Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ba/ac6caa255402145bc86e6d9993568a/1136_8DR-T1-TDNA-1_A23NMKMLT4_1_vs_1136_8DR-N1-BDNA-1_A23NLN3LT4_1.snv_indel.merged.vcf.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/df/4e363182542d7c944334c4534cac01/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ba/ac6caa255402145bc86e6d9993568a/1136_8DR-T1-TDNA-1_A23NMKMLT4_1_vs_1136_8DR-N1-BDNA-1_A23NLN3LT4_1.snv_indel.merged.vcf.gz.tbi Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/df/4e363182542d7c944334c4534cac01/.command.run Downloading: s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/daq/20260330_LH00527_0071_A23NMKMLT4__0ce77ad5--20260401-094121/alignments/recalibrated/1136_8DR-T1-TDNA-1_A23NMKMLT4_1/1136_8DR-T1-TDNA-1_A23NMKMLT4_1.recalibrated.bam Downloading: s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/daq/20260330_LH00527_0071_A23NMKMLT4__0ce77ad5--20260401-094121/alignments/recalibrated/1136_8DR-T1-TDNA-1_A23NMKMLT4_1/1136_8DR-T1-TDNA-1_A23NMKMLT4_1.recalibrated.bam.bai ==> STAGING COMPLETE (8 inputs) This is WhatsHap 2.8 running under Python 3.9.23 [W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_A23NMKMLT4_1.recalibrated.bam.bai [W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_A23NMKMLT4_1.recalibrated.bam.bai [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' Working on 2 samples from 2 families WARNING: Skipping duplicated position 31684999 on chromosome 'chr21' Hiding further warnings of this type, use --debug to show # Working on contig chr21 in individual 1136_8DR-N1-BDNA-1_A23NLN3LT4_1 Found 21 usable heterozygous variants (0 skipped due to missing genotypes) WARNING: Sample '1136_8DR-N1-BDNA-1_A23NLN3LT4_1' not found in any BAM/CRAM file. Found 0 reads covering 0 variants Kept 0 reads that cover at least two variants each Selected 0 most phase-informative reads covering 0 variants Best-case phasing would result in 0 non-singleton phased blocks (0 singletons). Phasing 1 sample by solving the MEC problem ... No. of phased blocks: 0 # Working on contig chr21 in individual 1136_8DR-T1-TDNA-1_A23NMKMLT4_1 Found 228 usable heterozygous variants (0 skipped due to missing genotypes) [W::hts_idx_load3] The index file is older than the data file: 1136_8DR-T1-TDNA-1_A23NMKMLT4_1.recalibrated.bam.bai Number of supplementary alignments: 0 Number of non-singleton groups: 27178 Skipped 0 groups Found 94877 reads covering 228 variants Kept 22864 reads that cover at least two variants each Selected 504 most phase-informative reads covering 110 variants Best-case phasing would result in 33 non-singleton phased blocks (0 singletons). Phasing 1 sample by solving the MEC problem ... Largest block contains 10 variants (9.1% of accessible variants) between position 44573789 and 44592359 WARNING: Ignoring existing phasing information found in input VCF (PS tag exists). # Resource usage Maximum memory usage: 0.198 GB Time spent reading BAM/CRAM: 61.6 s Time spent parsing VCF: 0.0 s Time spent selecting reads: 0.8 s Time spent phasing: 0.3 s Time spent writing VCF: 0.0 s Time spent finding components: 0.0 s Time spent on rest: 0.2 s Total elapsed time: 62.9 s