Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 100000 total bases: 15000000 Q20 bases: 14861226(99.0748%) Q30 bases: 14655147(97.701%) Read2 before filtering: total reads: 100000 total bases: 15000000 Q20 bases: 14824366(98.8291%) Q30 bases: 14547373(96.9825%) Read1 after filtering: total reads: 99324 total bases: 13751962 Q20 bases: 13696478(99.5965%) Q30 bases: 13561788(98.6171%) Read2 after filtering: total reads: 99324 total bases: 13753321 Q20 bases: 13679148(99.4607%) Q30 bases: 13512414(98.2484%) Filtering result: reads passed filter: 198648 reads failed due to low quality: 1260 reads failed due to too many N: 92 reads failed due to too short: 0 reads with adapter trimmed: 73510 bases trimmed due to adapters: 2300619 Duplication rate: 0.296% Insert size peak (evaluated by paired-end reads): 122 JSON report: rna_s1221_S42.fastp.json HTML report: rna_s1221_S42.fastp.html fastp --in1 rna_s1221_S42_1.fastq.gz --in2 rna_s1221_S42_2.fastq.gz --out1 rna_s1221_S42_1.fastp.fastq.gz --out2 rna_s1221_S42_2.fastp.fastq.gz --json rna_s1221_S42.fastp.json --html rna_s1221_S42.fastp.html --thread 12 --detect_adapter_for_pe fastp v0.23.4, time used: 3 seconds