Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/3a/e43da9db759fbfa968aa83560fb8d7/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/73/1d4ab20980f8d731eb1f1cf3684bf8/1029_01F-T1-TDNA-1_A23TNVYLT4_1_vs_1029_01F-N1-BDNA-1_B23KFG5LT4_1.snv_indel.merged.vcf.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/73/1d4ab20980f8d731eb1f1cf3684bf8/1029_01F-T1-TDNA-1_A23TNVYLT4_1_vs_1029_01F-N1-BDNA-1_B23KFG5LT4_1.snv_indel.merged.vcf.gz.tbi Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/daq/20260504_LH00527_0081_A23TNVYLT4__c86dea0e--20260506-131012/alignments/recalibrated/1029_01F-T1-TDNA-1_A23TNVYLT4_1/1029_01F-T1-TDNA-1_A23TNVYLT4_1.recalibrated.bam Downloading: s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/daq/20260504_LH00527_0081_A23TNVYLT4__c86dea0e--20260506-131012/alignments/recalibrated/1029_01F-T1-TDNA-1_A23TNVYLT4_1/1029_01F-T1-TDNA-1_A23TNVYLT4_1.recalibrated.bam.bai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/3a/e43da9db759fbfa968aa83560fb8d7/.command.run ==> STAGING COMPLETE (8 inputs) This is WhatsHap 2.8 running under Python 3.9.23 [W::hts_idx_load3] The index file is older than the data file: 1029_01F-T1-TDNA-1_A23TNVYLT4_1.recalibrated.bam.bai [W::hts_idx_load3] The index file is older than the data file: 1029_01F-T1-TDNA-1_A23TNVYLT4_1.recalibrated.bam.bai [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' Working on 2 samples from 2 families WARNING: Skipping duplicated position 10414738 on chromosome 'chr21' Hiding further warnings of this type, use --debug to show # Working on contig chr21 in individual 1029_01F-N1-BDNA-1_B23KFG5LT4_1 Found 0 usable heterozygous variants (0 skipped due to missing genotypes) WARNING: Sample '1029_01F-N1-BDNA-1_B23KFG5LT4_1' not found in any BAM/CRAM file. Found 0 reads covering 0 variants Kept 0 reads that cover at least two variants each Selected 0 most phase-informative reads covering 0 variants Best-case phasing would result in 0 non-singleton phased blocks (0 singletons). Phasing 1 sample by solving the MEC problem ... No. of phased blocks: 0 # Working on contig chr21 in individual 1029_01F-T1-TDNA-1_A23TNVYLT4_1 Found 234 usable heterozygous variants (0 skipped due to missing genotypes) [W::hts_idx_load3] The index file is older than the data file: 1029_01F-T1-TDNA-1_A23TNVYLT4_1.recalibrated.bam.bai Number of supplementary alignments: 0 Number of non-singleton groups: 16705 Skipped 0 groups Found 67455 reads covering 234 variants Kept 16891 reads that cover at least two variants each Selected 548 most phase-informative reads covering 124 variants Best-case phasing would result in 33 non-singleton phased blocks (0 singletons). Phasing 1 sample by solving the MEC problem ... Largest block contains 27 variants (21.8% of accessible variants) between position 44573789 and 44601223 WARNING: Ignoring existing phasing information found in input VCF (PS tag exists). # Resource usage Maximum memory usage: 0.177 GB Time spent reading BAM/CRAM: 38.3 s Time spent parsing VCF: 0.0 s Time spent selecting reads: 0.5 s Time spent phasing: 0.3 s Time spent writing VCF: 0.0 s Time spent finding components: 0.0 s Time spent on rest: 0.2 s Total elapsed time: 39.3 s