[2026-06-08T15:55:07] Launching Arriba 2.4.0
[2026-06-08T15:55:07] Loading assembly from 'ref_genome.fa' 
[2026-06-08T15:55:16] Loading annotation from 'ref_annot.gtf' 
[2026-06-08T15:55:19] Reading chimeric alignments from 'rna_s1221_S42.Aligned.sortedByCoord.out.bam' (total=641586)
[2026-06-08T15:55:34] Marking multi-mapping alignments (marked=528121)
[2026-06-08T15:55:35] Detecting strandedness (reverse)
[2026-06-08T15:55:35] Assigning strands to alignments 
[2026-06-08T15:55:35] Annotating alignments 
[2026-06-08T15:55:36] Filtering duplicates (remaining=451162)
[2026-06-08T15:55:37] Filtering mates which do not map to interesting contigs (1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y AC_* NC_*) (remaining=300486)
[2026-06-08T15:55:37] Filtering mates which only map to viral contigs (AC_* NC_*) (remaining=300486)
[2026-06-08T15:55:37] Filtering viral contigs with expression lower than the top 5 (remaining=300486)
[2026-06-08T15:55:37] Filtering viral contigs with less than 5% coverage (remaining=300486)
[2026-06-08T15:55:37] Estimating fragment length (mate gap mean=-75.3836, mate gap stddev=35.9642, read length mean=142.703)
[2026-06-08T15:55:37] Filtering read-through fragments with a distance <=10000bp (remaining=287815)
[2026-06-08T15:55:38] Filtering inconsistently clipped mates (remaining=278243)
[2026-06-08T15:55:38] Filtering breakpoints adjacent to homopolymers >=6nt (remaining=265896)
[2026-06-08T15:55:38] Filtering fragments with small insert size (remaining=265860)
[2026-06-08T15:55:38] Filtering alignments with long gaps (remaining=265860)
[2026-06-08T15:55:38] Filtering fragments with both mates in the same gene (remaining=265817)
[2026-06-08T15:55:38] Filtering fusions arising from hairpin structures (remaining=242483)
[2026-06-08T15:55:38] Filtering reads with a mismatch p-value <=0.01 (remaining=161410)
[2026-06-08T15:55:39] Filtering reads with low entropy (k-mer content >=60%) (remaining=98982)
[2026-06-08T15:55:39] Finding fusions and counting supporting reads (total=78969)
[2026-06-08T15:55:40] Merging adjacent fusion breakpoints (remaining=78844)
[2026-06-08T15:55:40] Filtering multi-mapping fusions by alignment score and read support (remaining=32035)
[2026-06-08T15:55:41] Estimating expected number of fusions by random chance (e-value) 
[2026-06-08T15:55:41] Filtering fusions with both breakpoints in adjacent non-coding/intergenic regions (remaining=31724)
[2026-06-08T15:55:41] Filtering intragenic fusions with both breakpoints in exonic regions (remaining=25969)
[2026-06-08T15:55:41] Filtering fusions with <2 supporting reads (remaining=2505)
[2026-06-08T15:55:41] Filtering fusions with an e-value >=0.3 (remaining=1067)
[2026-06-08T15:55:41] Searching for internal tandem duplications <=100bp with >=10 supporting reads and >=7% allele fraction (remaining=1083)
[2026-06-08T15:55:42] Filtering fusions with both breakpoints in intronic/intergenic regions (remaining=1070)
[2026-06-08T15:55:42] Searching for known fusions in 'known_fusions_hg38_GRCh38_v2.4.0.tsv.gz' (remaining=1071)
[2026-06-08T15:55:42] Filtering in vitro-generated fusions between genes with an expression above the 99.8% quantile (remaining=954)
[2026-06-08T15:55:42] Searching for fusions with spliced split reads (remaining=965)
[2026-06-08T15:55:42] Selecting best breakpoints from genes with multiple breakpoints (remaining=569)
[2026-06-08T15:55:42] Filtering read-through fusions with breakpoints near the gene boundary (remaining=566)
[2026-06-08T15:55:42] Searching for fusions with >=4 spliced events (remaining=570)
[2026-06-08T15:55:42] Filtering blacklisted fusions in 'blacklist_hg38_GRCh38_v2.4.0.tsv.gz' (remaining=128)
[2026-06-08T15:55:51] Filtering fusions with anchors <=23nt (remaining=81)
[2026-06-08T15:55:52] Filtering end-to-end fusions with low support (remaining=76)
[2026-06-08T15:55:52] Filtering fusions with no coverage around the breakpoints (remaining=72)
[2026-06-08T15:55:52] Indexing gene sequences 
[2026-06-08T15:55:52] Filtering genes with >=30% identity (remaining=31)
[2026-06-08T15:55:52] Re-aligning chimeric reads to filter fusions with >=80% mis-mappers (remaining=24)
[2026-06-08T15:55:52] Selecting best breakpoints from genes with multiple breakpoints (remaining=24)
[2026-06-08T15:55:52] Searching for additional isoforms (remaining=25)
[2026-06-08T15:55:52] Assigning confidence scores to events 
[2026-06-08T15:55:52] Loading protein domains from 'protein_domains_hg38_GRCh38_v2.4.0.gff3'
[2026-06-08T15:55:53] Writing fusions to file 'rna_s1221_S42.arriba.fusions.tsv' 
[2026-06-08T15:55:53] Writing discarded fusions to file 'rna_s1221_S42.arriba.fusions.discarded.tsv'
[2026-06-08T15:55:54] Freeing resources
[2026-06-08T15:55:55] Done (elapsed time=00:00:48, CPU time=00:00:48, peak memory=4.8gb)