+ mkdir RGB_RLP_lowDV_1ststrand_25C_2
+ all_qc -bam RGB_RLP_lowDV_1ststrand_25C_2.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder RGB_RLP_lowDV_1ststrand_25C_2 -reference ref_genome.fa -region chr20
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[mpileup] 1 samples in 1 input files
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
/opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
import pkg_resources
/opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
import pkg_resources
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
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5056116it [00:03, 1698264.59it/s][W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
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[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
[W::hts_idx_load3] The index file is older than the data file: RGB_RLP_lowDV_1ststrand_25C_2.md.bai
+ merge_qc -folders RGB_RLP_lowDV_1ststrand_25C_2/RGB_RLP_lowDV_1ststrand_25C_2.md -output RGB_RLP_lowDV_1ststrand_25C_2/RGB_RLP_lowDV_1ststrand_25C_2_full_basic_stats.tsv
+ head -n1 RGB_RLP_lowDV_1ststrand_25C_2/RGB_RLP_lowDV_1ststrand_25C_2_full_basic_stats.tsv
++ basename Twist_rna_exome_AND_fusion_hg38.bed
+ grep -e Twist_rna_exome_AND_fusion_hg38.bed RGB_RLP_lowDV_1ststrand_25C_2/RGB_RLP_lowDV_1ststrand_25C_2_full_basic_stats.tsv