/usr/local/bin/picard: line 5: warning: setlocale: LC_ALL: cannot change locale (en_US.UTF-8): No such file or directory INFO 2026-05-29 17:30:37 RExecutor Executing R script via command: Rscript /tmp/script8075757307372410128.R ERROR 2026-05-29 17:30:37 ProcessExecutor /bin/sh: warning: setlocale: LC_ALL: cannot change locale (en_US.UTF-8) ERROR 2026-05-29 17:30:37 ProcessExecutor sh: warning: setlocale: LC_ALL: cannot change locale (en_US.UTF-8) ERROR 2026-05-29 17:30:37 ProcessExecutor sh: warning: setlocale: LC_ALL: cannot change locale (en_US.UTF-8) ERROR 2026-05-29 17:30:37 ProcessExecutor During startup - Warning messages: ERROR 2026-05-29 17:30:37 ProcessExecutor 1: Setting LC_CTYPE failed, using "C" ERROR 2026-05-29 17:30:37 ProcessExecutor 2: Setting LC_COLLATE failed, using "C" ERROR 2026-05-29 17:30:37 ProcessExecutor 3: Setting LC_TIME failed, using "C" ERROR 2026-05-29 17:30:37 ProcessExecutor 4: Setting LC_MESSAGES failed, using "C" ERROR 2026-05-29 17:30:37 ProcessExecutor 5: Setting LC_MONETARY failed, using "C" ERROR 2026-05-29 17:30:37 ProcessExecutor 6: Setting LC_PAPER failed, using "C" ERROR 2026-05-29 17:30:37 ProcessExecutor 7: Setting LC_MEASUREMENT failed, using "C" INFO 2026-05-29 17:30:37 ProcessExecutor [1] "Checking if R is installed" ERROR 2026-05-29 17:30:37 ProcessExecutor sh: warning: setlocale: LC_ALL: cannot change locale (en_US.UTF-8) May 29, 2026 5:30:37 PM com.intel.gkl.NativeLibraryLoader load INFO: Loading libgkl_compression.so from jar:file:/usr/local/share/picard-3.1.0-0/picard.jar!/com/intel/gkl/native/libgkl_compression.so [Fri May 29 17:30:37 GMT 2026] CollectInsertSizeMetrics --Histogram_FILE rna_s1221_S42_collectinsertsize.pdf --INPUT rna_s1221_S42.Aligned.sortedByCoord.out.bam --OUTPUT rna_s1221_S42_collectinsertsize.txt --DEVIATIONS 10.0 --MINIMUM_PCT 0.05 --METRIC_ACCUMULATION_LEVEL ALL_READS --INCLUDE_DUPLICATES false --ASSUME_SORTED true --STOP_AFTER 0 --VERBOSITY INFO --QUIET false --VALIDATION_STRINGENCY STRICT --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false [Fri May 29 17:30:37 GMT 2026] Executing as ?@nf-b74130d7c1024fa117ca311668103ed6-e4aac-wbrns on Linux 6.12.83 amd64; OpenJDK 64-Bit Server VM 20-internal-adhoc..src; Deflater: Intel; Inflater: Intel; Provider GCS is available; Picard version: Version:3.1.0 INFO 2026-05-29 17:30:39 SinglePassSamProgram Processed 1,000,000 records. Elapsed time: 00:00:01s. Time for last 1,000,000: 1s. Last read position: chr1:110,018,371 INFO 2026-05-29 17:30:41 SinglePassSamProgram Processed 2,000,000 records. Elapsed time: 00:00:03s. Time for last 1,000,000: 1s. Last read position: chr1:228,146,888 INFO 2026-05-29 17:30:42 SinglePassSamProgram Processed 3,000,000 records. Elapsed time: 00:00:04s. Time for last 1,000,000: 1s. Last read position: chr2:159,759,241 INFO 2026-05-29 17:30:43 SinglePassSamProgram Processed 4,000,000 records. Elapsed time: 00:00:05s. Time for last 1,000,000: 1s. Last read position: chr3:51,993,725 INFO 2026-05-29 17:30:44 SinglePassSamProgram Processed 5,000,000 records. Elapsed time: 00:00:06s. Time for last 1,000,000: 1s. Last read position: chr4:102,597,542 INFO 2026-05-29 17:30:46 SinglePassSamProgram Processed 6,000,000 records. Elapsed time: 00:00:08s. Time for last 1,000,000: 1s. Last read position: chr5:176,506,768 INFO 2026-05-29 17:30:47 SinglePassSamProgram Processed 7,000,000 records. Elapsed time: 00:00:09s. Time for last 1,000,000: 1s. Last read position: chr6:34,421,771 INFO 2026-05-29 17:30:48 SinglePassSamProgram Processed 8,000,000 records. Elapsed time: 00:00:10s. Time for last 1,000,000: 1s. Last read position: chr7:26,197,277 INFO 2026-05-29 17:30:49 SinglePassSamProgram Processed 9,000,000 records. Elapsed time: 00:00:11s. Time for last 1,000,000: 1s. Last read position: chr8:89,914,196 INFO 2026-05-29 17:30:50 SinglePassSamProgram Processed 