Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7d/08fe888d8884144d097192974cfdd9/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9f/262353cbd4618d6171bdb789c05fd9/1136_8DR-T1-TDNA-1_B23TTLKLT4_1_vs_1136_8DR-N1-BDNA-1_A23NLN3LT4_1.snv_indel.merged.vcf.gz Downloading: s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/daq/20260511_LH00527_0084_B23TTLKLT4__8afaba2b--20260513-165040/alignments/recalibrated/1136_8DR-T1-TDNA-1_B23TTLKLT4_1/1136_8DR-T1-TDNA-1_B23TTLKLT4_1.recalibrated.bam.bai Downloading: s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/daq/20260511_LH00527_0084_B23TTLKLT4__8afaba2b--20260513-165040/alignments/recalibrated/1136_8DR-T1-TDNA-1_B23TTLKLT4_1/1136_8DR-T1-TDNA-1_B23TTLKLT4_1.recalibrated.bam Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7d/08fe888d8884144d097192974cfdd9/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9f/262353cbd4618d6171bdb789c05fd9/1136_8DR-T1-TDNA-1_B23TTLKLT4_1_vs_1136_8DR-N1-BDNA-1_A23NLN3LT4_1.snv_indel.merged.vcf.gz.tbi ==> STAGING COMPLETE (8 inputs) This is WhatsHap 2.8 running under Python 3.9.23 [W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_B23TTLKLT4_1.recalibrated.bam.bai [W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_B23TTLKLT4_1.recalibrated.bam.bai [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' Working on 2 samples from 2 families WARNING: Skipping duplicated position 7495459 on chromosome 'chr12' Hiding further warnings of this type, use --debug to show # Working on contig chr12 in individual 1136_8DR-N1-BDNA-1_A23NLN3LT4_1 Found 0 usable heterozygous variants (0 skipped due to missing genotypes) WARNING: Sample '1136_8DR-N1-BDNA-1_A23NLN3LT4_1' not found in any BAM/CRAM file. Found 0 reads covering 0 variants Kept 0 reads that cover at least two variants each Selected 0 most phase-informative reads covering 0 variants Best-case phasing would result in 0 non-singleton phased blocks (0 singletons). Phasing 1 sample by solving the MEC problem ... No. of phased blocks: 0 # Working on contig chr12 in individual 1136_8DR-T1-TDNA-1_B23TTLKLT4_1 Found 1111 usable heterozygous variants (0 skipped due to missing genotypes) [W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_B23TTLKLT4_1.recalibrated.bam.bai Number of supplementary alignments: 0 Number of non-singleton groups: 87579 Skipped 0 groups Found 310726 reads covering 1111 variants Kept 74168 reads that cover at least two variants each Selected 2104 most phase-informative reads covering 581 variants Best-case phasing would result in 127 non-singleton phased blocks (0 singletons). Phasing 1 sample by solving the MEC problem ... Largest block contains 105 variants (18.1% of accessible variants) between position 11030508 and 11134043 WARNING: Ignoring existing phasing information found in input VCF (PS tag exists). # Resource usage Maximum memory usage: 0.549 GB Time spent reading BAM/CRAM: 659.8 s Time spent parsing VCF: 0.1 s Time spent selecting reads: 10.2 s Time spent phasing: 4.6 s Time spent writing VCF: 0.1 s Time spent finding components: 0.0 s Time spent on rest: 1.4 s Total elapsed time: 676.2 s