Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2e/ff571f0e60eccfcbe60f1470f0b807/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/daq/20260511_LH00527_0083_A23TNVHLT4__8afaba2b--20260513-165037/alignments/recalibrated/1136_8DR-T1-TDNA-1_A23TNVHLT4_1/1136_8DR-T1-TDNA-1_A23TNVHLT4_1.recalibrated.bam Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/daq/20260511_LH00527_0083_A23TNVHLT4__8afaba2b--20260513-165037/alignments/recalibrated/1136_8DR-T1-TDNA-1_A23TNVHLT4_1/1136_8DR-T1-TDNA-1_A23TNVHLT4_1.recalibrated.bam.bai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ec/b6bfd04cf8e0046cc54e98c4afebf9/1136_8DR-T1-TDNA-1_A23TNVHLT4_1_vs_1136_8DR-N1-BDNA-1_A23NLN3LT4_1.snv_indel.merged.vcf.gz.tbi Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ec/b6bfd04cf8e0046cc54e98c4afebf9/1136_8DR-T1-TDNA-1_A23TNVHLT4_1_vs_1136_8DR-N1-BDNA-1_A23NLN3LT4_1.snv_indel.merged.vcf.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2e/ff571f0e60eccfcbe60f1470f0b807/.command.run ==> STAGING COMPLETE (8 inputs) [W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_A23TNVHLT4_1_vs_1136_8DR-N1-BDNA-1_A23NLN3LT4_1.snv_indel.merged.vcf.gz.tbi This is WhatsHap 2.8 running under Python 3.9.23 [W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_A23TNVHLT4_1.recalibrated.bam.bai [W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_A23TNVHLT4_1.recalibrated.bam.bai [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' Working on 2 samples from 2 families WARNING: Skipping duplicated position 1067011 on chromosome 'chr17' Hiding further warnings of this type, use --debug to show # Working on contig chr17 in individual 1136_8DR-N1-BDNA-1_A23NLN3LT4_1 Found 43 usable heterozygous variants (0 skipped due to missing genotypes) WARNING: Sample '1136_8DR-N1-BDNA-1_A23NLN3LT4_1' not found in any BAM/CRAM file. Found 0 reads covering 0 variants Kept 0 reads that cover at least two variants each Selected 0 most phase-informative reads covering 0 variants Best-case phasing would result in 0 non-singleton phased blocks (0 singletons). Phasing 1 sample by solving the MEC problem ... No. of phased blocks: 0 # Working on contig chr17 in individual 1136_8DR-T1-TDNA-1_A23TNVHLT4_1 Found 709 usable heterozygous variants (0 skipped due to missing genotypes) [W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_A23TNVHLT4_1.recalibrated.bam.bai Number of supplementary alignments: 0 Number of non-singleton groups: 34275 Skipped 0 groups Found 129881 reads covering 709 variants Kept 20240 reads that cover at least two variants each Selected 1515 most phase-informative reads covering 266 variants Best-case phasing would result in 101 non-singleton phased blocks (0 singletons). Phasing 1 sample by solving the MEC problem ... Largest block contains 16 variants (6.0% of accessible variants) between position 41084252 and 41140109 WARNING: Ignoring existing phasing information found in input VCF (PS tag exists). # Resource usage Maximum memory usage: 0.237 GB Time spent reading BAM/CRAM: 147.6 s Time spent parsing VCF: 0.0 s Time spent selecting reads: 0.7 s Time spent phasing: 0.7 s Time spent writing VCF: 0.0 s Time spent finding components: 0.0 s Time spent on rest: 0.2 s Total elapsed time: 149.2 s