 3.430 real sec
sorting block 67, #reads = 1079419 mem = 536878625 peak = 967663616
sorting block 68, #reads = 1079844 mem = 536876563 peak = 967663616
[M::process] read 673218 sequences (100000235 bp) in 1.781 CPU sec, 1.978 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285052, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.27, 141.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673250 sequences (100000124 bp) in 1.130 CPU sec, 1.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284654, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.22, 145.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284252, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.75, 141.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 130, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (97.91, 89.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673350 reads in 269.353 CPU sec, 8.659 real sec
[M::mem_process_seqs] Processed 673218 reads in 191.011 CPU sec, 6.188 real sec
[M::mem_process_seqs] Processed 673204 reads in 201.131 CPU sec, 6.554 real sec
sorting block 69, #reads = 1079839 mem = 536875343 peak = 967663616
[M::process] read 673070 sequences (100000146 bp) in 2.039 CPU sec, 2.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284920, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.63, 148.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673786 sequences (100000193 bp) in 1.178 CPU sec, 1.216 real sec...
[M::process] read 673454 sequences (100000012 bp) in 1.130 CPU sec, 1.126 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284451, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (276.51, 149.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (463, 1717, 3366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9172)
[M::mem_pestat] mean and std.dev: (1666.00, 1392.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12075)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673250 reads in 177.174 CPU sec, 5.684 real sec
sorting block 70, #reads = 1079353 mem = 536879365 peak = 967663616
[M::mem_process_seqs] Processed 673070 reads in 98.888 CPU sec, 3.169 real sec
sorting block 71, #reads = 1078623 mem = 536879741 peak = 967663616
[M::process] read 673558 sequences (100000287 bp) in 2.273 CPU sec, 2.540 real sec...
[M::process] read 673566 sequences (100000120 bp) in 1.190 CPU sec, 1.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285186, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.15, 135.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673454 reads in 90.844 CPU sec, 2.903 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285644, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.67, 137.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673374 sequences (100000146 bp) in 1.111 CPU sec, 1.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285705, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.15, 132.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673786 reads in 262.742 CPU sec, 8.411 real sec
[M::mem_process_seqs] Processed 673558 reads in 105.681 CPU sec, 3.433 real sec
sorting block 72, #reads = 1078780 mem = 536880291 peak = 967663616
sorting block 73, #reads = 1079628 mem = 536878257 peak = 967663616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284674, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 673174 sequences (100000012 bp) in 1.942 CPU sec, 2.245 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.75, 133.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673032 sequences (100000219 bp) in 1.410 CPU sec, 1.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284699, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.37, 135.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (67, 86, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141)
[M::mem_pestat] mean and std.dev: (176.10, 205.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1499)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673566 reads in 176.604 CPU sec, 5.645 real sec
[M::mem_process_seqs] Processed 673374 reads in 101.007 CPU sec, 3.243 real sec
sorting block 74, #reads = 1079445 mem = 536878747 peak = 967663616
[M::process] read 673124 sequences (100000113 bp) in 1.591 CPU sec, 1.720 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284342, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.88, 138.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673150 sequences (100000048 bp) in 1.080 CPU sec, 1.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283980, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (274.15, 143.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284034, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (274.18, 143.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673174 reads in 269.670 CPU sec, 8.685 real sec
[M::mem_process_seqs] Processed 673124 reads in 187.816 CPU sec, 6.083 real sec
[M::mem_process_seqs] Processed 673032 reads in 195.367 CPU sec, 6.354 real sec
sorting block 75, #reads = 1079396 mem = 536878907 peak = 967663616
[M::process] read 673336 sequences (100000131 bp) in 1.923 CPU sec, 2.228 real sec...
sorting block 76, #reads = 1079628 mem = 536877397 peak = 967663616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283852, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.51, 144.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673446 sequences (100000052 bp) in 1.355 CPU sec, 1.427 real sec...
[M::process] read 673266 sequences (100000163 bp) in 1.080 CPU sec, 1.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284856, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.88, 137.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673150 reads in 182.899 CPU sec, 5.863 real sec
[M::mem_process_seqs] Processed 673336 reads in 100.492 CPU sec, 3.224 real sec
sorting block 77, #reads = 1079425 mem = 536877972 peak = 967663616
[M::process] read 673136 sequences (100000070 bp) in 1.710 CPU sec, 1.898 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284962, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.87, 134.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 199, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2120)
[M::mem_pestat] mean and std.dev: (420.86, 502.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2818)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673024 sequences (100000102 bp) in 1.070 CPU sec, 1.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284467, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.28, 143.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 365, 669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1957)
[M::mem_pestat] mean and std.dev: (401.33, 564.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2658)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284416, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.88, 141.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 22, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 217)
[M::mem_pestat] mean and std.dev: (30.00, 21.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 282)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673446 reads in 260.622 CPU sec, 8.372 real sec
[M::mem_process_seqs] Processed 673136 reads in 182.676 CPU sec, 5.907 real sec
[M::mem_process_seqs] Processed 673266 reads in 190.396 CPU sec, 6.183 real sec
sorting block 78, #reads = 1080236 mem = 536877333 peak = 967663616
sorting block 79, #reads = 1079537 mem = 536880602 peak = 967663616
[M::process] read 672944 sequences (100000021 bp) in 1.878 CPU sec, 2.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284081, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (275.25, 145.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672880 sequences (100000056 bp) in 1.134 CPU sec, 1.226 real sec...
[M::process] read 101288 sequences (15055226 bp) in 0.300 CPU sec, 0.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283597, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (280.61, 151.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673024 reads in 187.905 CPU sec, 6.053 real sec
[M::mem_process_seqs] Processed 672944 reads in 104.032 CPU sec, 3.370 real sec
sorting block 80, #reads = 1079610 mem = 536879894 peak = 967663616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283848, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (280.34, 152.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 42638, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (281.50, 154.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672880 reads in 108.979 CPU sec, 3.550 real sec
[M::mem_process_seqs] Processed 101288 reads in 23.092 CPU sec, 0.763 real sec
sorting block 81, #reads = 1079354 mem = 536881792 peak = 977047552
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 88272130
overall: 70039457792 mem 14064.704 user 700.557 sys 832.731 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L004\tPU:L004\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.T18-D0003255_R1.fastq.gz 0003.T18-D0003255_R2.fastq.gz
[main] Real time: 832.760 sec; CPU: 14765.263 sec
sorting block 82, #reads = 378945 mem = 188719950 peak = 977047552
uniq header count 1
merging 83 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 3370896999
output reads: 88904530
bam_mem_sort: 83 calls 40.835 user 0.535 sys 41.515 real
bam_write: 83 calls 22.813 user 1.208 sys 24.202 real
execute: 1 calls 17.469 user 0.804 sys 852.776 real
merge_files: 1 calls 17.458 user 0.773 sys 19.613 real
parse_chunk: 1318 calls 51.734 user 13.566 sys 66.555 real
read_chunk: 3547 calls 31.108 user 27.271 sys 448.765 real
sort_block: 1 calls 24.337 user 26.652 sys 437.485 real
write_chunk: 2227 calls 17.104 user 0.592 sys 18.541 real
overall: 2414452736 mem 768.135 user 57.548 sys 852.795 real
ls: cannot access '*.cram': No such file or directory