File Info

Filename
.command.err
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a2/3c0e565192d6454b2309586d5e8d37/.command.err
Size
2.5 KB
Attempt
This is WhatsHap 2.8 running under Python 3.9.23
[W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_B23TTLKLT4_1.recalibrated.bam.bai
[W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_B23TTLKLT4_1.recalibrated.bam.bai
[E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz'
[E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz'
[E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz'
Working on 2 samples from 2 families
WARNING: Skipping duplicated position 6640069 on chromosome 'chr1' Hiding further warnings of this type, use --debug to show

# Working on contig chr1 in individual 1136_8DR-N1-BDNA-1_A23NLN3LT4_1
Found 25 usable heterozygous variants (0 skipped due to missing genotypes)
WARNING: Sample '1136_8DR-N1-BDNA-1_A23NLN3LT4_1' not found in any BAM/CRAM file.
Found 0 reads covering 0 variants
Kept 0 reads that cover at least two variants each
Selected 0 most phase-informative reads covering 0 variants
Best-case phasing would result in 0 non-singleton phased blocks (0 singletons). 
Phasing 1 sample by solving the MEC problem ...
No. of phased blocks: 0

# Working on contig chr1 in individual 1136_8DR-T1-TDNA-1_B23TTLKLT4_1
Found 1771 usable heterozygous variants (0 skipped due to missing genotypes)
[W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_B23TTLKLT4_1.recalibrated.bam.bai
Number of supplementary alignments: 0
Number of non-singleton groups: 159033
Skipped 0 groups
Found 548676 reads covering 1771 variants
Kept 118445 reads that cover at least two variants each
Selected 3738 most phase-informative reads covering 796 variants
Best-case phasing would result in 236 non-singleton phased blocks (0 singletons). 
Phasing 1 sample by solving the MEC problem ...
Largest block contains 52 variants (6.5% of accessible variants) between position 152213286 and 152216982
WARNING: Ignoring existing phasing information found in input VCF (PS tag exists).

# Resource usage
Maximum memory usage: 0.881 GB
Time spent reading BAM/CRAM:                  391.8 s
Time spent parsing VCF:                         0.0 s
Time spent selecting reads:                     4.5 s
Time spent phasing:                             2.1 s
Time spent writing VCF:                         0.1 s
Time spent finding components:                  0.0 s
Time spent on rest:                             0.6 s
Total elapsed time:                           399.1 s