Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/38/7e00883c5855145cdfdb184e7505f2/rna_s1221_S42.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/58/1e2fcf1754af77a22a09e2e77c303e/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9c/4a6738b72653a8444bbe026bec4235/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9c/4a6738b72653a8444bbe026bec4235/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/85/6ed28fc3a27c2ed6889fd01426200d/rna_s1221_S42.Aligned.sortedByCoord.out.bam
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: rna_s1221_S42.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (560912) splicing reads. 93862 splicing junctions. 89521 known splicing junctions. 4341 novel splicing junctions.
sampling 10% (1121824) splicing reads. 112605 splicing junctions. 104672 known splicing junctions. 7933 novel splicing junctions.
sampling 15% (1682736) splicing reads. 123534 splicing junctions. 112368 known splicing junctions. 11166 novel splicing junctions.
sampling 20% (2243649) splicing reads. 131339 splicing junctions. 117376 known splicing junctions. 13963 novel splicing junctions.
sampling 25% (2804561) splicing reads. 137490 splicing junctions. 120985 known splicing junctions. 16505 novel splicing junctions.
sampling 30% (3365473) splicing reads. 142627 splicing junctions. 123849 known splicing junctions. 18778 novel splicing junctions.
sampling 35% (3926385) splicing reads. 147036 splicing junctions. 126155 known splicing junctions. 20881 novel splicing junctions.
sampling 40% (4487298) splicing reads. 150893 splicing junctions. 128091 known splicing junctions. 22802 novel splicing junctions.
sampling 45% (5048210) splicing reads. 154345 splicing junctions. 129746 known splicing junctions. 24599 novel splicing junctions.
sampling 50% (5609122) splicing reads. 157413 splicing junctions. 131193 known splicing junctions. 26220 novel splicing junctions.
sampling 55% (6170034) splicing reads. 160108 splicing junctions. 132456 known splicing junctions. 27652 novel splicing junctions.
sampling 60% (6730947) splicing reads. 162596 splicing junctions. 133550 known splicing junctions. 29046 novel splicing junctions.
sampling 65% (7291859) splicing reads. 164918 splicing junctions. 134533 known splicing junctions. 30385 novel splicing junctions.
sampling 70% (7852771) splicing reads. 167042 splicing junctions. 135393 known splicing junctions. 31649 novel splicing junctions.
sampling 75% (8413683) splicing reads. 168972 splicing junctions. 136212 known splicing junctions. 32760 novel splicing junctions.
sampling 80% (8974596) splicing reads. 170761 splicing junctions. 136916 known splicing junctions. 33845 novel splicing junctions.
sampling 85% (9535508) splicing reads. 172337 splicing junctions. 137509 known splicing junctions. 34828 novel splicing junctions.
sampling 90% (10096420) splicing reads. 173782 splicing junctions. 138081 known splicing junctions. 35701 novel splicing junctions.
sampling 95% (10657332) splicing reads. 175117 splicing junctions. 138606 known splicing junctions. 36511 novel splicing junctions.
sampling 100% (11218245) splicing reads. 176197 splicing junctions. 139062 known splicing junctions. 37135 novel splicing junctions.
null device 
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