Done.
-parsing GTF file: /tmp/nxf.sjoUE5mknD/rna_s1221_S42.gtf
* Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.sjoUE5mknD/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.sjoUE5mknD/rna_s1221_S42_1.fastp.fastq.gz /tmp/nxf.sjoUE5mknD/rna_s1221_S42_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.sjoUE5mknD/fi_workdir/rna_s1221_S42.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.sjoUE5mknD/fi_workdir/rna_s1221_S42.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c'
* Running CMD: mv Aligned.sortedByCoord.out.bam rna_s1221_S42.star.sortedByCoord.out.bam
* Running CMD: samtools index rna_s1221_S42.star.sortedByCoord.out.bam
13:21:17 : INFO : Done.
-parsing GTF file: /tmp/nxf.sjoUE5mknD/fi_workdir/rna_s1221_S42.gtf
-parsing /tmp/nxf.sjoUE5mknD/fi_workdir/rna_s1221_S42.star.cSorted.dupsMarked.bam
[10000]
[20000]
[30000]
[40000]
[50000]
[60000] -done parsing /tmp/nxf.sjoUE5mknD/fi_workdir/rna_s1221_S42.star.cSorted.dupsMarked.bam. Extracting junction info.
junction read elimination tally: $VAR1 = {
'excessive soft clipping' => 626,
'num genes matched < 2' => 3,
'per_id < 96' => 179,
' ** passed ** ' => 440,
'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 3,
'low complexity anchor region' => 2
};
-writing fusion junction support info.
-outputting the spanning read info: /tmp/nxf.sjoUE5mknD/fi_workdir/rna_s1221_S42.star.cSorted.dupsMarked.bam.fusion_spanning_info.
- counting read alignments among fusion contigs.
[1000] -fusion SPANNING read extraction for scaff: AC015911.9--SLFN12L
[1000] -fusion SPANNING read extraction for scaff: CCDC6--RET
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000]
[18000]
[19000]
[20000]
[21000]
[22000]
[23000]
[24000]
[25000]
[26000]
[27000]
[28000]
[29000] -fusion SPANNING read extraction for scaff: CD74--ROS1
-fusion SPANNING read extraction for scaff: EGFR--SEPTIN14
[1000]
[2000] -fusion SPANNING read extraction for scaff: ETV6--NTRK3
[1000]
[2000]
[3000]
[4000] -fusion SPANNING read extraction for scaff: FGFR3--TACC3
-fusion SPANNING read extraction for scaff: FGFR3--BAIAP2L1
[1000]
[2000]
[3000] -fusion SPANNING read extraction for scaff: KIF5B--RET
[1000]
[2000]
[3000]
[4000] -fusion SPANNING read extraction for scaff: LMNA--NTRK1
[1000]
[2000]
[3000] -fusion SPANNING read extraction for scaff: NCOA4--RET
-fusion SPANNING read extraction for scaff: PAX8--PPARG
[1000]
[2000] -fusion SPANNING read extraction for scaff: SLC45A3--BRAF
[1000]
[2000] -fusion SPANNING read extraction for scaff: TFG--NTRK1
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000] -fusion SPANNING read extraction for scaff: TPM3--NTRK1
-filtered reads reasons: $VAR1 = {
'seq similar region alignment' => 546,
'lacks exon overlap' => 306
};
EM: Starting log likelihood: -693.427028
EM: Round [1] log likelihood: -693.427028
EM: Stopping iterations at round 1 due to insufficient improvement in likelihood.
-extracting read coordinates from /tmp/nxf.sjoUE5mknD/rna_s1221_S42.consolidated.bam into /tmp/nxf.sjoUE5mknD/rna_s1221_S42.consolidated.bam.read_coords
CMD: touch /tmp/nxf.sjoUE5mknD/rna_s1221_S42.consolidated.bam.read_coords.ok
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.sjoUE5mknD/rna_s1221_S42.consolidated.bam.read_coords > /tmp/nxf.sjoUE5mknD/rna_s1221_S42.consolidated.bam.read_coords.sort_by_readname
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.sjoUE5mknD/rna_s1221_S42.consolidated.bam.frag_coords > /tmp/nxf.sjoUE5mknD/rna_s1221_S42.consolidated.bam.frag_coords.coord_sorted
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.sjoUE5mknD/fi_workdir/rna_s1221_S42.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.sjoUE5mknD/fi_workdir/rna_s1221_S42.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50
CMD: cp /tmp/nxf.sjoUE5mknD/fi_workdir/rna_s1221_S42.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.sjoUE5mknD/rna_s1221_S42.post_blast_and_promiscuity_filter
-done, see /tmp/nxf.sjoUE5mknD/rna_s1221_S42.post_blast_and_promiscuity_filter.annot_filter.pass
EM: Starting log likelihood: -673.637338
EM: Round [1] log likelihood: -673.637338
EM: Stopping iterations at round 1 due to insufficient improvement in likelihood.
-total frags in /tmp/nxf.sjoUE5mknD/rna_s1221_S42_1.fastp.fastq.gz: 9928008
Warning - not locating file: /tmp/nxf.sjoUE5mknD/rna_s1221_S42.gmap_trinity_GG.fusions.gff3.bed
WARNING, no entry stored in dbm for [ENSG00000286030.2]
ls: cannot access 'IGV_inputs': No such file or directory