10,000,000 records. Elapsed time: 00:00:12s. Time for last 1,000,000: 1s. Last read position: chr9:133,349,561 INFO 2026-05-29 17:30:51 SinglePassSamProgram Processed 11,000,000 records. Elapsed time: 00:00:13s. Time for last 1,000,000: 1s. Last read position: chr11:33,068,720 INFO 2026-05-29 17:30:52 SinglePassSamProgram Processed 12,000,000 records. Elapsed time: 00:00:14s. Time for last 1,000,000: 1s. Last read position: chr12:6,945,786 INFO 2026-05-29 17:30:53 SinglePassSamProgram Processed 13,000,000 records. Elapsed time: 00:00:15s. Time for last 1,000,000: 1s. Last read position: chr12:122,752,900 INFO 2026-05-29 17:30:54 SinglePassSamProgram Processed 14,000,000 records. Elapsed time: 00:00:16s. Time for last 1,000,000: 1s. Last read position: chr15:28,076,562 INFO 2026-05-29 17:30:56 SinglePassSamProgram Processed 15,000,000 records. Elapsed time: 00:00:18s. Time for last 1,000,000: 1s. Last read position: chr16:21,834,735 INFO 2026-05-29 17:30:57 SinglePassSamProgram Processed 16,000,000 records. Elapsed time: 00:00:19s. Time for last 1,000,000: 1s. Last read position: chr17:7,318,111 INFO 2026-05-29 17:30:58 SinglePassSamProgram Processed 17,000,000 records. Elapsed time: 00:00:20s. Time for last 1,000,000: 1s. Last read position: chr17:76,039,898 INFO 2026-05-29 17:30:59 SinglePassSamProgram Processed 18,000,000 records. Elapsed time: 00:00:21s. Time for last 1,000,000: 1s. Last read position: chr19:12,734,557 INFO 2026-05-29 17:31:00 SinglePassSamProgram Processed 19,000,000 records. Elapsed time: 00:00:22s. Time for last 1,000,000: 1s. Last read position: chr20:35,277,516 INFO 2026-05-29 17:31:01 SinglePassSamProgram Processed 20,000,000 records. Elapsed time: 00:00:23s. Time for last 1,000,000: 1s. Last read position: chr21:8,398,054 INFO 2026-05-29 17:31:02 SinglePassSamProgram Processed 21,000,000 records. Elapsed time: 00:00:24s. Time for last 1,000,000: 1s. Last read position: chr22:38,495,046 INFO 2026-05-29 17:31:03 SinglePassSamProgram Processed 22,000,000 records. Elapsed time: 00:00:25s. Time for last 1,000,000: 1s. Last read position: chrY:19,721,298 INFO 2026-05-29 17:31:04 SinglePassSamProgram Processed 23,000,000 records. Elapsed time: 00:00:26s. Time for last 1,000,000: 0s. Last read position: KI270733.1:134,152 INFO 2026-05-29 17:31:04 RExecutor Executing R script via command: Rscript /tmp/script13311346842682533400.R /tmp/nxf.XXXXbAXGqN/rna_s1221_S42_collectinsertsize.txt /tmp/nxf.XXXXbAXGqN/rna_s1221_S42_collectinsertsize.pdf rna_s1221_S42.Aligned.sortedByCoord.out.bam ERROR 2026-05-29 17:31:04 ProcessExecutor /bin/sh: warning: setlocale: LC_ALL: cannot change locale (en_US.UTF-8) ERROR 2026-05-29 17:31:04 ProcessExecutor sh: warning: setlocale: LC_ALL: cannot change locale (en_US.UTF-8) ERROR 2026-05-29 17:31:04 ProcessExecutor sh: warning: setlocale: LC_ALL: cannot change locale (en_US.UTF-8) ERROR 2026-05-29 17:31:05 ProcessExecutor During startup - Warning messages: ERROR 2026-05-29 17:31:05 ProcessExecutor 1: Setting LC_CTYPE failed, using "C" ERROR 2026-05-29 17:31:05 ProcessExecutor 2: Setting LC_COLLATE failed, using "C" ERROR 2026-05-29 17:31:05 ProcessExecutor 3: Setting LC_TIME failed, using "C" ERROR 2026-05-29 17:31:05 ProcessExecutor 4: Setting LC_MESSAGES failed, using "C" ERROR 2026-05-29 17:31:05 ProcessExecutor 5: Setting LC_MONETARY failed, using "C" ERROR 2026-05-29 17:31:05 ProcessExecutor 6: Setting LC_PAPER failed, using "C" ERROR 2026-05-29 17:31:05 ProcessExecutor 7: Setting LC_MEASUREMENT failed, using "C" INFO 2026-05-29 17:31:05 ProcessExecutor null device INFO 2026-05-29 17:31:05 ProcessExecutor 1 ERROR 2026-05-29 17:31:05 ProcessExecutor sh: warning: setlocale: LC_ALL: cannot change locale (en_US.UTF-8) [Fri May 29 17:31:05 GMT 2026] picard.analysis.CollectInsertSizeMetrics done. Elapsed time: 0.45 minutes. Runtime.totalMemory()=285212672