Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/00/8d68e29e565aa382be4b16a56df782/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9f/262353cbd4618d6171bdb789c05fd9/1136_8DR-T1-TDNA-1_B23TTLKLT4_1_vs_1136_8DR-N1-BDNA-1_A23NLN3LT4_1.snv_indel.merged.vcf.gz Downloading: s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/daq/20260511_LH00527_0084_B23TTLKLT4__8afaba2b--20260513-165040/alignments/recalibrated/1136_8DR-T1-TDNA-1_B23TTLKLT4_1/1136_8DR-T1-TDNA-1_B23TTLKLT4_1.recalibrated.bam.bai Downloading: s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/daq/20260511_LH00527_0084_B23TTLKLT4__8afaba2b--20260513-165040/alignments/recalibrated/1136_8DR-T1-TDNA-1_B23TTLKLT4_1/1136_8DR-T1-TDNA-1_B23TTLKLT4_1.recalibrated.bam Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/00/8d68e29e565aa382be4b16a56df782/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9f/262353cbd4618d6171bdb789c05fd9/1136_8DR-T1-TDNA-1_B23TTLKLT4_1_vs_1136_8DR-N1-BDNA-1_A23NLN3LT4_1.snv_indel.merged.vcf.gz.tbi ==> STAGING COMPLETE (8 inputs) This is WhatsHap 2.8 running under Python 3.9.23 [W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_B23TTLKLT4_1.recalibrated.bam.bai [W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_B23TTLKLT4_1.recalibrated.bam.bai [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' Working on 2 samples from 2 families WARNING: Skipping duplicated position 15640980 on chromosome 'chr4' Hiding further warnings of this type, use --debug to show # Working on contig chr4 in individual 1136_8DR-N1-BDNA-1_A23NLN3LT4_1 Found 46 usable heterozygous variants (0 skipped due to missing genotypes) WARNING: Sample '1136_8DR-N1-BDNA-1_A23NLN3LT4_1' not found in any BAM/CRAM file. Found 0 reads covering 0 variants Kept 0 reads that cover at least two variants each Selected 0 most phase-informative reads covering 0 variants Best-case phasing would result in 0 non-singleton phased blocks (0 singletons). Phasing 1 sample by solving the MEC problem ... No. of phased blocks: 0 # Working on contig chr4 in individual 1136_8DR-T1-TDNA-1_B23TTLKLT4_1 Found 606 usable heterozygous variants (0 skipped due to missing genotypes) [W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_B23TTLKLT4_1.recalibrated.bam.bai Number of supplementary alignments: 0 Number of non-singleton groups: 39077 Skipped 0 groups Found 144824 reads covering 606 variants Kept 22811 reads that cover at least two variants each Selected 1035 most phase-informative reads covering 183 variants Best-case phasing would result in 70 non-singleton phased blocks (0 singletons). Phasing 1 sample by solving the MEC problem ... Largest block contains 23 variants (12.6% of accessible variants) between position 87615210 and 87616316 WARNING: Ignoring existing phasing information found in input VCF (PS tag exists). # Resource usage Maximum memory usage: 0.456 GB Time spent reading BAM/CRAM: 238.4 s Time spent parsing VCF: 0.0 s Time spent selecting reads: 1.6 s Time spent phasing: 0.8 s Time spent writing VCF: 0.0 s Time spent finding components: 0.0 s Time spent on rest: 0.4 s Total elapsed time: 241.3 s