Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/51/a0f89b39dab3e31a4376f94755f168/rna_s1221_S42.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f8/ba9b920cffe23f04e8893f19642591/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5d/19107ebe17d2dc434ec7dbcba17d6e/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5d/19107ebe17d2dc434ec7dbcba17d6e/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/17/3c15023005fc6ecaab130723455b3f/rna_s1221_S42.Aligned.sortedByCoord.out.bam
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: rna_s1221_S42.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (560912) splicing reads. 94180 splicing junctions. 89740 known splicing junctions. 4440 novel splicing junctions.
sampling 10% (1121824) splicing reads. 112992 splicing junctions. 104949 known splicing junctions. 8043 novel splicing junctions.
sampling 15% (1682736) splicing reads. 123667 splicing junctions. 112406 known splicing junctions. 11261 novel splicing junctions.
sampling 20% (2243649) splicing reads. 131342 splicing junctions. 117296 known splicing junctions. 14046 novel splicing junctions.
sampling 25% (2804561) splicing reads. 137486 splicing junctions. 120951 known splicing junctions. 16535 novel splicing junctions.
sampling 30% (3365473) splicing reads. 142601 splicing junctions. 123782 known splicing junctions. 18819 novel splicing junctions.
sampling 35% (3926385) splicing reads. 147045 splicing junctions. 126087 known splicing junctions. 20958 novel splicing junctions.
sampling 40% (4487298) splicing reads. 151000 splicing junctions. 128044 known splicing junctions. 22956 novel splicing junctions.
sampling 45% (5048210) splicing reads. 154495 splicing junctions. 129765 known splicing junctions. 24730 novel splicing junctions.
sampling 50% (5609122) splicing reads. 157537 splicing junctions. 131184 known splicing junctions. 26353 novel splicing junctions.
sampling 55% (6170034) splicing reads. 160339 splicing junctions. 132456 known splicing junctions. 27883 novel splicing junctions.
sampling 60% (6730947) splicing reads. 162768 splicing junctions. 133524 known splicing junctions. 29244 novel splicing junctions.
sampling 65% (7291859) splicing reads. 165094 splicing junctions. 134544 known splicing junctions. 30550 novel splicing junctions.
sampling 70% (7852771) splicing reads. 167176 splicing junctions. 135415 known splicing junctions. 31761 novel splicing junctions.
sampling 75% (8413683) splicing reads. 169099 splicing junctions. 136182 known splicing junctions. 32917 novel splicing junctions.
sampling 80% (8974596) splicing reads. 170773 splicing junctions. 136911 known splicing junctions. 33862 novel splicing junctions.
sampling 85% (9535508) splicing reads. 172333 splicing junctions. 137531 known splicing junctions. 34802 novel splicing junctions.
sampling 90% (10096420) splicing reads. 173727 splicing junctions. 138090 known splicing junctions. 35637 novel splicing junctions.
sampling 95% (10657332) splicing reads. 175019 splicing junctions. 138621 known splicing junctions. 36398 novel splicing junctions.
sampling 100% (11218245) splicing reads. 176197 splicing junctions. 139062 known splicing junctions. 37135 novel splicing junctions.
null device 
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